UNIVERSITY OF THE PUNJAB... Roll No.

First Semester 2018

Examination: B.S. 4 Years Programme

PAPER: Botany-I (Plant Diversity) TIME ALLOWED: 30 mins.'..

Course Code: BOT-101 MAX. MARKS: 10
Attempt lhis Paper on this Question Sheet only,
PART I _ OBJECTIVE (10 MARKS)
Q. No. l: Multiple Choice euestions: Tick the Correct answer.
\
l. ------------- species ar.e comnrorrly l<nolvn as blue or green molds.
(a). Penic'illium (b). Llucor
(c). Rhizopus
(d). Puccinia
2. ------------ is used by prokaryotic cells tbr locomotion.
(a). Pilli
(b). Mitochondria
(c). Flagella (d). Endospore
3 blur.tileu is heterosporous a.d sori are produced in rrLriting
bodies cailed
(a).synangia (b). sporocarp
O. stobili (d). None of these
4. lrr smur fungi, the site ol karyogamy and rneiosis is ----------

(a). basidiospoe (b). teliospore

(c), parasitic mvceliunr (d). none olthese
5. Pinnularia pant is shaped like an elongate box ensheathed in
a siliceous rvall often called
a --------------
(a). valve (b). frustule
(c). silica (d). none ofthese
6. Synangiunr is the characteristic feature of......
(a). Selaginella (b). Psilotum
(c). Equiserum (d). Marsilea
7' The Ectocarpus species are golden brown in coror due to presence
of dominant
(a). lucoerythrin (b). lucoxanthin
(c). beta-carotene (d).bothb&c
8. In ---------- -----, the life cycle is triphasic.
(a). Ectocarpus (b). Polysiphonia
(c). Churo (d). none ofthese
9. ------------ are commorrly called arnphibious plants.
(a). Bryophytes (b). Pteridophytes
(c). Cyrnnosperrns (d). none olthese
10. A firlly lormed infectious viral parlicle is termed as --____--____--
(a). viroid (b). virusoid
(c ). v iron (d). caosid
 UNTVERSITY OF THE PUNJAB.. t., Roll No.
First Semester 2017
Examination: B.S. 4 Years Prosramme
(Special Examination) \

PAPER: Botany-I (Plant Diversity) TIME ALLOWED: 30 mrns. r.

Course Code: BOT-101/11300 MAX. MARKS: 10
Attempt this Paper on this Question Sheet only,
PART I - OBJECTIVE (10 MARKS)
Q.1 Multiple choice questions: Tick the correct answers. (10 Marks)

i. Viral genome attached to the bacterial genome is termed as:

a) Bacteriophage b) Prophage c) Lysogeny d) Lysis

ii. The transmission of a double stranded piece of DNA from a donor bacterium to
recipient through a tube is known as:

a) Transformation b) Sex pilli c) Binary Fission d) Conjugation

iii. Which structure is used by Prokaryotic cells for locomotion?

a) Pilli b) Mitochondria c) Flagella d) Endospore

iv. Reproductive organs are produced in the form ofcones in:

a) Angiosperms b) Gymnosperms c) Conifers d) Cryptogams

v. Sori are produced in hard bodies in Mursilea known as:

a) Basidiocarps b) Seeds c) Synangia d) Sporocarps

vi. The type of fruiting body formed in Phyllactinia is:,

a) Perithecium b) Apothecium c) Basidiocarp d) Cleistothecium

vii. Aeciospores are carried to wheat leaves where they germinate to form:

a) Telia b) Basidia c) Uredinia d) Pycnia

viii. The protostele in which xylem core is Smooth and rounded is:

a) Plectostele b) Haplostele c) Actinostele d) Polycyclic

ix. Pteridophytes are also called:

a) Phanerogams b) Vascular Cryptogams c) Spermatophytes d) Gymnosperms

x. The dwarf shoots of pine tree with its foliage leaves is called:

a) Spur b) Needle c) Strobilus d) Indusium

 I]NIVERSITY OF THE PUNJAB
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First Semester 2017
Examination: B.S. 4 Years Programme i RottNo......................a at:
(Special Examination) 'ooo r r r a a..aaaaaaaa. at o

PAPER: Botany-I (Plant Diversity) TIME ALLOWED:2 hrs. & 30 mins.

Course Code: BOT-101/11300 MAX. MARKS: 50
Attempt lhis Paper on Separate Answer Sheet provided,

PART II SUBJECTIVE

Q. 2. Answer the following questions briefly. (20 MARKS)

a. Give symptoms of TOBACCO MOSAIC DISEASE.

b. Explain the role of PILLI in bacteria.
c. Differentiate between TINILOCULAR and PLURILOCULAR SPORANGIA.
d. Describe briefly the structure of GLOBULE of Chara.
e. Differentiate between PROTOSTELE and SIPHONOSTELE.
f. Explain the internal structure of Physcia.
g. Differentiate between ASCOMYCETES and BASIDIOMYCETES.
h. How cell wall of bacteria differ from plant cell wall?
i. Write dilferences between Pteridophytes and Gymnosperms.
j. Describe the structure ofarchegoniumin Funaria.

Q. 3. Answer the following questions comprehensively. All questions carry equal marks.

(30 MARKS)

i. (a) Give an account on general characteristics of BRYOPHYTES. (5 Marks)

(b) Describe the SEXUAL REPRODUCTION in bacteria. (5 Marks)

It. (a) Compare the characteristics of CHLOROPHYTA and RHODOPHYTA (5 Marks)

(b) Explain the life cycle of RUST FUNGI with illustration. (5 Marks)

llt. (a) Describe the ultrastructure of a typical virus. Illushate with an example. (5 Marks)
(b) Explain internal structure of the stem of Ephedra. Also draw its
Neat and labeled diagram. (5 Marks)
 T]NIVERSITY OF THE PUNJAB
Second Semester - 2018 . Roll No.

\\
PAPER: Botany-Il \
(Plant Systematic Anatomy & Development Theory) TIME ALLOWED: 15 Mints. '...
MAX. MARKS: 10 '.
Course Code: BOT-103 / BOT-12300 Part - I (Computsory)

Attemot this Paoer on this uestion Sheet onlv.

Please encircle the correct option. Esch MCO carries 1 Marll This Paper will be collected
back after exoirv of time limit mentioned above.

Q t. Multiple Choice Questions (1x10=10)

i. Arlangcnient ol: leal'tirr stern is cirllcti

a) Cryptogamy b) t'hytlo<le c) Phyllotaxy d) rrone o1'l-hem

2. Olclest avaitable litcra.ture in botanv is

a) Species plantrrrurn b) lJistoria ptantarurr c) Philosophia botanica d) none ol'tlrenr

3, Ca;tsianr.fi"tritescens is the scientillc name o1'

a) Bltrck peppeL b) [-ove applc c ) lted pepper cl) Shinrla nrircl.r

4.[-)rrgrlrorLriaceae Iarrily lrave spccial type o{ inllorescence called

a) Racenre b) CyathiLrm c) Capituiunr d) Cytnose

5. ,\ctccharlmoJficinttntnt is the inost rlnpttltanl source oi'...... .. irr the world

a) CaLbohyclrates b) I)rc,rcins c) Sugat' o) t.ipids

6. Pelicycle is the characteristic lbatLrre o1'

a) Sterri b) lioot c) I-,eat.cs ii )I:'Lor'vct

'7. ['arcrrohyrua celIs lravirig cirololopIasts cronstllLlto

e) Ch lor.opirl,lltrLts parencitl'trr ir ) .ColIertol.rf inir c1 ehlor:enchl rua d) /-\eretrchytlrrr

8. Cotl< carnbiunr is arr cxarllrle of
a) Apical rneristern b) lntercalatl,nrerislenr c) Prirnaly nreristeur d) [.,ateral rneristem

9. Stignrrr is the rrodi:lcation o1'the tissue cIat tl.re apcx of tlre ..

a) ()var'y br Style c) L.arpcl d,1 A rtlrer'

l0. \\rhioh ol'the follttrvirrg Iiving r:clIs give tl're nrechantctrl support ancl strength to plant?

a) Sclerenchyma b) Phloem c) Stone cells d) Collenchyma

 UNI\IERSITY OF TI{E PUNJAB a aaaa aa aaaaat
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Second Semester - 2018 .a

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PAPER: Botany-Il
(Plant Systematic Anatomy & Development Theoty) TIME ALLOWED: 2 Ers. & 45 Mints.
Course Code: BOT-103 / BOT-12300 Part - II MA)L MARKS:50

Attempt this Paper on Separate Answer Sheet provided.

(10x2=20)
Short Qtrt:stious (each questiotr t'.arr)' {rvo tratks)

I . Cortrpare natural ancl phylogeneLic s)/slelns ol classi[lcation

2. Detlne cr-yptogam)' atrcl p['rauerogaln5'

-1. Enlist lLrles of, trotrrellclztlure

4. D flercn iate ltclrvecr t l)li lill)' ;) 3 rrt tcl i':1lot,l'pe

i t

5. Write lbtrr clistingirisltinq li.lrttrle of i:rrll lr,,{stet aci-ae i

6. WriLc fotrr botanictrl tranrt:s ol plants belongirrg tc' the larnily' Solattaceae
7. DifttLentiate betweetl parerlchylrla ancl chlorenchynra
8. l)itferentiate betweett exarclr and errclerch xylern ntatttt'ation pattern
9. Compare Flypog,vnv and l:pigy'nv u'itlr cliarh'ams
10. Corrpar:e motlocot and clicot stern

l)ct:rilctl (]ucs t ir.r ns (i(')

(-i)
t. (a). Exp la: rr the nroderrl s)'sl"crl r o l' cl assiilcat iorr,
(:i
(Lr). LJrrloid tletits arrtl il'-'rrli'r il.s ''rl this ciiissillc;li()11 s\':ltetli )

(a). \\/rite a lrrief rrote on the ilrlat(]tllical Jeatrrres olcell rvall'

(j)
2.
(5)
(b). l)iscLrss the developrtrettt tll'tLatlsitiorl zoll(: area in any *'ood;' plarrt
(lr;. I)clirru Llt.'Ltttt.:, lllirl\)l)\ lj. .;-:iliv,,t:r;ll illl(l slll'':llll',lls
(r)
J.

ol' |'iace nLatiotr (7)

(tr). r\lso tlescribe dil'telt:rrL t1'pes
 UNTVERSITY OF THE PT]NJAB
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Third Semester 2018 i :

Examination: B.S. 4 Years Prosramme :..*lf .IJ::'.':'.':'j:'.';:':'i:'i'..:
PAPER: Botany-III (Cetl Biolory, Genetics and Evolution) TIME ALLOWED:2 hrs. & 30 mins.
Course Code: BOT-201/21300 MAX. MARKS: 50
Attempt thh Paper on Separate Answer Sheet provided
SUBIECTIW PART

Question 2: Attempt any l0 of the foltowing questions. Each question carries two marks.

1 .
How carbohydrates and sugars are used by the body?
2. How ribosomes are involved in protein synthesis?
3. What are nucleic acids?
4. Define evolution?
5. What are the types of plastids?
6. Differentiate between nucleus and nucleolus.
7. Describe the role of pachytene in meiosis.
8. What is the chemical composition of cell membrane?
9. What is lnversion mutation?
10. Differentiate between alleles ancl gene.
11. What is the Mendel law of independent assortment?
12. What is a genetic code?
13. What is back cross?
14, Defrne segregation
15. What is a stop codon?

Question 2: Attempt any two of the following three questions.

1: a) How vacuoles play an important role in a plants cell (07)

b) What is sex linked inheritance? Explain with examples? (08)

2: a) What are the glyoxysomes and peroxysomes and discuss their functions (08)

b) Discuss gene expression regulation (The Lac operon\. (07)

3: f)iscuss thc basic genetic Engineering techniques and its role in revolutionizing modern life (15)
 I]NIYERSITY OF THE PUNJAB'... Roll No.
Third Semester 2018

PAPER: Botany-III (Cell Biolory, Genetics and Evolution) TIME ALLOWED: 30 mini.'..
Course Code: BOT-201/21300 MAX. MARKS: 10

Attemptthis Paper on this Question Sheet only,

OBIECTIW PART
Question 1: Attempt all the questions, Circle the correct option, Each question carries one mark

t. The degradative Processess are categorized under the heading of

(A) Anabolism (B)Catabolism (C) Metabolism (D) None of the above
2. The rnost active site of protein synthesis is the

(A) Nucleus (B) Ribosorne (C) Mitochondrion (D) Cell sap

3. The ability of the cell membrane to act as a selective banier depends upon
(A) The lipid cornposition of'the membrane (B) 1'he pores which allows small molecules
(C) I'he special rnediatecl transporr systems (D) All of these

4. The Golgi complex

(A) Synthesizes proteins (B) Produces ATP (C) Provides a pathway for transporting

chemicals (D) Fonns glycoproteins

5 I'lrc sugar found in RNA is

(A) Ribose (B) Deoxyribose (C) Ribulose (D) Erythrose

6. The functions of plasma albumiu are
(A) Osmosis (B) Transport (C) Imrnunity (D) both (A )and (B
1. ln quaternary structure. subunits are linked by
(A) Peptide bonds (B) Disutphide bonds (C) Covalent bonds (D) Non-covalent bonds
8. A nucleoside consists of
(A) Nitlogenous base (B) Purine or pyrimidine base t sugar (C) Purine or pyrimidine base +

phosphoro us (D) Purine * pyrimidine base * sugar + phosphorous

().
In contrasl to eukaryotic mRNA , prokaryotic mRNA
(A) Can be polycistronic (B) Is synthesized with introns (C) Can only be monocistronic
(D) Has a poly A tail
10. The structure of IRNA appears like a

(A) Helix (B) Hair pin (C) Clover Ieaf (D) Coil
 UNTYERSITY OF' TITE PUNJAB
Fourth Semester - 2018 ..RolI No.
Examination: B.S. 4 Years programme

\
!efE! Botany-fV @Iant Physiolory and Ecotory)
Course Code: BOT-203 I BOTA2300 Part I (Comnut
TIME ALLOWED: 15 Mints.
- MAXMARXS: IO

uestion Sheet onlv.

back after exoi of tlme limit mentioned above.

Q.t I . Stomatal opening is stimulated by
(1xl0=10)

a. Blue light
b. Red light

c. Creen light
d. UV Iight
The unit of chemic,al potential is

a. J nrol r

b. J mol-2
c. J rnol r
d. None
3. Watsr depth in the sedge meadow stage of hydmsere is
a. 6-8 feet
b. 4-6 feet

c. I foot
d. l-2 inches
4. The water in the depth and penlanenrly. saturated zone is called
a. Hygroscopic water
b. Cheurically combined water
c. Ground water
d. Gravitational water
5. Which food chain does nol stan from plants?
a_ Grazing
b. Detritus food chain
c. Pond food charn
d. Terrestrial food chain
6. Electrochemical gradient across plasma mernbmes
of plants is generated by
a. H+-Al'Pase
b. H+-pyrophosphatase
c. Both a&h
d. None
7. Which of the following plant hormones is invohd
in fruit ripening?
a. Abscisic Acid (AAA)
b. clrokinins
c. Ethylene
d. Auxins
8. 'l'he state ofwater
that moves in all directions iscalled
a. Capillarl'water
b. Hl,groscopic water
c. Gravitational water
d. Cornbined water
9. Which of the following is considered a macrcnutrienr for
mosr plants?
a. Iron
b. Copper
c. Magnesium
d. Manganese
10. Several Calvin cycle enzymes are more active at pH

a. plt:4
b. pH- 7
c. pH--8
d. Other than these
 UNTYERSITY OF THE PT]NJAB
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Fourth Semester - 2018 aa
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3 RolI No. ...........

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PAPER: Botany-IV (Plant Physiology and Ecolory) TIME ALLOWED:2 Hrs. & 45 Mints.
Course Code: BOT-203 IBOT-22300 Part - Il MA)LMARKS:50

Attempt this Paper on Separate Answer Sheet provided.

Q2. Answer the following question with short answer' (10x2=20)

I. Differentiate between soil texture and soil structure.

II. Cive trvo rnaiu types of population growth.
III. Differentiate between food web and food chain.
IV. What is the role of ABA in seed dormancy?
V. Differentiate between absorption and action spectrum.
Vl. What is the difference between an essential and beneficial nutrient element?
VII. Define plrotolespirat iort.
' Vili, How is wind att important envirotrmeutal factor for plants?
IX. How does the Casparian strip affect water movement into the plant?
X. What is the autogenic chauge incurred during succession,

Q 3. Answer the following questions briefly. (6x5:30)

[. Draw a graphic representation of the N cycle in nature. Write the salient features of this cycle'
II. Give in detail the characteristics of plant communities.
III. Diagram the flow of electrons that occur in linear electron transport in photosynthesis

IV. Differentiate between carrier, channel and purnps'

water- saturatsd conditions'
V. Give an account of hydrophytes that enable them to survivc under
VI. Describe the role of auxin in gravitropism in plants'
 .....
UNIVERSITY OF THE PUNJAB Roll No.
Fifth Semester 2018
Examination: B.S. 4 Years Programme

PAPER: Higher Fungi TIME ALLOWED:30 mlns. \.

Course Code: BOT-301 MAX. MARKS: 10
Attempt this Paper on this Question Sheet only,
OBJECTIVE TYPE
PART-I

Q1. Each question has four possible answers. Choose the correct answer and encircle it.
1. Fungi whichreproduce only by asexual means, and produce conidia

a. Are unable to undergo mitosis b. Are members of the Deuteromycota c. Lack an anamorphic
phase d. Lack a telomorphic phase
2. Stink horn is the common name of

a. Phallus b. Agaricus c. Lycoperdon d. None ofthe above

3. The fruiting body of Penicillium is called

a. Cleistothecium b. Perithecium c. Apothecium d. Stroma
4. Is an exarnple of Rust fuog*.
a. Puccinia b. Amanita c. Erysiphe d.Uncinula

5. Gills are produced in

a. Ganoderma b. Russula c. Alternaria d. Hydnum
6. Srnut is caused by
a. Ustilago b. Puccinia c. Alternaria d. Cercosporo"
7, Dolipore septa are the characteristic feature of mycelium
a. Asomycetes b. Lichens c. Basidiomycetes d. Deuteromycotina
8. The thallus of lichen contains
a. Algal cells b. Fungal hYPhae c. a&b d) None of these
9. Plants get benefit from the fungus forming symbiotic association in the form of

a. Water b. Minerals b. Carbohydrates d.a&b

10. Region where basidiospores are attached to the basidium is termed as

a. Sterigmata b. Pileus c. Raphe d. None ofthe above

 UNIVERSITY OF THE PUNJAB aa aaa a a a aa a a a ara a a aa a " t'a

Fifth Semester 2018

Examination: B.S. 4 Years Programme i RouNo...................... i

PAPER: Higher Fungi TIME ALLOWED: 2 hrs. & 30 mins.

Course : BOT MAX. MARKS: 50

Attempt this Paper on Separate Answer Sheet provided.

PART-II
2. Briefly answer the following questions. Each question carries two marks. 20 Marks
L Differentiate between PEZIZALES and APHYLLOPHORALES ?

2. What is the difference between PARTIAL and TINIVERSAL VEIL?

3. What are UREDINIOSPORES and AECIOSPORES?
4. What are different types of CONIDIAL development in ascomycetes?
5. Give a diagrammatic representation of ASCUS development?
6. Differentiate between a PYCNIUM and AECIUM?
7. What is the economic importance of SACCIIAROMYCE9
8. What are OPERCULATE and IOPERCULATE DISCOMYCETES? GiVC SOME EXAMPICS.

9. What are HYPOGEOUS and EPIGEOUS fruiting bodies?

10. Differentiate between IGIRICUS and AMANITA with labeled diagrams.

SUBJECTM QUESTIONS 30 Marks

Q3. Answers the following questions.

1. Write a note on ERGOT OF RYE disease caused by CI.AVICEPS PURPUREA. Draw labeled
Iife cycle. (05 marks)
2. Give general characters of LICHENS. Also explain their anatomy with stiitable tabeled diagram.
(05 marks)

3. Write beneficial role of fungi in ecosystem. (05 marks)

4, What is black stem rust of wheat? Give methods to control this disease. (05 marks)
5. What is CENTRUM? Write a note on different types of Centrum found in ASCOMYCOTINA.
(10 marks)
 ....
UNIVERSITY OF THE PUNJAB Roll No.
X'ifth Semester 2018
Examination: B.S. 4 Years Programme
\\
PAPER: Microbial and Molecular Genetics TIME ALLOWED: 30 mins)'.
Course Code: BOT-303 MAX. MARKS: 10
Attempt this Paper on this Question Sheet only.

A. Choose the correct answer: 10

l. Plasmids are transferred between bacteria by way of a

i. transposon
ii. conjugator
iii. pilus
iv. snorkel
v. trichogyne

2. Which of these describes a Holliday junction?

i. A section of DNA where base pairing is not exact.

ii. A strand of DNA containing genetic material from two different chromosomes
iii. An interaction of two strands of DNA from homologous chromosomes
iv. A three shanded DNA structure where single stranded DNA has invaded a double helix.

3. Transcription is initiated when Rl[A polymerase binds to

i. promotor
a
ii. an initiator
iii. atranscriptor
iv. a codon

4. Which of the following genes is not a structural gene of the lac operon?

i. IacA
ii. lac I
iii. lac Y
iv.lac Z
5. This type of recomblnation does not require homologous sequences and is important for
the integration of viral genomes into bacterial chromosomes

i. replicative recombination
ii. general recombination
ii i. site-specific recombination
P.T.O.
iv. None of the above
 : ".'

6.WhichofthefollowinginvolvesdefectiveDNArepairmechanism?
i. HaemoPhilia
ii. Down SYndrome
iii. Xeroderma Pi gmentosum
iv. HYPertension

T.Thistypeofplasmidcanexistwithorwithoutbeingintegratedintothehost,s
chromosome

i. medisome
ii. lysosomes
iii. episome
iv. chromosome

8. Which of the following can be used as a tool by microbial geneticist?

i. Plasmids
ii. transPosable elements
iii. bacterioPhages
iv. All of the above

in
9. Stipped mispairing may cause deletions resulting

i. Insertional inactivation
ii. Translocations
iii.Single nucleotide substitutor
iv. Frame shift mutations
v. Error in nucleotide choice

10. Euchromatin is

i. Highly condensed
ii. Highly expressed
iii. Loosely packed
iv. None of the above
 UNIVERSITY OF THE PUNJAB
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Fifth Semester 2018 : :

Examination : B. S. 4 Years Prosramme i .*lr.oJ:: ;:...;...: ;;...:...: :.. j

PAPER: Microbial and Molecular Genetics TIME ALLOWED: 2 hrs. & 30 mins.
Course Code: BOT-303 MAX. MARKS: 50
Attempt this Paper on Separote Answer Sheet provided.
SUBJECTIVE

B. Explain the followingt 20

Q. Differentiate between

a. REPLICATIVE and CONSERVATM TRANSPOSITION

. b. SPONTANEOUS and INDUCED mutations
c. POLYMERASE and LIGASE
d. NUCLEOSOME andNUCLEOID Region
e. VIRULENT ANd TEMPERATE PHAGES
f. CISTRON and OPERON
g. LIGHT and DARK repair
h. INDUCTION and REPRESSION
i. COMPLEMENTATION and RECOMBINATION
J. BIOCHEMICAL MUTANTS ANd VISIBLE MUTANTS

C. Write brief answers: 30

l.What do you rurderstand by the term P-CYTOTYPE and M-CYTOTYPE? Write about the
mechansims involved in the transposition.

2. Explain the mechansims involved in mutations caused by BASE ANALOGUES with

reference to 5-Bromouracil and Aminopurine. Draw diagram where necessary.

3. What do you understand by the term "CATABOLITE REPRESSION"?

4. What do you mean by

*TRANSLOCATION"?

5. How can you map the genes using the process of CONJUGATION in prokaryotes?

6. Illustrate the structure of a BACTERIOPHAGE in detail. Draw labelled diagram also.

 UNIVERSITY OF THE PUNJAB Roll No.
Fifth Semester 2018
Examination: B.S. 4 Years Programme
\
PAPER: Evolutionary Trends in Trachaeophytes TIME ALLOWED: 30 mins)''..
Course Code: BOT-305 MAX. MARKS: 10 "
Attempt this Paper on this Question Sheet only.
OBJECTIVE TYPE
1. Multiple Coice Questions (10)
Encircle the correct options.
1) The Leaves which bear sperangia are called;
a. Sporophyll b. Bract c. Cone d. Strobilus
2) The process of double fertilization is unique in;
a. Gymnosperms b. Cycads c. Angiosperms d. Ferns
3) The best types of fossils are found as;
a. Compressions b. lmpressions c. lmprints d. Petrifactions
4) Evolution can be defined as;
a. Negative process b. Gradual change in characters c. Descend with change
d. Destructive change
5) Trachaeophytes are the plants with;
a. Large stems b. Epidermis c. Xylem and Phloem d. Seeds
6) Which of the following is not a Leptosporangiate Fern?
a. Dryopteris b. Adiantum c- Marattia d- Pteris
7) Earliest known Lycopsids are included in;
a. Drepanophycales b. Ophioglosales c. Gnetales
d. Ranales
8) Stems mostly equipped with ridges and forrows are characteristic for;
a. Ferns b. Dioon c. Sphenopsids d. Ables
9) Aglaophyton majoris a',

a. Trachaeophyte b. Gymnosperm c. Fern

d. Early Devonian Non-Vascular plant with hydroids and leptoids
10)Hymenophyllum is a;
a. Filmy Fern b. Primitive Algae c. Bryophyte d. Gymnosperm
 UNIVERSITY OF THE PUNJAB
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Fifth Semester 2018 aa
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Examination: B.S. 4 Years programme 3 Roil No. ..................... :
'orooo.ooaaa aa..or r ro....a

PAPER: Evolutionary Trends in Trachaeophytes TIME ALLOWED:2 hrs. & 30 mins.

Co ode: BOT-305 MAX. MARKS: 50

Attempt this Paper on a Separate Answer Sheet provided to you. Marks shall be deducted for
wrong spellings. Draw neat and labeled diagrams wherelrecessary.
SUtsJECTIVE TYPE
2. Give short and to the point answers. (2X10 = 20)

i. Differentiate between Bryophytes and Tracheophytes.

ii. Write down steps involved in the Evolution of Seeds.
iii. Differentiate between Leptosporangiate and Eusporangiate Ferns.
iv. Define the term "STELE" with examples.
v. What is MESXARCH XYLEM? Support your answer with neat and labeled diagram(s).
vi. Name any two AQUATIC FERNS.
vii. Enlist any four synthetic characters of ARTHROpHYTA.
viii. How FALSE INDUSIUM differs from TRUE INDUSIUM?
ix. What makes GNETALES most important ammg GYNMOSPERMS?
x. Highlight the status of FEMALE GAMETOPHyTE in ANGtospERMS.

3. Answer the following questions BRIEFLy. Maximum Marks = 30

Ql. Give a detailed account of the "GEOLOGICAL TIME SCALE' with reference to the
evolution of Vascular Plants. (i0 marks).
Q2. Write comprehensive note on the EVOLUTIONARY IMPORTNACE of ANGIOSPERMS
along with fHEORIES pertaining to their ORIGIN. (10 marks)
Q3. Compare and Contrast SPHENOPHYLLALES with EeUSETALES. Give examples along
with neat and labeled diagrams to support your answer. (10 marks)
 ....
UI\IVERSITY OF THE PUNJAB Roll No.
Fifth Semester 2018

\
PAPER: Environmental Biology TIME ALLOWED: 30 minsl'.
Course Code: BOT-307 MAX. MARKS: 10
Attempt this Paper on this Question Shbet only.
OBJECTIVE TYPE
10
Q 1. Encircle correct option of the statements given below'

(i) pH value of acid rain is less than

(a) 6.0 (b) s.7 (c) s.s (d) s.3

(ii) Organic wastes in water can

(a) Deplete dissolved oxygen of water (b) Cause cancer

(c) Cause excessive growth of algae (d) reduce photosynthesis

(iii) Pollutant gas in atmosphere involved in the production of ozone in the air is

(a) SOz (b) NH3 (c)NOz (d)cr

(iv) Ozone layer in atmospherg is located in

(a) Stratosphere (b) Troposphere (c) Exosphere (d) Mesosphere

(v) Higher the ICV (lnter-phase Chromosome Volume), higher is the radio-sensitivity in

(a) Higher plants (b) Lower plants (c) lnvertebrates (d) Higher animals

(vi) Major Contribution in global warming is that of

(a)cFC (b) Methane (c) coz (d) Nro

(vii) Unit used to measure Noise intensity is

(a) Joule (b) Decibel (c) Hertz {d) Kilometer

(viii) Radiations that can travel only up to a few centimeters in the air are known as

(a) X-Rays (b) Beta Particles (c) Gamma Rays (d) Alpha Particles

(ix) BOD is

(a) a measure of organic matter present in water (b) Biochemical OxYgen Demand
(c) usually less than COD (d) all a, b and c

(x) A species abundant in its natural range but with declining population is said to be

(a) ixtinct species (b) Threatened species (c) Endangered species (d) lndicator species
 UNIVERSITY OF THE PUNJAB
aaaaaaaataaa

Fifth Semester 2018

Examination: B.S. 4 Years Programrue ; RolI No' """"""""""'
....... :

PAPER: Environmental Biology TIME ALLOWED:2 hrs. & 30 mins.

: BOT- MAX. MARKS:50

Attempt this Paper on Separate Answer Sheet provided.

Q 2. Answer the following short questions (2.5 x 8)

(i) Differentiate between Troposphere and Stratosphere

(ii) What is Biological magnification?
(iii) The Fall out problem
(iv) Explain Ex situe conservation and its impacts
(v) Effects of Ozone on plant growth
(vi) Classify solid waste pollution
(vii) Greenhouse gases, their sources and impacts
(viii) Thermal pollution and its impacts

Give detailed answers of the following

Q 3. Enlist and discuss in detail general and specific adverse effects of water pollution 10

Q 4, Define photochemical smog. Write down the mechanism of its formation, diurnal variation 10
of its major pollutants and its impacts.

Q 5. What do you understand by greenhouse effect and global warming? Critically discuss nature
and role of greenhouse gases. What are impacts of global warming? 10
 I]NTYERSITY OF THE PUNJAB aa aaa a a a a a aa
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Sixth Semester - 2018 oa
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I Ro[No...................... I

PAPER: Plant Anatomy (Advance Course) TIME ALLOWED: 2 Hrs. & 45 Mints.
Course Code: BOT-311 Part - II MAX. MARKS:50

Attempt this Paper on Separate Answer Sheet provided.

Q. No.2: Give brief ansters of follou'ing qtlestions. (10x2=20)

l. What is siphonostele? \\rhat are its dilferent types?

2. Define Reaction wood. What are its different types'/

3. Di1't'erentiate l-lald wood ancl Soft wood in plants.
4. What are parench-tttra tissr-res? Cive therl' tirrlction.
5. DilfeLentiate betweeu vessel tnetnbers and tracheids.
6. Wh.v is the heart wood darker in color than sap wood?
7. Hou, is fascicLrlar canrbiurn dilferent fi'onr inter-tascictrlar cambjurn?
8. \\'hat is treani by 'CLrttation".'l.[o$' it is ]irrl<ed \'\'ith 'Hydatho'les'?

(10)
Q. No.3: Give a cletailed account on root-shoot transition'
(10)
Q. No. 4: Irxplain the lristology oI angiospernr leaf.
Q. No. 5: What is meant by Axial and Ray systems in Secondary
xylem? Explain in detail'

(10)
 T]NIVERSITY OF THE PUNJAB
Sixth Semester - 2018 '...Roll No. ..................

PAPER: Plant Anatomy (Advance Course) TIME ALLOWED: 15 Mints.

Course Code: BOT-311 Part - I MAX.MARKS: l0

Attempt this Paper ol this

Please encircle the correct ontion. Each MCO carries I Mark This Paoer will be collected
back sfter exDirv of time limit mentioned above.

Q. No. 1: Fill in the blanks rvith suitable tcrms. (lxl0=I0)

I . Preseltce of casparian srrips is a clraracteristics featuie of -----___

(a). Endoderrnis (b). Exoderm is

(c) Epiderrn is (C). Pericvclc

2. Vasculal burrdles in a dicot stenl are --------------,

(a). open. collateral. exarch (b) C losed. collatcral. ertrlailh
(c) CLosed. co llatc.r'a i. exarch (d). open. co Ilatera L errila:cll
-----*- is loltned on the lowel sides of leaning or clooliecl sients ol'
conif'er trees.

1a). Corrplession wood (b). 1'en sion wood

e . lleait rvood (d). F-arly rr,ood
:1. Trichotnes secreting the sticky substances are ----------------
(a). Clands (b). Colleters
(c). Stinging haiLs (d). I'>apillae
5. A leaf rn rvhich palisade parenchynra is plesent on Lroth abaxial and adaxial leal sLrrlhces

i: ',llrctJ ;rs --.- -

(a). Bitacial leaf (b). lsobilatelal leal

e. l)orsiventral (d). Foliage
5. Which of the follorving givc r.ise to corl( tissue?
(a). l'}hellogen (b). Periblern
O. Pelid ernr t d). P heilocier rn

7. CLLttation is a process clraraclelistic l,r --------.-.

('a). \ e.]taries (b) I-aricit ers

(c). Hydarhodes (d). Res in d ucts
The cbil iL1,' oi' rvoocl .o \ iiirstdnC Louing is called ------------
(a). p liability (b).'l'cxtLrre
(c). strengrh (d). DLrrability
ln *--------------------- 1.ype ol xylem, the developrnent of the xyler.r tal<es piace tou,rrtl
the centre of the axis,
(a). endarch (b). exalch
(c). nr esalch (d). Uoth a & t,
10. ln .-- type ol siphonostele, xy lent is sr.rllouncled orr loth
sides [ry rincs olphloenr.

(a). Anrphiphloic so lcnosteie (b). Ectophloic solenostelc

(c).botha&l; (d). none of these
 UNTVERSITY OF THE PTINJAB
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Sixth Semester - 2018 aa
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Examination: B.S. 4 Years Prosramme
!toooooooooooaaaaa
nonNo...................... :
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PAPER: Gene Cloning (Advance Course) TIME ALLOWED: 2 Hrs. & 45 Mints.
Course Code: BOT-313 Part- tr MAX. MARXS:50
Attempt this Paper on Separate Answer Sheet provided.

(2x10=20)
Q2: Answer the following:
What are ADAPTORS?
What do you mean by KLENOW tiagment?
What is the basic principle of GEL IILECTROPHORESIS?
Explain plasmid compatibility.
Give significance ofcloning 6-endotoxin in crop plants.
vi. Define TRANSFECTION.
vii. What do you mean by the term IN'IRONS?
viii. What is GENE TIIERAPY and for what purpose it is used?
ix. Explain briefly the process of SOUTFIERN BLOTTING.
x. What do you understand by the term Microinjection?

Q3: Explain the follorving: (6x5=30)

1. Whatdo youknow about COSMIDS?
2. IJxplain INSERTIONAL INACTIVATION of /ac Z gene.
3. Write down the procedure for the preparation of TOTAL CELL DNA.
4. What are the different classes of DNA manipulative ENZYMES?
5. Explain bricfly about phage M13 as CLONING vector.
6. Write about the method for the preparation of COMPETENT E. coli cells.
 UNIVERSITY OF THE PT]NJAB
Sixth Semester - 2018 . RollNo.

\
PAPER: Gene Cloning (Advance Course) TIME ALLOWED: 15 Mints.
Course Code: BOT-313 Part - I (Compulsory) MAX. MARKS: 10

Attemnt this Paoer on this uestion Sheet onlv.

Please encircle the corrgct option. Each MCO carries 1 Mark This Paoer v!'ill be collected
back after exoirv of time limit mentioned above.

Q1: Choose the correct answer: (1x10=10)

1. The second step in most genetic engineering

experiments is

a. Screening
b. Production of recombinant DNA
c. Cleavage of DNA
d. Cloning
e. Testing
",
Which of the following statement is true?
a. A vector should have an origin ofreplication
b. A vector should have selectable marters
c. A vector should have unique restriction site
d. All of these
J. In the.screening process, clones that metabolize
X_gal turn
a. Yellow
b. Orange
c. Red
d. Blue
e. White
4' Bacteria protect themserves from viruses by
fragmenting virar DNA upon entry with
a. Ligases
b. Methylases
c. Endonucleases
d. Vectors
e. Probes

5. Ior what purpose is DNA electrophoresis used?

1'o amplify a DNA for cloning
1.
b. To separate noncharged rnoleiules of Dn-A based on size
To separate charged molecules of DNA based on
:
d' size
fo separate charged moiecures of DNA based on amount of positive charge
e. To produce static electricity by friction

(P.r.o.)
6. Gene therapy targets
a. Genotypes
b. Phenotypes
c. Either a or b depending on the application
d. Both genotypes and phenotypes
7. In preliminary screening of clones, it is common to use
a. Restriction enzymes
b. Probes
c. Antibiotics
d. Dyes
e. Milipore filters

8. Ti plasmid which is uscd as plant vector is obtained from

a. Agrobacterium tumefaciens
b. Agrobacterium radiobacter
c. Thermus aquaticus
d. None ofthe above

9, Certain endonucleases cut DNA and leave DNA termini without overhangs which
are called

a. cohesive termini
b. sticky ends
c. blunt ends
d. oligonucieotides
e. none ofthe above

10._ is a filamentous phage

a. T2
b. l"
c. M13
d. T4
 UNIVERSITY OF' TIIE PUNJAB
Sixth Semester - 2018 aaoaa aaaaaa
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!ooolooo
RouNo. ..................... :
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PAPER: Plant Tissue Culture (Advance Course) TIME ALLOWED:2IIrs. & 45 Mints.
Course Code: BOT-315 Part - II MAX. MARKS: 50
Attempt this Paper on Separate Answer Sheet provided.

PART-II
Q2. QUESTIONS WITH SHORT ANSWERS (10x2-20 Marks)
1. Enlist the basic apparatus required in prant'r'issue culture Laboratory.
2. what is Sterilization? why is it so irnportant in a plant Tissue culture lab?
3. What are Callus Cultures?
4. What is meant by 'Medium undeflned'?
5. Enlist the rnajor components used in plant'l'issue culture *edia.
6' what are sonle of the praclical applications of plant Tissue culture?
7. what are the tw'o most significant applications of plant protoplast cultures.
8. How would you prepare virus-free plants using tissue culture rneans?
9. How can we prepare hybrids using plantTissue Culture techniques?
10. What is meant by the term 'CellularTotipotency'?

Q3. QUESTTONS WITH BRrEF ANSWERS (10x3-30 Marks)

I. what is meant by Aseptic Techniques? Give rnethods of Aseptic Technique in
plant tissue culture.
2. Write a brief account of Organogenesis in plant tissue culture.
3. Write note on
A) Cell suspension cultures.
B) Anther and poller-r culture.
 UNIVERSITY OF THE PUNJAB
Sixth Semester - 2018 . Roll No.

PAPER: Plant Tissue Culture (Advance Course) TIME ALLOWED: 15 Mints. ".
Course Code: BOT-315 part - I (Compulsor'i MAX. MARKS: 10 ".-
Attempt this Paner on this uestion Sheet onlv.

PART-I
Each question has four possible answers. choose the correct (or the most
appropriate) answer by encircling it' Each question carries equal marks.

Multirrle choice q uestions

(10)
Ql. Encircle the correct oPtion
I. Callus is usually sub-culturcd after
a) 1-2 weeks b) 2-3 weeks c) 4-6 rveeks d) varies as Per

experirnental requirement

2. Dry heat is used for sterilization of

a) (ilassr.vare b) I-iquid rrredia c) Hormonal solutions d) vitarnin stocks

3. Explants can be surface sterilized by

a)Dry heat b) Wet heat c) chernical r.nethodsd) Incineration

4. Pollen culture technique is used to produce

a)Hybrid plants b)Haploid plants c)Diploid plants d) a, b, c are corcct

5. Cytoplasmic hybrids containing nucleus from one parent and cytoplasrn frorn both
parents are called

a)Asymrnctric hybrids b) cybrids c) Somatic hybrids d) Syrnrnetric cybrids

6. The tissue culturing can be used to produce

a) Calius cultures b) Ilybrid plants c) Patl.rogen-free plants d) a,, b' c ate

corect
7. The horrnones uscd in p.lanl tissue oulture inciudc
a)lAA b) Gibberellins c) AI3A and IBA d) a, b, c are true

8.In autoclave, the media is generally sterilized at 121oC having pressure at

a) 10 psi b) 15 psi c) 20Psi d) 25Psi

9. Chernical lirsion o{'protoplast do lx)t rcquit'e

a) PEG treatment b) NaNO; treatmentc) ACiDC treatment d)Calcium treatment

I 0. Miorollltration involves
a) microporous mediumb) tnembrane filters c) r-nicrofilters d) a, b, c are true
 T]NIVERSITY OF THE PUNJAB aaa aaaaaa aaa a aa aa aaaa aaao
Sixth Semester - 2018 .a
Examination: B.S. 4 Years Programme !toonoltNo. ..................... i
ao t ao
aaaaaa aaooaaoaootl

PAPER: Palynology (Advance Course) TIME ALLOWED: 2 Hrs. & 45 Mints.

Course Code: BOT-317 Part - II MAX. MARKS:50

Attempt this Paper on Separate Answer Sheet provided'

questions' (10x2=20)
Q.2 Answer the following short
Grain'
l. Differentiate between a Spore and a Pollen
ll. Write a note on Environmental Palynology'
lll. Differentiate between Palynodebris ancl Varia'
lV. How will you identify Recycling?
the help of neat and labeled
V. Describe Pollen wall stratification with
diagramme
Vl. Describeapplicationsof Palaeopalynology
and
Vll. How would you differentiate between long Ranging
lndex

playnomorPhs?
indicate and why does it vary?
Vlll What does the colour of fossi! palynomorphs
and pollen:SaO' Scz' Pc3' Pv2'
lX. What do these codes indicate about spores
Exine'
X. Differentiate between Baculate and Echinate

Answer the following Long Questions' (1013=30)

procedure of Collection and

Q.3: Define Maceration. Describe in detail complete
isolation of Pollen / Spores'
Maceration of flowers from different plants for the
(10 Marks)
of sporopollenin and
Q.4: a) Draw a diagram representing the alteration
by geothermal alterations
other organic substances with coalification
(05 Marks)
(adopted from Potonie and Kremp' 1953)'
for Pollen and Spores'
b) Describe the Turmal System of classiflcation
(05 Marks)
in sediments' (05 Marks)
Q.5: a) Discuss preservation of Spores and Pollen
used in Palynology?
b) How many different types of Mounting Media are
Write down the procedure to prepare Glycerin
Jelly' (05 Marks)
 IJNIVERSITY OF THE PUNJAB
Sixth Semester - 2018
. RolINo.
Examination: B.S. 4 Years Prosramme
\
PAPER: Palynology (Advance Course) TIME ALLOWED: 15 Mints.
Course Code: BOT-317 Part- I (Com MAX MARKS: 10
Affemnt this Paper on thic

back after expirv of time limit mentioned above.

Select the correct option and encircle it' (lxI0=10)

Q.1
l. Which is not included in palynomorphs
a.Nanofossils b. Dinoflagellates c' Scolecodonts d' Chitinozoan
parts of organisms
ll. Palynomorphs are organic walled whole organisms or
range in size from
a. 5um-200um b' 5um-300um c.5um-500um d.5um-50um

lll. Fossilized algae include

a. Spirogyra b- Padiastum c' Sargassum d. Chara

lV. Archeological palynology is the study of pollen from

a. Ancient sediments b. Old civilizations c' Honey d. Crime Scenes

V. Carbonized sporopollenin is more resistant to

c. Reduction d. Polymertzation
a. Break down b' Oxidation
Vl.ThechancesoffindingfossilPollenandSporesismorelikelyin
a.Coarsegrainedsandstoneb'Finegrainedsandstonec'Siltstoned'
claY stone
VIl. Angiospermic pollen are abundant in
a. Permian sediments b' Tertiary sedimenis c. Jurassic sediments

Vlll. According to Faegri Foot Layer is included in

a. Ektexine b. Endexine c. Tectum d. None

lX. Elements irregularly distributed and undulating are called

a. Rugulate b. Striate
c' Reticulate d' Scabrate
The discordant forms that are younger than the associated rock
are
x.
characterized bY
a. Stratigraphic Leak b. Recycling c. Reworking d. None
 UNIVERSITY OF THE PUNJAB

PAPER: Air Pollution its Impacts and Control TIME ALLOWED: 2 hrs. & 30 mins.
Course Code: BOT-401 MAX. MARKS: 50

Attempt this Paper on Separate Answer Sheet provided

Q 2. Write short notes of 8 to 10 lines on the following: 20

(i) Sources and impacts of mercury pollution

(ii) Urban Pollution Rankings in Pakistan and other Countries
(iii) Effects of Chernobyl Event
(iv) Fa llout Problem
(v) Effects of Air Pollutants on lnert materials
(vi) Ilifferentiate between prlmary and secondary pollutants
(vii) Comparative radio-se n s itivity of different organisms
(viii) Fate of Radionuclides in the environment

Q 3. cive a detailed account of Acid Rain, its formation, types and different sources,
Discuss its effects on forests, aquatic ecosystems and artifacts. 10

Q 4. what is Greenhouse Effect, Global warming and climate change? Give an account of
'^ culprit gases and their warming potential. what are impacts of climate change? 10

Q 5. Give an account of
a) Exhaust Emissions from Motor Vehicles
b) Stratospheric Ozone, lts importance, Production and Depletion
 .....
UNIVERSITY OF THE PUNJAB .. RollNo.
Seventh Semester 2018
\\
\\
\
PAPER: Air Pollution its Impacts and Control TIME ALLOWED: 30 mins*.'..
Course Code: BOT-401 MA)L MARKS: 10

Attempt this Paper on this Question Sheet only.

q1. Encircle correct option of the statements gi'ren belov,r. 10

(i) Which one is an example of natural air pollution?

(a) lndustrial emissions (b) Volca nic Ash (c) Vehicle Exha ust (d) Cigarette smoke

(ii) Particulate air pollution from automobile exhaust is due to

'Zinc
(a ) (b) Cadmium (c) Lea d (d) Mercury

(iii) Acid p recip itatio n causes

(a) Human Skin Burn lb) Weathering of Rocks

(c) Changes in soil for plants growth (d) Changes in lakes for aquatic life

(iv) The major source of carbon monoxide emissions is

(a) Automobiles (b) Tobacco Smoking (c) lndustry (d) Domestic Fuel Burning

(v) The law that regulates air pollution is

(a) Air quality Control (b) Atmospheric Pollution (c) Oh No! Ozone Hole (d) Clean Air Act

(vi) Which of the following group of organisms is more sensitive to radiations

(a) Plants (b) Mamr-nals (c) lnsects (d) Amphibians

(vii) People who are least likely to be bothered by air pollution are

(a) Elderly people (b) Healthy People (c) lnfants (d) People with Asthma

(vili) Visible ozone injury symptoms on leaves of sensitive plants is

(a) Flecking (b) Stippling (c) Leaf Deiormation (d) All a, b and c

(ix ) Radiation that can travel only a few centimeters in the air is

(a) Beta Particles (b) Alpha Particles (c) Gamnra Rays (d) X-rays

(x) The phenomenon of Acid Rain is

(a) Global (b) Local (c) Regional (d) National

 UNTVERSITY OF THE PUNJAB
aa
aaaaa aa aa aa a aa aa aa aa aa aa

Seventh Semester 2018 : :

Examin ation : B. S. 4 Years Prosram me I .T:l.IJ:: ;:'; :'j:'.':'.': ;:'i'. .:

PAPER: Biohazards, Biosafety' Bioethics TIME ALLOWED:2 hrs. & 30 mins.

Course Code: BOT-403

Attempl this Paper on Separate Answer Sheel provided

PART II -SUBJECTIVE (50 N{ARKS) Tinre allowed: I'rl'o lIours antl lSminutes

I SHORT QUESTIONS (20- Marhi) i

Q. 2. Ansrver the following questions briefly.

i ,, , '
1. What is diflerence between animal ano piant cell cultule?
.

2. What is the Liroral 4nd bthical valLre ol hr-rman being clonirg?

l. Wr.ite clOwn tl,..c classitl czit i6_t 6i- lab6r,a Lor-ies grr tlrr- basrs 9i [rittsafcty [cvel.
4. \\;hrt ls tlrr: r'ojc oi biologicrtl \\ 3r.rilJr r:il ,

5. What are manmade cornpor"rrds? l

6. Describe'biiefl}, 11i.'l causcs a-ncl effbcts odgreenhouse efl'eut.

7, Hou' can we get a Patent? . ,

, l,
8. What are thb rlehsures rvhich are essentially ncedecL fol the protection of factory
worl.lels? ' t: ,

9. ,, , : the rnost dargeL'ous Solid

Describe
, :;, I rr,.
Wastei ,
I

10. Hor.r'cair we plepare a standarcl labortoly?

Q. 3. Ansrver the following tluestions, (3 0)

;.
.' i : ,.; I

iii. (a) \Vlnt is Genetic errginccr inu'? (05 r\'larl<s)

(b) Write dolvn a sllorl. note on Heavy nretals;?

:l
. (0-5 Nlarks)
l.l
tv. (a) What are different types of miclobes u,hich canse hazardolrs effect on environment?
. , i i: , .,, . (05 Marks) "

(b) \&ritea)lroltnoteon'WatelPolhrrion' (Mart<s: 5)

111. (a) What ale solid waste? Cive measures to discard it. (05 Marks)

(b) What is the role of Environmental Frotection Agency?

(05 }Iarks)
 UI\TIVERSITY OF TI{E PUNJAB '-.
Roll No.
Seventh Semester 2018
Examination: B.S. 4 Years Proeramme

PAPER: Biohazards, Biosafety, Bioethics TIME ALLOWED: 30 mins]'.

Course Code: BOT-403 MA)L MARKS: 10

Attempt this Paper on this Question Sheet only.

Attcnrpt all qucstions, Marlis shall'be clecluctcil I'or rvrong sp:cllings, ernsing or
overrvriting. Write your narncs and roll no. op nll slrce{s.

T I CTIVE (10 MARKS e allor

Q. l. Multiple choice questions: Tick (J; thc coircct flnsrvcr. (10 Marks)

l.Sor.neofthediffelencesbetu,eenafum'.hoodanci abiological safetycabinet(BSC)are

that a BSC plotects botl.r the user and the nratei"ial insicle the cabinet and that the exhaust
is HEPA filtered.
'l
t Lrc ol I'alsc

7. Which olthe lbllowins

'.'
'1r1g.pr
g practiccs
j'r
shoLrld be utilized when r'r,orliirrg in a biological
,,.j, i'
s at-etv
c-ab inet??
A. Disinfect.the wolk sultace olthe BSC befole ancl aiter work

' A.'Wear a lab coat ancl iranspolt nrateli.r[s irt r.rLrr pocket
B. Wear gloves and carries the nrarelill iLr 1*rLrl harrcis
C. Scat rnaterials irr a leak-prorrl'. shatter'-r'esistant iecor]dar-1'conlainer
D..Cej]sirr:c.,e[1cr'rltLrretlas[Sanddishesareif'l:ile1brtralispor.t
$. The watel hoclies in distlict l(asirr ale contamiuated by
,.,
(b) Glass (b) PapeL (c) Tar.rneries (d) l-lospitril waste

(b) Ilear (b) ltadiation (cl

,], CFC (D) Pb
? .Female nrosqr.rito take blood because it need ---------r------------ for egg production
(b)Proteirl,(b)fa1s,,(c)Carliohyc1rates:(d)VitanriIis
g. The Drocess bv which:all livinecells. sooreS. and acellulal entities ate eith
lernoved fronr an obiect or habitat is called ', i

(b) Antisepsis (b) Di'sinfcction (c) Sianitation (d) Sterilization

' :
i . irii ,, ,' ,', : ir.l,t I.
: :.
1. " 1,.
.::
,

l: : ' :

I Ultravioler (UV) r'adiation is an effectiVe microbiaI contlol agent because

(b) It oxi<lizes cellLli4i constituei-rts (b)llt damiges DNA l

1c) lt tlanrages
s tlrc: cell nicurbrane (d) Alt o1'tlrc abor u
. ,,,,
YY rrrur.rI apcnc'
clSvrt\/J _Li lr,JPvrlntu!r, rur ru6LrroLrrrS !rloIrrrv\/r@rrrr r

(a) Envilonmental Prbtection Agencl, (b) Natirorial hr-stitirtes oI l]eaitlr

,:i

ic) Footl arrd Di ug Adurinistratic,n (tl; \.['oilcl ] lcnlth ()r'ganization

 ....
T]NIVERSITY OF THE PfINJAB Roll No.
Seventh Semester 2018

\
PAPER: Plant Nutrition and Soil Fertility TIME ALLOWED:30 mins.'.
Course Code: BOT-405 MA)L MARKS: 10

Attempt this Paper on this Question Sheet only.

10
Q1. Encircle correct option of the statements given below.

(i) Which of the following is a micronutrient

(a) Co (b)Cd (c) P (d)Zn

(ii) Dark Green or Blue Green foliage is typical deficiency symptom of

(a) Phosphorus (b) Manganese (c) Potassium (d) a and c

(iii) Modified Hoagland Solution contains

(b) Onlv Nitrate (b) Nitrate and Ammonium (c) Nitrite (d) Both b and c

(iv) which one of the following is a preferred Nitrogen Source to support cell d ivision in meristematic tissues

(a) Nitrite (b) Nitrate (c) Ammonia (d) Ammonium

(v) Giavel has pa rticle size

(a) 0.2 mm - 2 mm (b) 0.02 mm - 0.2 mm (c) > 2 mm (d) 0 002 mm - 0.02 mm

(vi) Mycorrhizae is a Greek word for

(d) Hearting Net
(a) Bacteria and Fungi (b) Fungus and Root (c) Nodule forrnation

(vii) What is pcrcentage proportion of Nitrogen fixed by Microorganisms

(a ) 50o/o (bl20% lcl ao% (d)eo%

(vlii) Physical loss of gaseous Ammonia to the atmosPirere is called

(a) Mlneralization (b) Nitrification (c) Volatilization (d) Ammonification

co-enzymes
(ix ) Which nutr ent is considered as constituent of Nucleic Acid, NucleotideS and

(a) P (b)si (c) N (d)s

(x) Ha rd, dry and brittle plant tissues with dlstorted leaves are deficiency symptoms of

(a) Molybdenum (b) Boron (c) copper (d) lron

 T'NIYERSITY OF TI{E PUNJAB
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Seventh Semester 2018 aa
Examination: B.S. 4 Years
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non No. ..................... i

PAPER: Plant Nutrition and SoiI Fertility TIME ALLOWED: 2 hrs. & 30 mins.
Course Code: BOT-405 MAX. MARKS: 50

Attempt this Paper on SE arate Answer Sheet provided

20
Q 2, Write short notes of 8 to 10 lines on the following:

(i) Write down the chemical composition of Hoagland culture solution

(iii Functions oi l\itrogen and Magnesium in plants
(iii) The concept of the EcotYPe
(iv) Beriefits of use of Lime in agriculture
(v) Effects of plant nutrients on their water requirements

absorption of Nitrate and

Q 3. What are experimental and biological considerations regarding
10
Ammonium ions? Give a detailed account of mechanism of absorption.

of the physiology of the formation of

Q 4. What is Nitrogen fixation? Give a detailed account
10
root nodules.

10
Q 5. Give a critical account of role of mineral elements in plant ecological studies.
 UNTYERSITY OF TIIE PUNJAB
2018
f '''""'r,."""""""
seventh semester
Examination : B.S. 4 Years Prosramme i .l:tJ.\:...;: ;: ;:.j:...:... :.:.. ;

PAPER: Biodegradation and Bioremediation TIME ALLOWED:2 hrs. & 30 mins.

Course Code: BOT-407 MA)L MARKS: 50

Attempt this Paper on Seltorate Answer Sheelprovided.

SIIORT QIIESTIONS (20 MARKS)

Q.2 Answer the following questions briefly:

1. What do you mean by lithotrophic bacteria?
2. Write down the basic procedure for biorernediation?
3. What are Xenobiotic?
4. What is ACTIVATION? Enlist mechanisms involve in ACTIVATION.
5. What is meant by Biodegradation?
6. Describe the process ofsorption and its effect on biodegradation.

7. What are heavy metal compounds? How can they enter in our environment?

8, What is meant by bioreactor'based method of bioremediation?

9. What are important life cycle for a good environment?
10. Explain the environmental effects on bacterial growth.

Q. 3 Answer the following questions (30 Marks)

i. (a). What is meant by enrichment culture? What type of enrichment shategies

may be used for pollution contol? (05 Marks)

(b). Write down the major source of environmental pollution. (05 Marks)

ii. (a). Describe different strategies used for bioremediation with special
emphasize on genetic approach. (05 Marks)

(b). Write a note on biocatalyst selection for bioremediation. (05 marks)

iii. (a). Describe the effect of different environmental factor on Biodegradation

and bioremediation? (05 Marks)

(b). Describe Microbial Technology for removal of heavy metals from

environment. (05 Marks)
 UNTVERSITY OF TITE PI.II\JAB.". RoIl No.
Seventh Semester 2018
Examination: B.S. 4 Years Prosramme

PAPER: Biodegradation and Bioremediation TIME ALLOWED: 30 minsl'..

Course Code: BOT-407 MAX. MARKS: 10

Attempt this Paper on this Question Sheet only.

Q. I Multiple choice questions: Tick ( / ) the correct answer:

(10 Marks)

I . The use of bacteria to degrade environmental pollutants is known as:

a). Microremediation b). Nanoremediation

c). Bioremediation d). All of these

2. Microorganisms remove organic compounds by:

a).Adsorption b)' Degradation
c). Complexion d). AII of these

3. A non-directed physio-chemical interaction between heavy metals and surface of

microorganisms is called :

f. Siotransformation b). Biomagnifications

c). Bioaccumulation d). Biosorption

4. Which of the following have bee used for bioremediation?

a). Aerobic Bacteria b). Plants
c). Horses d). Viruses

5. Generally, enzymes which can tansform organic pollutants are:

ui. Sp""ifr" b)' Non-sPecific
fU". high molecular weight d)' Resistant to high temperature
"). the procedure is known as:
6. The gene are use oftwo difference sources for a typical pathway,
a). Rational approach b)' Bioaugmentation
ci. Directecl Jvolutioo d)' Biostimulation

T.Thedecomposerwouldmostlikelyoccuratwhichstageofwastewatertreatnent?
a).Primary b)' SecondarY
c). tertiarY d)' Advanced

8. The catabolic gene are usually present on:

a). Plasmids b). Chromosome
c). Ribosome d). None of these

9. Plant microbe interaction use for:

a). Improvement of Plant Yield b). To make association for environment
c). To take strelter d). None of these

10. Microorganisms remove metals by:

ai. AdsorPtion b)' Degradation
c). ComPlexion d)' A1l of these