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Bead Beating Short Protocol Tables

The document outlines optimized lysis protocols using various mechanical lysis machines validated with the ZymoBIOMICS™ Microbial Community Standard. It details specific conditions for each machine, including recommended speeds, durations, and rest periods for effective bead beating. Additionally, it notes that certain devices, like the TissueLyser II and Retsch Mixer Mill MM 400, are not validated for microbiome research.

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71 views1 page

Bead Beating Short Protocol Tables

The document outlines optimized lysis protocols using various mechanical lysis machines validated with the ZymoBIOMICS™ Microbial Community Standard. It details specific conditions for each machine, including recommended speeds, durations, and rest periods for effective bead beating. Additionally, it notes that certain devices, like the TissueLyser II and Retsch Mixer Mill MM 400, are not validated for microbiome research.

Uploaded by

rosca
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

Optimized Lysis Protocols

The following conditions with different mechanical lysis machines were validated with
minimum bias using the ZymoBIOMICS™ Microbial Community Standard (D6300).

1 Vortex Genie with 2ml BashingBead™ 2 Bertin Precellys Evolution with 2 ml


Tubes BashingBead™ Tubes
Recommended for ease of use and Recommended for ease of use and ultra-
accessibility high speed.

Use Microtube Adaptor (Scientific 1. 1 minute on at 9,000 RPM


Industries, Inc. Cat. No. S5001-7)
2. 2 minutes rest
1. 40 minutes of continuous bead
beating (max of 18 tubes per 3. Repeat cycle 4 times for a total of 4
adaptor) minutes of bead beating

3 MP Fastprep-24™ (Classic & 5G) with 4 Omni Bead Ruptor Elite with 2 ml
2 ml BashingBead™ Tubes BashingBead™ Tubes

Maximum of 20 tubes. The weight of > 20 1. 1 minute on at 6 m/s


tubes may cause a system error.
2. 5 minutes rest
1. 1 minute on at 6.5 m/s
3. Repeat cycle 3 times for a total of 3
2. 5 minutes rest minutes of bead beating

3. Repeat cycle 5 times for a total of 5


minutes of bead beating

5 Biospec Mini-BeadBeater-16 with 2 ml 6 Biospec Mini-BeadBeater-96 with 2 ml


BashingBead™ Tubes BashingBead™ Tubes

1. 1 minute at maximum speed 1. 5 minutes on at Max RPM

2. 5 minutes rest 2. 5 minutes rest

3. Repeat cycle 5 times for a total of 5 3. Repeat cycle 4 times for a total of 20
minutes of bead beating minutes of bead beating

7 Biospec Mini-BeadBeater-96 with 96 well O TissueLyser II


lysis rack
No tested conditions yielded accurate
1. 5 minutes on at Max RPM profiles. This device is not validated by
Zymo Research for microbiome research.
2. 5 minutes rest

3. Repeat cycle 8 times for a total of 40


minutes of bead beating

O TissueLyser LT O Retsch Mixer Mill MM 400

No tested conditions yielded accurate No tested conditions yielded accurate


profiles. This device is not validated by profiles. This device is not validated by
Zymo Research for microbiome research. Zymo Research for microbiome research.

Ver. 1.0.3 Revised on 5/4/2022

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