Cambridge IGCSE™

BIOLOGY 0610/2
Paper 2 Multiple Choice (Extended) End-of-Semester exam December 2022
45 minutes
*6425434103*

You must answer on the multiple choice answer sheet.

You will need: Multiple choice answer sheet

Soft clean eraser
Soft pencil (type B or HB is recommended)

INSTRUCTIONS
• There are forty questions on this paper. Answer all questions.
• For each question there are four possible answers A, B, C and D. Choose the one you consider correct
and record your choice in soft pencil on the multiple choice answer sheet.
• Follow the instructions on the multiple choice answer sheet.
• Write in soft pencil.
• Write your name, centre number and candidate number on the multiple choice answer sheet in the
spaces provided unless this has been done for you.
• Do not use correction fluid.
• Do not write on any bar codes.
• You may use a calculator.

INFORMATION
• The total mark for this paper is 40.
• Each correct answer will score one mark. A mark will not be deducted for a wrong answer.
• Any rough working should be done on this question paper.
 1

1 The diagram shows the apparatus at the beginning of an investigation into temperature change
during the germination of seeds. The temperature at the start of the investigation was 25 °C in both
flasks.

After two days the temperature in flask 1 is 25 °C. The temperature in flask 2 is 28 °C.

vacuum
flask

dead and living seeds

disinfected
seeds

cotton wool

thermometer

flask 1 flask 2

Which characteristic of living organisms is shown in this experiment?

A excretion
B growth
C reproduction
D respiration
 2

2 The table shows the scientific names of four members of the cat family.

common name scientific name

leopard Panthera pardus
lion Panthera leo
ocelot Leopardus pardalis
tiger Panthera tigris

Which statement is correct?

A All four cats are members of the same species.

B The leopard and the ocelot are members of the same genus.
C The leopard, lion and tiger are members of the same genus.
D The leopard, lion and tiger are members of the same species.

3 Which structures are present in a bacterial cell?

cell wall nucleus

A ✓ ✓
B ✓ ✗
C ✗ ✓
D ✗ ✗
 3

4 The diagram shows an animal whose scientific name is Rattus rattus.

Which genus does it belong to?

A mammal
B rattus
C Rattus
D vertebrate

5 The diagram shows an arthropod.

Using the key, identify this arthropod.

1 body segments clearly visible .......................... go to 2

body segments not visible................................ go to 4
2 body with many segments................................ go to 3
body with three distinct parts............................ go to 4
3 antennae longer than thelegs.......................... A
antennae shorter than the legs.......................... B
4 three pairs of legs............................................... C
more than three pairs of legs............................. D
 4

6 The shaded area of the diagram represents structures found in both plant and animal cells.

plant animal
cell cell
structures structures

Which cell structure is from the shaded area?

A cell membrane
B cell wall
C chloroplast
D large vacuole

7 A cell has an actual length of 0.2 mm. A student wants to make a drawing of this cell so that it
appears two hundred times bigger.

How long should the student draw his cell?

A 4 mm B 0.04 mm C 40 mm D 40 cm
 5

8 The diagram shows a cell from the leaf of a plant.

Which part contains the highest amount of magnesium?

B
C
D

9 What helps to support plants?

A pressure inwards on the cell vacuoles

B pressure inwards on the chloroplasts
C pressure outwards on the cell walls
D pressure outwards on the nuclei

10 What would increase the rate of diffusion of oxygen into an animal cell?

A decreasing the concentration gradient between the inside of the cell and the outside
B decreasing the temperature of the cell and its surroundings
C increasing the distance that the oxygen molecules have to travel
D increasing the surface area of the cell membrane
 6

11 Three equally sized pieces of potato were put into different concentrations of sucrose solution.
One piece of potato was put into distilled water.

The concentrations of sucrose solution were 0.2 g dm-3, 0.4 g dm-3 and 0.6 g dm-3.

The graph shows the change in mass of the potato pieces over a period of 60 minutes.

Which piece of potato was put into distilled water?

2
A
change in mass of 1 B
the potato pieces / g
0 C
0 time / minutes 60
–1 D

12 The data shows the results of an investigation on osmosis using sticks of potato.

concentration of length of potato

length of potato
sugar solution stick after
stick at start / mm
/ mol per dm3 24 hours / mm

0.6 60 54

Which statements explain this change in length?

movement of water cause of movement

The sugar solution has a higher water

A into the potato cells
potential than the potato cells.
The sugar solution has a lower water
B into the potato cells
potential than the potato cells.
The sugar solution has a higher water
C out of the potato cells
potential than the potato cells.
The sugar solution has a lower water
D out of the potato cells
potential than the potato cells.
 7

13 A potato is cut into four pieces. The four pieces are weighed and then each one is placed in a
different sugar solution.

After four hours, the pieces are taken out of the solutions, dried and re-weighed. The masses of
the pieces at the start and after four hours are shown in the table below.

Which solution is the most dilute?

mass at start (g) mass after four hours (g)

A 24 22
B 25 34
C 28 23
D 32 35
 8

14 The diagram shows a protease molecule catalysing the break down of a protein molecule.

What are the parts labelled P, Q, R and S?

enzyme product substrate active site

A P Q R S
B R S P Q
C S P Q R
D S R Q P

15 Which diagram shows the structure of DNA?

A B C D

A C A T A C A G
A C A T A C A T

T G T A T G T G
C A C G C A C G
G T G C G T G C
A C A T A C C T

G T G C G T G C
 9

16 A student set up a test-tube containing starch, water and amylase.

How could the student test whether the amylase had digested all the starch?

A Add Biuret solution.

B Add dilute hydrochloric acid.
C Add iodine solution.
D Weigh the test-tubes and contents before and after theexperiment.

17 Water is a good solvent.

What does this mean?

A It dissolves well in many other substances.

B It flows easily through vessels.
C It is permeable to gases.
D Many substances dissolve well in it.

18 Which solutions are used for testing for protein, reducing sugar and starch?

test for reducing

test for protein test for starch
sugar

A Benedict’s iodine biuret

B biuret Benedict’s iodine

C biuret iodine Benedict’s

D iodine biuret Benedict’s

 10

19 The table shows whether starch was still present after four different experiments.

experiment result

starch + water at 20 °C ✓
starch + amylase at 20 °C ✗
starch + amylase at 30 °C ✗
starch + boiled amylase at 30 °C ✓

key

✓ = starch present

✗ = starch absent

What broke down the starch?

A amylase
B boiled amylase
C heat
D water

20 Which substance contains nitrogen?

A fat
B glucose
C protein
D starch
 11

21 Which statement about enzymes is correct?

A Enzymes become part of the product.

B Lowering the pH always slows down the reaction rate.
C Raising the temperature always increases the reaction rate.
D The specificity of an enzyme depends on the shape of its active site.

22 Why does excessive heat decrease enzyme activity?

A It changes the shape of the active site.

B It changes the shape of the substrate and product molecules.
C It increases the force of collisions between substrate and product molecules.
D It increases the kinetic energy of the substrate molecules.

23 The diagram shows an enzyme with its substrate and product molecules.

Which form an enzyme-substrate complex?

A P and Q B Q and R C R and S D S and P

24 What is the optimum pH for stomach enzymes?

A pH 2 B pH 7 C pH 9 D pH 12
 12

25 Four test-tubes were set up as shown in the table.

In which test-tube would starch be broken down the fastest?

2 cm3 starch
1 cm3 of 1 cm3 of boiled temperature
suspension
amylase added amylase added / °C
added

A ✓ ✓ ✗ 5

B ✓ ✓ ✗ 35

C ✓ ✗ ✓ 5

D ✓ ✗ ✓ 35

key

✓ = present

✗ = absent

26 Which statement about enzymes is correct?

A Enzymes are carbohydrates.

B Enzymes are catalysts.
C Enzymes are living organisms.
D Enzymes are the same shape as the substrate.
 13

27 The diagram represents an enzyme-controlled reaction.

What represents an enzyme?

A D

28 The diagrams show an experiment on enzyme activity.

The test-tubes contain hydrogen peroxide and the enzyme catalase.

In which test-tube is the enzyme inactive?

 14

29 The first diagram represents a chemical reaction.

reactants product

Which diagram, below, represents the enzyme for this reaction?

A B C D

30 During digestion, what is the function of an enzyme?

A to break down fats into droplets

B to break down food into small pieces
C to break down large molecules into small molecules
D to kill bacteria in food
 15

31 Curve X on the graph shows the effect of light intensity on the rate of photosynthesis.

rate of X
photosynthesis Y

light intensity

How have the conditions changed to produce curve Y?

A decreased concentration of carbon dioxide

B decreased light intensity
C increased concentration of carbon dioxide
D increased light intensity
 16

32 Two pieces of an aquatic plant were placed into two different test-tubes, P and Q.

Each test-tube contained hydrogencarbonate indicator and was sealed and kept at 20 °C.

Test-tube P was kept in the light and test-tube Q was kept in the dark.

The table shows the effect of carbon dioxide on the colour of the hydrogencarbonate indicator.

less carbon more carbon

dioxide dioxide

dark red orange

What would the colour of the indicator be after 12 hours?

P Q

A dark red orange

B dark red dark red
C orange dark red
D orange orange

33 Plants manufacture their own supplies of carbohydrate.

What are the raw materials and the waste product of this process?

raw materials waste product

A carbon dioxide and chlorophyll oxygen

B carbon dioxide and water oxygen
C oxygen and chlorophyll carbon dioxide
D oxygen and water carbon dioxide
 17

34 What is formed first in a leaf as a result of photosynthesis?

A chlorophyll
B glucose
C starch
D water

35 Which row shows the effects of deficiencies in nitrate and magnesium ions on plant growth?

effect of nitrate ion effect of magnesium

deficiency ion deficiency

A green leaves long roots

B long roots thin stems
C stunted growth yellow leaves
D thick stems large leaves

36 Four test-tubes are set up as shown in the diagram and left in full sunlight.
After several hours, which test-tube contains the most dissolved oxygen?
 18

37 Apparatus was set up as shown.

Sodium hydroxide removes carbon dioxide from the air. Limewater goes cloudy if carbon dioxide
is bubbled through it.

What happens to the limewater in flasks X and Y when the pump is switched on?
flask X flask Y

A goes cloudy goes cloudy

B goes cloudy stays clear

C stays clear goes cloudy

D stays clear stays clear

38 The diagram shows the apparatus used in an investigation to measure the rate of oxygen

production during photosynthesis.

The investigation was repeated several times and the average amount of gas collected was

calculated.

Which two factors must be kept constant during this investigation?

A the amount of water in the beaker and the height of the measuring cylinder

B the size of aquatic plant and the amount of gas in the measuring cylinder

C the size of aquatic plant and the duration of exposure to light

D the size of the beaker and the funnel

 19

39 The diagram shows a section through a leaf.

Which cell type absorbs the most carbon dioxide during the day?

40 Cobalt chloride paper is blue when dry but turns pink when wet. Some blue cobalt chloride paper
was fastened to the upper and lower surfaces of a leaf on a plant X and a leaf on plant Y.

The diagram shows the results of the experiment.

Through which leaf surface was water lost most quickly?

A plant X, upper surface

B plant X, lower surface

C plant Y, upper surface

D plant Y, lower surface