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Platelet preparation involves two centrifugation spins to separate components. The first light spin separates red blood cells and white blood cells from plasma and platelets. The second heavy spin forces platelets into a platelet concentrate bag. Platelets are stored at 20-24°C for up to 5 days with agitation and must contain at least 5.5 x 1010 platelets to increase a person's platelet count by 5000/μL. Platelets are used to help stop bleeding and form clots. Fresh frozen plasma undergoes processing where whole blood is centrifuged to separate plasma from red blood cells. The plasma is then frozen within 8 hours of donation and stored at -18°C or colder for up to 1

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189 views7 pages

Assignment

Platelet preparation involves two centrifugation spins to separate components. The first light spin separates red blood cells and white blood cells from plasma and platelets. The second heavy spin forces platelets into a platelet concentrate bag. Platelets are stored at 20-24°C for up to 5 days with agitation and must contain at least 5.5 x 1010 platelets to increase a person's platelet count by 5000/μL. Platelets are used to help stop bleeding and form clots. Fresh frozen plasma undergoes processing where whole blood is centrifuged to separate plasma from red blood cells. The plasma is then frozen within 8 hours of donation and stored at -18°C or colder for up to 1

Uploaded by

Amalina Rozani
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

Platlet Preparation of Platlet.

Require two spins - Light spin separates RBCs and WBCs from plasma and platelets - Heavy spin platelets in platelet rich plasma (PRP) will be forced to the bottom of a satellite bag . 40-60 mL of plasma is expelled into another satellite bag, while the remaining bag contains platelet concentrate

Storage Temperature - 20-24C for 5 days (constant agitation) Each unit should contain at least 5.5 x 1010 platelets Each unit should elevate the platelet count by 5000 L in a 165 lb person Important in maintaining hemostasis Help stop bleeding and form a platelet plug (primary hemostasis)

People who need platelets: Cancer patients Bone marrow recipients Postoperative bleeding

FRESH FROZEN PLASMA


Plasma that is frozen within 8 hours of donation -18C or colder for 1 year Processing 1. Whole blood FFP Can be processed manually or via newer, automated whole blood processing equipment Plasma separated from red cells via centrifugation, expressed from main bag into a separate bag o In the US, when not preparing whole-blood derived platelets, this is done in a refrigerated centrifuge (1-6 C) using a heavy spin (5000 x g) o Plasma is then expressed into a satellite bag Note that FDA requires if plasma has been inadvertently thawed. This is done by freezing with a rubber band that is then removed, or freezing units on their side and storing them upright

2. Apheresis FFP Apheresis equipment separates components (red cells, platelets, plasma) automatically No additional centrifugation steps are necessary Same thaw-detection methods are used as were outlined above.

Provides coagulation factors for Bleeding Abnormal clotting due to massive transfusion Patients on warfarin who are bleeding Treatment of TTP and HUS Factor deficiencies ATIII deficiency DIC when fibrinogen is <100 mg/dL

FFP is thawed before transfusion 30-37C waterbath for 30-45 minutes Stored 1-6C and transfused within 24 hours Needs to be ABO compatible

CRYOPRECIPITATE
Cryoprecipitated antihemophilic factor (AHF) or Cryo is the precipitated protein portion that results after thawing FFP Contains: o von Willebrands factor (plt. adhesion) o Fibrinogen 150 mg in each unit o Factor VIII About 80 IU in each unit Fibrinonectin Same storage as FFP (cannot be re-frozen as FFP once it is separated); -18 for 1 year

If thawed, store at room temp 4 hrs The leftover plasma is called cryoprecipitate reduced or plasma cryo Good for thrombocytopenic purpura (TTP) CRYO is used for Factor VIII deficiency (Hemophilia A) von Willebrands Disease Congenital or acquired fibrinogen defects (i.e., dysfibrinogenemia)

PRESERVATIVE OR ANTICOAGULANT IN BLOOD BAG


CPDA-1 (anticoagulant and storage of blood) Contain sodium citrate ,citric acid,dextrose and monobasic sodium phosphte in established concentrations. The CPDA-1 contains the extra ingredient of adenine. The citrate compound prevent coagulation by chelating calcium and the dextrose provides nourishments for the living red blood cells because the low temperature of storage so cells must have nutritive source to remain alive. The amino acid denine used in the production of the ATP

CPD and CP2D (anticoagulant and storage of blood) Contain sodium citrate ,citric acid,dextrose and monobasic sodium phosphte in established concentrations. Both CPD and CP2D have an FDA-approved shelf life of 21 days when product is stored at low temperature. Prevents coagulant of blood as Citrate ionchelates Calcium Nutrition source for red cell Adjusts pH

SAGM (red cell preservation) Contain sodium cloride ,dextrose, adenine and D-mannitol in established concentrations. Nutrition source for red cell Adjusts osmotic pressure Supports to maintain ATP level in red cells Supports integrity of red cell membrane (to avoid haemolysis)

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