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Trans Protime

The document outlines the procedure for measuring Prothrombin Time (PT), which assesses the extrinsic and common coagulation pathways by evaluating fibrin clot formation in citrated plasma. It details the preparation of reagents, incubation steps, and the significance of PT results, including reference ranges and potential causes for prolonged PT. Additionally, it highlights specimen collection errors that can affect test outcomes.

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Sofia Acsayan
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76 views1 page

Trans Protime

The document outlines the procedure for measuring Prothrombin Time (PT), which assesses the extrinsic and common coagulation pathways by evaluating fibrin clot formation in citrated plasma. It details the preparation of reagents, incubation steps, and the significance of PT results, including reference ranges and potential causes for prolonged PT. Additionally, it highlights specimen collection errors that can affect test outcomes.

Uploaded by

Sofia Acsayan
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

PROTHROMBIN TIME - Place the stopper

and mix.
- PRINCIPLE: - Let stand for no less
- This method is based o than 15 minutes.
measuring the formation - Reagent is stable at
time of the fibrin clot by 2-8C for 5 days
adding calcium 2. In a pre-heated tbe at 37C, place
thromboplastin to a citrated 100 uL sample. Incubate for 1
plasma minute in water bath at 37C. *the
- Detection of any alteration or actual first step*
deficiency of EXTRINSIC AND 3. Add the pre-heated PT reagent to
COMMON PATHWAY the sample and start the timer.
- Before the estimated coagulation
- Pre-operative screening test time, remove the tube from the
- Monitoring of therapy with oral water bath, gently slide the liquid
anticoagulant content from the bottom to the
middle of the tube and stop the
*uses semi-automated and full automated timer once with clot formation.
coagulation analyzers
*Blue top - sodium citrate anticoag of Reference range: 10-14 seconds
choice
Prolonged PT:
Procedure: - Liver disease
- Vitamin K deficiency
1. Pre-incubate a 200uL PT Reagent at - DIC
37C (no longer than 20 minutes).
- PT Reagent composition and Specimen collection errors:
preparation: - Short draw - 3mL
- Thromboplastin - Clot
- PL - Hemolysis
- Calcium - Lipemic
- Add 4mL purified or
distilled water if
powderized.
- Reconstitute the
contents of the vial
with the specified
volume of purified
water.

Acsayan, Sofia Andrei V.

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