LC-4000 Series

Integrated HPLC and UHPLC systems

 A quick brief - LC-4000 Series
The LC-4000 Series is the latest in a long history of innovative HPLC
systems developed by JASCO reaching all the way back to the start of
commercial HPLC in the early 1970s.

The concept of the integrated LC-4000 series HPLC provides key

separation platforms at 50MPa, 70MPa and 130MPa which correspond
to conventional HPLC, the increasingly popular Rapid Analysis
(RHPLC) and sub 2um UHPLC, respectively.

Each platform is supplied with a dedicated pump and autosampler

matched to the operating pressure and all three platforms share
common detectors optimized for high-speed 100Hz acquisition and the
narrow peak shapes common to both RHPLC and UHPLC.

Advances in Solvent Delivery

For over two decades JASCO analytical HPLC pumps stability in solvent delivery across the entire analytical flow
have employed an asymmetric twin-piston solvent delivery rate range used in the PU-4180/85 RHPLC and PU-4280/85
system SSQD (Slow Suction, Quick Delivery) which provides UHPLC pump models.
significantly better flow and pressure profiles than the
commonly used conventional twin-piston reciprocating
design. In the LC-4000 series, SSQD has been re-developed,
with a completely new solvent delivery mechanism ExReFT
(Extremely Reliable Flow Technology) offering the highest

2
Pioneering Optical Design New Technologies for Method Development
As a pioneer in optical spectroscopy, JASCO has access With the range of column technologies becoming ever more
to optical designs that few can match. Adopting designs diverse - the traditional 5um fully porous particle is finally
from some of our more powerful spectrometers we have losing ground to some of the newer technologies such as the
developed a range of HPLC optical detectors with unrivalled new superficially porous solid core (Coreshell) and the wide
performance, like the new class leading FP-4020 fluorescence variety of sub-2um particles, the requirements for method
detector with S/N of over 2300:1, the latest high-speed development are becoming more demanding. The LC-4000
refractive index detector with micro cell optimized for RHPLC series has a number of newly developed accessories to help
and UHPLC and a completely revised circular dichroism you make better separations faster, with accessories such
detector for chiral chromatography. as, flexible and stackable column ovens in a range of sizes to
accommodate analytical to preparative columns and multiple
columns at every scale and with options for integrated 6 or 10
solvent and column selection valves.

ChromNAV, the cross-platform chromatography

data system, is evolving too. Version 2.0 now offers
many new features and a great new look to make
working with the LC-4000 HPLC system an even
better user experience.

Compact and Easy to Use

Despite the extra power delivered by the LC-4000 series
HPLC, the standard footprint is only 300 mm wide, a standard
system requires only a very small amount of bench space.

For easy user-maintenance, all LC - 40 0 0 instruments

feature front-access for replacing consumables such as
pump components and light sources, even the autosampler
consumables such as the syringe parts are easy to access.

For those users who require front-panel control, the LC-

4000 series retains the popular keypad and display (with a
backlit LED display as standard). Keypad control can still be
configured for convenient override when under PC control.

Each LC-4000 module

is designed for easy
maintenance.
Front-panel access to light
Front panel control is included as standard
source and consumables

The LC-4000 Series pump modules requires smaller bench

space than ordinary HPLC pumps. An integrated solvent
delivery system can be incorporated into a single pump
module.

3
 System Configurations
The LC-4000 series HPLC analytical and preparative HPLC systems have a number of
common features

300mm wide footprint

Each system component is completely stackable into a modest height
The system is fully integrated with complete system monitoring
All user replaceable components are accessible through the front panel
Vertical leak/drain system to channel away solvent
Integrated earthquake securing option
All detectors include 100Hz acquistion with interchangeable flowcells

Conventional HPLC and RHPLC Analytical Systems up to 70MPa

The LC-4000 Series 70MPa system has been designed to future-proof your HPLC
requirements. The LC-4000 70MPa system can be used with conventional 3 and 5µm
particle size analytical columns at typical lower pressures around 10 to 20MPa (1500
to 3000psi) and can also be used with smaller particles such as shorter length UHPLC
columns and superficially porous (SPP) or Coreshell that require slightly higher
pressure solvent delivery to provide optimum linear velocity through the column.

UHPLC Analytical Systems at 130MPa

Ten years after the new segment of the HPLC market, UHPLC has found a niche for
those that need to run a large number of samplers or get results quickly. Pioneering
column technology is pushing the particle size even smaller. The near 20,000 psi
operation of the LC-4000 130MPa is designed to take advantage of the separation
efficiency of the very small particles couple with longer and narrower columns. The
LC-4000 130MPa system incorporates many of the same features as the 70MPa
system, but with materials designed to withstand higher pressures.

Preparative HPLC Systems

The LC-4000 preparative HPLC systems includes three platform options

Up to 20mL/min for columns up to 21.2mm ID

Up to 50mL/min for columns up to 3cm ID
Up to 120mL/min for columns up to 5cm ID

Large column oven for multiple preparative columns and optional automatic column
switching
Preparative detector options including high flow short path length flow-cells
Open-bed fraction collector with sample trays for tubes, plates or large containers
ChromNAV-FC for fraction control using time, threshold or slope from up to 4 different
signals

Semi-prep HPLC LC-4000 system

4
LC-4000 Series Extensive Range of Modules
The LC-4000 Series, enables multiple system design based on the following modules. This concept can be used to create many
different system configurations to match your application.

The extensive range of detectors based on JASCO's spectroscopy technology is a key factor in providing a solution to virtually any
application.

PUMPS

HPLC, RHPLC UHPLC PREP

PU-4185/80 PU-4285 PU-4086/87
PU-4185B PU-4285B PU-4086B

DETECTORS

UV/Vis visible PDA Refractive

UV-4070/75 MD-4010/15/17 Index
RI-4030/35

Fluorescence Circular Dichroism Optical

FP-4020/25 CD-4095 rotation
OR-4090

AUTOSAMPLERS

RHPLC
AS-4150
HPLC
AS-4050/50i UHPLC
AS-4250

Preparative
AS-4058

COLUMN OVENS

CO-4060 CO-4065 Prep

high capacity

CO-4061/62 RO-4068
compact Reaction oven

5
The LC-4000 Series next generation HPLC pumps
with flexible expandability
The LC-4000 series HPLC pumps include expansion bays that allow a simple pump module to be transformed into a powerful
solvent delivery system. Accessory modules can be installed in the LC-4000 series pump for use with virtually any solvent
delivery requirement.

Upgrade and Expansion

The unique expansion bays in the pump platform can be fitted with plug-in
modules to quickly and easily transform a simple isocratic pumping system
into a sophisticated multi-solvent delivery system by installing a second pump,
degasser, quaternary gradient unit, solvent selection valve or dynamic mixer
without increasing the footprint.

Adding a second pump module enables an isocratic pump to be upgraded to a

dual pump system for either binary gradient or two isocratic flows.

A powerful configuration of the single-unit design is the quaternary gradient

PU-4180 solvent delivery system including degasser, with a post-column reagent pump
Analytical pump platform configured in a compact chassis only 300mm wide.

PU-4180 Flow rate accuracy

(1.0mL/min, n=10)

Mobile phase: Acetonitrile/Water (80/20)

Flow Rate: 1.0mL/min
Column: CrestPak C18S (4.6mmI.D. x 150mm, 5µm)
Column temerature: 40 deg C
Detection wave length: 260nm, Response; 1sec
Sample: Polycyclic aromatic hydrocarbon (10µg/mL each)
(Naphthalene, Fluorene, Anthracene, Pyrene, Chrysene, Benzo[a]pyrene)
Injection: 10µL

Retention time reproducibility (n=10)

# Naphthalene Fluorene Anthracene Pyrene Chrysene Benzo[a]pyrene
1 3.1400 3.9367 4.5000 5.6283 6.4934 9.4267
2 3.1417 3.9683 4.5017 5.6317 6.4950 9.4300
3 3.1417 3.9367 4.5000 5.6300 6.4933 9.4285
4 3.1400 3.9367 4.5002 5.6283 6.4950 9.4283
5 3.1400 3.9367 4.5000 5.6283 6.4950 9.4283
6 3.1400 3.9367 4.5000 5.6300 6.4933 9.4283
7 3.1416 3.9383 4.5016 5.6317 6.4967 9.4317
8 3.1400 3.9367 4.5000 5.6300 6.4950 9.4317
9 3.1400 3.9367 4.5000 5.6300 6.4949 9.4317
10 3.1400 3.9367 4.5017 5.6301 6.4950 9.4317
Average 3.1405 3.9370 4.5005 5.6299 6.4947 9.4297

PU-4185 Standard diviation (n-1)

CV% (n-1)
0.001
0.025
0.001
0.018
0.001
0.017
0.001
0.022
0.001
0.016
0.002
0.020

Semi micro pump platform

Eliminating pulsation
The problems caused by pressure achieve with commercially available The PU-4280 and PU-4285 UHPLC
variations in solvent delivery when pumps). The PU-4180 and 4185 pumps pumps provide precise flow at pressures
switching between the two pump heads operate at pressures up to 70 MPa, up to 130 MPa; for columns with particle
has been improved with a new design designed mainly for use with RHPLC sizes of less than 2 µm. The LC-4000
that precisely controls the plunger for core-shell columns or similar micro series UHPLC system can also be used
drive. The LC-4000 series analytical packing materials as well as working for rapid analysis HPLC (RHPLC) and
pumps offer flow rate precision with at pre s sure s c o mm o nly use d fo r conventional HPLC.
RSDs of better than 0.05% (difficult to conventional HPLC.
6
Pump Configurations
The PU-4180/85 and PU-4280/85 analytical pumps can
be configured for both single pump gradient and multiple
pump gradient operation. The single pump quaternary
gradient module offers excellent gradient formation for up to
four solvents. The newly designed 4-way solvent valve for
switching the four solvents is synchronized to the operation
of the pump motor control - variable cycle adaption
optimizes delivery according to gradient resolution and flow PU-4185-Binary pump
rate. This highly efficient quaternary gradient mode can
be applied to a wide flow-rate range from low to high flow Two pump binary gradient
rates.
Two and three pump binary or ternary gradient modules
with optional built-in degasser are available for fast cycle
high-resolution gradient formation; the response time is
superior to the single pump quaternary gradient system.

Solvent Mixing
Multi-pump gradient mixing is done using a variable speed
dynamic mixer with a range of a small volume mixing
chambers incorporating a micro-stirrer to provide the
smallest possible dead volume, offering higher mixing
efficiencies for all HPLC solvents.

The optional in-line TERA Mixer is a turbulent-flow solvent

mixer uniquely designed to offer high efficiency mixing with
minimal dead volume. PU-4180 Quaternary gradient mixing performance
(1.0mL/min, n=10)
Solvent Degassing
Mobile phase:A;
Water, B; Acetonitrile
Gradient: (A/B) (65/35) → (5/95) Linear Gradient
The in-line vacuum degasser module uses a new Teflon Flow rate: 1.0mL/min
AF membrane with a hold-up volume of only 400µL (per Column: CrestPak C18S (4.6mmI.D. x 150mm, 5µm)
Column temerature:
40 deg C
solvent line); this offers very quick solvent replacement with Detection wave length:
254nm, Response; 1sec
minimal waste. Despite its small size, the new degasser Sample: Alkylphenon 10µg/mL

offers more effective and stable degassing than larger (Acetanilide, Acetophenone, Propiophenone,
Butyrophenone, Valerophenone, Hexanophenone,
previous generation models. A degasser for preparative- Heptanophenone,Octanophenone, Benzophenone (5µg/mL))
HPLC is also available. Injection: 10µL

Retention time reproducibility (n=10)

# Acetanilide Acetophenone Propiophenone Butyrophenone Benzophenone Valerophenone Hexanophemone Heptanophenone Octanophenone
1 3.117 5.617 7.590 9.303 9.950 10.980 12.583 14.067 15.430
2 3.117 5.617 7.590 9.307 9.950 10.983 12.587 14.067 15.433
3 3.113 5.617 7.590 9.307 9.950 10.983 12.587 14.067 15.433
4 3.117 5.617 7.590 9.307 9.953 10.983 12.587 14.067 15.433
5 3.117 5.617 7.590 9.307 9.953 10.983 12.587 14.067 15.433
6 3.117 5.620 7.593 9.310 9.953 10.987 12.587 14.067 15.433
7 3.117 5.620 7.590 9.307 9.953 10.983 12.587 14.067 15.433
8 3.117 5.617 7.590 9.310 9.953 10.987 12.590 14.067 15.433
9 3.117 5.617 7.593 9.310 9.957 10.987 12.593 14.073 15.437
10 3.117 5.620 7.593 9.313 9.957 10.990 12.593 14.073 15.437
Average 3.116 5.618 7.591 9.308 9.953 10.985 12.588 14.070 15.434
Standard diviation (n-1) 0.001 0.002 0.002 0.003 0.002 0.003 0.003 0.002 0.002
CV% (n-1) 0.034 0.029 0.021 0.030 0.025 0.026 0.026 0.016 0.012

PU-4180
(compact quaternary gradient pump
with micro-volume degasser ) *Option

Preparative and Semi-preparative Pumps

The LC-4000 Preparative-HPLC so that the maximum pressure
System includes pumps at three can be used at higher flow-rates
scales. The PU-4086 pump offers for all pump models. Solvents can
flow rates up to 20 mL/min, the be delivered at higher flow rates
PU-4087 pump up to 50 mL/min and higher pressures for use with
and the PU-4088 up to 120mL/ longer preparative HPLC columns
min. Both single and multi-pump that generate more back pressure
gradient modes can be used with and for faster and more productive
the PU-4086 pump platform whilst preparative separations.
the PU-4087 and PU-4088 are Dynamic mixers, solvent selection PU-4086 semi prep pump
used in multi-pump mode only. valves and the prep degasser can Recycling inlet switching unit, Recycling valve
The new MK2 technology used in be installed in the expansion bays unit (Option)
the PU-4086 and PU-4087 motor in both the PU-4086 and PU-4087
driver control has been improved modules.

7
 A comprehensive range
of high performance optical detectors
With JASCO’s long history of spectrometer development, you will find that each detector is well designed; these
next generation detectors employ the latest optical and electronic technologies for high sensitivity, excellent
stability and easy maintenance.

MD-4010, MD-4015 900nm with a resolution of 1nm and

and MD-4017 fast spectrum acquisition rate of up
to 100 spectra/sec. When used with
PDA Detectors the new temperature controlled SP
type semi-micro flow cell the MD-
The LC-4000 Series includes three 4010 is well suited to the narrower
PDA detec tor s. The M D - 4017 is peaks typically found with UHPLC or
a useful replacement for a single RHPLC separations. The MD-4010 is
wavelength detector, the MD- 4015 fitted with an internal mercury lamp for
i s t h e wo r k h o r s e f o r U V- v i s i b l e automatic wavelength calibration.
detection in the range 200 to 600nm
and the MD-4010 is designed for high The MD- 4015 PDA detector is the
sensitivity and high spectral and time routine detector for conventional HPLC,
resolution. RHPLC and UHPLC. The sensitivity is
almost equivalent to that of the MD-
The MD-4010 PDA detector offers the 4010 but only covers the wavelength
MD-4010 PDA highest detection sensitivity, equivalent range from 200nm to 600nm using a
to a single wavelength detector. Making 512 element PDA for 1nm resolution
use of a 1024 element photometric and with high speed 100Hz spectrum
d i o d e a r r a y, t h e m e a s u r e m e n t acquisition.
wavelength range is from 190nm to

The MD-4017 detector has a price-point

comparable to a typical single wavelength detector
and has been designed for the user only interested
in the UV region from 200-400nm, the resolution
is excellent at 1nm and the maximum spectrum
acquisition is 20 spectra per second (20Hz), useful
for peak widths as small as 1 to 2 seconds.

When used with ChromNAV 2.0 CDS the PDA

detector offers multi-functional analysis with a
ChromNAV PDA data processing Front panel access for easy variety of functions such as spectral analysis,
included as standard lamp replacement library search for peak identification, peak purity
and multi-wavelength quantitation.

UV-4070/4075 simultaneous dual-wavelength data

UV-Visible Detectors acquisition and spectral scanning.
Us e r mainte nanc e c an b e d o ne
Using the advanced Czerny- without removing the module from
Turner monochromator design with the system. Front-panel access is
optimization of the optical system, used for light source replacement and
baseline change due to refractive index automatic wavelength calibration can
is minimized. Baseline fluctuation from be carried out using a mercury lamp.
changes in environmental temperature Both detectors include high speed data
i s r e d u c e d w i t h a te m p e r at u r e - acquisition at up to 100Hz and can
controlled cell, baseline drift caused be used for conventional HPLC with
by temperature fluctuation in the the standard flow cell and for UHPLC/
UV-4070 UV Visible
light source is also reduced with a RHPLC when used with the SP type
temperature controlled light source. semi-micro cell.
UV spectra auto-scan
The result is excellent stability with
Automatic spectral scannning can be the noise level +/- 0.2x10 -5 AU and
controlled using ChromNAV, when a drift 1x10 -4 AU/h. Both detectors offer
peak exceeds a set threshold a scan is
executed automatically.
220000
0.24
200000
0.2
Intensity[µV]

Abs
0.1
100000

Threshold 50000 UV detector Auto scan parameters

0
-0.01
200 400 600 800 900
Threshold 10000
Wavelength[nm]
8 0

0.0 2.0 4.0 6.0 8.0 10.0 12.0 14.0

Spectral measurement view
Retention Time[min]
 FP-4020/4025 Fluorescence detector
The FP-4020 fluorescence detector has been designed for the pursuit of extreme
sensitivity. Re-designing the optics and electronics from the ground up, as well as
removing the effects of ambient temperature with a new temperature-regulated
cell body has led to the highest sensitivity with stable detection. The new axially
irradiated cell design enables the very highest sensitivity detection. These
changes have resulted in a giant leap in sensitivity for the FP-4020 to achieve
a signal noise ratio of 2300:1 or higher (based on the water Raman peak). The
FP-4025 is an excellent routine fluorescence detector that has a slightly lower
specification with a water Raman S/N of 1400:1 .
Both detectors can be used for simultaneous detection at two wavelength pairs;
excitation and emission wavelengths can be changed during the acquisition using
FP-4020 Fluorescence a time program and automatic spectra scanning can be triggered manually, by
time or automatically by threshold. A built-in mercury lamp enables automatic
wavelength correction and the front-access design allows easy replacement of the
flow cell assembly and light source.

26000

20000 Anthracene 1.4 fg (S/N=3)

(Anthracene 100 fg Injection)
Intensity[µV]
Intensity [µV]

10000

0
0.0 1.0 2.0 3.0 4.0 5.0
0.0 1.0 2.0 3.0 4.0 5.0
Retention Time[min]
Retension time [min]

High sensitivity measurement of Anthracene Dual wavelength simultaneous measurement

Refractive Index detector RI-4030/4035

The Refractive Index detectors include the optical system mounted in a precisely
temperature-regulated housing to eliminate the influence of changes in ambient
temperature. The RI-4030 and 4035 detectors are fully stable just one hour
after powering on. The RI-4030 can be used for both analytical and preparative
scales. In preparative mode the maximum flow rate is 120 ml/min. The RI-4035
is a dedicated semi-micro RI detector with a micro-volume flow cell designed for
use with semi-micro GPC analysis using GPC columns with a fine pore size. The
100Hz data acquisition rate makes the RI-4035 suitable for RHPLC and UHPLC
separations. The LED light source in both detectors has a long life time (over
RI-4030 Refractive Index 10,000 hours) for low maintenance operation. Both the RI-4030 and RI-4035
detectors can be fitted with an optional pressure release safety-valve to prevent
damage to the flow cell in the event blockage.

Circular Dichroism detector CD-4095

The CD-4095 is a unique HPLC detector for measuring circular dichroism
developed from the market leading J-Series circular dichroism spectrometer.
Optically active compounds with a chromophore close to a chiral center may
absorb circularly polarized light, which can be detected with excellent sensitivity
and selectivity. When chiral compounds are measured using a UV-Visible
detector, the d- and l- enantiomers cannot be distinguished even when separated
by a chiral column. The CD-4095 circular dichroism detector makes it possible
to positively distinguish between the d- and l- enantiomers by measuring the
positive and negative peaks. It is also possible to quantify the d- and l-forms even
when they are not separated, a calibration curve based on the g-factor can be
CD-4095 Circular Dichroism used to determine the enantiomeric ratio. The ChromNAV CDS can be used to
simultaneously measure the CD, g-factor data and UV-visible signal. The CD
spectra of eluted compounds can be measured by triggering scanning manually,
by timed program or automatically by threshold.

Chiral detector OR-4090

The OR-4090 Chiral detector measures the angle of rotation of plane polarized
light caused by optical active isomers and is useful for chiral compounds with no
absorption. The OR-4090 uses a high intensity Hg-Xe arc lamp (150W) providing
a strong output in the UV/Visible region where the largest optical rotations offer
the highest sensitivity. The wide wavelength range from 350-900nm also allows
measurement of a wide variety of samples. One of the key benefits of optical
rotation detection is that it is unaffected by changes in solvent and can be used
with gradient elution, unlike an RI detector.
OR-4090 Optical Rotation 9
 Autosamplers
The LC-4000 Series autosamplers provide a range of options to suit any sample
injection requirements, from the AS-4050 entry-level model, to the AS-4250 UHPLC
model or the AS-4058 preparative scale autosampler.

The AS-4050 is a 60 sample autosampler for use with conventional HPLC with options
for analytical and semi-preparative injection volumes. The AS-4150 and AS-4250 are
high-capacity, high performance autosamplers designed for RHPLC (up to 70MPa)and
UHPLC (up to 130MPa), respectively, with a minimum injection cycle-time of only 30
seconds. Peltier cooling is available for all autosamplers and light protection for photo- AS-4050
labile samples is available on the higher level models. 60 sample HPLC autosampler
Pre-injection sample preparation is available on the high performance autosamplers
with programmable options for sample dilution and pre-column derivitization.

The injection mode can be switched between full loop injection and partial loop
injections.

Up to 5 different washing solvents can be used to achieve excellent carry-over at less

than 0.005%.
An optional degasser can be installed for degassing up to 5 flushing solvents.

The AS-4058 is the LC-4000 Series largest volume autosampler with injection volumes
up to 5mL as standard (up to 10mL as an option). The AS-4058 is typically used in
combination with either a PU-4087 or PU-4088 pump for preparative separations.

All LC-4000 series autosamplers share the same simple maintenance features, with
consumable parts such as injection ports, rotor seals, and loops accessible from the AS- 4250
front panel for easy replacement. High capacity UHPLC
autosampler

Simple replacement of
syringe in AS-4050

Designed for easy maintenance

Metering Tube

Air-drain
Valve Waste
Bottle Needle

Solvent-stop Flushing
Port Sample
Valve
Vial
NC Injection
4 Port
Air Pump 5
IN
Sample
NO Column
loop
To Detector
6 3
3way
Valve
1
2

Injection
Valve HPLC Pump

Flushing Metering
Solvent Pump

AS-4050/4150/4250/4058 flow diagram (loop injection)

10
 Column Ovens
The LC - 40 0 0 Ser ies c olumn ovens lengths up to 300mm and a pair of column
have been designed to provide excellent selection valves can be equipped (option).
utility for a wide range of different user
requirements. For simple requirements All column ovens have built-in electronic
the stackable CO-4061 can be used with cooling for controlling column temperature
column lengths up to 200mm (or up to at sub-ambient temperatures.
CO-4061 300mm with an optional extension).
The CO-4062 and CO-4061 block-heater
For RHPLC and UHPLC the CO-4062 type ovens can be fitted with an optional
has c apac it y for up to six 15 0 mm micro-volume pre-heating unit for pre-
length columns, with optional one or two heating the mobile phase before the
internally mounted automatic switching column to improve resolution and peak
valves for 2 or 6 column selection. shape.

For GPC and larger preparative columns, The RO-4068 reaction oven is used for
select either of the two high-capacity high temperature post-column sample
C O - 4 0 6 0 o r C O - 4 0 6 5 ove n s . T h e processing with capacity for columns and
CO- 4060 can accommodate columns reaction coils.
with lengths up to 300mm or larger ID
preparative columns. The CO-4065 can
accommodate up to 10 columns with

CO-4060
600000

Toluene With Pre coulmn stabilizer —

Temp : 25 degree C Without Pre coulmn stabilizer —
400000
Intensity

Column X-Presspak VC18-W

(3 mmI.D. x 50 mmL, 2 µm)
200000
Eluent Water / Acetonitrile (Gradient)
Flow Rate 1.0 mL/min
0
Sample Gasoline Oxidization Products
0.0 0.4 0.8 1.2 1.6 2.0 2.4 2.8 Analysis conditions
600000

Toluene With Pre coulmn stabilizer —

Temp : 40 degree C Without Pre coulmn stabilizer — Room Oven Pre-Column Theoretical Peak Width tR(min)
400000 Temp. Temp. Stabilizer
With 56095 0.019 1.893
Intensity

25˚C
Without 54747 0.019 1.887
21˚C
200000 With 46021 0.020 1.799
40˚C
Without 36402 0.023 1.851

0
Comparison of Toluene peaks
0.0 0.4 0.8 1.2 1.6 2.0 2.4 2.8

Efficiency comparison of stabilizer

UniFinePak HPLC Columns

UniFinePak is a new type of column that uses a hybrid packing material. Compared
with traditional packing materials that use silica as the base material, UniFinePak
columns can be used with a wider pH range from alkali to acid. UniFinePak columns
offer an extended analysis range due to its chemical strength and resilience to high
pressure operation.
The entire range of UniFinePak columns uses a common packing material in a range of
particle sizes from semi-micro to analytical to semi preparative scales, which simplifies
method development when scaling-up separations from analytical to semi-preparative .

Optional Preparative HPLC fraction collection kit Method Conversion Program

The LC-4000 series preparative system fraction collection kit includes
Fraction collector
Flow divertor valve
ChromNAV FC fraction control program for ChromNAV

11
 ChromNAV 2.0 The 'PowerTool' for HPLC Analysis
ChromNAV 2.0 (and ChromNAV-CFR 2.0) are JASCO’s next generation CDS developed from the powerful and easy-to-use
ChromNAV 1.0 with a host of exciting new features.

With a customizable graphical-user-interface (GUI), the user can set-up the system to display only the functions necessary for
their application. This latest intuitive GUI allows the user to quickly learn the operation and explore the extensive functionality
for data processing.

ChromNAV 2.0 is a universal CDS which can be used with any type of separation - HPLC, UHPLC, RHPLC, prep-HPLC,
analytical SFC and prep-SFC. ChromNAV can also satisfiy the demands of dedicated analysis and multi-purpose systems.

Control method

The pump flow rate and gradient profile display is

flexible and can be overlaid with a chromatogram
for adjusting gradient conditions.

PDA analysis

PDA data processing is included as standard. Data is

displayed in 2D contour plot and 3D with simultaneous overlay
of spectra and chromatograms.Extract chromatograms at
single or multiple wavelengths for quantitation

ChromNAV 2.0 offers powerful system Previously acquired chromatograms A newly added feature of ChromNAV
control and data acquisition with can be overlaid for visual comparison 2.0 is the automatic message that can
options for 2D (or up to 4D) gradient with the data currently being acquired, be emailed to your PC or smartphone
control and multi-zone oven control as a quick indicator to the user of to give an update on the progress of
with control of up to 3 column ovens current separation performance. the sequence.
for method development. Solvent and
column selection control is increased Data acquisition and analysis for With the increasing requirements for
to include up to 10 positions for both. a PDA detector are included as Green Energy, the LC-4000 series
standard in ChromNAV. has the facility not only to power off
During acquisition, the run-time for a and save lamp life at the end of the
chromatogram can be increased to The fluorescence and UV-visible run, but also a ‘soft-off’ function to
capture later eluting peaks which may detectors include spectral scanning automatically power off a module at
exceed the set run-time. as standard, which can be triggered the end of the sequence.
manually, by time or by threshold.

12
ChromNav 2.0 standard features
• Peak integration and peak identification
• Peak grouping
• Linear and non-linear quantitation
• 3D chromatogram analysis,
• Spectral analysis for UV-visible, Fluorescence and PDA detectors
• Style report generator
• User calculation

All raw data is protected and saved; which can then be analyzed and re-analyzed, reported and saved with both the raw
data and with any data processing from the user’s analysis.

A comprehensive audit trail records the acquisition method along with a history of instrument performance indicators in each
sample data file, which provides the user a snap-shot of the condition of the system during the run and can warn about
requirements for impending maintenance.
ChromNAV 2.0 Optional Applications
• ChromNAV CFR for 21 CFR Part 11 compliance and electronic registration of data
• GPC molecular weight dispersion
• Molecular weight dispersion of low molecular weight heparin
• FUMI theory (Function of Mutual Information) for theoretical precision analysis

ChromNAV-GPC

Molecular weight distribution program ( Option )

ChromNAV GPC/SEC calculation program for GPC analysis
ChromNAV- FC Collection view window (Option)

ChromNAV-FC fraction collector control for the CHF-122SC

fraction collector

ChromNAV-FC for preparative HPLC

The optional ChromNAV-FC fraction or circular dichroism. Fraction
collector control is available as simulation is applied to an acquired
part of the Prep-HPLC kit and chromatogram to optimize the
provides ChromNAV with the fraction collection parameters and
capability to control the LC-4000 the graphical display can be used
Series preparative HPLC system for to locate peaks of interest in the
fraction collection. fraction collector tray.

ChromNAV-FC offers control for ChromNAV-FC also supports

two different capacity fraction additional functions for prep-HPLC
collectors, with sophisticated applications, including automatic
fractionation control with several multi-channel fractions, fraction
methods for peak collection, such repeat and fraction re-cycle.
as time, threshold and slope, and
can even be programmed to use *1: ChromNAV CFR is an optional
data collected simultaneously software package for ChromNAV.
from multiple detectors such
as UV-visible, refractive index

13
 Specifications
Pumps
Model PU-4185 PU-4180 PU-4285
Flow rate setting range 0.001 ~ 4.0 mL/min 0.001 ~ 10.0 mL/min 0.001 ~ 2.0 mL/min
Applicable flow rate 0.05 ~ 3.0 mL/min (~ 70 MPa) 0.5 ~ 6.0 mL/min (~ 70 MPa) 0.050 ~ 1.5 mL/min (~ 130 MPa)
range ~ 4.0 mL/min (~ 40 MPa) ~ 10.0 mL/min (~ 35 MPa) 1.501 ~ 2.0mL/min (~ 100 MPa)
70 MPa (~ 3.0 mL/min) 70 MPa ( ~ 6.0 mL/min) 130.0 MPa (~ 1.5 mL/min)
Maximum pressure
40 MPa (~ 4.0 mL/min) 35 MPa (~ 10.0 mL/min) 100.0 MPa (~ 2.0 mL/min)
+/- 1% or +/- 2 µL/min, whichever is larger. +/- 1% or +/- 2 µL/min, whichever is larger. +/- 1% or +/- 2 µL/min, whichever is larger.
Flow rate accuracy
(0.05 ~ 4.0 mL/min) (0.5 ~ 10.0 mL/min) (0.05 ~ 2.0 mL/min)
0.05% RSD or +/- 0.04min SD, whichever is larger. 0.05% RSD or +/- 0.04min SD, whichever is larger. 0.05 % RSD or +/- 0.04 min SD, whichever is
Flow rate precision (0.05 ~ 4.0 mL/min) (0.5 ~ 5.0 mL/min) larger. (0.1 ~ 2.0 mL/min)
(measurement by chromatogram) (measurement by chromatogram) (measurement by chromatogram)
Dimensions, weight 300(W) x 470(D) x 150(H) mm, 13 kg 300(W) x 470(D) x 150(H) mm, 13 kg 300(W) x 470(D) x 150(H) mm, 14.5 kg
Power requirement AC 100 ~ 240 V, 50/60 Hz, 80 VA AC 100 ~ 240V, 50/60 Hz, 80 VA AC 100 ~ 240V, 50/60 Hz, 60 VA

Gradient mode with low-pressure gradient unit

+/-0.6% (5 ~ 95%, 0.2 ~ 1.0 mL/min) +/-0.6% (5 ~ 95%, 0.2 ~ 1.0 mL/min)
Mixing accuracy +/-0.8% (5 ~ 95%, 0.5 ~ 5.0 mL/min)
+/-1.2% (5 ~ 95%, ~ 4.0 mL/min) +/-1.2% (5 ~ 95%, ~ 2.0 mL/min)
0.15% RSD or +/- 0.01min, whichever is larger. 0.25% RSD or +/-0.02min, whichever is larger. 0.15% RSD or +/-0.01min, whichever is larger.
Mixing precision (0.2 ~ 4.0 mL/min) (0.5 ~ 5.0 mL/min) (0.2 ~ 2.0 mL/min)
(measurement by chromatogram) (measurement by chromatogram) (measurement by chromatogram)

Binary pump PU-4185-Binary PU-4285-Binary

Gradient mode
+/-0.4% +/-0.4%
Mixing accuracy -
(5 ~ 95%, 0.2 ~ 4.0 mL/min) (5 ~ 95%, 0.2 ~ 2.0 mL/min)
0.15% RSD or +/-0.01 min, whichever is larger. (0.2 0.15% RSD or +/-0.01 min, whichever is larger.
Mixing precision ~ 4.0 mL/min) - (0.2 ~ 2.0 mL/min)
(measurement by chromatogram) (measurement by chromatogram)
Dimensions, weight 300(W) x 470(D) x 150(H) mm, 19.5 kg - 300(W) x 470(D) x 150(H) mm, 22.5 kg
Power requirement AC 100 ~ 240 V, 50/60 Hz, 130 VA - AC 100 ~ 240 V, 50/60 Hz, 110 VA

Model PU-4086 PU-4087

Flow rate setting range 0.001 ~ 20.0 mL/min 0.01 ~ 50.0 mL/min
5.0 ~ 30.0 mL/min (~ 50 MPa)
Applicable flow rate 0.5 ~ 20.0 mL/min
30.01 ~ 40.0 mL/min (~ 40 MPa)
range (0 ~ 50 MPa)
40.01 ~ 50.0 mL/min (~ 30 MPa)
50 MPa (~ 30.0 mL/min)
Maximum pressure 50 MPa 40 MPa (~ 40.0 mL/min)
30 MPa (~ 50.0 mL/min)
+/- 1% or +/- 10 µL/min, whichever is larger. +/- 1% or +/- 50 µL/min, whichever is larger.
Flow rate accuracy
(0.5 ~ 20.0 mL/min) (5.0 ~ 50.0 mL/min)
0.05% RSD or +/- 0.04min SD, whichever is larger. 0.1% RSD or +/- 0.04min SD, whichever is larger.
Flow rate precision (0.5 ~ 20.0 mL/min) (5 ~ 40 mL/min)
(measurement by chromatogram) (measurement by chromatogram)
Dimensions, weight 300(W) x 470(D) x 150(H) mm, 14.5 kg 300(W) x 470(D) x 150(H) mm, 18 kg
Power requirement AC 100 ~ 240 V, 50/60 Hz, 90 VA AC 100 ~ 240 V, 50/60 Hz, 120 VA

Binary pump PU-4086-Binary

Gradient
Mixing accuracy +/-0.75% (5 ~ 95%, 0.5 ~ 20.0 mL/min) -
0.2% RSD or +/-0.03min, whichever is larger.
Mixing precision -
(0.5 ~ 20.0mL/min)
Dimensions, weight 300(W) x 470(D) x 150(H) mm, 21.5 kg -
Power requirement AC 100 ~ 240 V, 50/60 Hz, 100 VA -

14
Detectors
Model MD-4010 MD-4015 MD-4017
Light source D2 & WI lamp D2 lamp D2 lamp
Measurement range 190 ~ 900 nm 200 ~ 600 nm 200 ~ 400 nm
PDA element number 1024 ch 512 ch 512 ch
Slit width 1, 4, 8 nm 4 nm 4 nm
Data acquisition rate Maximum 100 spectra/sec (full range) Maximum 100 spectra/sec (full range) Maximum 20 spectra/sec (full range)
Noise level +/- 3.0 x 10 -6 AU (specified conditions) +/- 3.0 x 10-6 AU (specified conditions) +/- 7.0 x 10-6 AU (specified conditions)
Drift < 0.5 x 10-6 AU/h (specified conditions) < 0.5 x 10-6 AU/h　(specified conditions) < 1.0 x 10-6 AU/h (specified conditions)
Linearity 2.0 AU or more (specified conditions) 2.0 AU or more (specified conditions) 2.0 AU or more (specified conditions)
Flow cell Path length: 10 mm, front loading cassette type Path length: 10 mm, front loading cassette type Path length: 10 mm, front loading cassette type
PC communication USB2.0 USB2.0 USB2.0
Dimensions, weight 300(W) x 470(D) x 150(H) mm, 14.5 kg 300(W) x 470(D) x 150(H) mm, 13.5 kg 300(W) x 470(D) x 150(H) mm, 13.5 kg
Power requirement AC 100 ~ 240 V, 50/60 Hz, 180 VA AC 100 ~ 240 V, 50/60 Hz, 150 VA AC 100 ~ 240 V, 50/60 Hz, 120 VA

Model UV-4070 / UV-4075

Light source D2 lamp + WI lamp (UV-4070)
D2 lamp (UV-4075)
Wavelength range 190 ~ 900 nm (UV-4070)
190 ~ 600 nm (UV-4075)
Monochromator Czerny-T urner mount
Spectral bandwidth 8 nm
Noise level +/-0.2 x 10-5 AU (230 nm, 1.5 sec)
Drift 1 x 10-4 AU/h (250 nm, constant room temperature)
Data output Maximum 100 Hz
Front loading cassette cell (temperature controlled), tapered.
Flow cell
Path length: 10 mm
Spectrum measurement 200 ~ 900 nm (D2 & WI), 200 & 370 nm (D2), 371 ~ 900 nm (WI) (UV-4070)
(On-flow measurement) 200 ~ 600 nm (D2) (UV-4075), applicable measurement range; fixed
Any two wavelengths in 190 ~ 370 nm, 371 ~ 700 nm, 701 ~ 900 nm (UV-4070)
T wo-wavelengths monitoring
Any two wavelengths in 190 ~ 370 nm, 371 ~ 600 nm (UV-4075)
Dimensions, weight 300(W) x 470(D) x 150(H) mm, 10 kg
Power requirement AC100 ~ 240 V, 50/60 Hz, 175VA (UV-4070), 110VA (UV-4075)

Model FP-4020 (high performance model) / FP-4025 (standard model)

Light source Xenon short arc lamp
Wavelength setting range 200 ~ 900 nm (both EX and EM)
(measurement range) 220 ~ 700 nm (both EX and EM), 200 ~ 900 nm (both EX and EM, option)
Spectral bandwidth EX: 20 nm, EM: 20, 40 nm (switchable)
Raman peak of water, S/N > 2300 (FP-4020)、 S/N > 1400 (FP-4025)
Sensitivity
(EX wavelength; 350 nm)
Data output Maximum 100 Hz
Flow cell Front loading cassette cell
T emperature control Available setting: OFF, 4 ~ 40ºC, applicable range: [ambient -10ºC] ~ 40ºC (only for FP-4020 )
Spectrum measurement Ex and Em spectrum measurement, spectrum output, output of subtracted spectrum
T wo-wavelength monitoring T wo sets of Ex/Em wavelength setting, maximum wavelength difference; 200 nm or shorter.
Dimensions, weight 300(W) x 470(D) x 225(H) mm, 20 kg
Power requirement AC 100 ~ 240 V, 50/60 Hz, 270VA (FP-4020), 230VA (FP-4025)

Model RI-4030 RI-4035 Model CD-4095

Measurement system Deflection type Light source 150W Hg-Xe lamp
Refractive index range 1.00 ~ 1.75 Polarizer Glan-T aylor prism

5.0 x 10-5 RIU (HIGH) 5.0 x 10-5 RIU (HIGH) Phase modulation PEM (photo-elastic modulator)
Wavelength accuracy +/- 5 nm
Linearity 5.0 x 10-4 RIU (ST D) 5.0 x 10-4 RIU (ST D) (H2O)
Wavelength reproducibility +/- 0.5 nm
-3
5.0 x 10 RIU (LOW) (H2O)
Wavelength setting range 200 ~ 460 nm
Noise level 0.20 x 10-8 RIU or less (HIGH, ST D) 0.50 x 10-8 RIU or less (HIGH, ST D) Measurement range 220 ~ 460 nm
Cell capacity 10 µL 2.7 µL Noise level 0.04 mdeg (at specified conditions)
Maximum usable flow- 10 mL/min (Low Flow Rate tubing) 0.1 mdeg/h (room temperature variation is
1.2 mL/min (H2 O) Drift
rate 120 mL/min (High Flow Rate within +/-1ºC)
0.1 MPa (Low Flow Rate tubing) Data output Maximum 100 Hz
Maximum pressure 0.1 MPa
0.3 MPa (High Flow Rate tubing) Front loading cassette cell, tapered, 44 µ
Flow cell
T emperature control Ambient +10ºC ~ ambient +25ºC (setting range: 0 ~ 60ºC) L, 25 mmL
Dimensions, weight 300(W) x 470(D) x 150(H) mm, 14 kg Spectrum measurement 200 ~ 460 nm
Power requirement AC 100 ~ 240 V, 50/60 Hz, 80 VA Dimensions, weight 300 (W) x 470 (D) x 230 (H) mm, 21 kg
Power requirement AC 100 ~ 240V, 50/60 Hz, 210 VA

15
Autosamplers
Model AS-4050/AS-4050i AS-4150 AS-4250 AS-4058
Full or partially fill loop injection (zero Full or partially fill loop injection (zero
Sample injection Full or partially fill loop injection (zero sample loss available), direct line sample loss available), direct line Full or partially fill loop injection (zero
method sample loss available) injection (option, change of flow-line injection (option, change of flow-line sample loss available)
is required.) is required.)
Number of samples 60 (2 mL vial) 180 (2 mL vial) 180 (2 mL vial) 40 (20 mL vial, standard)
0.1 ~ 100 µL 0.1 ~ 100 µL 0.1 ~ 100 µL
Injection volume (large volume injection kit: 1 ~ 1000 µ (large volume injection kit: 1 ~ 1000 µ (large volume injection kit: 1 ~ 1000 µ 1 ~ 10000 µL
L, option) L, option) L, option)
RSD 1.0 % or less (2500 µL, zero
RSD 0.3 % or less (specified RSD 0.25% or less (specified RSD 0.25% or less (specified
Injection precision sample loss)
conditions) conditions) conditions)
(specified conditions)
+/- 0.1% or less (with correction +/- 0.1% or less (with correction +/- 0.1% or less (with correction +/- 0.1% or less (with correction
Injection accuracy
function) function) function) function)
0.05% or less
0.01% or less 0.01% or less
(2000 µL, zero sample loss)
0.01% or less (2 µL) (specified conditions) (2 µL) (specified conditions)
Carry over (specified conditions)
(10 µL, specified conditions) 0.005% or less (with multiple solvent 0.005% or less (with multiple solvent
0.02% or less (with multiple solvent
flushing valve) (specified conditions) flushing valve) (specified conditions)
flushing valve) (specified conditions)
Maximum usable
30 MPa (AS-4050), 34 MPa (AS-4050i) 70 MPa 130 MPa 41 MPa
pressure
Sample
(option) temp. range: 4 ~ 40ºC (option) temp. range: 4 ~ 40ºC (option) temp. range: 4 ~ 40ºC (option) temp. range: 4 ~ 40ºC
cooling/heating
Pre-column derivatization (up to two Pre-column derivatization (up to two
Pre-column
- reagents), dilution, other user reagents), dilution, other user -
derivatization, etc
programs programs
Dimensions, weight 300 (W) x 470 (D) x 300 (H) mm, 21 kg 300 (W) x 470 (D) x 385.5 (H) mm, 25 kg 300 (W) x 470 (D) x 385.5 (H) mm, 25 kg 300 (W) x 470 (D) x 385.5 (H) mm, 25 kg
Power requirement AC 100 ~ 240V, 50/60 Hz, 60 VA AC 100 ~ 240V, 50/60 Hz, 75 VA AC 100 ~ 240V, 50/60 Hz, 75 VA AC 100 ~ 240V, 50/60 Hz, 75 VA

Column Ovens
Model CO-4061 CO-4062 CO-4060 CO-4065 RO-4068
T emperature range Ambient -15ºC ~ 100ºC Ambient -15ºC ~ 100ºC Ambient -15ºC ~ 80ºC Ambient -15ºC ~ 90ºC Ambient +10ºC ~ 200ºC
Column
compartment 270(W) x 230(D) x 60(H) mm 260(W) x 25(D) x 105(H) mm 110(W) x 105(D) x 410(H) mm 260(W) x 120(D) x 410(H) mm 43(W) x 350(D) x 75(H) mm
dimensions
Safety features Heating/cooling power shut off when unusually high temperatures or solvent leaks are detected.
300(W) x 470(D) x 150(H) mm, 300(W) x 470(D) x 150(H) mm, 150(W) x 470(D) x 475(H) mm, 300(W) x 470(D) x 475(H) mm, 300(W) x 470(D) x 150(H) mm,
Dimensions, weight
10 kg 10 kg 16 kg 25 kg 12 kg
AC 100 ~ 240 V, 50/60 Hz, AC 100 ~ 240 V, 50/60 Hz, AC 100 ~ 240 V, 50/60 Hz, AC 100 ~ 240 V, 50/60 Hz, AC 100 ~ 240 V, 50/60 Hz,
Power consumption
160 VA 200 VA 350 VA 530 VA 325 VA

ChromNAV Ver. 2
Language English/Japanese, selectable
Windows OS Windows 7 Professional 32/64 bit
Windows 8.1 Professional 32/64 bit
Controllable hardware LC-4000 Series, X-LC 3000 Series, LC-2000 Series,
Maximum 4 systems control, Maximum 20 systems registration
System control Direct control of instrument by Direct Mode,
T ime programming by Control Method,
Spectrum measurement,
Prevention from unauthorized use by security key,
Analog signal input,
Up to 4 channel simultaneous input per one LC-Net II/ADC

JASCO INTERNATIONAL CO., LTD.

11-10, Myojin-cho 1-chome, Hachioji, Tokyo 192-0046, Japan
Tel: +81-42-649-3247 Fax: +81-42-649-3518 http://www.jascoint.co.jp/english/
Australia, China, Hong Kong, India, Indonesia, Korea, Malaysia, New Zealand,
Pakistan, Philippines, Russia, Singapore, Taiwan, Thailand
JASCO INCORPORATED
28600 Mary's Court, Easton, MD 21601, U.S.A
Tel: +1-800-333-5272 +1-410-822-1220 Fax: +1-410-822-7526 http://www.jascoinc.com
U.S.A., Canada, Costa Rica, Mexico, Puerto Rico, Argentina, Brazil, Chile, Colombia, Paraguay,
Peru, Uruguay, Guatemala, , Ecuador, Bolivia
JASCO EUROPE s.r.l.
Via Luigi Cadorna 1, 23894 Cremella (Lc), Italy
Tel: +39-039-9215811 Fax: +39-039-9215835 http://www.jasco-europe.com
JASCO Deutschland www.jasco.de, JASCO UK www.jasco.co.uk,
JASCO France www.jascofrance.fr, JASCO Benelux www.jasco.nl,
JASCO Spain www.jasco-spain.com
Italy, Germany, U.K., France, Netherlands, Belgium, Luxembourg, Spain, Sweden, Norway,
Denmark,
Austria, Finland, Greece, Hungary, Poland, Portugal, Romania, Switzerland, Algeria, Cyprus,
Egypt, Israel, Jordan, Kuwait, Lebanon, Morocco, Saudi Arabia, South Africa, Tunisia,
Turkey, U.A.E., Yemen