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Microbiology Practical Guide

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0% found this document useful (0 votes)
91 views139 pages

Microbiology Practical Guide

Uploaded by

gspuneeth23
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

Nutrient Agar Nutrient Agar MacConkey agar

with Pseudomonas with staphylococcus


with LF NLF
aeruginosa and
colonies

Blood chocolate
agar agar

TCBS
agar

Citrate Test
Urease
Test Blue 7 Klebsiella
Pink t Klebsiella GreenC E coli
Yellow Ecoli
AST
Mueller
4948 b
Hinton
Agar

RCM

TS test
Red Yellow

acid is
produced
Anaerobic
jar
aka Candle jar

made of
Pt Ni
sterile straight
swab
wire
Inoculating used for
loop stroke stab
culture
BCG vaccine
PPD forMantoux test
Tuberculin
syringe

Universal
container

female is longer
than males
hasbuccalcapsule cylindrical
withteeth
copulatingbursa
with both
in posteriorend ends
female pointed
is longer
hascurved
end
cylindrical
pointed
at one
end Pinworm
Round Worm Hook Wolem enterobius
cg Ancylostoma vermicularis
Necater
Long segmented used
forpropagationembayonated
Doesoventrally ofviruses in lab
flattened egg

Routesofadministration
allantoic sac amniotic sac
Cam
yolk sac

3 parts head
neck strobila
Tapeworm
eg taenia solium

BHI broth peptone


water
Gram staining
Steps Primarystain
crystalviolet Gentianviolet for 1min
Rinsewithwater Violet Gram positive
Sep2 Mordant
Iodine 1min Pink
Rinsewithwater Gram negative
Paton
Rinse withwater Mechanism
CELL WALL THEORY
Stepscounterstain
Safranin 30s
Rinsewith water blot
Uses
i gram negative for identification
differentiation
of bacteria into gram positive
1 tostartempiricaltreatment
iii forfastidious organisms cgHaemophilus which taketime togrow in culture

iv forscreening qualityof specimen before processing it forculture

fy
Carbot fuchsin
Red acidfastbacilli
Blue puscells
5 minutes
Rinsewithwater
organismstructure sulfuricacid
H2 Intermittent
heatingbyflaming underneath needed
till fumesappear mordant Mtuberculosis 25
Step Decolonization M Leprae
Nocardia
25 sulphuricacid 2 4minutes Cryptosporidiumcyclospora s 1
Rinsewithwater
cytoisospora
steps counterstaining Bacterialspoke 0.25 0.5
Methylene blue for 30s
rinse withwater blot
Ascaris
Ancylostoma duodenale
Trichuais
trichiura
Entamoeba
histolytica
Giardia
lamblia
Ascaris
albuminous
Marylated
faked

io
Fertilized corticated
own

Fertilized
decorticated
Unfertilized

egg egg egg

Axoneme
inCentral
Karyosome

4 9
parabasal body JUL

i
Bacillary Dysentery
shigella dysenteriae
Bacillarydysentery shigellosis
shigelladysenteriae
etec Yersiniapestis
Phi

Tetrathionate broth
3 Enrichment broth selenite F broth Gramnegativebroth
Mildly selective MacConkey NLFcolonies
Agar
Highlyselective
5 Treatment
RE [Link]
Ciprofloxacin
DEC
[Link] [Link] Alternative Azithromycin ceftriaxone
complications Intestinal colon perforation rectalprolapse
toxicmega
sdysenteriaetype1 5days
Ekirisyndrome toxicencephalopathy
Bacteremia Hypoglycemia hyponatremia dehydration
Immunocompromised 7
10days
ORS
4 Virulence factors cellwallantigens Lipoteichoicacid Ccarb antigen Mprotein fimbria
Toxins hemolysinstreptolysin 0 5 SPE
Enzymes streptokinase streptodoanase Hyaluronidase
Cellulitis
Streptococcus pyogenes

E I
PRAGM
Direct microscopy gramnegative shortcommashaped fishinstream datingmotility hangingdrop
selectivemedia AlkalineBSA translucentoildropcolonics
TCBS largeyellowcolonies fermentsucrose
Monsua'sGITTA translucentcolonies
greyishblackcentre turbidhalo MacConkey translucentNLF whichbecomepink LF

stringtest positive Hemodigestion on blood greenish clearingaround onprolongedincubation


agar
0.51 Nadeoxy cholate loss
of maininoculum
turbidity becomesmucoid
3specimens freshlycollectedwaterystool rectalswab
Transportmedia VRmedium Alkalinesalttransport medium CaryBlair
Autoclaved
seawater

Cholera Vibriocholerae

Vcholerae V mimicus
Treatment i fluidreplacement ors i v V parahemolyticus

4 Antibiotics Azithromycin erythromycin V alginolyticus


minorrole
duration volume
offluidloss oforganism fromstool
clearance
Pseudomembrane toxin
bleedson removal adherent
ofepithelium
necrosis toxinformation mucosalulcers linedbyleatherygreyish
to mucosalbase white pseudomembrane
themembranecanbeeasily separated fibrin neutrophils RB

Diptheria Corynebacterium diphtheria

invivo
198
[Link]
lppttest
oftoxgeneper
detection

ofDILIA
detection

PEI boosterdose
ofdiphtheriavaccine penicillin G singledose erythromycin 7 10days
DT vaccination Pentavalent 6,1014weeks
1624months 5 years
10 16years
a T Early
stage aushours 20,000 40,000units
pharyngeal membranepresent 40,000 60000units
latestage 3days withbullneck 80,000 20,000units
1
e Antibiotics penicillin
of erythromycin
ifgivenearly 6has
prevent furtherrelease
oftoxin
Tx ofcarriers cutaneousdiphtheria
MDRsTyphi resistancetoChloramphenicol AmpicillinCotrimox
azole previouslyusedtotreatenteric
fever
Now Doe ceftriaxone azithromycin ciprofloxacin maybeaddedafterAST

Enteric fever Salmonella typhi

Rashes rose
spots on trunk chest
cerebellarataxia
muttering delirium
stepladderpattern
ofremittentfever
hepato
splenomegaly epistaxis

GI bleeding perforation
DCA NLF pale colonies with black center

xD red colonies withblackcenter


Wilson Blair
jetblack colonies withmetallic
Sheen due to Has production

Tubeagglutinationtest
2 folliculitis furuncle abscess impetigo hideadenitis suppurative necrotizingfasciitis psoasabscess
pyomyositis osteomyelitis mastitis breastabscess

Osteomyelitis Staphylococcus aureus

sepsis IE Toxicshock syndrome


Waterhouse Faiderichsen Syndrome SSSS
Paeruginosa Group B
Ecoli Klebsiella Proteus Tuberculosis streptococ

AST
kfactorsofSSI
Bm 35kgm
Diabetesmellitus

Age 6s
years
Anaemia
use
steroid
Longterm
Bloodtransfusion
Prematuresutureremoval
Inadequatesurgicalclosure
Highwoundtensionclosure
evention
ofSSI
Fullbodywash
Preparesurgicalsite incision
before
withporidoneiodine
clean
contaminatehands

Ifof
Use
[Link]

prophylacticantibiotic
Perioperative

site
seepsurgical closed fora minimum
of48hoursafterprocedure
Pseudomonas aeruginosa

surgicalsite infection Ssl streptococci


K pneumon
Candida Enterobacter Proteus Ecole
Staphylococci

CLABSI CAUT VAP 18 Swimmer'sear malignantotitisexterna


Shanghaifever
2 Early onset lateonset
S pneumoniae Paeruginosa
H influenzae Acinetobacter
MSSA E coli
VAP Klebsiella pneumoniae
Klebsiella

UTI Meningitis
septicemia
Pyogenic abscess
woundinfection s
Liverabscess
HAV Fecooral 4
HBV vertical
Percutaneous sexual
HCV Percutaneous sexual vertical
HDV Percutaneous sexual vertical
HEV Feworal
acute sphericalform

HBVinfection tubularfilamentous
complete
fogmatids

Recombinant Hep B vaccine


Foradults 0 1,6months
3doses 10 20augdos

NE Pentavalentat6,1
14weeks
Complications
1 secondarybacterialinfectionsotitismediabronchopneumonia recurrentfever
a Giantcellpneumonitis Hecht'spneumonia
a Acute laryngo bronchitis croup
trachea
Diarrhea
encephalitisESPE
subacutesclerosingpan
i Measlesinclusion
body encephalitis

[Link] iecautons
Vaccines liveattenuated vaccineSchwartz strain
reconstitution withdistilled water

NI MR at 9 12months 16 24months
0.5mL S C
CIP 10days [Link] iz
Measles
Eruptiverash in from
disappear
18
Post measlesweightloss
4 Inclusionbodies aggregatesofviralparticles orvirusinduced proteins
eitherin cytoplasm ornucleus
Intracytoplasmic Negri Rabies
Paschen small
pox
Peterson molluscumcontagiosum
a Intranuclear [Link] ffau iffdeeson

Cowdrytype B in poliovirus
Owl's
eyeinclusion inCMV
5 DogBite Management
I licks on intactskin localwound

IETaao
category
care

ategoayII Minorscratches abrasions [Link]


withoutbleeding 1M 1 1 1 1 Days 0 3,7 14 28
ategory III b
transdermal ites localwoundcare
1 rabiesvaccine
withoozing blood
of RIG
Negaibody usefultoconfirm
postmortem diagnosis

intracytoplasmic eosinophilic
inclusion bodies withbasophilic
inner granules
cerebellum
found in Purkinjecellsof
pyramidal cells ofhipocampus
stain Sellers
3 Veins cellline Tied
1 PurifiedchickembryocellPCC
a poofeed Venocell Pro
Iii HumandiploidcellHDC
humanembryoniclungfibroblast
line
3

Denguefever Influenza Measles


Malaria Chikungunya YellowFever Zika
NSL Ag detection
by ELISA ICT
MACelisa
Plague reduction microneutralization test
RDT fordengueIgMorNf
4 vaccineCainericYellowfever [Link]
IPh
Dengue

3 doses
j0.5mL
[Link]
each s c 6monthintervals
detection
of 3 UTRregionbyrealtin
reconstitution with
RTPCR
NS
SARS
SARS CoV 2 COVID 19virus

HCQ 400mgBD on Day1 400 onceweekly


mg for
next 7 days
COVID vaccine
mode
ofinfectioningestionofmaturegetsthrough badwater
contaminated

Cystsbypass
gastricjuice
[Link]
toreachsmallintestine

Maturecyst
Entamoeba
[Link]
4GG9 proteases

hydrolytic
enzymes
Immature atrophotoite
ye areadeased
histolytica

It
intestine

Encystations
Reachlarge multiply

[Link]
yfeintestnalmucos enteypgatal
invademistinal
[Link] intestine

amoebic formmaturequadrinucleatedcyst
Infective
liver

Iff fi i i
team
Diagnostic seasef
i
abscess

Host onehost man


I Luminalagents Jodo
quinol20days
Tissueagents Metronidazole
of 10days
Paromomycin

s 10days
of tinidazole 3days
Asymptomaticcarriage onlyluminalagents
Amoebicdysentery luminal tissueagents

Amoebic dysentery
Entamoebahistolytica

Intestinalamoebiasis
Fulminant colitis Amoebic appendicitis
Neurocysticercosis

Taeniasolium

intestinal taeniasis
Neuro
cysticercosis larvalstage
of7solium
Definitive host Man
intermediate

ofcontaminatedfoodwater of
rupture eggsinhuman
gut
fi
ingestion
[Link] 9fauae
auto
infection
of
release oncosphere
30 soy
penetration
ascyst larvae varioustissues circulation
deposited
ofintestine
Filariasis
Wachereria bancroft

DEC 6mgkg daily for 12 days


albendazole 400mg BD
for
21 days
ivermectin 0.4mg
kgsingledose
Tx Cutaneouscandidiasis oralcandidiasis
thrush Topicazoles
Esophageal vulvovaginal oral fluconazole
Disseminatedcandidiasis
B ofcapsofungin
liposomalamphotericin
Cglabrata Cauris liposomalamphotericinB
ofcapsofungin
oral Candidiasis
Candidaalbicans

Ctropicalis Cglabrata
C Kausei Cauris
Extremes
ofage infancy oldage C viswanathii
Pregnancy
Diabetesmellitus
steroidtherapy
febrileneutropenia
Post transplantation
HIVinfection iron deficiency
I Cryptococcosiswithoutcns involvement Fluconazole
with CNS involvement HIVinfection
induction phase 2weeks
maintenance
amphotericin B flucytosine
phase tillCD47
[Link] en9ngftiblucona2de

Cryptococcusneoformans
E C gatti

CSF blood skinscraping lumbarpuncture

Advanced HIV infection CDU Tcellcount 200 ML


Hematologic malignancies
Transplant recipients
Immunosuppressive or steroidtherapy
3 If oralteabinafine of itraconazole
skinlesion 1 2weeks
hairinfection 6weeks
onychomycosis 3 months
Deamatophytosis superficialmycosis

Taichophyton rubrum
Pulmonary aspergillosis

Aspergillus fumigatus

A fumigatus Aflavus A niger

Allergicbronchopulmonary aspergillosis ARPA


otitis externa Invasivesinusitis
mycotoxicosis cardiac aspergillosis endocarditis pericarditis
livercarcinoma Brain abscess meningitis
Keratitis endophthalmitis
1786renal
end stage
A

disease
irontherapy
steroidtherapy
neutropenia
Mucormycosis
Mucor racemose
Amphotericin B deoxycholate

Rhizopus arrhizus
Rhizomucoepusillus
Lichthermia corymbifera

Apophysomyceselegans

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