BIO462
BIOCHEMISTRY
LAB REPORT 1 : DILUTION AND
MOLARITY
NAMES MATRIC NUMBERS
CORLAINE SHIERY ANAK JAMES 2023899958
FATIMAH BINTI HASRIN 2023491958
NURFARAH NABIHA BT AHMAD RASDAN 2023492024
NUR SHAZLIYANA BINTI HASRIN 2023638078
NUR FATIHAH BINTI MOHD RAKIDIN 2023414874
TITLE : EXPERIMENT 1 :DILUTION AND MOLARITY
QUESTIONS
i. How would you prepare :
a) 10 ml of a 1:10 dilution of a 1M NaCl solution and what would be the final
concentration of NaCl.
M1V1 = M2V2
(1𝑚𝑙)(1𝑚𝑙)= 𝑀2(10𝑚𝑙)
(1𝑚𝑙)(1𝑚𝑙)10𝑚𝑙 = 𝑀2
𝑀2= 0.1 𝑀 𝑁𝑎𝐶𝑙
b) 80 ml of a 1:20 dilution of a 1M NaCl solution?
M1V1 = M2V2
(1𝑚𝑙)(4𝑚𝑙)= 𝑀2(80𝑚𝑙)
(1𝑚𝑙)(4𝑚𝑙)80𝑚𝑙 = 𝑀2
𝑀2= 0.05 𝑀 𝑁𝑎𝐶𝑙
c)50 ml of a 1:25 dilution of a 1M NaCl solution.
M1V1 = M2V2
(1𝑚𝑙)(2𝑚𝑙)= 𝑀2(50𝑚𝑙)
(1𝑚𝑙)(2𝑚𝑙)50𝑚𝑙 = 𝑀2
𝑀2= 0.04 𝑀 𝑁𝑎𝐶𝑙
ii. How would you prepare exactly 6 ml of a 1/20 dilution (assume the concentration of your
starting solution is “1”)?
M1V1 = M2V2
(1𝑚𝑙)(0.3𝑚𝑙)= 𝑀2(6𝑚𝑙)
(1𝑚𝑙)(0.3𝑚𝑙)6𝑚𝑙 = 𝑀2
𝑀2= 0.05 𝑀 𝑜𝑓 𝑥
iii. You are provided with an antibody solution (Ab) that has a concentration of 600
micrograms (µ)/microliter (µL). For a lab, it is necessary to make the following dilutions.
a) 10µL of 600 µg/µL Ab + 190 µL of butter to make a 1:20 dilution at 30 µg/µL.
M1V1 = M2V2
(600)(10)=𝑀2(200)
𝑀2=(600)(10) / 200
𝑀2=30 µ𝑔/µ𝐿
b) 20µL of 1:20 Ab + 40 µL of buffer to make a 1:60 dilution at 10 µg/µL.
M1V1 = M2V2
(30)(20)= 𝑀2(60)
𝑀2=(30)(20) / 60
𝑀2=10 µ𝑔/µ𝐿
c) 5µL of 1:60 Ab + 5µL of buffer to make a 1:120 dilution at 5 µg/µL.
M1V1 = M2V2
(10)(15)= 𝑀2(10)
𝑀2= (10)(5) / 10
𝑀2= 5 µ𝑔/µ𝐿
∴ 5/600 = 1/120
= 1: 120 𝑑𝑖𝑙𝑢𝑡𝑖𝑜𝑛
d) 10 µL of 1:60 Ab + 90 µL of buffer to make a 1:600 dilution at 1 µg/µL.
M1V1 = M2V2
(10)(10)= 𝑀2(100)
𝑀2= (10)(10) / 100
𝑀2= 1 µ𝑔/µ𝐿
∴ 1/600 = 1: 600 𝑑𝑖𝑙𝑢𝑡𝑖𝑜𝑛
e) 10µL of a1:60 Ab + 40 µL of buffer to make a 1:300 dilution at 2 µg/µL
M1V1 = M2V2
(10)(10)= 𝑀2(50)
𝑀2= (10)(10) / 50
𝑀2= 2 µ𝑔/µ𝐿
∴ 2600 = 1300
= 1: 300 𝑑𝑖𝑙𝑢𝑡𝑖𝑜𝑛
f) 10µL of 1:60 Ab + 10 µL of a buffer to make a 1:120 dilution at 5 µg/µL.
M1V1 = M2V2
(10)(10)=𝑀2(20)
𝑀2=(10)(10) / 20
𝑀2= 5 µ𝑔/µ𝐿
∴ 5/600 = 1/120
= 1: 120 𝑑𝑖𝑙𝑢𝑡𝑖𝑜𝑛
iv. How much 2.0 M NaCl solution would you need to make 250 ml of 0.15 M NaCl
solution?
M1V1 = M2V2
(2.0)𝑉1= (0.15)(250)
𝑉1= (0.15)(250) / 2.0
𝑉1=18.75 𝑚𝑙
v. What would be the concentration of a solution made by diluting 45.0 ml of 4.2M KOH to 250
ml?
M1V1 = M2V2
(4.2)(45)=𝑀2(250)
𝑀2=(4.2)(45) / 250
𝑀2= 0.756 𝑀
vi. What would be the concentration of a solution made by adding 250 ml of water to 45 ml of a
4.2M KOH?
M1V1 = M2V2
(4.2)(45)=𝑀2(295)
𝑀2 = (4.2)(45) / 295
𝑀2 = 0.641 𝑀
vii. How much 0.20M glucose solution can be made from 50.0 ml of 0.50M glucose solution?
M1= 0.50 M
V1 = 50.0 mL
M2 = 0.20 M
V2 = x
M1V1 = M2V2
(0.50) (50.0) = (0.20) (x)
x = 125mL
V2= 125mL
viii. What is the molarity of a solution that has 4.5mol of solute dissolved in 300 ml of
solution?
Molarity = mol/volume in L
M = 4.5mol / 0.3L
M = 15M
Ix. What is the molarity of a solution of NaCl that has 0.491g dissolved in 400 ml of
solution?
𝑁𝑎𝑂𝐻 = 22.989769 + 17.008 = 39.9978 gmol-
𝑚𝑜𝑙𝑒𝑠 𝑜𝑓 𝑁𝑎𝑂𝐻 = 0.491 𝑔 / 39.9978 𝑔𝑚𝑜𝑙-
= 0.01228 𝑚𝑜𝑙
𝑚𝑜𝑙𝑎𝑟𝑖𝑡𝑦, 𝑀 = 0.01228 𝑚𝑜𝑙 / 0.4 𝐿
= 0.0307 𝑀
x. What is the molarity of a solution prepared by diluting 10.00 ml of a 4.281 M solution
to 50 ml?
𝑚𝑜𝑙𝑎𝑟𝑖𝑡𝑦, 𝑀 = 𝑚𝑜𝑙 / 𝑣𝑜𝑙𝑢𝑚𝑒 𝑖𝑛 𝐿
4.281𝑀 = 𝑚𝑜𝑙 / 0.01L
𝑚𝑜𝑙 = 0.04281
𝑚𝑜𝑙𝑎𝑟𝑖𝑡𝑦, 𝑀 = 0.04281𝑚𝑜𝑙 / 0.3 𝐿
= 0.8562 𝑀
