Bg of Study
Cockroaches have been known to play a role as carriers of intestinal diseases,
such as dysentery, diarrhea, cholera, and typhoid fever. Cockroach bites can cause skin
irritation, infection, and swelling. You can commonly find cockroach in wall cracks,
behind or under furniture, below the sink, etc. Due to the problem of cockroach, the
researchers decided to search for an effective but environment-friendly killer. The
solution the researchers thought of are lemon peels because based on the study of
Lang (2019), lemon peels contain d-limonene. (5 sentences)
(Additional) The research of Karr et al., (1988) also used d limone as insecticidal
activity for German Cockroaches. Based on the study of Ibrahim et al. (2008), indicated
that bioactive limonene, in addition to its use as an insecticide to control ectoparasites,
also has activity against many insects. (2 sentences)
Signi of Study
Department of Agriculture (DA)
Cockroaches may be difficult to control without a pest control professional. But
with our study, it could help to get rid of cockroaches.
Department of Education (DepEd)
This study can be a basis for future learning materials in which students,
teachers, and DepEd officers can benefit.
Future researchers
This study benefited the student researchers in various ways. Such as, our study
can be used as their basis or guide on their product.
(Additional)
The local government
The government may use our study to prevent cockroaches from giving us
natural sickness like itching, allergic reactions, redness bumps and etc.
State of the Problem
Can Citrus limon (Lemon) peel extract be an effective insecticide against
Blattodea (Cockroach)?
(Alt)
Can Citrus limon (Lemon) peel extract be an insecticide against Baptica dubia
(Dubia roach)
Hypo
Citrus limon (Lemon) peel extract can be an effective insecticide against
Blattodea (Cockroach).
(Alt)
Citrus limon (Lemon) peel extract can be an insecticide against Baptica dubia
(Dubia roach).
Concept Frame
The study focuses on using d-limonene from Citrus limon (lemon) peel
extract in the mortality cockroaches.
Independent Variable Dependent Variable
Citrus limon (Lemon) Mortality rate of
peels cockroach
(d-limonene)
Figure 1. Framework of the Study
Scope and limit
What study aims
Where lemons bought
Where ipis bought
How it was obtained
Where?
Duration
Hours observation
Amount of d limonene
DV
Materials
500 g lemon peels
1000 mL distilled water
(2) 30 mL Spray Bottle
Stirring rod
(3) 700L Jars with cap 15 cm diameter
Eye protection
Lab Coats
Grater
Bunsen Burner
Heat resistant mat
Tripod
Gauze
Measuring Cylinder
Distillation apparatus
Weighing Scale
Egg Tray
2.2 Procedures in Making the Product
1. Prepared all the materials.
2. Lemons were washed with tap water
3. Grated the lemons, use the 250 g of lemon peels and add to 500 mL of distilled
water in the 250 mL round bottomed flask.
4. Set up the distillation
5. Heat the flask so that distillation
6. Collect approximately 15 mL of distillate
7. Carefully pour 5 mL of the distillate to spray bottle 1 and pour 10 mL of the
distillate to spray bottle 2
8. Repeat the experiment 2 times
2.3 Procedures in Testing the Product
1. Gather 9 adult cockroach and separate them in three containers
2. Name the three containers container 1, container 2, and container 4
3. Add equal amount of egg trays in each container
4. In container 1, shake then spray about 5mL of the solution directly to the
cockroaches
5. In container 2, shake then spray about 10mL of the solution directly to the
cockroaches
6. In container 4, do not spray anything.
7. After 24 hours of application, the mortality rate would be computed by the formula
mortality rate = the number of dead cockroaches/overall population.
Present of Data
Time Treatment
5 mL of d-limonene 10 mL of d-limonene Controlled Variable
1 minute after All 3 cockroaches are All 3 cockroaches are All 3 cockroaches are
spraying alive. alive. alive.
20 minutes All 3 cockroaches are All 3 cockroaches are All 3 cockroaches are
after spraying alive. alive. alive.
1 hour after All 3 cockroaches are All 3 cockroaches are All 3 cockroaches are
spraying alive. alive. alive.
2 hours after All 3 cockroaches are All 3 cockroaches are All 3 cockroaches are
spraying alive. alive. alive.
3 hours after All 3 cockroaches are All 3 cockroaches are All 3 cockroaches are
spraying alive. alive. alive.
4 hours after All 3 cockroaches are All 3 cockroaches are All 3 cockroaches are
spraying alive. alive. alive.
5 hours after All 3 cockroaches are All 3 cockroaches are All 3 cockroaches are
spraying alive. alive. alive.
12 hours after All 3 cockroaches are All 3 cockroaches are All 3 cockroaches are
spraying alive. alive. alive.
Analysis of Data
In each treatment, the testing was conducted with two different amounts of the
d-Limonene extract. With the results gathered, it was found that both of the different
amounts of the d-limonene were not effective against blattodea. After 12 hours of the
spraying process, the blattodea wasn't moving as pesky as it should be, but it was still
alive. The blattodea was still weak after 10 hours of the spraying process but again,
they were still alive. Based on the related studies, we had this kind of result due to the
lack of d-limonene.
Conclusions
After 12 hours of observing, no cockroaches died, the researchers can
conclude that Citrus limon (Lemon) peels cannot be an effective insecticide against
Blattodea (Cockroaches). After directly spraying 5 mL and 10 mL of d-limonene and
observing the cockroaches for 24 hours, there were 0 deaths. The d-limonene caused
the cockroaches to have slow mobility. The mortality rate of the cockroaches is 0%.
Therefore the researchers conclude that Citrus Limon (lemon) peels cannot be an
effective insecticide againt Blattodea (cockroaches), rejecting the hypothesis.
Recommendations
1. The researchers recommend to use cheaper citrus fruits with high amount of d-
limonene to be more effective.
2. Smaller pests are suggested by the researchers.
7. The researchers recommend soaking the peels for at least 2 – 3 minutes to
soften and is much easier to grate.
