Scribd
Upload
316 views7 pages

Exp 3

This document describes a procedure to determine the average diameter of starch samples from rice and potato. Key steps include calibrating an eyepiece micrometer using a stage micrometer, mounting starch powder, measuring the diameter of isolated starch grains under the microscope, calculating dimensions using the calibration factor, and reporting average diameter, range of sizes, and size distribution. The expected results are tables with the average diameter, highest and lowest diameters measured, and number of starch grains within given size ranges for each sample.

Uploaded by

hitayujain123
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
316 views7 pages

Exp 3

This document describes a procedure to determine the average diameter of starch samples from rice and potato. Key steps include calibrating an eyepiece micrometer using a stage micrometer, mounting starch powder, measuring the diameter of isolated starch grains under the microscope, calculating dimensions using the calibration factor, and reporting average diameter, range of sizes, and size distribution. The expected results are tables with the average diameter, highest and lowest diameters measured, and number of starch grains within given size ranges for each sample.

Uploaded by

hitayujain123
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
You are on page 1/ 7

Exp3

AIM-to determine the average diameter of given


starch sample
Reference-Dr KR khandelwal, DR Vrunda sethi,
“Practical pharmacognosy”, nirali prakashan pg-

Requirement-micrometer, eyepiece
micrometer,stage micrometer,starch
sample(potato starch, Rice starch)

Theory-
Micrometry-
It is a scale used to measure microscopic objects it
is expressed in micron.

The technique of micrometer evaluation is useful


for measurement of dimension of starch
grain,calcium oxalate crystals,fibres and cell
content in powdered or unground crude drugs.

The size of an object or part of it is can be


measured with help of 2 types micrometer
1-stage micrometer
2-eye piece micrometer
1-Stage micrometer:
It is a slide it contain a standard scale length of
1mm which is divided into 100 divisions

1 division of stage
micrometer=1/100mm=0.01mm

2-Eye piece micrometer


It is a circle of glass with a scale etched on the
surface. It is suitable for insertion inside the
ocular and during the operation of measurement.
A convenient form is a linear scale of 1mm divided
into 100 division

The value of its division varies with the


combination of eye piece and objective lenses.
Hence, calibration of one division of it is essential
during practical work it order to get exact value of
its one division in terms of microns

Callibration of one division of eyepiece


micrometer-
1-put the eyepiece micrometer on the
diaphragm of the ocular
2-put the stage micrometer on the
stage of microscope
3-See the lines of two micrometer
coincide count the number of lines
required to coincide

Characteristics of starch grain


-Rice starch
Nature- simple and compound
Shape-polyhedral with sharp angles
Size- 4 to 10u but average grains are about 6-4
compound grains may contain upto 150
components and may measure upto 30u in width
and 20u in length

Hilum and striations-no striations are visible and


only very occasionaly a hilum can be distinguished
as small dot.

-Potato starch
Nature-mostly simple but occasionally
Shape-subspherical or ovoid or often slight
flattened.
Size- Subspherical grains are upto 35u in length
and ovoid grains vary from 30 to 100u

Hilum and striations-The hilum occurs as a small


point situated towards narrow ends of grain and is
surrounded by well marked concentric striations.

Procedure
1-Calibrate an eyepiece micrometer using a stage
micrometer and calculate the factor.
2-Mount a little quantity of crude drug powder.
3-measure the diameter of isolated starch grain in
the mount by focusing them on the scales of
eyepiece micrometer. Note the number of
division covered by starch grains.
4-Calculate the dimension of 50 starch grains
multiply the division by the factor so as to get
actual dimension of starch grain in microns.
5-calculate the average value and give the range
for the dimension of starch grains.
Calculation-
1 division of stage micrometer=10um
3 division of eyepiece micrometer=5 division of
stage micrometer

now, 1 division of stage micrometer=10um

5 division of stage micrometer=5x10=50um

3 division of eyepiece micrometer=50um


1 division of eyepiece micrometer=50x1/3=16.66um

Observation table
SR No of Multiplication diameter
division factor
Result
The average diameter of given rice starch sample was
found to be _____

Highest diameter
Lowest diameter
Average diameter
Range of size

Size distribution No of particles

2- The average diameter of given potato starch


sample was found to be _____

Highest diameter
Lowest diameter
Average diameter
Range of size

Size distribution No of particles

You might also like