ﻣﺨﺘﺒﺮ
أﺑﻮ ﻣـــــﻮدة
اﻟﻄﺒﻲ اﻟﺤﺪﻳﺚ
اﻟﻄﺒﻲ اﻟﺤﺪﻳﺚ
SOPs
ﻛﻴﻔﻴﺔ ﺗﺤﻀﻴﺮ ﻋﻴﻨﺔ اﻟﺒﻮل اﻟﻌﻤﻮﻣﻲ
وﺿﻊ اﻟﺸﺮﻳﻂ ) (Stripﻟﻘﻴﺎس اﻟﺴﻜﺮ و اﻟﺒﺮوﺗﻴﻦ و اﻻﺳﺘﻮن
ﻋﻤﻞ اﻟﺘﺮﻗﻴﻢ ﻓﻲ اﻻﻧﺒﻮب )(Tube
اﺿﺎﻓﺔ 10ﻣﻞ ﻣﻦ اﻟﺒﻮل ﻓﻲ اﻻﻧﺒﻮب و ﺗﺪوﻳﺮه ﺑﻮاﺳﻄﺔ
ﺟﻬﺎز اﻟﻄﺮد اﻟﻤﺮﻛﺰي ﺑﺴﺮﻋﺔ 3000ﻟﻔﺔ ﻟﻤﺪة 3دﻗﺎﺋﻖ
اﻟﺘﺨﻠﺺ ﻣﻦ اﻟﺮاﺷﺢ و وﺿﻊ اﻟﺮاﺳﺐ ﻓﻲ ﺷﺮﻳﺤﺔ زﺟﺎﺟﻴﺔ و وﺿﻊ اﻟﻐﻄﺎء
اﻟﺤﺪﻳﺚ اﻟﻄﺒﻲ
ﺑﺎﻟﻤﺠﻬﺮ ) (Cover glassاﻟﺰﺟﺎﺟﻲ ﻟﻔﺤﺼﻪ
ﻣﺨﺘﺒﺮ
أﺑﻮ ﻣـــــﻮدة
اﻟﻄﺒﻲ اﻟﺤﺪﻳﺚ
اﻟﻄﺒﻲ اﻟﺤﺪﻳﺚ
SOPs
ﻛﻴﻔﻴﺔ ﺗﺤﻀﻴﺮ ﻋﻴﻨﺔ اﻟﻔﺴﺤﺔ
ﺗﺮﻗﻴﻢ ﺷﺮﻳﺤﺔ زﺟﺎﺟﻴﺔ ﻧﻈﻴﻔﺔ
وﺿﻊ ﻧﻘﻄﺔ ﻣﻦ ﻣﺤﻠﻮل اﻟﻤﻠﺢ ) (Normal Saline
ﺑﻮاﺳﻄﺔ اﻟﻌﻮد اﻟﺨﺸﺒﻲ ﻧﺎﺧﺬ ﻣﻘﺪار 1ﺟﻢ ﻣﻦ اﻟﻔﺴﺤﺔ
و ﻧﻤﺰﺟﻬﺎ ﺟﻴﺪا ﻣﻊ ﻣﺤﻠﻮل اﻟﻤﻠﺢ
اﻟﺤﺪﻳﺚ
اﻟﻄﺒﻲ(Cover
وﺿﻊ اﻟﻐﻄﺎء اﻟﺰﺟﺎﺟﻲ ) glass
ﻣﺨﺘﺒﺮ
أﺑﻮ ﻣـــــﻮدة
اﻟﻄﺒﻲ اﻟﺤﺪﻳﺚ
SOPs
اﻟﻄﺒﻲ اﻟﺤﺪﻳﺚ
Name of test Glucose
Company BioMed
Unit mg/dl
Filter 546 nm
•Procedure :
Blank STD Sample
Glucose STD - 10µl -
Sample - - 10µl
Reagent 1.0 ml 1.0 ml 1.0 ml
• Mix and Incubate for 10 minute
•Calculation:
اﻟﻄﺒﻲ اﻟﺤﺪﻳﺚ
A sample × C STD = C sample Conc of STD (100mg/dl)
A STD
•Reference value:
RBG: 80……...….120mg/dl
FBG: 70…….……110mg/dl
2HPPBG: up to 140 mg/dl
ﻣﺨﺘﺒﺮ
أﺑﻮ ﻣـــــﻮدة
اﻟﻄﺒﻲ اﻟﺤﺪﻳﺚ
اﻟﻄﺒﻲ اﻟﺤﺪﻳﺚ
SOPs
ﻛﻴﻔﻴﺔ ﻓﺤﺺ اﻻﺳﺘﻮن ﻓﻲ اﻟﺒﻮل
ﻋﻦ ﻃﺮﻳﻖ اﻟﺸﺮﻳﻂ
ﻏﻤﺮ اﻟﺸﺮﻳﻂ ﻓﻲ ﻋﻴﻨﺔ اﻟﺒﻮل و ﻣﻘﺎرﻧﺔ ﺗﻐﻴﻴﺮ اﻟﻠﻮن ﻓﻲ اﻟﺸﺮﻳﻂ ﻣﻊ
اﻟﻠﻮن اﻟﻤﻮﺟﻮد ﻓﻲ اﻟﻌﻠﺒﺔ
ﻋﻦ ﻃﺮﻳﻖ ﺑﺪرة اﻻﺳﺘﻮن
وﺿﻊ ﻛﻤﻴﺔ ﻣﻦ ﺑﺪرة اﻻﺳﺘﻮن ﻓﻲ ﺷﺮﻳﺤﺔ زﺟﺎﺟﻴﺔ و اﺿﺎﻓﺔ 3ﻧﻘﺎط
اﻟﻄﺒﻲ اﻟﺤﺪﻳﺚ
ﻣﻦ ﻋﻴﻨﺔ اﻟﺒﻮل و ﻣﻼﺣﻈﺔ ﺗﻐﻴﻴﺮ اﻟﻠﻮن ﻟﻠﺒﻨﻔﺴﺠﻲ
ﻣﺨﺘﺒﺮ
أﺑﻮ ﻣـــــﻮدة
اﻟﻄﺒﻲ اﻟﺤﺪﻳﺚ
SOPs
اﻟﻄﺒﻲ اﻟﺤﺪﻳﺚ
Name of test Creatinine
Company BioSystem
Unit mg/dl
Filter 500±20 nm
•Procedure :
• Working reagent 1 ml
• Standard or Sample 100 μl
• Start the stop watch , Mix and insert cuvate into photometer
• Record the absorbance at 500 nm after 30 second (A1) and
90 second (A2)
•Calculation:
(A2 – A1 ) sample × C STD = C sample 2mg/dl
(A2 ـــA1 ) STD
•Reference value:
اﻟﺤﺪﻳﺚ اﻟﻄﺒﻲ
Male: 0.9--------------1.3 mg/dl
Female: 0.6--------------1.1 mg/dl
ﻣﺨﺘﺒﺮ
أﺑﻮ ﻣـــــﻮدة
اﻟﻄﺒﻲ اﻟﺤﺪﻳﺚ
SOPs
اﻟﻄﺒﻲ اﻟﺤﺪﻳﺚ
Name of test Urea
Company BioSystem
Unit mg/dl
Filter 600±20 nm
•Procedure :
Blank STD Sample
Urea STD - 10µl -
Sample - - 10µl
Reagent (A) 1.0 ml 1.0 ml 1.0 ml
• Mix and Incubate for 10 minute at RT or 5 minute at 37c
Reagent (B) 1.0 ml 1.0 ml 1.0 ml
• Mix and Incubate for 10 minute at RT or 5 minute at 37c
•Calculation:
اﻟﻄﺒﻲ اﻟﺤﺪﻳﺚ
A sample × C STD = C sample 50mg/dl
A STD
•Reference value:
• 15-----------45 mg/dl
ﻣﺨﺘﺒﺮ
أﺑﻮ ﻣـــــﻮدة
SOPs
اﻟﻄﺒﻲ اﻟﺤﺪﻳﺚ
اﻟﻄﺒﻲ اﻟﺤﺪﻳﺚ
Cholesterol
Name of test Cholesterol
Company BioSystem
Unit mg/dl
Filter 500nm
•Procedure :
Blank STD Sample
Chol STD - 10µl -
Sample - - 10µl
Reagent (A) 1.0 ml 1.0 ml 1.0 ml
• Mix and Incubate for 10 minute at RT or 5 minute at 37c.
• Color stable for 2 hours.
•Calculation:
A sample × C STD = C sample 50mg/dl
A STD
•Reference value:
اﻟﺤﺪﻳﺚ اﻟﻄﺒﻲ
Up to 200 mg/dl desirable
200---239 mg/dl borderline
More than 240 mg/dl high
ﻣﺨﺘﺒﺮ
أﺑﻮ ﻣـــــﻮدة
اﻟﻄﺒﻲ اﻟﺤﺪﻳﺚ
SOPs
اﻟﻄﺒﻲ اﻟﺤﺪﻳﺚ
Triglycerides
Name of test Triglycerides
Company BioSystem
Unit mg/dl
Filter 500nm
•Procedure :
Blank STD Sample
TG STD - 10µl -
Sample - - 10µl
Reagent (A) 1.0 ml 1.0 ml 1.0 ml
• Mix and Incubate for 10 minute at RT or 5 minute at 37c.
• Color stable for 2 hours.
•Calculation:
A sample × C STD = C sample 50mg/dl
A STD
اﻟﺤﺪﻳﺚ
•Reference اﻟﻄﺒﻲ
value:
Up to 150mg/dl normal
150—199mg/dl borderline
200---499mg/dl high
More than 500mg/dl Very high
ﻣﺨﺘﺒﺮ
أﺑﻮ ﻣـــــﻮدة
اﻟﻄﺒﻲ اﻟﺤﺪﻳﺚ
SOPs
اﻟﻄﺒﻲ اﻟﺤﺪﻳﺚ
Total Protein
Name of test Total Protein
Company BioSystem
Unit mg/dl
Filter 545nm
•Procedure :
Blank STD Sample
Protein STD - 20µl -
Sample - - 20µl
Reagent (A) 1.0 ml 1.0 ml 1.0 ml
• Mix and Incubate for 10 minute at RT.
• Color stable for 2 hours
•Calculation:
A sample × C STD = C sample 50mg/dl
A STD
اﻟﺤﺪﻳﺚ
•Reference اﻟﻄﺒﻲ
value:
6.4--------8.3g/dl.
ﻣﺨﺘﺒﺮ
أﺑﻮ ﻣـــــﻮدة
اﻟﻄﺒﻲ اﻟﺤﺪﻳﺚ
SOPs
اﻟﻄﺒﻲ اﻟﺤﺪﻳﺚ
Albumin
Name of test Albumin
Company BioSystem
Unit mg/dl
Filter 630nm
•Procedure :
Blank STD Sample
Protein STD - 10µl -
Sample - - 10µl
Reagent (A) 1.0 ml 1.0 ml 1.0 ml
• Mix and Incubate for 1 minute at RT.
• Color stable for half an hour.
•Calculation:
A sample × C STD = C sample 50mg/dl
اﻟﺤﺪﻳﺚ اﻟﻄﺒﻲ
A STD
•Reference value:
3.5 ----- 5 mg/dl.
ﻣﺨﺘﺒﺮ
أﺑﻮ ﻣـــــﻮدة
اﻟﻄﺒﻲ اﻟﺤﺪﻳﺚ
SOPs
اﻟﻄﺒﻲ اﻟﺤﺪﻳﺚ
Albumin
Name of test Hemoglobin
Company BioMed
Unit g/dl -- %
Filter 540 nm
•Procedure :
Blank STD Sample
Hemoglobin - 20µl -
STD
Sample - - 20µl
Reagent 5.0 ml 5.0 ml 5.0 ml
• Mix and Incubate for 5minute at RT.
•Calculation:
A sample × C STD = C sample Conc of STD (15 mg/dl -100 %)
A STD
اﻟﺤﺪﻳﺚ اﻟﻄﺒﻲ
Ab of sample × 36.77 = Conc of Test.
•Reference value:
Male: 14.………..…..18 g/dl
Female: 12.…………....16 g/dl
Children: 12……….……16 g/dl
Neonates: 16……………24 g/dl
ﻣﺨﺘﺒﺮ
أﺑﻮ ﻣـــــﻮدة
اﻟﻄﺒﻲ اﻟﺤﺪﻳﺚ
اﻟﻄﺒﻲ اﻟﺤﺪﻳﺚ
SOPs
Giemsa Stain
ﻛﻴﻔﻴﺔ ﺗﺤﻀﻴﺮ ﻣﺤﻠﻮل ﺟﻴﻤﺴﺎ اﻟﻌﻤﻠﻲ •
) :(working reagent
ﻧﺎﺧﺬ 1ﻣﻞ ﻣﻦ اﻟﻤﺤﻠﻮل اﻟﻤﺨﺰوﻧﻲ) 9 +(stock reagentﻣﻞ ﻣﻦ
اﻟﻤﺎء اﻟﻤﻘﻄﺮ ). (DW
ﻃﺮﻳﻘﺔ اﻟﺼﺒﻐﺔ
وﺿﻊ اﻟﺸﺮﻳﺤﺔ ﻋﻠﻰ ﺳﻄﺢ ﻣﺴﺘﻮي ,و ﻏﻤﺮ اﻟﻔﻠﻴﻢ
ﺑﺎﻟﺼﺒﻐﺔ ﻟﻤﺪة 10دﻗﺎﺋﻖ ﺛﻢ ﻏﺴﻞ اﻟﺸﺮﻳﺤﺔ ﺑﺎﻟﻤﺎء
و ﺗﺮﻛﻬﺎ ﺣﺘﻰ ﺗﺠﻒ
اﻟﻄﺒﻲ اﻟﺤﺪﻳﺚ
ﻣﺨﺘﺒﺮ
أﺑﻮ ﻣـــــﻮدة
اﻟﻄﺒﻲ اﻟﺤﺪﻳﺚ
اﻟﻄﺒﻲ اﻟﺤﺪﻳﺚ
Abs g/dl % # Abs g/dl %
0.10 3.7 25% # 0.29 10.7 72%
0.11 4 27% # 0.30 11 74%
0.12 4.4 29% # 0.31 11.4 76%
0.13 4.8 32% # 0.32 11.6 78%
0.14 5.1 34% # 0.33 12.1 81%
0.15 5.5 37% # 0.34 12.4 83%
0.16 5.9 39% # 0.35 12.8 86%
0.17 6.2 42% # 0.36 13.2 88%
0.18 6.6 44% # 0.37 13.6 91%
0.19 7 7% # 0.38 14 94%
0.20 7.5 50% # 0.39 14.3 96%
0.21 7.7 اﻟﻄﺒﻲ اﻟﺤﺪﻳﺚ
52% # 0.40 14.7 98%
0.22 8.1 55% # 0.41 15.1 101%
0.23 8.5 57% # 0.42 15.4 103%
0.24 8.8 59% # 0.43 15.8 106%
0.25 9.2 62% # 0.44 16.2 108%
0.26 9.6 64% # 0.45 16.5 111%
