The Laboratory - Grade 7 Integrated Science Revision Notes
The Laboratory - Grade 7 Integrated Science Revision Notes
The Laboratory
Laboratory safety
A place equipped for experimental study in a science or for testing and analysis. a research laboratory.
broadly: a place providing opportunity for experimentation, observation, or practice in a field of study.
Biohazard – It provides warning on lab equipment that may contain biohazardous materials like blood
samples.
1. CHEMICALS
If you handle chemicals, you need to wear protective gloves. Treat chemicals with the necessary
caution, measure chemicals carefully, contain potentially irritating or hazardous chemicals and use
only approved containers when you’re transferring chemicals.
Chemical burns should be treated by flushing the affected area with a large amount of cool,
running water for at least 15 minutes. If the burn area is large or if the affected person is dizzy or
weak, call 911.
If you work in a chemistry lab, ensure you are taking the proper steps to be safe around the
chemicals in your workplace.
2. HEAT
Exercise caution when handling hot items. Hastily or improperly handling these items without the
correct tools can cause serious burns. Workers should be properly using water baths, tongs and
cooling equipment. Be careful not to touch hot surfaces with your bare hand.
If you suffer a heat burn in the lab, run the burned area under cool water for five minutes.
3. CUTS AND SCRAPES
Using sharp tools in the laboratory setting can cause scrapes and cuts. Workers may also use
sharp objects, such as needles and razor blades, or need to clean up broken glass. To avoid cutting
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yourself on these items, carefully wrap them so that you can safely carry and dispose of them.
4. CONTAMINATION
Avoiding contamination is key to ensuring safety in the lab setting. Though the advice to wash
your hands may seem quite basic, it’s an important procedure to avoid contamination. After you
interact with any foreign substance, you should wash your hands thoroughly.
Protect your skin and clothing with the proper equipment, such as gloves, glasses and a lab coat.
You can contaminate areas outside of the lab if you carry bacteria or other substances on your
clothing or skin, which can cause the spread of illnesses and other dangers.
5. INHALING SUBSTANCES
Another common type of lab accident is inhalation. If you inhale chemicals or gases in a space that
isn’t properly ventilated, you may experience nausea, headaches or fainting. Ensure you follow
proper procedures for ventilation, such as using ventilation fans and measuring the amount of gas
emitted in a room.
6. FIRES
When working with hot surfaces and flammable materials, fires become a common danger. Review
and practice the proper procedures to ensure you minimize the risk of fire in the laboratory. All
flammable materials should be properly stored and sealed. Inspect burners for leaks to avoid
sudden flares.
7. SPILLS AND BREAKS
In the lab, glass beakers may be dropped and break. Liquids may be spilled. Generally, these
accidents are caused by rushing, being negligent and not properly following procedures. While you
work, move carefully through every step. Moving slowly but more steadily will be much better for
the safety of yourself and your colleagues.
Though students have a responsibility to themselves and their colleagues to exercise proper safety
measures and follow protocol, the laboratory environment itself should also follow guidelines from the
Occupational Safety and Health Administration (OSHA) to provide workers with safe working conditions.
Forcibly hold both eyes open under an emergency eyewash to ensure an effective wash behind
both eyelids.
If contact lenses are being worn, remove the contacts while flushing.
Continue flushing the eyes underneath the eyewash until emergency personnel arrive.
Report the injury to your principal investigator and/or laboratory/facility manager.
Wash affected area(s) with tepid water from an emergency safety shower. Take care not to break
skin.
Remove or cut off contaminated clothing while rinsing. Do not pull contaminated clothing over the
head.
For chemical and thermal burns, flush affected area(s) with water from the safety shower, if
indicated in safety data sheet.
For blood, biological, or radiological exposures use soap and water.
Keep flushing affected area(s) underneath the safety shower until emergency personnel arrive.
Report the injury to your principal investigator and/or laboratory/facility manager.
If exposed individual is unconscious, do NOT enter the lab if a possibility of oxygen depletion, toxic
vapors, or an explosive atmosphere exists.
If exposed individual is conscious, move the person to fresh, uncontaminated air.
Report the injury to your principal investigator and/or laboratory/facility manager.
Lab safety is important because it can prevent injury, keep us from making mistakes and save
lives.
Safety is important when working in the lab because it helps you avoid injury, keep yourself from making
mistakes and save lives of others who may be nearby when an accident happens, or if there is a fire or
other emergency situation that needs to be handled quickly and safely by someone who knows what
they're doing before the situation gets out of hand.
We should follow the lab safety protocols to stay safe and healthy!
By following the lab safety protocols, you will stay safe and healthy.
It is absolutely critical that you follow all the rules of your lab protocol when conducting
experiments in the lab. This allows you to avoid accidents and contamination, which can cause
injury or illness.
You should always wear protective clothing (goggles, gloves and a face mask) to keep yourself
safe from hazardous materials such as chemicals or glass shards if an accident occurs in the
laboratory.
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You should never eat or drink anything in the laboratory because it could easily contaminate your
food with chemicals or bacteria potentially harmful for consumption by humans!
1. Observing
This is the most basic skill in science. Observations are made by using the 5 senses. Good
observations are essential in learning the other science process skills.
Examples:
The learner will be blind folded and make observations using the sense of touch.
The learner will create observations about a coin using the 5 senses.
The learner will mix primary colors to create more colors.
2. Communicating
It is important to be able to share our experiences. This can be done with graphs, diagrams, maps,
and spoken word.
Examples:
The learner will create a line graph showing the relationship between speed and the mass of a
marble.
The learner will discuss possible errors with other classmates.
3. Classifying
After making observations it is important to notice similarities, differences, and group objects
according to a purpose. It is important to create order to help comprehend the number of objects,
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SI Units
The International System Of Units (SI) is the metric system that is used universally as a standard for
measurements. SI units play a vital role in scientific and technological research and development.
SI unit is an international system of measurements that are used universally in technical and scientific
research to avoid the confusion with the units. Having a standard unit system is important because it
helps the entire world to understand the measurements in one set of unit systems. Following is the table
with base SI units:
There are 7 SI base units. The seven units along with their SI unit and symbol are given below:
1. Unit of length, meter (m): Meter is the SI unit of length and is defined by taking the fixed value of
the speed of light in vacuum. It is expressed as m.s-1.
2. Unit of mass, kilogram (kg): Kilogram is the SI unit of mass and is defined by taking the fixed value
of the Planck constant. It is expressed as kg
3. Unit of time, second (s): Second is the SI unit of time and is defined by taking the fixed value of
Cesium frequency.
4. Unit of electric current, ampere (A): Ampere is the SI unit of electric current and is defined by
taking the fixed value of the elementary charge.
5. Unit of thermodynamic temperature, Kelvin (K): Kelvin is the SI unit of thermodynamic
temperature
6. Unit of the amount of substance, mole (mol): Mole is the SI unit of the amount of substance and is
defined by the fixed value of Avogadro constant NA.
7. Unit of luminous intensity, candela (cd): Candela is the SI unit of luminous intensity and is defined
by the fixed value of the luminous efficacy.
Laboratory apparatus
An apparator /apparatus are scientific tools/equipment used in performing scientific experiments. The
conventional apparator used in performing scientific experiments is called standard apparator/apparatus.
If the conventional standard apparator/apparatus is not available, an improvised apparator/apparatus may
be used in performing scientific experiments. An improvised apparator/apparatus is one used in
performing a scientific experiment for a standard apparator/apparatus. Most standard apparatus in a
school chemistry laboratory are made of glass because:
i. Glass is transparent and thus reactions /interactions inside are clearly visible from outside
ii. Glass is comparatively cheaper which reduces cost of equipping the school chemistry laboratory
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Apparatus are designed for the purpose they are intended in a school chemistry laboratory:
A Bunsen burner uses butane/laboratory gas as the fuel. The butane/laboratory gas
is highly flammable and thus usually stored safely in a secure chamber outside
Chemistry school laboratory. It is tapped and distributed into the laboratory through
gas pipes.
The gas pipes end at the gas tap on a chemistry laboratory bench .If opened the
gas tap releases butane/laboratory gas. Butane/laboratory gas has a characteristic
odor/smell that alerts leakages/open gas tap.
The Bunsen burner is fixed to the gas tap using a strong rubber tube.
The Bunsen burner is made up of the following parts:
i. Base plate –to ensure the burner can stand on its own
ii. Jet-a hole through which laboratory gas enters the burner
iii. Collar/sleeve-adjustable circular metal attached to the main chimney/burell
with a side hole/entry. It controls the amount of air entering used during
burning.
iv. Air hole- a hole/entry formed when the collar side hole is in line with
chimney side hole. If the collar side hole is not in line with chimney side
hole, the air hole is said to be “closed” If the collar side hole is in line with
chimney side hole, the air hole is said to be “open”
v. Chimney- tall round metallic rod attached to the base plate.
b. Procedure for lighting/igniting a Bunsen burner
a. Adjust the collar to ensure the air holes are closed.
b. Connect the burner to the gas tap using a rubber tubing. Ensure the rubber
tubing has no side leaks.
c. Turn on the gas tap.
d. Ignite the top of the chimney using a lighted match stick/gas lighter/wooden
splint.
e. Do not delay excessively procedure (iv) from (iii) to prevent highly
flammable laboratory gas from escaping/leaking.
A compound microscope is a high-power microscope that has higher magnification levels than a low-power
or dissection microscope. It is used to examine tiny specimens like cell structures that cannot be viewed at
lower magnification levels. A compound microscope is made up of both structural and optical components.
The 3 basic structural components are – the head, arm and base.
The body or head comprises the optical parts present in the upper part of the microscope
The arm connects and supports the base and head of the microscope. Also, it is used to carry the
microscope.
Base of the microscope supports the microscope and comprises the illuminator
Eyepiece
Eye tube
Objective lenses
Nosepiece
Adjustment knobs
Stage
Illuminator
Condenser and condenser focus knob
Diaphragm
The ocular or eyepiece is what an observer looks through and is present in the upper portion of the
microscope. The eyepiece tube clasps the eyepieces which are positioned above the objective lens. The
objective lenses are the main optical lenses. They range in various magnifications from 4x to 100x and
generally include 3 to 5 lenses on a single microscope. Nosepiece houses the objective lenses.
The fine and coarse focus knobs are the adjustment knobs that are often used to focus the microscope.
They are coaxial knobs. This means the focusing system of both fine and coarse focus are mounted on the
same axis. There is also a condenser focus knob which moves the condenser up or down to control the
lighting
The stage is where the specimen to be viewed is placed. A mechanical stage is often used when working
on a specimen at a higher magnification. This is when delicate movement of the specimen is required.
Stage clips are operated to hold the slide in place. To see different areas of the specimen, the observer
must physically move the slide. A separate knob is present to move the slide in the mechanical stage. The
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aperture is a tiny hole in the stage via which the transmitted light enters the stage.
An illuminator acts as the light source and is typically located at the microscope’s base. Most light
microscopes operate on halogen bulbs with low voltage and also have variable and continuous lighting
control within the base. A condenser is typically used to gather and focus the illuminator’s light onto the
specimen. It is found beneath the stage and is often observed in conjunction with a diaphragm or iris. Iris
or Diaphragm regulates the amount of light that reaches the specimen. It is situated above the condenser
but beneath the stage.
Functions of Microscope
The primary function of a microscope is to study biological specimens. A microscope solely functions on
two concepts – magnification and resolution. Magnification is simply the ability of the microscope to
enlarge the image. Whereas the ability to analyse minute details depends on the resolution.
Compound and dissection microscopes are the two types of microscopes that are mostly used in schools
for educational purposes.