ANSWERS
1. When turning a high-power objective to a place, it is essential to make sure that the objective is firmly
attached and that the eyepiece is correctly aligned. The image will be correctly focused, and the field of
vision will be increased as a result. It is also important to ensure that the goal is appropriately lighted and
that the light source is of enough brightness. Finally, ensure that the objective is clean and clear of dust and
dirt, as this might influence the image's quality.
2. Always remember to use the coarse adjustment only while focusing the instrument with the low power
objective lens. When using the coarse adjustment knob with higher power objective lenses, the lens may
collide with the slide, potentially destroying it. When adjusting upward, it is crucial to start with the lowest
power objective lens.
3. The diaphragm, also known as the iris diaphragm, is important in microscopy because it controls the
quantity of light that goes through the specimen. It is changed in various situations to meet distinctive
microscopy needs. To begin with, it is used to adjust to changing lighting conditions by expanding or
narrowing the diaphragm, allowing for exact control of light intensity. Second, by changing the diaphragm,
researchers might enhance specimen contrast, allowing minute features and structures to be seen more
clearly. Furthermore, to get excellent picture quality and clarity, the diaphragm must be adjusted to obtain
the ideal light level for the specimen. While working with specimens of varied depths, the diaphragm helps
to get the proper depth of field. It also helps to reduce glare and reflections.
4. In handling the microscope slide, always remember that do not touch the microscope's objective lens with
your fingers instead maintain the lenses clean by wiping them with lens tissue. Always begin viewing a
slide with the use of the microscope's lowest magnification. Always keep your microscope wrapped when
not in use. Always carry a microscope with the use of both hands and hold it by the edges part and keep in
mind to slide the microscope slides at the end and never view it without the coverslip
5. The purpose of the stage clips on a microscope is to keep the specimen slide in place on the microscope's
stage, ensuring stability and exact positioning for accurate observation and analysis. It keeps the material
from moving unintentionally, allowing for consistent and safe examination under the microscope.
6. You should open both eyes when looking through the microscope. This reduces eye drowsiness caused by
keeping the nonviewing eye closed. It takes some work to keep both eyes open, but it is strongly advised.
Also, never let your eye touch the ocular lens, and if you wear glasses, never let it touch the lens.
7. It is important to understand how to handle a microscope. Handle it with two hands Handling the
microscope with two hands, one on the arm and the other on the base, provides a stable way for carrying the
microscope, preventing it from falling and perhaps harming it.
8. In a microscope, the coverslip provides the essential task of flattening and protecting the specimen put on a
microscope slide. It reduces air gaps and distortion by gently dropping a thin, transparent coverslip onto the
specimen using a mounting media (typically a liquid), allowing for a better and more thorough view under
the microscope. Furthermore, the coverslip protects the specimen from external contamination and keeps it
from drying up, assuring the sample's longevity throughout examination.
9. The objective lens used to locate the specimen and first focus is typically the course adjustment and it is the
lowest power objective on a microscope. This objective lens is usually labeled with a magnification factor
of around lower power objective around 4x or 10x.
10. Methylene Blue is a chemical that is occasionally used to make the specimen visible. It is a common
alkaline stain for seeing tiny life in vivid color. It aids in making cells stand out against their background,
where their form can aid in determining what they are.
11. If the high-power objective lens touches or breaks the coverslip, stop immediately and ask assistance to the
teacher or instructor.
DOCUMENTATIONS
