Univer sit y of Peradeniya
Postgraduate Institute of Science
PROCEEDINGS
PGIS Research Congress
2023
Volume 10
THEME IV Physical Sciences
Abstract Title and Authors Page
ID No. No.
8 DEVELOPMENT OF AN ELECTRIC VEHICLE FOR THE DIFFERENTLY 114
ABLED WITH SOLAR ASSIST
E.S. Mohamed, K. Kaviraj and T. S. Niroshan
18 TITANIUM / TITANIUM DIOXIDE SCHOTTKY JUNCTION AND 115
HIGHLY PHOTOACTIVE ANTIMONY SULPHIDE PHOTOANODE FOR
SOLAR WATER SPLITTING
C.N. Wijerathna and J. Bandara
23 A NANO GRAVIMETRIC APPROACH TO STUDY THE 116
PHOTOCATALYTIC ACTIVITY OF NANO TITANIA THIN FILMS
K.R.J. Manuda, N.L. Fernando, B. Nissanka, A. Tillekaratne and D.R.
Jayasundara
24 ASSESSMENT OF CYTOTOXICITY OF SELECTED EDIBLE LEAFY 117
PLANTS OF SRI LANKA
B.S.K. Ulpathakumbura, J.M.N. Marikkar and U.L.B. Jayasinghe
31 DETERMINATION OF BRINE SHRIMP LETHALITY OF DIFFERENT 118
SOLVENT EXTRACTS OF SOFT STEMS OF DIFFERENT BANANA
(MUSA SP.) CULTIVARS
L.R.M.K.N. Liyadipitiya, J.M.N. Marikkar and U.L.B. Jayasinghe
48 REMOVAL OF MAGNESIUM IONS BY FUNCTIONALIZED CARBON 119
NANOTUBE
A.B. Kirushnah and S. Sivanayani
54 MODEL VALIDATION OF Cu(Ⅱ)- KAOLINITE SYSTEMS BY IN-SITU 120
MEASUREMENT OF FREE METAL ION ACTIVITIES
N. Mudannayake, K. Heenkenda and R. Weerasooriya
56 MULTI-TARGET INHIBITORY ACTIVITY OF SALICORNIA BRACHIATA 121
AND SUAEDA MARITIMA SEED ON α-AMYLASE, TYROSINASE AND
LIPASE
H.M.N.P. Herath, D. Perera, L. Jayasinghe, R.N. Attanayake and P.A.
Paranagama
69 DEVELOPMENT OF HUMIDITY SENSING COMPOSITE MATERIAL 122
USING SnO2 DOPED PHENOL FORMALDEHYDE (PF) DERIVED
CONDUCTIVE CHARCOAL
G.D.K. Heshan, M.D. Madhuwantha, R.M.G. Rajapakse and W.P.S.L.
Wijesinghe
77 CHARACTERIZATION OF SEED OILS OF FOUR GARCINIA SPECIES TO 123
ASSESS POTENTIAL APPLICATIONS IN COSMETICS AND DIETARY
SUPPLEMENTS
K.A.H. Thathsara and S.D.M. Chinthaka
xxx
Proceedings of the Postgraduate Institute of Science Research Congress, Sri Lanka: 3rd-4th November 2023
Abstract No: 56 Physical sciences
MULTI-TARGET INHIBITORY ACTIVITY OF SALICORNIA BRACHIATA AND
SUAEDA MARITIMA SEED ON α-AMYLASE, TYROSINASE AND LIPASE
H.M.N.P. Herath1, D. Perera2, L. Jayasinghe3, R.N. Attanayake4 and P.A. Paranagama1*
1
Department of Chemistry, Faculty of Science, University of Kelaniya, Sri Lanka
2
Department of Bioprocess Technology, Faculty of Technology, Rajarata University of Sri Lanka,
Mihintale, Sri Lanka
3
National Institute of Fundamental Studies, Hanthana Road, Kandy, Sri Lanka
4
Department of Plant and Molecular Biology, Faculty of Science, University of Kelaniya, Sri Lanka
*
[email protected]Diabetes mellitus is a complex metabolic disorder that involves enzymes like α-amylase,
tyrosinase, and lipase. Since currently used antidiabetic drugs have undesirable
contraindications, plant metabolites, particularly seed-based metabolites, has gained special
attention in scientific research as a promising source of enzyme inhibitors. With previous
reports of promising activities of Salicornia brachiata and Suaeda maritima, this study aimed
to explore the enzyme inhibition potential of extracts derived from the seeds of these two
halophytic plant species. The seeds were sequentially extracted using hexane, dichloromethane
(DCM), and methanol by cold maceration and tested for in-vitro inhibitory activity against α-
amylase, tyrosinase, and lipase. The extracts of S. brachiata and S. maritima seeds effectively
inhibited α-amylase activity by 52-83% and 18-73%, respectively, at 0.2 mg mL-1. The hexane
fraction of S. brachiata showed 83.50 ± 1.09% (at 0.2 mg mL-1) inhibition activity that was not
significantly different from the inhibition activity of acarbose, the positive control (at 0.2 mg
mL-1, 84.23 ± 0.88%; p=0.05). It is reported that inhibiting this enzyme slows carbohydrate
digestion, lowering postprandial glucose levels. DCM fraction of S. brachiata and S. maritima
seeds showed tyrosinase inhibitory activity of 57.90 ± 0.84% and 17.80 ± 0.94%, respectively,
at 0.2 mg mL-1, whereas Kojic acid, the positive control had an inhibitory activity of 86.00 ±
0.43% (at 0.2 mg mL-1). Since tyrosinase inhibitors are directly involved with melanin
biosynthesis, the findings are useful in managing hyperpigmentation caused by oxidative stress
in diabetic patients. Moderate inhibition of lipase was observed in the hexane and methanol
extracts of S. brachiata seeds (33-38%, at 0.4 mg mL-1), while the DCM fraction showed
activity of 54.39 ± 3.40% at 0.4 mg mL-1, comparable to orlistat (60.83 ± 0.22%, at 0.4 mg mL-
1
), the positive control. The three extracts from S. maritima seeds exhibited 36-43% (at 0.4 mg
mL-1) inhibition towards lipase, which is important for managing fasting glucose and insulin
levels and reducing the risk of obesity-related type 2 diabetes. The obtained results prompt a
preliminary conclusion towards the potential use of S. brachiata and S. maritima seeds as multi-
target enzyme inhibitors for the modulation of metabolic diseases such as diabetes and
associated complications.
Financial assistance from the STHRD Project, Ministry of Education, Sri Lanka, funded by the
ADB(Grant No CRG/R2/RJ1), is acknowledged.
Keywords: Diabetes, Enzyme inhibitors, Halophyte seeds, Hyperpigmentation, Obesity
disorders
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