Nutrition
Nutrition
uk
60
2 ORGANISATION
6 PLANT NUTRITION
OF THE ORGANISM
6.1 PHOTOSYNTHESIS
6.2 INVESTIGATING PHOTOSYNTHESIS
6.3 LIMITING FACTORS (EXTENDED ONLY)
6.4 INVESTIGATING GAS EXCHANGE (EXTENDED ONLY)
6.5 LEAF STRUCTURE
6.6 MINERAL REQUIREMENTS
6.1 PHOTOSYNTHESIS
• Green plants make the carbohydrate glucose from the raw materials carbon dioxide and
water
• The reaction requires energy which is obtained by the pigment chlorophyll trapping light
from the Sun
EXAM TIP
If asked for the raw materials required for photosynthesis, the answer is
carbon dioxide and water. Although required for the reaction to take place,
light energy is not a substance and therefore cannot be a raw material.
6 PLANT NUTRITION
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Photosynthesis Theory
• The light energy is converted into chemical energy in the bonds that are holding the
atoms in the glucose molecules together
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• They can also convert it into starch for storage, into lipids for an energy source in seeds,
into cellulose to make cell walls or into amino acids (used to make proteins) when
combined with nitrogen and other mineral ions absorbed by roots
EXAM TIP
The photosynthesis equation is the exact reverse of the aerobic respiration equation
– so if you have learned one you also know the other one!
You will usually get more marks for providing the balanced chemical equation than
the word equation.
6 PLANT NUTRITION
• Although plants make glucose in photosynthesis, leaves cannot be tested for its
presence as the glucose is quickly used, converted into other substances and transported
or stored as starch
• Starch is stored in chloroplasts where photosynthesis occurs so testing a leaf for starch
is a reliable indicator of which parts of the leaf are photosynthesising
• A leaf is dropped in boiling water to kill and break down the cell walls
• The leaf is left for 5-10 minutes in hot ethanol in a boiling tube. This removes the
chlorophyll so colour changes from iodine can be seen more clearly
• The leaf is spread out on a white tile and covered with iodine solution
• In a green leaf, the entire leaf will turn blue-black as photosynthesis is occuring in all
areas of the leaf
• This method can also be used to test whether chlorophyll is needed for photosynthesis by
using a variegated leaf (one that is partially green and partially white)
• The white areas of the leaf contain no chlorophyll and when the leaf is tested only the
areas that contain chlorophyll stain blue-black
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• Care must be taken when carrying out this practical as ethanol is extremely flammable,
so at that stage of the experiment the Bunsen burner should be turned off. The safest way
to heat the ethanol is in an electric water bath rather than using a beaker over a Bunsen
burner with an open flame
6 PLANT NUTRITION
• The same procedure as in the investigation above can be used to investigate if light is
needed for photosynthesis
• Before starting the experiment the plant needs to be destarched by placing in a dark
cupboard for 24 hours
• This ensures that any starch already present in the leaves will be used up and will not
affect the results of the experiment
• Following destarching, a leaf of the plant can be partially covered with aluminium foil
and the plant placed in sunlight for a day
• The leaf can then be removed and tested for starch using iodine
• The area of the leaf that was covered with aluminium foil will remain orange-brown as
it did not receive any sunlight and could not photosynthesise, while the area exposed to
sunlight will turn blue black
• This proves that light is necessary for photosynthesis and the production of starch
• Destarch a plant
• Tie a clear bag containing sodium hydroxide, which will absorb carbon dioxide from the
surrounding air, around one leaf
• Tie a clear bag containing water (control experiment), which will not absorb carbon
dioxide from the surrounding air, around another leaf
• The leaf from the bag containing sodium hydroxide will remain orange brown as it could
not photosynthesise due to lack of carbon dioxide
• The leaf from the control bag containing water should turn blue black as it had all
necessary requirements for photosynthesis
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• As the plant is in water, the oxygen released can be seen as bubbles leaving the cut end of
the pond weed
• The number of bubbles produced over a minute can be counted to record the rate
• The more bubbles produced per minute, the faster the rate of photosynthesis
• A more accurate version of this experiment is to collect the oxygen released in a test tube
inverted over the top of the pondweed over a longer period of time and then measure the
volume of oxygen collected
6 PLANT NUTRITION
• Care must be taken when investigating a condition to keep all other variables constant
in order to ensure a fair test – for example, when investigating changing light intensity, a
glass tank should be placed in between the lamp and the beaker to absorb heat from the
lamp and so avoid changing the temperature of the water as well as the light intensity
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EXAM TIP
Alternative ways of measuring the gas (oxygen) given off in these experiments
would be to measure the volume of gas produced using an inverted measuring
cylinder with graduations filled with water that readings can be taken from as the
water is displaced by the gas, or by using a syringe attached by a delivery tube to
the funnel.
EXTENDED ONLY
• If a plant is given unlimited sunlight, carbon dioxide and water and is at a warm
temperature, the limit on the rate (speed) at which it can photosynthesise is its own ability
to absorb these materials and make them react
• However, most often plants do not have unlimited supplies of their raw materials so their
rate of photosynthesis is limited by whatever factor is the lowest at that time
• There are three main factors which limit the rate of photosynthesis:
• Temperature
• Light intensity
• Carbon dioxide concentration
6 PLANT NUTRITION
Temperature
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Light Intensity
• The more light a plant receives, the faster the rate of photosynthesis
• This trend will continue until some other factor required for photosynthesis prevents
the rate from increasing further because it is now in short supply
At low light intensities, increasing the intensity will initially increase the rate of
photosynthesis. At a certain point, increasing the light intensity stops increasing the [Link]
rate becomes constant regardless of how much light intensity increases as something else is
limiting the rate.
• The factors which could be limiting the rate when the line on the graph is horizontal
include temperature not being high enough or not enough carbon dioxide
6 PLANT NUTRITION
• This means the more carbon dioxide that is present, the faster the reaction can occur
• This trend will continue until some other factor required for photosynthesis prevents the
rate from increasing further because it is now in short supply
• The factors which could be limiting the rate when the line on the graph is horizontal
include temperature not being high enough or not enough light
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6 PLANT NUTRITION
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EXTENDED ONLY
• The knowledge about limiting factors and how they affect the rate of photosynthesis
can be used to help control factors in glass houses to ensure maximum crop yields for
farmers
• Growing crops outside does not allow farmers to control any of these factors to increase
growth of plants
• When considering the use of glasshouses and manipulating conditions like this, farmers
need to balance the extra cost of providing heating, lighting and carbon dioxide against
the increased income
• In tropical countries where temperatures are much hotter, glasshouses may still be used
to control other conditions however they may need to be ventilated to release hot air and
avoid temperatures rising too high, which could cause the denaturation of the enzymes
controlling the photosynthesis reaction
EXAM TIP
Interpreting graphs of limiting factors can be confusing for many students, but it’s quite
simple.
In the section of the graph where the rate is increasing (the line is going up), the limiting
factor is whatever the label on the x axis (the bottom axis) of the graph is.
In the section of the graph where the rate is not increasing (the line is horizontal), the
limiting factor will be something other than what is on the x axis – choose from
temperature, light intensity or carbon dioxide concentration.
6 PLANT NUTRITION
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• Plants are respiring all the time and so plant cells are taking in oxygen and releasing
carbon dioxide as a result of aerobic respiration
• Plants also photosynthesise during daylight hours, for which they need to take in
carbon dioxide and release the oxygen made in photosynthesis
• At night, plants do not photosynthesise but they continue to respire, meaning they
take in oxygen and give out carbon dioxide
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• During the day, especially when the sun is bright, plants are photosynthesising at a
faster rate than they are respiring, so there is a net intake of carbon dioxide and a
net output of oxygen
• We can investigate the effect of light on the net gas exchange in an aquatic plant using
a pH indicator such as hydrogencarbonate indicator
• This is possible because carbon dioxide is an acidic gas when dissolved in water
HIGHEST YELLOW
MORE RESPIRATION
> PHOTOSYNTHESIS
- LOWER pH
(MORE ACID)
HIGHER ORANGE
PHOTOSYNTHESIS
ATMOSPHERIC RED
LEVEL = RESPIRATION
6 PLANT NUTRITION
• Several leaves from the same plant are placed in stoppered boiling tubes containing
some hydrogen carbonate indicator
• The effect of light can then be investigated over a period of a few hours
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6 PLANT NUTRITION
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6 PLANT NUTRITION
ATMOSPHERE
CHLOROPLAST
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STRUCTURE DESCRIPTION
GUARD CELL ABSORBS AND LOSES WATER TO OPEN AND CLOSE THE
STOMATA TO ALLOW CARBON DIOXIDE TO DIFFUSE IN,
OXYGEN TO DIFFUSE OUT
6 PLANT NUTRITION
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FEATURE ADAPTATION
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• Photosynthesis produces carbohydrates, but plants contain many other types of biological
molecule; such as proteins, lipids and nucleic acid (DNA).
• Carbohydrates contain the elements carbon, hydrogen and oxygen but proteins, for
example, contain nitrogen as well (and certain amino acids contain other elements too)
• Other chemicals in plants contain different elements as well, for example chlorophyll
contains magnesium and nitrogen
• This means that without a source of these elements, plants cannot photosynthesise or
grow properly
• Plants obtain these elements in the form of mineral ions actively absorbed from the soil
by root hair cells
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6 PLANT NUTRITION
2 ORGANISATION
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? QUESTION 1
An experiment is set up with four test tubes as shown in the diagram below.
Which test tube would contain the least amount of dissolved carbon dioxide after 6
hours?
? QUESTION 2
Which row of the table below shows the correct effects of deficiencies of essential
minerals for plant growth?
6 PLANT NUTRITION
? QUESTION 3
Which of the following options is the best explanation for the role of chlorophyll in
photosynthesis:
Chlorophyll…
A transfers light energy from the sun because it has magnesium ions.
B transfers chemical potential energy from sunlight into organic molecules which can
be used to synthesise carbohydrates such as glucose.
C transfers light energy from the sun into chemical energy in inorganic molecules
which can be used for growth.
D transfers light energy into chemical potential energy into glucose molecules which
are used in the synthesis of other carbohydrates.
? QUESTION 4
The average number of chloroplasts found in four different types of cell from the leaf
of a plant are shown in the bar chart below.
1 2 3 4
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? QUESTION 5
The diagram below shows how the rate of photosynthesis is affected by different
conditions.
What of the following correctly identifies the limiting factor for photosynthesis at the
three points on the graph?
1 2 3
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2 ORGANISATION
7 HUMAN NUTRITION
OF THE ORGANISM
7.1 DIET
7.2 THE ALIMENTARY CANAL
7.3 MECHANICAL DIGESTION
7.4 CHEMICAL DIGESTION
7.5 ABSORPTION
7.1 DIET
Balanced Diet
• A balanced diet consists of all of the food groups in the correct proportions
• Carbohydrates
• Proteins
• Lipids
• Vitamins
• Minerals
• Dietary Fibre
• Water
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Effects of Malnutrition
• There are different types of malnutrition depending on the cause of the imbalance
• They include:
• Starvation
• Coronary heart disease
• Constipation
• Obesity
CONSTIPATION LACK OF FIBRE IN THE DIET FOOD LACKS BULK FOR MUSCLES TO
PUSH IT THROUGH THE ALIMENTARY
CANAL AND SO RISK OF DISEASES
SUCH AS BOWEL CANCER ARE
INCREASED
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• In many countries in the world, droughts, natural disasters, wars and a poor economy can
lead to mass malnutrition in large areas of the country
• The two types of malnutrition most common in these situations are termed ‘protein
energy malnutrition’ (PEM)and they are:
• Kwashiorkor – caused by a lack of protein in the diet, most common in children under 2.
• Often caused by poverty as high protein foods tend to be more expensive and
scarcer.
• Children suffering from kwashiorkor are always underweight for their age but they
often have a swollen abdomen as their diet may contain a lot of carbohydrate
• Marasmus – the most severe form of PEM, where there is a lack of both protein and
energy in the diet.
• People suffering from this have a much lower body weight than normal and look
emaciated
ACTIVITY LEVELS THE MORE ACTIVE, THE MORE ENERGY REQUIRED FOR MOVEMENT
AS MUSCLES ARE CONTRACTING MORE AND RESPIRING FASTER
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• Ingestion – the taking of substances, e.g. food and drink, into the body through the
mouth
• Mechanical digestion – the breakdown of food into smaller pieces without chemical
change to the food molecules
• Chemical digestion – the breakdown of large, insoluble molecules into small, soluble
molecules
• Absorption – the movement of small food molecules and ions through the wall of the
intestine into the blood
• Assimilation – the movement of digested food molecules into the cells of the body
where they are used, becoming part of the cells
• Egestion – the passing out of food that has not been digested or absorbed, as faeces,
through the anus
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STRUCTURE FUNCTION
SMALL INTESTINE FIRST SECTION IS CALLED THE DUODENUM AND IS WHERE THE
FOOD COMING OUT OF THE STOMACH FINISHES BEING DIGESTED
BY ENZYMES PRODUCED HERE AND ALSO SECRETED FROM THE
PANCREAS
PH OF THE SMALL INTESTINE IS SLIGHTLY ALKALINE - AROUND
PH 8 - 9
SECOND SECTION IS CALLED THE ILEUM AND IS WHERE
ABSORPTION OF DIGESTED FOOD MOLECULES TAKES PLACE
THE ILEUM IS LONG AND LINED WITH VILLI TO INCREASE THE
SURFACE AREA OVER WHICH ABSORPTION CAN TAKE PLACE
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7 HUMAN NUTRITION
OF THE ORGANISM
• Severe diarrhoea can cause the loss of significant amounts of water and ions from the
body, causing the tissues and organs to stop working properly
• There are many causes of diarrhoea, one of which is infection with Vibrio cholerae
bacteria, which causes the disease cholera
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7 HUMAN NUTRITION
• Ingested via infected water or food, if it enters the small intestine it can cause illness in
the following way:
3. The toxin stimulates the cells lining the intestine to release chloride ions from inside
the cells into the lumen of the intestine
4. The chloride ions accumulate in the lumen of the small intestine and lower the
water potential there
5. Once the water potential is lower than that of the cells lining the intestine, water
starts to move out of the cells into the intestine (by osmosis)
6. Large quantities of water are lost from the body in watery faeces
• Mechanical digestion is the breakdown of food into smaller pieces without chemical
change to the food molecules
• It is mainly carried out by the chewing action of the teeth, the churning action of the
stomach and the emulsification of fats by bile in the duodenum
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Types of Teeth
• They are used for chewing to increase the surface area of the food so that it can be
exposed to saliva and other digestive juices and broken down more quickly
• The differing shapes and sizes of teeth enable them to perform slightly different functions:
• Premolars and molars – larger, flat surfaces with ridges at the edges for chewing and
grinding up food
Types of teeth
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Dental Decay
• Many bacteria live in the mouth and most are harmless, however some form a sticky film
with saliva, called plaque, which coats teeth and the areas where they attach to gums
• To begin with, plaque is soft and easy to remove, however if it hardens and forms tartar, it
cannot be removed by brushing
• Tartar around the edges of teeth and gums can allow bacteria to work their way into roots,
causing gum disease and loss of teeth
• If sugar is left in the mouth after eating, bacteria in plaque will feed on it
• The acids gradually dissolve the enamel coating of the teeth, working its way into the
dentine
• Dentine is softer than enamel and so dissolves more easily and quickly
• This is tooth decay and if not dealt with, can cause painful infections and loss of teeth
Dental Health
• Brushing teeth regularly removes the buildup of plaque that can cause gum disease and
removes the sugars in the mouth so bacteria cannot turn them into acids and cause tooth
decay
• Teeth should be brushed with a fluoride toothpaste as this helps to strengthen enamel
and reduce damage from acids
• Regular visits to a dentist ensures that any signs of gum disease or tooth decay can be
dealt with promptly
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• Food is partially digested mechanically (by chewing, churning and emulsification) in order
to break large pieces of food into smaller pieces of food which increases the surface area
for enzymes to work on
• Digestion mainly takes place chemically, where bonds holding the large molecules
together are broken to make smaller and smaller molecules
• Chemical digestion is controlled by enzymes which are produced in different areas of the
digestive system
• There are three main types of digestive enzymes – carbohydrases, proteases and lipases
Carbohydrases: Basics
• Amylases are produced in the mouth and the pancreas (secreted into the duodenum)
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Carbohydrases
• Amylase is secreted into the alimentary canal in the mouth and the duodenum
(from the pancreas) and digests starch to maltose (a disaccharide)
• Maltose is digested by the enzyme maltase into glucose on the membranes of the
epithelium lining the small intestine
7 HUMAN NUTRITION
Proteases: Basics
• Proteases break down proteins into amino acids in the stomach and small intestine
(using an enzyme produced in the pancreas)
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Proteases
Protein digestion takes place in the stomach and duodenum with two main
enzymes produced:
Lipases
• Lipase enzymes are produced in the pancreas and secreted into the duodenum
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• The stomach produces several fluids which together are known as gastric juice
• This kills bacteria in food and gives an acid pH for enzymes to work in the stomach
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• The low pH kills bacteria in food that we have ingested as it denatures the enzymes in
their cells, meaning they cannot carry out any cell reactions to maintain life
• Pepsin, produced in the stomach, is an example of an enzyme which has a very low
optimum pH – around pH 2
• The hydrochloric acid produced in the stomach ensures that conditions in the stomach
remain within the optimum range for pepsin to work at its fastest rate
• Cells in the liver produce bile which is then stored in the gallbladder
7 HUMAN NUTRITION
• It is alkaline to neutralise the hydrochloric acid which comes from the stomach. The
enzymes in the small intestine have a higher (more alkaline) optimum pH than those in the
stomach
• It breaks down large drops of fat into smaller ones. This is known as emulsification. The
larger surface area allows lipase to chemically break down the lipid into glycerol and fatty
acids faster
EXAM TIP
Emulsification is the equivalent of tearing a large piece of paper into smaller pieces of
paper.
7.5 ABSORPTION
• Absorption is the movement of digested food molecules from the digestive system into
the blood (glucose and amino acids) and lymph (fatty acids and glycerol)
• Water is absorbed in both the small intestine and the colon, but most absorption of
water also happens in the small intestine
• Absorption takes place in the second section of the small intestine, the ileum
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• The ileum is adapted for absorption as it is very long and has a highly folded surface
with millions of villi (tiny, finger like projections)
• These adaptations massively increase the surface area of the ileum, allowing absorption
to take place faster and more efficiently
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• Microvilli on the surface of the villus further increase surface area for
faster absorption of nutrients
• Wall of villus is one cell thick meaning that there is only a short
distance for absorption to happen by diffusion and active transport
• Lacteal runs through the centre of the villus to transport fatty acids and
glycerol away from the small intestine in the lymph
EXAM TIP
The way in which the structure of a villus is related to its function comes
up frequently in exam questions.
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7 HUMAN NUTRITION
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? QUESTION 1
A W&Y
B W&Z
C Z only
D W&X
? QUESTION 2
C Taking antibiotics
7 HUMAN NUTRITION
? QUESTION 3
A To emulsify proteins
? QUESTION 4
A Amino acids
B Protein
C Glucose
D Starch
? QUESTION 5
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