MRS Agar, Modified M1990I
Intended Use:
Recommended isolation and enumeration of mesophilic lactic acid bacteria from food. The composition and performance
criteria are in accordance with in ISO 15214:1998.
Composition**
Ingredients Gms / Litre
Tryptone $ 10.000
HM extract ⊖ 10.000
Yeast extract 4.000
Triammonium citrate 2.000
Sodium acetate 5.000
Magnesium sulphate.7H2O 0.200
Manganese sulfate. 4H2O 0.050
Dipotassium hydrogen phosphate 2.000
Dextrose (Glucose) 20.000
Polysorbate 80 (Tween 80) 1.080
Agar 15.000
Final pH ( at 25°C) 5.7±0.1
**Formula adjusted, standardized to suit performance parameters.
$ -Equivalent to Enzymatic digest of casein
⊖ -Equivalent to Meat extract
Directions
Suspend 69.21 grams (equivalent weight of dehydrated medium per litre) in 1000 ml purified / distilled water.
Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri
plates.
Principle And Interpretation
MRS Agar, Modified is in accordance with ISO (1),for the enumeration of mesophilic lactic acid bacteria. Mesophilic bacteria
are divided into two groups: Lactic Acid Starter bacteria (including Lactococcus lactis subsp. lactis and Lactococcus lactis
subsp. cremoris ), which are primarily used for producing lactic acid, and Aroma Producing bacteria (including Lactococcus
lactis subsp. lactis biovar diacetylactis and Leuconostoc mesenteriodes subsp. cremoris), which are primarily used for
producing CO2 gas and flavor. MRS Agar, Modified (Lactobacillus Heteroferm Screen Agar)
recommended by APHA (3), is used for the isolation and cultivation of Lactobacillus species from salad dressings (5).
Tryptone, HM extract and yeast extract supply nitrogen, carbon and other elements essential for the growth of Lactobacilli.
Dextrose (Glucose) is the carbohydrate source. Polysorbate 80 (Tween 80) supplies fatty acids required by Lactobacilli.
Triammonium citrate, sodium acetate inhibit gram-negative organisms and certain gram-positive bacteria.
Type of specimen
Food and animal feeding stuff
Specimen Collection and Handling:
For food and animal feeding stuff samples, follow appropriate techniques for sample collection and processing as per
guidelines (4).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection.
Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per
established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual
safety data sheets.
Limitations
Please refer disclaimer Overleaf.
HiMedia Laboratories Technical Data
1. Due to nutritional variation some species of lactobacilli may not show growth.
2. This medium is general purpose medium and may not support the growth of fastidious
organisms.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at
recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Yellow coloured slight opalescent gel forms in Petri plates
Reaction
Reaction of 6.92% w/v aqueous solution at 25°C. pH : 5.7±0.1
pH
5.60-5.80
Cultural Response
Cultural characteristics observed after an incubation at 30°C for 18-48 hours with 5% CO2.
Organism Inoculum Growth Recovery
(CFU)
Lactobacillus sakei ATCC 50-100 good-luxuriant >=50%
15521 (00015*)
Lactococcus lactis ATCC 50-100 good-luxuriant >=50%
19435 (00016*)
Escherichia coli ATCC >=104 inhibited 0%
8739 (00012*)
Key : (*) Corresponding WDCM numbers.
Storage and Shelf Life
Store dehydrated and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should
be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic
nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area
protected from extremes of temperature and sources of ignition Seal the container tightly after use.
Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow
established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical
sample must be decontaminated and disposed of in accordance with current laboratory techniques (1,2).
Reference
1. Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.
2. Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)
Manual of Clinical Microbiology, 11th Edition. Vol. 1.
3. Microbiology of food and animal feeding stuffs-Horizontal method for the enumeration of mesophilic lactic acid
bacteria. ISO 15214:1998(E).
4. Salfinger Y., and Tortorello M.L. Fifth (Ed.), 2015, Compendium of Methods for the Microbiological Examination of
Foods, 5th Ed., American Public Health Association, Washington, D.C.
5. Smittle R. B. and Flowers R. M., 1982, J. Food Protection, 45:977.
Revision : 01 / 2019
Disclaimer :
User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in
this and other related HiMedia™ publications. The information contained in this publication is based on our research and development
work and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes to
specifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use but
for laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not
be considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.
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