Clearing is otherwise called as: Bisphenol A, glycerol, and cyclohexene dioxide are all examples of:

a. Dealcoholization a. Paraffin wax

b. Dehydration b. Clearing agent
c. Blocking c. Plastic impregnating media
d. Infiltration d. Fixatives
The correct answer is: Dealcoholization The correct answer is: Plastic impregnating media

Triethyl phosphate is used in the histopathology laboratory as: The most commonly used clearing agent is:
a. Dehydrating agent a. Aniline oil
b. None of these choices. b. Toluene
c. Fixative c. Xylene
d. Decalcifying agent d. Chloroform
The correct answer is: Dehydrating agent The correct answer is: Xylene

Which of the following is used for deparaffinization? All of these are bluing agents, except:
a. 70% alcohol a. Lithium carbonate
b. Xylene b. Ammonia water
c. Acetone c. Acid alcohol
d. 95% alcohol d. None of these
The correct answer is: Xylene The correct answer is: Acid alcohol

EA 50 is a polychrome stain composed of three stains which include all of the Hydrogen peroxide and potassium permanganate convert the Hematoxylin
following, except: into:
a. Hematoxylin a. Mercuric oxide
b. Eosin Y b. Stable hematein
c. Light Green SF c. A weak base
d. Bismarck Brown d. A weak acid
The correct answer is: Hematoxylin The correct answer is: Stable hematein

Select from the list below the recommended fixative for embryos and Select the true statement/s below.
pituitary biopsies. a. Glutaraldehyde can be used as a fixative for enzyme
a. B-5 fixative histochemistry.
b. Methanol b. Acetone can be used as a fixative for blood containing organs.
c. Brasil’s Alcoholic Picroformol c. Both of these statements.
d. Bouin’s Solution d. None of these statements.
The correct answer is: Bouin’s Solution The correct answer is: Glutaraldehyde can be used as a fixative for enzyme
histochemistry.
Carazzi hematoxylin uses this kind of mordant:
a. Alum Benzene is used in the histopathology laboratory as:
b. Tungsten a. Decalcifying agent
c. Iron b. Dehydrating agent
d. Lead c. Clearing agent
The correct answer is: Alum d. Fixative
The correct answer is: Clearing agent
Which of the following is considered as the best fixative for tissues containing
iron pigments? The best general decalcifying agent is the:
a. Formol-calcium a. Trichloroacetic acid
b. 5% formic acid b. Hydrochloric acid
c. Glutaraldehyde c. 5% formic acid
d. 10% buffered neutral formalin d. Nitric acid
The correct answer is: 10% buffered neutral formalin The correct answer is: 5% formic acid

The fixative used for blood smears is: Which of the following is the routine fixative of choice for tissue
a. Methanol photography?
b. Boric acid a. Lead fixative
c. Formalin b. Acetone
d. Ethanol c. Osmium tetroxide
The correct answer is: Methanol d. Mercuric chloride
The correct answer is: Mercuric chloride
What should be the temperature of the water bath that is used to float tissue
cut from the microtome? All of the following fixatives are considered nuclear fixatives, except:
a. 30 C a. Orth’s fluid
b. 37 C b. Flemming’s solution
c. 45-50 C c. Heidenhain’s SuSa
d. 50-56 C d. Newcomer’s fluid
The correct answer is: 45-50 C The correct answer is: Orth’s fluid
The other name for Zenker-Formol is: Select the true statement/s below.
a. Ohlmacher’s fluid a. Both of these statements.
b. Helly’s solution b. None of these statements.
c. Schaudinn’s fluid c. Acrolein is classified as an aldehyde fixative.
d. Regaud’s fluid d. Schaudinn’s fluid is classified as a mercuric chloride fixative.
The correct answer is: Helly’s solution The correct answer is: Both of these statements.

Sectioning is otherwise called as: In routine tissue processing, the agent that follows the series of alcohols used
a. Blocking for dehydration is:
b. Impregnation a. Xylene
c. Casting b. Glutaraldehyde
d. Microtomy c. Saline
The correct answer is: Microtomy d. EDTA
The correct answer is: Xylene
Which of the following is used for cutting sections for electron microscopy?
a. Vibrotome The glacial acetic acid solidifies at:
b. Rocking microtome a. 4C
c. Ultrathin microtome b. 56 C
d. Freezing microtome c. 35 C
The correct answer is: Ultrathin microtome d. 17 C
The correct answer is: 17 C
Which of the following can be used as a decalcifying agent?
a. Trichloroacetic acid Which of the choices below is the correct REHYDRATION SEQUENCE of tissue
b. All of these choices sections before hematoxylin staining?
c. Formic acid a. Water…70% ethanol...80% ethanol...90% ethanol...95%
d. Picric acid ethanol...100% ethanol
The correct answer is: All of these choices b. Water…100% ethanol...95% ethanol...90% ethanol...80%
ethanol...water
The Calcium Oxalate Test is a way to measure: c. 100% ethanol…95% ethanol…80% ethanol…70% ethanol…water
a. The extent of dehydration d. Acetone…70% ethanol…80% ethanol…90% ethanol…95% ethanol
b. The extent of decalcification The correct answer is: 100% ethanol…95% ethanol…80% ethanol…70%
c. The acidity of a decalcifying agent ethanol…water
d. The extent of fixation
The correct answer is: The extent of decalcification It is probably the oldest of all stains, originally used for the microscopic study
of starch granules:
This is considered as the best general decalcifying agent: a. Methylene blue
a. 5% formic acid b. Orcein
b. EDTA c. Malachite green
c. 10% BNF d. Iodine
d. Perenyi’s fluid The correct answer is: Iodine
The correct answer is: 5% formic acid
Select the true statement/s below.
The usual length of the plane wedge knife is: a. Both of these statements.
a. 100 mm. b. Regaud’s fluid is classified as a mercuric chloride fixative.
b. 75 mm. c. Orth’s fluid is classified as an aldehyde fixative.
c. 50 mm. d. None of these statements.
d. 120 mm. The correct answer is: None of these statements.
The correct answer is: 100 mm.
The purpose of thymol crystals in Mayer’s Egg Albumin is to:
Which of the following terms is synonymous to embedding? a. Prevent drying
a. Blocking b. Prevent bacterial overgrowth
b. Dealcholization c. Stabilize the preparation
c. Orientation d. Prevent fungal overgrowth
d. Infiltration The correct answer is: Prevent fungal overgrowth
The correct answer is: Blocking
Dioxane is used in the histopathology laboratory as:
Select the true statement/s below. a. None of these choices.
a. None of these statements. b. Both of these choices.
b. Acetone can be used as a fixative for blood containing organs. c. Dehydrating agent
c. Both of these statements. d. Clearing agent
d. Glutaraldehyde can be used as a fixative for enzyme The correct answer is: Both of these choices.
histochemistry.
The correct answer is: Glutaraldehyde can be used as a fixative for enzyme All of the following fixatives are considered nuclear fixatives, except:
histochemistry. a. Bouin’s solution
b. Newcomer’s fluid
c. Carnoy’s fluid
d. Helly’s fluid
The correct answer is: Helly’s fluid
Which of the following hematoxylins is used for the study of 3-APES is an example of a/an:
spermatogenesis? a. Adhesive agent
a. Weigert’s hematoxylin b. Embedding medium
b. Copper hematoxylin c. Bluing agent
c. Cole’s hematoxylin d. Stain
d. Mallory’s PTAH The correct answer is: Adhesive agent
The correct answer is: Copper hematoxylin
Poly-L-Lysine is an example of a/an:
Which of the following may be used for the fixation of post-mortem tissues? a. Stain
a. 10% Formol-Saline b. Adhesive agent
b. None of the choices c. Bluing agent
c. Formol-Sublimate d. Embedding medium
d. Both of these choices The correct answer is: Adhesive agent
The correct answer is: Both of these choices
Which of the following may be used as a clearing agent?
In order to have an effective knife, which one of the following hones is a. 4% phenol
recommended because it gives the best honing result? b. All of these choices.
a. Paddle strop c. Methyl salicylate
b. Arkansas d. Anhydrous copper sulfate
c. Belgium yellow The correct answer is: Methyl salicylate
d. Fine Carborundum
The correct answer is: Belgium yellow The usual length of the biconcave knife is:
a. 25 mm.
Ester wax has all of the following characteristics, except: b. 50 mm.
a. Its melting point is 46-48 C. c. 100 mm.
b. None of these d. 120 mm.
c. It is soluble in the dehydrating fluid. The correct answer is: 120 mm.
d. It is soluble in water.
The correct answer is: It is soluble in water. This is the property of tissues to be stained with fat or oil-soluble dyes,
regardless of the type of dye used, due to their essential lipid nature:
Which of the following fixatives may also be used in dehydration? a. Hypochromia
a. Acetone b. Metachromasia
b. Both of the choices c. Sudanophilia
c. None of the choices d. None of these
d. Ethyl alcohol The correct answer is: Sudanophilia
The correct answer is: Both of the choices

The ultrathin microtome is chiefly utilized for cutting tissue (very thin
sections) at _______.
a. 0.5 micra
b. 4 to 6 micra
c. 10 to 15 micra
d. 2.0 micra
The correct answer is: 0.5 micra

Tetrahydrofuran is used in the histopathology laboratory as:

a. Dehydrating agent
b. None of these choices.
c. Clearing agent
d. Both of these choices.
The correct answer is: Both of these choices.

EPON, Spurr, and Araldite are examples of what infiltrating medium?

a. Plastic
b. Gelatin
c. Paraffin
d. Celloidin
The correct answer is: Plastic

Removal of formaldehyde or mercury pigments from tissue sections is

performed before:
a. Fixation
b. Sectioning
c. Dehydration
d. Staining
The correct answer is: Staining