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CERTIFIED REFERENCE MATERIAL
6 components: 1000mg/l each of Diisodecyl phthalate (mixture of isomers) [CAS:26761-40-0] ; Phthalic acid, bis-iso-
nonyl ester (technical) [CAS:68515-48-0] ; Phthalic acid,bis-2-ethylhexylester [CAS:117-81-7] ; Phthalic acid,
benzylbutyl ester [CAS:85-68-7] ; Phthalic acid, bis-butyl ester [CAS:84-74-2] ; Phthalic acid, bis-1-octyl ester
[CAS:117-84-0] in n-Hexane
Lot N: XXXXXX Ref N: F263205 Certification Date:XXXXXX
Barcode: XXXXXXXX
Component Certified Value* CAS Chemical Formula
and uncertainty
[µg/ml]
Diisodecyl phthalate (mixture of isomers) 1002.3 ± 11.1 26761-40-0 C28H46O4
Phthalic acid, bis-iso-nonyl ester 1000.0 ± 22.2 68515-48-0 C26H42O4
(technical)
Phthalic acid,bis-2-ethylhexylester 1002.8 ± 9.8 117-81-7 C24H38O4
Phthalic acid, benzylbutyl ester 1004.2 ± 9.7 85-68-7 C19H20O4
Phthalic acid, bis-butyl ester 1005.8 ± 9.7 84-74-2 C16H22O4
Phthalic acid, bis-1-octyl ester 1000.6 ± 10.1 117-84-0 C24H38O4
* WQP 5.15.1/2 The certified value was obtained gravimetrically and confirmed experimentally by GC/MS or HPLC
Density 0.6906 g/cm3 at 20°C
Starting Material Purity, Batch
Diisodecyl phthalate (mixture of isomers) 99.9% (41466735)
Phthalic acid, bis-iso-nonyl ester (technical) 99.9% (41466384)
Phthalic acid,bis-2-ethylhexylester 99.9% (41492727)
Phthalic acid, benzylbutyl ester 99.6% (41443781)
Phthalic acid, bis-butyl ester 99.9% (41480755)
Phthalic acid, bis-1-octyl ester 99.7% (41461785)
Storage Conditions: Store in a refrigerator at temperatures between 2°C to 8°C
Expiry Date: XXXXXXXXXX
Concept of Certification and traceability statement:
This certified reference material is produced by gravimetric measurement and dissolving the individual substances in n-Hexane .
The reported expanded uncertainty of measurement is stated as the standard uncertainty of measurement multiplied by the coverage factor k = 2, which for a
normal distribution corresponds to a coverage probability of approximately 95%. The standard uncertainty of measurement has been determined in
accordance with EA 4/02 and incorporates the uncertainties of the raw-material purity, the mass and the volume.
The metrological traceability is defined as the “property of a measurement result whereby the result can be related to a reference through a documented
unbroken chain of calibrations, each contributing to the measurement uncertainty”.
The metrological traceability is ensured through gravimetric measurement and dissolving of certified reference material/s (traceable to SI) from
laboratories/producers, accredited according to ISO 17034.
The measurement results are traceable to SI. All analytical balances used for the preparation of the solution are calibrated yearly under an in-house
procedure with class E1 and class E2 analytical weights, traceable to SI (DKD), and are checked daily.
Class A laboratory glassware is used.
The results from temperature measurement are traceable to SI. The thermometers used for solution's calibration are calibrated from an ISO 17025 accredited
laboratory. The ambient conditions are controlled with a hygrometer calibrated from an ISO 17025 accredited laboratory.
Both, purity of the starting materials and solvent, were checked using appropriate analytical instrument.
Intended use: For Laboratory Use Only
This CRM is intended for:
Calibration of TLC, GC/FID, GC/TCD, GC/ECD, GC/MS, GC/MS/MS, LC/UV, LC/MS and LC/MS/MS
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Validation of analytical methods
Preparation of “working reference samples”
Detection limit and linearity studies
This statement is not intended to restrict the use for other purposes.
Instructions for the correct use of this certified reference material:
This CRM can be used directly or can be diluted in an appropriate solvent. Only a clean class A glassware should be used. Do not pipet from container.
Obtained concentration (in mg/l) after dilution is a result from the multiplication of certified value of CRM concentration and the CRM's volume used for
dilution and divided into the flask's volume used for dilution. For quantitative analysis, we recommend analyzing this mixture separately, without mixing it with
other solutions, to ensure accurate results for every compound.
Stability and storage:
This CRM is with a guaranteed stability until ±5% of the certified concentration for a period of 24 months. Stability is guaranteed of an unopened original
packaging stored, as written in the section: Storage Conditions. Even if the product is stable at normal laboratory conditions, in order to increase its stability,
we highly recommend it to be stored in a refrigerator.
The product should be used shortly after opening to avoid concentration changes due to evaporation. Warranty does not apply to a product stored after
opening.
Hazardous situation:
The normal laboratory safety precautions should be observed when working with this RM. Further details for the handling of this RM are available in a safety
data sheet.
Level of homogeneity
This solution was mixed according to an in-house procedure (MQP 5.13.1) and is guaranteed to be homogeneous.
To ensure sufficient homogeneity of the sample prior to use thoroughly mix by inversion or sonicate.
Names of certifying officers:
Laboratory: Margarita Dimitrova
Manager: Krassimira Taralova
This document QF 5.17.1/1 version 1 is designed and the certified value(s) and uncertainty(ies) are determined in accordance with ISO Guide 31, ISO Guide
35,and Eurachem / CITAC Guides
This certificate relates solely to the lot number given above.
All processes (including generating of this certificate) are completely controlled by the specialized Computer-Aided-Manufacturing (CAM) software.
This Certified Reference Material was produced under a quality management system that is:
- Registered to ISO 9001 Quality Management System (Lloyd's Register Quality Assurance Ltd Cert No 0039638)
- Accredited according to ISO/IEC 17025
- Accredited according to ISO 17034
Additional Information
Analytical Data:
GC Conditions:
Column PerkinElmer Elite 5-MS 30m, 0.25mm, 0.25µm Oven Temperature Hold
Flow rate 1.0 ml/min Initial 150°C 0.5
Injector 250 °C 5°C/min 220°C 2
Injection volume 1µl split (split ratio 10:1) 3°C/min 275°C 13
Carrier gas He, constant flow
MS Conditions:
Transfer line 250°C Ionization mode EI
MS Source 230°C Mode Scan
MS Quad 150°C
Gravimetric Data
Component Purity Source Weighed Final Bulk/ Concen- Chemist
% Lot No quantity, g quantity, Standard tration ID
kg.10-3 Solution mg/kg
lot No
Diisodecyl phthalate (mixture of isomers) 99.9 41466735 0.032 3.2142 91962546 9946.0 AS
91962546 1.0077 6.9063 XXXXXXXX 1451.23 DG
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Phthalic acid, bis-iso-nonyl ester (technical) 99.9 41466384 0.11179 3.2712 91981783 34140 AS
91981783 0.2929 6.9063 XXXXXXXX 1447.90 DG
Phthalic acid,bis-2-ethylhexylester 99.9 41492727 0.09296 3.2792 91981776 28320.0 AS
91981776 0.3541 6.9063 XXXXXXXX 1452.02 DG
Phthalic acid, benzylbutyl ester 99.6 41443781 0.30246 3.0898 91974570 97498 AS
91974570 0.103 6.9063 XXXXXXXX 1454.08 DG
Phthalic acid, bis-butyl ester 99.9 41480755 0.11185 3.6128 91968296 30928.4 AS
91968296 0.3252 6.9063 XXXXXXXX 1456.32 DG
Phthalic acid, bis-1-octyl ester 99.7 41461785 0.11401 2.1742 91980823 52280 AS
91980823 0.1914 6.9063 XXXXXXXX 1448.88 DG
Abundance TIC: 5C10.1K.HX.D\data.ms
6400000
6200000
6000000
5800000
5600000
Dibutyl phthalate, T
5400000
Bis(2-ethylhexyl) phthalate, T
5200000
5000000
Benzyl butyl phthalate, T
4800000
4600000
Di-n-octyl phthalate, T
4400000
4200000
4000000
3800000
3600000
3400000
3200000
3000000
2800000
2600000
2400000
2200000
2000000
1800000
1600000
1400000
Diisodecyl phthalate (mixture of isomers, T
Phthalic acid, bis-iso-nonyl ester (tech, T
1200000
1000000
800000
600000
400000
200000
0
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