PHINMA UNIVERSITY OF PANGASINAN COLLEGE OF ALLIED HEALTH SCIENCES DEPARTMENT OF NURSING BIOCHEMISTRY LABORATORY NAME: MALASAN, ARMINA JOY B. |UGUST 22, 2023 ACTIVITY NO, 2 pH and BUFFERS PH, hydronium ion concentration, buffers Henderson-Hasselbalch Equation: pH = pKa + log Use of pH paper (litmus paper) and pH meter Il- PROCEDURE: ‘A. DETERMINATION OF pH 1. Classify the following substances if tis acidic or basi. ‘Complete the table below: SAMPLE CLASSIFICATION 1. milk pH= 6.5 acidie 2. egg white pH=9.2 basic 3. vinegar pH=2.5 acidie 4. coke PH= 2.6 acidic 5. pepsi pH= 2.5 acidic 6. coffee pH= 4.8 basic 7. tea phe 435 acidic 8. calamansi juice pH= 24 acidie 9. chlorox pH= 11.4 basic 10. shampoo pH= 3.5 acidic TT, soap solution pH=9 basic 12. detergent solution basic 13. rubbing alcohol pH= 728 basic 14, hydrogen peroxide p= 62 acidie 15. beer pH= 4 acidic 16. baking soda soln. pH=9 basic Scanned with CamScannerIll- QUESTIONS: 1. Give the meaning of pH and buffer. A buffer is an element or combination of substances that, by nature of their existence in the solution, resist changes in pH caused by the addition of small amounts of acid or alkali. We use pH, a scale that utilizes logarithms, to measure how basic or acidic a liquid solution is. 2. Give the importance of pH. The pH of a solution of something or a product reflects its chemical makeup and surrounding conditions, making it an essential factor. It can regulate the biochemical reactions occurring inside living things, the activity of microbes, the transportation of vital nutrients, the behavior of enzymes and other chemical genera, and the ideal conditions under which different chemical species can thrive. 3. Why is it that the pH of body fluids like blood remains virtually constant? In order to prevent significant changes in the pH of our blood, our body contains multiple autonomous regulatory mechanisms, or "homeostatic systems,” that work together. Acid-base buffer systems are one of the body's strategies for controlling pH. The ability to stabilize pH in an aqueous solution is the function of buffers. When blood pH is excessively acidic, alkaline (base) buffers are released into the bloodstream, and vice versa, acidic buffers are activated when blood pH is too alkaline. 4, Calculate the [H®] and [OH] of a solution with pH 5.6 me be 7 Cow): ee Slot [5x CH} 2 10? (ord: yx aoc? Cat} = 2.561078 5. Calculate the pH of the buffer solution formed when 0.3M acetic acid (Ka = 1.8 x 10°*) is added to 0.2M sodium acetate, O.3M Acttic Acid + conjugate acid 2m Sodium Acotale + eojugale base 5 Ka? 1.8 x 407 par way 4 I9g (97/03) pka = -legCko) pka z -log64.8x 10°75) pH: 4.56, peas 4 My agi = pie pha 410g Cane bare) Scanned with CamScannerIV- Reference/s used: (APA format 7" edition) Helmenstine, A. M., PhD, (2022). Do You Know If Milk Is an Acid or a Base? ThoughtCo. https:/www.thoughtco.com/milk-an-acid-or-a-base-60736) Cotton, S. (n.d.). Coca-Cola. RSC Education. https://edu.rse.ore/soundbite/coca- cola/2021233.article ‘The unsweetened truth. (2015, July 14). Drinks Destroy Teeth. https:/drinksdestroyteeth.orp/the-unsweetened-truth Lane, T. (2021). Why Coffee Acidity Matters and Which Roasts are Most Acidic. Taylor Lane. https://www.taylorlane.com/blogs/read/is-coffec-an-acid-or-a-base Malone, M. (2020, April 6). Tea Brew Guide: How water quality affects flavors & clarity. Perfect Daily Grind. https:/perfectdailyerind.com/2017/1 /tea-brew-guide-how-water-quality. affects-flavors-clarity/ C, J. (n.d.). Understanding the characteristics of detergent products from Ph. wwwlinkedin.com. https:/www linkedin.com/pulse/understanding-characteristics-detergent- products-from-joey-chan lucts-from-j ‘Whaat is the pH of chlorine dioxide? - Answers. (2023, August 10). Answers. https:/wwwanswers.com/chemis is the orox bl Helmenstine, A. (2023, May 2). The pH scale of common chemicals. Science Notes and Projects. https://sciencenotes.org/the-ph-scale-of-common-chemicals/ Scanned with CamScanner