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DNA Replication - Labeling

1. The document is a worksheet about DNA replication that requires labeling structures and enzymes involved and ordering the steps of replication. 2. Key terms to label include helicase, Okazaki fragments, leading strand, DNA primase, single strand binding proteins, DNA ligase, DNA polymerase III. 3. The main steps of replication are that helicase unwinds DNA, primase creates an RNA primer, polymerase III adds nucleotides, primers are replaced with DNA, and ligase joins Okazaki fragments.

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5K views1 page

DNA Replication - Labeling

1. The document is a worksheet about DNA replication that requires labeling structures and enzymes involved and ordering the steps of replication. 2. Key terms to label include helicase, Okazaki fragments, leading strand, DNA primase, single strand binding proteins, DNA ligase, DNA polymerase III. 3. The main steps of replication are that helicase unwinds DNA, primase creates an RNA primer, polymerase III adds nucleotides, primers are replaced with DNA, and ligase joins Okazaki fragments.

Uploaded by

hpctrw8fh8
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOCX, PDF, TXT or read online on Scribd

Name:____________________________________________ Date:_________

DNA Replication - Labeling (with word bank)

DNA polymerase III 3’ 5’ DNA Ligase Okazaki fragment DNA Primase


Single Strand Binding Proteins Helicase Leading Strand Lagging Strand

Identify the structure


1. _____________________________ Enzyme that unwinds DNA
2. _____________________________ Fragments of copied DNA created on the lagging strand
3. _____________________________ The strand that is copied in a continuous way in the 5’ to 3’ direction
4. _____________________________ Joins Okazaki fragments
5. _____________________________ Builds a new DNA strand by adding complementary bases
6. _____________________________ Stabilizes the DNA molecule during replication
7. _____________________________ Strand that is copied discontinuously because it is traveling away from helicase
8. _____________________________Initiates the synthesis DNA by creating a short RNA segment at replication fork

9. Place the events in the correct order:


____ DNA polymerase III adds nucleotides in the 5’ to 3’ direction
____ DNA polymerase III attaches to the primer
____ Okazaki fragments are joined together by ligase
____ DNA helicase unwinds DNA
____ DNA primase creates an RNA primer
____ DNA polymerase I replaces the RNA primer with DNA

10. Why is replication called “semi-conservative?” ___________________________________________________

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