QUALITY PURIFIES.
Lewatit® ion exchange resins
and adsorbents for capture and
purification of biomolecules
ABOUT LANXESS
We are a leading specialty chemicals company based in Cologne, Germany,
well established on the global market. Our primary expertise lies in produc-
ing, developing, and marketing chemical intermediates, additives, specialty
chemicals, and plastics. As a specialist and efficient partner, we offer
solutions to all kinds of challenges faced by our customers. We focus on our
customers’ requirements in order to drive progress and reliably provide inno-
vative product, material, and service solutions. Our manufacturing, adminis-
tration, and logistics processes are designed for efficiency and performance.
We offer a broad range of technologies and solutions for the treatment of
water and other liquid media and are one of the leading manufacturers of ion
exchange resins, with production sites in Germany and India. Our Lewatit®
ion exchange resins and adsorbers are applied in many different industries
and applications to treat water and other liquid media.
With our sustainably produced Lewatit® Scopeblue ion exchange resins,we
offer products that have a carbon footprint that is up to 67 percent smaller
than products manufactured from conventional fossil sources and consist of
more than 90 percent renewable raw materials. In accordance with the mass
balance approach, they are chemically identical to conventional products
and are produced in the same plants using the same processes.
In addition, we also offer a range of Bayoxide® iron oxide adsorbers for vari-
ous water-treatment applications. Furthermore, our unique calculation and
design software LewaPlus® is used for modelling and dimensioning of di-
verse ion exchange systems, including process configurations only available
with Lewatit® product technology.
High-quality Products
Providing high-quality products is crucial for our business success.
Our global production sites are carefully controlled in order to
ensure the highest quality possible, no matter where our products
are produced.
Reliable Service
We provide a high level of technical expertise and do our best to
support you wherever we can. Our global technical sales team will
help you find the best product for your needs.
Innovative Solutions
We are continuously investing in research and development in
order to optimize our products and discover innovative uses for
our ion exchange resins, adsorbers, and iron oxide adsorbers.
SPECIFIC SOLUTIONS FOR PHARMACEUTICAL
AND BIOPROCESSING INDUSTRIES
The production of biomolecules deriving from natural required for industries such as bioprocessing and biotech-
extracts and bioprocessing fermentation processes is
nology, pharmaceutical, biopharma ceutical, nutraceutical,
strongly supported by a wide range of Lewatit® ion exchange and food.
and adsorber resins. This resin technology opens up a variety
of capture and purification options for all kinds of operation Capture and purification of biomolecules such as peptides,
scales. Our Lewatit® products are specifically designed to antibiotics, vitamins, amino acids, and polysaccharides is
be used in downstream (post-fermentation) processing of based on classical ion exchange or adsorption processes,
various biomolecules, including products from a nimal- and enabling, e.g., detoxification, conversion, decolorization, and
plant-sourced natural extracts. All resins are based on hydro- demineralization of biomolecules.
phobic (polystyrene-divinylbenzene) or m edium h ydrophilic
(polyacrylic) matrices and are specifically designed to meet A classical downstream post-fermentation approach includ-
the highest demands for downstream processing support ing resin support is shown in scheme 1.
Scheme 1: Efficient capture and purification of biomolecules is driven by Lewatit® resins and adsorbents.
Upstream process Downstream process
Upstream process Downstream process
Recovery Capture Purification Polishing and Formulation
Recovery Capture Purification Polishing and formulation
Mechanical extraction
Mechanical extraction Separation ofof
Separation Lewatit® resin
Lewatit
®
resinor
or Lewatit® resin
Lewatit
®
resinor
or FineFine purification,virus
purification, virus
ofofnatural
natural extract/
extract / biomolecule from
biomolecule from adsorbentaddition
adsorbent addition adsorbent addition
adsorbent addition deactivation,formulation
deactivation, formulation,
fermentation ororcell
fermentation cell biological tissue
biological tissue forcapture
for captureof
of for purification
for purificationofof etc.etc., to achievepharma-
to achieve pharma-
culture process
culture process biomolecule
biomolecule crude quality
crude quality ceutical, biopharma-
ceutical, biopharma-
biomolecule
biomolecule ceutical or or
ceutical, food grade
food-grade
LANXESS – Lewatit® ion exchange resins and adsorbents for capture and purification of biomolecules 3
Ion exchange resins and adsorbents are used in various Classically, resins are utilized in either the capture of bio
pharma ceutical and bioprocessing applications to sup- molecules by isolating them from complex mixtures, or the
port the manufacturing of all kinds of biomolecules via their separation of biomolecules via capture and selective e lution,
capture and purification. Table 1 gives an overview of Lewatit® or the purification of biomolecules via, e.g., demineraliza-
resins used in biomolecule production processes. tion or d
ecolorization, or the stabilization of biomolecules via
neutralization or conversion.
Table 1: Examples of pharmaceutical and biotechnological applications using Lewatit® products.
SAC WAC SBA WBA Adsorber Carrier
Lewatit® VP OC 1064 MD PH
Lewatit® MonoPlus TP 207
Lewatit® PH 1062 MDS
Lewatit® PH 1061 MDS
Lewatit® PH 1074 HEP
Lewatit® LGP 5392 PH
Lewatit® LGP 3789 FK
Lewatit® VP OC 1600
Lewatit® SC 104 PH
Lewatit® PH 2061
Lewatit® PH 8021
Lewatit® PH 7061
Lewatit® PH 5021
Lewatit® PH 3021
Lewatit® PH 1061
Lewatit® SP 120
Biomolecule
Antibiotic
Amino acid
Botanical extract
Animal extract
Vitamin
Peptide
Polysaccharide
Use of ion exchange resin and adsorbers is state-of-the-art technology
Use of ion exchange resin and adsorbers is recommended
4 LANXESS – Lewatit® ion exchange resins and adsorbents for capture and purification of biomolecules
APPLICATION EXAMPLES
Antibiotics
Antibiotics are derived from fermentation or chemical
synthesis. Lewatit® resins and adsorbents are utilized
during capture and purification, e.g., within cephalosporin,
streptomycin, gentamicin, neomycin, erythromycin, and
vancomycin production. Supported processes are various,
ranging from isolation (e.g., Lewatit® VP OC 1064 MD PH,
Lewatit® PH 8021), decolorization (e.g., Lewatit® PH 7061),
conversion (e.g., Lewatit® 1061, Lewatit® 7061), deminerali- Polysaccharides
zation, and polishing (e.g., Lewatit® PH 3021).
A large selection of marketed pharmaceuticals is based on
polysaccharides and derived either from fermentation pro-
Amino acids cesses or natural extracts. One well-known product class
originating from animal sources is GAGs (glycosylated
Amino acids are the building blocks of proteins and play a key
amino glycans), such as heparin, chondroitin, nadroparin,
role in the existence of living organisms. They are produced in
dermatan, and others. Their capture and purification is typi-
bioreactors via the bacterial fermentation of carbohydrates.
cally done with ion exchange resins (Lewatit® PH 1074 HEP)
Industrial purification of most types of amino acids often
to allow the high yield, activity, and purity of end products
involves ion exchange resins and adsorbents steps. They
(Scheme 2). An important feature of resin involves a specially
can be separated from each other using strong acidic cation
designed macroporous structure to allow the best condi-
exchange resins (e.g., Lewatit® PH 2061 MDS), captured
tions for adsorption and desorption. Lewatit® PH 1074 HEP
from a complex fermentation broth mixture by separation
is added to the digestion mixture, takes up the GAG, and
from non-ionic impurities (e.g., Lewatit® PH 1061, Lewatit®
is finally separated from solution. Afterwards, the elution of,
PH 5021) and decolorized (Lewatit® VP OC 1064 MD PH).
e.g., heparin, from ion exchange resins is done with brine.
Selection of resin types is based on the type and charge
of amino acid. Lewatit® supports the production of amino
acids, ranging from BCAAs (branched-chain amino acids)
to u nbranched ones, such as lysin, threonine, tryptophan,
histidine, arginine, serine, and many more.
Scheme 2: Purification steps of GAGs, such as heparin, by capture and elution using Lewatit® PH 1074 HEP
Concentration/
Digestion Capture Separation Elution Ultrafiltration purification
Liquid Fine purification,
Lewatit® PH
for example, by
Digestion 1074 HEP
Lewatit® PH 1074 HEP,
eluate Lewatit® PH bleaching,
alcohol precipitation
Solid 1074 HEP
UF Heparin
Optional
pH > 7 pH ~ neutral Storage of crude 1 W
ater or <3.5%
T > 50 °C 10–13 hr heparin on IER NaCl
NaOH/NaCl T variable 2 Alkaline NaCl
3–16 hr NaCl conc. >14% or gradient
Protease variable
LANXESS – Lewatit® ion exchange resins and adsorbents for capture and purification of biomolecules 5
PRODUCT PORTFOLIO
Table 2: A diversified strong portfolio of Lewatit® solutions for biomolecules production
Lewatit® Product matrix Type Group TC1 Bead size2
LGP 3789 FK Styrene/DVB, macro SAC -SO3 - H+ 1.0 HD, share 0.1 – 0.5
LGP 5392 PH Styrene/DVB, macro SAC -SO3 - H+ 1.7 HD, share 0.32 – 0.42
PH 1061 MDS Styrene/DVB, gel SAC -SO3 - K+ 1.5 (H) MD, d50 0.25 – 0.31
PH 1062 MDS Styrene/DVB, gel SAC -SO3 - Na+ 1.6 (H) MD, d50 0.25 – 0.31
PH 1061 Styrene/DVB, gel SAC -SO3 - Na+ 2.2 MD, d50 0.57 – 0.67
PH 2061 Styrene/DVB, macro SAC -SO3 - Na+ 1.7 MD, d50 0.60 – 0.70
SC 104 PH Styrene/DVB, gel SAC -SO3 - H+ 1.2 HD, d10 0.50 – 0.62
SP 120 Styrene/DVB, macro SAC -SO3 - Na+ 1.4 HD, d10 0.42 – 0.58
MonoPlus TP 207 Styrene/DVB, macro WAC -N(CH2CO2 -Na+)2 2.0 (H) MD, d50 0.56 – 0.66
PH 8021 Polyacrylate, macro WAC -CO2H 4.3 HD, d10 0.48 – 0.58
PH 1074 HEP Polyacrylate, macro SBA -N(CH ) Cl-
3 3
+
0.7 HD, d10 0.50 – 0.65
PH 7061 Styrene/DVB, macro SBA -N+(CH3)Cl- 1.0 MD, d50 0.57 – 0.67
PH 3021 Styrene/DVB, macro WBA -N(CH3)2 1.7 HD, d10 0.50 – 0.60
PH 5021 Polyacrylate, macro WBA -N(C2H4NH2)2 3.4 HD, d10 ≥ 0.4
VP OC 1064 MD PH Styrene/DVB, macro ADS None BET 800 m /g 2
MD, d50 0.44 – 0.54
VP OC 1600 Styrene/DVB, macro CAR None – HD, d10 0.32 – 0.45
Note:
[1] TC = total capacity (delivery form) in min. eq/L
[2] M
D = monodisperse with mean bead size d50. HD = heterodisperse with effective size d10 or share of >90 vol% in mm.
Please refer to technical product data sheet for complete and updated information.
OUTSTANDING FEATURES
With ion exchange resin competency since 1936, our An additional feature is provided for strong acidic cation
technical experts and global sales force can support you exchange resins, which are produced by a 1,2-dichloroethane-
concerning any process or product task that arises. Benefits free production process, thus delivering a more sustainable
of Lewatit® products include good chemical and mechani- solution.
cal bead stabilities, excellent kinetics and selectivities, high
capacities, and good economics due to lean design and high Specific products, such as Lewatit® PH 3021 and Lewatit®
performance. Our unique monodisperse production technol- MonoPlus TP 207, are produced via a unique phthalimide
ogy, offering narrow bead-size distribution, is used for a wide production chemistry, achieving full substitution control and
variety of Lewatit® ion exchange resins and adsorbents for the true weak-base resins.
capture and purification of biomolecules.
6 LANXESS – Lewatit® ion exchange resins and adsorbents for capture and purification of biomolecules
REGULATORY SUPPORT
LANXESS is a globally leading manufacturer of ion exchange,
Non-GMO
catalyst, adsorbent, and specialty resins, with global head-
TSE/BSE
quarters in Cologne, Germany. All manufacturing sites are
Allergens
routinely audited. Our in-house regulatory compliance experts
Heavy metals
for TSCA, REACH, etc., are available for immediate and the
Halal
very best support. High product quality is ensured by the strict
Kosher
quality control of each single manufactured batch before its
EU regulation No 1935/2004 of the European
release to shipment, accompanied by a certificate of analysis
Parliament and the Council of the European Union on
for each production lot. Based on customer requirements,
materials and articles intended to come into contact
exclusive sampling support is offered for the introduction of
with food
Lewatit® products into your process.
Council of Europe Resolution ResAP(2004)3 on ion
exchange and adsorbent resins used in the process-
Our biopharmaceutical-grade Lewatit® ion exchange resins
ing of foodstuffs
and adsorbents are compliant with the following certificates
EU regulation No 2023/2006 on good manufactur-
and statements:
ing practice for materials and articles intended to
come into contact with food
ISO 9001: 2015 Quality Management System
specifications
ISO 14001: 2015 Environmental Management
System requirements
Please inform technical sales person in case FDA
compliance is intended to apply.
Ion exchange resins that meet corresponding contact require-
ments with regard to their composition must be pretreated by
the user in accordance with the manufacturer’s instructions to
comply with the extraction limits described in the a ssociated
regulations.
All complete and updated information including technical product data sheets, pretreatment recommendations, certificates,
and statements are available for download on our website.
https://lanxess.com/en/Products-and-Solutions/Brands/Lewatit
Note: Lewatit® PH 5021 and Lewatit® MonoPlus TP 207 are provided with a batch-related manufacturer’s declaration
LANXESS – Lewatit® ion exchange resins and adsorbents for capture and purification of biomolecules 7
The above formulation is intended solely as guide. It and our technical advice – whether verbal, in writing or by
way of trials – is subject to change without notice and given in good faith but without warranty or guarantee,
express or implied, and this also applies where proprietary rights of third parties are involved. Our advice does
not release you from the obligation to verify the information currently provided – especially that contained in our
LANXESS Deutschland GmbH safety data and technical information sheets – and to test our products as to their suitability for the intended
Business Unit processes and uses. Further, although the ingredients, quantities thereof and properties of compounds or
finished goods mentioned herein reflect our recommendation at the time of publication, this guide may not
Liquid Purification Technologies be subject to continuous review and/or updating. The application, use and processing of our products and the
Kennedyplatz 1 products manufactured by you on the basis of our technical advice are beyond our control and, therefore, entirely
your own responsibility. Our products are sold in accordance with the current version of our General Conditions
50569 Cologne, Germany of Sale and Delivery.
Contact: Questions regarding our products and their use are welcome at any time. Please contact our sales
Phone: +49 221 8885-0 representative in your country. All contact details can be found on https://lewatit.com/
Email: [email protected]
Edition: January 2023
Lewatit®, LewaPlus®, and Bayoxide® are registered trademarks of LANXESS Deutschland GmbH.
11232 EN
Images: Adobe Stock (page 4, 5, 7), LANXESS (page 1, 2, 3)
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