1. Which of the following factors render culture based methods less reliable ?
a. Transport the specimens under less than ideal conditions
b. Prior use of antibiotics
c. Small number of organisms
d. All of the above
2. The purpose and philosophy of diagnostic bacteriology depend on:
a. Identifying the causative pathogen by a different lab method
b. Identifying the causative pathogen by the same lab method
c. Both a and b
d. None of the above
3. Which of the following basic lab techniques is considered a definitive microbiological
diagnosis of an infectious disease?
a. Morphological identification
b. Cultivation and identification
c. Detection of microbial DNA & RNA
d. Detection of the host's immune response to the pathogen
e. All of the above
4. Many factors limit the success of sterilization, disinfection, or decontamination, such as
organic load (organism, blood, or body fluid):
a. Organic load (organism, blood, or body fluid)
b. Type of organisms present
c. The concentration and exposure time to germicide
d. The physical and chemical nature of the surface, temp, pH, humidity
e. All of the above
5. Manufacturer's Material Safety Data Sheet (MSDS) indicates what type of lab safety?
a. fire safety
b. Electrical safety
c. biosafety
d. Chemical safety
e. all of the above
6. The process that kills all forms of microbial life, including bacterial endospores, is called:
a. Disinfection
b. Decontamination
c. Sterilization
d. Biosafety
e. None of the above
7. Physical methods of sterilization include:
a. Incineration
b. Moist heat
c. Dry heat
d. Ionizing (gamma radiation)
e. All of the above
8. Many fastidious microorganisms, such as... die during transport to the lab:
a. Neisseria gonorrhoeae
b. E. coli
c. Staphylococcus aureus
d. Haemophilus influenzae
e. All of the above
9. Non-culture techniques include:
a. Direct microscopy
b. Immunological methods
c. Serology
d. PCR
e. All of the above
10. Bile salts and crystal violet are used to suppress the growth of most:
a. Fastidious microorganisms
b. Gram-positive bacteria
c. Gram-negative bacteria
d. Both a and b
e. None of the above
11. MacConkey agar is used for the detection of:
a. Salts tolerant bacteria
b. Lactose fermenting bacteria
c. Lactose fermenting yeast
d. All of the above
e. None of the above
12. Mannitol salt agar is used for the detection of:
a. E. coli
b. staphylococus aureus
c. Salmonella spp.
d. All of the above
e. None of the above
13. When the temperature of molten agar comes down to... it is poured into petri dishes:
a. 20°C
b. 37°C
c. 45°C
d. 47°C
e. 25°C
14. India ink or nigrosin is classified as:
a. Simple stain
b. Negative stain
c. Differential stain
d. Acid-fast stain
e. Both a and b
15. H2O + O2 is released from H2O2 by the action of which enzyme?
a. Catalase
b. Oxidase
c. Urease
d. Indole test
e. Decarboxylation
16. Which of the following tests guides the clinician in selecting the best antibiotic for a
patient?
a. Anticoagulation
b. Antibody-antigen reaction
c. Hemagglutination test
d. Antimicrobial susceptibility test
e. All of the above
17. Both pneumococci and Neisseria meningitidis are:
a. Gram-negative
b. Bacilli
c. Diplococci
d. All of the above
e. None of the above
18. Streptococcus pyogenes growing on a blood agar plate shows which of the following?
a. Alpha hemolysis
b. Alpha hemolysis, green zone surrounding the colonies
c. Beta hemolysis
d. Beta hemolysis, a green zone surrounding the colonies
e. Gama hemolysis
19. The best clinical specimen used for the isolation of bacterial meningitis is:
a. Urine
b. Blood
c. CSF
d. Sputum
e. None of the above
20. Which of the following methods is most specific and sensitive for the diagnosis of viral
infection?
a. Molecular method
b. Culturing method
c. Staining method
d. Wet mount method
e. Both b and d
1. D -medium
2. A- medium
3. E -hard
4. E- EASY
5. D -EASY
6. C- medium
7. E- medium
8. A- medium
9. E- medium
10. B -medium
11. B- medium
12. B- EASY
13. C- EASY
14. B- EASY
15. A- medium
16. D- medium
17. C- medium
18. C- medium
19. C- medium
20. A- EASY
