Date:
Expt. No.:
Oxygen Assessment in
Water Quality Monitoring: Total Dissolved Method
Experiment Different Water Samples by Winkler's
in water but
Dissolved oxygen (DO)is essentialto living organisms corrosion.
boiler
Problem definition
harmful if present in boiler feed water leading to
water.
Winkler's titration method is used to assess DO in
Methodology samples.
Solution Estimation of total dissolved oxygen in different water
Students will learn to
Student learning a) perform Winkler's titration method
different water samples
outcomes b) assess the total dissolved oxygen in
useful in studying corrosion
Principle: Estimation of dissolved oxygen (DO) in water
pollution. DO is usually determined by
effect of boiler feed water and in studying water
DO oxidize potassium iodide (KI)
Winkler's titration method, which is based on the fact that
molecular state, as such it cannot oxidize KI. Hence,
to iodine (I2). Since DO in water is in
and
to bring about the reaction between KI
manganese hydroxide is used as an oxygen carrier
on MnSO4.
Oxygen. Manganese hydroxide, in turn, is obtained by the action of NaOH
MnSO, + 2NaOH Mu (OH), + Na, SO4
2Mn(OH), +0, ’ 2MnO(OH),
MnO(OH), +H,S0, ’ MnSO, +2H,0+[O]
2KT +H,SO, +[0]-K,S0, +H,0+1,
2Na, S,O, +1, ’ Na,S,O, + 2Nal
Starch + Ih ’ Blue colored complex.
thiosulphate (NaS>O;) solution
The liberated iodine (l2) is titrated against standard sodium
using starch as indicator.
Requirements:
potassium dichromate (O.01 N), sodium
Reagents and solutions: Standard buffer of pH 7, standard
conc. HSOa.
thiosulphate solution, 10% KI solution, alkali KI solution (KI + KOH in water),
manganese sulphate, starch solution as indicator.
Apparatus: Conical flask, Burette, Measuring flask, Beakers.
7
Procedure:
Titration l: Standardization of Sodium Thiosulphate
Rinse and fillthe burette with given sodium thiosulphate solution (Bottle B). Pipette out 20 mL of
0.01N KCr:07 solution (Bottle A) into a clean conical flask. To this, add 5 mL H;SO, (1/2 TT), 5
ml (1/2 T.T) of 10% KI,and titrate against sodium thiosulphate solution. When the solution becomes
straw yellow colour, add starch indicator and continue the titration. End point is the disappearance of
bluish brown colour. Repeat the titration to get concordant value.
Titration 2: Estimation of Dissolved Oxygen
Using a measuring cylinder, add 100mL of sample water in a conical flask. Further, add 2 mL of
MnSO, and 2 mL of alkali KI solution and shake well for the rough mixing of the reagents. Set aside
the flask for few minutes to allow the precipitate to settle down, and then add 2 mL of conc. H2SO,
for complete dissolution of the precipitate. Then, titrate against std. sodium thiosulphate solution.
When the solution turn into light yellow, add starch indicator. End point is the disappearance of bluish
brown colour. Repeat the titration to get the concordant value. Calculate the strength of dissolved
oxygen from the titer value. Based on that, calculate the amount of DO in the given water sample.
OBSERVATION AND CALCULATIONS
Titration - I: Standardization of Sodium Thiosulphate
Volume of K;Cr0,
Burette reading (mL) Volume of sodium
S. No.
(Vi, mL) Initial Final thiosulphate (V2, mL)
1. 20 ml 20 20
2. 20 ml 20.5 20:5
3.
Concordant value
20:25
Calculations:
Volume of potassium dichromate VË = 20 mL
Strength of potassium dichromate N, = 0.01 N
Volume of sodium thiosulphate V;....:...mL (From Titration - 1)
Strength of sodiumthiosulphate N,= 0:0.0 I 7W
V,N, =VN:
.:. N,=V,N/V2
Strength of sodium thiosulphate = N= 20 x 0.0I/V, =.0:9.0.38.7.N
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Titration - II: Estimation of Dissolved Oxygen
Volume of water sample Burette reading (mL) Volume of sodium
S. No.
(Vi, mL) Initial Final thiosulphate (V2, mL)
1 100 ml
2. 100 ml
15:5 15:5
3.
Concordant value
L525
Caleulation:
Volume of sodiumthiosulphate V; dS:2nL (From Titration - 2)
Strength of sodium thiosulphate N=0:99. SS.J.N (From Titration - 1 calculation)
Volume of water sample taken V= 100 mL
Strength of given water sampleN=?
VN,= VN2
NË= V½X N/100
-.0.:Q0..5..
Amount of DO (ppm) normality x equivalent weight of 0, x 1000 mg/L of the given water sample.
=..Q.:00.!5..N x 8 x 1000 mg/L
-2:.04..ppm. o2| 3
Result: Amount of DO in the given water sample =...!....ppm.
