BOC Study Guide 5th edition Clinical Laboratory Certification Examinations Oversight Editors Patricia A. Tanabe, MPA, MLS(ASCP)“ Director, Examination Activities E. Blair Holladay, PhD, SCT(ASCP)™ Vice President for Scientific Activities, ASCP Executive Director, Board of Certification ‘and the ASCP Board of Certification Staff ® ssi secieey orPublishing Team Erik N Tanck & Tae W Moon (design/production) Joshua Weikersheimer (publishing direction) Notice ‘Trade names for equipment and supplies described are included as suggestions only. In no way does their inclusion constitute an endorsement of preference by the Author or the ASCP. The Author and ASCP urge all readers to read and follow all manufacturers’ instructions and package insert warnings concerning the proper and safe use of produets. the American Society for Clinical Pathology, having exercised appropriate and reasonable effort to research material current as of publication date, does not assume any liability for any loss or damage caused by errors and omissions in this publication, Readers must assume responsibility for complete and thorough research of any hazardous conditions they encounter, as this publication (snot intended to be all-inclusive, and recommendations and regulations change over time, ) dcvcrican Society for Clinical Pathology Press Copyright © 2009 by the American Society for Clinical Pathology. All rights reserved. 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Printed in Hong Kong 1413121110 ‘Tae Board of Certification Study GuideTable of Contents vi Acknowledgments vii Preface ix The Importance of Certification, CMP, Licensure and Qualification xi Preparing for and Taking the BOC Certification Examination 1 Blood Bank Questions 1 Blood Products 8 Blood Group Systems 17 Physiology and Pathophysiology 24 Serology 42 > Transfusion Practice Answers 53 Blood Products 55 Blood Group Systems 59 Physiology and Pathophysiology 62 Serology 69 Transfusion Practice 75 Chemistry Questions 75 Carbohydrates 78 Acid-Base Balance 81 Electrolytes 85 Proteins and Other Nitrogen- Containing Compounds 95 Heme Derivatives 99 Enzymes 104 Lipids and Lipoproteins 107 Endocrinology and Tumor Markers 113 TDM and Toxicology 115 Quality Assessment 117 Laboratory Mathematics 121 Instrumentation Answers 129 Carbohydrates 129 Acid-Base Balance 130 130 132 133 136 137 139 140 141 142 145 145 149 155 166 168 178 187 189 191 194 202 211 211 213 215 216 219 220 221 222 Electrolytes Proteins and Other Nitrogen- Containing Compounds Heme Derivatives Enzymes Lipids and Lipoproteins Endocrinology and Tumor Markers TDM and Toxicology Quality Assessment Laboratory Mathematics Instrumentation Hematology Questions Erythrocytes: Physiology Erythrocytes: Disease States Erythrocytes: Laboratory Determinations Leukocytes: Physiology Leukocytes: Disease States Leukocytes: Laboratory Determinations Platelets: Physiology Platelets: Disease States Platelets: Laboratory Determinations Hemostasis Hematology Laboratory Operations Answers Erythrocytes: Physiology Enythrocytes: Disease States Erythrocytes: Laboratory Determinations Leukocytes: Physiology Leukocytes: Disease States Leukocytes: Laboratory Determinations Platelets: Physiology Platelets: Disease States Platelets: Laboratory Determinations Clinical Laboratory Certification Examinations iiTable of Contents 222 Hemostasis 226 Hematology Laboratory Operations 229 Immunology Questions 229 Autoantibody Evaluation 240 Infectious Disease Serology 248. Protein Analysis 258 Cellular Immunity and Histocompatibility Techniques Answers 265 Autoantibody Evaluation 268. Infectious Disease Serology 271 Protein Analysis 275. Cellular immunity and Histocompatibility Techniques 279 Microbiology Questions 279. Preanalytical and Susceptibility Testing 294 Aerobic Gram-Positive Cocci 300 Gram-Negative Bacilli 313. Aerobic Gram-Negative Cocci B15. Aerobic or Facultative Gram-Positive Bacilli 317 Anaerobes B21 Fungi 328 Mycobacteria 334. Viruses and Other Microorganisms 337 Parasites Answers 345. Preanalytical and Susceptibility Testing 352 Aerobic Gram-Positive Cocci 354 Gram-Negative Bacilli 358 Aerobic Gram-Negative Cocci 359 Aerobic or Facultative Gram-Positive Bacilli WW ‘The Board of Certication Stuy Guide 359 361 363 365 367 369 369 370 374 378 378 379 381 381 383 386 390 399 403 405 413 414 415 417 420 421 423 Anaerobes Fungi Mycobacteria Viruses and Other Microorganisms Parasites Molecular Biology Questions Molecular Science Molecular Techniques Applications of Molecular Testing Answers Molecular Science Molecular Techniques Applications of Molecular Testing Urinalysis and Body Fluids Questions Urinalysis: Pre-Analytical Examination Urinalysis: Physical Examination Urinalysis: Chemical Examination Urinalysis: Microscopic Examination Urinalysis: Complete Examination Urine Physiology Other Body Fluids Answers Urinalysis: Pre-Analytical Examination Urinalysis: Physical Examination Urinalysis: Chemical Examination Urinalysis: Microscopic Examination Urinalysis: Complete Examination Urine Physiology Other Body FluidsTable of Contents 427 Laboratory Operations 427 433 442 445 453 459 462 465 467 472 473 476 478 479 Questions Quality Assessment Safety Management Laboratory Mathematics Instrumentation and General Laboratory Principles Education and Communication Laboratory Information Systems Answers Quality Assessment Safety Management Laboratory Mathematics Instrumentation and General Laboratory Principles Education and Communication Laboratory Information Systems 481 Reading & References Clinical Lokoratory Certification Bxaminations‘Acknowledgments ‘The editors would like to thank Melissa Meeks and Each Miller for theie painstaking efforts in combining and reviewing this body of work in accordance with the ASCP Press and production staff. Special thanks are also extended to all our volunteers (former examination committee members and recently recruited volunteers) fr their commitment in assisting us on this essential resource for laboratory science students and their professors. Thank you to my family - Adarm, Peter and Joe, for their support and understanding during, this project. ~Patricia A. Tanabe, MPA, MLS(ASCP}"M Good luck with your board examtnation—my best to each of you as you embark on an exciting career in laboratory medicine. ~£. Blair Holladay, PRD. SCTIASCPY™ vi The Bontd of Certification Study GuidePreface ‘The Sth edition of the Board of Certification Study Guide for Clinical Laboratory Certification Examinations contains over 2000 multiple choice questions. Unique to this study guide isthe differentiation of questions appropriate for both the Medical Laboratory Technician and Medical Laboratory Scientist levels from questions that are appropriate for the Medical Laboratory Scientist level anly (clearly marked MLS ONLY). The questions in this edition are arranged in chapters which correspond to the major content areas on the examination. Within each chapter, the questions are further grouped by topic. New to this edition are short answer explanations and references for each practice question. ‘Questions with images will appear as they would on the certification examination. Laboratory results will be presented in both conventional and SI units. ‘The practice questions are presented ina format and style similar to the questions included on the Board of Certification certification examinations, Please note: None of these questions will appear on any Board of Certification examination. ‘These practice questions were compiled from previously published materials and submitted questions from recruited reviewers. (Note: These reviewers do not currently serve on any Examination Committee) ‘This book is not a product of the Board of Certification, rather it is a product of the ASCP Press, the independent publishing arm of the American Society for Clinical Pathology. Use of this book does not ensure passing of an examination. The Board of Certifcation’s evaliation and credentialing, processes are entirely independent of this study guide; however, this book should significantly help you prepare for your BOC examination Clinical Laboratory Certification Examinations viiQuestion Editors and Reviewers ‘ur thanks to those who edited? reviewed questions for this book. Blood Bank “Margaret . Pritsraa, MA, MT(ASCP) SBE, tired (o-Bator) armen Atte Pcesoe versity of Alabars 2 irmingham Birmingham. AL ve Joanne Kosanke, MT(ASCP)SBB™ Gotten) Manager immanchematclogy Reference abort american ed Cross Central hie Blood Services Region Columbus, OF Patrica J Blloger, MSBd, MASCE, Muscascr»Ssaao Laboratory Education and Trlning Consus Minneapolis, Minoesota Deborah Firestone, EdD, MT(ASCP) ‘SB ‘sociate Dean ‘Stony Brook Uriversiy Stony Brook. WY (Carol McConnell, MS, MLS(ASCP)™ Taboratry Gordiatcr St Prana Memorial Hospital Sun Franasc,cA Chemistry Polly Cathcart, MMSe, MTVASCPISC, ered ormery, Chemistry Supervisor Piedmont Hospital ‘anna, GA Vico S. Freeman, PRD, FACB, MLS(ASCRIMSC Department Chair ard Daringuished Teaching Profesor Unicity of erat Metical Branch Galeton, TX Ross Molina ABCC), FACE. Medial Dietor, Core Laborer, Einory University Hospital Midtown Assistant Profesor Pathology an Lab Medicine, Emory University School af Medicine tory University sili, GA Christine Papades, PD, MTASCPISC, retired Borer, Professor Pathlegy and tabortory Medicine, Medical Ualversityof South Caalina Chsleston, SC Diane Wileon, PRD, MT(ASCP) Program Diecor Media Technology Morgan Sure University Baltmore, MD PRD, MTASCP), Hematology Donna D Catellone, MS, MT(ASCE)SH (Gaiton) (lca Project Manager Heratclogy Hemodane Siemens Healthcare Diagnostics Tarrytown. WY ‘Sandra Difaleo, MS, MT(ASCP) [aueation Coordinator “Tae Colorado Center for Medic) Laboratoey Science [Link] Kathy W. Jones, MS, MLS(AscP)™ Fredy = Clie Laboratory Science eogram ‘Auburn University Moangrsery Montgomery, AL Linda L. Myers, MEd, MTIASCP)SH [Asutane DrestoeCigalLabortory Se Joseph Medial Center Houston. 1 ohn K. Seariano, PRD, MT(ASCP) ‘Resatane raeosor, Pxtelogy 2 Medicine University of ew Mexico Schaal of Medicine Abaguerque. NM Ruth Scheib, MTCASCP)SH Medial Tchoolost Chevelle CGevelsnd. OF | Immunology Barbata Anne Maier, MEA, MTCASCP)SI retired (Editon) Forme Technical Specs, Trnmunology, Serology & Few Cytometry Geisinger Meal Cemer Dani PA Linda 6 Miler, PRD, StASCE) MB Profesor uf lise Laboratory Seence SUNY Upstate Metal Unive Syracu, NY Kate Rittenhouse-Olzon, PRD, SMascP) restr Dror Biotechnology Program Unwerty a alfa, Te State University sf New ore But, NY Laboratory Operations Ellen Borwell, MBA, MT(ASCP)SH Director of Chica Puthlogy laboratory Operations LUnwerty af Vngns Meal Centar Charowenue. VA Cynthia. Johns, MSA, MLS(ascryesin Se Techni Specialist [atorstony Corporation af America (Gkeand FL Rose J Monaro, PhO, MT(ASCP), DYABCO), PACH Medial Director, Core Laboratory, Emory University Hospital Mldsown ‘satan Professor, fathlony and Lab Medicine, Emory Uriverity Schoo! of Medicine Emory Universiy ‘Alans, GA Patrica A Myers, MTIASCP)[Link] Lead Technslojt, Microbiology (Gastar General Homptal (Gacaater PA Lynn Schwabe, MBA, CHE, MT(ASCP) (GaditorSafeey) Senior Bsetor, Lab Secvices Norshore Univerity Heslthtem Evanston Hosptal Vili The Board of Certification Study Guide ‘Evanston. fo Peggy Simpson, MS, MTVASCP) Aainsrative Director of Laboratories Dale Regional Medial Caner Dale, VA Microbiology ‘Yuet . McCarter, PhD, D(ABMM) (ator Director Clinical Microbiology Laboratory University of Finda Meath Seience {Center-Jacksonile Sksonvile, FL ‘Joan B Fenn, MS, NTCASCP) Profesor and Associate Division ed, ‘Medial Laboratery Scene, Department ‘a Pahclony Uninet of Uah School of Medicine Salas Cay, UT awn , Lampkin, BA, IT(ASCP)[Link] Manayer of Microbiology Services HCA Midiwert Dien, enearch Medic Conner Konan Cig, MO aren Myers, MA, MT{ASCP)SC "Tae Clerc Center fo Metied Laboratory Science Denver,CO atty Newcomb-Gayman, MT(ASCP)SM ant of Care Testing Conedinator ‘Swed Merial Center Sota, WA Molecular Pathology Stephen. Koury, PAD, MT(ASCP) ator Rewareh Asistne Professor Depormeat af Gotcha nd Cline [ab arary Scene, University 3 Blo Bara, 7 Urinalysis and Body Fluids Kristina Jackaon Behan, PRD, MTIASCP) Asocate Profesor snd Progam Director Univer of West Fedo Ciel aboratony Scenes Progra Pensacola, FL Susan Strasinger, DA, MT(ASCP), retired, ore, Visiting Assistant Profesor Unversity of West Pond Pensacola FLCertification, Certification Maintainance Program (CMP), Licensure and Qualification ‘The Importance of Certification, CMP, Licensure and Qualification ‘The practice of modern medicine would be impossible without the tests performed in the laboratory. A highly skilled medical team of pathologists, specialists, laboratory scientists, technologists, and technicians works together to determine the presence or absence of disease and provides valuable data needed to determine the course of treatment. ‘Today's laboratory uses many complex, precision instruments and a variety of automated and electronic equipment. However, the success of the laboratory begins with the laboratorians’ dedication to their profession and willingness to help others. Laboraterians must produce accurste and reliable test results, have an interest in science, and be able to recognize their responsiblity for affecting human lives. Role of the ASCP Board of Certification Founded in 1928 by the American Society of Clinical Pathologists (ASCP—now, the American Society for Clinical Pathology), the Board of Certification is considered the preeminent certification agency in the US and abroad within the field of laboratory medicine. Composed of representatives of professional organizations and the public, the Board's mission is to: “Provide excellence m certification of laboratory professionals on behalf of patients worldwide.” The Board of Certification consists of more than 100 volunteer technologists, technicians, laboratory scientists, physicians, and professional researchers. These volunteers contribute their time and expertise to the Board of Governors and the Examination Committees, They allow the BOC to achieve the goal of excellence in credentialing medical laboratory personnel in the US and abroad. ‘The Board of Governors is the policy-making governing body for the Board of Certification and is composed of 25 members. These 25 members include technologists, technicians, and pathologists nominated by the ASCP and representatives from the general public as well as from the following societies: the American Association for Clinical Chemistry, the AABB, American College of Microbiology, American Society for Clinical Laboratory Science, the American Society of Cytopathology, the American Society of Hematology, the American Association of Pathologists’ Assistants, Association of Genetic Technology, the National Society for Histotechnclogy, and the Clinical Laboratory Management Association (CLMA\. ‘The Examination Committees are responsible for the planning, development, and review of the examination databases: determining the accuracy and relevancy of the test items; confirming, the standards for each examination and performing job or practice analyses, Certification hetp://[Link]/certification Certification is the process by which a nongovernmental agency or association grants recognition ‘of competency to an individual who has met certain predetermined qualifications, as specified by that agency or association. Certification affirms that an individual has demonstrated that he or she possesses the knowledge and skills to perform essential tasks in the medical laboratory. The ASCP Board of Certification certifies those individuals who meet academic and clinical prerequisites and who achieve acceptable performance levels an examinations. In 2004, the ASCP Board of Certification implemented the Certification Maintenance Program (CMP), which mandates participation every 3 years for newly certified individuals in the US. The goal of this program is to demonstrate to the public that laboratory professionals are performing the appropriate and relevant activities to keep current in their practice. Please follow the steps outlined on the website to apply for CMP and retain your certification, ([Link] (Clinical Laboratory Certification Examinations 1xCertification, Certification Maintainance Program (CMP), Licensure and Qualification United States Certification bheepu/[Link]/certification ‘To apply fora Certification Examination follow these step-by-step instructions: 1. Identify the examination you are applying for and determine your eligibility. 2 Gather your required education and experience documentation 2 Apply for the examination. We offer 2 options: a. Apply online and pay by credit card b, Or download an application, pay by ceedit card, check or money order and mailto: ASCP Board of Certification 51335 Eagle Way Chicago. 60678-1039 4 Schedule your examination ata Pearson Professional Center. Visit the Pearson site (htp://wwi. pearsonvue ‘com/ascp to identify alocation and time that is convenient for you to take your ASCP examination. International Certification Iheep://www ascp org/certification/International [ASCP offers its gold standard credentials inthe form of international certification (ASCP?) to eligible individuals. The ASCP’ credential certifies professional competency among new and practicing laboratory personnel in an effort to contribute globally to the highest standards of patient safety Graduates of medical laboratory science programs outside the United States are challenged with content that mirrors the standards of excellence established by the US ASCP exams. The ASCP' Credential carries the weight of 60 years of expertise in clinical laboratory professional certification. Please visit the website to view the following: 1 Website information translated into a specific language 2 Currentlisting af international certifications. 3 Blighility guidelines 4 Step-by-step instructions to apply for international cetification, State Licensure hetp://wwwaascp org/licensure State Licensure is the process by which a state grants a license to an individual to practice their profession in the specified state. The individual must meet the state's licensing requirements, which may include examination and/or experience. It is important to identify the state and examination to determine your eligibility and view the steps for licensure and/or certification. For list of states that require licensure, please goto the website, ([Link] ‘The ASCP Board of Certification (BOC) examinations have been approved for licensure purposes by the states of California and New York. The BOC examinations also meet the requirements for all other states that require licensure. Qualification http//[Link]/qualification ‘A qualification from the Board of Certification recognizes the competence of individuals in specific technical areas. Qualifications are available in laboratory informatics, immunohistochemistry and flow cytometry. To receive this credential, candidates must meet the eligibility requirements land successfully complete an examination (QCYM, QIHC) or a work sample project (QUI). Candidates who complete the Qualification process will receive a Certificate of Qualification, which is valid for S years. The Qualification may be revalidated every 5 years upon receipt of completed application and fee. (Documentation of acceptable continuing education may be requested.) X The Board of Certification Study GuideAbout the Examination Preparing for and Taking the BOC Certification Examination Begin early to prepare for the Certification Examination, Because of the broad range of knowledge and skills tested by the examination, even applicants with college education and those completing formal laboratory education training programs will find that review is necessary, although the exact ‘amount will vary from applicant to applicant. Generally, last-minute cramming is the least effective method for preparing for the examination. The earlier you begin, the more time you will have to prepare; and the more you prepare, the better your chance of successfully passing the examination and scoring well Study for the Test Plan a course of study that allows more time for your weaker areas. Although it is important to study your areas of weakness, be sure to allow enough time to review all areas. It is better to spend a short time studying every day than to spend several hours every week or 2. Setting aside a regular time and, a special place to study will help ensure studying becomes a part of your daily routine, Study Resources heep://[Link] org/studymaterials Competency Statements and Content Guidelines [Link] org/contentguidelines ‘The Board of Certification has developed competency statements and content guidelines to delineate the content and tasks included in its tests. Current Content Guidelines for the Medical Laboratory Scientist (MLS) and Medical Laboratory Technician (MLT) examinations as well as other certification examinations offered by the ASCP BOC are available. Study Guide ‘The questions in this study guide are ina format and style similar to the questions on the Board of Cectfication examinations. The questions are in a multiple choice format with I best answer. Work. through each chapter and answer all the questions as presented, Next, review your answers against the answer key. Review the answer explanation for those questions, that you answered incorrectly. Lastly, each question is referenced if you require further explanation. Textbooks “The references cited in this study guide (see pp 481-484) identify many useful textbooks. The most. current reading lists for most of the examinations are available on the ASCP’s website ([Link] -[Link]/readinglists). Textbooks tend to cover a broad range of knowiedge in a given field. An added benefit is that textbooks frequently have questions at the end of the chapters that you can use to test yourself should you need further clarification on specific subject matter. Online practice tests hetp://[Link] ‘The online practice tes is a subscription product. t includes 90-day online access to the practice tests, comprehensive diagnostic scores, and discussion boards Ifyou are an institutional purchaser that would like to pay by check or purchase order (minimum of 20 tests to use a check or purchase ‘orden, please download the order form from the website. Content-specific online practice tests can be purchased online. Clinical Laboratory Certification Examinations xiAbout the Examination Taking the Certification Examination ‘The ASCP Board of Certification (BOC) uses computer adaptive testing (CAT), which is criterion xeferenced. With CAT, provided you answer the question correctly, the next examination question hhas a slightly higher level of difficulty. The difficulty level of the questions presented to the examinee continues to increase until a question is answered incorrectly. At this point, a slightly easier question is presented. The importance of testing in an adaptive format is that each test is individually tailored to your ability level. Each question in the examination pool is calibrated for difficulty and categorized into a subtest area, which corresponds to the content guideline for a particular examination. The weight (value) given to each question is determined by the level of difficulty. All examinations (with the exception of phlebotomy (PBT) and donor phlebotomy (DPT)) are scheduled for 2 hours and 30 minutes and have 100 questions, The PBT and DPT examinations are scheduled for 2hours and have 80 questions. Your preliminary test results (pass/fail) will appear on the computer screen immediately upon completion of your examination. Detailed examination scores will be mailed within 10 business days, after your examination, provided that the BOC has received all required application documents. Examination results cannot be released by telephone under any circumstances. Your official detailed examination score report will indicate a “pass” or “fail” status and the specific scaled score on the total examination. A scaled score is statistically derived (in part) from the raw score (number of correctly answered questions) and the difficulty level of the questions, Because each examinee has taken an individualized examination, scaled scores are used so that all examinations may be compared on the same scale, The minimum passing score is 400. The highest attainable score is 999, If you were unsuccessful in passing the examination, your scaled scores on each of the subtests will be indicated on the report as well, These subtest scores cannot be calculated to obtain your total score, ‘These scores are provided as a means of demonstrating your areas of strengths and weaknesses in comparison to the minimum pass score, xil The Boeed of certification Study Guide1: Blood Bank | Blood Products Questions Blood Bank ‘The fellowing items have been identified generally as appropriate for both entry level medical laboratory scientists and medica! laboratory technicians. Items that are appropriate for medical laboratory scientists only ‘are marked with an “MLS ONLY” 1 Questione 52 Answers with Explanations 1 Blood Products 53° Blood Products 8 Blood Group Systems 55° Blood Group Systems 17 Physiology and Pathophysiology 59° Physiology and Pathophysiology 24 Serology 62 Serology 42 Transfusion Practice 69. Transfusion Practice Blood Products 1 The minimum hemoglobin concentration in a fingerstick from a male blood donor is: 12.0 g/Ab (120 g/t) b 125 g/l (125 g/L) 135 g/dl (135 g/L) @ 15.0 /aL (1509/1) 2 Acause for permanent deferral of blood donation is: a diabetes residence in an endemic malaria region € history of jaundice of uncertain cause 4 history of therapeutic rabies vaccine Which ofthe following prospective donors would be accepted for donation? ‘a 32-year-old woman who received a transfusion ina complicated delivery § months previously, 1b 19-year-old sailor who has been stateside for 9 months and stopped taking bis anti-inalarial medication 9 months previously ‘© 22-year-old college student who has 2 temperature of 99.2°F (37.3°C) and states that he feels well, but is nervous about donating 4. 45-year-old woman who has just recovered from a bladder infection and is still taking antibioties 4 Which one of the following constitutes permanent rejection status of a donor? 4a tattoo 5 months previously B recent close contact with «patient with viral hepatitis € 2units of blood transfused 4 months previously 4 Confirmed positive test for HBsAg 10 years previously 5, According to AABB standards, which ofthe following donors may be accepted ass blood donor? SS g_traveled toan area endemic for malaria 8 months previously spontaneous abortion at 2 months of pregnancy, 3 months previously resides with a known hepatitis patient received a blood transfusion 22 weeks previously ane (Clinicel Laboratory Certification Examinations 11: Blood Bank | Blood Products Questions Below are the results of the history abtained from a prospective female blood donor age: 16 temperature: 990°F (272°C) Het: 36% isto tetanus toxold immunization 1 week previousy 10 a 12 23 How many of the above results excludes this donor from giving blood for a routine transfusion? bi «2 a3 For apheresis donors who donate platelets more frequently than every 4 weeks, a platelet count ‘must be performed prior to the procedure and be at least: a 150 « 103/ut (150 « 108/t) 1 200% 10%/ut (200 x 10°/1) € 250 x 10/yL (250 x 10°/L) 300 « 10°/al (300 x 10°/1) Prior to blood donation, the intended venipuncture site must be cleaned with a scrub) solution containing: a hypochlorite b isopropyl alcohol © 10% acetone PVP iodine complex All donor blood testing must include: ‘2. complete Rh phenotyping b anti-CMV testing ¢ direct antiglobulin test d serological test for syphilis During the preparation of Platelet Concentrates from Whole Blood, the blood should be: 2 cooled towards 6°C b cooled towards 20°-24°C © warmed to 37°C 4 heated to 57°C “The most common cause of posttransfusion hepatitis can be detected in donors by testing for: a anti-HCV b HBeAg © anti-HAV IgM. 4 anti-HBe ‘The Western blot is a confirmatory test for the presence of: 2 CMV antibody b anti-HIV-1 ¢ HBsAg serum protein abnormalities ‘The test that is currently used to detect donors who are infected with the AIDS vieus is: 2 anti-HBe b anti-HIV 1,2 © HBsAg 4 alr 2 The Board of Certification Stady Guide1: Blood Bank | Blood Products Questions 4 4s 16 7 18 19 20 21 ‘A commonly used screening method for anti-HIV detection is a Intex agglutination by radioimmunoassay (RIA) € thin-layer-chromatography (TC) _ enzyme-labeled immunosorbent assay (ELISA) Rejuvenation of a unit of Red Blood Cells is a method used to: ‘4 remove antibody attached to RBCs b inactivate viruses and bacteria restore 2,3-DPG and ATP to normal levels 4 filter blood clots and other debris Aunit of packed cells is split into 2 aliquots under closed sterile conditions at 8 aM, The expiration time for each aliquot is now: 4 4 PMon the same day 1b BpMon the same day ¢ 8AM thenext morning 4 the original date of the unsplit unit Aunit of Red Blood Cells expiring in 35 days is split into 5 small aliquots using a sterile pediatric ‘quad set and a sterile connecting device. Each aliquot must be labeled as expiring in: a Ghours b iZhours © Sdays 35 days When platelets are stored on a rotator set on an open bench top, the ambient air temperature rust be recorded: 8 once a day B twice a day € every 4 hours @ every hour Which of the following is the correct storage temperature for the component listed? ‘4. Cryoprecipitated AHE, 4°C b Fresh Frozen Plasma (FFP), ~20°C «Red Blood Celis, Frozen, -40°C d Platelets, 37°C A unit of Red Blood Cells is issued at 9:00 am. At 9:10 am the unit is returned to the Blood Bank. ‘The container has mot been entered, but the unit has not been refrigerated during this time span. ‘The best course of action for the technologist is to: ‘& culture the unit for bacterial contamination discard the unit if not used within 24 hours € store the unit at room temperature record the return and place the unit back into inventory ‘The optimum storage temperature for Red Blood Cells, Frozen is: a -80°C b -20°C © -12C a 4c (Clinical Laboretory Certification Examinations 31: Blood Bank | Blood Products Questions 22 The optimum storage temperature for Red Blood Cells a -20°¢ 20°C © -12C a 4c 23 24 25 26 27 28 29 If the seal is entered on a unit of Red Blood Cells stored at 1°C to 6°C, what is the maximum allowable storage period, in hours? as b 24 © 48 a7 ‘The optimum storage temperature for cryoprecipitated AHE is: a -20°C b 12°C e 4C a 27°C Cryoprecipitated AHF must be transfused within what period of time following thawing and pooling? a 4hours b Bhours © 12hours 4 24 hours Platelets prepared in a polyolefin type container, stored at 22°-24°C in 50 ml. of plasma, and gently agitated can be used for up to: a 26hours b 48 hours © Sdays 4 Sdays “The optimum storage temperature for platelets i: 2-20" b -12¢ «4c a 2¢ According to ABB standards, Fresh Frozen Plasma must be infused within what period of time following thawing? a 24hours b 36 hours € 48 hours 4 72hours Cryoprecipitated AHF, if maintained in the frozen state at ~18°C or below, has a shelf life of: 242 days b 6 months ¢ 12 months 436 months 4 The Board of certification Study Guide1: Blood Bank | Blood Products Questions 30 31 32 33 34 35 36 37 38 Once thawed, Fresh Frozen Plasma must be transfused within: a dhours b Bhours © I2hours d 24 hours ‘An important determinant of platelet viability following storage is: 2 plasma potassium concentration b plasma pH € prothrombin time activated partial thromboplastin time In the liquid state, plasma must be stored at a 6 b2ze « 37C asec During storage, the concentration of 2,3-diphosphoglycerate (2,3-DPG) decreases in a unit of a Platelets b Fresh Frozen Plasma € Red Blood Cells 4 Cryoprecipitated AHE Cryoprecipitated AHF: ‘8 is indicated for fibrinogen deficiencies b should be stored at 4°C prior to administration € will not transmit hepatitis B virus is indicated for the treatment of hemophilia B Which apheresis platelets product should be irradiated? 48 sutologous unit collected prior to surgery bb random stock unit going to a patient with DIC «a directed donation given by a mother for her son 4 a directed donation given by an unrelated family friend Ivadiation of a unit of Red Blood Cells is done to prevent the replication of donor: granulocytes b lymphocytes © red cells 4 platelets Plastic bag overwraps are recommended when thawing units of FFP in 37°C water baths because they prevent: 12 the PPP bag from cracking when it contacte the warm water water from slowly dialyzing across the bag membrane € the entry ports from becoming contaminated with water d the label from peeling off as the water circulates in the bath ‘Which of the following blood components must be prepared within 8 hours after phlebotomy? Red Blood Cells Fresh Frozen Plasma Red Blood Cells, Frozen Cryoprecipitated AHF anor Clinical Laboratory Certification Examinations: 51: Blood Bank | Blood Products Questions 39. Cryopreciptated AHE contains how many units of Factor VIL? Be Sao 80 « 130 4-250 40. Which of the following blood components contains the most Factor Vill concentration relative Mi, to volume? 42 Single-Donor Plasma b Cryoprecipitated AHF «Fresh Frozen Plasma 4 Platelets 41 The most effective component to treat a patient with fibrinogen deficiency is: OM a Fresh Frozen Plasma b Platelets Fresh Whole Blood 4 Cryoprecipitated AHF 42 Ablood component prepared by thawing Fresh Frozen Plasma at refrigerator temperature and removing the fluid portion is: a Plasma Protein Fraction b Cryoprecipitated AHF Factor IX Complex a FP24 43. Upon inspection, a unit of platelets is noted to have visible clots, but otherwise appears normal. ‘The technologist should a issue without concern bb filter to remove the lots € centrifuge to express off the clots 4 quarantine for Gram stain and culture 44 According to AABB Standards, at least 90% of all Apheresis Platelets units tested shall contain a 'Giy minimum of how many platelets? a 55x10 b 65«10 © 3.010 d 5.010 45. According to AABB Standards, Platelets prepared from Whole Blood shall have at least Gey a 5.5 x 10! platelets per unit in at least 90% of the units tested b 65x 11" platelets per unit in 90% of the units tested € 7.5 x 10! platelets per unit in 100% of the units tested 85 x 10! platelets per unit in 95% of the units tested 46 Which of the following is proper procedure for preparation of Platelets from Whole Blood? 4 light spin followed by a hard spin 'b light spin followed by 2 hard spins € 2iight spins 4. hard spin followed by a light spin 6 The Board of Certification Study Guide1: Blood Bank | Blood Products Questions 47 According to AABB standards, what is the minimum pH required for Platelets at the end of the Ii, storage period? 260 b 62 © 68 470 48 According to AABB standards, Platelets must be: a gently agitated if stored at room temperature b separated within 12 hours of Whole Blood collection € suspended in sufficient plasma to maintain a pH of 5.0 or lower prepared only from Whole Blood units that have been stored at 4°C for 6 hours 49° Aunit of Whole Blood-derived (random donor) Platelets should contain at least: 421.010" platelets b 55510" platelets € 5.5» 10) platelets 4 90% of the platelets from the original unit of Whole Blood 50 Platelets prepared by apheresis should contain at least: 2110! platelets b 3x10" platelets € 3x10" platelets @ 5x10" platelets $1 Leukocyte Reduced Red Blood Cells are ordered for a newly diagnosed bone marrow candidate NS, What isthe best way to prepare this produet? ‘8 crossmatch only CMV-seronegative units D irradiate the unit with 1,500 rads € wash the unit with saline prior to infusion 4. transfuse through a Log! leukacyte-removing filter 52. Of the following blood components, which one should be used to prevent HLA alloimmunization Uy of the recipient? ‘a Red Blood Cells b Granulocytes Irradiated Red Blood Cells _ Leukocyte- Reduced Red Blood Cells 53. A father donating Platelets for his son is connected to a continuous flow machine, which uses the principle of centrifugation to separate Platelets from Whole Blood. As the Platelets are harvested, all other remaining elements are returned to the donor, This method of Platelet collection is known as: 2 apheresis b autologous € homologous 4 fractionation 54 To qualify as a donor for autologous transfusion a patient's hemoglobin should beat least B g/dl 80 g/t) 11 wal 10 g/L) 13 g/dL (30 g/1) 15 g/aL 50 g/L) ane (Clinical Laboratory Certification Exerinations 71: Blood Bank | Blood Group Systems Questions ‘a ABO and Rh typing only | b ABO/Rh type, antibody screen 55. What is/are the minimum pretransfusion testing requirement(s) for autologous donations collected and transfused by the same facility? © ABO/Rh type, antibody screen, crossmatch 4 no pretransfusion testing is required for autologous donations 58. Inaqualty assurance program, Cryopreciptated AHF most contain a minimum of how many ‘hy international units of Factor VIII? a 60 b 70 © 80 430 MGiy units (U) of Factor VIII per ml. of Cryoprecipitated AHE Ifthe volume is 9 mL, whatis the Factor | 57 _Anassay of plasma from a bag of Cryoprecipitated AHF yields a concentration of 9 international VILL content of the bag in 1U? ao b 18 627 481 Blood Group Systems 58 Refer to the following table: Antigens / 1 23 45 | z! + oo es { Mo o + oe Qmo +s eo | Fwo ++ oe Ve +e 00 auto ‘Test results Given the most probable genotypes of the parents, which of the following statements best describes the most probable Rh genotypes of the 4 children? a 2are Ryr, 2are RR b Bare Ryr, Lisrr € Lis Ryr, Lis Ryr, 2are RyRy @ Lis Ror’ Lis RyRy, 2are Ryr 59 The linked HLA genes on each chromosome constitute a(n): a allele i b trait € phenotype | 4 haplotype 1B The Board of Certification Stady Guide1: Blood Bank | Blood Group Systems Questions 60 61 62 63 65 66 o7 ‘An individual's red blood cells give the following reactions with Rh antisera: fent-D enti-C anti anti-c antie Rh control 4 0 Bo ‘The individual's most probable genotype is: 8 DCe/DeE b Dek/dee « Deeldce 4 DCe/ace ‘blood donor has the genotype: hh, AB. What is his red blood cell phenotype? A aoe B ° a AB An individual has been sensitized to the k antigen and has produced anti-k, What is her most probable Kell system genotype? a kK b kk < kk 4 Kok Given the following typing results, what is this donor's racial ethnicity? Leb Fya-be: lard) 2 African American Asian American Native American @ Caucasian ‘A mother has the red cell phenotype D+CsB=c-e+ with anti-e (titer of 32 at AHG) in her serum, “The father has the phenotype D+C+E~c+e+. The baby is Rh-negative and not affected with hemolytic disease of the newborn, What is the baby's most probable Rh genotype? ary br «RR a Rr Jn an emergency situation, Rh-negative red cells are transfused into an Rh-positive person of the ‘genotype CDe/CDe. The first antibody most likely to develop is a antic b anti-d © antie 4 anti-E Most blood group systems are inherited as 2 sex-linked dominant b sex-linked recessive € autosomal recessive autosomal codominant ‘The mating of an Xg(a+) man and an Xg(a-) woman will only produce: Xg(a-) sons and Xg(a-) daughters B Xglas) sons and Xglar) daughters © ea-) sons and Xg(a+) daughters 4 Xg(a+) sons and Xgla-) daughters (Clinical Laboratory Certifcetion Exeminations 91: Blood Bank | Blood Group Systems Questions 68 Refer tothe following data ant ant anteE anti-c anti-e Given the reactions above, which is the most probable genotype? a Ry B Ry" © Ror" 4 Rik 69 A patient’s red cells type as follows: anti-D antic anti-€ 4 a ° Which of the following genotype would be consistent with these results? 2 RoR b Ry © RyRy a Ry 70 The red cels of a nonsecretor (se/se) will most likely type as: a Le(a-b) Bb Le(arb) € Lelasb-) 4 Le(a-bs) 71 Which of the following phenotypes will react with anti-f? an BRR, © RR, @ RR, 72 Apatient’s red blood cells gave the following reactions: anti antic anti-€ ante. = anticg—_anti-t + + + * + ° ‘The most probable genotype of this patient is RiRy b Ry” © Ry a RR, 73. Anti-N is identified ina patient's serum. If random crossmatches are performed on 10 donor SME, units, how many would be expected to be compatible? ° 7 10 74 Awoman types as Rh-positive, She has an antic titer of 32 at AHG. Her baby has a negative DAT and isnot affected by hemolytic disease of the newborn, What is che father’s most likely Rh phenotype? ber © Rr a Ry anes 10 The Board of Certification Study Guide1: Blood Bank | Blood Group Systems Questions 75 Which of the following red cel typings are most commonly found in the African American donor population? = Lua) B JkG@-b-) © Fy(a-b-) ak 76 Four units of blood are needed for elective surgery. The patient's serum contains anti-C, anti-e, anti-Fy* and anti-Jk®, Which of the following would be the best source of donor blood? atest all units in current stock B test 100 group O, Rh-negative donors € test 100 group-compatible donors 4 rare donor file 77 A donor is tested with Rh antisera with the following results: eot-D —ant-C—antE antic antic Rh control + + ° + + ° What is his most probable Rh genotype? a RR b Rr © Ror a Ry 78 family has been typed for HLA because 1 of the children needs a stem cell donor. Typing results ae listed below: fatner 3:88.35 mother: A220:812.38 chies —at2.08.2 hig #2 a123:08.10 cohlé #3) A3.25:818.7 What isthe expected B antigen in child #3? a AL BAD © B12 4 B35 72. Which ofthe following is the best source of HLA-compatible platelets? oH mother b father € siblings cousins ‘80 Apatient is group O, Rh-negative with anti-D and anti-K in her serum. What percentage of the MSiy_ general Caucasian donor population would be compatible with this patient? 205 b 20 © 3.0 460 (Clinical Laboratory Certification Examinations 121: Blood Bank | Blood Group Systems Questions 81 The observed phenotypes in a particular population are: Str Phenotype Number of persons Ionb=) 2 kasen 194 ee-bs) as ‘What is the gene frequency of Jk? in this population? 2031 b 045 © 058 4080 82 _ Ina random population, 16% of the people are Rh-negative (rr). What percentage of the My Rh-positive population is heterozygous for 7? 36% b 49% © 57% 4 66% 83 In elationship testing, a “direct exclusion’ is established when a genetic marker isi 44 absent in the child, but present in the mother and alleged father Bb absent in the child, present in the mother and absent inthe alleged father present in the child, absent inthe mother and present in the alleged father 4. present in the child, But absent in the mother and alleged father 84 Relationship testing produces the following red cell phenotyping results: ABO Rn ‘ataged father, 8 DeC-crEr0- ‘mother ° DsGrE-c-o8 chile: ° DsCre-cras What conclusions may be made? 4 there is no exclusion of paternity Bb paternity may be excluded on the basis of ABO typing paternity may be excluded on the basis of Rh typing 4 paternity may be excluded on the basis of both ABO and Rh typing 85 In a relationship testing case, the child has a genetic marker that is absent in the mother and ‘cannot be demonstrated in che alleged father. What type of paternity exclusion is this known as? 2 indicect Db direct € prior probability Hardy-Weinberg 86 A patient is typed with the following results: Pationt’s cols with Patient's serum with ana 0 Acradcalls 26 ant8 0 Brodcols 4+ antha® 2+ Ab screen 0 ‘The most probable reason for these findings is that the patient is group: ‘4 0; confusion due to faulty group O antiserum b O; with an anti-A; € Aywith an anti-A @ Ay;with an anti-a 12 The Board of Certification Study Guide1: Blood Bank | Blood Group Systems Questions ‘87 Human blood groups were discovered around 1900 by Jules Bordet b Louis Pasteur ¢ Karl Landsteiner 4d PLMollison 8B Cells of the As subgroup will a react with Dolichosbiflorus b bE-with anti-a, © givea mixed-field reaction with anti-A,B @DE- with anti 89. The enzyme responsible for conferring H activity on the red cell membrane is alpha ‘& galactosyl transferase 1b N-acetylgalactosaminyl transferase L-fucosyi transferase N-acetyiglucosaminyl transferase an 90 Even in the absence of prior transfusion or pregnancy, individuals withthe Bombay phenotype (©,) will always have naturally occurring ‘91 The antibody in the Lutheran system that is best detected at lower temperatures is, 4 anticLu’ b anti-Lu © anti-Lu3 4 anticLui? 92 Which of che following antibodies is neutralizable by pooled human plasma? SY a antickn® D anti-ch € antiVie! @ antics 83. Anti-Sd¥is strongly suspected if 4 the patient has been previously transfused b the agglutinates are mixed-field and refractile © the patient is group A or B only a small number of panel cells are reactive 96 HLA antibodies are: a naturally occurring b induced by multiple transfusions © directed against granulocyte antigens only 4 frequently cause hemolytic transfusion reactions 95. Genes of the major histocompatibility complex (MHC): code for HLA-A, HLA-B, and HLA-C antigens only b are linked to genes in the ABO system € are the primary genetic sex-determinants 4 contribute to the coordination of cellular and humoral immunity. (Clinical Laboratory Certification Examinations: 131: Blood Bank | Blood Group Systems Questions 96 _Isoimmunization to platelet antigen HPA-Ta and the placental transfer of maternal antibodies ER, would be expected to cause newborn erythroblastosis, B leukocytosis « leukopenia thrombocytopenia 97 Saliva from which of the following individuals would neutralize an auto anti-H in the serum of a group A, Le(a-b+) patient? 4 group A, Le(a-b-) B group A, Le(arb-) © group O, Letasb-) 4 group O, Le(a-bs) 98 Inhibition testing can be used to confirm antibody specificity for which of the following antibodies? a antiLut b antic € anti-Le’ a antify 99 Which of the following Rh antigens has the highest frequency in Caucasians? aD bE ec de 100 Anti-D and anti-C are identified in the serum of a transfused pregnant woman, gravida 2, para ‘ity. 1. Nine months previously she received Rh immune globulin (RhIG) after delivery. Tests of the patient, her husband, and the child revealed the following: ‘anti-D antl-¢ anti-e anti-c patient 0 o ° fatnor + ° ° ‘his + o 0 The most likely explanation for the presence of anti-C is that this antibody is: actually anti-C¥ b from the RhIG dose © actually anti-G naturally occurring, 101 The phenomenon of an Rh-positive person whose serum contains anti-D is best explained by: MY a gene deletion missing antigen epitopes ¢ trans position effect @ gene inhibition 102. When the red ces of an individual fail to react with anti-U, they usually fail to ceact with: a antiM 14 the Board of Certification Study Guide1: Blood Bank | Blood Group Systems Questions 108 Which ofthe following red cell antigens are found on glyeophorin-A? a MN b Le, Leb «Ss 4 PP, Pe 104 Paroxysmal cold hemoglobinuria (PCH) is associated with antibody specificity toward which of the following? ‘a Kell system antigens Duffy system antigens € Pantigen 4 I antigen 105. Which of the following is a characteristic of anti? ‘2 associated with warm autoimmune hemolytic anemia 'b found in the serum of patients with infectious mononucleosis € detected at lower temperatures in the serum of normal individuals found only in the serum of group O individuals 106 Ina case of cold autoimmune hemolytic anemia, the patient's serum would most likely react 4+ at immediate spin with: group A cells, B cells and O cells, but not his own cells b cord cells but not his own or other adult cells € all cells of a group O cell panel and his own cells d only penicilin-treated pane! cells, not his own cells 107 Cold agglutinin syndrome is associated with an antibody specificity toward which of the following? a Fy bP el @ Rha 108 Which of the following is a characteristic of anti-? ‘& often associated with hemolytic disease of the newborn b reacts best at room temperature or &°C © reacts best at 37°C is usually igG 109 The Kell (K1) antigen is: ‘absent from the red celle of neonates b strongly immunogenic © destroyed by enzymes has a frequency of 50% in the random population 120 In chronic granulomatous disease (CGD), granulocyte function is impaired. An association exists {Six between this clinical condition and a depression of which of the following antigens? a Rh be © Kell Duffy Ctinicol Laboratory Certification Examinations 151: Blood Bank | Blood Group Systems Questions ua a2 113, 4 11s 1s u7 ‘The antibodies of the Kidd blood group system: ‘4 react best by the indirect antiglobulin test bare predominantly IgM often cause allergic transfusion reactions 4 do not generally react with antigen-positive, enzyme-treated RBCs Proteolytic enayme treatment of red cells usually destroys which antigen? a Je bE ck ak Anti-Fy is usually acold-reactive agglutinin 1b -more reactive when tested with enzyme-treated red blood cells € capable of causing hemolytic transfusion reactions 4 often an autoagglutinin Resistance to malaria is best associated with which of the following blood groups? a Rh bli ©? 4 Dutty ‘What percent of group O donors would be compatible with a serum sample that contained anti-X. and anti-Y if X antigen is present on red cells of 5 of 20 donors, and Y antigen is present on red cells of 1 of 10 donors? a 25 b 68 « 250 d 680 How many Caucasians in a population of 100,000 will have the following combination of phenotypes? ‘System Pnenotype Frequency (%) ABO. ° 46 Gm Fo 48 Pow a4 37 50 a 18 aol b 14 «144 4 1,438 What is the approximate probability of finding compatible blood among random Rh-positive units for a patient who has anti-c and anti-K? (Consider that 20% of Rh-positive donors lack cand 30% lack K) 21% & 10% a 18% 45% 16 The Board of Certification Study Guide1: Blood Bank | Physiology and Pathophysiology Questions 118 A 25-year-old Caucasian woman, gravida 3, para 2, required 2 units of Red Blood Cells. ‘The antibody screen was positive and the results of the antibody panel are shown below: —M Col D Cc £ © K Jkt JkD Let Le? MON P, 37°C ANG to 440 0+ + + + 0 4 4 ee 0 OO 2 4+ +0 040+ 0 0 + +00 0 0 B+ 0+ 4 00 + + 0 + Fee DO 4 + ee 0+ 00 + Oe FO OD 5 00+ 0+0+ + 0 + +00 0 4 6 00+ + + O + 0 + 0 + + oO 0 tH TO 00+ 0+ + + + + O Hee 0 6 00+ 0+00 + 0 + 0+ + 0 auto 00 ERI onfancorent medio ‘What is the most probable genotype of this patient? bre © Ror a RR, Physiology and Pathophysiology 129 A man suffering from gastrointestinal hleeding has received 20 units of Red Blood Cells in the last {'ii, 26 hours and is stil oozing post-operative. The following results were obtained. Pr: 20 seconds (control: 12 seconds) APT: 43 seconds (control: 31 seconds) Platelet count: 160 x 109/pL (160 x 10%) gp: 10 gic (100 9/1) Factor i 6556 What blood product should he administered? 8 Fresh Frozen Plasma Red Blood Celis, ¢ Factor Vill Concentrate 4 Platelets 220 Transfusion of which of the following is needed to help correct hypofibrinogenemia due to DIC? 7 a Whole Blood b Fresh Frozen Plasma © Cryoprecipitated AHF 4 Platelets 121 A blood component used in the treatment of hemophilia Ais. a Factor VII Concentrate b Fresh Frozen Plasma © Platelets: @ Whole Blood (tinicel Laboratory Certification Exeminations 171: Blood Bank | Physiology and Pathophysiology Questions 122Which of the following blood components is most appropriate to transfuse to an S-year-old male fiiy,-Remophiliac who is about to undergo minor surgery? ‘2 Cryoprecipitated AHF b Red Blood Cells € Platelets Factor Vili Concentrate 123 Aunt of Fresh Frozen Plasma was inadvertently thawed and then immediately refrigerated at °C 2s on Monday morning, On Tuesday evening this unit may still be transfused as a replacement for 4 all coagulation factors b Factor V Factor VIII Factor x na 124 A newborn demonstrates petechiae, ecchymosis and mucosal bleeding. The preferred blood Mil. component for this infant would be: Red Blood Cells bb Fresh Frozen Plasma € Platelets 4 Cryoprecipitated AHE 325 Which of the following would be the best source of Platelets for transfusion inthe case of ‘ir alloimmune neonatal thrombocytopenia? a father »b mother € pooled platelet-rich plasma 4 polycythemic donor 126 An obstetrical patient has had 3 previous pregnancies. Her first baby was healthy, the second was jaundiced at birth and required an exchange transfusion, while che third was stillborn, Which of the following is the most likely cause? ‘4 ABO incompatibility b immune deficiency disease € congenital spherocytic anemia 4 Rhincompatibility 127. Aspecimen of cord blood is submitted to the transfusion service for routine testing. The following. My results are obtained: anti: anti-B: antl: Rrcontrot direct antiglabulin test a negative a6 negative 2 It is known that the father is group B, with the genotype of ede/ede, Of the following 4 antibodies, which 1 is the most likely cause of the positive direct antiglobulin test? 2 antia & anti-D © antic a antic 128 ABO-hemolytic disease of the newborn: usually requires an exchange transfusion 1b most often occurs in first born childen € frequently cesults in stillbirth 4 is usually seen only in the newborn of group O mothers 1B The Board of Certiication Study Guide1: Blood Bank | Physiology and Pathophysiology Questions 129 Which of the following antigens is most likely to be involved in hemolytic disease of| the newborn? ale bP, eM 4 Kell 130 ABO hemolytic disease of the fetus and newborn (HDFN) differs from Rh HDFN in that 1a Rh HDEN is clinically more severe than ABO HDFN b the direct antiglobubn testis weaker in Rh HDFN than ABO Rh HDFN occurs in the first pregnancy 4 the mother's antibody screen is positive in ABO HDN 131 The following results were obtained “ anti-A anti-B anti-D WeakD DAT Ab screen infant 0 wt ww ce ° nt an. reat renter Which of the following is the most probable explanation for these results? 8 ABO hemolytic disease of the fetus and newborn b Ri hemolytic disease of the fetus and newborn; infant has received intrauterine transfusions € Rh hemolytic disease of the fetus and newborn. infant has a false-negative Rh typing 4 large fetomaternal hemorrhage 182 A group A, Rh-positive infant of a group O, Rh-positive mother has a weakly positive direct antiglobulin test and a moderately elevated bilirubin 12 hours after birth. The most likely cause is: ‘2 ABO incompatibility b Rhincompatibility € blood group incompatibility due to an antibody to a low frequency antigen neonatal jaundice met associated with blood group 133. In suspected cases of hemolytic disease of the newborn, what significant information can be Shin obtained from the baby’s blood smear? ‘4 estimation of WBC, RBC, and platelet counts marked increase in immature neutrophils (shift to the left) € a differential to estimate the absolute number of lymphocytes present 4 determination of the presence of spherocytes 134. The Liley method of predicting the severity of hemolytic disease of the newborn is based on the amniotic fui ‘2 bilirubin concentration by standard methods Bb change in optical density measured at 450 nm Rh determination 4 ratio of lecithin to sphingomyelin 135. ‘These laboratory results were obtained on maternal and cord blood samples: mother: Re baby: ABs, DAT: 3+ cord hemoglobin: 10 g/d. (190 9) Does the baby have HDN? ‘& no, as indicated by the cord hemoglobin byes, although the cord hemoglobin is normal, che DAT indicates HDN € yes, the DAT and cord hemoglobin level both support HDN @ no, a diagnosis of DN cannot be established without cord bilirubin levels (Clinical Laboratory Cercifcation Examinations: 19)1: Blood Bank | Physiology and Pathophysiology Questions 138 The main purpose of performing antibody titers on serum from prenatal immunized women is Co: a determine the identity of the antibody b identify candidates for amniocentesis or percutaneous umbilical blood sampling decide ifthe baby needs an intrauterine transfusion determine if early induction of labor is indicated, 137. Which unit should be selected for exchange transfusion ifthe newborn is group A, Rh-positive and the mother is group A, Rh-positive with anti-c? a A,CDe/CDe Bb [Link]/cDE «© O,cde/ede d Avcdelede 138 4 mothers group A, with anti-D in her serum, What would be the preferred blood product if an hey intrauterine transfusion is indicated? ‘2 0, Rh-negative Red Blood Cells 'b O, Rh-negative Red Blood Cells. Irradiated © A, Rh-negative Red Blood Cells 4. A, Rh-negative Red Blood Cells, Irradiated 139 Laboratory studies of maternal and cord blood yield the following results: Shy Maternal bleed Cord blood. [Link] 8, Rn-positive anti€inserum OAT = 2+ antic€ in eluate If exchange transfusion is necessary, the best choice of blood is: a B Rh-negative, Bs b B,Rh-positive, B+ € O,Rh-negative, E~ 4 0, Rh-positive, E- 140 A blood specimen from a pregnant wornan is found to be group B, Rh-negative and the serum contains anti-D with a titer of 512, What would be the most appropriate type of blood to have available for a possible exchange transfusion for her infant? 20, Rh-negative b O,Rh-positive ¢_B, Rh-negative 4B, Rh-positive 141. Blood selected for exchange transfusion must: ‘a lack red blood cell antigens corresponding to maternal antibodies b be <3 days old € be the zame Rh type as the baby be ABO compatible with the father 142. When the main objective of an exchange transfusion isto remove the infant's antibody-sensitized ted blood cells and to control hyperbilirubinemia, the blood product of choice is ABO compatible Fresh Whole Blood Red Blood Cells (RBC) washed RBC suspended in Fresh Frozen Plasma heparinized Red Blood Cells nace 20 The Board of Certification Study Guide1: Blood Bank | Physiology and Pathophysiology Questions 143 To prevent graft-vs-host disease, Red Blood Cells prepared for infants who have received intrauterine transfusions should be: a saline-washed b irradiated € frozen and deglycerolized 4 group- and Rh-compatible with the mother 144 Which of the following isthe preferred specimen for the intial compatibility testing in exchange MM, transfusion therapy? 42 maternal serum b eluate prepared from infant’: red blood celle € paternal serum @ infant's postexchange serum 145. Rh-lmmune Globulin is requested for an Rh-negative mother who has the following results > Deonrol Weak Weak D controt mother’s postpartum sample: 0 ° 0 ° i= anode What is the most likely explanation? a mother is a genetic weak D 1b mother had a fetomaternal hemorrhage of Ds cells | © mother’s red cells are coated weakly with IgG anti-D reagent is contaminated with an atypical antibody 146 The following results are seen on a maternal postpartum sample: { D Dcontrot Wes Weak D control i mother's postpartum sempie: 0 0 1 ° ini= mee tara ‘The most appropriate course of action is to: ‘a report the mother as Rh-negative b report the mother as Rh-positive © perform an elution on mother’s RBCs investigate for a fetomaternal hemorthage 147. What is the most appropriate interpretation for the laboratory data given below when an Rh-negative woman has an Rh-positive child? Rosette fetal screen using enzyme-treated D+ cells, mother's sample: 1 rosstte/3 ‘otos positve control: Srosettes/S fields egative contra! no rasettes observed mother is not a candidate for Rhlg mother needs ] vial of Rhlg mother needs 2 vials of Rhlg the fetal-maternal hemorrhage needs to be quantitated anes (Clinical Laboratory Certification Examinations: 221: Blood Bank | Physiology and Pathophysiology Questions 148) 149 150 151 182 153 154 Refer to the following information: Postpartum anti-O Rhcontrol_WeakD Weak D control Rosette fetal screen. motner ° ° + micro a 20 rosettes fees awoom rs ° nt ONT ra ATeroresed What is the best interpretation for the laboratory data given above? mother is Rb-positive mother is weak D+ ‘mother bas had a fetal-maternal hemorrhage mother has a positive DAT A weakly veactive anti-D is detected in a postpartum specimen from an Rh-negative woman, During her prenatal period, ail antibody screening tests were negative, These findings indicate: a that she is a candidate for Rh immune globulin, b that she is not a candidate for Rh immune globulin € anced for further investigation to determine candidacy for Rh immune globulin 4. the presence of Rh-positive cells in her circulation ‘The results of a Kleihauer-Betke stain indicate a fetomaternal hemorrhage of 35 mL. of whole blood. How many vials of Rh immune globulin would be required? 1 fetomaternal hemorchage of 35 mL of fetal Rh-positive packed RBCs has been detected in an th-negative woman. How many vials of Rh immune globulin should be given? ° b a A RI 1 2 a3 Criteria determining Rh immune globulin eligibility include: a mother is Rh-positive b infane is Rh-negative mother has not been previously immunized to the D antigen 4 infant has a positive direct antiglobulin test While performing routine postpartum testing for an Rh immune globulin (RIG) candidate, a weakly positive antibody screening test was found, Anti-D was identified. This antibody is most likely the result of ‘a massive fetomaternal hemorrhage occurring at the time of this delivery 'b antenatal administration of Rh immune globulin at 28 weeks gestation contamination of the blood sample wich Wharton jelly _ mother having a positive direct antiglobulin test Rh immune globulin administration would not be indicated in an Rh-negative woman who has atn): 2 first trimester abortion 'b husband who is Rh-positive © anti-D titer of 1:4,095, 4 positive direct antiglobulin test 22 Tee Board of Certifcation Study Guide ee1: Blood Bank | Physiology and Pathophysiology Questions 155 A Kleihauer Betke stain of a postpartum blood film revealed 0.3% fetal cells. What is the estimated volume (mL) of the fetomaternal hemorrhage expressed as whole blood? a5 b 15 <3 a 35 156 Based upon Kleihauer-Betke test results, which of the following formulas is used to determine the volume of fecomaternal hemorrhage expressed in mL. of whole blood? of fetal cells present » 30 % of fetal cells present 50 4% of maternal ells present « 30 % of maternal cells present « 50 nage 157 An acid elution stain was made using a L-hour post-delivery maternal blood sample. Out of 2,000 calls that were counted, 30 of them appeared to contain fetal hemoglobin, Its the policy of the medical center to add 1 vial of Rh immune globulin to the calculated dose when the estimated volume of the hemorrhage exceeds 20 mL of whole blood. Calculate the number of vials of Rh immune globulin that would be indicated under these circumstances. 2 3 4 5 anor 158 The roserte test will detect a fetomaternal hemorrhage (FMH) as small as: 2 10mb b1Smb © 20mb @ 30m 159.10 mi fetal maternal hemorrhage in an Rh-negative woman who delivered an Rh-positive baby ‘iy means that the: mother's antibody screen willbe positive for anti-D rosette test willbe postive mother is nota candidate for Rh immune globulin mother should receive 2 dases of Rh immune globulin anon 160 Mixed leukocyte culture (MLC) isa biological assay for detecting which of the following? a HLA-A antigens b HLA-B antigens, ¢ HLA-Dantigens 4 immunoglobulins 161. A.40-year-old man with autoimmune hemolytic anemia due to anti-E has a hemoglobin level of, 10.8 g/AL (108 g/L) This patient will most likely be treated with: 8 Whole Blood b Red Blood Cells ‘¢ Fresh Frozen Plasma no transfusion 162 A patient in the immediate post bone marrow transplant period has a hematocrit of 21%. The red cell product of choice for this patient would be: packed saline washed ‘microaggregate filtered irradiated nace (Clinica Laboratory Certification Exeminations: 231: Blood Bank | Serology Questions 163 HLA antigen typing i important in screening for. 2 ABO incompatibility b akidney donor € Rhincompatibility 4 ablocd donor 164 DR antigens in the HLA system are Sg. significant in organ transplantation Bb not detectable in the lymphocytotoxicity test «¢ expressed on platelets expressed on granulocytes 165 Anti-E is identified ina panel at the antiglobutin phase. When check cells are added to the tubes, ro agglutination is seen. The most appropriate course of action would be to: 44 quality control the AHG reagent and check cells and repeat the panel B open a new vial of check cells for subsequent testing that day € open a new vial of AHG for subsequent testing that day 4 record the check cell reactions and report the antibody panel result Serology 166. A serological centrifuge isrecalibrated for ABO testing after major repais, “Time in seconds 18 202580 's button delineates? yes yes yes yos. ln supernatant clear? moyen yee you button easy toresuspend? yes «yes ye 0 strength of racton? am tee Given the data above, the centrifuge time for this machine should be: ‘a 15 seconds b 20seconds © 2S seconds d 30 seconds 167 Which of the following represents an acceptably identified patient for sample collection and transfusion? ‘a ahandwritten band with patient's name and hospital identification number is affixed to the patient's leg b the addressographed hospital band is taped to the patient's bed ¢ an unbanded patient responds positively when his name is called the chart transported with the patient contains his armband not yet attached 24 The Board of Certification Study Guide1: Blood Bank | Serology Questions 168) 169 170 im 172 173 ‘Samples from the same patient were received on 2 consecutive days. ‘Test results are summarized below: Day m1 Day #2 anti-a a ° anti-B ° a anti-D 3+ 3 Aycels ° a Beols 4 ° ‘Ab screen ° ° How should the request for crossmatch be handled? 1 crossmatch A, Rh-positive units with sample from day 2 crossmatch B, Rh-positive units with sample from day 2 € crossmatch AB, Rh-positive units with both samples 4 collect a new sample and repeat the tests ‘The following test results are noted for a unit of blood labeled group A, Rh-negative ted with: fani:B antD ° a What should be done next? a transfuse as a group A, Rh-negative B transfuse as a group A, Rh-positive ‘¢ notify the collecting facility discard the unit What information is essential on patient blood sample labels drawn for compatibility testing? ‘4 biohazard sticker for AIDS patients patient's room number € unique patient medica! number 4 phlebotomist initials Granulocytes for transfusion thould: 44 be administered through a microaggregate filter 1} be ABO compatible with the recipients serum ¢ be infused within 72 hours of collection 4 never be transfused to patients with a history of febrile transfusion reactions A neonate will be transfused for the first time with group O Red Blood Cells. Which of the following is appropriate compatibility testing? «2 crossmatch with mother’s serum crossmatch with baby’s serum € no crosemateh is necessary if inital plasma screening is negative 4 no screening oF erossmatching is necessary (or neonates A group B, Rh-negative patient has a positive DAT. Which of the following situations would occur? a all major crossmatches would be incompatible Bb the weak D test and control would be positive € the antibody screening test would be positive the forward and reverse ABO groupings would not agree (Clinical Laboratery Certification Examinations: 251: Blood Bank | Serology Questions 274 The following reactions were obtained: Cols tested with: Sorum teated with: anti-A anti-B ant-AB Aces Bells ae ae 2 a ‘The technologist washed the patient's cells with saline, and repeated the forward typing, A saline replacement technique was used with the reverse typing, The following results were obtained: Calls tested with: ‘Serum tested with antiA anti-B antAB — Aycalls Boe 4&0 te 0 a ‘The results are consistent with: acquired immunodeficiency disease b Bruton agammaylobulinemia multiple myeloma acquired "8" antigen an 175. What is the most likely cause ofthe following ABO discrepancy? Patient's cells vs: Pationt’s serum v3: ani-A antes Avcols Bealls oO ° ° 2 recent transfusion with group O blood b antigen depression due to leukemia € false-negative cell typing due to rouleaux 4 obtained from a heel stick of a -month old baby 176 Which ofthe following patient data best reflects the discrepancy seen when a person's red cells demonstrate the acquited-B phenotype? Forward grouping Reverse grouping patinta 8 ° patient AB, A ! patintc 0 8 patient 8 AB aa bE eC aD 177 Which of the following is characteristic of Ta polyagglutinable red cells? 12 if group O, they may appear to have acquired a group A antigen Bb they show strong reactions when the cells are enzyme-treated € they react with Arachis hypogaea lectin the polyagglutination is a transient condition 1178. Mixed field agglutination encountered in ABO grouping with ne history of transfusion would most likely be due to: 4 Bombay phenotype (O;) B Tactivation € Agredcells 4 positive indirect antiglobulin test 179 Which of the following is a characteristic of polyagglutinable red cells? can be classified by reactivity with Ulex europaeus are agglutinated by most adult sera are always an acquired condition autoconteot is always positive aaoe 26 The Board of Certifcation Study Guldeood Bank | Serology Questions 180 Consider the following ABO typing results: Patient's cells vs atient’s serum vs: ant ont-8 As cells 0 * Additional testing was performed using patient serum 1s RT screening cet 2 screening cat 62 fautocontrl = ts ‘What isthe most likely cause of this discrepancy? 8 Apwith anti-A; cold alloantibody cold autoantibody 4 acquired-A phenomenon Consider the following ABO typing results: Pationt’s calls ve: Patient's serum ve: ant ante Aycels Beels & 0 “ 4 Additional testing was performed using patient serum: is ORT seraening cal! 142 screening call i+ 2a autocontol = i+ De What should be done next? 18 test serum against a panel of group O cells b neutralization € perform serum type at 37°C elution ‘The following results were obtained on a patient’s blood sample during routine ABO and Rh testing: Call testing: Serum testing: ania 0 Acoli: 4s aniB as Boel 2 anid: 0 autocontrot 0 Select the course of action to resolve this problem, ‘= draw a new blood sample from the patient and repeat all test procedures Bb test the patients serum with Ay cells and the patient's red cells with anti-A, lectin € repeat the ABO antigen grouping using 3x washed saline-suspended cells 4. perform antibody sereening procedure at immediate spin using group O cells Which of the following explains an ABO discrepancy caused by problems with the patient's red blood cells? an unexpected antibody rouleaux agammagiobulinemia Tn activation Clinical Laboratory Cesifcation Esrninations 271: Blood Bank | Serology Questions 184 The test for weak D is performed by incubating patients red cell with: several different dilutions of anti-D serum anti-D serum followed by washing and antiglobulin serum anti-D® serum 4 antiglobulin serum 185: Refer tothe following data: tity Forward group: Reverse group: ant-R at anti-A lectin Avcalls Apcols Bealls 4&0 te o a ae Which ofthe following antibody screen results would you expect with the ABO discrepancy seen above? a negative bb positive with all screen cells at the 37°C phase positive with all screen cells at the RT phase; autocontral is negative 4 positive with al screen cell and che autocontrol cells atthe RT phase 186 ‘The following results were obtained when testing a sample from a 20-year-old, first-time blood donor: Forward group: Reverse group: fant antieB Avcelis Bells ° ° ° 3s What is the most likely cause of this ABO discrepancy? loss of antigen due to disease B acquired B € phenotype 0), “Bombay” weak subgroup of A 187 A mother is Rh-negative and the father Rh-positive. Their baby is Rh-neyative. It may be concluded that: the father is homozygous for D the mother is heterozygous for the father is heteronygous for D at least 1 of the 3 Rh typings must be incorrect aece 188 Some blood group antibodies characteristically hemolyze appropriate ced cells in the presence of ‘4 complement b anticoagulants € preservatives 4 penicillin 189. Review the following schematic diagram: PATIENT SERUM + REAGENT GROUP “O" CELLS INCUBATE — READ FOR AGGLUTINATION WASH — ADD AHG -> AGGLUTINATION OBSERVED ‘The next step would be to: add “check cells” asa confirmatory measure Bb identify the cause of the agglutination ¢ perform an elution technique 4. perform a direct antiglobulin test 28 the Board of Certification Stuy Guide1: Blood Bank | Serology Questions 190 The following results were obtained in pretransfusion testing: i arc vat screening ceil! 0 ae ° 3+ autocontol 0 a ‘The most probable cause of these results is & rouleaux b awarm autoantibody € acold autoantibody multiple alloantibodies 4391 A patient is typed as group 0, Rh-positive and crossmatched with 6 units of blood. At the indirect, antiglobulin (IAT) phase of testing, both antibody screening cells and 2 crossmatched units are incompatible, What is the most likely cause of the incompatibility? 4 recipient alloantibody b recipient autoantibody «€ donors have positive DATS 4 rouleaux 192 Refer to the following data hemoglobin: 74 gid (74 g/t) retioviocyte count: 22% Direct Antiglobulin Test ‘Ab Screen -1AT polyspeciic: 3+ Sci 8 IgG 36 Sir 34 o. ° auto: 3+ ‘Which clinical condition is consistent with the lab results shown above? ‘a cold hemagglutinin disease 'b warm autoimmune hemolytic anemia ¢ penicillin-induceé hemolytic anemia delayed hemolytic transfusion reaction 193 A patient received 2 units of Red Blood Cells and had a delayed transfusion reaction Pretransfusion antibody screening records indicate no agglutination except after the addition of IgG sensitized cells. Repeat testing of the pretransfusion specimen detected an antibody at the antiglobulin phase. What is the most likely explanation for the original results? red cells were overwashed b centrifugation time was prolonged € patient's serum as omitted from the original testing 4. antighobulin reagent was neutralized 194 At the indirect antiglobulin phase of testing, there is no agglutination between patient serum and screening cells. One of 3 donor units was incompatible ‘The most probable explanation for these findings is that the: 1» patient has an antibody directed against a high incidence antigen ‘b patient has an antibody directed against a low incidence antigen ¢ donor has an antibody directed against donor cells donor has a positive antibody screen 195 The major crossmatch will detect a(n) ‘8 group A patient mistyped as group O Bb unexpected red cell antibody in the donor unit ‘¢. Rh-negative donor unit mislabeled as Rh-positive recipient antibody ditected against antigens on the donor red cells (Clinical Laboratory Certification Bxeminations 291: Blood Bank | Serology Questions 196 A 42-year-old female is undergoing surgery tomorrow and her physician requests that 4 units of Red Blood Cells be crossmatched. The following results were obtained. 1s sre ar screening calli 0 ° ° screening call ° o seraening calill 0 ° ° Grossmatch 1S, src war donert: B+ 1% * donors 23.4 0 0 ° What is the most likely cause of the incompatibility of donor 1? ‘a single alloantibody b multiple alloantibodies © Rh incompatibilities donor 1 has 2 positive DAT 197 Which of the following would most likely be responsible for an incompatible ntiglobulin crossmatch? ‘a recipient’ red cells possess alow frequency antigen anti antibody in donor serum € recipient's red cells are polyagglutinable 4 donor red cells have a positive direct antiglobutin test 1198 A reason why a patient's crossmatch may be incompatible while the antibody sereen is negative is: fa the patient has an antibody against a high-incidence antigen B the incompatible donor unit has a positive direct antiglobulin test cold agglutinins are interfering in the crossmatch the patient's serum contains warm autoantibody 199 Ablood specimen types as A, Rh-positive with a negative antibody screen. 6 units of group A, Rh-positive Red Blood Cells were crossmatched and 1 unit was incompatible in the antiglobulin phase, The same result was obtained when the test was repeated. Which should be done first? b a 200 During emergency situations when there is no time to determine ABO group and Rh. repeat the ABO grouping on the incompatible unit using a more sensitive technique test a panel of ced cells that possesses low-incidence antigens perform a direct antiglobulin test on the donor unit obtain a new specimen and repeat the crossmatch type on a current sample for transfusion, the patient is known to be A, Rh-negative. The technologist should. efuse to release any blood until the patient's sample has been typed release A Rh-negative Red Blood Cells release O Rh-negative Red Blood Cells release O Rh-positive Red Blood Cells 201 A.29-year-old male is hemorrhaging severely. He is AB, Rh-negative. 6 units of blood are required STAT Of the following types available in the blood bank, which would be most preferable for crossmatch? nage 2 AB Rh-positive b A, Rh-negative € A,Rh-positive 40, Rh-negative 30 The Board of Certification Study Guide1: Blood Bank | Serology Questions 302 A patient ic group AB, Rh-positive and has an antighobulin. reacting anti-A; in his serum. He is in the operating room bleeding profusely and group Az® Red Blood Cells are not available, Which of the following blood types is fst choice for crossmatching? 2B Rh-positive BB Rhonegative A,B, Rh-positive 40, Rhenegative 203 A 10% red cell suspension in saline is used in a compatibility test. Which of the following would most likely occur? 1a afalse-positive result due to antigen excess b false-positive result due to the prozone phenomenon € a false-negative result due to the prozone phenomenon 4. false-negative result due to antigen excess 204 A patient serum reacts with 2 of the 3 antibody screening cells at the AHG phase. 8 of the 10 iS ynte crosematched were incompatible at the AHG phase. All reactions are markedly enhanced by enzymes. These results are most consistent with antic B anticé anti-c @ ant-Fy* 205 A patient received 4 units of blood 2 years previously and now has multiple antibodies. He has not been transfused since that time, It would be most helpful to ‘a. phenotype his cells to determine which additional alloantibodies may be produced B recommend the use of directed donors, which are more likely to be compatible ‘€ use proteolytic enzymes to destray the “in vitro” activity of some of the antibodies ! @ freeze the patient's serum to use for antigen typing of compatible units 206. Autoantibodies demonstrating blood group specificity in warm autoimmune hemolytic anemia are associated more often with which blood group system? a Bh bi cP d Duty 207 An antibody that causes in vitro hemolysis and reacts with the red cells of 3 out of ten, crossmatched donor units is most likely: a anti-Let b anti € antick 4 anti 208 A patient's serum reacted weakly positive (14%) with 16 of 16 group O panel cells at the AHG MS, test phase The autocontrol was negative. Tests with ficin-treated panel cells demonstrated no reactivity at the ANG phase, Which antibody is most likely responsible for these results? anti-ch anti-k ante antiJs> anor | Clinical Laboratory Certification Bxaminations: 311 1: Blood Bank | Serology Questions 209. An antibody identification study is performed with the 5-cell panel shown below: Antigens: 12 3 4 5 Testresuits wi + 00 +e fe gu 9 o+o+ 0 m oo ++ +o 0 & Sw oo +e oe e Vio+ ++ 00 + auto [An antibody against which of the following antigens could mot be excluded? al b2 3 a4 2410 A.25-yearold Caucasian woman, gravida 3, para 2, required 2 units of Red Blood Cells “The antibody screen was positive and the results of the antibody panel are shown below: —M Col BD Coc Eo K Jkt JkY bof Le? MON P, 37°C AHG 1 4 + 0 0 + + ee Oe ee ° ° 2 + + 0 0 + Oo + oO + + ° ° ee ee ) w ee) + 0 + + O + 0 . 5 0 0 + 0 + oO + 0 + 0 0 0 * 6 0 0 + + e Oe . s+ 0 0 1 7 00 0 4 0 + 4+ He ee Oe ee 1 8 0 9 + Oo + © Oe oO + 0 ew 1% auto 0 ° Which of the following antibodies may be the cause of the positive antibody screen? a anti-M and anti-K b anti-c and anti © anti-Jk? and anti-c @ anti-P; and anti-e 32. The Board of Certification Study Gulde1: Blood Bank | Serology Questions 211_A25-yearold Caucasian woman, gravida 3, para 2, requited 2 units of Red Blood Cells. MS, The antibody screen was positive and the results of the antibody panel are shown below: —M coh DB Cc E © K Jk Jk Let Leb MON P, 97°C AKG 14 + 0 Oe ee He Oe He He OO 2 + + 0 © o + 0 0 + + 0D oO oO oO a 4 oO 4s oe He Oe HH HO te 4 4 4 4 0 6 0 0 + Oe + OH Oe 5 0 0 + O + 6 + + O + + 0 0 oO 6 0 0 + + + Oo + oO + DO + + OO T 0 0 + Oe ee ee Oe HO 8 0 0 + oO + oO 0 + 0 + OH Oe ato 0 wr aent media Which common antibody has met been ruled out by the panel? b anti-Le? antiJkt 4 anti 212 In the process of identifying an antibody, the technologist observed 2+ reactions with 3 of the 10 cells in a panel after the immediate spin phase. There was no reactivity after incubation at 37°C ‘and after the anti-human globulin test phase. The antibody most likely is: a anti; B anti-Let © anti @ anti-Fy? 213 Transfusion of Che (Chido-positive) red cells toa patient with anti-Ch has been reported to cause: ‘8 no clinically significant red cell destruction B clinically significant immune red cell destruction € decreased “ICr red cell survivals 4 febrile transfusion reactions 214 Results of a serum sample tested against a panel of reagent red cells gives presumptive evidence Sly. of analloantibody directed against a high incidence antigen. Further investigation to confirm the specificity should include which of the following? ‘8 serum testing against red cells from random donors b serum testing against red cells known to lack high incidence antigens € serum testing against enzyme-treated autologous red cells, testing of an eluate prepared from the patient's red cells (Clinical Laboratory Certification Examinations: 33: Blood Bank | Serology Questions 215 Refer to the following data: Forward group: Reverse group: até anteB anti-A, lectin Aycelis Apcols Balls 0 a ° a a ‘The ABO discrepancy seen above is most likely due to: anti-A) b rouleaux © anti-# unexpected IgG antibody present 216 Refer to the following panel: EM coh 0 Cc E& © K Jkt Jk Let Lee MON P, 37°C ANG CD 2 2 + + 0 oe Oe o + + 0 0 Oo 3 Bo + 0 + 4 0 Oo + + Oe HHH He ee) ° 5 0 0 + 0 + O + + O + + O ° 2 6 0 0 + + + oO + oO + Oe oe 7 00 + Oe + s4 0 + ee OO 2 8 0 0 + 0 + 0 oO + oO + Oo + + ° auto 0 ° ERT arharcamient media Based on the results of the above panel, the most likely antibodies are: a antioM and anti-K bb anci-8, anti-Jk' and anti-K © anti-Ji and anti-M anti-B and anti-Le? 217 Which characteristics are true of all 3 of the following antibodies: anti-Fy*, anti-Jieé, and anti-K? ‘a. detected at [AT phase and may cause hemolytic disease of the fetus and newborn (HDEN) and transfusion reactions bb not detected with enzyme treated cells; may cause delayed transfusion reactions © requires the [AT technique for detection; usually not responsible for causing HEN, 4 may show dosage effect; may cause severe hemolytic transfusion reactions 34 Tae Board of Certification Study Guide1: Blood Bank | Serology Questions 218 Refer to the following cell panel: = Enzymes Cel D C c E e@ K Jk® Jk Le* Le? MN P, AHG AHG Poe oo eee pO ed eee me 2 ss oer oe re re 3 + 0 + + OO + 0 + 4+ # # O 0 4044 40+ 00 + oO + + Oe mw BS cos os a+ +0 + +00 0 0 6 Co ++ + oy 0 + 0 ++ 0 0 T 00+ 0+ ee we Oo eee OD OO ®@ 00+ 0+ 00+ 04+ 0+ + 0 OF mio oo Based on these results, which of the following antibodies is most likely present? a antic b anti © anti-D @ antik 219 4 pregnant woman has a positive antibody screen and the panel results are given below: m —M Enzyme Cell D Gc E © K Jk JkD Fy? Fy Let Leb MNP, 37°C AHG AHG Toss 00+++ + 0 + Oo + ee 0 0 Boss 00+0% 0 + 0 oe voo He mw Oo i ee a ne) 4 ee 40+00 + 0 + 0 + 04 0 0 0 5 00+0+0+ + + + 0 oo 0 wo 6 0044404 0 0 0 + o +0 0 ° T 00+0++ 4 + Oe + 0 +e 0 o @ oo+0+00 + + 0 oe se ow mo atoo 00 EXI= rhancoront media What is the association of the antibody(ies) with hemolytic disease of the newborn (HDN)? ‘2 usually fatal HDEN 1b may cause HDFN € isnot associated with HDEN 4 HDEN cannot be determined 220 Which of the following tests is most commonly used to detect antibodies attached to patient's ted blood cells in vivo? anes direct antiglobulin complement fixation indivect antiglobulin immunofluorescence (Clinical Laboratory Certification Bxasinations: 35221 223 224 225 226 227 228 Blood Bank | Serology Questions Anti-l may cause a positive direct antiglobulin test (DAT) because of: anti- agglutinating the cells b C3d bound to the red cells © Tactivation 4 C3c remaining on the red cells after cleavage of C3b Which direct antiglobulin test results are associated with an anamnestic antibody response in a recently transfused patient? Tost result ——Polyspeciic.§— Ig 3 Controt rasult 8 “ ° ° result 8 a 0 ° result © a a ° ° result 0 a a te ° iianed Tes a result A b result B € result C result D In the direct (DAT) and indirect (IAT) antiglobulin tests, false-negative reactions may result ifthe: {4 patient's blood specimen was contaminated with bacteria Bb patient's blood specimen was collected into tubes containing silicon gel € saline used for washing the serurv/cell mixture has been stored in glass or metal containers 4 addition of AHG is delayed for 40 minutes or more after washing the serum/cell mixture Polyspecific reagents used in the direct antiglobulin test should have specificity for: ses specificity IgG and Iga B IgG and C34 © IgMand Iga gM and C34 In the direct antiglobulin test, the antiglobulin reagent is used to: ‘2 mediate hemolysis of indicator red blood cells by providing complement 'b precipitate anti-erythrocyte antibodies © measure antibodies in a test serum by fixing complement detect preexisting antibodies on erythrocytes AHG (Coombs) control cells: 4 can be used as a positive control for anti-C3 reagents b canbe used only for the indirect antiglobuiin test € ave coated only with IgG antibody d_ must be used to confirm all positive antiglobulin reactions AS6-year-old female with cold agglutinin disease has a positive direct antiglobulin test (DAT). When the DAT is repeated using monospecific antiglabulin sera, which of the following is most likely tobe detected? a Ig b lec © C34 4 Cha ‘The mechanism that best explains hemolytic anemia due to penicillin is: 1 drug-dependent antibodies reacting with drug-treated calls 1b drug-dependent antibodies reacting in the presence of drug © drug-independent with autoantibody production 4 nonimmunologic protsin adsorption with positive DAT 36 The Board of Cartiscation Study Guide