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Belur Sramajibi Swasthya Prakalpa Samity
ra
Ap 3" Year BMLT Internal Examination- 2023
vubject —Clinical Immunology, Mycobacteriology and Applied Microbiology
St Paper code- 302
‘ull Marks- 100, Time- 3 hours:
A. Tick the correct answer: 20x1= 20
Z. which of the following cells is involved in antibody-mediated immunity:
i) dendritic cells ii) Beells ii) T-helper cells _iv) Monocyte
A, which the following statements is true about hapten?
i) It induces immune response ii) It needs carrier to induce immune response
Itis a T- independent antigen iv) It has no association with MHC
A. Which of the following statements is true about the Ig E molecule
i) IgE is pentamer if)It does not have J chain aav0- o
iii) It protects the mucosal surface iv) Iterosses placenta
Which one is the most sensitive:
i) Precipitation reaction in fl !
iii) Radio immune assay
J. Characteristics of primary
i) Involves IgG
iii) Slow and sluggish.
Acwat is the time taken by the
ii) 1 hr, 15 mins
i615 ming WO Direot agglutination tent where Staph. aureus wet as a carrier molecule:
Aid) Passive or indirect agututination test where Staph. aureus aets as a carrier molecule
iv) Passive agglutination test where latex agglutination test is applied
a Which statement is falye about complement fixation test:
4) Itis a pwo>step reaction
46 Botore starting the test, serum is inactivated at 56°C for 30 mins
iii) I includes complement fixation with a hemolysis indicator system,
iv) uses OX RBCs
10In ELISA test, which combination is right for enzyme, substrate and chromogen
respectively?
~)) Horseradish Peroxidase -H30;- Tetramethyl benzidine
ii) Alkaline phosphatase-pNPP-ONPG =~ mM
iv) Antibodies in HIV, Herpes simplex virus
_ A erinciple of antigen detection by Immunoehro
4) The test system consists of a NCM and an absorbent pad,
ii) The NCM in coated at two places in the form of lines +a test iii)The test line is coated with monoclonal antibody against the targeted antigen
4 The control line is coated with antisheep antibody
AS. A flow-through assay consists of all except:
i) Protein A is used for labeling antibody
ii) The sample flows vertically though NCM
iii) Protein - A conjugatebinds to the Fab portion of antibody.
(1 Soyo (1 poye> st AydesBovewono ut soded ayy uo waned 9 é
