Tryptone, Certified (Casitose, Certified) CR014
Intended use
Tryptone, Certified (Casitose, Certified) is manufactured by a controlled enzymatic hydrolysis of milk protein. It is
rich in tryptophan and used by a wide variety of microorganisms, recommended in a number of media such as
Sterility Testing media, Diagnostics Media, media for biochemical characterization etc.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face
protection. Follow good microbiological lab practices while handling specimens and culture. Safety guidelines may
be referred in individual safety data sheets.
Limitations
1. It is biological origin product since variation in colour of powder and clarity may observed.
2. Each lot of the product has been tested for the organisms specified on the COA. It is recommended to users
to validate
the medium for any specific microorganism other than mentioned in the COA based on the user’ s requirement.
3. Individual organisms differ in their growth requirement and may show variable growth patterns on the medium
prepared by the product.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when
stored at recommended temperature
Quality Control
Appearance : Off white to light yellow homogenous free flowing powder characteristic odour but not
putrescent
Solubility : Freely soluble in distilled/purified water, insoluble in alcohol and ether.
Clarity : 1% w/v aqueous solution is clear without any haziness after autoclaving at 15 lbs pressure (121ºC)
for 15 minutes.
pH : pH of 2% w/v aqueous solution at 25ºC 6.3 - 7.3
Microbial Load :
Bacterial Count : <= 2000 CFU/gram by plate method, when incubated at 30-35ºC for not less than 3 days
Yeast & mould Count : <= 100 CFU/gram by plate method, when incubated at 20-25ºC for not less than 5 days.
Test for pathogens : 1. Escherichia Coli- Absent/gram of sample 2. Salmonella species- Absent/10 gram of
sample 3. Pseudomonas aeruginosa - Absent/gram of sample 4. Staphylococcus aureus - Absent/gram of sample
5. Candida albicans - Absent/gram of sample 6. Clostridia - Absent/gram of sample
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Please refer disclaimer Overleaf.
HiMedia Laboratories Technical Data
Indole Test : Tryptophan content: Passes
Cultural response : Cultural response observed after an incubation for bacterial at 35-37ºC for 18-24 hours and
for fungal at 20-25°C for not less than 5 days by preparing Soyabean Casein Digest Medium (M011) using
Tryptone Certified (Casitose, Certified) as an ingredient.
Cultural Response
Organism Growth
Staphylococcus aureus subsp. aureus luxuriant
ATCC 25923 (WDCM 00034)
Staphylococcus aureus subsp. aureus luxuriant
ATCC 6538 (WDCM 00032)
Escherichia coli ATCC 25922 (WDCM 00013)luxuriant
Escherichia coli ATCC 8739 (WDCM 00012) luxuriant
Pseudomonas aeruginosa ATCC 27853 luxuriant
(WDCM 00025)
Pseudomonas aeruginosa ATCC 9027 luxuriant
(WDCM 00026)
Bacillus subtilis subsp. Spizizenii ATCC luxuriant
6633 (WDCM 00003)
Salmonella enterica subsp. enterica luxuriant
Typhimurium ATCC 14028 (WDCM 00031)
Salmonella enterica subsp.enterica serotype luxuriant
Abony NCTC 6017(WDCM 00029)
Kocuria rhizophila ATCC 9341 luxuriant
Streptococcus pneumoniae ATCC 6303 luxuriant
Candida albicans ATCC 10231 (WDCM luxuriant
00054)
Aspergillus brasiliensis ATCC 16404 luxuriant
(WDCM 00053)
Chemical Analysis :
Total Nitrogen : ≥11.00 %
Amino Nitrogen : ≥4.00 %
Sodium chloride : ≤5.00 %
Loss on drying : ≤5.00 %
Residue on ignition : ≤15.00 %
Storage and Shelf Life
Store between 10-30°C in tightly closed container and away from bright light. Use before expiry date on label. On
opening, product should be properly stored in dry ventilated area protected from extremes of temperature and
sources of ignition. Seal the container tightly after use.
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Please refer disclaimer Overleaf.
HiMedia Laboratories Technical Data
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product.
Follow established laboratory procedures in disposing of infectious materials and material that comes into contact
with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques.
Storage temperature Do not use if package is damaged
HiMedia Laboratories Pvt Limited
C-40,21/Y, MIDC, Wagle Ind Area
Thane(W)–400604,Maharashtra,India
Revision : 08/2022
Disclaimer :
User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in
this and other related HiMedia™ publications.The information contained in this publication is based on our research and development
work and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes to
specifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use but
for laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified.Statements contained herein should
not be considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.
.HiMedia Laboratories Pvt. Ltd. Reg.Office : Plot No:C-40, Road No: 21Y, MIDC,Wagle Industrial Page : 3 of 3
Area,Thane(West)-400604,Maharashtra,INDIA.
Tel:00-91-22-61471919/61169797/69034800,Fax:00-91-22-61471920.
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