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Biserate Analysis and Insights

The document discusses several opportunistic fungal species that can cause disease in humans, including Aspergillus, Fusarium, Geotrichum, Acremonium, Penicillium, Paecilomyces, and Scopulariopsis. It provides details on the microscopic features used to identify each species based on colony morphology on culture media and microscopic structures like hyphae, conidiophores, and conidia. Treatment depends on the infecting fungal species.

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70 views3 pages

Biserate Analysis and Insights

The document discusses several opportunistic fungal species that can cause disease in humans, including Aspergillus, Fusarium, Geotrichum, Acremonium, Penicillium, Paecilomyces, and Scopulariopsis. It provides details on the microscopic features used to identify each species based on colony morphology on culture media and microscopic structures like hyphae, conidiophores, and conidia. Treatment depends on the infecting fungal species.

Uploaded by

michanl
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOCX, PDF, TXT or read online on Scribd

Opportunistic Mycoses Specie Aspergillus Disease Aspergillosis Laboratory Diagnosis Direct microscopic examination of fresh clinical samples; subcultures

should be inoculated onto media that lacks cycloheximide Colonies are usually bluegreen to graygreen in bloodenriched medium Characteristic Feature Treatment/ management

A. Fumigatus

Has a presence of septate hyphae and short or long conidiophores; characterized as foot cell at their base. Uniserate or biserate or both with phialides covering the entire surface of a spherical vesicle Biserate with phialides covering the entire surface of a spherical vesicle; conidia are black Biserate with phialides covering the entire surface of a hemispherical vesicle; aleurioconidia are formed on submerged hyphae Hyphae are small and septate; phialides produce single-celled microconidia or large multicelled macroconidia that are

A. flavus

Colonies are yellow green in blood-enriched medium

A. niger

Colonies are black in bloodenriched medium

A. terreus

Resemble powdered cinnamon in blood-enriched medium

Fusarium

Fusariosis

The most common medium used to induce sporulation is cornmeal agar. Keys for

Geotrichum

Geotrichosis

Acremonium

Esophagitis

Penicillium

Allergic bronchopulmona ry penicilliosis,

identification of species are based on growth on potato dextrose agar Colonies initially appears as a white to cream yeastlike colony; some isolates may appear as white powdery molds Colonies are rapidly growing and also may appear yeastlike when initial growth is observed. Mature colonies become white to gray to rose or reddish orange in color. Colonies are most commonly shades of green or blue-green but pink or white colonies may be seen. Surface of the colony may be velvety or powdery. Colonies are often velvety, tan to olive brown and somewhat powdery.

sickle or boat-shaped

Hyphae are septate and produce numerous rectangular to cylindrical to barrel-shape arthroconidia

Small septate hyphae that produce single, unbranched, tubelike phialides are observed. Phialides gives rise to clusters of elliptical, singlecelled conidia contained in a gelatinous cluster at the tip of the phialide Hyphae are hyaline and septate and produce brushlike conidiophores.

Paecilomyces

Paecilomycosis

Scopulariopsis

Colonies initially appear white but later become light brown and powdery in appearance

Microscopically it resembles a penicillium. However the phialides of Paecilomyces are long,delicate and tapering. Conidia are produced from annelophores and may also be produced singly directly from the hyphae.

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