CLED Agar (Cystine Lactose Electrolyte Deficient) Cat.
1016
For the inhibition of Proteus swarming in the cultivation of Gram-positive and Gram-negative urinary tract bacteria.
Practical information
Aplications Categories
Selective isolation Urinary tract pathogens
Industry: Clinical
Principles and uses
CLED Agar (Cystine Lactose Electrolyte Deficient) is a non-selective differential plating medium for the growth and enumeration of urinary tract
microorganisms. Omitting sodium chloride inhibits the Proteus swarming and supports the growth of the vast majority of bacteria causing urinary tract
infections, and is used to differentiate and identify them. The presence of bacterial contaminants like Diphtheroids, Lactobacilli and other microbes
indicate the degree of care taken with the handling of the urine specimen.
The microorganisms which cause infection in the urinary tract are generally abundant and of only one species. E. coli is the organism most frequently
isolated.
Beef Extract and Casein peptone provide nitrogen, vitamins, minerals and amino acids essential for growth. Lactose is the fermentable carbohydrate
providing carbon and energy. L-Cystine is added as a growth supplement for cystine dependent coliforms. Differentiation of lactose fermenters and
lactose non fermenters is achieved using bromothymol blue as a pH indicator. Organisms that ferment lactose will lower the pH and change the color of
the medium from green to yellow. Bacteriological agar is the solidifying agent.
Formula in g/L
Bacteriological agar 15 Bromothymol blue 0,02
Casein peptone 4 Gelatin peptone 4
Lactose 10 L-Cystine 0,128
Beef extract 3
Typical formula g/L * Adjusted and/or supplemented as required to meet performance criteria.
Preparation
Suspend 36 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete
dissolution. Sterilize in autoclave at 121 ºC for 15 minutes. Cool to 50 ºC, mix well and dispense into plates.
Instructions for use
For clinical diagnosis, the type of samples are urine.
- Inoculate on the surface 0,1 ml of the dilution 10^-2 of the urine sample.
- Incubate in aerobic conditions at 35±2 °C for 24-48 hours.
- Reading and interpretation of results.
- Report the number of colonies per ml of urine.
- A count of 100.000 (10^5) CFU/ml or more is an indication of a significant clinical urinary tract infection.
Inspired by knowledge Page 1 of 2 - Revision number 3 - Date 11/11/2021 www.condalab.com
Quality control
Solubility Appareance Color of the dehydrated medium Color of the prepared medium Final pH (25ºC)
w/o rests Fine powder Greenish beige Green 7,3±0,2
Microbiological test
Incubation conditions: (35±2 ºC / 24-48 h)
Microorganisms Specification Characteristic reaction
Klebsiella aerogenes ATCC 13048 Good growth Light yellow-blue medium
Proteus vulgaris ATCC 13315 Good growth (swarming inhibited) Blue-blue green medium
Klebsiella pneumonieae ATCC 13883 Good growth
Enterococcus faecalis ATCC 19433 Good growth Light yellow medium
Escherichia coli ATCC 25922 Good growth Yellow medium
Staphylococcus aureus ATCC 25923 Good growth Light yellow medium
Storage
Temp. Min.:2 ºC
Temp. Max.:25 ºC
Bibliography
Bebis, T. D. J. Med. Lab. Technol, 26-38-41. 1968. Mackey, J. R. and Sandys, G.H. 1965.
B.M.H. 1 1173. Mackey, J.R. and Sandys, G.H. 1966. B.M.H. 1 1173. Guttman, D. and Nailer G.R.E., 1967 B.M.J. 2 343-345.
Inspired by knowledge Page 2 of 2 - Revision number 3 - Date 11/11/2021 www.condalab.com
