D. Y.

PATIL, MEDICAL COLLEGE, KOLHAPUR

1 YEAR MBBS QUESTION BANK

ST

BIOCHEMISTRY
MULTIPLE SCHOICE QUESTION:
1) The earliest serum enzyme to increase after myocardial infarction is

a) Creatine kinase b) LDH

c) GOT d) GPT
2) Point out the essential amino acid
a) Lysine b) Tyrosine
c) Glycine d) Proline
3) The site at which ES complex normally forms is
a) Allosteric site b) Inhibitor site
c) Active site d) None of the above
4) Coenzyme A is the coenzyme form of
a) Vitamin A b) Pantothenic acid
c) Niacin d) Biotin
5) The specific dynamic action (SDA) for proteins is
a) 10%
b) 20%
c) 30%
d) None of the above
6) Tryptophan is the precursor for
a) Melatonin b) Tryptamine
c) Serotonin d) All of the above
7) Which of the following pathway occurs partly in cytosol and partly in mitochondria?
a) Urea cycle b) Glycolysis
c) TCA cycle d) Oxidative phosphorylation
8) Point mutation is
a) Change in single base
b) Deletion of single nucleotide
c) Insertion of single nucleotide
d) Deletion or insertion of more than one nucleotide
9) Amino acid required for heme synthesis is
a) Lysine b) Glycine
c) Valine d) Alanine
10) Codons are present on
a) DNA b) m RNA
c) r RNA d) t RNA
11) Point out the auto-oxidizable cytochrome
a) Cyt. b b) Cyt. a 3
c) Cyt. c d) Cyt. c 1
12) Following are transport proteins, EXCEPT
a) Albumin b) Hemoglobin
c) Myosin d) Beta lipoprotein
13) Which of the following is intracellular enzyme?
a) Pancreatic lipase b) Amylase
c) Pepsin d) Hexokinase
14) Urea synthesis requires following, EXCEPT
a) NAD+ b) Ammonia
c) Carbon dioxide d) Aspartate
15) Methyl malonic acide is excreted in urine in the deficiency of
a) Folic acid
b) Thiamine
c) Cyanocobalamin
d) Niacin
16) The base NOT found in DNA is
a) Adenine b) Cytosine
c) Uracil d) Guanine
17) Positive nitrogen balance is observed in all, EXCEPT
a) Convalescence b) Fever
c) Pregnancy d) Growth
18) Bence Jones proteins excretion in urine is diagnostic of
a) Tuberculosis
b) Multiple myeloma
c) Diabetes mellitus
d) Diabetes insipidus
19) Normal serum alkaline phosphatase activity is
a) 3 – 13 mg/dl b) 3–13 IU/L
c) 3–13 KA units d) 3–13 gm%
20) An increase in serum unconjugated bilirubin occurs in
a) Hemolytic jaundice b) Obstructive jaundice
c) Defect in intestinal absorption d) Viral hepatitis
1) The enzyme present in liver but absent in muscles is
a) Phosphorylase
b) Glucose -6- phosphatase
c) Phosphoglucomutase
d) Debranching enzyme
2) The metabolically active form of calcium is
a) Protein bound b) Ionized
c) Both of the above d) None of the above
3) The best parameter to assess renal function is
a) Blood urea b) Urinary urea
c) Serum creatinine d) Serum uric acid
4) Bile pigments are NOT excreted in urine in
a) Obstructive jaundice b) Hepatic jaundice
c) Hemolytic jaundice d) All of the above
5) Transport of fatty acids into mitochondria requires
a) Keratin b) Carotene
c) Carnitine d) Carnosine
6) L – epimer of glucose is
a) Mannose b) Fructose
c) Galactose d) Idose
7) The acid - base disorder found in uncontrolled diabetes mellitus is
a) Respiratory acidosis b) Respiratory alkalosis
c) Metabolic acidosis d) Metabolic alkalosis
8) In the patient with renal failure, blood levels of all of the following are elevated, EXCEPT
a) Albumin
b) Urea
c) Creatinine
d) Uric acid
9) CA - 125 is used as a tumour marker for the cancer of
a) Bone b) Prostate
c) Ovary d) Lung
10) Net gain of ATP in conversion of glucose to lactate is
a) 2 b) 4
c) 6 d) 8
11) All of the following are amphipathic lipids, EXCEPT
a) Triacylglycerol b) Cholesterol
c) Phospholipids d) Fatty acids
12) Following is the transport form of iron
a) Haemoglobin b) Cytochrome
c) Transferrin d) Ferritin
13) Ingestion of sodium benzoate in man results in an increased urinary excretion of
a) Phenyl pyruvic acid b) Oxalic acid
c) Hippuirc acid d) Glyoxalic acid
14) Liver can not use ketone bodies due to absence of enzyme
a) Thiokinase b) Thiolase
c) Thiophorase d) Thionase
15) Urea level in blood may be elevated in all of the following conditions, EXCEPT
a) Dehydration
b) Renal failure
c) Hepatic failure
d) Hypertension
16) Absence of urobilinogen in urine occurs in
a) Obstructive jaundice b) Haemolytic jaundice
c) Hepatic Jaundice d) All of the above
17) The mineral having synergistic action with vitamin E is
a) Chromium b) Cobalt
c) Selenium d) Molybdenum
18) Which of the following hormones affects the fluid & electrolyte balance?
a) Epinephrine
b) Glucagon
c) Thyroxine
d) Aldosterone
19) The major intracellular cation is
a) Sodium b) Potassium
c) Calcium d) Magnesium
20) Biotransformation by conjugation requires
a) Glutathione b) Inulin
c) Tyrosine d) Mannitol
Biochemistry SAQ
1) What are Isoenzymes? Name the Isoenzyme forms of LDH and CK. How they are
useful in diagnosis of disease.
2) Give an account of purine salvage pathway indicating genetic disease associated with
it.
3) Explain Inhibitors and Uncouples of Oxidative phosphorylation giving examples.
4) Write application of Recombinant DNA technology in medicine.
5) Enumerate its merits.
6) Explain biochemical basis of Scurvy and Rickets.
7) Give reactions of biologically important products synthesized from glycine.
8) Explain with examples Primary and secondary structure of protein.
9) Explain Catabolism of Heme and add a note on Jaundice.
10) Transamination reactions.
11) Describe the steps of Purine Nucleotide degradation.
12) Define Respiratory Quotient (RQ). Write the RQ of carbohydrates, lipids and
proteins.
13) What are acute phase proteins? Give three examples with their functions.
14) Define Mutation. Name any three types of Mutations with examples.
15) What are essential amino acids? Name them.
16) Define uncouples. Give any three examples of physiological uncouplers.
17) Write the sources, recommended daily allowance and biochemical functions of
Vitamin A.
18) What is Polymerase Chain Reaction (PCR)? Describe the procedure and two
applications for PCR.
19) What are biologically important peptides? Give three examples and their biological
roles.
20) Define isoenzymes. Enumerate the isoenzyme forms and one clinical importance of
each. a) Lactate dehydrogenase b) Creatinine kinase.
21) Sickle cell anemia
22) Role of dietary fibre in diet
23) Salvage pathway for purines
24) Restriction endonuclease.
25) Urea cycle with inborn errors.
26) Applications of recombinant DNA technology.
27) tRNA
28) Folate trap
29) Purine salvage pathway
30) Enumerate the factors which influences enzyme activity
31) Thalassemia
32) Diagnostic and therapeutic uses of enzymes
33) Types and functions of immunoglobulins.
34) A 5-month old female infant was hospitalized. A diagnosis of classic phenylketonuria
(PKU) was made. Questions:1) Name defective enzyme in classic PKU 2) Name
other types of PKU with their defective enzymes 3) What are the characteristics of
PKU.
35) Explain the regulation of glycogen metabolism.
36) Give an account on fatty liver and lipotropic factors.
37) Give diagnostic applications of radioisotopes.
38) What are the factors affecting calcium absorption?
39) Explain kidney function tests based on glomerular function.
40) Write the functions of phospholipids.
41) What are the biologically important sugar derivatives of monosaccharide? Explain.
42) Explain the role of various blood buffers in the maintenance of blood pH.
43) Detoxication by conjugation.
44) Define lipoproteins. Explain the mechanism of reverse cholesterol transport by HDL.
45) b) Describe the reactions catalyzed by PDH complex. Write the significance of this
complex
46) Name Bile salts. Explain their formation and role in lipid digestion.
47) Write any four functions of copper and four disorders of copper metabolism.
48) What is meant by detoxification?
49) Give an account of any two detoxification processes.
50) Name any four Glycogen storage diseases with their enzyme defect.
51) Explain the Amphibolic nature of TCA cycle with suitable examples.
52) Describe in brief the Biochemical changes in starvation.
53) What is Vandenberg reaction? Write the principle and its application.
54) What are epimers? Give two examples of epimers of D-Glucose.
55) Clinical significance of plasma cholesterol level.
56) Functions of Iron.
57) Kidney function tests.
58) Chromatography.
59) Fatty liver.
60) Sources of iodine.
61) Functions essential fatty acids.
62) What is rancidity?
63) Galactosaemia.
64) Lactose intolerance.
65) Functions of manganese.
66) Explain diagnostic uses of radioisotopes.
67) What is PDH COMPLEX 7
68) What are tumor markers? d' Explain ketolysis.
69) Hyper lipoproteinemias.
70) Classify polysaccharides, Give two examples of homopolysaccharides.
71) Write functions of lipoproteins.
72) Name any three antioxidant vitamins.
73) Name any three liver function tests.
74) Describe digestion and absorption of carbohydrates.
75) Write functions of phospholipids.
76) What is cyclic AMP? What is its metabolic importance?
77) A chain smoker had sweating and chest pain. He was diagnosed as a case of
Myocardial Infarction (MI) a) State the relation between smoking and MI. b) Give the
risk factors of MI. c) State the prevention measures.
78) Give four examples of isotopes and its applications in medicine.
79) What are reactive oxygen species? Name three disease states associated with excess
production of reactive oxygen species.
80) Write the biological reference values of sodium and potassium. How is potassium
regulated in the biological system?