LORMA COLLEGES

COLLEGE OF MEDICAL LABORATORY SCIENCE

City of San Fernando, 2500 La Union,
Philippines

MIDTERM EXAMINATION
1st SEM, 2022 – 2023

CLINICAL BACTERIOLOGY LABORATORY

Name of Instructor (s):

Ms. Danelene Estacio

Ms. Wyndel Kaye Catli
Ms. Dawn Kimberly Padua

Date of Exam: November 24, 2021 (Thursday)

Time: 2:00 PM-4:00 PM

TYPE OF TESTS:
I. Multiple Choice 20
II. True or False 10
III. Headings 10
IV. Matching Type 30
V. Modified Sequencing 10
VI. Analysis 20
VII. Evaluation 15
Total: 115

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 LORMA COLLEGES
COLLEGE OF MEDICAL LABORATORY SCIENCE
City of San Fernando, 2500 La Union, Philippines

CLINICAL BACTERIOLOGY LABORATORY MIDTERM EXAMINATION

I. MULTIPLE CHOICE: Choose the letter of the correct answer.

1. Most pathogens grow best at 30-37°C, these organism are referred to as:

A. Psychrophiles
B. Thermophiles
C. Mesophiles
D. Microaerophiles

2. Sheep Blood Agar is an example what type of culture media?

1. Differential 2. Selective 3. Enrichment 4. Nutritive

A. 1 and 2
B. 3 and 4
C. 1 and 4
D. 2 and 3

3. Which of the following statements is true about a clear broth culture?

A. This can be readily used to inoculate a sample into.

B. This broth culture is completely free of any possible contaminant.
C. The culture media should not be used.
D. Additional step of sterilization should still be performed.

4. Which of the following components serves as the catalyst within an anaerobic

jar?

A. Magnesium
B. Palladium
C. Methylene Blue
D. Resazurin

5. Which of the following is INCORRECTLY matched?

A. Terminal spore: Clostridium tetani

B. Central spore: Bacillus subtilis
C. Subterminal spore: Clostridium botulinum
D. Central Spore: Bacillus anthracis

6. All of the following organisms are obligate aerobes, EXCEPT for:

A. Francisella
B. Moraxellaceae
C. Vibrionaceae
D. Propionibacterium

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7. When cleansing the skin with alcohol and then iodine for the collection of a blood
for culture, the iodine should remain in contact on the skin for at least:

A. 10 seconds
B. 30 seconds
C. 60 seconds
D. 90 seconds

8. What phase of bacterial growth are organism the most susceptible to antibiotics?

A. Lag
B. Decline
C. Logarithmic
D. Stationary

9. In the preparation of a nutrient agar, when 250 milliliters of distilled water was
prepared how many grams of agar would be needed? **Manufacturer instructions:
27.45 g for every 1000 mL of water.

A. 9.15
B. 13.73
C. 36.43
D. 44.31

10. Anaerobic incubation technique requires which of the following gas mixtures?

A. 80-90% hydrogen, 5-10% oxygen, 5-10% nitrogen

B. 80-90% nitrogen, 5-10% oxygen, 5-10% hydrogen
C. 5-10% hydrogen, 80-90% carbon dioxide, 5-10% nitrogen
D. 5-10% hydrogen, 80-90% nitrogen, 5-10% carbon dioxide

11. Which of the details should NOT be included in blood culture bottle labels?

A. Patient’s name
B. Attending physician
C. Collection Site
D. Date of collection

12. Heterotrophs are organisms that obtain their carbon dioxide requirement from
inorganic matter. Heterotrophs are also called litotrophs.

A. First statement is true, second statement is false

B. First statement is false, second statement is true
C. Both statements are true
D. Both statements are false

13. What is the reaction of the color indicator of the GasPak system in anaerobic
conditions?

A. Blue to red
B. Blue to colorless
C. Colorless to blue
D. Green to blue

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14. Why is it important to store agar plates in an upside down (agar side up)
manner?

A. In order to prevent condensation from forming within the agar plate

B. For easier access and to prevent the lid from falling off.
C. To prevent possible contamination from the moisture formed.
D. All of the above

15. Which of the following statements is TRUE about the evaluation of bacterial
colonies?

A. Odor of the organism can be noted by gently whiffing off the culture media.
B. Colony size is an accurate characteristic that can be used to identify a specific
organism
C. Colony pigmentation can be enhanced by incubating the media at 37deg Celsius
D. Transillumination can be used to properly distinguish the haemolytic pattern of an
organism in BAP.

16. Which of the following media is/are enrichment media?

1. Selenite 2. Alkaline Peptone Water 3. Tetrathionate Broth 4. Blood Agar

A. 1 and 2
B. 2 and 3
C. 1, 2 and 3
D. 1, 2 3 and 4

17. Which of the following does NOT match correctly?

A. Babes-Ernst Bodies: Corynebacterium

B. Muchs Granules: Mycobacterium
C. Sulfur granules: Yersinia
D. Bipolar Bodies:Pasteurella

18. Continuous monitoring systems for blood cultures determine the presence of an
organism based on the production of:

A. Oxygen
B. Carbon
C. Carbon dioxide
D. Hydrogen peroxide

19. When a 750 mL of culture media is prepared for a blood agar, how much
millilitres of blood should be added to ensure enough nourishment?

A. 7.5
B. 37.5
C. 75
D. 375

20. The main purpose of negative staining is to study the morphological

characteristics of organism that are difficult to stain. It can also be used to stain
cells that are too delicate to be heat-fixed.

A. First statement is true, second statement is false

B. First statement is false, second statement is true
C. Both statements are true
D. Both statements are false

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 II. TRUE/FALSE: Answer TRUE if the statement is true, answer FALSE if the
statement is false.

FALSE 21. The culture medium should be dispensed into the plates at 22°C-25°C to avoid
the formation of condensation within the lids of the Petri dishes.
FALSE 22. Negative stains impart color on both the organism and the background.
FALSE 23. Crystal violet is a dye commonly used to inhibit the growth of gram negative
organism.
TRUE 24. In order to maintain a semi-solid consistency, only 1% solidifying agent should
be contained within the medium.
TRUE 25. Two to three sets of blood samples per are drawn from two independent
venipuncture sites on an adult patient in order to increase the likelihood of
isolating the pathogen.
FALSE 26. Haemophilus species require the addition of Factor X and Factor V for their
growth, therefore they are considered as non-fastidious organism.
FALSE 27. After the preparation of culture media, they should be sealed in a container or
a plastic cover and stored in the incubator.
TRUE 28. Ryu stain is one of the most commonly used flagellar staining method.
FALSE 29. All pathogenic organism can grow in an artificial growth media.
TRUE 30. Colonies are streaked on an agar plate in different quadrants in order to dilute
the inoculum allowing for well-separated colonies to grow within the agar.

III. HEADINGS

A. Given the following images, determine the growth characteristics of the organism
in Thioglycollate broth.

A. Facultative anaerobic C. Microaerophilic

B. Strictly aerobic D. Strictly anaerobic

31. A
32. C
33. B
34. D

B. Determine the correct incubation technique that should be employed in

cultivating the following organism.

A. B. C.

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 A 35. Mycobacterium tuberculosis
B 36. Haemophilus influenzae
A 37. Escherichia coli
C 38. Actinomyces israelli
C 39. Clostridium tetani
B 40. Cardiobacterium hominis

IV. MATCHING TYPE:

A. Match the component from Column A to the correct culture media in Column B.
Then match the culture media to the organism from Column C.

Column A Column B Column C

(Component) (Culture Media) (Organism)
C 41. Trimethoprim A. Chocolate Agar 51. d a. Bordetella pertussis
Lactate
I 42. Malachite Green B. Mannitol Salt Agar 52. e b. Corynebacterium
diphtheriae
H 43. Phenol Red C. Modified Thayer Martin 53. i c. Vibrio parahaemolyticus
Agar
G 44. Bromthymol Blue D. Cefsulodin-Irgasan- 54. c d. Neisseria gonorrhoea
Novobiocin Agar
J 45. Sodium Tellurite E. Bordet-Gengou 55. b e. Mycobacterium
tuberculosis
E 46. Potato Glycerol F. Bile esculin agar 56. a f. Aeromonas hydrophilia
B 47. 7.5 % Sodium G. Thiosulfate Citrate Bile 57. g g. Staphylococcus aureus
Chloride Salt Sucrose
D 48. Novobiocin H. Xylose Lysine 58. f h. Haemophilus influenza
Desoxycholate Agar
A 49. 5% horse blood I. Middlebrook 7H10 Agar 59. h i. Salmonella arizonae
F 50. Ferric citrate J. Cystine Tellurite Agar 60. j j. Enterococcus faecalis

B. Match the following notable characteristic to the right organism.

A B
(Notable characteristic) (Organism)
E 61. Medusa head on BAP A. Actinomyces israelli
F 62. Satellitism B. Moraxella catarrhalis
A 63. Molar tooth C. Eikenella corrodens
H 64. Cauliflower-like colonies D. Staphylococcus aureus
K 65. Bull’s eye on CIN E. Bacillus anthracis
G 66. Brick red F. Haemophilus influenzae
D 67. Old sock odor G. Serratia marcescens
C 68. Bleach-like odor H. Mycobacterium tuberculosis
B 69. Wagon wheel colonies I. Pseudomonas aeruginosa
I 70. Green metallic sheen on BAP J. Yersinia enterocolitica
K. Escherichia coli

V. MODIFIED SEQUENCING:

For 71-80, select all the correct statements regarding the steps in the preparation of
Chocolate agar. Arrange the correct procedures in chronological order. Use
CAPITAL LETTERS only. NO spaces. (e.g. D,B,A,F,H)

**Manufacturer’s instructions: 40 grams for each 1000 mL water

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 A. Suspend 4 grams in 500 ml of distilled water in an Erlenmeyer flask.
B. Sterilize the media by autoclaving at 15 lbs pressure (121degC) for 15 minutes.
C. Store the plated media at 37degC when not in use,
D. Suspend 40 grams in 1000 ml of distilled water in an Erlenmeyer flask
E. Wait for the culture media to cool down until the heat is gone.
F. Weigh 40 grams of the dehydrated culture medium.
G. Allow solidifying at room temperature.
H. Sterilize the media by autoclaving at 112degC 15 lbs pressure for 51 minutes.
I. Sterilize all the glassware needed for the preparation of culture media.
J. Add defibrinated blood in proportion of about 5% and mix.
K. Dispense the culture media without enrichment in the petri dishes.
L. Store the plated media at 2-5degC when not in use.
M. Boil to dissolve the medium completely in a boiling waterbath.
N. Dispense the culture media aseptically in the Petri dishes.

ANSWER: I,F,D,M,B,J,N,G,L

VI. ANALYSIS:

81. For numbers 81-85, analyse the given 82.

images and spot if an error was
committed. Identify the error, otherwise
write NONE.

The label is on the wrong side of the

petri dish

NONE
83. 84.

The lid or cover is not on the petri dish

The tube cover is on the bench top or

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 working station.

85. 86. What special structure is shown in the

picture?

Metachromatic granules

Tap water was used instead of

distilled water. 87. What is the special stain used?
Toluidine blue

88. What organism have this special

structure?
Corynebacterium diphtheriae

89. Identify the colorless structure. 91. Identify the pointed structure.

Endospores

90. Give one genus of organism that have Capsule

this particular structure?
92. What stain is used?
Clostridium, Bacillus
India ink/Nigrosin dye

93. Give one organism that have this

particular structure.

Streptococcus pneumoniae

94-95. What is the most probable reason behind this gram reaction of a colony
incubated after 16 hours?

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The presence of two different organism indicates the possibility of contamination from
the sample collected, the prepared media or through the inoculation process.

EVALUATION: Evaluate the given colonies according to the different colony

characteristic.

96-100. A.
FORM: circular
SURFACE TEXTURE: smooth
COLOR: white
ELEVATION: concave
DENSITY: opaque

B.

101-105.
FORM: circular
SURFACE TEXTURE: smooth
COLOR: white
ELEVATION: umbonate
DENSITY: translucent

106-110. C.
FORM: filamentous
SURFACE TEXTURE: rough
COLOR: white
ELEVATION: flat
DENSITY: opaque