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Practical Bioinformatics

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0% found this document useful (0 votes)
18 views28 pages

Practical Bioinformatics

bioinformatics
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF or read online on Scribd
Answer the following questions: 1. Go to Pubmed: [Link] a- Report the number of documents written by Zewail in either Nature or Science journals with the term “femtosecond” eat AND Zowa 1 ( Search text: mio ! : ' \! 44 Bi Number of documents: tc _s”t”:”~—“—~—~—™—™S OP EP EL DIN CUPID PDT E nL Cay Pe TURD Rta a ete cr lary of Medicine btechnology Information “femtosecond” AND Zewail [au] AND (Nature [journal] OR Science [journal]) XX | Advanced Crea t Create RSS Save Email Send to Sorted by: Best match Disp Laser femtochemistry Zewail AH. Science. 1988 Dec 23;242(4886):1645-53. doi: 10,1126/science.242.4886.1645. PMID: 17730575 Zewail [au] AND (Nature [journal] OR Science [journal]) Advanced Save 26 results Create alert Create RSS Email Send to Sorted by: Best Page Four-dimensional electron microscopy. Zewail AH. Science. 2010 Apr 9;328(5975):187-93. doi: 10.1126/science.116613: PMID: 20378810 — Review aia technology Information journal] OR Science [journal]) AND (2004 [dp] OR 2017 [dp]) NOT review [pt] Advanced Create alert Create RSS Email Send to Sorted by: Best match | Displ, LJ Imaging rotational dynamics of nanoparticles in liquid by 4D electro’ 1 microscopy. Fu X, Chen B, Tang J, Hassan MT, Zewail AH. Science. 2017 Feb 3;355(6324):494-498. doi: 10.1126/science.aah3582. PMID: 28154074 Free article. Ultrafast electron crystallography of interfacial wate Lobastov VA, Vigliotti F, Chen S, Zewail AH. jew Text Document - Notepad File Edit View “femtosecond” AND Zewail [au] AND (Nature [journal] OR Science [journal]) Zewail [au] AND (Nature [journal] OR Science [journal]) Zewail [au] AND (Nature [journal] OR Science [journal]) AND (2004 [dp] OR 2017 [dp]) NOT review [pt] | ~ 20perpage~ Sort by Default order + Was this helpful? he = & mouse Mouse) AU016757, Myc2, Niard, Nird, bHLHe39 NP_001170823.1. GI: 293620264 Nucleotide PubMed Taxonomy t Identical Proteins FASTA Senne 034979.3 Gl: 71834865 Nucleotide PubMed Taxonomy |} Proteins FASTA NP_034979.3 FASTA Graphics NP_@34979 454 aa linear ROD 21-MAY-2022 myc proto-oncogene protein isoform a [Mus musculus]. REFSEQ: accession NM @10849.4 ‘musculus (house mouse) 5 Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; jeria; Euarchontoglires; Glires; Rodentia; Myomorpha; -idae; Murinae; Mus; Mus. to 454) oY, Zhou X, Xu T, Liu W and Su X. thic pulmonary fibrosis via oe rio SE SUSHIL, NP_@34979 linear myc proto-oncogene protein isoform a | thus musculus]. __NP_@34979 | [email protected] | REFSEQ: accession NM 010849.4 RefSeq. 's musculus (house mouse) ‘Mus musculus aryota; Metazoa; Chordata; Craniata; Vertebrata; ia; Eutheria; Euarchontoglires; Glires; Rodenti 3 Muridae; Murinae; Mus; Mus. ss 1 to 454) see Y, Zhou X, Xu T, Liu W ane hi bro mere irs arse em rata muscusasag 79 ryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; lia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; idea; Muridae; Murinae; Mus; Mus. 1 (residues 1 to 454) in H, Tang Y, Mao Y, Zhou X, Xu T, Liu W and Su x. -9-Sp-mediated TBPL1 _ Signal 93, 110274 (2¢22) : C-MYC induces idiopathic pulmonary fibrosis via modulation R-9-5p-mediated TEPL1. idues 1 to 454) Lu J, Alencastro F, Roberts A, Fiedor 3, Carroll P, RN, Ranganathan S, Torbenson M, Duncan AW and Prochownik iil Sequence: NP_034979.3 Ew Granhins Hi Open link in new tab Open link in new window Open link in incognito window linear ROD 21-MAY-; usculus]. Save link as... Copy link address Copy Ctrl+C | Copy link to highlight arch Google for "FASTA" Send to: ~ Change region shown gene protein isoform a [Mus musculus] bINP_O34979.3 4 Graphics iprotein isoform a [Mus musculus] PYFECDEEENFYHOOQOSELOPPAPSEDINKKFEL SSTADQLEMMTELLGGDMVNOSFICDPDDETF PARGHSVCSTSSLYLODLTAMASECIOPSWV PEPLVLMEETPPTISSDSEEEQEDEEETDVV Sava ini AAPPSTRKDYPAAKRAK LOSGRVLKQ ERSFFALROQTPELENNEKAPKVVILKKATAYILS Analyze this sequence window k in incognito wine Artic C-M? link to highlight via COBY link to highligh arch Google for “Run BLA’ Ende print induc cMy spect and activation [SignanTransduet) BLAST ® » blastp suite blastn blastx tblastn Standard Protein BLAST tblastx BLASTP programs search protein databases using a proteil Enter Query Sequence Enter accession number(s), gi(s), or FASTA sequence(s) @ clear NP_034979.3 Query subrange @ From = a To OF. upload file Choose File | No file chosen e dob Title Enter a @ title for your BLAST search @ Allin two or more sequences @ Search Set Standard databases (nr etc.) MM Experimental databases | 7 expenmentail | > For more i ¢ | Protein Data Bank proteins(pdb) ui Hs vi) 8 | nism - z fis | a [ human ll {i jO exclude | i i |O) exclude Enter organism common name, binomial, or tax id. Only 20 top taxa will be shown. @ O Models (XM/XP) (_) Non-redundant RefSeq proteins (wp) _)} Uncultured/environn > blastp (protein-protein BLAST) ® PSI-BLAST (Position-Specific Iterated BLAST) PHI-BLAST (Pattern Hit Initiated BLAST) DELTA-BLAST (Domain Enhanced Lookup Time Accelerated BLAST) aBLAST algorithm @ General Parameters | Max target | 500 v | | sequences ae ; Select the maximum number of aligned sequences to display 9 Short queries Automatically adjust parameters for short input sequences @ | Expect threshold . fate-8 10) Toe S| 3v |® Word size | Maxmatchesina {5 oe [Link] says Your organism was not found in the taxonomic lookup. Please, sel valid organism from the suggestions “Standard databases (nr etc.): dha () Experimental database | Protein Data Bank proteins(pdb) ‘human (taxid:9606) protein BLAST) T (Position-Specific Iterated BLAST) F Hit Initiated BLAST) en ane eR Le) select all 15 sequences selected ; _ Scientific Max 7 Description Nana Score |S! me v v sture of apo OmoMYC [Homo sapiens} Homo sapiens ME 4 The crystal structures of Human MYC:MAX bHLHZip complex [Homo sapiens] Homo sapiens 110 1 Homo sapiens 109 1 (Crystal structure of Myc-Max recognizing DNA [Homo sapiens] Homo sapiens 105 10 ‘Chain A. Max protein [Homo sapiens] Homo sapiens 100 10 Homo sapiens 98.7 98. @ Max bHLH domain in complex with 5-carboxyl cytosine DNA [Ho... Homo sapiens 981 98.4 Cognate Dna Through A Dimeric B/hih/z Domain [Homo sapiens] Homo sapiens 97.6 97.6 Homo sapiens 95.6 man Max-Dna Complex: New Insights Into Mechanism.. Homo sapiens 91.4 € in complex with N-Myc [Homo sapiens] plor Promoter [Homo sapiens} Homo sapiens he DNA [Ho.... Scientific Name v Homo sapiens Homo sapiens Homo sapiens Homo sapiens Homo sapiens Homo sapiens Homo sapiens Homo sapiens Homo sapiens Max Score v 118 110 109 105 100 98.7 98.1 97.6 95.6 91.4 Total v 118 110 109 105 100 98.7 98.1 97.6 95.6 Query E Score Cover value s v vw 19% 1e-32 19% 5e-30 18% 9e-30 18% | 2¢e-28 17% 26-26 18% 8e-26 18% 2e-25 18% 3e-25 16% 1e-24 15% 4e-23 91.4 Per. Ident Vv 88.89% 93.10% 92.68% 42.35% 40.74% 40.24% 39.29% 39.29% 40.26% 43.66% | Scientific Query E _ Name “Score Score Cover value mi | vii v Vv ment | | wiht | Homo sapiens ail 18 19% 16-32 Homosapiens 110 110 19% | 5e-30 Homo sapiens 109 109 18% 9e-30 Homo sapiens 105 105 18% 2e-28 Homo sapiens 100 100 17% 2e-26 Homosapiens 98.7 98.7 18% | 8e-26 .. Homo sapiens 98.1 98.1 18% 20-25 ] : Homo sapiens 97.6 97.6 18% 3e-25 mo sz 95.6 95.6 16% | 1e-24 914 15% 46-23 Cee RT Max Total a4 a) 4 iy 93.10% 92.68% 42.35% 40.74% 40.24% 39.29% 39.29% 8 L Scientific Max — Total Name Score Score value Ident v Vv vw Homo sapiens Homo sapiens 93.10% 94 Homo sapiens 92.68% 88 Homo sapiens 42.35% 87 Homo sapiens Homo sapien: .. Homo sapiens Homo sapiens Homo sapiens Wiiindlae diana Local Alignment Local alignment tools find one, or more, alignm aligned. They are can align protein and nucleot Water (EMBOSS) EMBOSS Water uses the Smith-Waterman ; alignment of two sequences. Launch Water tt Open link in new tab Open link in new window Beene (EMBOS i een Save link as... EMBOSS Mate! © nts iilarities b LALIGN applica "*"! Tiibeheleeten! /psdi/emboss water Advanced FASTA + Chain A, PROTEIN (MYOD BHLH DOMAIN) PDB: 1MDY_A GenPept Identical Pi eC Mm sa Mma Te ist nit st AR a SES es Copy S Copy link to highlight Search Google for "> pdb|1MDYIA Chain A, PROTEIN (MYOD BE Print. Inspect zv x |Bzex| Suvix| Smx| oS 1) [Link]/protein/NP_034979 32report=fasta myc proto-oncogene protein isoform a [Mus musculus] NCBI Reference Sequence: NP_034979.3 Identical Proteins Graphics rere sere om en ete) aan HSPLVLKRCHVSTHQHNYAAPPSTRKD) Copy link to highlight MMe aa cag ey ag ta Search Google for ">NP_034979.3 myc proto-oncogene protein isoform aa" Inspect Sa mraz] Enter or paste your first protein sequence in any supported format: |) >pdb|MDYIA Chain A, PROTEIN (MYOD BHLH DOMAIN) |) MELKRKTTNADRRKAATMRERRRLSKVNEAFETLKRSTSSNPNORLPKVEILRNAIRYIEGLOALLRD Input form Web services Help & Documa # Rundate: Wed 25 May 2022 [Link] # Commandlinet water # auto # stdout # asequence emboss_water-120220525-172949-0932- # bsequence emboss_water+120220525-172949-6932- # datafile EBLOSUM62 # gapopen 10.0 # gapextend 0.5 # aformat3 pair # sproteint # sprotein2 # Align format: pair # Report_file: stdout BEA aE aE a ana a Aligned sequences: 2 1: 1MDYA 2: [email protected] Matrix: EBLOSUM62 # Gap_penalty: 10.0 # Extend penalty: 0.5 # # Length: 59 Perea Fey) eee Gs 33/59 (5 # Gaps # Score: 79.0 7 TTNADRRKAATMRERRRLSKVNEAFETLK-RS)

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