Contents

1. About this edition of operating manual .....................................................................2

2. Safety precautions ...................................................................................................2
3. General information ..................................................................................................4
4. Getting started..........................................................................................................4
5. Operation ................................................................................................................. 6
6. Specifications ...........................................................................................................8
7. Maintenance............................................................................................................. 9
8. Warranty................................................................................................................. 10
9. EU Declaration of Conformity ................................................................................. 11

1. About this edition of operating manual

The manual applies to following version of High-speed Mini-centrifuge:

 Microspin 12 version V.3AY

2. Safety precautions

Caution! Make sure you have fully read and understood the present manual
before using the equipment. Please pay special attention to sections
marked by this symbol.
GENERAL SAFETY
 The protection provided can be ineffective if the operation of the appliance does not
comply with the manufacturer's requirements.
 Save the unit from shocks or falling.
 Store and transport the unit at ambient temperatures between -20°C and +60°C and
maximum relative humidity of 80%.
 After transportation or storage keep the unit under room temperature for 2-3 hrs
before connecting it to the mains.
 Before using any cleaning or decontamination methods except those recommended
by the manufacturer, check with the manufacturer that the proposed method will not
damage the equipment.
 Do not make modifications in design of the unit.

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ELECTRICAL SAFETY
 Connect only to the external power supply with voltage corresponding to that on the
serial number label.
 Use only the external power supply provided with this product.
 Ensure that the external power supply is easily accessible during use.
 Disconnect the unit from the mains before moving.
 Disconnect the external power supply from power socket to turn off the unit.
 If liquid penetrates into the unit, disconnect it from the external power supply and
have it checked by a repair and maintenance technician.
 Do not operate the unit in premises where condensation can form. Operating
conditions of the unit are defined in the Specifications section.
DURING OPERATION
 Do not operate the unit without rotor protection lid.
 Do not operate the unit in environments with aggressive or explosive chemical
mixtures. Please contact manufacturer for possible operation of the unit in specific
atmospheres.
 Do not use rotor or adapters with visible signs of corrosion, wear or mechanical
damage.
 Do not operate the unit if it is faulty or has been installed incorrectly.
 Do not use outside laboratory rooms.
 Ensure that no persons and/or dangerous materials are located within a safety zone
of 300 mm around the equipment when the centrifuge is running.
 Do not centrifuge flammable or chemically vigorously reactive materials.
 Do not fill in the tubes after they are inserted in the rotor.
 Do not fill centrifuge rotor over the capacity specified by the manufacturer (see the
Specifications section).
 Use only original accessories provided by the manufacturer.
 Rotor must always be fixed securely. Stop the operation immediately by pressing the
Run Stop button if any unusual noise occurs during acceleration, which can be due
to improper rotor fixation.
BIOLOGICAL SAFETY
 It is the user’s responsibility to carry out appropriate decontamination if hazardous
material is spilt on or penetrates into the equipment.

3
3. General information

Microspin 12 high-speed mini-centrifuge is nice example of a good quality instrument

for components separation, which can be used for extracting RNA/DNA samples, separation
of cell suspensions and for the others micro quantitative analyses.
Microspin 12 has a spherical bioform shape and its compact footprint requires limited
space on the laboratory bench. Centrifuge has a single, aluminium fixed rotor, which spins
up to 14500 rpm, that is approximately 12400g. The rotor can accommodate up to 12 x 2 ml
(or smaller) microtubes (such as Eppendorf, Axygen, etc.). Adapters for 0.5 ml and 0.2 ml
microtubes are included in the standard set. The enlarged lid for the rotor, MSL-SC, allows
centrifuging spin columns.
Microspin 12 is equipped with an efficient fan system, which provides constant air-
cooling for the rotor to reduce the risk of sample overheating during operation. There is only
a slight sample temperature elevation during longer centrifugation periods (e.g. 10°C after
20 min. at maximal rotation speed).
Microprocessor control provides precise control of the set and actual parameters and
user-friendly interface with straightforward set-up. LCD screen indicates two lines of set and
actual values:
- centrifugation time;
- centrifugation speed;
- relative centrifugal force.
Brushless motor provides quiet vibration free performance even at high speeds and
long product service life. Metal protective inserts and enclosures inside the body and lid of
the centrifuge as well as automatic imbalance switch-off and lid locking mechanism provide
safe operation throughout the speed range. Sound signal indicates when centrifugation is
completed.
Due to its external power supply, Microspin 12 can be safely used in cold rooms (from
+4 °C to 15 °C).

4. Getting started

4.1. Unpacking. Remove packing materials carefully and retain them for future shipment
or storage of the unit. Examine the unit carefully for any damage incurred during
transit. The warranty does not cover in-transit damage. Warranty covers only the units
transported in the original package.
4.2. Complete set. Package contents:
- Microspin 12 High-speed Mini-centrifuge .......................................................... 1 pce
- MSR-12 rotor  with MSL-SC lid  and securing nut ...................................... 1 pce
- A-05 adapters for 0,5 ml tubes  .................................................................... 12 pcs
- A-02 adapters for 0,2 ml tubes  .................................................................... 12 pcs
- pin for the lid unblocking (screwed in rear panel of the unit)  ......................... 1 pce
- wrench for rotor removing  ............................................................................. 1 pce
- external power supply ....................................................................................... 1 pce
- power cord ........................................................................................................ 1 pce
- Operating manual, Certificate ......................................................................... 1 copy

4
 




 
4.3. Setup.
- Place the centrifuge on even stable surface.
- Remove protective film from the display.
- Connect the power cord to the external power supply.
- Plug the power cord into the socket on the rear, and position the centrifuge so that
there is easy access to the power switch and connector.
- According to en 61010-2-20, people and hazardous materials must not be within a
300 mm area around the device during the centrifuge operation.
- Do not place any objects in front of the ventilation slots underneath and 100 mm
behind the centrifuge.
4.4. Rotor and adapter installation:
- Connect the centrifuge to a properly grounded power socket. Switch on the power
switch (position I) on the rear.
- Press the Open key (fig. 3/1) and open the outer lid lifting it upwards by hand.
- Unscrew a fixation nut counter clockwise using the wrench included in standard set
and remove it.
- Place the rotor (fig. 1/1) and secure it tightly with the fixation nut, placing the nut
with the key holes up (fig. 1/2) and turning it clockwise by securing wrench.
- Insert adapters in the rotor sockets if it is necessary.
- Place the rotor lid on the rotor pressing the lid holder down tightly (fig. 2/1).
- Close the outer lid.
- Turn off the centrifuge with switch on the rear (position O).
≤ 8 mm
≤ 47 mm

Figure 1. Installing the rotor Figure 2. Installing the rotor lid Figure 3.

5
 - The height of the spin column must be 47 mm or less, and the portion above the
rotor must be 8 mm or less, see figure 3 for explanation.
- When loading spin columns, ensure that spin column hinges are not pointed
outwards. Otherwise, rotor lid cannot close properly. See figure 4 for explanation.

Wrong Correct Correct

Figure 4. Loading spin columns

5. Operation

Recommendations during operation

Use even number of tubes arranged symmetrically (one opposite another) when
loading to give the unit even balance during operation. The opposite tubes must be
filled up equally.
 Recommended time interval between operation sessions:
- for 15 min operation session – 10 min,
- for 30 min operation session – 15 min.
5.1. Check the external power supply power cord for any signs of damage preliminary and
replace if necessary. Connect the power cord to a properly grounded power socket.
Set the power switch on the rear side to I position (ON).
5.2. The centrifuge switches on and the following readouts show on the display:
- Previously set time, speed and relative centrifugal force, accordingly the set speed
in the upper line (Set).
- Mode indication (STOP – means that the lid is closed and the rotor is stopped) and
current speed - 0 rpm and correspond centrifugal force in the lower line (Actual).
5.3. Press the Open key (fig. 5/1) and open the outer lid by lifting it upwards by hand. The
display shows OPEN (see figure 5). The lid can be opened only when the rotor is
stopped.
5.4. Remove the rotor lid lifting the lid holder up.
5.5. Check the rotor for any signs of wear and replace if necessary. Insert EVEN number
of tubes in rotor one opposite another. The opposite tubes must be filled equally.
Note! When loading spin columns, ensure that spin column hinges are not
pointed outwards. Otherwise, rotor lid cannot close properly. See
figure 4 for explanation.

6
 Figure 6. Display of the stopped centrifuge

Figure 7. Display of the working centrifuge

Figure 5. Control panel
5.6. Place the rotor lid on the rotor pressing the lid holder down tightly. Close the outer lid
(the clicking sound of the lock and readings STOP in the lower line of the display
indicate that the lid is closed, see fig. 6).
5.7. Use the ▲ and ▼ Time keys (fig. 5/2) to set the required time interval.
5.8. Use the ▲ and ▼ Speed keys (fig. 5/3) to set the required speed and required
centrifugal force, using the centrifugal force readings (fig. 5/5). These parameters can
also be adjusted during operation.
Note! Some plastic tubes can be damaged at higher speeds. Refer to the
tube material specifications to make sure that it will not get damaged
at the set speed.
5.9. Press the Run Stop key (fig. 5/4) to start centrifugation. Blinking RUN indication and
current speed is displayed in the lower line (fig. 7). The timer in the upper line starts
countdown after the set speed is achieved (stable RUN indication).
Note! If the rotor imbalance occurs causing vibration the centrifuge will stop
automatically (IMBALANCE indication will be shown). After the rotor
is stopped, open the lid and remedy the cause of imbalance.
5.10. Centrifugation is stopped automatically after the set time elapses (while braking
display shows blinking indication STOP (fig. 6). A sound signal is emitted after full
stop of the rotor (press the Run Stop key (fig.3/4) to stop the signal).
5.11. Centrifugation can be stopped before the set time elapses if necessary by pressing
the Run Stop key. The set time interval will be shown on the display.
5.12. After finishing the operation, turn off the centrifuge with switch (O position) on the
rear. Disconnect the device from the mains.
Note! The electrical lid lock allows opening the lid only when the unit is
connected to the mains and is turned on. Do not force the lid to open
when the unit is switched off!
5.13. Emergency opening. Disconnect the power cord from the mains. Wait until the
centrifuge full stopping. Find the emergency opening slot on the right side of the unit.
Insert the pin for unblocking the lid (screwed in rear panel of the unit) into the
emergency opening slot and press until the lid opens.

7
6. Specifications

The unit is designed for operation in cold rooms, incubators (excluding CO2
incubators) and closed laboratory rooms at ambient temperature from +4°C to +40°C in a
non-condensing atmosphere and maximum relative humidity 80% for temperatures up to
31°C decreasing linearly to 50% relative humidity at 40°C.
Biosan is committed to a continuous programme of improvement and reserves the
right to alter design and specifications of the equipment without additional notice.
5.1. Rotor imbalance automatic diagnostics ..... emergency stop, IMBALANCE indication
5.2. Speed control range ......................................................................1000 – 14500 rpm
5.3. Speed setting resolution ................................................................................100 rpm
5.4. Relative centrifugal force control range .............................................. 50 – 12400 x g
5.5. Digital time setting ................................................................................. 15s – 30 min
5.6. Time setting resolution
Shorter than 1 min ......................................................................................... 15 s
1 min and longer .......................................................................................... 1 min
5.7. Acceleration time up to 14500 rpm1 .................................................................... 20 s
5.8. Slowdown time, not more .................................................................................... 10 s
5.9. Display ............................................................................................................... LCD
5.10. Standard MSR-12 rotor capacity ........................................... for 12 х 1.5/2 ml tubes
5.11. Maximum tube height
Above the rotor ......................................................................................... ≤ 8 mm
Full tube .................................................................................................. ≤ 47 mm
5.12. Maximum rotor load ............................................................................................ 36 g
5.13. Dimensions .................................................................................. 200x240x125 mm
5.14. Input current/power consumption .................................................... 24 V, 2,5 A/60 W
5.15. External power supply ..................... input AC 100–240 V 50/60 Hz, output DC 24 V
5.16. Weight2............................................................................................................. 3.5 kg

Replacement parts Description Catalogue number

A-05 Adapter for 12x0.5 ml microtubes BS-010213-AK
A-02 Adapter for 12x0.2 ml microtubes BS-010213-BK
higher rotor lid to accommodate spin
MSL-SC BS-010213-EK
columns

1
Accurate within ± 8 s
2
Accurate within ± 10%
8
7. Maintenance

7.1. If the unit requires maintenance, disconnect the unit from the mains and contact
Biosan or your local Biosan representative.
7.2. All maintenance and repair operations must be performed only by qualified and
specially trained personnel.
7.3. Standard ethanol (75%) or other cleaning agents recommended for cleaning of
laboratory equipment can be used for cleaning and disinfection of the unit.
7.3.1. For rotor cleaning, it is necessary to do the following operations:
- If the centrifuge is switched on, press the Open key (fig.1/1) and open the outer lid
lifting it upwards with a hand.
- If the centrifuge is switched off the mains, find the emergency opening slot on the
right side of the unit. Insert the pin for unblocking the lid (screwed in rear panel of
the unit) into the emergency opening slot and press until the lid opens.
- Remove the rotor lid lifting the lid holder up.
- Hold the rotor with one hand and turn a fixation nut counter-clockwise to release the
rotor with the help of wrench included in standard set.
- Release the rotor and clean it.
- The rotor is autoclavable (without the lid, 120°С, 20 min).
Note! Due to frequent autoclaving the rotor label can be damaged or
unstuck. If necessary, a new label can be requested from the
manufacturer or your local distributor.
- After cleaning install the rotor, secure it carefully turning the rotor fixation nut tightly.
- Place the rotor lid on the rotor pressing the lid holder down. Close the outer lid.

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8. Warranty

8.1. The Manufacturer guarantees the compliance of unit with the requirements of
Specifications, provided the Customer follows the operation, storage and
transportation instructions.
8.2. The warranted service life of unit from date of delivery to the Customer is 24 months.
For extended warranty, see 8.5.
8.3. Warranty covers only the units transported in the original package.
8.4. If any manufacturing defects are discovered by the Customer, an unsatisfactory
equipment claim shall be compiled, certified and sent to the local distributor address.
To obtain the claim form, visit section Technical support on our website at link below.
8.5. Extended warranty. For Microspin 12, a Basic Plus class model, extended warranty
is a paid service. Contact your local Biosan representative or our service department
through the Technical support section on our website at the link.
8.6. Description of the classes of our products is available in the Product class
description section on our website at the link below.
Technical support Product class description

biosan.lv/en/support biosan.lv/classes-en
8.7. The following information will be required in the event that warranty or post-warranty
service comes necessary. Complete the table below and retain for your records.

Model Microspin 12, High-speed Mini-centrifuge

Serial number

Date of sale

10
9. EU Declaration of Conformity

Unit type Mini-centrifuge, laboratory centrifuges

Models Microspin 12, LMC-3000, LMC-4200R
Serial number 14 digits styled XXXXXXYYMMZZZZ, where XXXXXX is
model code, YY and MM – year and month of production,
ZZZZ – unit number.
Manufacturer SIA BIOSAN
Latvia, LV-1067, Riga, Ratsupites str. 7/2
Applicable Directives EMC Directive 2014/30/EU
LVD Directive 2014/35/EU
RoHS2 2011/65/EU
WEEE 2012/19/EU
Applicable Standards LVS EN 61326-1: 2013
Electrical equipment for measurement, control and
laboratory use. EMC requirements. General requirements.
LVS EN 61010-1: 2011
Safety requirements for electrical equipment for
measurement, control, and laboratory use. General
requirements.
LVS EN 61010-2-020: 2006
Particular requirements for laboratory centrifuges.

We declare that this product conforms to the requirements of the above Directives

____________________ ____________________
Signature Signature

Svetlana Bankovska Aleksandr Shevchik

Managing director Engineer of R&D

____________________ ____________________
Date Date

11
Biosan SIA
Ratsupites 7, build.2, Riga, LV-1067, Latvia
Phone: +371 67426137 Fax: +371 67428101
http://www.biosan.lv

Edition 3.04 – January 2018