Lesson Plan 3
Emp Code: GUSBBS202433715
Emp Name: Dr. Amitabh Biswas
Course Code: Q1UA402T
Course Name: Biological-Analytical Techniques
Semester: IV
Academic Year: 2024-25
Title of your lesson: Non-Mechanical Methods of Cell Disruption
Course: Biological-Analytical Techniques
Aims:
To introduce students to the principles and applications of non-mechanical cell
disruption techniques.
To familiarize students with various non-mechanical methods used for cell lysis.
To enable students to understand the advantages and limitations of each technique.
Learning Outcomes: By the end of this lesson, you should be able to:
(1) Explain the basic principles behind non-mechanical cell disruption.
(2) Describe the different non-mechanical methods used for cell lysis.
(3) Compare and contrast the advantages and disadvantages of each technique.
Intended SOLO taxonomy Level: Relational/Multistructural
Table of Activities:
How you (the
teacher) will
What the students will Rationale for This
Activities facilitate or Duration
do Activity
scaffold student
learning
Briefly review key
To ensure students
concepts of cell
have a foundational
Recap of the previous structure and the
understanding
Recap session related to cell importance of 5 mins
before introducing
structure and function. accessing
disruption
intracellular
techniques.
components.
Opening Students will participate Facilitate the To engage students 10 mins
(Engaging in a discussion on why discussion by and introduce the
question to open it's necessary to break providing topic's relevance.
the lecture + cells non-mechanically. examples of
Opening Question:
"What are some
applications and
scenarios where we
Stating the LO) outlining the
might need to chemically
learning outcomes.
or enzymatically disrupt
cells?"
Students will be Use slides and
Activity 1: introduced to various visual aids to
To provide a
Introduction to non-mechanical cell explain each
comprehensive
Non- disruption techniques technique's 20 mins
overview of the
Mechanical (e.g., chemical lysis, principle,
available methods.
Methods enzymatic lysis, osmotic application, and
lysis). mechanism.
Students will work in
Provide a template
pairs to create a table To encourage
for the comparison
Activity 2: comparing the critical thinking and
table and guide the
Comparative advantages and peer learning by
students through 15 min
Analysis (Peer disadvantages of two comparing and
the process.
Learning) assigned non-mechanical contrasting
Facilitate a short
cell disruption techniques.
peer discussion.
techniques.
Present real-world
Students will discuss scenarios and
To help students
potential applications of encourage students
Activity 3: understand the
each non-mechanical cell to apply their
Application practical 15 mins
disruption technique in knowledge to
Scenarios applications of cell
different research or select the most
disruption.
industrial settings. appropriate
technique.
Students will summarize
the key learning points To reinforce
Facilitate a brief
and reflect on the learning and
Q&A session and
Conclusion importance of selecting address any 5 mins
summarize the
the appropriate non- remaining
main takeaways.
mechanical cell questions.
disruption technique.
Export to Sheets
References (Links and Textbooks):
1. [Link]
%20Cell%20disruption%[Link]
2. [Link]
hl=en&lr=&id=5QceAAAAQBAJ&oi=fnd&pg=PA49&dq=Non-
Mechanical+Methods+of+Cell+Disruption&ots=QLqtKVoZNA&sig=-
I2Zjq3V3qVVroLJFNAcDh2db9k&redir_esc=y#v=onepage&q=Non-Mechanical
%20Methods%20of%20Cell%20Disruption&f=false
3. Gomes, T. A., Zanette, C. M., & Spier, M. R. (2020). An overview of cell disruption
methods for intracellular biomolecules recovery. Preparative Biochemistry &
Biotechnology, 50(7), 635–654. [Link]
4. [Link]
Expectations:
Clearly articulate learning outcomes in your slides.
Include one core student activity that involves peer learning.
We'll be working on activities that require students to analyze, apply, and evaluate the
information. This means thinking critically and creatively. I'm looking for students to
demonstrate your understanding at a deeper level.
