PeaceHealth Laboratories
SurePath™ Collection Guide
The SurePath Pap test can be collected with two different methods:
using a cervix-brush or a spatula/cytobrush combination.
Correct collection is critical to ensure accurate test results. If you have
questions about collecting for SurePath testing or would like to request
additional training, please contact your account representative.
Rovers Cervix-Brush® Protocol
Step One Step Three
Collect with brush Label and Send
Obtain an adequate sampling from the Tighten the vial cap so the torque line on the cap
cervix using the Cervix-Brush. Insert passes the torque line on the vial. Label the vial
the central bristles of the brush into with two patient identifiers (first and last name
the endocervical canal deep enough with date-of-birth or laboratory order number),
to allow the shorter bristles to fully physician name and date. Place into a specimen bag
contact the ectocervix. Hold the stem with laboratory order.
between the thumb and forefinger.
Maintain gentle pressure and rotate the Note: The test for HPV may be ordered from the same
brush five times in a clockwise direction. specimen collected in the SurePath vial.
Step Two How to Order Supplies
Drop brush head into Fax supply order form to 866-511-5963 or call
SurePath vial Client Services at 800-826-3616.
Preserve the entire
■■ Supply #125228, SurePath Vial
sample by placing your
gloved thumb against the ■■ Supply #125237, Cervex-Brush
back of the brush pad.
Disconnect the entire brush head from
the stem and drop into the preservative vial.
800-826-3616 www.peacehealthlabs.org
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PeaceHealth Laboratories
SurePath™ Collection Guide (continued)
Pap Perfect® Spatula and Cytobrush® Protocol
Step One Step Four
Collect with Spatula Drop Brush into Vial
Select contoured end of the plastic Visually locate the red
spatula and rotate 360 degrees around notched score line on the
the entire exocervix while maintaining side of the Cytobrush handle,
tight contact with exocervical surface. about 4 cm from the brush
Remove spatula. tip. With glove hand and one
single, quick and firm snap,
Step Two separate the brush head-short
Drop Spatula Head into SurePath Vial handle from the rest of the brush handle.
Visually locate the notched score line on the side Do not touch collection end. Drop brush
of the spatula handle. With gloved hand and one head-short handle into the same preservative vial.
single, quick and firm snap, separate the contoured
end from the rest of the spatula handle. Do not Step Five
touch collection end. Drop this contoured collec- Label and Send
tion end into the preservative vial. Place cap on vial Tighten the vial cap so the torque line on the cap
until step four, but do not tighten. passes the torque line on the vial. Label the vial
with two patient identifiers (first and last name
Step Three with date-of-birth or laboratory order number),
Collect with Brush physician name and date. Place into a specimen bag
Insert brush into the endocervix until only with laboratory order.
the bottom-most bristles remain exposed.
Note: The test for HPV may be ordered from the same
Slowly rotate ¼–½ turn in one direction. To
specimen collected in the SurePath vial.
reduce bleeding, do not over-rotate brush.
Over-rotation may result in poor sample
collection. Remove brush. How to Order Supplies
Fax supply order form to 866-511-5963 or call
Client Services at 800-826-3616.
■■ Supply #125228, SurePath Vial
■■ Supply #125237, Spatula & Brush
800-826-3616 www.peacehealthlabs.org
