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Standard Graph

The document contains information about potassium, phosphorus, and unknown standards including: 1) Absorbance measurements for potassium standards at concentrations of 0, 5, 10, 15, and 20 mg/ml with the linear regression equation and R2 value. 2) Absorbance measurements for phosphorus standards at concentrations of 1, 2, 4, 6, and 10 μg/ml with the linear regression equation and R2 value. 3) Absorbance measurements for an unknown standard at concentrations of 5, 10, 15, and 20 with the linear regression equation, intercept, slope, and calculation to determine the unknown concentration using the regression equation.

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VAISHNAVI JADHAV
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35 views7 pages

Standard Graph

The document contains information about potassium, phosphorus, and unknown standards including: 1) Absorbance measurements for potassium standards at concentrations of 0, 5, 10, 15, and 20 mg/ml with the linear regression equation and R2 value. 2) Absorbance measurements for phosphorus standards at concentrations of 1, 2, 4, 6, and 10 μg/ml with the linear regression equation and R2 value. 3) Absorbance measurements for an unknown standard at concentrations of 5, 10, 15, and 20 with the linear regression equation, intercept, slope, and calculation to determine the unknown concentration using the regression equation.

Uploaded by

VAISHNAVI JADHAV
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as XLSX, PDF, TXT or read online on Scribd

potassium standards

Potassium estimation
STANDARD (mg/ml) ABSORBANCE 2
0 0 1.8
1.6 f(x) = 0.085 x + 0.022
5 0.56 R² = 0.98321793280641
1.4
10 0.78

aBSORBANCE
1.2
15 1.23 1
20 1.79 0.8
0.6
0.4
0.2
0
0 5 10 15
slope 0.085 St. mg/l
inter 0.022

x=(y+ 0.022)/0.085
6.847059
Potassium estimation

x + 0.022
1793280641

5 10 15 20 25
St. mg/l
x=(y+intercept)/slope
phosphorus standards

standard (µg/ml) absorbance


1 0.075
2 0.081
4 0.115 absorbance V
6 0.156
0.25
10 0.203
0.2 f(x) = 0.01494140625 x +

absorbance at 660nm
R² = 0.987402883660159
0.15
slope 0.01494140625
intercept 0.05726953125 0.1

0.05

0
0 2 4
concentration
absorbance Vs conc (µg/ml)

f(x) = 0.01494140625 x + 0.05726953125


R² = 0.987402883660159

0 2 4 6 8 10 12
concentration of phosphorus (µg/ml)
std. absorbance
5 0.56 absorbance
10 0.75
1
15 0.85 0.9 f(x) = 0.0254 x + 0.46
20 0.95 R² = 0.970749322900993
0.8
0.7
0.6
0.5

Abs
0.4
0.3
0.2
0.1
0
4 6 8 10 12 14 16
Std.

slope 0.0254
intern 0.46

x=(Y+ intercept)/Slope
29.92126
sorbance

12 14 16 18 20 22
Std.

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