Ques

tionbank
S.
[Link]
IEXAMINATI
ON2022-
2023
SUBJECT-MI
CROBIOLOGY

QUESTI
ON:
1:
MCQ

1
) ……………i
sanexampl
eofLi
ghtmi
cros
copy a)Scanni
ng

b)Tr
ans
mis
sion

c)
Dar
kFi
el
d

d)
Ref
lect
ion

2)Anexampl
eofI
ndi
cat
ormedi
um-
---
---
---
- a)MacConkeyAgarmedi
um

b)Nut
ri
entBr
oth

c)Nut
ri
entAgar

d)Cz
apeckdoxmedi
u

a)caps ul e
3)Thechar act er
isti
cs hapeofthebact er
iai
s b)cel lwal l
maintai
nedbecaus eof………… c)
cell membr ane
d)sli
mel ayer
[Link] enmet al
4)IntheElectronmi croscopes ourceof BMer cur y
elect
ronsisfrom [Link] nationofMer curyand
Tungs ten
[Link] cury
5)Preservat
ionofmi croorgani
smsus ing A. St or ageatl owt emperatur
e
vacuumandf r
eez i
ngi sempl oy
edin_ ___
_ B. St or agei nster
il
es oi
l
C. St or agei nmineraloi
l
D. Ly ophi li
zati
on
6)Bacteri
alprotectioni saccomplishedby [Link] mbr i
ae
…………. .
. [Link] e
C. Cy tos kelet
on
D. s por e
7)Branchofs ciencewhi chdealswi t
hs t
udyof a).Bact er iol
ogy
Fungiisknownas_ _____? b).
Virol ogy
c)
. My col ogy
d)Phy col ogy
8)Cidarwoodoi li
sus edwi t
h_____
_ _
__ a.10X
objecti
ve. b.40X
c.100X
d.1000X

9)
Whi
choft
hef
oll
owi
ngi
sthemet
hodf
or a.
Pourpl
ate
pr
eser
vat
ionofbact
eri
alcul
tur
e? [Link]
trogen
[Link]
copi ccount
[Link]

1
0)Vi
rus
esar
ebes
tgr
owni
n__
___
___
___
. [Link]
b. Enri
chedagar
[Link]
entagar
d. Ti
ssuecult
uremedi
a

1
1).
Lami
narai
rfl
owi
sequi
ppedwi
th_
___
___
. [Link] ilt
er
b. Asbestosfi
l
ter
[Link]
tzf
il
ter
[Link]
efil
ter

1
2)Whichoft
hefol
l
owi
ngmedi
umi
sus
edf
or [Link]
entagar
i
sol
ati
onoff
ungi
? b. [Link] um
[Link]’
sagar
[Link]

1
3)Magni
fi
cat
ionofoi
li
mmer
sionobj
ect
iv
eis
-- a)10x
-
---
---
- b)40x
c)1000x
d)1
00x

1
4)……………dev
elopedpur
ecul
tur
etechni
que. a)A.
V. Leeuwenhoek
b)JohnTy ndall
c)Li
ster
d)Rober tKoch
1
5)Whoi
sknownasFat
herofmi
crobi
ol
ogy
? a)[Link]
b)JohnTy ndall
c)Li
ster
d)Rober tKoch

16)Testfors
teri
li
tyi
sint
endedt
odet
ect a)Vi
ablemicroorgani
sms
presenceof___
___ b)Dustpart
icles
c)Par
ti
culat
ei mpuri
ty
d)Chemicalimpuri
ty

1
7)Ther
eproduct
ionr
atei
slessthandeat
h A. st
ationaryphase
r
atei
nwhichphas
eofgrowthcycle? B. Deat hphase
C. Lagphas e
D. Logphas e
1
8)……………..
i
sanexampl
eofel
ect
ron A.
Scanni ng
micr
oscopy B.
phas econtrast
C.
Dar kField
D.
Reflection
19)Opti
mumtemperat
urerequi
redforthe A. 37
growthofmes
ophi
lesis--
---
---
---
---
degree B. 25
celci
us C. 15
 D.27
20)Thef
ull
for
mofHEPAf
il
teri
s--
---
---
---
--- a)Hi
gheff
ectivepar
ti
culateair
b)Highef
fecti
vepart
icl
esi nai
r
c)Highef
fi
ciencyofparti
clesi
nair
d)
Highef
fici
encyparti
culateai
r

21)Medi
aprepar
edbyaddi
ti
onofblood, a)Enri
chedmedi a
serumoreggtobas
almedi
aiscal
l
edas_ __
__ b)Enri
chmentmedi a
c)Sel
ecti
vemedia
d)Dif
ferent
ial
media

22)Whi
chi
ndi
cat
ori
sus
edi
nci
tr
atet
est
? A.
methylr
ed
B.
bromothymolbl
ue
C.
phenolred
D.
malachi
tegreen

23)Pres
ervat
ionofmi
croor
gani
smsus
ing a)St
orageatl
owt emperat
ure
l
iqui
dnit
rogenisempl
oyedi
n___
__ b)St
orageinsteri
lesoi
l
c)Stor
ageinmi neral
oil
d)Lyophi
li
zat
ion

24)
___
___
_ist
hebi
ol
ogi
cal
Indi
cat
orus
edf
or A.
[Link]
Aut
ocl
avi
ng. B.
Bacill
uss ubtil
i
sv arni
ger
C.S.
typhi
[Link]
l
usst earothermophilus.
25)Whichoft
hef
oll
owi
ngi
snonmot
il
e [Link] oteusv ul
gar i
s
bact
eri
a? b. Ps eudomonasaer ugi
nos a
[Link]
apneumoni ae
[Link]

26)Cellwal
lofMycobact
eri
umt
uber
cul
osi
s [Link] i
caci
d
cont
ains___
___
___
_. b. Pepti
caci
d
c.
My coli
caci
d
[Link]
caci
d

27)Kov
ac’
sreagenti
sus
edi
n__
___
__t
est
. [Link]
b. Cit
ri
cacid
c.
VogesPr os
kaur
[Link]
ed

28)Whichofthefoll
owi
ngmet
hodisus
edf
or [Link]
st
eril
i
zati
onofspatul
a? [Link] anef i
ltrat
ion
[Link] cal met hod
[Link] teril
i
zati
on
29)Pri
maryst
ainusedingr
amstai
ni
ngi
s [Link]
al v i
olet
[Link] Fus chin
[Link] afranin
[Link] een
 [Link]
30)Thetemperat
urerequir
edtoki
ll
90%of [Link]
themicr
oorgani
smsinas ampl
eatas peci
fi
c [Link] al
ue
ti
meisthe [Link] al
ue
31)Whofir
stusedant
isepti
cduri
ngsurger
y? a.
[Link] t
eur
[Link]
[Link]
d.
Rober tHooke

32)Whichofthef
oll
owi
ngi
snotachemi
cal [Link]
di
si
nfectant
? b.
Chlori
ne
[Link]
y
d.
Malachi
tegr
een

33)As
bes
tosf
il
ter
sar
eal
soknownas_
___
_ a)Berkefieldfil
ter
b)Fri
tteredgl assfi
lt
er
c)Sei
tzf il
ter
d)Mort onfil
ter

34)Mor
dantus
edi
ngr
ams
tai
ni
ngi
s__
___ a)Crystalvi
ol
et
b)Gram’siodi
ne
c)Safranin
d)Methylenebl
ue

a)quaternar
yammoni
ums
alt
s
35)Whichofthefol
l
owi ngagentsareusedas b)alcohol
apres
ervat
iveinophthalmi
csoluti
ons? c)detergent
d)al
dehy des

36)Posi
ti
veai
rpr
ess
uremeansai
rfl
owf
rom a)Steadypressure
__
___
___
__ b)Highpr essureareatol
owpr essur
e
area
c)Lowpr essureareatohi
ghpressure
area
d)Si
mil
arpr essur
eatbothsi
de

37)Dett
oli
schemi
cal
l
y__
___cat
egor
y [Link]
di
sinf
ect
ants. [Link] de
[Link]
[Link]
[Link]
c
38)Identifytheter
mt hatcandescri
bea [Link] ost
ati
c
disi
nfectantthatcani
nhibitt
hegrowthof [Link] ci
dal
fungi
: [Link]
40)Fungi ……….
. Aar ephot osyntheti
c
[Link] okaryoticcel
ls
[Link] l
wallsofpepti
dogl
ycan
[Link] eextracell
ul
arenzymes
41)Antibioti
csareeff
ectiv
eagainst___
___
___
. [Link] r
us es
b. Fungi
 c.
Bacter
ia
[Link]
otozoa

42)__
____
___
isamet hodusedf
orper
for
ming [Link]
ectmi croscopi
ccount
micr
obial
assayofanti
bi
oti
cs b. Lyophil
i
z at
ion
c.
Cuppl atemet hod
[Link] hod

a)
prot eins
43)f
ungal
cel
lwal
li
smadeupof b)aminoaci ds
c)
pept idogly can
d)chitinandgl ucan
44)Vi
rus
esr
equi
re_
___
___
___f
orgr
owt
h. [Link] eria
[Link]
[Link] s
[Link]
vingcel ls
[Link] tpar ti
cles
45)Testfors
teri
li
tyi
sint
endedt
odet
ect [Link] t
iculateimpur i
ty
presenceof___
___ [Link] emi croorganisms
[Link] cali
mpur it
y

46)_______i
sat echni
queus
edf oris
olati
onofa [Link] atetechnique
bacteri
al cul
ture. b. Lyophili
zation
[Link] f
fusion
[Link] i
odict r
ans fert
oafres
hmedi
um
47)Whi choft hefoll
owi
ngi
nv apourphase a)formal dehy de
di
sinfectant b)s oap
c)dy es
d)detergent
48)Pr i
mar ystainusedi
nAcidfaststai
ni
ngis a)Cr ys t
al v
iolet
b)Car bolFuschi n
c)s afrani
n
d) Malachitegr een

A.0.5-5
49)
___%concent
rat
ionofPhenol
wor
kasa B.5-10
di
si
nfect
ant C.12.5
D1 5-20
50)Sel
ectt
hey
eas
tfr
omt
hef
oll
owi
ng a)Asper gi
llus
b)Sacchar omy ces
c)Streptomy ces
d)Penicil
l
ium

A.
Heatsensiti
vetape
51)
Chemi
cal
Indi
cat
orus
edi
nst
eri
l
isat
ioni
s B.
SporesofBs ubti
li
s
__
____ C.
Bubblepointpressur
etes
t
D.
Masterprocessrecord
52)
Incuppl
atemet
hodt
hegr
aphpl
ott
ed A.
Time
whichrelatesz onediamet
erto--
---
----
---
---
---
- [Link] i
ty
--
----
---
----
-----
-- [Link]
hmoft heconcent
rat
ionof
anti
bioti
cs
[Link]
bacteri
alcapaci
ty
53)Thef ir
s tphaseinbact
eri
algr
owt hcurveis [Link] e
__
_____. b. St at
ionar
yphas e
[Link] inephase
[Link]

54).Thebacteriawhichareabletogrowbes t AThermophi l
i
c
atl
owt emperaturesarecal
led____
_bacteri
a. b. Mesophil
ic
c.
Psychrophil
ic
d.
Thermodur i
c
55).__
____mediumi susedforgrowthoffungi [Link]
dThy ogly
colatemedium
i
nSteril
it
ytes
ting. b..Peptonewat er
[Link]
les d.
Soyabeancasei ndigestmedi
um

56)._
___
__isus
edasas t
andar
dinev
aluat
ion [Link]
orm b.
ofant
imi
crobi
alagent
s Phenol
c.I
s opr
opanol d.
Glutaral
dehyde

57)Whi
chofthef
oll
owi
ngi
sanexampl
eof [Link]
l
usstear
othermophi
l
lus
spor
efor
mingbact
eri
a? [Link]
i
[Link]
eusvul
gari
s
[Link]
nosa

58)Ar
eaofl
ysi
scaus
edbyphagei
sknownas a)pox
b)plaque
c)
pocket
d)pock

59)Vi
taminB12canbeesti
matedand [Link]
ll
uss ps
det
erminedbyusi
ngorgani
sm [Link]
lusLei
chmanni
[Link]
lussubti
li
s
[Link]
60)As ampl
eistakenoutf
romthe a)Fil
tr
ati
on b.
prepar
ati
onandisaddedtothecul
tur
e Dir
ectinoculati
on
mediaunderasepti
ccondi
ti
onsi
sknownas [Link]
on d.
__
_ _
_. Pyrogentest

) Ex
1 pl
ai
nfr
act
ional
ster
il
i
zat
ioni
ndet
ail
6
2).
Wri
teanot
eonMechani
cal
met
hodsofSt
eri
l
izat
ion
6

3)Defi
nedef
erent
ial
stai
ni
ngandwr
it
epr
inci
pl
eandpr
ocedur
eofmonochr
ome
st
aini
ng 6

4)wr
it
eanoteondryheatst
eri
li
zat
ionmet
hodsal
ongwi
thchemi
cal
andbi
ol
ogi
cal
i
ndi
cator
sforthesame.
6

5)Expl
ainAppl
icabil
it
yofI
MViCt est
sinmicr
obi
ologyandcommentonpri
nci
pl
eof
methylr
edandIndoletest 6
6).
Commentonf i
l
trati
onst
eri
l
izati
onwit
hdif
fer
enttypesoff
il
ter
sus
edin
st
eri
li
zati
on 6

7)ExplainPrinci
pleandpr ocedur
einvolv
edi ngr am’sstai
ni
ngandaci d-
fasts
tai
ning
techniques . 6
8)wr i
teanot eonbiologi
calandchemicalindicatorsusedinmoistheatst
eri
li
sati
on
met hods 6
9)Def i
nepr otoplastandwr i
teanoteoncel lwallofgram-posi
ti
vebacteri
a
6
10)Wr i
teanot eonr epr
oducti
oninbacteriaandaddanot eoncaps ul
e
6
11)Des cri
beindet ai
lmeas ur
ementofgrowt hofbact eri
a.
6
12)Wr i
t eindetail
aboutlysogeni
ccyclealongwi ththediagram
6

1
3Expl
ai
nsexual
repr
oduct
ioni
nfungi
usi
nganeatl
abel
l
eddi
agr
am
6

14Di ff
er enti
atebet weengr ampos it
iveandnegat i
vecellwall
.
6
15 Def ines poreandexpl ainbacterialsporulati
on
6
16Def ineSt eri
li
[Link] ainMoi stheatasaphy sicals
teri
li
zingagentwi
thmodeof
action,appl i
cati
ons ,biologicalindi
cat or.
6
17Def i
neMagni f
icati
on. Expl ai
nTEMwi thdiagram.
6
18Enl i
s tdiff
erentbiochemi cal t
estforidentif
icati
onofbact er
ia.
6
[Link] wo t es tforident i
fi
cat i
onofE. col
i.
6
20). Def i
neMi crobiological [Link]
indri
cal
platemet hodandt ur bidi
met ricassaymet hod. 6

21
)Expl
ai
ndi
ff
erentmet
hodsf
ori
sol
ati
onofv
irus
es. 6
22)Wri
teashortnot
eondi
ff
erentmet
hodsf
orpr
eser
vat
ionof
mi
crobi
alcul
tur
e. 6

23)Def
inemedi
[Link]
cri
beSel
ect
iv
eandDi
ff
erent
ial
cul
tur
emedi
a. 6

24)Di
ff
erenti
atebet
weenBact
eri
aandf
ungi
.Namet
womedi
afor
i
sol
ati
onoffungi 6

25)ExplainPur ecul turet echnique. 6

26)ExplainPhenol coef f
ici
enttes tforev aluati
onofant imicr
obialagent s.6
27)Wr i
teanot eonnut r i
ti
onalclassifi
cationofbact eria. 6
28)Enlistvariousmet hodsus edforev aluat i
onofDi si
nfectantasant imicrobi
al
agentsand
explai
nanyonei ndet ail
6
29)Wr i
teindet ai
labouts teri
li
zati
onbyRadi ation
6
30)Des cri
bebact eri
al growthcur vewi ththehel pofneatl abel
leddi agram.
6
31)Explainv ariousmet hodsofpr eservationofbact er i
alcul
tur
e.
6
32)Wr i
teanot eonl yt
iccy cleofv i
rusrepl i
cat i
onanddes cri
behowi tisdiff
erent
fr
oml ysogeni ccy cle
6
33)Expl ai
nas exual met hodsoff ungi repr oducti
onus i
nganeatl abelleddiagram
6
34)Wr i
teanot eonphenol coeffi
cientmet hodandment ionmer i
ts,demer it
sand
appl
icati
ons .6
35)Explainv ari
ousf actorsaf f
ectingact i
onofdi si
nfectant
6
36)Wr i
teanot eonphenol coeffi
cientmet hodandment ionmer i
ts,demer it
sand
appl
icati
ons .6
37)Wr i
teanot eonpr inciple,t
ypesandappl icati
onsoft helaminarairf l
owunit. 6

38)Dis cussmicrobiologi
cal assayofanti
bioti
corv i
tamins.
6
39)Differenti
atebet weenPr okaryoti
ccellandEukar y
ot i
ccel
l
.
Giv etwoexampl esofGr am-posit
iv
ebact eri
aandgr amnegat
iv
e
bact eri
a.
40)Dr awaneatl abell
eddiagr amofT4bact eriophageandaddanoteon
morphol ogyands t
ructur
eofv i
ruses
41)Expl ai
ngas eouss ter
il
i
zingagent swit
hmodeofact ion,
appl i
cati
ons.

42)Cul
ti
vat
ionofani
mal
vir
uses

43)I
sol
ati
onandcul
ti
vat
ionofbact
eri
ophage
44)mor
phologyoff
ungi
45)
Char
acteri
sti
cofi
deal
dis
inf
ect
ant
s