Appendix 3.

Conversion factors to be applied in the continuity equations for COD, N, P, Mg, K, Ca, mass, carbon and proton of the model.

Conservation for COD N P Mg K Ca Mass Carbon Proton

ICODi INi IPi IMgi IKi iCai ITSSi iCi iHi
Component Units g COD gN gP g Mg gK g Ca g TSS mol C mol H
SO2 g O2 -1
SF g COD 1 iNSF iPSF iMgSF iKSF iCaSF iCSF
SA g COD 1 2/64 1/64
SNH4 gN 1 -1/14
SNO3 gN -64/24 1 1/14
SPO4 gP 1 2/31
SI g COD 1 iNSI iPSI iMgSI iKSI iCaSI iCSI
SN2 gN -24/14 1
XI g COD 1 iNXI iPXI iMgXI iKXI iCaXI iTSSXI iCXI
XS g COD 1 iNXS iPXS iMgXS iKXS iCaXS iTSSXS iCXS
XH g COD 1 iNBM iPBM iMgBM iKBM iCaBM iTSSBM 5/160
XPAO g COD 1 iNBM iPBM iMgBM iKBM iCaBM iTSSBM 5/160
XPHA g COD 1 0.6 1/36
XPP gP 1 iMgPP* iKPP** 3.23
XAUT g COD 1 iNBM iPBM iMgBM iKBM iCaBM iTSSBM 5/160
SC mol C 1 2
+
SH mol H -1
SK gK 1
SMg g Mg 1
SCa g Ca 1
XACP mol ACP 31∙2 40.1∙3 364.2
XHAP mol HAP 31∙3 40.1∙5 502.3
XTSS g TSS -1

*iMgPP = 0.28 gMg/gP (Barat et al., 2005)

**iKPP = 0.35 gMg/gP (Barat et al., 2005)
Appendix 4. Kinetic expressions of ASM2d (Henze et al., 1999) extended with pH model (Serralta et al.,
2004), K and Mg model (Barat et al. 2005) and precipitation model (this study).

Process Kinetic expression

1. Aerobic
hydrolysis

2. Anoxic hydrolysis

3. Anaerobic
hydrolysis

4. Aerobic growth
on SF
5. Aerobic growth
on SA
6. Anoxic growth
on SF
7. Anoxic growth
on SA
8. Lysis XH

9. Storage of PHA

10. Aerobic storage

of poly-P

11. Anoxic storage

of poly-P

12. Aerobic growth

on XPHA

13. Anoxic growth

on XPHA

14. Lysis de XPAO

15. Lysis de XPP
16. Lysis de XPHA
17. Aerobic growth
(Nitrification)
18. Lysis XA
19. CO2 stripping

20. ACP
precipitation

21. ACP dissolution

22. HAP
precipitation
Being:

SH represents free proton concentration, KpH and KI,pH the half-saturation and inhibition parameters for pH,
and fpH the maximum value of the product of both switching functions. This factor has been used to remove
pH inhibition at optimal pH conditions (IpH = 1 for SH = (KpH·KI,pH)1/2).
Appendix 5. Kinetic coefficients of ASM2d (Henze et al., 1999) extended with pH model (Serralta et al.,
2004), K and Mg model (Barat et al. 2005) and precipitation model (this study).

Kinetic Description Value Units Source

coefficient
Hydrolysis of particulate substrate
-1
Hydrolysis rate constant (20 ºC) 3.00 d Henze et al. (1999)
Anoxic hydrolysis reduction factor (20 ºC) 0.60 --- Henze et al. (1999)
Anaerobic hydrolysis reduction factor (20 ºC) 0.40 --- Henze et al. (1999)
-3
Saturation/inhibition coefficient for oxygen (20 ºC) 0.20 g O2 m Henze et al. (1999)
-3
Saturation/inhibition coefficient for nitrate (20 ºC) 0.50 gNm Henze et al. (1999)
-1
Saturation coefficient for particulate COD 0.10 g XS g XH Henze et al. (1999)
Heterotrophic organisms
-1
Maximum growth rate on substrate (20 ºC) 6.00 d Henze et al. (1999)
-3
Saturation/inhibition coefficient for oxygen (20 ºC) 0.20 g O2 m Henze et al. (1999)
-3
Saturation coefficient for growth on SF (20 ºC) 4.00 g COD m Henze et al. (1999)
-3
Saturation coefficient for phosphate (nutrient) (20 ºC) 0.01 gPm Henze et al. (1999)
-3
Saturation coefficient for ammonium (nutrient) (20 ºC) 0.05 gNm Henze et al. (1999)
-3
Saturation coefficient for growth on SA (20 ºC) 4.00 g COD m Henze et al. (1999)
Anoxic growth reduction factor (20 ºC) 0.80 --- Henze et al. (1999)
-1
Rate constant for lysis and decay (20 ºC) 0.40 d Henze et al. (1999)
-7 + 3
Saturation coefficient for pH 2.3∙10 mol H /m Serralta et al. (2004)
-3 + 3
Inhibition coefficient for pH 4.35∙10 mol H /m Serralta et al. (2004)
-3
Saturation coefficient for nitrate (20 ºC) 0.50 gNm Henze et al. (1999)
Phosphorus accumulating organisms (PAO)
-1 -1
Rate constant for storage of PHA (20 ºC) 4.00 g XPHA g XPAO d This study
-3
Saturation coefficient for acetate (20 ºC) 4.00 g COD m Henze et al. (1999)
-1
Saturation coefficient for polyphosphate (20 ºC) 0.01 g XPP g XPAO Henze et al. (1999)
-1 -1
Rate constant for storage of PP (20 ºC) 1.50 g XPP g XPAO d Henze et al. (1999)
-3
Saturation/inhibition coefficient for oxygen (20 ºC) 0.20 g O2 m Henze et al. (1999)
-3
Saturation coefficient for phosphate (nutrient) (20 ºC) 0.01 gPm Henze et al. (1999)
-3
Saturation coefficient for phosphate in storage PP (20 ºC) 0.20 gPm Henze et al. (1999)
-1
Saturation coefficient for PHA in storage PP (20 ºC) 0.01 g XPHA g XPAO Henze et al. (1999)
-1
Maximum ratio of XPP/XPAO 0.34 g XPP g XPAO Henze et al. (1999)
-1
Inhibition coefficient for PP storage 0.02 g XPP g XPAO Henze et al. (1999)
-3
Saturation coefficient for nitrate (20 ºC) 0.50 gNm Henze et al. (1999)
-3
Saturation coefficient for magnesium (20 ºC) 5.00 g Mg m Barat et al. (2005)
-3
Saturation coefficient for potassium (20 ºC) 3.00 gKm Barat et al. (2005)
-1
Maximum growth rate of PAO (20 ºC) 1.00 d Henze et al. (1999)
-3
Saturation coefficient for ammonium (nutrient) (20 ºC) 0.05 gNm Henze et al. (1999)
-1
Saturation coefficient for PHA in growth of PAO (20 ºC) 0.01 g XPHA g XPAO Henze et al. (1999)
Anoxic growth reduction factor (20 ºC) 0.60 --- Henze et al. (1999)
-1
Rate constant for lysis of PAO (20 ºC) 0.20 d Henze et al. (1999)
-1
Rate constant for lysis of PP (20 ºC) 0.20 d Henze et al. (1999)
-1
Rate constant for lysis of PHA (20 ºC) 0.20 d Henze et al. (1999)
-6 + 3
Saturation coefficient for pH 2.28·10 mol H /m Serralta et al. (2004)
-4 + 3
Inhibition coefficient for pH 6.94·10 mol H /m Serralta et al. (2004)
Autotrophic organisms
-1
Maximum growth rate (20 ºC) 1.00 d Henze et al. (1999)
-1
Rate constant for lysis (20 ºC) 0.15 d Henze et al. (1999)
-3
Saturation coefficient for oxygen (20 ºC) 0.50 g O2 m Henze et al. (1999)
-3
Saturation coefficient for ammonium (substrate) (20 ºC) 1.00 gNm Henze et al. (1999)
-3
Saturation coefficient for phosphate (nutrient) (20 ºC) 0.01 gPm Henze et al. (1999)
Precipitation
-26.0 -1 5
ACP solubility product 10 (mol l ) This study
+8 -1 -1
ACP precipitation constant rate 6.010 l mol d This study
-9 -1 -3
ACP precipitation hyperbolic inhibition constant 0.610 Mg m This study
+8 -1 -1
ACP dissolution constant rate 2.010 l mol d This study
-3 -1
ACP dissolution hyperbolic inhibition constant 0.210 mol l This study
-2 -1 -1
HAP precipitation constant rate 0.210 mol l d This study
-3 -1
HAP precipitation hyperbolic inhibition constant 0.510 mol l This study
Appendix 6. Stoichiometric parameters of ASM2d (Henze et al., 1999) extended with pH model (Serralta et
al., 2004), K and Mg model (Barat et al. 2005) and precipitation model (this study).

Kinetic Description Value Units Source

coefficient
Hydrolysis of particulate substrate
-1
fSI Production of SI in hydrolysis 0 g COD g COD Henze et al. 1999
Heterotrophic organisms
-1
YH Yield coefficient 0.625 g COD g COD Henze et al. 1999
-1
fXI Fraction of inert COD generated in biomass 0.10 g COD g COD Henze et al. 1999
lysis
Phosphorus accumulating organisms (PAO)
-1
YPAO Yield coefficient under aerobic conditions 0.625 g COD g COD Henze et al. 1999
-1
YPAO,NO Yield coefficient under anoxic conditions 0.500 g COD g COD Garci-Usach et al. 2010
-1
YPHA PHA requirement for PP storage under 0.320 g COD g P This study
aerobic conditions
-1
YPHA,NO PHA requirement for PP storage under 0.590 g COD g P Garci-Usach et al. 2010
anoxic conditions
-1
YPO4 PO4 released per PHA stored 0.52-0.52-0.65-0.74 g P g COD This study
-1
fXI Fraction of inert COD generated in biomass 0.10 g COD g COD Henze et al. 1999
lysis
Autotrophic organisms
-1
YA Yield of autotrophic biomass 0.24 g COD g N Henze et al. 1999
-1
fXI Fraction of inert COD generated in biomass 0.10 g COD g COD Henze et al. 1999
lysis