HISTOPATHOLOGY 12.

what substance does acid fast bacteria

produce to produce that makes them
1.Which reagent is used for elastic fibers: impermeable to staining:
- Varhoeps - Mycolic acid

2.Goal of fixation: 13. How do we fix hollowed organs?

- Preserve cellular morphology Dilate with soaked n fixatives

3. Failure to form ribbons is due to: 14. nuclear fixatives, except:

- Knife is tilted too much (reduce the tilt) - Flemming's fluid
- Carnoy's fluid
4. Non-coagulant fixatives EXCEPT: - Bouin's fluid
- Osmic acid (All aldehydes are - Newcomer's fluid
non-coagulant fixatives) - Heidenhain's Susa
- ***Starts with letter C or D yung di
5. Causes brown pigment: kasama yata (?)
- Alkaline picrate: Picric acid + 95% Ethyl
Alcohol 15. optimal freezing temperature of skin
- -13C - -16C

6. I. 1st morning urine II. Catheterize 16. Analyze the statements

specimen for I. Chemical reagents can be placed above
female eye level given that it is tightly capped
- Both are incorrect II. Reagents should be placed in a plastic or
plastic covered containers
7.I. Add water to acid II. Undiluted - Both are incorrect
hypochlorite corrosive to steel
- 1st statement is incorrect, 2nd 17. Analyze the statements
statement is correct I. Gram positive bacteria has a thick
peptidoglycan wall and has a
8.Alum haematoxylin alcoholic iodine: lipopolysaccharide
- Cole’s II. Gram negative bacteria has very little
peptidoglycan and not lipid rich
9.Best fixative for IHC and FISH - I is incorrect; II is correct
- 10% neutral buffer
18. Definite sequence
10.Tissue mushy in embedding -Progressive stain

11. automatic tissue processor performed 19. Factors affecting fixation except - none
fixation at which temperature? of the above
- 37-45C
20. Every chemical countainer should
contain - (Date made, Expiration date, name
of the manufacturer and hazard) - ALL of
the above 31. incorrect about osmium tetroxide as
fixative
21. Contractile intermediate filament I. denatures protein by oxidation
protein II. primary fixative for EM
• Actin III. avoided when fat is to be demonstrated
• Vimentin IV. can readily fix mucosa
• Desmin - II and IV
• Neurofilament
32. fault: clearing agent turns milky as the
22. Used for rickettsiae & nissl tissue is placed in.
• Orths;carnoys
- incomplete dehydration
- prolonged fixation
23. Screening marker for lymphoma
- insufficient clearing
• CD45
- overheated paraffin
• CD15
• CD30 33. maximum effectiveness of fixative
• CD19 - 10
- 20
24. Dezenkerization process -5
- 50
25. Fixative for CNS
• 10%NBF 34. micro chatters around the edges of
• 10%Formol saline small tissue biopsy specimen
• Heidenhain's susa - under dehydration
• Zenker formol - excessive dehydration
- inadequate processing
26. Which of the ff. is carcinogenic - open processors dry up the tissue
- Chloroform accidentally

27. what is the best time for the tissue to be 35. What is the composition of Karnovsky’s
put in fixative after surgery (parang ganun Fluid
nadin) - 2% Paraformaldehyde + 2.5%
- 20-30 minutes Glutaralfehyde

28. Type of silution in dessication process 36. Papaniculaou stain: cytoplasmic stains
- isotonic salt solution I. OG-6 is a polychrome synthetic stain
II. EA-36 is a monochrome
29. vapor fixative except
- acetaldehyde Choices:
- I is correct II is incorrect
30. Low molecular weight calcium binding - II is correct I is incorrect
protein - S-100 - Both are correct
- Both are incorrect
48. Recommended volume of osmium
37. What is the optimal temperature of tetroxide
thyroid? - 5-10
- (-10 to -13 degree C) / -5C to -15C
49.Optimal freezing point of testicular
38. __________ removes not only target -10 to 13 degrees
cells but also the small portions of the
surrounding tissue 50. Incorrect of the ff.
- core needle biopsy I. Additive fixative
II. Less conc soln. Will Causes shrinkage
39. optical tempt of rectal cells
-13 to 16 III. Destroys hydrogen bonds
IV. Best used at ref temp.
40. physical method of fixation
- Pricking with teasing needle 52. Causes swelling but can be used to
counteract shrinkage
41. causes of scratches, line and split a. Phenol
- Dirty knife/embedding (damage on b. Acetone
knife edge) / Tilt is too great c. Acetic acid

42. presence of mucus, blood and fat 53. I. A delay in transporting body effusions,
- Wash with NSS Formalin can be used as an alternative
II. A delay in processing of CSF is
43. an iron hematoxylin which to unavoidable, equal parts of ethyl alcohol is
demonstrate mycelin recommended
- Baba/taas ng verhoeps haha a. Both true
b. Both false
44. the first and crucial processing c. 1st true, 2nd false
-Fixation d. 1st false, 2nd true

45. optimal freezing temp of spleen 54. Causes holes in tissue section
- -5C to -15C a. Block placed aggressively
b. Incomplete decalcification
46. fault sections adhere to knife can be c. Small flecks of tissue samples are
remedy by…. removed
- Breathe out or blow gently on the d. All of the above
bock and knife to break up static
electricity, or boil water in the room 59.Mature bone cell that resides inside the
to increase humidity lacunae
- - Chrondrocytes
47. Gram stain is what type of staining
techniques 60.Study of Inorganic constituent, pigments
-Differential staining - Both of these
61. type of epithelial cell wherein the heights 69. color of RBC when stained with
and width of the cell is approximately equal Heidenhain’s iron hematoxylin
- Cuboidal - Black (blue-black)
- Pink
62.Tendency of tissue to crack maybe - Red
prevented using plasticizers using ___ when - Blue
embedding with chrome-mordanted tissue
- oleum ricini or castor oil 70. Hematoidin is chemically same with
bilirubin and can be demonstrated by
63. Type of honing used for manual - Hall’s method
sharpening - Hallie’s method
- Belgium yellow - Helly's method
- Halley' method
64. Type of honing that gives more
polishing effect 71. Essential oil derived from plants and a
- arkansas substitute for xylene
- Terpenes
65. Temperature of dry oven in hot plate - Aniline oil
- 5-10 degrees below the melting point of - Cedarwood oil
the paraffin wax - Benzene

66. The spread of malignant neoplasm from Candidiasis

its site of origin to a remote site, usually
through blood and lymphatic vessels 72. Used as a nitrocellulose for double
A. Chemotaxis celloidin technique
B. Metastasis A. Benzene
C.Osteomalacia B. Chloroform
D. Parakeratosis C. Amyl acetate
D. CCl4
67. The concentration of the best all around
decalcifier is 73. A good substitute for cedarwood oil in
A. 5% manual tissue processing
B. 10% A. Limonine
C. 15% B. Cedarwood oil
D. 20% C. Terpine
D. Terpineol
68.It stains vividly with acid ( anionic dye)
such as eosin after formalin fixation but is 74. Clearing time for benzene
poorly stained after "SuSa" fixation A. 15-30 mins (15-60 mins)
A. Hematozoin B. 30 mins-1hr
B. Hemoglobin C. 30-45 mins
C. Hematoidin D. 1-2hr
D. Hemosiderin
75. Clearing agent for tough tissues
- chloroform 85.Routine staining for glycogen uses the ff
fixatives except.
76. Clearing agent for CNS tissues, smooth - acetic acid, alcohol formalin, Bouin's,
muscles, skin = cedarwood oil Brasil's, Kelly's and Gendre's
solution (basta yung wala dyan)
77. How long does it take for cedarwood oil
to clear celloidin 86. Specific test for glycogen
- 2-3 days A. PAS
B. Alcian Blue
78. All are non toxic xylene substitutes D. Fuelgen
except
A. Limonene 87. acid mucopolysaccharides are present
B. 1.7% dish washing solution in the following except
C. Benzene - [ ] wharton's jelly
D. Coconut oil - [ ] synovial fluid
- [ ] vitreous humor
79. Limonene is prepared industrially - [ ] circle of willis
A. Steam inhalation
B. Steam purification 88. useful in detecting encapsulated fungi
C. Steam distillation - [ ] southgates
D. Steam inhalation - [ ] alcian blue
- [ ] PAS
80. Clearing and dewaxing agent - [ ] gomori
A. Aniline oil
B. Cedarwood oil 89. most sensitive and versatile
C. Coconut oil - [ ] Sudan III
D. Bleached palm oil (pwede rin xylene) - [ ] sudan IV
- [ ] sudan black
81. Nitrocellulose in double embedding - [ ] sudan red
- di q gets haha
90. Melanomas and Schwannomas always
82. Stain for glycogen or glycoconjugated stain positive for this Intermediate
- PAS Filamentous Marker
- Vimentin
83.
91. Highly specific marker for myogenic
84. Preferred counter stain color when PAS tumor
is used in determining fungi (samting like
dis) 92. Calcium binding protein… CNS glial
A. Light green cells, histiocytes..
B. Light blue - S100
C. Red
D. Orange 96. Most commonly used chelatin agent
Edta
Na citrate
Heparin
Sequestrene

97.Reagent used in lendrums for tissue

softening
4% phenol
1% HCL
neither
Both

98. Ion exhange resin

Requires daily changine of reagents
Chemical test isbused to determine
endpoint decalcification
1 incorrect, 2 correct
1 correct, 2 incorrect
Both corect
Both incorect

99. Fixes and decals at the same time

-acetone
-trichloroacetic
-dpx
-polyethylene glycol

100. Alternative glycerin jelly with an

refractive index of 1.5
-Apathy’s
-dpx
-canada balsam
- Polyvinyl alcohol