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Genetic Engineering and DNA Manipulation

1. The document discusses genetic engineering techniques like recombinant DNA technology. It involves cutting donor DNA and plasmid DNA with restriction enzymes, and ligating the DNA fragments to form recombinant molecules which are then inserted into host cells. 2. The questions cover various stages of genetic engineering - cutting DNA, isolating DNA fragments, ligating DNA, transforming host cells, and expressing the new genes by producing proteins. 3. The document also contains questions about biological concepts like species, mechanisms of speciation, Darwin's theory of evolution, and homologous and analogous structures in taxonomy.
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0% found this document useful (0 votes)
74 views4 pages

Genetic Engineering and DNA Manipulation

1. The document discusses genetic engineering techniques like recombinant DNA technology. It involves cutting donor DNA and plasmid DNA with restriction enzymes, and ligating the DNA fragments to form recombinant molecules which are then inserted into host cells. 2. The questions cover various stages of genetic engineering - cutting DNA, isolating DNA fragments, ligating DNA, transforming host cells, and expressing the new genes by producing proteins. 3. The document also contains questions about biological concepts like species, mechanisms of speciation, Darwin's theory of evolution, and homologous and analogous structures in taxonomy.
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOCX, PDF, TXT or read online on Scribd

1. The ability to combine the DNA of one organism with the DNA of another organism.

A. Recombinant DNA
B. GMO
C. GEO
D. Ligation and Insertion
2. What is used to cut donor DNA and plasmid DNA?
A. Restriction site
B. Restriction enzymes
C. Splicing
D. Electrophoresis
3. In genetic engineering human genes can be inserted into a bacterium.
A. True B. False
4. Tiny segments of a gene are taken out and replaced by different genes.
A. Restriction enzyme
B. Recombinant DNA
C. Altered DNA
D. Gene splicing
5. Arrange the following in correspond to the correct sequence of genetic engineering.
1. Ligation and Insertion 2. Isolation 3. Transformation 4. Expression 5. Cutting
A. 1,2,3,4,5 B. 5,4,3,2,1
C. 2,1,5,4,3
D. 2,5,1,3,4
6. What stage in the formation of recombinant DNA is involved when cloning cells are
opened using chemicals and enzymes, donor DNA is extracted, and genetic probe is
added?
A. Isolation
B. Cutting
C. Insertion and Ligation
D. Expression
7. _______________ is the rejoining of cut fragments of DNA and forming artificial
recombinant molecules.
A. Ligation and Insertion
B. Isolation
C. Transformation
D. Expression
8. Genetic engineering means that DNA from different organisms can be combined.
A. True
B. False
9. Expression can be seen in which of the following? Select all that apply.
A. When the protein is produced in large amounts and it is isolated and purified.
B. When the organism with the recombinant DNA to produce the desired protein.
C. When the bacterial cell reproduces by binary fission.
D. When the plasmid will produce the polypeptide coded for by the donor DNA.
10. What is the different between transformation and expression?
A. Transformation is when recombinant DNA introduced into bacterial cell while
expression is when bacterial cell produces the polypeptide.
B. Transformation is when there is a rejoining of cut fragments and expression is when
there is a formation of artificial recombinant molecule.
C. Transformation is when the donor DNA is cut, and expression is when it is
connected to the host DNA.
D. All of the above
1. Which of the following statements about biological species is(are) correct?
I. Biological species is a group of individuals whose members interbreed with one
another.
II. Biological species are the model used for grouping extinct forms of life.
III. Members of biological species produce viable, fertile offsprings.
A. I only
C. I and III
E. I, II, and III
B. II only
D. II and III
2. The following isolating mechanisms prevent fertilization and formation of zygote except
A. Temporal isolation
C. Gametic isolation
E. Behavioral isolation
B. Hybrid breakdown
D. Ecological isolation
For numbers 3-5, use the following choices:
A. Allopatric speciation
B. Sympatric speciation
C. Parapatric speciation
B.3. Occurrence of abrupt genetic change cause reproductive isolation between groups of
individuals.
A.4. Occurs when populations are separated by a geographic barrier.
C.5. Abrupt change in the environment over a geographic border and strong disruptive selection
affects gene flow between neighboring populations
1. Catastrophism, meaning the regular occurrence of geological or meteorological
disturbances (catastrophes), was Cuvier's attempt to explain the existence of
A. Evolution.
B. The fossil record.
C. Uniformitarianism.
D. The origin of new species.
E. Natural selection.
2. Which of the following represents an idea that Darwin learned from the writings of
Thomas Malthus?
A. Technological innovation in agricultural practices will permit exponential
growth of the human population into the foreseeable future.
B. Populations tend to increase at a faster rate than their food supply normally
allows.
C. Earth changed over the years through a series of catastrophic upheavals.
D. The environment is responsible for natural selection.
E. Earth is more than 10,000 years old
3. In the mid-1900s, the Soviet geneticist Lysenko believed that his winter wheat
plants, exposed to ever-colder temperatures, would eventually give rise to ever more
cold tolerant winter wheat. Lysenko's attempts in this regard were most in agreement
with the ideas of
A. Cuvier.
B. Hutton.
C. Lamarck.
D. Darwin.
E. Lyell.
4. Which of the following ideas is not included in Darwin’s theory?
A. All organisms that have ever existed arose through evolutionary
modifications of ancestral species.
B. The great variety of species live today resulted from the diversification of
ancestral species.
C. Natural selection drives some evolutionary change.
D. Natural selection preserves favorable traits.
E. Natural selection eliminates adaptive traits.
5. Which of the following statements is not compatible with Darwin’s theory?
A. All organisms have arisen by descent with modification.
B. Evolution has altered and diversified ancestral species.
C. Evolution occurs in individuals rather than in groups.
D. Natural selection eliminates unsuccessful variations.
E. Evolution occurs in because some individuals function better than others in
a particular environment.
6. Which of the following must exist in a population before natural selection can act
upon that population?
A. Genetic variation among individuals
B. Variation among individuals caused by environmental factors.
C. Sexual reproduction
D. Three of the responses are correct.
E. Two of the responses are correct.
7. Which of the following does not contribute to the study of evolution?
A. Population genetics
B. Inheritance of acquired characteristics.
C. Fossil records
D. Comparative embryology
E. Comparative morphology
HOMOLOGOUS 1. forelimb of a bear, the wing of a bird, and human arm
PARALLELISM 2. South American and African monkeys
CONVERGENCE 3. North American wolves and Tasmanian wolves (thylacines)
ANALOGOUS 4. wings of a bird and a butterfly
ANALOGOUS 5. forelimb of a horse, the wing of a bird
ANALOGOUS 6. Penguins and fish both have fins.
ANALOGOUS 7. complex eyes of vertebrates, cephalopods (squid and octopus)
ANALOGOUS 8. shells of brachiopods and bivalve mollusks
HOMOLOGOUS 9. a true anteater (genus Myrmecophaga) and a marsupial anteater, or numbat
(Myrmecobius)
CONVERGENCE 10. tapirs and pigs
1. F Wings
2.C 6 Legs
3.A Segmented Body
4. G Double set of wings
5. G Cerci (abdominal appendages)
6.D Crushing mouthparts
7. B Legs
8. H. Curly Antennae

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