NAME: Malasan, Armina Joy B. DATE: September 2, 2023 SECTION: 1BSN-07 RATING: PHINMA UNIVERSITY OF PANGASINAN COLLEGE OF ALLIED HEALTH SCIENCES DEPARTMENT OF NURSING ACTIVITY NO. 5 COLOR TEST FOR PROTEINS AND SPECIFIC AMINO ACIDS ation and Structure of Amino Acids Structure of Proteins (Peptide Bond) Name of test, purpose of test, composition of reagent, positive results obtained. II. PROCEDURE: A. GENERAL PROTEIN COLOR TESTS (20 points) (Perform the following tests on 1 ml. each of 1% solution of egg albumin, glycine and phenylalanine) 1, Biuret Test In separate test tubes containing 1 ml. of each of the above solutions, add 1 ml. of 10% NaOH solution and drops of 0.1% cupric sulfate solution. To a fourth test tube containing 1 ml. of distilled water which serves as a blank, add the same amount of NaOH and CuSO4. Describe any color change that occurs. Record your results in the table on the next page. 2. Ninhydrin Test In separate test tubes of the above solutions, add 1 ml. of ninhydrin solution. To a fourth test tube containing 1 ml. of dilute ammonium hydroxide, add the same amount of ninhydrin solution, Heat the tubes in a boiling water bath for 3 minutes and observe the color changes which occur in each tube. Record your results in the table on the next page. 3. Xanthoproteic Test In separate test tubes containing the above solutions, add an equal volume of cone. nitric acid (CAUTION!) To a fourth test tube containing 1 ml. of tryptophan, add the same amount of cone. nitric acid, Heat t for 2 minutes in a boiling water bath. Write your results in the table on the next page. Why does nitric acid stains a yellow colar? : © The wkin is corded by altre acid whieh retults in ctiaging blisters, Jt integrates with the keratin protein i the-vkin 1 produce xarthoprteic aid, vtainiag The Vkin yellow ae a reyub. 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B, COLOR TEST FOR SPECIFIE AMINO ACIDS (30 Points) n’s Test for Tyrosine In 3 separate test tube, place 2ml. each of 1% egg albumin, 1% gelatin and 0.02% salicylic acid solutic ions. Add 3 drops of Millon’s reagent and heat the tubes in boiling water bath for 2 minutes. Note the color produced. Record your results in the table bel low. 2, Hopkins-Cole Test for Tryptophan In 3 separate test tubes, place 2 ml. each of 1% egg albumin, 1% gelatin and e few granules of casein suspended n 2 mi. o water. Add 2 mi. of Hopkins-Cole reagent to each tube and mix thoroughly. Hold each test tube in an inclined position and carefully allow 3 ml. conc. sulfuric acid (CAUTION!) to slide down the side of each tube, Let stand and observe the color at the zone of the two fluids. Record your results in the table below. 3. Sakaguchi Test for Arginine Scanned with CamScannerTo 5 ml. of gelatin solution, add 1 ml. of 10% NaOH and 1 ml. of 0.02% alpha-naphthol solution. After 3 minutes, add 3 drops of sodium hypobromite solution. A strong red color develops but fades quickly. The color can be stabilized by adding urea to destroy the excess hypobromite, Write your result in the table below. Repeat using Arginine 4. Lead Acetate Test for Labile Sulfur To 2 ml. of 1% egg albumin in test, add 5 ml. of 5% NaOH solution and a few crystals of lead acetate. Heat in a boiling water bat for 5-10 minutes, with occasional mixing of the contents of the tube. Note the color change. Record your result in the table below. 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