Phosphorous UV fluid Mono
Colorimetric determination of inorganic Phosphorous in serum and plasma with the mono reagent
REAGENT PACKAGE INFORMATION INTERFERENCES
Product FURUNO Configuration Free type of Bilirubin:
Product No. A little decrease, approx.0.45mg/dL by 10mg free type of bilirubin
Name Analyzer R1 R2
PF01917067-F5 5 x 67mL - Conjugate type of bilirubin:
Phosphorous CA-270 A little decrease, approx.0.69mg/dL by 10mg of conjugate type of bilirubin
PF01917067-F 10 x 67mL -
UV fluid CA-400 Hemolytic hemoglobin
PF01917067-F4 4 x 20mL -
Mono CA-800 Decrease, approx. 1.25mg/dL per 100mg/dL of Hb
PF01917067-F16 16 x 20mL -
Chyle: No interference less than 1800 FTU (*)
*: FTU: Formazine turbidity unit
INTENDED USE
In vitro test for the quantitative determination of inorganic Phosphorous in
human serum, plasma and urine.
TESTING PROCEDURE
Material provided
- Working solutions as described above
SUMMARY Additional materials required
88% of the phosphorous contained in the body is localized in bone in the form - Calibrators and controls as indicated below
of calcium phosphate as the apatite. The remainder is involved in intermediary - “Saline Diluent” is used as diluent:
carbohydrate metabolism and in physiologically important substances such as
Product No. Product name Configuration
phospholipids, nucleic acids and ATP Phosphorus occurs in blood in the form of
FSD917070 Saline Diluent 5 x 67mL
inorganic phosphate and in organically bound phosphoric acid. The small
amount of extracellular organic phosphorus is found almost exclusively in the
form of phospholipids. MESUREMENT RANGE
The ratio of phosphate to calcium in the blood is approximately 6:10. An CA-270 0.19 – 30 mg/dL
increase in the level of phosphorus causes a decrease in the calcium level. The CA-400 0.19 – 30 mg/dL
mechanism is influenced by interactions between parathormone and vitamin D. CA-800 0.19 – 30 mg/dL
Hypoparathyroidism, vitamin D intoxication and renal failure with decreased
glomerular phosphate filtration give rise to hyperphosphatemia. CONVERSION FACTOR
Hypophosphatemia occurs in rickets, hyperparathyroidism and Fanconi's mg/dL mmol/L Factor: 0.323
syndrome. The preferred method for the determination of inorganic phosphorus mmol/L mg/dL Factor: 3.097
is based on the formation of ammonium phosphomolybdate with subsequent
reduction to molybdenum blue. Reagent stability problems often occur with this EXPECTED VALUES
method. The method presented here is based on the reaction of phosphate with Reference: Tietz et. al.
ammonium molybdate to form ammonium phosphomolybdate without reduction. mg/dL mmol/L
The addition of an accelerator gives rise to a more rapid rate of reaction and the Serum, Child 4.0 – 7.0 1.29 – 2.26
application of sample blanking yields more precise results. Plasma
Adult 2.5 – 4.5 0.81 – 1.45
MEASUREMENT PRINCIPLE g/day mmol/day
In sulphuric acid solution inorganic phosphate forms an Urine, 24hrs Adults 0.4-1.3 12.9-42.0
ammoniumphosphomolybdate-complex with the formula (NH4)3[PO4(MoO3)12]. Each laboratory should investigate the transferability of the expected values to
The increase in absorbance is measured at 334/340 nm and is proportional to its own patient population and if necessary determine its own reference range.
the inorganic phosphorous concentration. For diagnostic purposes, the phosphorus results should always be assessed in
Due to presence of detergents there is no need for deproteinization. conjunction with the patient's medical history, clinical examinations and other
findings.
CONCENTRATION OF WORKING SOLUTION
Monoreagent REPRODUCIBILITY
Sulphuric acid 280 mmol/L Reproducibility in CA-800
Sodium chloride 150 mmol/L Day-to-day run
Ammonium molybdate 0.9 mmol/L Within run (n= 20)
Detergents 1% (Duplicate, 20days)
Mean SD Mean SD
CV% CV%
REAGENT PREPARATION, STORAGE AND STABILITY (mg/dL) (mg/dL) (mg/dL) (mg/dL)
R1: Ready to use Low 3.80 0.028 0.73 3.93 0.0529 1.35
The sealed reagents are stable up to the indicated expiry date if stored at +18° - High 7.14 0.046 0.64 7.80 0.0667 0.85
+22°C. After opening the bottle, it is stable for 56 days when kept onboard in
the analyzer, if contamination is avoided. TEST COMPARISON
Comparison with a test of other manufacturer’s reagent of phosphorous
SAMPLE MATERIAL reagent in CA-800
Serum, plasma, urine (dilute 1+20 with saline) Y = 1.083 x - 0.123, R = 0.999, N = 110
Stability: 1 day at +15°C to +25°C
4 days at + 2°C to + 8°C QUALITY CONTROL
1 year at -20°C Multi QC sample “Centronorm” and ” Centropath” are used.
Centrifuge samples containing precipitate before performing the assay. Product No. Product Name Configuration
Strong lipemic and hemolytic sera should not be used. Contaminated
CNORM005 5 x 5 mL
glassware is the greatest source of error. Disposable plastic ware is
CNORM010 Centronorm 10 x 5 mL
recommended for the test.
CNORM020 20 x 5 mL
CPATH005 5 x 5 mL
PRECAUTIONS AND WARNINGS
For in vitro diagnostic use only. Attend to the normal precautions required for CPATH010 Centropath 10 x 5 mL
handling all laboratory reagents. CPATH020 20 x 5 mL
Attention: The reagent sulfuric acid. Corrosive. In the case of contact rinse with The control intervals and limits must be adapted to the individual laboratory and
plenty of water. Get immediate medical attention, if the reagent comes into country-specific requirements. Values obtained should fall within established
contact with eyes or if ingested. limits.
Each laboratory should establish corrective measures to be taken if values fall
outside the limits.
Centronic GmbH
Am Kleinfeld 11, 85456 Wartenberg/Germany
Phone: 0049-8762724300, Fax: 0049-8762724312
Edition 06/2021, TV-066-CE-003FU
e-mail: [email protected]
web: www.centronic-gmbh.com
CALIBRATION
Multi-calibrator “Centrocal” is used.
S1: Saline, S2: Centrocal
Recommended calibration interval: Weekly
Product No. Product Name Configuration
CCAL005 5 x 3mL
CCAL010 Centrocal 10 x 3mL
CCAL020 20 x 3mL
LITERATURE
Henry, R.J.: Clin.Chem., Harper&Row Publishers, NY (1974)
Taussky, H.H., Schorr, E.: J. Biol. Chem. 202, (1953), 675
Thomas, L.: Labor und Diagnose, 2. Aufl., Med. Verl. Marburg (1979)
Tietz, N.: Clinical Guide to Laboratory Tests, W. B.: Saunders Company
Philadelphia (1983), 384
Carl A. Burtis, David E. Bruns: Tietz fundamentals of clinical chemistry and
molecular diagnostics, 7th ed.
Centronic GmbH
Am Kleinfeld 11, 85456 Wartenberg/Germany
Phone: 0049-8762724300, Fax: 0049-8762724312
Edition 06/2021, TV-066-CE-003FU
e-mail: [email protected]
web: www.centronic-gmbh.com
