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Serology Course Overview and Techniques

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43 views4 pages

Serology Course Overview and Techniques

Important

Uploaded by

Mihretie
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOC, PDF, TXT or read online on Scribd

Course Title: Serology

Course Code: MeLS 433


Credit Hour: 3
Lecture: 2 hours/week
Laboratory: 3 hours/week
Prerequisite Course: MeLS 234
Instructor:

Course objectives:
 List range of tests for the diagnosis of syphilis, streptococcal infections, viral infections,
pregnancy tests
 Discuss factors affecting antigen antibody reactions
 Describe the working principles of pregnancy test, syphilis tests, ASO, C-reactive
protein.
 Explain the collection, preparation, preservation and shipment of serologic specimens
 Recognize laboratory quality control in serology laboratory
 Identify possible factors involved in infectious disease diagnostic test results
 Perform specific and non-specific tests for syphilis
 Prepare dilution of serum specimen
 Demonstrate proficiency in febrile agglutination tests for typhoid and paratyphoid fever,
tests for pregnancy, ASO
 Review HIV test algorithm and hepatitis tests
 Perform infectious mononucleosis; rheumatoid factor and acute phase protein tests.

Course description:
Overview on immune system, defense mechanisms by the body, structure and function of
antibodies, the complement system and immunity to infectious diseases. Review of basic
principles of immunologic and serologic reactions; factors affecting antigen antibody reactions,
serial dilution, Collection, preparation, preservation and shipment of serologic specimens; serial
dilution; zonal reaction, principles and practice of specific and non-specific serologic tests for
syphilis; febrile agglutinins (Widal and Weil-Felix tests); auto-immunity (Rheumatoid factor);
streptococcal infections (Anti - streptolysin O titer (ASO) & C-Reactive protein); viral infections
(Tests for HIV & viral hepatitis) and pregnancy test (HCG test in urine); infectious
mononucleosis, toxoplasmosis and acute phase proteins and other miscellaneous tests.
Course Outline:

Course Outline: yellow = modified and red = removed


1. Overview of basic principles of immunologic and serologic reactions (3hrs)
o Introduction
 Overview on immune system, antibodies & complement system
 Definition of terms
o Recapitulation of immunological Techniques
 Primary, Secondary and Tertiary binding tests …….
o Factors affecting antigen antibody reaction

2. Serological techniques (4hrs)


o Introduction
o Materials Necessary for Basic Serologic Tests
o Collection, preparation, Complement inactivation and preservation of
serological specimens
o Shipment of serological specimens
o Dilution
 Serial dilutions
 Determination of end point and titer
3. Common Serologic Tests for Bacterial and Parasitic Infections (10hrs)
o Syphilis serology (5hrs)
 Introduction
 The stages of acquired syphilis
 Congenital syphilis
 Immunologic Manifestations
 Diagnostic Evaluation
 Dark field Microscopy
 Serologic Tests
o Agglutination Tests for Febrile Diseases (3hrs)
 Salmonella
 Serologic Diagnosis
 Rickettsiaceae
 Serologic Diagnosis
o Serology of Streptolysin O (SLO) and Antistreptolysin O (ASO) (1hrs)
 The Extra cellular Products of Streptococcus pyogenes
 Antistreptolysin O (ASO)
 Tests for Antistreptolysin O
 Antistreptolysin O Titration
o Toxoplasmosis (1hr)
 Serological tests
4. Common Serologic Tests for Viral Infections (8hrs)
o HIV Serology (3hrs)
 HIV Antibody Tests
 HIV Antibody Test Algorithm
 Common HIV Antibody Tests
 Enzyme Linked Immunosorbent Assays (ELISA)
 Rapid Tests
 Western Blot
 Explanation of the Meaning of HIV Antibody Test Result
o Serology of Dengue virus
(1hr)
o Serology of H. pylori
o Serology of Hepatitis Viruses (3hrs)
 Introduction
 Hepatitis A virus (HAV)
 Laboratory Diagnosis
 Hepatitis B virus (HBV)
 Laboratory Diagnosis
 Hepatitis C virus (HCV)
 Laboratory Diagnosis
 Hepatitis G virus (HGV)
 Laboratory Diagnosis
 Hepatitis D virus (HDV)
 Laboratory Diagnosis
 Hepatitis E virus (HEV)
 Laboratory Diagnosis
o Serology of Infectious mononucleosis (IM) (1hr)
5. Serology of Rheumatoid Factor, C-reactive protein, and Human Chorionic
Gonadotrophin Hormone (hCG) tests (5hrs)
o Rheumatoid Factor (RF) (1hr)
 Serologic tests
o Acute-phase reactants/ Acute-phase Proteins (1hr)
 C-reactive protein (CRP)
o Serology of Human Chorionic Gonadotrophin Hormone (hCG) (3hrs)
 Introduction
 Serology of hCG in Urine
 Urine pregnancy tests
 Factors that affect urine pregnancy test
 Urine specimen collection
 method of determining hCG

6. Some Miscellaneous Techniques and Monoclonal Antibody Production (2hrs)


o Miscellaneous techniques
 Introduction to flow cytometry
 Isolation of Lymphocyte Populations
 Instrument and quality control
o Methods of Monoclonal Antibody Production
 Hybridoma Technique
 Recombinant DNA techniques

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