TCC2 Electronics Module
Time-Resolved Photon Counting
Features
• TSCPC time range: 2.5 ns - 50 ms
• MCS time range: 5 ms - 200 s
• Time resolution from 305 fs/ch
• Rep rates up to 100 MHz
• Forward and reverse modes
• Up to 3 detector channels
Applications
The TCC2 is a unique electronics module for the measurement of kinetics via Time-
Correlated Single Photon Counting (TCSPC) and Multi-Channel Scaling (MCS), • Time-Resolved Fluorescence (TCSPC)
and allows three sources and three detectors to be permanently connected.
• Phosphorescence Lifetime (MCS)
In TCSPC mode, kinetics from a few picoseconds can be measured using a high
• Förster Resonance Energy Transfer (FRET)
repetition rate source. TCSPC modules are generally available in two forms: with
an Analogue-to-Digital Convertor which can have high temporal resolution, but • Optical Tomography
may suffer from lower acquisition speed; or, with a Time-to-Digital Convertor • Time of Flight / LIDAR
(TDC) which has higher acuisition speed but at the expense of a reduced
temporal resolution. The Edinburgh Instruments TCC2 works with an ultra-fast
flash Analogue-to-Digital convertor and proprietary dead-time management.
This demonstrates unrivalled temporal performance with acquisition frequencies
up to 100 MHz. The module also incorporates novel electronics that eliminate
the need for cable swapping or external delay selection.
In MCS mode, kinetics from ~10 ns up to seconds can be measured with
frequencies up to 1 MHz. The built-in TCSPC and MCS operations complement
each other to allow the fast, simple measurement of kinetics over twelve orders
of magnitude. The TCC2 is supplied with full data acquisition and lifetime
analysis software.
Time-Resolved Emission Spectroscopy (TRES)
TRES is a powerful tool in fluorescence lifetime studies. This measurement example shows complex fluorescence decay kinetics for a single fluorophore;
Norharman, dissolved in a protic solvent. The TCC2 data acquisition electronics work seamlessly with all of the comprehensive fluorescence data
analysis software packages available from Edinburgh Instruments.
104
103
Counts
102
10
40
1 20 4
0 60 30
0 20 40 60 80 100 80 1 20
Time 00 12
0 140 1
0
Time (ns) 0 20 40 60 80 100 120 140 160
(ns)
Time (ns)
Sample: Norharman in methanol (5×10-5M)
Excitation Source: 280 nm pulsed LED (EPLED-280)
Results: The resulting TRES map details the three different exponential lifetimes with overlapping emission bands of Norharman, highlighting the three different
forms present; neutral = 2.3 ns, zwitterion = 5.1 ns, and cation = 18.0 ns
Technical Specifications
Start & Stop Inputs Combining TCSPC and MCS data acquisition in one electronics
Type Constant Fraction Delay module enables a lifetime range that extends over twelve orders
Input Pulse Height 0 to – 3000 mV
of magnitude, from a few picoseconds to seconds.
Threshold 0 to – 500 mV
Constant Fraction 0.3, fixed
Constant Fraction Delay Plug-in Delay
Frequency Divider 1, 2, 4, 8
Input Connectors SMA, 50 Ω
Jitter 20 ps
The graph illustrates the range of kinetics that can be measured
Data Acquisition
by both data acquisition techniques.
Modes of Operation Forward or Reverse
Shifting Delay 0 to 800 ns in steps 104
Number of Detector
3, computer controlled
Channels
103
Number of Synchronisation
Counts
3, computer controlled
Channels
Number of Reference 102
1
Channels
Number of Trigger 10
1, to trigger MCS operation
Channels
TCSPC Operation 1
0 1000 2000 3000 4000
Time Range 2.5 ns to 50 ms Time (ps)
Residuals
3.7
Number of Channels 512, 1024, 2048, 4096, 8192
0
Minimum Time per Channel 305 fs
-3.7
Max Repetition Rate Up to 100 MHz
MCS Operation Sample: DASPI in ethanol
Time Range 5 ms to 200 s Excitation Source: 375 nm pulsed diode laser (EPL-375)
Number of Channels 500, 1000, 2000, 4000, 8000
Minimum Time per Channel 10 ns 104
Max Repetition Rate Up to 1 MHz
103
Counts
Trigger Input 0 to 5000 mV
102
Computer Interface
Interface USB 2.0
10
Dimensions 230 mm (W) x 445 mm (D) x 100 mm (H)
Power Requirements 36 V (100 - 240 V power supply included) 1
0 5 10 15 20 25
Residuals
Time (ms) c2 = 1.059
Software 7.7
Applications F980 and Fluoracle 0
-7.7
Operating System Windows 7, 8, 10
Sample: Rare-earth doped glass
Excitation Source: 60 W Microsecond flash lamp
Customer support is available worldwide
For more information contact us below or visit www.edinst.com
Edinburgh Instruments
2 Bain Square, T: +44 (0)1506 425 300 E: [email protected]
Kirkton Campus, F: +44 (0)1506 425 320
Livingston,
EH54 7DQ
United Kingdom
For our US office, contact:
T: +1-800-323-6115 E: [email protected]
F: +44 (0)1506 425 320
All specifications are correct at the time of production. We reserve the right to change our specifications without notice.
© Edinburgh Instruments Ltd. 2021
