1

BSc. Perfusion Technology

CURRICULUM FOR BSc. Perfusion Technology

1. Eligibility for admission:

A candidate seeking admission to the Bachelor of Science Degree Course in Perfusion Technology
should have passed:
.
1. Two year Pre-University examination or equivalent as recognized by
University of Health Sciences with, Physics, Chemistry and Biology as principle subjects
of study.
OR
2. Pre-Degree course from a recognized University considered as equivalent by KUHS,
(Two years after ten years of schooling) with Physics, Chemistry and Biology as principal
subjects of study.
OR

3. Any equivalent examination recognized by the KUHS,Kerala for the above purpose with Physics,
Chemistry and Biology as principal subjects of study.
OR
4. The vocational higher secondary education course conducted by Vocational Higher
Secondary Education, Government of Kerala with five subjects including Physics, Chemistry,
Biology and English in addition to vocational subjects conducted is considered equivalent to
plus TWO examinations of Government of KeralaPre University Course.

Age limit

3. Duration of the course:

Duration shall be for a period of four years including one year clinicalP training..
4. Medium of instruction:
The medium of instruction and examination shall be in English.
5. Scheme of examination:
There shall be three examinations one each at the end of 1 st , 2nd and 3rd year.
6. Attendance
Every candidate should have attended at least 80% of the total number of classes conducted in an academic
year from the date of commencement of the term to the last working day as notified by university in each of
the subjects prescribed for that year separately in theory and practical. Only such candidates are eligible to
appear for the university examinations in their first attempt. Special classes conducted for any purpose
shall not be considered for the calculation of percentage of attendance for eligibility. A candidate lacking in
prescribed percentage of attendance in any subjects either in theory or practical in the first appearance will
not be eligible to appear for the University Examination in that subject .
7. Internal Assessment (IA) :
Theory - 20 marks.
Practical - 20 marks. [Lab work- 12 marks and Record-8 marks ]
There shall be a minimum of two periodical tests preferably one in each term in theory and practical of
each subject in an academic year. The average marks of the two tests will be calculated and reduced to 20.
The marks of IA shall be communicated to the University at least 15 days before the commencement of the
University examination. The University shall have access to the records of such periodical tests. The marks
of the internal assessment must be displayed on the notice board of the respective colleges with in a
fortnight from the date test is held. If a candidate is absent for any one of the tests due to genuine and
satisfactory reasons, such a candidate may be given a re-test. within a fortnight.
* There shall be no University Practical Examination in First year.
8. Subject and hours of teaching for Theory and Practical
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The number of hours of teaching theory and practical subject wise in first year, second year and third year
are shown in Table-I, II and III
Table - I Distribution of Teaching Hours in First Year Subjects
Main Subjects
Theory Practical No
Total No. of
SL NO Subject No Of Of Hrs
Hours
Hrs
Human Anatomy 70
1 20 90

90
2 Physiology 70 20

Biochemistry 90
3 70 20

Pathology-[Clinical
pathology,Haemotology
90
4 & 70 20
Blood –Banking

Microbiology 90
5 70 20

100 450
Total 350

The classes in main subjects are to be held from Monday to Thursday. On Fridays and Saturdays students
shall work in hospitals
Hospital posting –470Hours- Fri day 9am – 1pm and 2pm - 4-30 pm Saturday 9am - 1pm
Table - II Distribution of Teaching Hours in Second Year Subjects
Main Subjects
Theory Practical No Of
SL Clinical Total No. of
Subject No Of Hrs
NO Posting Hours
Hrs
Medicine relevant to perfusion
1 technology 50 -- -- 50

Section A Applied Pathology 60

2 30 30 --

Section B Applied Microbiology 60

3 30 30

50
4 Applied Pharmacology 50 --

Introduction to Perfusio
ntechnology 830
5 80 100 650

240 160 1050

Total 650
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Table - III Distribution of Teaching Hours in Third Year Subjects

Main Subjects
Theory
SL Practical Clinical Total
Subject No. of
No No. of posting No. of
Hours
Hours Hours
Perfusion 350
1 50 50 250
Technology – Clinical
Perfusion
350
2 Technology – Applied 50 50 250

Perfusion 350
3 50 50 250
Technology – Advanced
150 150 750 1050

9. Schedule of Examination:
The university shall conduct two examinations annually at an interval of not less than 4 to 6 months as
notified by the university from time to time. A candidate who satisfies the requirement of attendance,
progress and conduct as stipulated by the university shall be eligible to appear for the university
examination. Certificate to that effect shall be produced from the Head of the institution along with the
application for examination and the prescribed fee.
10. Scheme of Examination
There shall be three examinations, one each at the end of I, II and III year. Distribution of Subjects and
marks for First Year, Second year & Third year University theory and practical Examinations are shown in
the Table – IV,V & VI.
First year examination:
The University examination for 1st year shall consist of only theory examination and there shall be no
University Practical Examination.
Second & Third year examination:
The University examination for 2nd and 3rd year shall consist of Written Examination & Practical.
Written Examinations consists of 02 papers in the 2nd Year 02 papers in the 3rd Year.
Practical examination:
Two practical examinations, at the end 2nd Year and one practical examination at the
end of the 3rd year.

TABLE-IV
Distribution of Subjects and marks for First Year University theory Examination
I .A
Main Subjects* Written Paper Total
Sl No Theory

Duration Marks Marks Marks

Basic Anatomy
1 3 hours 80 20 100
[Including Histology]

2 P hy s i ol ogy 3 hours 80 20 100

3 Biochemistry 3 hours 80 20 100

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Pathology 20
4 3 hours 80 100

5 Microbiology 3 hours 80 20 100

100

Note * I A = Internal Assessment

Main Subjects shall have University Examination.
There Shall be no University Practical Examination
.
TABLE – V
Distribution of Subjects and marks for Second Year Examination.

Practicals
Theory

Sub Sub Grand

Paper Subjects Theory Vivavoca
IA Total Practicals IA Total Total

Section A – Applied
Pathology 50
Section B - Applied 50 30 20 150 40 10 50 200
I Microbiology

Introduction to
Perfusion
100 30 20 150 40 10 50 200
Technology
II

III Pharmacology 80 -- 20 100 No Practicals 100

Medicine relevant to
80 -- 20 100 No Practicals 100
IV Perfusion Technology

TABLE – VI
Distribution of Subjects and marks for Third Year Examination.

Grand
Theory Practicals Total

Sub
Paper subjects Theory Vivavoca IA Practicals IA
Sub Total
Total
Perfusion
I Technology – 100 30 20 150 120 30 150 600
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Clinical (40=40=40) (10=10=10)

Perfusion
II Technology – 100 30 20 150
Applied
Perfusion
Technology –
III Advanced 100 30 20 150

11. Pass criteria

11.1. First year examination.
a. Main Subjects: A candidate is declared to have passed in a subject, if he/she secures, 50% of marks in
University Theory exam and internal assessment added together.

11.2. Second and Third year Examination a. Main Subjects: A candidate is declared to have passed
the Examination in a subject if he/she secures 50% of the marks in theory and 50% in practical separately.
For a pass in theory, a candidate has to secure a minimum of 40% marks in the University conducted
written examination, and 50% in aggregate in the University conducted written examination, internal
assessment and Viva-Voce added together and for pass in Practical, a candidate has to secure a minimum of
40% marks in the university conducted Practical/Clinical examination and 50% in aggregate i.e. University
conducted Practical/Clinical and Internal Assessment.
In the third year a candidate is declared to have passed only if he/she passes all the three theory papers and
one practical examination in a single attempt failing which were in the candidate fails in one or more theory
papers and or practical examination he/she will have to re appear for all the 3 theory papers and the
practical examination in the subsequent attempt. .
12. Carry over benefit
12.1 First year examination:
A candidate who fails in any two of the five main subjects of first year shall be permitted to carry over
those subjects to second year. However, he/se must pass the carry over subjects before appearing for
second year examination; otherwise he/she shall not permitted to proceed to third year.
12.2. Second year examination.
A candidate is permitted to carry over any one main subject to the third year but shall pass this subject
before appearing for the third year examination
13. Declaration Of Class
a. A candidate having appeared in all the subjects in the same examination and passed that examination in
the first attempt and secures 75% of marks or more of grand total marks prescribed will be declared to have
passed the examination with Distinction.
b. A candidate having appeared in all subjects in the same examination and passed that examination in the
first attempt and secures 60% of marks or more but less than 75% of grand total marks prescribed will be
declared to have passed the examination in First Class.
c. A candidate having appeared in all the subjects in the same examination and passed that examination in
the first attempt and secures 50% of marks or more but less than 60% of grand total marks prescribed will
be declared to have passed the examination in Second Class.
d. A candidate passing the university examination in more than one attempt shall be placed in Pass class
irrespective of the percentage of marks secured by him/her in the examination.
e. The marks obtained by a candidate in the subsidiary subjects shall not be considered for award of Class
or Rank. [Please note fraction of marks should not be rounded off clauses (a), (b) and (c)]
14. Eligibility for the award of Degree:
A candidate shall have passed in all the subjects of first, second and third year to be eligible for award of
degree.
15. Distribution of Type of Questions and Marks for Various Subjects
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THEORY

SUBJECTS HAVING MAXIMUM MARKS = 100

TYPE OF QUESTION MARKS FOR EACH QUESTION

NUMBER OF QUESTIONS
3 (2x10) 10
ESSAY TYPE
SHORT ESSAY TYPE 12 (10 × 5 ) 5

SHORT ANSWER TYPE 12 (10 × 3) 3

SUBJECTS HAVING MAXIMUM MARKS = 80

TYPE OF QUESTION MARKS FOR EACH QUESTION

NUMBER OF QUESTIONS
3 (2x10) 10
ESSAY TYPE
SHORT ESSAY TYPE 8(6X5) 5
SHORT ANSWER TYPE 12 (10 × 3) 3

SUBJECTS HAVING MAXIMUM MARKS = 60

TYPE OF QUESTION MARKS FOR EACH QUESTION

NUMBER OF QUESTIONS
3 (2x10) 10
ESSAY TYPE
SHORT ESSAY TYPE 7(5X5) 5
SHORT ANSWER TYPE 7(5X3) 3

SUBJECTS HAVING MAXIMUM MARKS = 50

TYPE OF QUESTION MARKS FOR EACH QUESTION

NUMBER OF QUESTIONS
3 (2x10) 10
ESSAY TYPE
SHORT ESSAY TYPE 5(3X5) 5
SHORT ANSWER TYPE 7(5X3) 3
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Syllabus for First year BSc. Perfusion Technology

A ANATOMY
No. of theory classes: 70 hours
No. of practical classes : 20 hours
Introduction: human body as a whole
Theory:
Definition of anatomy and its [Link] of location, positions and planes
Cell and its organelles
Epithelium-definition, classification, describe with examples, function
Glands- classification, describe serous & mucous glands with examples
Basic tissues – classification with examples
Practical: Histology of types of epithelium
Histology of serous, mucous & mixed salivary gland
Locomotion and support
Theory:
Cartilage – types with example & histology
Bone – Classification, names of bone cells, parts of long bone, microscopy of compact bone, names of all
bones, vertebral column, intervertebral disc, fontanelles of fetal skull
Joints – Classification of joints with examples, synovial joint (in detail for radiology)
Muscular system: Classification of muscular tissue & histology
Names of muscles of the body
Practical: Histology of the 3 types of cartilage
Demo of all bones showing parts, radiographs of normal bones & joints
Histology of compact bone (TS & LS)
Demonstration of all muscles of the body
Histology of skeletal (TS & LS), smooth & cardiac muscle
3. Cardiovascular system
Theory:
Heart-size, location, chambers, exterior & interior
Blood supply of heart
Systemic & pulmonary circulation
Branches of aorta, common carotid artery, subclavian artery, axillary artery, brachial artery, superficial
palmar arch, femoral artery, internal iliac artery
Peripheral pulse
Inferior venacava, portal vein, portosystemic anastomosis Great saphenous vein ,Dural venous sinuses
Lymphatic system- cister na chyli & thoracic duct
Histology of lymphatic tissues
Names of regional lymphatics, axillary and inguinal lymph nodes in brief
Practical:
Demonstration of heart and vessels in the body
Histology of large artery, medium sized artery & vein, large vein
Microscopic appearance of large artery, medium sized artery & vein, large vein pericardium
Histology of lymph node, spleen, tonsil & thymus
Normal chest radiograph showing heart shadows
Normal angiograms
4. Gastro-intestinal system
Theory:
Parts of GIT, Oral cavity (lip, tongue (with histology), tonsil, dentition, pharynx, salivary glands,
Waldeyer’s ring)
Oesophagus, stomach, small and large intestine, liver, gall bladder, pancreas
Radiographs of abdomen
5. Respiratory system
Parts of RS, nose, nasal cavity, larynx, trachea, lungs, bronchopulmonary segments
Histology of trachea, lung and pleura,Names of paranasal air sinuses
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Practical: Demonstration of parts of respiratory system.

Normal radiographs of chest
Histology of lung and trachea
6. Peritoneum
Theory: Description in brief
Practical: Demonstration of reflections
7. Urinary system
Kidney, ureter, urinary bladder, male and female urethra
Histology of kidney, ureter and urinary bladder
Practical: Demonstration of parts of urinary system
Histology of kidney, ureter, urinary bladder
Radiographs of abdomen-IVP, retrograde cystogram
8. Reproductive system
Theory:
Parts of male reproductive system, testis, vas deferens, epididymis, prostate (gross & histology)
Parts of female reproductive system, uterus, fallopian tubes, ovary (gross & histology)
Mammary gland – gross
Practical: demonstration of section of male and female pelves with organs in situ
Histology of testis, vas deferens, epididymis, prostate, uterus, fallopian tubes, ovary
Radiographs of pelvis – hysterosalpingogram
9. Endocrine glands
Theory:
Names of all endocrine glands in detail on pituitary gland, thyroid gland, parathyroid gland,
suprarenal glad – (gross & histology)
Practical: Demonstration of the glands
Histology of pituitary, thyroid, parathyroid, suprarenal glands
10. Nervous system
Theory:
[Link] of [Link], cerebellum, midbrain, pons, medulla oblongata, spinal cord with
spinal nerve (gross & histology).Meninges, Ventricles & cerebrospinal fluid,,Names of basal nuclei ,Blood
supply of [Link] nerves,Sympathetic trunk & names of parasympathetic ganglia
Practical: Histology of peripheral nerve & optic nerve
Demonstration of all plexuses and nerves in the body
Demonstration of all part of brain
Histology of cerebrum, cerebellum, spinal cord
Sensory organs:
Theory:
Skin: Skin-histology
Appendages of skin
Eye: parts of eye & lacrimal apparatus,Extra-ocular muscles & nerve supply
Ear: parts of ear- external, middle and inner ear and contents
Practical: Histology of thin and thick skin
Demonstration and histology of eyeball,Histology of cornea & retina
Embryology
Theory:
Spermatogenesis & oogenesis,Ovulation, fertilization,Fetal circulation,Placenta
Internal Assessment
Theory - Average of two exams conducted. 20
Practicals: Record & Lab work* 10
* There shall be no University Practical Examination and internal assessment marks secured in
Practicals need not be sent to the University.
Scheme of Examination Theory
There shall be one theory paper of three hours duration carrying 80 marks. Distribution of type
of questions and marks for Anatomy shall be as given under.
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Type of Questions No. of Questions Marks Sub-total

Long Essay (LE) 3(To attempt 2) 2 x 10 20

Short Essay (SE) 8 (To attempt 6) 6x5 30

Short Answer (SA) 12 (To Attempt 10) 10 x 3 30

Total Marks 80

NO PRACTICAL EXAMINATION
REFERENCE BOOKS
Anatomy
1 William Davis (P) understanding Human Anatomy and Physiology MC Graw Hill
2. Chaursia –A Text book of Anatomy T.S. Ranganathan – A text book of Human Anatomy
3. Fattana, Human anatomy (Description and applied) Saunder’s & C P Prism Publishers, Bangalore – 1991
4. ESTER . M. Grishcimer, Physiology & Anatomy with Practical Considerations, J.P. Lippin Cott.
Philadelphia
5. Essential of Human embryology, Bhatnagar revised edition, Orient Longman PVT Ltd.

Syllabus for First year BSc. Perfusion Technology

B PHYSIOLOGY

Theory 70 hours
Practical 20hours
Introduction – composition and function of [Link] blood cells – Erythropoiesis , stages of
differentiation function , count physiological [Link] –structure , functions , concentration
physiological [Link] of Estimation of [Link] blood cells – Production , function, life span,
count, differential count .Platelets – Origin, normal count, morphology functions..Plasma Proteins
Production, concentration , types, albumin, globulin, Fibrinogen, Prothrombin functions..Haemostasis &
Blood [Link] – Definition, normal haemostasis, clotting factors, mechanism of clotting,
disorders of clotting factors.
Blood Bank
Blood groups – ABO system, Rh [Link] grouping & [Link] system – Rh factor,
Rh incompatibility.
Blood transfusion – Indication, universal donor and recipient [Link] criteria of a blood donor.
transfusion reactions Anticoagulants – Classification,Examples and uses
Anaemias : Classification – morphological and etilogical. effects of anemia on body
Blood indices – Colour index , MCH, MCV, MCHC
Erythrocyte sedementation Rate (ESR) and Paced cell [Link] values, Definition . determination,
Blood Volume -Normal value ,determination of blood volume and regulation of blood volume
Body fluid – pH, normal value, regulation and variation
Lymph – lymphoid tissue formation, circulation, composition and function of lymph
Cardiovascular system
Heart – Physiological Anatomy, Nerve [Link] of Cardiac muscle,Cardiac cycle-systole,diastole.
Intraventricular pressure [Link] Output – only definition
Heart sounds Normal heart sounds Areas of [Link] Pressure – Definition, normal value,
clinical measurement of blood [Link] variations, regulation of heart rate, cardiac shock,
hypotension, [Link] – Jugular, radial pulse, Triple response Heart sounds – Normal heart
sounds, cause characteristics and signification. Heart rate
Electrocardiogram (ECG) –significance.
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Digestive System - Physiological anatomy of Gastro intestinal tract, Functions of digestive

System. Salivary glands Stucture and functions. Deglutination –stages and regulation
Stomach – structure and [Link] secretion – Composition function regulation of gastric juice
[Link] – structure, function, composition, regulation of pancreatic juice
Liver – functions of [Link] secretion, composition, function regulation of bile secretion .Bilirubin
metabolism types of bilirubin, Vandernberg reaction, Jaundice- types, [Link] bladder – functions
Intestine – small intestine and large intestine
Small intestine –Functions- Digestive, absorption ,movements.
Large intestine – Functions, Digestion and absorption of Carbohydrates,Proteins, Fats,[Link]
Respiratory system
Functions of Respiratory system, Physiological Anatomy of Respiratory system,Respiratory tract,
Respiratory Muscles, Respiratory organ-lungs, Alveoli, Respiratory membrane, stages of
[Link] of normal and rigorous respiration. Forces opposing and favoring expansion of the
lungs. Intra pulmonary pleural pressure, surface tension, recoil tendency of the wall.
Transportation of Respiratory gases :Transportation of Oxygen : Direction, pressure gradient, Forms of
transportation, Oxygenation of Hb. Quantity of Oxygen [Link] volumes and capacities
Regulation of respiration what? Why? How? Mechanisms of Regulation, nervous and chemical regulation.
Respiratory centre. Hearing Brier, Reflexes.
Applied Physiology and Respiration : Hypoxia, Cyanosis, Asphyxia, Dyspnea, Dysbarism, Artificial
Respiration, Apnoea.
Endocrine System - Definition Classification of Endocrine glands & their Hormones .Properties of
Hormones .
Thyroid gland hormone – Physiological, Anatomy, Hormone scerated, Physiological function,
regulation of secretion. Disorders – hypo and hyper secretion of hormone
Adrenal gland, Adrenal cortex physiologic anatomy of adrenal gland, Adrenal cortex, cortical hormones –
functions and [Link] medulla – Hormones , regulation and secretion. Functions of Adrenaline
and nor [Link] hormones – Anterior and posterior pituitary hormones, secretion , function
Pancreas – Hormones of [Link] – secretion, regulation, function and [Link] mellitus –
Regulation of blood glucose level
Parathyroid gland – function, action, regulation of secretion of parathyroid [Link] – function
and action
Special senses
Vision – structure of eye. Function of different [Link] of retina
Hearing structure and function of can mechanism of hearing
Taste – Taste buds functions . Smell physiology, Receptors.
Nervous system
Functions of Nervous system, Neurone structure, classification and properties. Neuroglia,
nerve fiber, classification ,conduction of impulses continuous and saltatory. Velocity of impulse
transmission and factors affecting. Synapse – structure, types, properties.
Receptors – Definition, classification ,properties. Reflex action – unconditioned properties of reflex action.
Babinski’s sign. Spinal cord nerve tracts. Ascending tracts, Descending tracts – pyramidal tracts –
Extrapyramidal tracts. Functions of Medulla, pons, Hypothalamic disorders. Cerebral cortex lobes and
functions, Sensory cortex, Motor cortex,Cerebellum functions of [Link] ganglion-functions.
[Link] Spinal Fluid(CSF) : formation, circulation, properties, composition and functions lumbar
[Link] Nervous System : Sympathetic and parasympathetic distribution and functions and
comparison of functions.
Excretory System
Excretory organs
Kidneys: Functions of kidneys structural and functional unit nepron, vasarecta, cortical and
juxtamedullary nephrons – Comparision, Juxta Glomerular Apparatus –Structure and function.
Renal circulation [Link] of Urine formation : Ultrafiltration criteria for filtration GFR,
Plasma fraction, EFP, factors effecting EFR. Determination of GFR selective reabsorption – sites of
reabsorption ,substance reabsorbed, mechanisms of reabsorption Glucose, urea. H + Cl aminoacids etc.
TMG, Tubular lead, Renal threshold % of reabsorption of different substances, selective e secretion.
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Properties and composition of normal urine, urine output. Abnormal constituents in urine , Mechanism of
urine concentration..Counter – Current Mechanisms : Micturition, Innervation of Bladder,
Cysteurethrogram.
Diuretics : Water, Diuretics, osmotic diuretics, Artificial kidney Renal function tests – plasma
clearance Actions of ADH, Aldosterone and PTH on kidneys. Renal function tests
Reproductive system
Function of Reproductive system, Puberty, male reproductive system. Functions of testes,
spermatogenesis site, stages, factors influencing semen. Endocrine functions of testes
Androgens – Testosterone structure and functions. Female reproducive syustem. Ovulation,
menstrual cycle. Physiological changes during pregnancy, pregnancy test. Lactation :
Composition of milk factors controlling lactation.
Muscle nerve physiology
Classification of muscle, structure of skeletal muscle, Sarcomere contractile proteins, Neuromuscular
junction. Transmission across, Neuromuscular junction. Excitation contraction coupling. Mechanism of
muscle contraction muscle tone, fatigue Rigour mortis
Skin -structure and function
Body temperature measurement, Physiological variation, Regulation of body Temperature by physical
chemical and nervous mechanisms .Role of Hypothalamus, Hypothermia and fever.
Practicals
Haemoglobinometry,White Blood Cell count,Red Blood Cell count,Determination of Blood Groups
Leishman’s staining and Differential WBC count,Determination of packed cell Volume,Erythrocyte
sedimentation rate [ESR],Calculation of Blood indices,Determination of Clotting Time, Bleeding Time
Blood pressure Recording,Auscultation for Heart Sounds
Artificial Respiration,Determination of vital capacity
Internal Assessment
Theory - Average of two exams conducted. 20
Practicals: Record & Lab work* 10
* There shall be no University Practical Examination and internal assessment marks secured in
Practicals need not be sent to the University.
Scheme of Examination Theory

Type of Questions No. of Questions Marks Sub-total

Long Essay (LE) 3(To attempt 2) 2 x 10 20

8 (To attempt 6)
Short Essay (SE) 6x5 30

Short Answer (SA) 12 (To Attempt 10) 10 x 3 30

Total Marks 80

NO PRACTICAL EXAMINATION
REFERENCE BOOKS
Physiology
1. Guyton (Arthur) Text Book of Physiology. Latest Ed. Prism publishers
2. Chatterjee(CC) Human Physiology Latest Ed. Vol-1, Medical Allied Agency
3. Choudhari (Sujith K) Concise Medical Physiology Latest Ed. New Central Book,
4. Ganong (William F) Review of Medical Physiology. Latest Ed . Appleton
Syllabus for First year BSc. Perfusion Technology
C BIOCHEMISTRY

No. Theory classes : 70hours

No. of practical classes : 20 hours
Theory:
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Specimen collection : Pre-analytical variables

Collection of blood,Collection of CSF & other fluids,Urine collection,Use of preservatives,Anticoagulants
Introduction to Laboratory apparatus
Pipettes- different types (Graduated, volumetric, Pasteur, Automatic etc.,),Calibration of glass pipettes
Burettes, Beakers, Petri dishes, depression plates.,Flasks - different types )Volumetric, round bottomed,
Erlemeyer conical etc.,),Funnels – different types (Conical, Buchner etx.,),Bottles – Reagent bottles –
graduated and common, Wash bottles – different type,Specimen bottles etc.,Measuring cylinders, Porcelain
dish,Tubes – Test tubes, centrifuge tubes, test tube draining rack,Tripod stand, Wire gauze, Bunsen burner.
Cuvettes, significance of cuvettes in colorimeter, cuvettes for visible and UV range, cuvetteholders Racks –
Bottle, Test tube, Pipette,Dessicator, Stop watch, rimers, scissors,Dispensers – reagent and sample
Any other apparatus which is important and may have been missed should also be covered
Maintenance of lab glass ware and apparatus:,Glass and plastic ware in Laboratory
*use of glass: significance of boro silicate glass ; care and cleaning of glass ware, different cleaning
solutions of glass,care and cleaning of plastic ware, different cleaning solutions .
3. Instruments (Theory and demonstration) Diagrams to be drawn,Water bath: Use, care and maintenance
Oven & Incubators : Use, care and maintenance.,Water Distilation plant and water deionisers. Use, care
and maintenance,Refrigerators, cold box, deep freezers – Use, care and maintenance,Reflux condenser :
Use, care and maintenance,Centrifuges (Theory and demonstration) Diagrams to be drawn
Definition, Principle, svedberg unit, centrifugal force, centrifugal field rpm,[Link] of G to rpm and
vice versa.,Different types of centrifuges,Use care and maintenance of a centrifuge
Laboratory balances [Theory & Practicals) Diagrams to be drawn
Manual balances: Single pan, double pan, trip balance,Direct read out electrical balances.
Use care and maintenance. Guideline to be followed and precautions to be taken while
Weighing,Weighing different types of chemicals, liquids. Hygroscopic compounds etc.
Colorimeter and spectrophotometer (Theory and Practicals) Diagrams to be drawn,Principle, Parts
Diagram.,Use, care and maintenance.
pH meter (Theory & practicals) Diagrams to be drawn,principle, parts, Types of electrods, salt bridge
solution.,Use, care and maintenance of Ph meter and electrodes,Guidelines to be followed and precautions
to be taken while using pH meter
4. Safety of measurements
5. Conventional and SI units
6. Atomic structure,Dalton’s theory, Properties f electrons, protons, neutrons, and nucleus, Rutherford’s
model of,atomic structure, Bohr’s model of atomic structure, orbit and orbital, Quantum numbers,
Heisenberg’s uncertainly principle,Electronic configuration – Aufbau principle, Pauli’s exclusion principle,
etc.,m,Valency and bonds – different types of strong and weak bonds in detail with examples
Theory & Practicals for all the following under this section
Molecular weight, equivalent weight of elements and compounds, normality molarity
Preparation of molar solutions (mole/litre solution) eg: 1 M Nacl, 0.15 M NaCL,1 M NaOH, 0.1 M HCl,
0.1 M H 2S04 etc.,
preparation of normal solutions. eg., IN Na2CO3, O IN Oxalic acid, 0.1 N HCl, 0.1N H2504,,0.66 N
H2S04 etc.,
Percent solutions. Preparation of different solutions – v/v w/v (solids, liquids and acids),Conversion of a
percent solution into a molar solution
Dilutions
Diluting solutions: eg. Preparation of 0.1 N NaCl from 1 N NaCl from 2 NHCl etc., Preparing working
standard from stock standard, Body fluid dilutions, Reagent dilution techniques,calculating the dilution of a
solution, body fluid reagent etc., Saturated and supersaturated solutions.,Standard solutions. Technique for
preparation of standard solutions eg: Glucose, urea, etc.,Significance of volumetric flask in preparaing
standard solutions. Volumetric flasks of different sizes, Preparation of standard solutions of deliquesent
compounds (CaCl2, potassium carbonate, sodium hydroxide etc.,) Preparation of standards using
conventional and Sl units Acids, bases, salts and indicators.
Acids and Bases: Definition, physical and chemical properties with [Link] concept of acids
and bases, Lowery – Bronsted theory of acids and bases,classification of acids and bases. Different between
bases and alkali, acidity and basicity, monoprotonic and polyprotonic acids and bases
Concepts of acid base reaction, hydrogen ion concentration, Ionisation of water, buffer, Ph value of a
solution, preparation of buffer solutions using Ph meter.
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Salts: Definition, classification, water of crystallization – definition and different types, deliquescent and
hygroscopic salts
Acid- base indicators: (Theory and Practicals)
Theory – Definition, concept, mechanism of dissociation of an idicator, colour change of an indicator in
acidic and basic conditions, use if standard buffer solution and indicators for Ph determinations, preparatin
and its application, list of commonly used indicators and their Ph range, suitable pH indicators used in
different titrations, universal indicators
Practicals – Titration of a simple acid and a base (Preparation of standard solution of oxalic acid and using
this solution finding out the normality of a sodium hydroxide soslution . Acid to be titrated using this base)
Calculation of normality of an acid or a base after titration, measurement of hydrogen ion concentration
Quality control : Accuracy,Precision,Specificity,Sensitivity,Limits of error allowable in laboratory,
Percentage error,Normal values and Interpretations
Special Investigations : Serum Electrophoresis
Immunoglobulins
Drugs : Digitoxin, Theophyllines
Regulation of Acid Base status:Henderson Hasselback Equations,Buffers of the fluid,pH
Regulation,Disturbance in acid Base Balance
Anion Gap,Metabolic acidosis,Metabolic acidosis,Metabolic alkalosis,Respiratory acidosis,Respiratory
alkalosis,Basic Principles and estimation of Blood Gases and pH,Basic principles and estimation of
Electrolytes,Water Balance,Sodium regulation,Bicarbonate buffers,Nutrition, Nutritional support with
special emphasis on parental nutrition.,Calorific Value,Nitrogen Balance
Respiratory Quotient,Basal metabolic rate,Dietary Fibers,Nutritional importance of lipids, carbohydrates
and proteins,Vitamins
PRACTICALS
Analysis of Normal Urine,Composition of urine,Procedure for routine screening,Urinary screening for
inborn errors of metabolism,Common renal disease,Urinary calculus,Urine examination for detection of
abnormal constituents,Interpretation and Diagnosis through charts,Liver Function tests,Lipid Profile
Renal Function test,Cardiac markers,Blood gas and Electrolytes
4. Estimation of Blood sugar, Blood Urea and electrolytes
5. Demonstration of Strips,Demonstration of Glucometer
Internal Assessment
Theory - Average of two exams conducted. 20
Practicals: Record & Lab work* 10
* There shall be no University Practical Examination and internal assessment marks secured in
Practicals need not be sent to the University.
Scheme of Examination Theory

Type of Questions No. of Questions Marks Sub-total

Long Essay (LE) 3(To attempt 2) 2 x 10 20

30
8 (To attempt 6)
Short Essay (SE) 6x5
Short Answer (SA) 12 (To Attempt 10) 10 x 3 30
Total Marks 80

NO PRACTICAL EXAMINATION
REFERENCE BOOKS
Biochemistry
1. Varley – Clinical chemistry
2. TEITZ – Clinical chemistry
 14

3. Kaplan – Clinical chemistry

4. Ramakrishna(S) Prasanna(KG), Rajna ® Text book of Medical Biochemistry Latest Ed Orient longman
Bombay –1980
5. Vasudevan (DM) Sreekumari(S) Text book of Biochemistry for Medical students ,Latest Ed
6. DAS(Debajyothi) Biochemistry Latest ED Academic, Publishers, Culcutta – 1992
7. Text Book of Medical Biochemistry – 3rd Edition, Orient Longman PVT Ltd
8. Practical Biochemistry for Medical Students – Rajagopal, Orient Longman PVT Ltd
Syllabus for First year [Link]. Perfusion Technology
D PATHOLOGY

Histo Pathology ,Clinical Pathology, Haematology and Blood Banking

Theory – 70 hours
Practical – 20 hours
HistoPathology - Theory
- Introduction to Histo Pathology, Receiving of Specimen in the [Link] Techniques
- Mounting Techniques – various Mountants, Maintenance of records and filing of the slides.
- Use & care of Microscope, Various Fixatives, Mode of action, Preparation and Indication.
- Bio-Medical waste management, Section Cutting, Tissue processing for routine paraffin sections
- Decalcification of Tissues. Staining of tissues - H& E Staining,Bio-Medical waste management
Clinical Pathology – Theory
- Introduction to Clinical Pathology, Collection, Transport, Preservation, and Processing of various clinical
Specimens. Urine Examination – Collection and Preservation of [Link], chemical, Microscopic
[Link] of body [Link] of cerebro spinal fluid (CSF).Sputum Examination.
- Examination of feces
Haematology – Theory
- Introduction to Haematology,Normal constituents of Blood, their structure and [Link] of
Blood sample. Various Anticoagulants used in [Link] instruments and glassware used in
Haematology, Preparation and use of glassware
- Laboratory safety [Link] units and conventional units in Hospital Laboratory
- Hb,[Link]. Normal [Link] Time, Clotting Time, Prothrombin Time, Activated Partial
Thromboplastin Time.

Blood Bank
Introduction
Blood grouping and Rh [Link] matching
PRACTICALS
- Urine Examination. [Link]. Microscopic
- Blood Grouping Rh [Link] Estimation,Packed Cell Volume[PCV], Erythrocyte Sedimentation
rate{ESR]. Bleeding Time, Clotting Time.
Internal Assessment
Theory - Average of two exams conducted. 20
Practicals: Record & Lab work* 10
* There shall be no University Practical Examination and internal assessment marks secured in
Practicals need not be sent to the University.
Scheme of Examination Theory
There shall be one theory paper of three hours duration carrying 80 marks. Distribution
of type of questions and marks for Pathology shall be as given under.

Type of Questions No. of Questions Marks Sub-total

Long Essay (LE) 3(To attempt 2) 2 x 10 20

30
 15

8 (To attempt 6)
Short Essay (SE) 6x5
Short Answer (SA) 12 (To Attempt 10) 10 x 3 30
Total Marks 80

NO PRACTICAL EXAMINATION

REFERENCE BOOKS
Pathology –
1. Culling Histopathology techniques
2. Bancroft Histopathology techniques
3. Koss – cytology
4. Winifred greg – Diagnostic cytopathology
5. Orell – Cyto Pathology
6. Todd & Sanford Clinical Diagnosis by laboratory method
7. Dacie & Lewis – Practical Haematology
8. Ramanic Sood, Laboratory Technology (Methods and interpretation) 4th Ed.
J.P. Bros, New Delhi –1996)
9. Satish Gupta Short text book of Medical Laboratory for technician J.P. Bros, New Delhi – 1998
[Link] K.N. Clinical Pathology and Bacteriology 8th Ed, J.P. Bros, New Delhi-1991.
11. Krishna - Text book of Pathology, Orient Longman PVT Ltd. Bacteriology 8th Ed, J.P.
Syllabus for First year BSc. Perfusion Technology
E Microbiology

Objective : - This course introduces the principles of Microbiology with emphasis on applied
aspects of Microbiology of infectious diseases particularly in the following areas Principles &
practice of sterilization [Link] and despatch of specimens for routine microbiological
[Link] of commonly done bacteriological and serological investigations.
Control of Hospital [Link] waste [Link] schedule
Theory - 70 hours
1. Morphology 4 hours
Classification of microorgaisms, size, shape and structure of bacteria. Use of microscope in the study of
bacteria.
2. Growth and nutrition 4 hours
Nutrition, growth and multiplications of bacteria, use of culture media in diagnostic bacteriology.
3. Sterilisation and Disinfection 4 hours
Principles and use of equipments of sterlization namely Hot Air oven, Autoclave and serum inspissrator.
Pasteurization, Anti septic and [Link] sensitivity test
4. Immunology 6 hours
Immunity Vaccines, Types of Vaccine and immunization [Link] and interpretation of
commonly done serological tests namely Widal,VDRL, ASLO, CRP, RF & ELISA. Rapid tests for HIV
and HbsAg(Technical details to be avoided)
5. Systematic Bacteriology 20 hours
Morphology, cultivation, diseases caused ,laboratory diagnosis including specimen collection of the
following bacteria( the classification, antigenic structure and pathogenicity are not to be taught)
Staphyloccci, Streptococci, Pneumococci, Gonococci, Menigococci, Cdiphtheriae, Mycobacteria,
Clostridia, Bacillus, Shigella, Salmonella, Esch coli,Klebsiella, Proteus,vibrio cholerae, Pseudomonas &
Spirochetes
6. Parasitology 10 hours
Morphology, life cycle, laboratory diagnosis of following parasites
 16

E. histolytica, Plasmodium, Tape worms, Intestinal nematodes

7. Mycology 4 hours
Morphology, diseases caused and lab diagnosis of following [Link], Cryptococcus, Dermatophytes
,opportunistic fungi.
8. Virology 10 hours
General properties of viruses, diseases caused, lab diagnosis and prevention of following viruses, Herpes,
Hepatitis, HIV, Rabies and Poliomyelitis.
9. Hospital infection Causative agents, transmission methods, investigation,prevention and control Hospital
infection. 4 hours
10. Principles and practice Biomedical waste management 4 hours
Practical 20 hours
Compound Microscope.
Demonstration and sterlization of equipments – Hot Air oven, Autoclave, Bacterial [Link] of
commonly used culture media, Nutrient broth, Nutrient agar, Blood agar,Chacolate agar, Mac conkey
medium, LJ media, Robertson Cooked meat media, Potassium tellurite media with growth, Mac with LF &
NLF, NA with staph Antibiotic susceptibility test
Demonstration of common serological tests – Widal, VRDL, ELISA. Grams stain .Acid Fast staining
Stool exam for Helminthic ova. Anaerobic culture methods
Visit to hospital for demonstration of Biomedical waste mangement.
Internal Assessment
Theory - Average of two exams conducted. 20
Practicals: Record & Lab work* 10
* There shall be no University Practical Examination and internal assessment marks secured in
Practicals need not be sent to the University.
Scheme of Examination Theory
There shall be one theory paper of three hours duration carrying 80 marks. Distribution of type
of questions and marks for Microbiology shall be as given under.

Type of Questions No. of Questions Marks Sub-total

Long Essay (LE) 3(To attempt 2) 2 x 10 20

30
8 (To attempt 6)
Short Essay (SE) 6x5
Short Answer (SA) 12 (To Attempt 10) 10 x 3 30
Total Marks 80

NO PRACTICAL EXAMINATION
REFERENCE BOOKS
Microbiology
1. Anathanarayana & Panikar Medical Microbioloty
2. Roberty Cruckshank – Medical Microbiology – The Practice of Medical Mircrobiology
3. Chatterjee – Parasitology – Interpretation to Clinical medicine.
4. Rippon – Medical Mycology
5. Emmons – Medical mycology
6. Basic laboratory methods in Parasitology, 1st Ed, J P Bros, New Delhi – 199
7. Basic laboratory procedures in clinical bacteriology, 1st Ed, J P Brothers, New Delhi
 17

8. Medical Parasitology – Ajit Damle

9. Introduction to Medical Microbiology –Ananthanarayana, Orient Longman PVT Ltd.
Syllabus for First year BSc. Perfusion Technology
F BASICS IN COMPUTER APPLICATIONS

The course enables the students to understand the fundamentals of computer and its
applications.
Introduction to Data processing :
Features of computers, Advantages of using computers. Getting data into / out of
computers. Role of computers. What is Data processing? Application areas of computers involved in Data
processing. Common activities in processing. Types of Data processing,Characteristics of information.
What are Hardware and Software?Hardware Concepts :Architecture of computers, Classification of
computers, Concept of damage. Types of storage devices. Characteristics of disks, tapes, Terminals,
Printers, Network. Applications of networking concept of PC System care, Floppy care, Data care.
Concept of [Link] of software : System software. Application of software. Operating
system. Computer system. Computer virus. Precautions against viruses. Dealing withviruses. Computers in
medical electronicsBasic Anatomy of [Link] of programming
Computer application - principles in scientific research ; work processing, medicine,
libraries, museum , education, information system.
Data [Link] in physical therapy - principles in EMG, Exercise testing equipment, Laser.
Scheme of Examination for MEDICAL ELECTRONICS including COMPUTER
APPLICATIONS
One Written (Theory) paper: Maximum Marks: –80 marks.
No Practical or Viva voce examination

II Syllabus for Second year BSc. Perfusion Technology

A APPLIED PHARMACOLOGY

General concepts about pharmacodynamic and Pharmacokinetic Principles involved in drug activity.
I. Autonomic nerves system.
Anatomy & functional organisation. List of drugs acting an ANS including dose, route of administration,
indications,contra indications and adverse effects.
II. Cardiovascular drugs- Enumerate the mode of action, side
effects And therapeutic uses of the following drugs.
a. Antihypertensives
Beta Adrenergic [Link] Adrenergic [Link] [Link] channel
blockers
b. Antiarrhythmic drugs
c. Cardiac glycosides
d. Sympathetic and nonsympathetic inotropic agents.
e. Coronary vasodilators.
f. Antianginal and anti failure agents
g. Lipid lowering & anti atherosclerotic drugs.
h. Drugs used in Haemostais – anticoagulants Thrombolytics and antithrombolytics.
i. Cardioplegic drugs- History, Principles and types of cardioplagia.
j. Primary solutions – History, principles & types.
k. Drugs used in the treatment of shock.
III. Anaesthetic agents.
Definition of general and local [Link] of general anaesthetics.
 18

Pharmacokinetics and Pharmacodynamics of inhaled anaesthetic [Link] general anaesthetic

[Link] anaesthetics – classification mechanism of action, duration of action and
methods to prolong the duration of action. Preparation, dose and routes of administration.
IV Analgessics
Definition and [Link] of administration, dose, frequency of administration,Side effects and
management of non opioid and opiod analgesics
V. Antihistamines and antiemetics-
Classification, Mechanism of action, adverse effects,Preparations, dose and routes and administration.
VI. CNS stimulants and depressants
[Link], hypnotics and [Link] stimulants. Neuromuscular blocking agents and
muscle relaxants.
VII. Pharmacological protection of organs during CPB
VIII. Inhalational gases and emergency drugs.
IX. Pharmacotherapy of respiratory disorders
Introduction – Modulators of bronchial smooth muscle tone and pulmonary vascular smooth muscle tone
Pharmacotherapy of bronchial [Link] of cough. Mucokinetic and mucolytic agents
Use of bland aerosols in respiratory care.
X. Corticosteroids – Classification, mechanism of action, adverse effects and complications. Preparation,
dose and routes of administration.
XI Diuretics
Renal [Link] of action of [Link] [Link], dose and routes of
administrion.
XII. Chemotherapy of infections
Definition
Classification and mechanism of action of antimicrobial agents
Combination of antimicrobial agents
Chemoperophylaxis.
Classification, spectrum of activity, dose, routes of administration and adverse effects
of penicillin, cephalosporins, aminoglycosides, tetracyclines, chloramphenicol,
antitubercular drugs.
XIII. Miscellaneous.
IV fluids- various preparations and their usage.
Electrolyte supplements
Immunosuppressive agents
New drugs included in perfusion technology.
Drugs used in metabolic and electrolyte imbalance.
PRACTICALS:
1. Preparation and prescription of drugs of relevance.
2. Experimental pharmacology directed to show the effects of commonly used drugs
of relevance and interpretation of few charts.
Scheme of Examination
Theory
There shall be one theory paper of three hours duration carrying 80 marks. Distribution of type
of questions and marks for applied Pharmacology shall be as given under.

Type of Questions No. of Questions Marks Sub-total

Long Essay (LE) 3(To attempt 2) 2 x 10 20

30
8 (To attempt 6)
Short Essay (SE) 6x5
Short Answer (SA) 12 (To Attempt 10) 10 x 3 30
 19

Total Marks 80

NO PRACTICAL EXAMINATION
Recommended Books.
1. R. S. Satoskar, S.D. Bhandarkar, S. S. Ainapure, Pharmacology and Pharmacotherapeutics, 18th Edition,
single Volume, M/S Popular Prakashan,350, Madan Mohan Marg, Tardeo, Bombay – 400 034.
2. K.D. Tripathi, Essentials of Medical Pharmacology, V. Edition, M/s. Jaypee Brothers, Post Box, 7193,
G-16, EMCA House, 23/23, Bansari Road,Daryaganj, New Delhi.
3. Laurence and Bennet, Clinical Pharmacology, ELBS Edition, 9th Edition.
II Syllabus for Second year BSc. Perfusion Technology

B APPLIED PATHOLOGY

I. CARDIOVASCULAR SYSTEM
Atherosclerosis- Definition, risk factors, briefly Pathogenesis & morphology,clinical significance and
prevention.
Hypertension- Definition, types and briefly Pathogenesis and effects of Hypertension.
Aneurysms – Definition, classification, Pathology and complications.
Pathophysiology of Heart failure.
Cardiac hypertrophy – causes, Pathophysiology & Progression to Heart Failure.
Ischaemic heart diseases- Definition, Types. Briefly Pathophysiology,Pathology & Complications of
various types of IHD.
Valvular Heart diseases- causes, Pathology & complication. Complications of artificial valves.
Cardiomyopathy – Definition, Types, causes and significance.
Pericardial effusion- causes, effects and diagnosis.
Congenital heart diseases – Basic defect and effects of important types of congenital heart diseases.
II. HAEMATOLOGY
Anaemia – Definition, morphological types and diagnosis of [Link] concept about Haemolytic
anaemia and polycythaemia.
Leukocyte disorders- Briefly leukaemia, leukocytosis, agranulocytosis etc.,
Bleeding disorders- Definition, classification, causes & effects of important types of bleeding disorders.
Briefly various laboratory tests used to diagnose bleeding disorders.
III. RESPIRATORY SYSTEM
Chronic obstructive airway diseases – Definition and types. Briefly causes,Pathology and complications
of each type of COPD.
Briefly concept about obstructive versus restrictive pulmonary disease.
Pneumoconiosis- Definition, types, Pathology and effects in brief.
Pulmonary congestion and edema.
Pleural effusion – causes, effects and diagnosis.
IV. RENAL SYSTEM
Clinical manifestations of renal diseases. Briefly causes, mechanism, effects and laboratory diagnosis of
ARF & CRS. Briefly Glomerulonephritis and Pyelonephritis.
End stage renal disease – Definition, causes, effects and role of dialysis and renal transplantation in its
management.
Brief concept about obstructive uropathy.
PRACTICALS
1. Description & diagnosis of the following gross specimens.
a. Atherosclerosis.
b. Aortic aneurysm.
c. Myocardial infraction.
d. Emphysema
e. Chronic glomerulonephritis.
 20

f. Chronic pyelonephritis.
2. Interpretation & diagnosis of the following charts.
a. hematology Chart - AML, CML, Hemophilia, neutrophilia, eosinophilia.
b. Urine Chart - ARF, CRF, Acute glomerulonephritis.
3. Estimation of Hemoglobin.
4. Estimation Bleeding & Clotting time.
Scheme of Examination Theory
There shall be one theory paper of three hours duration carrying 50 marks. Distribution of type of questions
and marks for Applied Pathology shall be as given under

Type of Questions No. of Questions Marks Sub-total

Long Essay (LE) 3(To attempt 2) 2 x 10 20

30
8 (To attempt 6)
Short Essay (SE) 6x5
Short Answer (SA) 12 (To Attempt 10) 10 x 3 30
Total Marks 80

PRACTICAL EXAMINATION - 40 Marks.

There will be a Combined Practical examination for Applied Pathology & Applied Microbiology.

Sl. Tests Marks

No
01 Interpretation of Hematology Chart 05

02 Interpretation of Urine Chart 05

03 Estimation of Hemoglobin 05
04 Estimation of Bleeding time & Clotting time 05
Total 20

II Syllabus for Second year BSc. Perfusion Technology

C APPLIED MICROBIOLOGY
THEORY – 40 HOURS
1. Health care associated infections and Antimicrobial resistance: Infections that patients acquire during the
course of receiving treatment for other conditions within a healthcare setting like Methicillin Resistant
Staphylococcus aureus infections, Infections caused by Clostriduium difficle, Vancomycin resistant
enterococci etc. Catheter related blood stream infections, Ventilator associated pneumonia, Catheter
Related urinary tract infections, Surveillance of emerging resistance and changing flora. The impact and
cost attributed to Hospital Associated infection.6 Hours
2. Disease communicable to Healthcare workers in hospital set up and its preventive measure:
Occupationally acquired infections in healthcare professionals by respiratory route ( tuberculosis, varicella-
zoster, respiratory synctial virus etc ), blood borne transmission ( HIV, Hepatitis B, Hepatitis C,
Cytomegalovirus, Ebola virus etc), oro faecal route ( Salmonella, Hepatitis A etc), direct contact ( Herpes
Simplex Virus etc).Preventive measures to combat the spread of these infections by monitoring and
control. 6 Hours
 21

3. Microbiological surveillance and sampling: Required to determine the frequency of potential bacterial
pathogens including Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis and
also to assess the antimicrobial [Link]: rinse technique, direct surface agar plating technique.
6 Hours
4. Importance of sterilization:
a. Disinfection of instruments used in patient care: Classification, different methods, advantages and
disadvantages of the various methods.
b. Disinfection of the patient care unit
c. Infection control measures for ICU’s 10 Hours
5. Sterilization:
a. Rooms: Gaseous sterilization, one atmosphere uniform glow discharge plasma (OAUGDP).
b. Equipments: classification of the instruments and appropriate methods of sterilization.
c. Central supply department: the four areas and the floor plan for instrument cleaning, high-level
disinfecting and sterilizing areas. 8 Hours
6. Preparation of materials for autoclaving: Packing of different types of materials, loading, holding time
and unloading. 4 Hours
PRACTICALS- 30 HOURS
1. Principles of autoclaving & quality control of Sterilization.
2. Collection of specimen from outpatient units, inpatient units, minor operation theater and
major operation theater for sterility testing.
3. The various methods employed for sterility testing.
4. Interpretation of results of sterility testing.
5. Disinfection of wards, OT and Laboratory.
Scheme of Examination
Theory
There shall be one theory paper of three hours duration carrying 50 marks. Distribution of type
of questions and marks for Applied Microbiology shall be as given under.

Type of Questions No. of Questions Marks Sub-total

Long Essay (LE) 3(To attempt 2) 2 x 10 20

20
6 (To attempt 4)
Short Essay (SE) 4x5
Short Answer (SA) 7 (To Attempt 5) 5x2 10
Total Marks 50

PRACTICAL EXAMINATION - 40 Marks.

There will be a Combined Practical examination for Applied Pathology & Applied Microbiology.

Sl. Tests Marks

No
01 Dry heat / Moist heat: Temperature recording charts 05
interpretation

02 Dry heat / Moist heat: Color change indicators 05

interpretation

03 Air sampling culture plates interpretation of Colony 05

forming units based on air flow rate and sampling time
04 Interpretation of Sterility of Hemodialysis 05
 22

water/Distilled water /Deionised water based on growth

of colonies in BHI agar to be reported as X CFU/mL

Total 20

iiSyllabus for Second BSc. Perfusion Technology

D MEDICINE RELEVANT TO PERFUSION TECHNOLOGY

Cardiovascular System
Ischaemic heart [Link] heart [Link] heart [Link]
Aortic [Link] vascular [Link] edema and LV failure
Hematology
[Link] [Link] tests used to diagnose bleeding disorders (in brief)
Respiratory System
Chronic obstructive airway diseases (COPD).Concept of obstructive versus restrictive pulmonary disease
PFT and its interpretation
Renal System
ARF & [Link] stage renal [Link] of dialysis and renal transplantation in its management
CNS
Automatic nervous system.(Sympathetic & Parasympathetic system).Brief mention of CNS disorders &
their etiology
Others
[Link] Patient (neonate/Infant).Elderly patient
Scheme of Examination Theory

There shall be one theory paper of three hours duration carrying 80 marks. Distribution of type
of questions and marks for Medicine relevant to Perfusion Technology shall be as given
under.

Type of Questions No. of Questions Marks Sub-total

Long Essay (LE) 3(To attempt 2) 2 x 10 20

40
10 (To attempt8)
Short Essay (SE) 8x5
Short Answer (SA) 12 (To Attempt 10) 10 x2 20
Total Marks 80

NO PRACTICAL EXAMINATION
 23

II Syllabus for Second year BSc. Perfusion Technology

E INTRODUCTION TO PERFUSION TECHNOLOGY

Basics of diagnostic techniques:
Chest of [Link] [Link] investigations in relation to
perfusion [Link] bypass and perfusion technology
History of Cardiac surgery and [Link] reference of Gibbon Lillehei, carrel. Pre CPB surgery
Azygous Flow principle.
Hypothermic/nonhypothermic non-CPB surgery including gross’[Link] technique and controlled cross
circulation.
Monitoring and instrumentation
Concepts of monitoring – instrumentation technology of ECGmachine, pressure transducer, syringe and
peristaltic pumps,monitors, ventilators, pulse oximeters, temperature probes and thermo regulatory
monitoring, defibrillators and fibrillators. Piped and non-piped gas delivery systems and connections. Basic
physics related to medically used gases.
Haemodynamic monitoring
Haemostatic monitoring
Haemotologic monitoring
Maintenance of oxygen, carbon dioxide and acid-base status and their monitoring
Neurological monitoring (SSPE, EEG and cerebral function monitor)
Aseptic technique.
Cardiac surgery team, profession and terminology, scope of perfusion technology
Physiology of Extracorporeal circulation
Heart – Lung machine
Principles of extracorporeal circulation
Materials used in EC circuit
Principles of extracorporeal gas exchange
Various types of oxygenators
Bubble oxygenators
Rotating spiral/cylinder/disc oxygenators
Membrane oxygenators
Mechanism of action components defoaming, rated flow.
Theory of blood pumps
Ideal blood pump, pulsatile versus non-pulsatile flow, occlusive and non-occlusive pumps, various types
of pumps roller, bellow, sigmamotor, diaphragm, ventricular and centrifugal pumps.
Element of extracorporeal circulation/hazards of:
a. blood failure
b. Bubble trap
c. Flow meters
d. Temperatures
e. Heat exchanger
f. Regulating devices
Connection of the vascular system with extracorporeal circulation:
Arterial and venous cannulae.
Connecting tubes and connectors
Vents
Suckers
Cardioplegia delivery system
Venous drainage.
Haemodynamic of arterial return, venous drainage, cardioplegiaDelivery and [Link] banking,
handling of blood products and their management. Blood components and their use.
 24

Scheme of Examination Theory

There shall be one theory paper of three hours duration carrying 100 marks. Distribution of
type of questions and marks for Introduction to Anaesthesia Technology shall be as given
under.

Type of Questions No. of Questions Marks Sub-total

Long Essay (LE) 3(To attempt 2) 2 x 10 20

60
14 (To attempt8)
Short Essay (SE) 12 x 5
Short Answer (SA) 12 (To Attempt 10) 10 x2 20
Total Marks 100

PRACTICAL EXAMINATION - 40 Marks.

IIISyllabus for Third year [Link] Perfusion Technology
A Paper-I Perfusion Technology Clinical
1. Pharmacokinetics and Pharmacodynamics of Cardiopulmonary bypass
2. Drugs (including anesthetic drugs) used in cardiopulmonary bypass
3. Conduct and monitoring of Cardiopulmonary bypass
4. Adequacy of perfusion – General considerations, specific aspects of perfusion, monitoring, other
concomitants which may affect its adequacy
5. Pulsatile perfusion – Introduction, theory & physiology of pulsatile flow,hemodynamic, metabolic
effects, Clinical use, hematological effects
6. Cannulation techniques during cardaiopulmonary bypass
7. Termination of cardiopulmonary bypass – principles and methodology
8. Myocardial protection and cardioplegia- pretreatment of the Myocardium, cardioplegia, hypothermia,
controlled reperfusion, myocardial protection for specific clinical problems, Complications of cardioplegia.
Non cardioplegic methods during cardiac surgery on cardiopulmonary bypass
9. Oxygenation – general consideration, bubble & membrane (including assessment and comparison of
oxygenator function)
10. Heat exchangers-principles function of heat exchangers & their assessment. Complications related to
heat exchange and their management
11. Priming fluids and hemodilution
Scheme of Examination Theory
There shall be one theory paper of three hours duration carrying 100 marks. Distribution of
type of questions and marks for Paper-I - Perfusion Technology - Clinical shall be as given
under.

Type of Questions No. of Questions Marks Sub-total

Long Essay (LE) 3(To attempt 2) 2 x 10 20

60
14 (To attempt8)
Short Essay (SE) 12 x 5
Short Answer (SA) 12 (To Attempt 10) 10 x2 20
 25

Total Marks 100

PRACTICAL EXAMINATION
One common practical for all the three papers with equal weight age of marks i.e. 40 practical
marks for each paper.

III Syllabus for Third year [Link] Perfusion Technology

B Paper-II Perfusion Technology – Applied

1. Blood cell trauma – analysis of forces of fluid motion, effects of physical forces of blood cell, clinical
effect. Complications of blood transfusion.
2. Anticoagulation on bypass, its monitoring, its reversal and complications. Heparinless bypass. Platelet
aggregation and platelet dysfunction. Coagulopathies due to cardiopulmonary bypass and its management.
3. Inflammatory response to cardiopulmonary bypass & its clinical effects. Methods to minimise the same.
Immune response, neuroendocrine, renal, metabolic splanchnic response, pulmonary response and
electrolyte response to cardiopulmonary bypass
4. Blood conservation hemofiltration & dialysis during cardiopulmonary bypass including modified ultra
filtration reverse autologous priming and other methods
5. Micro emboli- gaseous and particulate, filters used in cardiopulmonary bypass circuit.
6. Micro pore filtration during cardiopulmonary bypass
7. Counter pulsation techniques and assist devices
Scheme of Examination Theory
There shall be one theory paper of three hours duration carrying 100 marks. Distribution of
type of questions and marks for Paper-II - Perfusion Technology Applied shall be as given
under.

Type of Questions No. of Questions Marks Sub-total

Long Essay (LE) 3(To attempt 2) 2 x 10 20

14 (To attempt8) 60
Short Essay (SE) 12 x 5
Short Answer (SA) 12 (To Attempt 10) 10 x2 20
Total Marks 100

PRACTICAL EXAMINATION
One common practical for all the three papers with equal weight age of marks i.e. 40 practical
marks for each paper.
III Syllabus for Third year [Link] Perfusion Technology
C Paper-III Perfusion Technology – Advanced

1. Perfusion techniques for Paediatric cardiac surgery

2. ECMO- special perfusion techniques for special cardiac surgeries and medical conditions (including
thoracic aortic surgeries deep hypothermia and circulatory arrest). Perfusion for non cardiac surgery,
invasive cardiology and outside the operation suite.
3. Perfusion as a method of cardiopulmonary bypass
4. Complications and safety during cardiopulmonary bypass – bypass safety, organizational aspects,
accidents, coagulpathies, mechanical and electrical failures, perfusion management, perfusion systems,
safety for the perfusionist and surgical team management of perfusion accidents.
 26

5. Minimally invasive surgery and the perfusionist

6. Recent advances in perfusion techniques
7. Experimental perfusion

Scheme of Examination Theory

There shall be one theory paper of three hours duration carrying 100 marks. Distribution of
type of questions and marks for Paper-III - Perfusion Technology Advanced shall be as
given under.

Type of Questions No. of Questions Marks Sub-total

Long Essay (LE) 3(To attempt 2) 2 x 10 20

60
14 (To attempt8)
Short Essay (SE) 12 x 5
Short Answer (SA) 12 (To Attempt 10) 10 x2 20
Total Marks 100

PRACTICAL EXAMINATION
One common practical for all the three papers with equal weight age of marks i.e. 40 practical
marks for each paper