Bio-Rad Laboratories infectious disease
Fungitest™
Micromethod for Broth Dilution Antifungal Susceptibility
Testing of Yeasts
User-Friendly Reliable
• Ready-to-use kit Complies with CLSI guidelines:
Quick to inoculate • RPMI 1640 buffered medium
• One-step procedure • Standardized Inoculum
Easy to interpret • Incubation Temperature
• Colorimetric reading • Incubation Time
infectious disease
Fungitest™
Preparation of the Inoculum
2 colonies well-isolated 100 µl
Culture for 24 to 48 hours. 3 ml of sterile distilled water 1.9 ml of sterile
6
(CandiSelect™ 4, Sabouraud medium ...) Mac Farland 1 opacity 5 3 x 10 CFU/ml distilled water
Inoculation 20 µl
+ + Positive controls
2 - 32 5 - Fluorocytosine
2-8 Amphotericin B
100 µl/well
0.5 - 8 Miconazole
0.5 - 4 Ketoconazole
3 ml of suspension medium Fungitest™ microplate 0.5 - 4 Itraconazole
(10 3 CFU/ml) 8 - 64 Fluconazole
- - Negative controls
Incubation 48 hr at 37°C 72 hr at 32°C
Bio-Rad, S.N.C. au capital de 50 000 000 €, Locataire-Gérant, 449 990 712 RCS Nanterre • N° Intracommunautaire FR 62 449 990 712 • Siret 449 990 712 00019
(cryptococci)
Reading + + Positive controls
and - - Strain inhibited by 5- fluorocytosine
Interpretation
- - Strain inhibited by amphotericin B
Interpretation + - Strain intermediate to miconazole
+ - Strain intermediate to ketoconazole
+ - Strain intermediate to itraconazole
e.g. Strain of C.krusei n°4221 + + Strain non inhibited by fluconazole
- - Negative controls
• Only examine the microplate when the positive control (T+) wells are pink
• Observe any colour change in the wells containing the antifungal agent compared to the negative control wells (blue)
• Interpret according to the colour of the 2 wells for each antifungal agent:
- Blue-Blue = no growth: strain inhibited by the antifungal agent in vitro
- Pink-Blue = low growth: intermediate strain
- Pink-Pink = growth: strain not inhibited by the antifungal agent in vitro
Ordering Information
Catalog No. Description Catalog No. Description
60780 Fungitest™ . . . . . . . . . . 10 microplates 63746 CandiSelect™ 4 . . . . . . . . . . . 20 plates Ø 90 mm
56499 Opacity standard kit 63774 Sabouraud + Cmp + Genta . . 20 plates Ø 90 mm
(Fungitest™, AuxaColor™ 2) . . . . 2 vials 56513 AuxaColor™ 2. . . . . . . . . . . . . . . . 20 microplates
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