Name:______________________________ Section:__________ Date:______ Score:______ 2. Fill in the comparison table below.
Basis of Comparison DNA RNA
Protein Synthesis 1. Number of strands
What are proteins? They are composed of amino acids linked together by peptide bonds. 2. Location in the cell
What are the roles and functions of proteins? 3. Type of sugar
channels in membranes – control the movement of molecules in and out of the cell 4. Nitrogenous base pair
structural molecules – for example, making up hair or muscle in animals
hormones – to regulate the activity of cells Guide Questions:
antibodies – in the immune system Q1. What are the components of the DNA and RNA molecule?
enzymes – to act as catalysts in biological systems. ____________________________________________________________
Q2. What is the structural difference between DNA and RNA?
DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) work together to produce proteins from genetic codes.
Genetic codes are found in DNA or RNA which is made up of nucleotide bases usually in three’s (triplet) that code ____________________________________________________________
for the amino acids making up the proteins. Q3. What nitrogenous base is found in RNA but not in DNA?
____________________________________________________________
Activity 1: Getting to Know the DNA and RNA Structure
Procedure: 1. Read the given information carefully about DNA and RNA Recall!
Directions: Fill in the complimentary DNA strand using DNA base pairing rules. The first three
Component molecules nitrogenous bases were paired already and given as examples.
1. The DNA molecule is composed of
three types of component molecule:
phosphate group, the sugar deoxyribose,
and the bases adenine, thymine, cytosine,
guanine (A, T, C, G).
Nucleotides
2. There are three molecules that form
the basic building block of DNA, the
nucleotides. Each nucleotide is composed Activity 2: DNAmazing Challenge!
of one phosphate group, one sugar There are 3 types of RNA:
molecule, and one of the four bases – in 1. Messenger RNA (mRNA) - transcribes the DNA nucleotide bases to RNA nucleotide bases
the example. Across the strands of the 2. Ribosomal RNA (rRNA) - binds the mRNA and tRNA to ensure that codons are translated correctly
helix, A always pairs with T, and G with C. 3. Transfer RNA (tRNA) - translates the mRNA codons into the correct amino acids
There are two stages of protein synthesis:
Ribonucleic Acid, like DNA, is a nucleic 1. Transcription
acid. However, RNA structure differs from 2. Translation
DNA structure in three ways, shown in
Figure 2. First, RNA is single stranded – To further understand the protein synthesis, let’s talk about genetic code. Genetic codes are found in
whereas DNA is double stranded. Second, DNA or RNA which is made up of nucleotide bases usually in three’s (triplet) that code for the amino
the sugar in RNA is ribose; DNA has acids making up the proteins. Specifically, the DNA genetic codes have mRNA codon counterparts
deoxyribose. Finally, both DNA and RNA determined during transcription. The mRNA codons code for specific amino acids (Table 1).
contain four nitrogenous bases, but
instead of thymine, RNA contains a
similar base called uracil (U). The uracil
pairs with adenine. The major types of
RNA include: messenger RNA (mRNA),
ribosomal RNA (rRNA), and transfer RNA
(tRNA).
Table 1. mRNA Codon Chart ____1. What happens to the DNA during transcription?
A. The DNA unzips. B. The DNA does not unzip.
____2. Which enzyme unzips the DNA?
A. RNA polymerase B. mRNA
____3. What happens after the DNA is unzipped?
A. The mRNA copies the DNA template. B. The mRNA connects the DNA together.
____4. What are “start codons” for? _____________________________
Transcription occurs inside the nucleus, and it is the first step in gene expression. In
transcription stage of protein synthesis, the DNA unzips through the help of enzymes called RNA
polymerases. They combine nucleotides to form an RNA strand (using one of the DNA strands as a
template).
B. Figure 2 shows an mRNA exiting the nucleus. Study the figure to help you answer the following
questions:
Important Codons:
Start Codons: AUG (starts the translation stage);
Stop Codons: UAG, UGA, UAA (stops the translation stage).
When the ribosome reads the start codon, AUG, it will present the codons to the tRNA for
translation stage to begin.
To deepen our understanding about how proteins in our body are made, let’s have an
activity called DNAmazing Challenge!
____5. Why does the newly synthesized mRNA go out of the nucleus after transcription?
Instructions: Study the figures carefully and read the captions very well. Each figure has captions and A. The next phase in protein synthesis occurs in the cytoplasm.
labels. Study and analyze what the figure is all about by reading the captions and the labels. After B. The nucleus cannot accommodate the newly synthesized mRNA.
that, there will be guide questions about the figure. Answer the questions that follow. ____6. What happens after the mRNA goes out of the nucleus?
A. Figure 1 illustrates transcription, which is the first phase in protein synthesis.
A. The codons are translated to produce proteins.
Study Figure 1 to help you answer the following questions:
B. The codons are transcribed from amino acids.
For a protein-coding gene, the messenger RNA carries the information needed to build a
polypeptide. The messenger RNA (mRNA) is the RNA form of the gene that leaves the nucleus
through the nuclear pore and moves to the cytoplasm where proteins are made. Take note,
transcription is the process wherein the DNA sequence of a gene is "rewritten" using RNA
nucleotides. Now, let’s take a look on the next stage which is called translation.
C. Figure 3 depicts the initial stage in translation, which is the second phase of protein synthesis. This
involves the complementary pairing of codons in mRNA with anticodons in tRNA; a process that is
facilitated by the ribosome. Study the figure to help you answer the following questions:
13. What happens as the ribosome moves along the mRNA? ___________________
14. Which covalent chemical bond connects the adjacent amino acids to form a protein or peptide?
____________________________________
Elongation is the second stage in translation where the amino acid chain gets longer. In this
stage, messenger RNA reads one codon at a time. Then, the amino acid corresponding each codon is
added to a growing polypeptide chain. Remember, genetic codes are found in DNA or RNA which is
made up of nucleotide bases usually in three’s (triplet) that code for the amino acids making up the
proteins. Specifically, the DNA genetic codes have mRNA codon counterparts determined during
transcription. The mRNA codons code for specific amino acids. Each time a new codon is read, a
matching transfer RNA gets into the ribosomes carrying the anticodon and the corresponding amino
acid of the mRNA codon. Amino acids are connected with each other by peptide bonds becoming a
7. What are the two subunits of the ribosome? _________________________
polypeptide. During elongation, transfer RNAs move through the A and P sites of the ribosome, as
8. What are the two sites in the large subunit of the ribosome? __________________________
shown above. This process repeats many times as new codons are translated and new amino acids
9. Which part of the large subunit of the ribosome accommodates tRNA?
are added to the polypeptide chain
_______________________ ___10. What do you call the codes that are carried by tRNA? A.
E. Figure 5 shows the termination stage in translation. Study the figure to help you answer the
anticodons B. codons
following questions:
___11. Translation is the process where proteins are synthesized from the codes in the mRNA.
Which molecule is carried by tRNA and corresponds to the codons in mRNA? A. amino acid B.
glucose
___12. Why does complementary pairing of codons and anticodons occur during translation?
A. This ensures that the amino acid carried by the tRNA corresponds to the codons.
B. The pairing allows anticodons to select the codons to be translated.
The second stage of protein synthesis is translation. It is where the codes in the messenger
RNA is translated to a polypeptide that contains a specific series of amino acids. Amino acid bonds
with each other to form polypeptides which make up proteins.
So, let’s take a look on the first stage of translation which is initiation. In this stage of
translation, the ribosome sandwiches the mRNA between its small and large subunit to be
translated. The first codon that will be read is AUG that is called the start codon, and consequently
codes for the amino acid methionine.
D. Figure 4 shows the elongation stage in translation. Study the figure to help you answer the
Termination is the last stage of translation. It is where the finished polypeptide is released.
following questions:
It starts when a stop codon (UAG, UAA, or UGA) is read by the ribosome, activating a series of events
that stop translation and disassemble the subunits of the ribosomal RNA. The polypeptide produced
in this stage will be processed further before it becomes a functional protein.
15. When is translation terminated? ___________________
___16. What is the product of translation phase? A. carbohydrate B. protein
Activity 3: Can you “fill” me
Directions:
Step 1. Fill in the correct mRNA bases by transcribing the bottom DNA strand.
Step 2. Translate the mRNA codons.
____12. Amino acids are held together by ____ bonds.
A. hydrogen B. high energy C. ionic D. peptide
____13. How many codons are needed to specify three amino acids?
A. 3 B. 6 C. 9 D. 12
____14. Which of the following is a similarity between DNA and messenger RNA molecules? They
both contain
A. uracil B. double-stranded polymers C. phosphates D. the same sugar
____15. Events that take place during the synthesis of a specific protein are listed below.
A. Messenger RNA attaches to a ribosome.
B. DNA serves as a template for the production of RNA.
C. Transfer RNA bonds to a specific codon.
D. Amino acids are bonded together.
E. RNA exits from the nucleus to the cytoplasm.
The correct order of these events is
A. B E A C D B. D A E C B C. B C E D A D. C B A E D
Step 3. Write in the anti-codon of the tRNA and the corresponding amino acids.
Step 4. Find the correct amino acid using the Codon Chart.
Assessment
Directions: Read each question carefully. Choose the letter of the correct answer. Write the answer
on the space before each number.
____1. Which molecule carries a copy of the genetic information out of the nucleus?
A. amino acid mRNA B. rRNA C. mRNA D. tRNA
____2. What do you call the sequence of three bases in mRNA that corresponds to an amino acid?
A. amino acid B. code C. anticodon D. codon
____3. What do you call the sequence of three bases in tRNA that corresponds to an amino acids?
A. amino acid B. code C. anticodon D. codon
____4. Where does transcription take place?
A. Golgi bodies B. nucleus C. mitochondria D. ribosome
____5. Which of the following is directly involved in translation phase of protein synthesis?
A. cytoplasm B. nucleus C. mitochondria D. ribosome
____6. Which of the following is carried by the transfer RNA (tRNA)?
A. amino acid B. nucleic acid C. DNA D. ribosome
____7. Which of the following is NOT a part of protein synthesis?
A. elongation B. transcription C. replication D. translation
____8. In the RNA molecule, which nitrogen base is found in place of thymine?
A. cytosine B. thymine C. guanine D. uracil
____9. During the process of transcription, which of the following is produced?
A. ATP B. H2O C. DNA D. mRNA
____10. If the DNA template reads “ATA”, which of the following would be the corresponding mRNA
codon? A. ATA B. UAU C. TUT D. UCU
____11. The genetic code is based upon the reading of how many bases at a time?
A. one B. three C. two D. four
