Format (Practical Examination / OSPE)
MBBS Second Professional
GENERAL PATHOLOGY AND MICROBIOLOGY
S. No. COMMENTS MARKS
1. • OSPE
16 Stations (16 non-observed stations related to
64
practicals (each of 04 marks)
04 minutes at each station.
2. • Observed Practical
16
Microbiology
3. • STRUCTURED VIVA VOCE (related to curriculum) 50
25+25
(External + Internal)
4. • ANNUAL WORK BOOK 05
(External)
5. • CONTINUOUS INTERNAL ASSESSMENT 15
Total: 150
MBBS Second Professional Examination
GENERAL PATHOLOGY AND MICROBIOLOGY
TOS for OSPE
MARKS: 80 (04 marks per station)
TOTAL STATIONS=20
Time allowed= 01 hour &20 minutes (04 minutes per station)
TABLE OF SPECIFICATIONS
1) GENERAL PATHOLOGY (35% weightage, 07 stations : Non observed)
2) MICROBIOLOGY (65% weightage, 09 Non observed stations)
1) GENERAL PATHOLOGY: (07 unobserved stations)
Topic specification No. of Type of material given to
stations students
Inflammation Acute/Chronic/ 01 Microscopic slide/gross
Regeneration and Repair specimen/photograph/clinical
scenario as subject material
CELL INJURY & CELL DEATH 01 Microscopic slide/gross
(Necrosis, Fatty liver, Hyaline specimen/photograph/clinical
change) scenario as subject material
CELLULAR ADAPTATION: 01 Microscopic slide/gross
(Atrophy specimen/photograph/clinical
/Hypertrophy/Hyperplasia/Metaplasia) scenario as subject material
Pigmentation/ 01 Microscopic slide/gross
Intracellular accumulation/ specimen/photograph/clinical
Calcification. scenario as subject material
Neoplasia 02 Microscopic slide/gross
specimen/photograph/clinical
scenario as subject material
Hemodynamics-Thrombosis, 01 Microscopic slide/gross
Embolism, Infarction, Congestion specimen/photograph/clinical
scenario as subject material
2) MICROBIOLOGY: Total 09 stations
(09 Non observed stations)
Topic specification No. of stations Type of material given to students
BACTERIOLOGY: 02 Culture media, Blood agar, Mac-
(06 unobserved stations) Conkey agar, Lowenstein Jenson
medium, CLED, Nutrient agar.
01 Gram stained slide
01 Zeihl Neelson stained slide
01 Biochemical reactions/Motility
agar/urea agar/TSI
01 Photograph/Instrument/Report
interpretation related to bacteriology
Parasitology 01 Photograph/Specimen of a Helminth
(02 unobserved stations) /protozoa
01 Microscopic slide of ovum/egg/cyst
Serology / Immunology 01 Instrument/Micropipette/Elisa
(01 unobserved station) microtitre plate/Agglutination
reactions/Clinical scenarios/Report
interpretation.
MICROBIOLOGY (Observed Practical)*
Supervised by the Internal and External Examiners
Any one of the following.
Topic Specification Task to be performed Marks
Examination of Urine or Examine urinary
Stool sediment/detect protein in
urine/detect sugar in Procedure 4
urine/Microscopic Performance 8
examination of stool Result 4
Slide Coagulase Test Perform slide coagulase test
Catalase Test Perform Catalase Test
Smear Preparation for Prepare smear for staining Procedure 4
staining from the given clinical Smear Marking 8
specimen Staining 4
Result Interpretation 4
Procedure
*Any other procedure or task may be included.
Conduct of OSPE
• The Batches for Major viva voce and Practical / OSPE exam will be separate on any
particular day and will be 25 students strong each.
• All OSPE Questions will be sent by the Department of Examinations, UHS in sealed
confidential envelopes to each center clearly marked for each day of Examination and
shall be kept secure in our Regional Safety Lockers at respective centres.
• For any particular day of Examination the same OSPE questions will be sent to each
center to maintain standardization.
• The sealed confidential envelope containing the OSPE questions for that particular day
will be collected from the UHS regional safe locker by both the Internal and External
Examiners in the presence of the Principal or his nominee and the Regional Coordinator
up to Two hours before the commencement of Examination.
• Each packet of examination material will contain for that particular day the complete set
of sixteen non-observed OSPE questions with keys and instructions for the candidates
and the examiners.
• Instruction/ questions for the candidates will be included in the examination material and
should be placed on each station.
• The Practical Answer Books for non-observed stations will be sent separately to each
centre one for each candidate.
• The candidates are to carry the Practical Answer Books from station to station of the
non-observed stations and are to register their responses to each question at these
desks separately on the same Practical Answer Sheet in the designated areas.
• Before leaving the Assessment Hall the candidate should deposit the Answer Book
either at the “Marking Desk” or with the organizer as per decision of the convener.
• The candidates leaving the OSPE Hall will not mingle with candidates awaiting
assessment, who are to be kept under supervision in a separate holding bay.
• Each batch of the candidates while waiting for the OSPE in the waiting area should be
briefed about the OSPE process and the layout of the OSPE hall as well as the flow of
candidates through the hall. They are not to bring any mobile phones or any other
technology that could be used for communication within the premises of the
examination centre.
• Any student found having mobile phone or any other electronic medium should be
removed from the OSPE examination centre and an Unfair Means Case registered
against him/ her.
• All candidates will complete a mandatory “Feedback Proforma” and deposit the same
confidentially in the sealed collection boxes provided.
List of Practicals
1. Malarial Parasite.
2. Urine examination.
3. Stool examination (cyst of E. Histolytica, E. coli; Eggs of escaris, H. nana;
Entrobius vermicularis, Ancylostoma duodenale).
4. Culture media (Blood Agar, Mac Conkey Agar, Chocolate Agar, Nutrient Agar,
Sabourd Agar, CLED Agar, LJ Medium and Sensitivity Plate).
5. Blood culture.
6. Necrosis.
7. Gangrene.
8. Congestion and infarction.
9. Thrombosis.
10. Gram Staining.
11. ZN Staining.
12. Pigmentation.
13. Intracellular Accumulation.
14. Neoplasia; characteristics of malignancy.
15. Granuloma.
16. Anaerobic culture.
17. Acute inflammation (Acute appendicitis).
18. Chronic inflammation (Chronic cholecystitis).
19. Biochemical tests (Oxidase; Catalase; Coagulase; TSI; Urease; Citrate;
Indole; Sugars).
20. Benign epithelial tumours (Papilloma, Warts).
21. Malignant: epithelial tumours (squamous cell carcinoma; adenocarcinoma;
transitional cell carcinoma, basal cell carcinoma).
22. Benign connective tissue tumours (lipoma, leiomyoma).
23. Malignant connective tissue tumours (liposarcoma, leiomyosarcoma)
University of Health Sciences, Lahore
Second Professional MBBS
Annual / Supplementary Examination, 200____
OSPE Award List for General Pathology & Microbiology
College: _______________ Centre: _________________
Observed
Roll Non Observed Stations (Max Marks 64) Practical Grand Total
No. (Max Marks 16)
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 Total Total Figure Words
Examiners: Internal ________________________
External _______________________
University of Health Sciences, Lahore
Second Professional MBBS
Annual / Supplementary Examination, 200____
Structured Viva Voce Award List for General Pathology & Microbiology
College: ________________ Centre: _______________
Yearly Work Book Structured Viva Total
Roll Nos.
(Max Marks 5) (Max Marks 25) Figures Words
Internal Examiner: ______________________
University of Health Sciences, Lahore
Second Professional MBBS
Annual / Supplementary Examination, 200____
Structured Viva Voce Award List for General Pathology & Microbiology
College: ________________ Centre: _______________
Structured Viva Total
Roll Nos.
(Max Marks 25) Figures Words
External Examiner: ______________________
MBBS Second Professional Examination
GENERAL PATHOLOGY AND MICROBIOLOGY
Objectively Structured Performance Evaluation (OSPE)
Model Paper*
* This is a Model only and does not CONFORM to the Table
of Specification EXACTLY provided earlier in this
Document.
TOTAL STATIONS=16
TOTAL MARKS: 64 (04 marks per station)
Time allowed= 01 hour & 4 minutes (04 minutes per station)
MODEL PAPER
TABLE OF SPECIFICATIONS
1) GENERAL PATHOLOGY (35% weightage, 07 stations : all unobserved)
2) MICROBIOLOGY (65% weightage, 09 stations : all unobserved)
STATION#01 (UNOBSERVED STATION)
For Organizer:
SUBJECT MATERIAL: Microscopic slide showing features of acute appendicitis.
For Candidate:
Marks :04 Time Allowed:04 minutes.
TASK:
1) Examine the focused slide and identify it. (01)
2) Name the type of INFLAMMATION. (01)
3) Name the cell characteristic of this type of inflammation. (01)
4) Give TWO outcomes of this type of inflammation. (01)
For Examiner:
[Link] Key [Link]
1. Section of appendix showing acute inflammation (Acute appendicitis). 01
2. Acute inflammation. 01
3. Polymorphonuclear Neutrophil. 01
4. Complete resolution,Fibrosis,Chronic inflammation. 01
STATION#02 (UNOBSERVED STATION)
For organizer:
TOPIC SPECIFICATION: GENERAL PATHOLOGY/CELL INJURY AND CELL
DEATH/NECROSIS
SUBJECT MATERIAL: Microscopic slide (or photograph) showing a GRANULOMA with CASEOUS
NECROSIS in the centre.
For Candidate:
Marks :04 Time Allowed:04 minutes
TASK:
1) Examine the focused microscopic slide and identify the lesion. (01)
2) Name the type of necrosis seen in the left half. (01)
3) Name TWO other types of necrosis. (02)
For Examiner:
[Link] Key [Link]
1. Granuloma. 01
2. Caseous necrosis 01
3. Coagulative necrosis, liquefactive necrosis ,fat necrosis 02
STATION#03 (UNOBSERVED STATION)
For Organizer:
SPECIFICATION: GENERAL PATHOLOGY/CELLULAR ADAPTATIONS.
SUBJECT MATERIAL: Gross specimen or photograph of heart showing HYPERTROPHY.
For Candidate:
Marks :04 Time Allowed:04 minutes.
TASK:
Examine the specimen and answer the following questions.
1) Is the heart enlarged? Name the process of cellular adaptation that has occurred and define it.
(02)
2) Name TWO other types of cellular adaptation. (02)
[Link] Key [Link]
1. Yes. 02
HYPERTROPHY: An increase in the size of cells, resulting in an (0.5+01+1.5)
increase in the size of the organ.
2. Hyperplasia, Atrophy,Metaplasia. 02
STATION#04 (UNOBSERVED STATION)
For Organnizer:
TOPIC SPECIFICATION: GENERAL PATHOLOGY/CALCIFICATION.
SUBJECT MATERIAL: Microscopic slide or photograph of microscopic section of stomach along with
blood vessel showing DYSTROPHIC CALCIFICATION
For Candidate:
Marks :04 Time Allowed:04 minutes.
TASK:
1) Examine the focused slide and identify. (01)
2) Name TWO organs where metastatic calcification commonly occurs. (02)
3) What is the common cause of metastatic calcification? (01)
For Examiner:
[Link] Key [Link]
1. Calcification(DYSTROPHIC) 01
2. Kidney, blood vessels 02
3. Abnormal calcium metabolism 01
STATION#05 (UNOBSERVED STATION)
For Organizer:
TOPIC SPECIFICATION: GENERAL PATHOLOGY/NEOPLASIA
SUBJECT MATERIAL: Microscopic slide or photograph of LIPOMA along with clinical scenario.
For Candidate:
Marks :04 Time Allowed:04 minutes.
TASK:
Carefully read the clinical scenario and:
1) Examine the focused microscopic slide and identify. (01)
2) Is it a benign or malignant lesion? (01)
3) Name ONE other painless soft tissue swelling. (01)
4) Name the malignant form of this lesion. (01)
For Examiner:
[Link] Key [Link]
1. Lipoma 01
2. Benign 01
3. Fibroma. 01
4. Liposarcoma 01
STATION#06 (UNOBSERVED STATION)
For Organizer
TOPIC SPECIFICATION: MICROBIOLOGY/BACTERIOLOGY/CULTURE MEDIA
SUBJECT MATERIAL: Lowenstein Jenson (LJ) Medium Bottle
For candidate:
Marks :04 Time Allowed:04 minutes.
TASK:
1) IDENTIFY THE GIVEN MEDIUM? (01)
2) WHICH DYE IS ADDED TO THIS MEDIUM? (01)
3) WHAT IS THE USE OF THIS MEDIUM? (01)
4) GROWTH OF THE SPECIFIC ORGANISM WILL BE OBSERVED AFTER HOW MUCH
TIME ON THIS MEDIUM? (01)
For Examiner:
[Link] Key [Link]
1. Lowenstein Jenson Medium 01
2. Malachite Green 01
3. Medium is used for growth of Mycobacteria 01
4. 03-04 weeks 01
STATION#07 (UNOBSERVED STATION)
For Organizer:
TOPIC SPECIFICATION: MICROBIOLOGY/BACTERIOLOGY/CULTURE MEDIA
SUBJECT MATERIAL: Salmonella Shigella (SS) Agar plate inoculated with proteus species showing
Black colonies.
For candidate:
Marks: 04 Time Allowed: 04 minutes.
TASK:
1) IDENTIFY THE MEDIUM AND GROWTH. (01)
2) NAME TWO ORGANISMS THAT WILL GIVE SUCH BLACK COLONIES? (02)
3) NAME A SINGLE TEST THAT CAN HELP TO DIFFERENTIATE BETWEEN THESE
TWO ORGANISMS. (01)
For Examiner:
[Link] Key [Link]
1. Salmonella Shigella (SS) agar plate with H2S producing colonies. 01
2. Salmonella and Proteus. 02
3. UREASE TEST: Positive for proteus; Negative for Salmonella. 01
STATION#08 (UNOBSERVED STATION)
For Organizer:
TOPIC SPECIFICATION: MICROBIOLOGY/BACTERIOLOGY/GRAM STAINING
SUBJECT MATERIAL:
Gram stained smear showing gram –positive cocci in grape-like clusters.
For Candidate:
Marks: 04 Time Allowed: 04 minutes
TASK:
Examine the focused slide and answer the following questions:-
1) Is the organism Gram positive or negative? (01)
2) Give the morphology and arrangement of the bacteria seen in the smear. (01)
3) If on BLOOD AGAR culture the organism yields golden-yellow (Beta-hemolytic) colonies,
What is the likely organism? (01)
4)WHAT TEST WILL YOU PERFORM TO CONFIRM THE ISOLATE? (01)
For Examiner:
[Link] Key [Link]
1. Gram – positive. 01
2. Gram positive cocci in grape like clusters. 01
Staphylococcus – Aureus.
3. 01
Coagulase test (Staphylococcus is coagulase positive)
4. 01
STATION#09 (UNOBSERVED STATION)
For Organizer:
TOPIC SPECIFICATION: MICROBIOLOGY/BACTERIOLOGY/ZN STAINING
SUBJECT MATERIAL:
Sputum smear stained with Zeihl Neelson (ZN) stain showing Acid fast Bacilli (AFB).
For Candidate:
Marks: 04 Time Allowed: 04 minutes.
TASK:
1) Examine the slide and give microscopic findings. (1.5)
2) Name THREE organisms with such characters, (1.5)
3) What should be done to confirm the diagnosis in such patients? (01)
For Examiner:
[Link] Key [Link]
1. ZN stained slide showing acid fast bacilli (AFB) as bright 1.5
pink rods.
2. Mycobacterium Tuberculosis, Mycobacterium bovis, 1.5
Mycobacterium Kansai.
3. Culture on Lowenstein Jenson (LJ) Medium. 01
STATION#10 (UNOBSERVED STATION)
For Organizer:
Marks: 04 Time Allowed: 04 minutes.
TOPIC SPECIFICATION: MICROBIOLOGY/BACTERIOLOGY/BIOCHEMICAL REACTIONS.
SUBJECT MATERIAL:
TSI (Triple Sugar Iron) showing KA+- reactions.
For Candidate:
Marks: 04 Time Allowed: 04 minutes.
TASK:
1) Report the reactions observed in the provide TSI medium? (02)
2) What is the likely organism that would give these reactions? (01)
3) Is this organism motile or non-motile? (01)
For Examiner:
[Link] Key [Link]
1. Alkaline slant, Acidic butt, H2S positive, Gas negative. 02
2. Salmonella typhi. 01
3. Motile. 01
STATION#11 (UNOBSERVED STATION)
For Organizer:
Marks: 04 Time Allowed: 04 minutes.
TOPIC SPECIFICATION: MICROBIOLOGY/BACTERIOLOGY/ INSTRUMENT
SUBJECT MATERIAL: ANAEROBIC JAR
For Candidate:
Marks: 04 Time Allowed: 04 minutes.
TASK:
1) Identify the instrument. (01)
2) What is placed under the lid? (01)
3) Name the indicator used. (01)
4) Name TWO organisms that require anaerobic growing environment for growth.
(01)
For Examiner:
[Link] Key [Link]
1. Anaerobic jar. 01
2. Catalyst. 01
3. Methylene blue indicator. 01
4. Clostridia, Anaerobic cocci, Bacteroides. 01
STATION#12 (UNOBSERVED STATION)
For Organizer:
Marks: 04 Time Allowed: 04 minutes.
TOPIC SPECIFICATION: MICROBIOLOGY/PARASITOLOGY/HELMINTHS
SUBJECT MATERIAL: SPECIMEN (or photograph) OF ASCARIS LUMBRICOIDES.
For Candidate:
Marks: 04 Time Allowed: 04 minutes.
TASK:
1) Identify the organism. (01)
2) Give morphological characters of this organism.(01)
3) What illness does it cause in human beings? (01)
4) What is the route of transmission? (01)
For Examiner:
[Link] Key [Link]
1. Adult of Ascaries Lumbricoides. 01
2. Round worm with anterior posterior end .GIT is well 01
formed.
3. Ascariasis. 01
Oral route.
4. 01
STATION#13 (UNOBSERVED STATION)
Marks: 04 Time Allowed: 04 minutes.
TOPIC SPECIFICATION: MICROBIOLOGY/PARASITOLOGY/CYST/OVA/EGG OF PARASITES.
SUBJECT MATERIAL: MICROSCOPIC SLIDE (or photograph) OF EGG OF HOOKE
WORM.
For Candidate:
Marks: 04 Time Allowed: 04 minutes.
TASK:Carefully examine the given slide of stool and:
1) Give the microscopic finding and name the organism. (01)
2) Write TWO characteristic features of the egg seen. (02)
3) Give ONE complications that can occur in persons infected with this organism?
(01)
For examiner:
[Link] Key [Link]
1. Egg of Hooke worm. 01
2. Thin egg shell with segmented ovum. 02
3. Iron deficiency anemia 01
STATION#14 (UNOBSERVED STATION)
For Organizer:
Marks: 04 Time Allowed: 04 minutes.
TOPIC SPECIFICATION: MICROBIOLOGY/ MYCOLOGY
SUBJECT MATERIAL:
SEBOURAUDS MEDIUM.
For Candidate:
Marks: 04 Time Allowed: 04 minutes.
TASK:
1) Identify the medium. (01)
2) What is the use of this medium? (01)
3) Name FOUR fungi that can grow on this medium. (02)
For Examiner:
[Link] Key [Link]
1. Sebouraud’s medium. 01
2. Growth of fungi. 01
3. Candida albicans, Aspergillus species, Tricophyton, 02
Epidermatophyton.
STATION#15 (UNOBSERVED STATION)
Marks: 04 Time Allowed: 04 minutes.
TOPIC SPECIFICATION:
MICROBIOLOGY/ MYCOLOGY.
SUBJECT MATERIAL:
CLINICAL SCENARIO and slide of growth obtained after culture of urine of the subject
patient.
A 60 year old diabetic male is catheterized for two weeks. His urine has become turbid
and whitish. His urine was cultured and the given slide was prepared from the obtained
growth.
For Candidate:
Marks: 04 Time Allowed: 04 minutes.
TASK:
Carefully read the given clinical scenario:
A 60 year old diabetic male is catheterized for two weeks. His urine has become turbid
and whitish. His urine was cultured and the given slide was prepared from the obtained
growth.
Examine the slide and answer the following questions:
1) Give microscopic findings. (01)
2) What is the likely organism? (01)
3) Why infection with this organism is common in diabetes mellitus? (01)
4) Name TWO other parts of the body where infection with this organism can occur.
(01)
For Examiner:
[Link] Key [Link]
1. Budding yeast cells. 01
2. Candida. 01
3. Diabetics have lower immunity so they are more susceptible to 01
such infections.
4. Mouth, Vagina in females. 01
STATION#16 (UNOBSERVED STATION)
For Organizer:
Marks: 04 Time Allowed: 04 minutes.
TOPIC SPECIFICATION:
MICROBIOLOGY/ SEROLOGY/DEVICE
SUBJECT MATERIAL:
96 WELL ELISA MICROTITRE PLATE.
For Candidate:
Marks: 04 Time Allowed: 04 minutes.
TASK:
1) Identify the given devise used in a serological technique. (01)
2) Name the serological technique in which it is used. (01)
3) Give FOUR clinical applications of this technique. (02)
For Examiner:
[Link] Key [Link]
1. ELISA microtitre plate. 01
Enzyme Linked Immunosorbant Assay (ELISA)
2. To detect antigen/antibodies of hepatitis B virus, to detect 01
antibodies against hepatitis C virus, HIV, Cytomegalovirus
3. (CMV), Hepatitis A virus.(0.5 marks each) 02
