Product Guide 2023/24

reate a better w
c orl
to d
ab

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l
the

tsi
de
scientists inside
ling
ab

En

Our services and support help our

customers do what they do every day.
From drug discovery and
pharmaceutical development to food
safety and environmental analysis,
Phenomenex chromatography
solutions accelerate science and help
researchers improve global health and
well-being.
We are inspired by the impact our
customers make on the world and we
will always go above and beyond to
help them.

© 2023
2022 Phenomenex, Inc. All rights reserved.
 2–4

Table of 5–6

Contents
7 – 16
OUR COMPANY

REFERENCE STANDARDS
17 – 26
FILTRATION

SYRINGES
27 – 48

VIALS

SAMPLE PREPARATION / SPE 49 – 86

GC COLUMNS, ACCESSORIES,
AND APPLICATIONS
87 – 185
HPLC/UHPLC

SUPERCRITICAL FLUID
CHROMATOGRAPHY (SFC) 186 – 362

FLASH CHROMATOGRAPHY

363 – 372
PREP COLUMNS
AND BULK MEDIA

PURIFICATION AND ANALYSIS

OF SYNTHETIC DNA/RNA 373 – 382

ACCESSORIES
AND LAB SAFETY
383 – 402
APPENDICES

403 – 413

414 – 432

433 – 464
 Our Mission
Keeping scientists at the heart of our every day
through unforgettable support and standout
technology to build a brighter future together.

Our Promise
Our mission drives us to build sincere and
meaningful relationships by working alongside
scientists and connecting labs with exceptional
technology, unrivaled service, and world-class
technical support.
The Phenomenex team is empowered by a
culture built on trust, integrity, and kindness
— one that places scientists at the heart of
our every day. We are inspired by the impact
our customers make on the world and we will
always go above and beyond to help them in
our shared pursuit to create a brighter future for
humanity. That is our promise.

Matt Turner
President

2 Phenomenex
 Certified Reference Standards
Phenova™ Certified Reference Materials

3-6

AC C RE D ITE D

Reference Material Producer

Chemical Testing Laboratory
Certificate No. 2427.03
Certificate No. 2427.02

ACCR EDITED

“
Chemical
CertificateTesting
No. 2427.03Laboratory
Chemical Testing Laboratory

Certificate No. 2427.03

YOUR CUSTOMER SERVICE IS

AMAZING! The little things make a big
difference. I love the DOC reports! This
makes it so simple to manage our continued

”
performance documentation.

Certified Reference Standards

Phenova Certified Reference Materials..................................... 4
ARKANSAS ANALYTICAL INC.
The opinions stated herein are solely those of the speaker
and not necessarily those of any company or organization.
 Certified Reference Standards Certified Analytical Standards

Product available only in USA and Canada

Certified Reference Materials for Organic Environmental Analysis

Calibration, Internal, and Surrogate Standards
PHENOVA | CERTIFIED REFERENCE STANDARDS

PFAS CRMs Ordering Information

Product​ Part No. Qty. ​ Conc.
EPA 533 mix​ AL0-101838​ 1  mL​​ 2 µg / mL in methanol​
EPA 537.1 mix​ AL0-101839​ 1  mL​​ 2 µg / mL in methanol​
EPA 533 + 537.1 mix​ AL0-101840​ 1  mL​​ 2 µg / mL in methanol

PFAS CRMs List of Analytes Part No.: AL0-101939 AL0-101838 AL0-101840

CAS RN​ EPA 537.1 EPA 533​ EPA 533 + 537.1​
Perfluoroalkyl carboxylic acid (PFCA)​
Perfluorobutanoic acid (PFBA)​ 375-22-4​ NA​​​ ​
Perfluoropentanoic acid (PFPeA)​ 2706-90-3​ NA​​
Perfluorohexanoic acid (PFHxA)​ 307-24-4​
Perfluoroheptanoic acid (PFHpA)​ 375-85-9​
Perfluorooctanoic acid (PFOA)​ 335-67-1​
Perfluorononanoic acid (PFNA​)​ 375-95-1​
Perfluorodecanoic acid (PFDA)​ 335-76-2​
Perfluoroundecanoic acid (PFUnA)​ 2058-94-8​
Perfluorododecanoic acid (PFDoA)​ 307-55-1​
Perfluorotridecanoic acid (PFTrDA)​ 72629-94-8​ NA​​
Perfluorotetradecanoic acid (PFTeDA)​ 376-06-7​ NA​​
Perfluoroalkane sulfonic acid (PFSA)​​
Perfluorobutanesulfonic acid (PFBS)​ 375-73-5​
Perfluoropentanesulfonic acid (PFPeS)​ 2706-91-4​ NA​​
Perfluorohexanesulfonic acid - br/lin (PFHxS)​ 355-46-4​
Perfluoroheptanesulfonic acid (PFHpS)​ 375-92-8​ NA​​
Perfluorooctanesulfonic acid - br/lin (PFOS)​ 1763-23-1​
Perfluoroalkane sulfonamides (FASA) and derivatives​​
N-methyl perfluorooctanesulfonamidoacetic acid - br/lin (NMeFOSAA)​ 2355-31-9​ NA​​
N-ethyl perfluorooctanesulfonamidoacetic acid - br/lin (NEtFOSAA)​ 2991-50-6​ NA​​
Fluorotelomer sulfonic acid (FTSA)​​
4:2 Fluorotelomer sulfonic acid (4:2 FTS)​ 757124-72-4​ NA​​
6:2 Fluorotelomer sulfonic acid (6:2 FTS)​ 27619-97-2​ NA​​
8:2 Fluorotelomer sulfonic acid (8:2 FTS)​ 39108-34-4​ NA​​
Perfluoroalkyl ether carboxylic acid (PFECA)​​
Perfluoro-3-methoxypropanoic acid (PFMPA)​ 377-73-1​ NA​​
Perfluoro-4-methoxybutanoic acid (PFMBA)​ 863090-89-5​ NA​​
Hexafluoropropylene oxide dimer acid (HFPO-DA/GenX)​ 13252-13-6​
Nonafluoro-3,6-dioxaheptanoic acid (NFDHA)​ 151772-58-6​ NA​​
4,8-dioxa-3H-perfluorononanoic acid (ADONA​)​ 919005-14-4​
Polyfluoroalkyl ether sulfonic acid (PFESA)​​
Perfluoro(2-ethoxyethane)sulfonic acid (PFEESA)​ 113507-82-7​ NA​​
9-Chlorohexadecafluoro-3-oxanone-1-sulfonic acid (9Cl-PF3ONS)​ 756426-58-1​
11-chloroeicosafluoro-3-oxaundecane-1-sulfonic acid (11Cl-PF3OUdS)​ 763051-92-9​

4 Phenomenex
Certified Reference Standards Certified Analytical Standards

Ordering Information
Part No. Description Part No. Description
Polychlorinated Biphenyls (PCBs) Pesticides/Herbicides (cont’d)
AL0-101263 2,4,5,6-Tetrachloro-m-xylene 200 µg/mL in Acetone AL0-101781 4,4’-DDT Solution 100 µg/mL in Isooctane
AL0-101273 Aroclor 1016/1260 Standard 100 µg/mL in Hexane AL0-101782 Endrin Solution 100 µg/mL in Methanol
AL0-101274 Decachlorobiphenyl Standard 200 µg/mL in Acetone AL0-101784 Triphenylphosphate Solution 5000 µg/mL in MTBE
AL0-101275 Aroclor 1016 Standard 100 µg/mL in Hexane AL0-101785 o,p Chlorinated Pesticides 250 µg/mL in Isooctane
AL0-101276 Aroclor 1221 Standard 100 µg/mL in Hexane AL0-101786 DCPA Diacid Mix 100 µg/mL in Methanol

PHENOVA | CERTIFIED REFERENCE STANDARDS

AL0-101277 Aroclor 1232 Standard 100 µg/mL in Hexane AL0-101792 100 ppm Tech Chlordane Mix 100 µg/mL in Hexane
AL0-101278 Aroclor 1242 Standard 100 µg/mL in Hexane AL0-101793 50 ppm Endothall Mix 50 µg/mL in Methanol
AL0-101279 Aroclor 1248 Standard 100 µg/mL in Hexane AL0-101812 UCMR 5 525.3 Pesticides Mix 1000 µg/mL in Methanol
AL0-101280 Aroclor 1254 Standard 100 µg/mL in Hexane AL0-101813 UCMR 5 527 Pesticides Mix 1000 µg/mL in Methanol
AL0-101281 Aroclor 1260 Standard 100 µg/mL in Hexane AL0-101814 UCMR 5 531.2 Herbs Mix 1000 µg/mL in Methanol
AL0-101282 Aroclor 1262 Standard 100 µg/mL in Hexane AL0-101815 UCMR 5 538 Pesticide Mix 1000 µg/mL in Methanol
AL0-101283 Aroclor 1268 Standard 100 µg/mL in Hexane AL0-101831 538 Internal Standard 1000 µg/mL in Methanol
AL0-101455 Aroclor 1016/1260 1000 µg/mL in Hexane AL0-101834 556 Internal Standard 10000 µg/mL in Hexane
AL0-101456 Aroclor 1016 1000 µg/mL in Hexane AL0-101835 556 Surrogate Mix 10000 µg/mL in Acetonitrile
AL0-101457 Aroclor 1221 1000 µg/mL in Hexane AL0-101424 Dinoseb 1000 µg/mL in Methanol
AL0-101458 Aroclor 1232 1000 µg/mL in Hexane AL0-101644 Glyphosate Standard 1000 µg/mL in Water
AL0-101459 Aroclor 1242 1000 µg/mL in Hexane AL0-101645 Diquat/Paraquat Mix 1000 µg/mL in Water
AL0-101460 Aroclor 1248 1000 µg/mL in Hexane AL0-101646 Endothal Standard 1000 µg/mL in Acetone
AL0-101461 Aroclor 1254 1000 µg/mL in Hexane Semi-Volatiles
AL0-101462 Aroclor 1260 1000 µg/mL in Hexane AL0-101233 PAH Plus Methylnaphthalenes 2000 µg/mL Methylene Chloride
AL0-101463 Aroclor 1262 1000 µg/mL in Hexane AL0-101234 8270 Phenols Standard 2000 µg/mL Methylene Chloride
AL0-101464 Aroclor 1268 1000 µg/mL in Hexane AL0-101235 Appendix IX Mix 1 2000 µg/mL Methylene Chloride
AL0-101466 Aroclor 1016/1260 1000 µg/mL in Isooctane AL0-101236 PAH Standard 2000 µg/mL Methylene Chloride
AL0-101467 Aroclor 1016 1000 µg/mL in Isooctane AL0-101238 SV Internal Standard Mix 4000 µg/mL Methylene Chloride
AL0-101468 Aroclor 1221 1000 µg/mL in Isooctane AL0-101239 SV B/N Surrogate Mix 2000 µg/mL Methylene Chloride
AL0-101469 Aroclor 1232 1000 µg/mL in Isooctane AL0-101240 SV B/N Surrogate Mix 5000 µg/mL Methylene Chloride
AL0-101470 Aroclor 1242 1000 µg/mL in Isooctane AL0-101241 SV Acids Surrogate Mix 2000 µg/mL in Methanol
AL0-101471 Aroclor 1248 1000 µg/mL in Isooctane AL0-101242 SV Acids Surrogate Mix 5000 µg/mL in Methanol
AL0-101472 Aroclor 1254 1000 µg/mL in Isooctane AL0-101243 8270 Benzidines Standard 2000 µg/mL Methylene Chloride
AL0-101473 Aroclor 1260 1000 µg/mL in Isooctane AL0-101244 Benzidines Standard 2000 µg/mL Methylene Chloride
AL0-101474 Aroclor 1262 1000 µg/mL in Isooctane AL0-101245 N-Nitrosodiphenylamine 1000 µg/mL Methylene Chloride
AL0-101475 Aroclor 1268 1000 µg/mL in Isooctane AL0-101246 Benzoic Acid 2000 µg/mL Methylene Chloride
AL0-101702 2,4,5,6-Tetrachloro-m-xylene 200 µg/mL in Acetone; 5mL AL0-101251 SV BNA Surrogate Mix 2000 µg/mL Methylene Chloride
AL0-101703 Decachlorobiphenyl (BZ #209) 200 µg/mL in Acetone; 5mL AL0-101252 SV BNA Surrogate Mix 4000 µg/mL Methylene Chloride
Pesticides/Herbicides AL0-101291 GC/MS Tuning Mix 1000 µg/mL in Methylene Chloride
AL0-101253 8081 Chlorinated Pesticides 1000 µg/mL Hexane/Toluene AL0-101305 1,2-Dichlorobenzene-d4 2000 µg/mL in P&T Methanol
AL0-101254 Chlorinated Pesticides Std 2000 µg/mL Hexane/Toluene AL0-101401 Phenanthrene-d10 2000 µg/mL in MeCl2
AL0-101255 8141 OP Pesticides 200 µg/mL Hexane/Acetone 90:10 AL0-101404 p-Terphenyl-d14 1000 µg/mL in MeCl2
AL0-101256 8270 OP Pesticides Mix 2000 µg/mL Methylene Chloride AL0-101443 Anline 1000 µg/mL Methylene Chloride
AL0-101257 Pesticide Surrogate Mix 200 µg/mL in Acetone AL0-101444 8270 Calibration Standard 1000 µg/mL MeCL2/MeOH
AL0-101258 Toxaphene 2000 µg/mL in Methanol AL0-101447 SV Acids Surrogate Mix 10,000 µg/mL in Methanol
AL0-101260 Technical Chlordane 1000 µg/mL in Methanol AL0-101448 SV Acids Surrogate Mix 10,000 µg/mL in Methanol
AL0-101261 2,4-Dichlorophenylacetic Acid 1000 µg/mL in Acetone AL0-101450 SV B/N Surrogate Mix 5000 µg/mL Methylene Chloride
AL0-101266 Triazine Herbicides Mix 200 µg/mL in Acetone AL0-101451 SV B/N Surrogate Mix; 10 mL 5000 µg/mL Methylene Chloride
AL0-101288 Chlorinated Acid Herbicides 100 µg/mL in Acetonitrile AL0-101454 Decafluorotriphenylphosphine 2500 µg/mL Methylene Chloride
AL0-101289 MCPP & MCPA 20000 µg/mL in Acetonitrile AL0-101491 Appendix IX Mix 3 1000 µg/mL in Methylene Chlor
AL0-101452 Pesticide Surrogate Mix, 5 mL 200 ug/mL in Acetone AL0-101492 8270 Matrix Spike
AL0-101515 508.1 Performance Check Mix 0.02-0.5 ug/ml in Ethyl Acetat AL0-101493 Acid Matrix Spike Mix 2000 µg/mL in Methanol
AL0-101530 531.1 Carbamates Mix 100 µg/mL in Methanol AL0-101494 Acid Matrix Spike Mix 10000 µg/mL in Methanol
AL0-101541 8141 OP Pesticides Mix 2 200 µg/mL each in Hex/Ace 9:1 AL0-101495 Acid Matrix Spike Mix 10000 µg/mL in Methanol
AL0-101566 Technical Chlordane Standard 1000 µg/mL in Hexane AL0-101496 8310 PAH Standard 500 µg/mL in Acn/Tol (92:8)
AL0-101625 525.3 Pesticides Standard 1000 µg/mL in Methanol AL0-101497 8310 QC Check Standard 5-100 µg/mL in Acetonitrile
AL0-101647 4,4’-DDT/Endrin Mix 200 µg/mL in Hexane AL0-101498 8310 Surrogate Standard 1000 µg/mL in Acetonitrile
AL0-101657 505 Organohalide Pesticide Mix 200 µg/mL in Methanol AL0-101500 604 Phenols Standard 2000 µg/mL in Methanol
AL0-101658 525.2 Organonitrogen Pesticide 500 µg/mL in Acetone AL0-101501 605 Benzidines Standard 2000 µg/mL in Methanol
AL0-101665 Toxaphene Standard 1000 µg/mL in Hexane AL0-101505 610 PAH Mix 2 100 - 2000 µg/mL in MeCl2
AL0-101668 Free Acids Herbicides Mix 200 µg/mL in Methanol AL0-101509 625 Calibration Standard 1000 µg/mL in MeCL2/MEOH (98:2
AL0-101669 Methyl Ester Herbicide Mix # 1 200 µg/mL in Hexane AL0-101524 525.2 GC-MS Performance Check 1000 µg/mL in Acetone
AL0-101670 Methyl Ester Herbicide Mix # 2 200 µg/mL in Hexane/MTBE AL0-101525 EPA 525.2 Semivolatile Mix 1000 µg/mL in Acetone
AL0-101671 Dalapon Methyl Ester 2000 µg/mL in Hexane AL0-101526 525.2 Internal Standard 1000 µg/mL in Acetone
AL0-101672 DCAA Methyl Ester 200 µg/mL in Hexane AL0-101527 525.2 Surrogate Standard 1000 µg/mL in Acetone
AL0-101675 MCPA/MCPP Methyl Ester Mix 20000 µg/mL in Hexane AL0-101532 1,2,3-Trichloropropane 1000 µg/mL in MTBE
AL0-101682 Pesticides Matrix Spike Mix 50-100 µg/mL in Methanol AL0-101533 2,3-Dibromopropionic acid 1000 µg/mL in MTBE
AL0-101704 BDMC 100 µg/mL in Methanol AL0-101534 Haloacetic Acid Standad 1000 µg/mL in MTBE
AL0-101705 Tributylphosphate 1000 µg/mL in Acetone AL0-101535 552 Haloacetic Acid Standard 1000 µg/mL in MTBE
AL0-101723 529 IS Mix 1000 µg/mL in Ethyl Acetate AL0-101536 3-Methylcholanthrene 2000 µg/mL in MeCl2
AL0-101724 529 Surrogate Fortification Mi 100 µg/mL in Methanol AL0-101538 B/N Matrix Spike Mix 1000 µg/mL in Methanol
AL0-101775 632 Carbamates/Urea Mix 1000 µg/mL in Acetonitrile AL0-101539 B/N Matrix Spike Mix 5000 µg/mL in Methanol
AL0-101776 8151 Herbicides Mix 100- 10000 µg/mL in Methanol AL0-101540 B/N Matrix Spike Mix 5000 µg/mL in Methanol

Phenomenex 5
 Certified Reference Standards Certified Analytical Standards

Part No. Description Part No. Description

Semi-Volatiles (cont’d) Volatile Organic Analysis (VOA) (cont’d)
AL0-101548 BN Extractables Standard 500 µg/mL in MeCL2 AL0-101218 524 Surrogate Standard Mix 2 2000 µg/mL in P&T Methanol
AL0-101551 100 µg/mL Caffeine Standard 100 µg/mL in Methanol AL0-101219 504.1 Calibration Standard 200 µg/mL in P&T Methanol
AL0-101552 300 µg/mL Caffeine Standard 300 µg/mL in Methanol AL0-101220 524 Surrogate Standard Mix 1 2000 µg/mL in P&T Methanol
AL0-101553 500 µg/mL Caffeine Standard 500 µg/mL in Methanol AL0-101221 502.2 Internal Standard Mix 1000 µg/mL in P&T Methanol
AL0-101554 1000 µg/mL Caffeine Standard 1000 µg/mL in Methanol AL0-101222 Acrolein/Acrylonitrile 1000 µg/mL in P&T Methanol
PHENOVA | CERTIFIED REFERENCE STANDARDS

AL0-101556 p-Terphenyl 10000 µg/mL in MeCl2 AL0-101223 Acrolein 1000 µg/mL in P&T Methanol
AL0-101561 Benzo(a)pyrene-d12 Standard 1000 µg/mL in MeCl2 AL0-101224 Acrolein 5000 µg/mL in Water
AL0-101567 B/N Surrogate Mix 2 2000 µg/mL in MeCl2 AL0-101225 1-Chloro-2-fluorobenzene 1000 µg/mL in P&T Methanol
AL0-101568 Acids Surrogate Mix 2 2000 µg/mL in Methanol AL0-101226 4-Bromofluorobenzene 1000 µg/mL in P&T Methanol
AL0-101569 B/N Surrogate Mix 2 5000 µg/mL in MeCl2 AL0-101227 2-Chloroethyl vinyl ether 2000 µg/mL in P&T Methanol
AL0-101570 Acids Surrogate Mix 2 5000 µg/mL in Methanol AL0-101228 Vinyl acetate 2000 µg/mL in Water
AL0-101618 SV Internal Standard Mix 4000 µg/mL Methylene Chloride AL0-101229 Fluorobenzene 2000 µg/mL in P&T Methanol
AL0-101626 EPA 530 Standard 1000 µg/mL in Methanol AL0-101230 1-Chloro-4-fluorobenzene 2000 µg/mL in P&T Methanol
AL0-101627 EPA 530 Surrogate Mix 1000 µg/mL in Methanol AL0-101231 4-Bromofluorobenzene 2000 µg/mL in P&T Methanol
AL0-101628 EPA 530 IS Mix 1000 µg/mL in Methanol AL0-101353 Naphthalene-d8 2000 µg/mL in MeCl2
AL0-101637 Fluoranthene-d10 2000 µg/mL in MeCl2 AL0-101362 Toluene-d8
AL0-101638 2-Methylnaphthalene-d10 2000 µg/mL in MeCl2 AL0-101445 8260 Internal Standard Mix 1 2500 µg/mL in P&T MeOH 5mL
AL0-101639 PAH SIM Surogate Mix 2000 µg/mL in MeCl2 AL0-101446 8260 Surrogate Standard 1 2500 µg/mL in P&T Methanol 5mL
AL0-101652 TCLP BNA Mix 2000 µg/mL in MeCl2 AL0-101465 504.1 Calibration Standard 2000 µg/mL in P&T Methanol
AL0-101655 1,4-Dioxane Standard 2000 µg/mL in MeCl2 AL0-101476 2-Methylnaphthalene 2000 µg/mL in P&T Methanol
AL0-101659 612 Chlorinated Hydrocarbons 2000 µg/mL in Isooctane AL0-101478 8010A Calibration Mix 1 2000 µg/mL in P&T Methanol
AL0-101662 Acenaphthene-d10 2000 µg/mL in MeCl2 AL0-101479 8010A Calibration Mix 2 2000 µg/mL in P&T Methanol
AL0-101673 2,4-Dinitrophenol 1000 µg/mL in Methanol AL0-101480 8020A Calibration Standard 2000 µg/mL in P&T Methanol
AL0-101674 Acids Surrogate Mix 2, 5mL 7500 µg/mL in Methanol AL0-101481 8021 Surrogate Standard 1500 µg/mL in P&T Methanol
AL0-101676 PAH Supplement Mix 1000 µg/mL in MeCl2 AL0-101486 8240/8260 Performance Check 2000 µg/mL in P&T Methanol
AL0-101712 Diphenylamine 5000 µg/mL in Methanol AL0-101487 8260B Calibration Standard 2000 µg/mL in P&T Methanol
AL0-101720 Nitroglycerin Standard 1000 µg/mL in Acetonitrile AL0-101488 8260 Matrix Spike Mix 2500 µg/mL in P&T Methanol
AL0-101721 PETN Standard 1000 µg/mL in Acetonitrile AL0-101489 tert-Amyl ethyl ether 2000 µg/mL in P&T Methanol
AL0-101722 Tetryl Standard 1000 µg/mL in Acetonitrile AL0-101490 a, a, a-Trifluorotoluene 2000 µg/mL in P&T Methanol
AL0-101726 8330 IS Mix 1000 µg/mL in Acetonitrile AL0-101499 602 Purgable Aromatics 2000 µg/mL in P&T Methanol
AL0-101743 Perylene-d12 1000 µg/mL in MeCl2 AL0-101506 624 Internal Standard 1500 µg/mL in P&T Methanol
AL0-101755 1,4-Dioxane 1000 µg/mL in MeCl2 AL0-101507 624 Calibration Standard 2000 µg/mL in P&T Methanol
AL0-101759 Nitroglycerin Standard 1000 µg/mL in Methanol AL0-101508 624 Surrogate Standard 2000 µg/mL in P&T Methanol
AL0-101760 PETN Standard 1000 µg/mL in Methanol AL0-101519 524 IS/Surrogate Mix 2000 µg/mL in P&T Methanol
AL0-101761 8330B Nitroaromatic& Nitramine AL0-101542 8260 IS/Surrogate Mix, 5 mL 5000 µg/mL in P&T Methanol
AL0-101787 521 Surrogate Standard 1000 µg/mL in MeCl2 AL0-101549 HAP Standard 10000 µg/mL in Water
AL0-101788 521 Internal Standard 1000 µg/mL in MeCl2 AL0-101550 Methanol Standard 10000 µg/mL in Water
AL0-101789 521 Nitrosamines Mix 1000 µg/mL in MeCl2 AL0-101555 1,4-Dioxane-d8 2000 µg/mL in P&T Methanol
AL0-101816 UCMR 5 542 Pharmacuticals Mix 1000 µg/mL in Methanol AL0-101564 1,4-Dioxane Standard 10000 µg/mL in P&T Methanol
AL0-101817 UCMR 5 551.1 Mix 1000 µg/mL in MeOH/Acetone AL0-101571 Glycols Standard 50000 µg/mL in Water
AL0-101819 UCMR 5 558 Mix 1000 µg/mL in MeOH AL0-101573 Methanol Standard 2000 µg/mL in Water
AL0-101820 UCMR 5 559 Phenols Mix 1000 µg/mL in MeOH AL0-101592 Ethanol 40000 µg/mL in P&T Methanol
AL0-101830 525/527 Surrogate Mix 1000 µg/mL in Acetone AL0-101593 Isopropanol 20000 µg/mL in P&T Methanol
AL0-101832 542 Internal Standard 250-1000 µg/mL in Methanol AL0-101613 Perfluorotributylamine (PFTBA)
AL0-101833 542 Surrogate Mix 1000 µg/mL in Methanol AL0-101614 Perfluorotributylamine (PFTBA)
AL0-101836 559 Surrogate Mix 100 µg/mL in Methanol AL0-101617 Chloroprene 2000 µg/mL in P&T Methanol
AL0-101838 533 PFAS Calibration Standard 2 µg/mL in P&T Methanol AL0-101629 EPA 541 Standard 1000 µg/mL in P&T Methanol
AL0-101839 537 PFAS Calibration Standard 2 µg/mL in P&T Methanol AL0-101630 EPA 541 IS Mix 1000 µg/mL in P&T Methanol
AL0-101840 533/537 PFAS Calibration Standard AL0-101633 1% Ethanol Standard 10,000 µg/mL in Water
AL0-101650 Methapyrilene Standard 1000 µg/mL in MeCl2 AL0-101651 TCLP Volatiles Mix 1000 µg/mL in P&T Methanol
Volatile Organic Analysis (VOA) AL0-101660 EPA 524.2 Rev 4 Ketones Mix 2000 µg/mL in P&T MeOH/H20
AL0-101200 502.2 Calibration Standard 2000 µg/mL in P&T Methanol AL0-101661 EPA 524 Rev 4 Additions Mix 2000 µg/mL in P&T MeOH
AL0-101201 502.2 Calibration Standard 200 µg/mL in P&T Methanol AL0-101663 SOM01.1 Non-Ketone DMC Mix 500 µg/mL in Methanol-OD
AL0-101202 8260 Appendix IX Additions Mix AL0-101664 SOM01.1 Ketone DMC Mix 500 µg/mL in MeOH-OD/H2O
AL0-101203 BTEX Standard 2000 µg/mL in P&T Methanol AL0-101701 Ethanol 2000 µg/mL in P&T Methanol
AL0-101204 BTEX plus MTBE Standard 2000 µg/mL in P&T Methanol AL0-101711 Tetrahydrofuran-d8 2000 µg/mL in P&T Methanol
AL0-101205 BTEX plus MTBE Standard 200 µg/mL in P&T Methanol AL0-101713 601 Purgable Halocarbons Mix 2000 µg/mL in P&T Methanol
AL0-101206 VOA Gases Standard 2000 µg/mL in P&T Methanol AL0-101716 VOA Surrogate Mix 1000 µg/mL in P&T Methanol
AL0-101207 VOA Gases Standard 200 µg/mL in P&T Methanol AL0-101757 1,2,3-Trichloropropane-d5 IS M
AL0-101208 California Oxygenates Standard AL0-101758 8260 Acetates Standard 2000 µg/mL in P&T Methanol
AL0-101209 501 Trihalomethanes Standard 2000 µg/mL in P&T Methanol AL0-101794 OLC 3.2 VOA Calibration Mix 1000- 2000 µg/mL in P&T MeOH
AL0-101210 501 Trihalomethanes Standard 200 µg/mL in P&T Methanol AL0-101818 UCMR 5 556.1 Aldehydes Mix 1000 µg/mL in MeOH/H2O
AL0-101211 Ketones Standard
Accreditations
AL0-101212 Ketones Standard 5000 µg/mL P&T MeOH/H2O (90:10)
AL0-101213 8260 Internal Standard Mix 1 2500 µg/mL in P&T Methanol ISO 17034 ISO/IEC 17025:2005
Competence of reference material Competence of testing and calibration
AL0-101214 8260 Surrogate Standard 1 2500 µg/mL in P&T Methanol
producers. A2LA Cert No. 2427.02 laboratories. A2LA Cert No. 2427.03
AL0-101215 8260 Internal Standard Mix 2 2500 µg/mL in P&T Methanol
AL0-101216 8260 Surrogate Standard Mix 2 2500 µg/mL in P&T Methanol ISO/IEC 17043:2010 TNI EL-V3-2016
Conformity assessment. Proficiency testing. Environmental proficiency testing providers.
AL0-101217 524 Internal Standard Mix 1 2000 µg/mL in P&T Methanol
A2LA Cert No. 2427.01 A2LA Cert No. 2427.01

6 Phenomenex
 Filtration
Sample Handling and Mobile Phase

7 - 16

“
Filtration Products
Column Couplers ..................................................................... 16
Filter Membranes .................................................................... 13 We have found your syringe
Inlet Filters
filters to be competitively priced
and well made compared with our
Metal-free / Biocompatible.................................................. 14
usual brand. They don’t seem to
Stainless Steel...................................................................... 14
clog as quickly and there is no loss
In-Line Filters
of performance for our sample prep,
Biocompatible PEEK.....................................................15 - 16

”
usually 2mL vials of Pharmaceutical
Stainless Steel...............................................................15 - 16 active ingredients.
Mobile Phase Filtration Accessories................................11 - 12 Wes Herridge
Mobile Phase Filtration System............................................... 11 Laleham Healthcare, UK
Solvent Pickup Adapter........................................................... 12 The opinions stated herein are solely those of the speaker
and not necessarily those of any company or organization.
Solvent and Waste Protection................................................. 12
Syringe Filters
Non-Sterile.......................................................................8 - 10
Sterile.................................................................................... 10
Syringes, Disposable............................................................... 10
 Filtration Syringe Filters
Phenex LC/GC Approved Phenex Offers:

Syringe Filters
• Broad chemical compatibility • 100 % integrity tested
• Minimized extractables • Low hold-up volume
• Excellent flow rate • Low protein adsorption
For sample and solvent filtration prior to • High total throughput • Bi-directional use
LC or GC analysis • Certified quality

• Particulate, PVC, and extractable-free filters

• Less system downtime
• More consistent, reproducible results
FILTRATION - SAMPLE HANDLING

• Increased column lifetime

Syringe Filter Selection Guide

1. Choose filter diameter based on sample volume
≤ 2 mL 2 - 10 mL 10 - 100 mL
Sample Volume Sample Volume Sample Volume
|
SYRINGE FILTERS

4 mm Diameter 15 mm Diameter 25 - 28 mm Diameter

2. Choose a pore size based on your sample

Sample Description Recommended Filter Pore Size
General aqueous or mixed organic samples prior to LC analysis with columns packed with > 3 µm particles.
General clarification of GC, SFC, CE, and GPC samples. 0.45 µm
Viscous samples or samples containing high levels of particulate matter.
General aqueous or mixed organic samples prior to LC analysis with columns packed with ≤ 3 µm particles.
0.20 µm
Removal of fine particulate matter prior to GC, SFC, CE, and GPC samples.
Viscous samples such as serum, plasma or other biological matrices. Solutions with high particulate load such Glass Fiber Filter with 0.45 µm
as some environmental, biofuels or food and beverage applications. filter membrane

3. Suggested syringe filter membranes

Membrane Type Recommended Uses
RC For Aqueous and A broad range of aqueous and mixed-organic solutions.
(Regenerated Cellulose) Mixed Organic Fast-flow and ultra-low protein and non-specific binding characteristics.
Solutions Broadly recommended as an excellent general purpose/high-performance sample filter for most applications.
PTFE, Teflon® For 100% Well-suited for the clarification of non-aqueous samples.
(Polytetrafluoroethylene) Organic Solutions Hydrophobic membrane, excellent for filtration of organic-based, highly acidic or basic samples and solvents.
A hydrophobic membrane, that can be made hydrophilic by wetting with alcohol and then flushing with deionized water.

Additional syringe filter membranes

Membrane Type Recommended Uses
PES Polyethersulfone membranes exhibit very fast-flow and ultra-low protein binding characteristics.
(Polyethersulfone) Phenex-PES membranes are typically broadly recommended for filtering critical biological samples, tissue culture media,
additives and buffers.
NY Nylon has inherent hydrophilic characteristics and works well for filtration of many aqueous and mixed-organic samples.
(Nylon) In combination with a glass pre-filter (Phenex-GF/NY), this membrane is excellent for the filtration of particle-laden samples, such as foods and
beverages, environmental, biofuels, and dissolution samples.
CA Cellulose Acetate (CA) membranes exhibit ultra-low protein binding and are broadly used in the filtration of biological samples. In combination with a
(Cellulose Acetate) glass pre-filter (Phenex-GF/CA), this membrane is excellent for filtration of tissue culture media, general biological sample filtration and clarification.

GF Glass Fiber (GF) filters are made of inert borosilicate glass and have a nominal 1.2 μm pore size. They are commonly used with highly viscous
(Glass Fiber) samples or samples containing high concentrations of particulate matter (e.g., food analysis, biological samples, soil samples, fermentation broth
samples, removal of yeasts, molds, etc.).
PVDF Hydrophilic PVDF membrane provides high flow rates and throughput, low extractables, and broad chemical compatibility.
(Polyvinylidene Fluoride) This membrane binds less protein than nylon or PTFE membranes.

8 Phenomenex
Filtration Syringe Filters
Phenex™ Syringe Filters (cont’d)
Tip: Try a Sample Pack!
Request yours today by phone or visit
www.phenomenex.com/sample
Ordering Information 1
4 mm Diameter 15 mm Diameter 25 - 28 mm Diameter
Phenex Syringe Filters for ≤ 2 mL sample volumes for 2 – 10 mL sample volumes for 10 – 100 mL sample volumes
Membrane Type/Size Part No. Unit Part No. Unit Part No. Unit

RC AF0-3203-12 100/pk AF0-2203-12 100/pk AF0-8203-12 5

100/pk
(Regenerated Cellulose)

SAMPLE HANDLING - FILTRATION

AF0-3203-52 500/pk AF0-2203-52 500/pk AF0-8203-52 5
500/pk
PES 3 — — — — AF0-8208-12 7
100/pk
(Polyethersulfone)
— — — — AF0-8208-52 7
500/pk
PTFE 6 AF0-3202-12 100/pk AF0-2202-12 100/pk AF0-1202-12 100/pk
(Polytetrafluoroethylene)
AF0-3202-52 500/pk AF0-2202-52 500/pk AF0-1202-52 500/pk
NY AF3-3207-12 100/pk AF0-2207-12 100/pk AF0-1207-12 100/pk
(Nylon)
AF3-3207-52 500/pk AF0-2207-52 500/pk AF0-1207-52 500/pk
GF/NY 2 An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a Nylon AF0-1A47-12 7 100/pk
0.20 µm

(Glass Fiber/Nylon) (NY) membrane. Excellent for filtration of particle-laden samples, such as foods and beverages,
environmental, biofuels, and dissolution samples. Use less hand pressure to filter even the most AF0-1A47-52 7 500/pk
difficult samples. Outlet connection is luer lock.

|
PVDF — — AF6-5206-12 8 100/pk AF6-6206-12 100/pk

SYRINGE FILTERS
(Polyvinylidene Fluoride) — — AF6-5206-52 8 500/pk AF6-6206-52 500/pk
GF/PVDF An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a PVDF AF6-6C06-12 100/pk
(Glass Fiber/Polyvinylidene membrane. The hydrophilic PVDF membrane provides high flow rates and throughput, low
Fluoride) extractables and broad chemical compatibility. This membrane binds less protein than nylon or AF6-6C06-52 500/pk
PTFE membranes.
CA 4 — — — — AF0-8204-12 7
100/pk
(Cellulose Acetate) — — — — AF0-8204-52 7
500/pk
GF/CA 2,3,4 An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a CA AF0-8A09-12 7
100/pk
(Glass Fiber/Cellulose Acetate) membrane. Excellent for filtration of tissue culture media, general biological sample filtration
and clarification. Outlet connection is luer lock. AF0-8A09-52 7
500/pk

RC AF0-3103-12 100/pk AF0-2103-12 100/pk AF0-8103-12 5

100/pk
(Regenerated Cellulose)
AF0-3103-52 500/pk AF0-2103-52 500/pk AF0-8103-52 5
500/pk
PES 3 — — — — AF0-8108-12 7
100/pk
(Polyethersulfone)
— — — — AF0-8108-52 7
500/pk
PTFE 6 AF0-3102-12 100/pk AF0-2102-12 100/pk AF0-1102-12 100/pk
(Polytetrafluoroethylene)
AF0-3102-52 500/pk AF0-2102-52 500/pk AF0-1102-52 500/pk
NY AF3-3107-12 100/pk AF0-2107-12 100/pk AF0-1107-12 100/pk
(Nylon)
AF3-3107-52 500/pk AF0-2107-52 500/pk AF0-1107-52 500/pk
0.45 µm

GF/NY 2 An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a Nylon AF0-1B47-12 7 100/pk
(Glass Fiber/Nylon) (NY) membrane. Excellent for filtration of particle-laden samples, such as foods and beverages,
AF0-1B47-52 7 500/pk
environmental, biofuels, and dissolution samples. Use less hand pressure to filter even the most
difficult samples. Outlet connection is luer lock.
PVDF — — AF6-5106-12 8 100/pk AF6-6106-12 100/pk
(Polyvinylidene Fluoride) — — AF6-5106-52 8 500/pk AF6-6106-52 500/pk
GF/PVDF An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a PVDF
AF6-6D06-12 100/pk
(Glass Fiber/Polyvinylidene membrane. The hydrophilic PVDF membrane provides high flow rates and throughput, low
Fluoride) extractables and broad chemical compatibility. This membrane binds less protein than nylon or
AF6-6D06-52 500/pk
PTFE membranes.
GF/CA 2,3,4 An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a CA AF0-8B09-12 7
100/pk
(Glass Fiber/Cellulose Acetate) membrane. Excellent for filtration of tissue culture media, general biological sample filtration
AF0-8B09-52 7
500/pk
and clarification. Outlet connection is luer lock.
1.20 µm

GF 2,3 Prefiltration of heavily contaminated or highly viscous samples. When used in-series preced- AF0-8515-12 7
100/pk
(Glass Fiber) ing a membrane filter, clogging of the membrane filter is prevented and sample clean up is
AF0-8515-52 7
500/pk
optimized. Outlet connection is luer lock.
1. Larger quantity purchases at significant savings are available. 4. Cellulose acetate is surfactant-free. 7. 28 mm diameter.
2. Glass fiber filters are 28 mm diameter and made of borosilicate. 5. 26 mm diameter. 8. 17 mm diameter.
They will remove 90 % of all particles >1.2 µm. 6. Hydrophobic membrane. Can be made 9. Additional dimensions and membrane types are available.
3. Housing material is methacrylate butadiene styrene (MBS) polymerisate. hydrophilic by pre-wetting with IPA. Please contact your local Phenomenex technical consultant or
Also known as Cyrolite®. distributor for availability or assistance.

Above syringe filters are non-sterile. Housing is made of medical-grade polypropylene (PP),
and offer luer lock inlet/slip outlet connections, unless otherwise indicated.

Phenomenex 9
 Filtration Syringe Filters and Disposable Syringes
Phenex™ Syringe Filters (cont’d)

Syringe Filter Applications and Recommended Membranes

Recommended First Second
Application / Sample Filter Alternative Alternative
LC and GC Sample Prep RC PTFE PES
Aggressive or Pure Organic Solvents PTFE RC NY
Protein Analysis / Biological Samples PES RC GF/CA
High Particulate Loads GF/NY GF + RC PTFE
Environmental Methods GF/NY RC PTFE
| FILTRATION - SAMPLE HANDLING

Food and Beverage GF/NY RC PTFE

Clinical Research / Toxicology RC PES NY
Dissolution Testing GF/NY RC PTFE
Ion Chromatography RC PES PTFE
Trace Metals (ICP-MS, AAS) RC PES NY
Capillary Electrophoresis (CE) RC PES NY
Tissue Cultures, Media, Buffers GF/CA PES RC

For high load and particulate-laden samples you may consider

placing a Glass Fiber (GF) prefilter, either integrated with the
membrane as one unit (Phenex-GF/NY or -GF/CA) or in series
with the membrane syringe filter of your choice.

Syringe Filter Finder

DISPOSABLE SYRINGES / STERILE SYRINGE FILTERS / SYRINGE FILTERS

3-step tool designed to help you find the

appropriate syringe filter to help you successfully
remove particulates from your sample matrix.
www.phenomenex.com/SFfinder

Sterile Syringe Filters All-Plastic Disposable Syringes

Sterile syringe filters from Phenomenex are ready-to-use, individu- • Use for all syringe filter applications*
ally blister packaged units, offering high flow rates at low inlet pres- • Luer-lock outlet makes connection easy
sures, for rapid sterile filtration.
• Made of ultra-clean, high-purity plastics

Ordering Information Ordering Information

Sterile Syringe Filters All-Plastic Disposable Syringes
Pore Disc Membrane Part No. Description Capacity (mL)* Unit
Part No. Size (μm) Diameter (mm) Material Unit AS0-8408 Plastic Disposable Syringes, Luer-lock 3 100/pk
AF0-8455 0.2 28 CA Luer/Slip 50/pk AS0-8409 Plastic Disposable Syringes, Luer-lock 5 100/pk
AF0-8456 0.45 28 CA Luer/Slip 50/pk AS0-8410 Plastic Disposable Syringes, Luer-lock 10 100/pk
AF0-8457 0.2 28 PES Luer/Slip 50/pk AS0-8411 Plastic Disposable Syringes, Luer-lock 20 100/pk
AF0-8458 0.45 28 PES Luer/Slip 50/pk
AF0-8459 0.2 25 RC Luer/Slip 50/pk * Choose larger volume syringe to reduce force on syringe filter membrane. 10 mL syringe is recommended.

10 Phenomenex
Filtration Glassware
All-Glass Vacuum Mobile Designed for rapid filtration of particulate matter from HPLC sol-
vents, this unit is an excellent value. Protect your instrument and
Phase Filtration System column from costly damage by clarifying all your HPLC solvents
and buffer solutions before use. This vacuum filter assembly comes
FilterSys™ with a sample reservoir and receiving flask. A 47 mm diameter
membrane filter is placed between the fritted-glass support base
and the sample reservoir, secured in place by an aluminum clamp.
• Prevents pump and system component damage The support base itself is connected to the receiving flask by a
• Rapid filtration of buffers, organics and corrosive liquids vacuum-tight ground-glass joint. Only low-extractable borosilicate
glass and the membrane filter come into contact with the mobile
• Removes damaging microparticulates and bacterial
phase. The vacuum hose connection is made above the filtration
contaminants

MOBILE PHASE - FILTRATION

drip tip to prevent contamination from the vacuum line.
• HPLC and GC solvent and sample filtration
Recommended filter membranes: Nylon is a highly resistant mate-
rial and can be used with almost all laboratory solvents. Since Nylon
is hydrophilic, no prewetting of the filter is required. PTFE (Teflon® )
Solvent Pickup Valve
Adapter membrane filters are excellent for organic or other aggressive sol-
Graduated vent systems. Extremely low levels of extractables (plasticizers,
Funnel Compression contaminants, etc.) make this an excellent filter for trace analysis
Fitting Connector
(to Solvent Source) work. PTFE is hydrophobic, so it is not recommended for the filtra-
Phenex™
Clamp Filter Membranes
tion of aqueous solutions.

|
MOBILE PHASE FILTRATION SYSTEM
Ordering Information
Mobile Phase Filtration System
Part No. Description Unit
Complete Assembly
AH0-1566 FilterSys, 47 mm, 300 mL funnel with 1 L vacuum flask ea
to Vacuum Line
AH0-3314 FilterSys, 47 mm, 500 mL funnel with 2 L vacuum flask ea
AH0-3315 FilterSys, 47 mm, 1000 mL funnel with 4 L vacuum flask ea
Fritted Glass Component Parts
Support Base AH0-1567 Fritted support base, 47 mm, 40/35 taper ea
AH0-1568 Funnel, graduated, 300 mL, 47 mm ea
AH0-3323 Funnel, graduated, 500 mL, 47 mm ea
AH0-3324 Funnel, graduated, 1000 mL, 47 mm ea
AH0-1569 1 liter filter flask, 40/35 taper ea
AH0-3321 2 liter filter flask, 40/35 taper ea
Filter Flask
AH0-3322 4 liter filter flask, 40/35 taper ea
AH0-1570 Aluminum clamp, 47 mm ea
Filter Membranes
AF0-0503 Nylon, 0.2 µm, 47 mm 100/pk
AF0-0504 Nylon, 0.45 µm, 47 mm 100/pk
AF0-0514 PTFE, 0.5 µm, 47 mm 100/pk

WARNING: The apparatus should be

used with a water aspiration line, not a
true vacuum line, unless secured behind
an appropriate safety shield.

For compatible Solvent Pickup Adapter, see p. 12

For additional Filter Membranes, see p. 13

Verex™ Certified sample vials, inserts, caps, and seals are

guaranteed to ensure problem-free, reproducible performance
you can trust – all at competitive prices. See page 27

or Visit:
www.phenomenex.com/VialFinder

Phenomenex 11
 Filtration Glassware and Accessories
Solvent Reservoir and Reagent Increase Lab Safety with
Bottles HPLC / UHPLC Solvent
• Popular 1 and 2 liter sizes, equipped with 3-way valve cap Protection SecurityCAP™
• Low-leaching (low alkali), borosilicate glass
• Chemically inert, internal PTFE seal HPLC / UHPLC Solvent (Eluent)
These wide-mouth GL45 mobile phase reservoirs come in 1- and and Waste Protection
2-liter sizes. The reservoirs have easy-to-read volumetric mark-
ings to indicate the amount of solvent remaining. • Safer Laboratory Work Environment
Solvent vapors and gasses are restricted to the containers
The versatile 3-way valve cap supplied with each
FILTRATION - MOBILE PHASE

reservoir provides a totally-inert PTFE (Teflon) seal • Confidence During Quality and Safety Audits
against the solvents inside. Eliminate aluminum foil or parafilm covering solvent bottles
• Easy to Use
No more twisting tubes during bottle exchange
|

Ordering Information
Reservoir and Valve Cap Assembly*
MOBILE PHASE ACCESSORIES

Part No. Mfr. No. Description Unit

AH0-4142 3200 HPLC Reservoir, 1000 mL clear glass, GL45 ea
wide-mouth, includes 3-way Valve Cap
AH0-4143 3203 HPLC Reservoir, 2000 mL clear glass, GL45 ea
wide-mouth, includes 3-way Valve Cap
* Fittings not included. See p. 423 AQ0-2950
SecurityCAP Mobile Phase SecurityCAP Waste
Safety Caps and Filters Safety Caps and Filters

For Ordering and Additional SecurityCAP

Information, see pp. 417-418

Solvent Pickup Adapter

This glass adapter enables direct pickup of mobile phase solvent
for filtration using the Phenomenex FilterSys™ (see previous page).
Safe in-line filtration with the pickup adapter replaces the tedious
and dangerous pour-and-wait funnel filtration method. Eliminate
the possibilities of spilling solvents and breathing
toxic vapors. Replacing the funnel adapter on the
FilterSys unit, the 47 mm pickup adapter with flange
holds the membrane filter in place on top of the
fritted support base, which in turn is held by the
aluminum clamp (not included). The pickup adapter
draws solvent directly from the reagent bottle — the
safest way to transfer and filter solvents. The adapter
includes a 4  mm PTFE (Teflon) valve with ¼ in. OD
outlet, PTFE 90° elbow with compression fittings for
¼ in. OD tubing, and 3 feet of ¼ in. OD PTFE tubing.

Ordering Information
Solvent Pickup Adapter
Part No. Description Unit
AH0-2947 Mobile Phase Pickup Adapter, 47 mm ea

12 Phenomenex
Filtration Filter Membranes
Additional Filtration Products Filter Membranes
Phenex™
Regular sample filtration means:
• RC, Nylon, PTFE (Teflon® ), and other membranes available
• Less system downtime
• Wide selection of membrane sizes
• Fewer troubleshooting problems
Phenex PTFE (Teflon®) and Regenerated
• Improved results Cellulose (RC) membrane filters offer excellent
Removal of particulate matter to sub-micron levels is critical be- chemical resistance to almost all laboratory
fore any drug, tox, or dirty environmental sample is injected into an solvents and samples. They do not introduce
HPLC, GC or mass spectrometer. Also, products that remove ma- unwanted plasticizers or extractables into the

FILTRATION
trix components, interferents, and chemical garbage will improve sample or mobile phase. Since Regenerated
your results. Check out the following useful products: Cellulose (RC) is hydrophilic, filtering of aqueous
solvents is simple. No prewetting is required.
PTFE is hydrophobic and so is not recommended
for the filtration of aqueous solutions.

| FILTER MEMBRANES / ADDITIONAL PRODUCTS

Mini-Index Page No.

Filtration Products (General Laboratory) Ordering Information

Filter Membranes
• Syringe filters................................................................. 8 - 10
Pore Disc Membrane
• Membrane filters and filtration apparatus................ 11 - 13 Part No. Size (μm) Diameter (mm) Material Unit
Nylon
• Solid Phase Extraction (SPE) devices
AF0-0500 0.45 13 Nylon 100/pk
• Tubes and well plates.................................................. 50 - 78 AF0-0501 0.2 25 Nylon 100/pk
AF0-0502 0.45 25 Nylon 100/pk
• Vacuum manifolds ...................................................... 79 - 81
AF0-0503 0.2 47 Nylon 100/pk
Column Protection devices AF0-0504 0.45 47 Nylon 100/pk
PTFE
• SecurityGuard
AF0-0512 0.45 25 PTFE 100/pk
• UHPLC (2.1 to 4.6 mm ID)............................................................335 AF0-0514 0.45 47 PTFE 100/pk
• Analytical (2 to 8 mm ID)................................................... 330 - 334 Cellulose Acetate (CA)
• SemiPrep (9 to 16 mm ID)................................................. 332 - 334 AF0-8436 0.45 25 CA 100/pk
• PREP (18 to 29 mm ID) ..................................................... 332 - 334 AF0-8437 0.2 25 CA 100/pk
• PREP (30 to 49 mm ID) ..................................................... 332 - 334 AF0-8438 0.45 47 CA 100/pk
• SFC (18 to 29 mm ID) ....................................................... 332 - 334 AF0-8439 0.2 47 CA 100/pk
• SFC (30 to 49 mm ID) ....................................................... 332 - 334 Regenerated Cellulose (RC)
AF0-8440 0.45 13 RC 100/pk
• Inlet filters AF0-8441 0.2 13 RC 100/pk
• Biocompatible PEEK .....................................................................14 AF0-8442 0.2 25 RC 100/pk
AF0-8443 0.45 47 RC 100/pk
• Stainless steel ..............................................................................14
AF0-8444 0.2 47 RC 100/pk
• In-line filters Polyethersulfone (PES)
• Biocompatible PEEK AF0-8445 0.2 25 PES 100/pk
Analytical .................................................................................15 AF0-8446 0.45 25 PES 100/pk
SemiPrep .................................................................................16 AF0-8447 0.2 47 PES 100/pk
• Stainless steel AF0-8448 0.45 47 PES 100/pk
Cellulose Nitrate Ester (MCE)*
Analytical .................................................................................15
AF0-8454 0.45 47 MCE 100/pk
Prep .........................................................................................16

*MCE = Mixed Cellulose Esters

Above filter membranes are non-sterile.
Phenex is a trademark of Phenomenex.

For a useful Membrane Selection Guide, see p. 8

Phenomenex 13
 Filtration Mobile Phase Inlet Filters
Inlet Filters
Stainless Steel Metal-Free/Biocompatible
• Protects pumps and check valves • Biocompatible
• Easy to replace • Flat bottom design uses all available mobile phase
• Low cost • High surface area for long filter life
HPLC solvent inlet filters are used at the low pressure inlet side of The Solvent Saver™ Inlet Filter Unit utilizes a flat filter element that
the pump to help protect the check valves, injector and column sits parallel to the bottom of the HPLC reservoir. The design allows
from damaging particulate contamination. Solvent filters are con- the filter to draw all but the last 2 % of the mobile phase from the
structed of Hastelloy Steel and are available for 1/16 in. ID and 1/8 in. reservoir without drawing air into the system.
FILTRATION - MOBILE PHASE

ID tubing. Due to the large surface area of the cylindrical frit, virtu- The Solvent Saver Inlet Filter is manufactured by pressing a PEEK
ally no backpressure or cavitation is developed. The filter is easily filter element into an inert Teflon® housing. The top of the hous-
cleaned by backflushing or sonicating. ing has female 1/4 in.-28 threads to accept 1/8 or 1/16 in. OD tubing
via PEEK flangeless fittings and Tefzel® ferrule (sold separately) or
direct connect various size tubing using the Unifit adapter (sold
separately). The Unifit adapter slip-fits onto 1.5, 2.2 or 3.0 mm ID
tubing. This filter is excellent for sensitive biochromatography and
ion chromatography applications where metal surfaces may cor-
rode or interact with samples.
|
INLET FILTERS

Pump Inlet Filter

Unifit adapter 1/4 in.-28 flangeless
(sold separately AQ0-8339) fitting (included) attaches
slip-fits onto 1.5, to 1/8 in. OD tubing
2.2 or 3.0 mm ID
Ordering Information tubing

Solvent Inlet Filters - Stainless Steel

Part No. Description Unit
AF0-0356 Solvent Inlet Filter, 2 µm, for 1/16 in. ID tubing ea
AF0-0359 Solvent Inlet Filter, 2 µm, for 1/8 in. ID tubing ea
AT0-2955 Teflon Tubing, 5 ft. L x 1/8 in. OD x 1/16 in. (0.062 in.) ID ea
AT0-2956 Teflon Tubing, 10 ft. L x 1/8 in. OD x 1/16 in. (0.062 in). ID ea
AT0-8609 Teflon Tubing, 5 ft. L x 1/4 in. OD x 1/8 in. (0.125 in.) ID ea
AT0-8610 Teflon Tubing, 10 ft. L x 1/4 in. OD x 1/8 in. (0.125 in). ID ea

Important: Depending on the mobile phase, we recommend

Ordering Information
that you change your inlet filter every one to six months.
Solvent Saver Inlet Filter - Metal-Free
Part No. Description Unit
AH0-1562 Solvent Saver Inlet Filter with 10 µm PEEK ea
For disposable Syringe Filters offering convenient and
filter with Flangeless fitting for 1/8 in. OD tubing
economical sample and solvent filtration, see pp. 8 - 10
AQ0-8339 Solvent Saver Unifit Adapter, Tri-Step Tubing Connector, PEEK ea
AQ0-2949 Flangeless Nut and Ferrule for 1/16 in. OD tubing, 10/pk
¼ in.-28 threads, red Delrin
AQ0-2950 Flangeless Nut and Ferrule for 1/8 in. OD tubing, 10/pk
¼ in.-28 threads, green Delrin
AT0-2953 Teflon Tubing, 5 ft. L x 1/16 in. OD x 1/32 in. (0.031 in.) ID ea
AT0-2955 Teflon Tubing, 5 ft. L x 1/8 in. OD x 1/16 in. (0.062 in.) ID ea
AT0-2956 Teflon Tubing, 10 ft. L x 1/8 in. OD x 1/16 in. (0.062 in). ID ea
AT0-8610 Teflon Tubing, 10 ft. L x 1/4 in. OD x 1/8 in. (0.125 in). ID ea

14 Phenomenex
Filtration In-Line Filters
In-Line Filters
Stainless Steel (Analytical) KrudKatcher™
• Removes particulates from flow path • Universal fit to virtually all manufacturers’ analytical
• Minimizes sample peak dispersion columns

In-line Filters are available to protect ex- • Saves expensive columns and equipment from damaging
pensive HPLC columns from damaging microparticulates
microparticulates. Using one of these • Convenient, disposable in-line filter
filters between the injection valve and the There are two types of KrudKatchers depending on system back-
column is recommended for all HPLC systems. pressure and dead volume demands: the KrudKatcher Classic and

FILTRATION
The 3 mm diameter filter element is recommended for the KrudKatcher Ultra.
use with conventional 4.6 mm diameter columns. Column In-
line Filters are supplied with two 6 cm L x 0.007 in. ID connecting Whereas conventional in-line filters typically cost much more and
tubes. Pressure rating is 5000 psi (345 bar). often require replacement parts and tools, the KrudKatcher is a
simple, low-cost unit that is easily replaced and discarded when

| IN-LINE FILTERS
the backpressure indicates clogging or excessive particle build up.
Ordering Information The universal connection is compatible with all standard 1/16 in. 10-
Stainless Steel In-Line Filters (Analytical) 32 internal threaded end-fittings used in columns, guard columns,
Part No. Description Unit injector valves, and other LC equipment.
AF0-0377 In-line Filter with 0.5 µm Porosity x 3 mm dia. filter ea
AF0-0378 Replacement Filter Disks: 0.5 µm x 3 mm 5/pk

The KrudKatcher Classic:

• Pressure-rated to 5000 psi (345 bar)
Metal-Free/Biocompatible (Analytical) • Hand-tightened connection
• Biocompatible The KrudKatcher Classic houses an integrated 316 stainless steel
• Virtually no band broadening or peak distortion depth filter that efficiently removes microparticulates from the flow
• Easy fingertight connection stream with minimal contributions to system dead volume (2 μL).
The 2.0 µm filter has a PEEK filter body and the 0.5 µm has a stain-
This in-line filter assembly removes fine particles from the solvent less steel filter body.
stream without adding band broadening or peak distortion to your
separation. Microparticulates down to 0.5 μm are effectively re- Ordering Information
moved before they have a chance to plug your column and degrade KrudKatcher Classic Disposable In-Line Filter (Analytical)
your separation. The Polyglas™ frit with fluoropolymer frit assembly Part No. Description Unit
design is fully biocompatible and easily installed using fingertight AF0-5728 KrudKatcher Disposable Pre-Column Filter, 0.5 μm 10/pk
connections. Pressure-rated to 5000 psi (345 bar). AF0-5727 KrudKatcher Disposable Pre-Column Filter, 2.0 μm 10/pk

The KrudKatcher Ultra:

• Fits virtually all UHPLC / HPLC columns 1.0 to 4.6 mm ID
• Pressure rated to 20000 psi (1375 bar)
Ordering Information
Metal-Free/Biocompatible In-Line Filter (Analytical) • Extremely low dead volume minimizes sample peak dispersion
Part No. Description Unit The KrudKatcher Ultra filter body houses an integrated 2.0 µm
AF0-1736 In-line Filter, 0.5 µm Frit ea 316 stainless steel depth filter that efficiently removes micropar-
ticulates from the flow stream without contributing to system
backpressure or dead volume (<0.2 µL).

Ordering Information
For UHPLC system connections, see SecurityLINK™ KrudKatcher Ultra In-Line Filter (Analytical)
UHPLC fingertight fitting system on pp. 336-337 Part No. Description Unit
AF0-8497 HPLC KrudKatcher Ultra Column In-Line Filter, 3/pk
2.0 µm Depth Filter x 0.004 in. ID
For SecurityGuard™, KrudKatcher Ultra requires 5/16 in. wrench. Wrench not provided. See p. 417
the universal guard cartridge system, see p. 330

Phenomenex 15
 Filtration In-Line Filters and Column Couplers
In-Line Filters (cont’d)

Metal-Free/Biocompatible (SemiPrep) Analytical Column Couplers

• For columns 8 to 18 mm ID
Sure-Lok™ Coupler
• Effective pre-column filtration
• Universal and reusable
• Replaceable filter element
• Solvent resistant material
Phenomenex’s Biocompatible SemiPrep in-line filter holder with
• Low dead-volume connection
| FILTRATION

replaceable filter element (2 µm PEEK frit) will help protect your

column investment by safely removing particulate matter and in- • Compatible with all 10-32 internal-threaded fittings
soluble material from the mobile phase and sample matrix. The
filter consists of a stainless steel body, two PEEK end-fittings, and
a separate PEEK frit . When you need to replace the filter, simply Applications:
unscrew the assembly, remove the frit and replace it. This filter unit • Filter to column
IN-LINE FILTERS / COLUMN COUPLERS

can be placed in the flow path before or after the column with little
• Column to column
or no effect on peak shape. Pressure rated to 6000 psi (414 bar).
• Precolumn to column
Sure-Lok Coupler (PEEK)
• Column to detector
Ordering Information
Metal-Free/Biocompatible In-Line Filter (SemiPrep)
Part No. Description Unit Sure-Lok Couplers contain two Sure-Lok male nuts at either end of
AF0-8420 HPLC SemiPrep Column In-Line Filter ea a 5 cm long 1/16 in. tubing. The PEEK biocompatible coupler has all
2.0 µm Porosity x 10 mm dia. filter, Biocompatible parts composed of PEEK, including the 0.010 in. ID tubing. Finger-
AF0-8428 Replacement In-Line Filter Disk, PEEK, 5/pk tight to 5000 psi (345 bar).
2.0 µm Porosity x 10 mm dia.

Ordering Information
Sure-Lok Couplers (Analytical to SemiPrep)
Part No. Description Unit
AQ0-1392 PEEK Sure-Lok Coupler ea
Stainless Steel (PREP) AQ0-1393 PEEK Sure-Lok Coupler 10/pk
• Economical protection for preparative HPLC columns
and injectors
• For columns 19 to 30 mm ID
• Replaceable filter element
Preparative columns and the HPLC systems on which they are used PREP Column Coupler
are costly and must be protected against fouling. Phenomenex’s
PREP In-line Filter holder with replaceable filter element (2 µm stain-
less steel frit) will help protect your investment by safely removing
particulate matter and insoluble material from the mobile phase and
sample matrix. The filter unit can be placed in the flow path before
or after the column with little or no
effect on peak shape. This versatile
filter can also protect check valves, Ordering Information
injectors and detectors. Pressure PREP Column Coupler
Part No. Description Unit
rated to 8000 psi (551 bar).
AQ0-8376 PREP Coupler, Stainless Steel Tube, Nuts, and Ferrules ea
10-32 Threads, 1/16 in. OD x 0.030 in. ID
Ordering Information
Stainless Steel In-Line Filters (PREP)
Part No. Description Unit
AF0-7866 HPLC PREP Column In-line Filter, S.S., ea
2.0 μm Porosity x 21.2 mm dia.
Protect your column and equipment
AF0-7867 Replacement In-Line Filter Disks, S.S., 5/pk
2.0 µm Porosity x 21.2 mm dia. with Phenex™ Syringe Filters
AQ0-7877 PREP Replacement O-Rings, 1 in. OD x 2/pk Filtering your sample helps prevent column and frit blockage, undue
7/8 in. ID x 1/16 in. CS, Fluorocarbon wear on detectors, pumps, valves, injector seals, and abnormally
high operating pressures. Non-filtered samples can also lead to
AT0-0465 Capillary S.S. Tubing, 0.020 in. ID x 0.062 in. 5/pk
(1/16 in.) OD x 10 cm L non-reproducible results and significant instrument downtime.
AT0-0466 Capillary S.S. Tubing, 0.020 in. ID x 0.062 in. 5/pk See page 8 or Visit:
(1/16 in.) OD x 20 cm L www.phenomenex.com/SFfinder

For SecurityGuard™,
the universal guard cartridge system, see p. 330

16 Phenomenex
 Syringes
Sample Handling

17 - 26

Syringes
“ ”
Technical assistance is always
very thorough.

Sherri Tapp
Symbiotic Research LLC
The opinions stated herein are solely those of the speaker
and not necessarily those of any company or organization.
GC Syringes.............................................................................. 18
HPLC Syringes......................................................................... 23
General Use Syringes............................................................... 25
 GC Syringes
Technical Information
Needle Gauge Dimensions
Gauge OD ID
26 0.018 in. 0.46 mm 0.0102 in. 0.26 mm
26s 0.019 in. 0.47 mm 0.0050 in. 0.13 mm
25 0.021 in. 0.51 mm 0.0102 in. 0.26 mm
23s 0.025 in. 0.64 mm 0.0060 in. 0.15 mm
23 0.025 in. 0.64 mm 0.0132 in. 0.34 mm
22s 0.028 in. 0.72 mm 0.0060 in. 0.15 mm
22 0.028 in. 0.72 mm 0.0162 in. 0.41 mm

Needle Point Styles

Style Description Advantage
AS/Cone Exclusively for Agilent autosamplers

2, BV (Beveled tip) Optimal septum piercing for reduced septum coring

SYRINGES - SAMPLE HANDLING

3, LD (Blunt tip) General sample pipetting and HPLC injectors

5 (Conical with side hole) Side hole for sample filling and dispensing, headspace

H (Dome with side hole) Side hole for sample filling and dispensing. Dome tip minimizes septum coring.

Needle / Needle Connection Styles

Style Description Manufacturer Advantage
ASN Autosampler Needle Hamilton® For use with autosamplers
ASRN Autosampler Removable Needle Hamilton For use with autosamplers
|

RN Removable Needle Hamilton Allows freedom to change needle style or replace broken needles
GC SYRINGES

N Cemented Needle Hamilton Low dead volume, for low-volume syringes

KH Knurled Hub Hamilton Able to handle pressure up to 6000 psig
LTN Luer Tip Cemented Needle Hamilton Fixed needle with Luer Tip, for mid-volume syringes
TLL PTFE (Teflon®) Luer Lock Hamilton Luer Lock with locking hub for use with syringe filters
F Fixed Needle SGE® Economical and more reproducible
R Removable Needle SGE Allows freedom to change needle style or replace broken needles
FLL Fixed Luer Lock SGE Allows use with syringe filters
LL Luer Lock SGE Allows use with syringe filters

GC Autosampler Syringes
for Agilent® GC 7673, 7683, 7693, and 6850 Autosampler Systems
Ordering Information
Hamilton Syringes
Volume Needle/Needle Needle Needle Similar to
(μL) Connection Gauge Length Style Model Mfr. No Mfr. No.* Unit Part No.
Microliter Syringes
5 ASN 23s 1.71 in. Agilent 75 87987 9301-0892 20168 ea AS0-4386
5 ASN 23s 1.71 in. Agilent 75 87990 20170 6/pk AS0-7641
5 ASN 26s 1.71 in. Agilent 75 87989 21230 6/pk AS0-8683
5 ASN 23s-26s 1.71 in. Agilent 75 87993 24593 ea AS0-8380
10 ASRN 23s 1.71 in. Agilent 701 80357 24795 ea AS0-8836
10 ASN 23s 1.71 in. Agilent 701 80387 9301-0713 20167 ea AS0-4387
10 ASN 23s 1.71 in. Agilent 701 80390 9301-0725 20169 6/pk AS0-4388
10 ASN 26s 1.71 in. Agilent 701 80389 24599 6/pk AS0-4389
10 ASN 23s-26s 1.71 in. Agilent 701 80393 24596 ea AS0-8684
10 ASN 23s-26s 1.71 in. Agilent 701 80391 24600 6/pk AS0-8685
Teflon Tip Gastight® Syringes
10 ASN 23s-26s 1.71 in. Agilent 1701 80079 n/a ea AS0-8837
10 ASN 23s 1.71 in. Agilent 1701 80080 n/a ea AS0-9079

*Similar to but not always an exact equivalent to the original manufacturer’s product.

18 Phenomenex
GC Syringes
GC Autosampler Syringes (cont’d)
for Agilent® GC 7673, 7683, 7693, and 6850 Autosampler Systems (cont’d)
SGE® Syringes
Volume Needle/Needle Needle Needle Similar to
(μL) Connection Gauge Length Style Model Mfr. No Mfr. No.* Unit Part No.
Microliter Syringes
5 F 23 42 mm Cone SK-5F-HP-0.63 001814 24783 6/pk AS0-8687
5 F 26 42 mm Cone SK-5F-HP-0.47 001804 24782 6/pk AS0-8688
5 F 23s-26s 42 mm Cone SK-5F-HP-0.63/0.47 001822 21214 6/pk AS0-8689
5 F 23-26 42 mm Cone 5F-HP-0.63*0.47 001821 5181-1273 21210 ea AS0-5208
10 F 26 42 mm Cone SK-10F-HP-47 002804 24786 6/pk AS0-7637
10 R 23 42 mm Cone 10RHP-0.63 002815 24795 ea AS0-8690
10 F 23 42 mm Cone 10F-HP-0.63 002810 24785 ea AS0-8691
10 F 26 42 mm Cone SK-10F-HP-0.47 002804 24786 6/pk AS0-7637
10 F 23 42 mm Cone SK-10F-HP-0.63 002814 24787 6/pk AS0-8692
AS0-8693

SYRINGES - SAMPLE HANDLING

10 F 23s-26s 42 mm Cone 10F-HP-0.63/0.47 002821 21212 ea
10 F 23s-26s 42 mm Cone SK-10F-HP-0.63/0.47 002822 21215 6/pk AS0-8694
Teflon® Tip Gastight® Syringes
10 F 23 42 mm Cone HF-HP-GT-0.63 002812 24789 ea AS0-8695
10 F 23-26 42 mm Cone 10F-HP-GT-0.63/0.47 002826 5181-1267 ea AS0-5209
10 F 23-26 42 mm Cone SK10F-HP-FT-0.63/0.47 002827 5181-3361 6/pk AS0-5210

for PerkinElmer® AutoSystem™ and Clarus® 500 GC Systems

Ordering Information

|
SGE Syringes

GC SYRINGES
Volume Needle/Needle Needle Needle Similar to
(μL) Connection Gauge Length Style Model Mfr. No Mfr. No.* Unit Part No.
Microliter Syringes
0.5 R 23 70 mm Cone 0.5BR-PE-0.63 000478 24811 ea AS0-8697
5 F 23 70 mm Cone 5F-PE-0.63 001954 24813 ea AS0-8698

for Varian® 8000 Series GC Systems

Ordering Information
SGE Syringes
Volume Needle/Needle Needle Needle Similar to
(μL) Connection Gauge Length Style Model Mfr. No Mfr. No.* Unit Part No.
Microliter Syringes
10 R 25 53 mm Side Hole 10R-VA8X-II 002924 24852 ea AS0-8699
10 F 26 50 mm Cone 10F-C/F-5/0.47C 002980 24922 ea AS0-8700
10 F 26 50 mm Bevel 10F-VA8400-5/0.47 002950 21202 ea AS0-8701

for Shimadzu® AOC 9GC Systems

Ordering Information
Hamilton Syringes
Volume Needle/Needle Needle Needle Similar to
(μL) Connection Gauge Length Style Model Mfr. No Mfr. No.* Unit Part No.
Microliter Syringes
5 RN 26s 2 in. /51 mm 2 75RN 87930 24617 ea AS0-8702
5 N 26s 2 in. /51 mm 2 75N 87900 24938 ea AS0-4390
10 RN 26s 2 in. /51 mm 2 701RN 80330 24530 ea AS0-0100

Ordering Information
SGE Syringes
Volume Needle/Needle Needle Needle Similar to
(μL) Connection Gauge Length Style Model Mfr. No Mfr. No.* Unit Part No.
Microliter Syringes
10 R 23 42 mm Cone 10R-S-0.63 002898 24845 ea AS0-8703

*Similar to but not always an exact equivalent to the original manufacturer's product. GC Autosampler Syringes continued on next page.

Phenomenex 19
 GC Syringes
GC Autosampler Syringes (cont’d)
for Thermo Scientific® GC Systems
Ordering Information
SGE® Syringes
Volume Needle/Needle Needle Needle Similar to
(μL) Connection Gauge Length Style Model Mfr. No Mfr. No.* Unit Part No.
Microliter Syringes
10 F 26 80 mm Cone 10F-C/F-8/0.47C 002992 24924 ea AS0-8704
10 R 26 80 mm Cone 10R-C/F-8/0-0.47C 002993 24934 ea AS0-8705

for CTC/LEAP GC Systems

Ordering Information
Hamilton® Syringes
Volume Needle/Needle Needle Needle Similar to
SYRINGES - SAMPLE HANDLING

(μL) Connection Gauge Length Style Model Mfr. No Mfr. No.* Unit Part No.
Microliter Syringes
1.2 N 26 2 in./51 mm Cone 7701.2N 203185 22755 ea AS0-8706

Ordering Information
SGE Syringes
Volume Needle/Needle Needle Needle Similar to
(μL) Connection Gauge Length Style Model Mfr. No Mfr. No.* Unit Part No.
Microliter Syringes
10 F 23 50 mm Cone 10F-C/F-5/0.63 002981 24923 ea AS0-7638
10 R 23 50 mm Cone 10R-C/F-5/0.63 002984 24932 ea AS0-8709
|

10 F 26 50 mm Cone 10F-C/F-5/0.47C 002980 24922 ea AS0-8700

GC SYRINGES

10 F 26 50 mm Cone SK10F-C/F-5/0.47C 002986 24925 6/pk AS0-8711

10 R 26 50 mm Cone 10R-C/F-5/0.47C 002982 24930 ea AS0-8712

*Similar to but not always an

exact equivalent to the original
GC Manual Syringes manufacturer's product.

Ordering Information
Hamilton Syringes
Volume Needle/Needle Needle Needle Similar to
(μL) Connection Gauge Length Style Model Mfr. No Mfr. No.* Unit Part No.
Microvolume Syringes
5 N 26s 2 in./51 mm 2 75N 87900 24938 ea AS0-4390
5 RN 26s 2 in./51 mm 2 75RN 87930 24617 ea AS0-8702
10 N 26s 2 in./51 mm 2 701N 80300 20174 ea AS0-0077
10 N 26s 2 in./51 mm 2 701N 80366 20175 6/pk AS0-0016
10 RN 26s 2 in./51 mm 2 701RN 80330 24530 ea AS0-0100
25 N 22s 2 in./51 mm 2 702N 80400 24531 ea AS0-0078
10 N 26s 2 in./51 mm 5 701NPT5 80339 24967 ea AS0-0094
Positive Displacement Syringes
0.5 KH 25 2.75 in./70 mm 2 7000.5 86259 24545 ea AS0-8715
1.0 KH 22 2.75 in./70 mm 2 7101 86211 24549 ea AS0-8716
1.0 KH 25s 2.75 in./70 mm 2 7001 80135 24547 ea AS0-1918
1.0 KH 25s 2.75 in./70 mm 3 7001 80100 24548 ea AS0-8717
2.0 KH 25 2.75 in./70 mm 2 7002 88411 24551 ea AS0-8718
5.0 KH 24 2.75 in./70 mm 2 7105 88011 24555 ea AS0-8719
Teflon Tip Gastight Syringes
10 N 26s 2 in./51 mm 2 1701 80000 24557 ea AS0-4391
10 RN 26s 2 in./51 mm 2 1701 80030 24558 ea AS0-1898
25 N 22s 2 in./51 mm 2 1702 80200 24559 ea AS0-8720
25 RN 22s 2 in./51 mm 2 1702 80230 24560 ea AS0-1899
50 N 22s 2 in./51 mm 2 1705 80900 24561 ea AS0-0061
50 RN 22s 2 in./51 mm 2 1705 80930 24562 ea AS0-1900
100 N 22s 2 in./51 mm 2 1710 81000 24563 ea AS0-0062
100 RN 22s 2 in./51 mm 2 1710 81030 24564 ea AS0-1901
250 N 22s 2 in./51 mm 2 1725 81100 24567 ea AS0-0063

20 Phenomenex
GC Syringes
GC Manual Syringes (cont’d)
Ordering Information
Hamilton® Syringes (continued)
Volume Needle/Needle Needle Needle Similar to
(μL) Connection Gauge Length Style Model Mfr. No Mfr. No.* Unit Part No.
Teflon Tip Gastight® Syringes (continued)
®

250 RN 22s 2 in./51 mm 2 1725 81130 24568 ea AS0-1902

500 LTN 22 2 in./51 mm 2 1750 81217 24571 ea AS0-0064
500 RN 22 2 in./51 mm 2 1750 81230 24572 ea AS0-1903
1 mL LTN 22 2 in./51 mm 2 1001 81317 24575 ea AS0-0065
1 mL RN 22 2 in./51 mm 2 1001 81330 24576 ea AS0-8721
1 mL TLL – – w/o slots 1001 81320 24578 ea AS0-1907
2.5 mL TLL – – w/o slots 1002 81420 24584 ea AS0-1908
2.5 mL RN 22 2 in./51 mm 2 1002 81430 24582 ea AS0-8722
2.5 mL N 22 2 in./51 mm 2 1002 81417 24581 ea AS0-8723

SYRINGES - SAMPLE HANDLING

10 mL TLL – – w/o slots 1010 81620 20179 ea AS0-1910

Ordering Information
SGE® Syringes
Volume Needle/Needle Needle Needle Similar to
(μL) Connection Gauge Length Style Model Mfr. No Mfr. No.* Unit Part No.
Microvolume Syringes
5 F 26 50 mm 2 5F 001000 24700 ea AS0-8725
5 R 26 50 mm 2 5R 001050 24701 ea AS0-8726

|
10 F 26 50 mm 2 10F 002000 24702 ea AS0-8727

GC SYRINGES
10 F 26 50 mm 2 SK-10F 002030 24715 6/pk AS0-8728
10 R 26 50 mm 2 10R 002050 24703 ea AS0-8729
Positive Displacement Syringes
1.0 R 23 70 mm 2 1BR-7BV 000506 24775 ea AS0-8738
1.0 R 26 70 mm Cone 1BR-7/0.47 000570 24776 ea AS0-8739
Teflon Tip Gastight Syringes
10 F 26 50 mm 2 10F-GT 002200 24725 ea AS0-8741
25 F 25 50 mm 2 25F-GT 003200 24727 ea AS0-8743
50 F 25 50 mm 2 50F-GT 004200 24729 ea AS0-8744
50 R 25 50 mm 2 50R-GT 004250 24730 ea AS0-8745
100 F 25 50 mm 2 100F-GT 005200 24734 ea AS0-8746
100 R 25 50 mm 2 100R-GT 005250 24735 ea AS0-8747
250 F 25 50 mm 2 250F-GT 006200 24738 ea AS0-8748
250 R 25 50 mm 2 250R-GT 006250 24739 ea AS0-8749
500 F 25 50 mm 2 500F-GT 007200 24742 ea AS0-8750
1 mL RN 23 50 mm Bevel 1MDR-GT 008100 24750 ea AS0-8845
1 mL LL – – – 1MDF-LL-GT 008025 24752 ea AS0-0120
2.5 mL R 23 50 mm Bevel MDR-GT 008500 – ea AS0-8752
5 mL LL – – – 5MDR-LL-GT 008760 24757 ea AS0-0121
10 mL LL – – – 10MDR-LL-GT 008960 24759 ea AS0-0122

*Similar to but not always an

exact equivalent to the original
manufacturer's product.

Phenomenex 21
 GC Syringes
VICI® Series A-2 Gas Syringes Filtration Products from Phenomenex
• Push-button valve and smaller volumes “Filtration is the easiest way to improve your results, guard your
• Pressure-Lok® design offers gas-tight injections system components from damage, and protect your column
investment”.
• Inject without carrier backflushing to sharpen peaks
and improve separations
• Leak-tight to 250 psi - liquids and gases Phenex™
• No plunger "blow out" at elevated pressures Syringe Filters
• Increase column lifetime and save money!
The A-2 features a push-button valve, which allows sample storage • Ensure more accurate, consistent results
up to 250 psi in syringes as small as 25 µL. This is useful with small • Eliminate damaging microparticlates
liquid samples containing low-boiling components that would be
lost through evaporation using ordinary syringes.
The positive rear stop (in 100 µL and larger sizes) prevents plunger Particulates can damage expensive equipment, valves, columns and
SYRINGES - SAMPLE HANDLING

blowout at elevated pressures, protecting against sample loss or pumps. They can also lead to erratic analytical results. Pre-filtering
operator injury. Like the Series A gas syringe, the A-2 has all the samples prior to analysis is critical in preventing column and frit
standard Pressure-Lok features such as a PTFE plunger tip, PTFE- blockage, undue wear on valve seals, and abnormally high operating
sealed needle and ultra-smooth bore. Replacement components pressures.
are available for easy repair. Sample or Mobile Phase Filter Membrane
Volume (mL) (diameter, mm) Format
≤2 4 Syringe filter
2 to 10 15 Syringe filter
10 to 100 25-28 Syringe filter
> 100 47 Membrane disk
> 1000 90 Membrane disk
|
GC SYRINGES

Ordering Information1
Part No. Pore Size (µm) Phenex Membrane
4 mm Diameter (500/pk)
AF0-3103-52 0.45 RC
Ordering Information AF0-3102-52 0.45 PTFE6
VICI Precision Sampling Syringes AF3-3107-52 0.45 NY
Part No. Mfr. No. Description Capacity (µL) Unit AF0-3203-52 0.20 RC
AF0-3202-52 0.20 PTFE6
AS0-4739 050023 Series A-2 Syringe 25 ea
AF3-3207-52 0.20 NY
AS0-4740 050024 Series A-2 Syringe 50 ea
AS0-4741 050025 Series A-2 Syringe 100 ea 15 mm Diameter (500/pk)
AF0-2103-52 0.45 RC
AS0-4742 050031 Series A-2 Syringe 250 ea
AF0-2102-52 0.45 PTFE6
AS0-4743 050032 Series A-2 Syringe 500 ea
AF0-2107-52 0.45 NY
AS0-4744 050033 Series A-2 Syringe 1000 (1 mL) ea
AF0-2203-52 0.20 RC
AS0-4745 050034 Series A-2 Syringe 2000 (2 mL) ea
AF0-2202-52 0.20 PTFE6
AS0-4746 050035 Series A-2 Syringe 5000 (5 mL) ea
AF0-2207-52 0.20 NY
17 mm Diameter (500/pk)
Notes: (1) All other VICI Precision Sampling products not listed are available.
(2) Removable needle: 0.028 in. x 0.005 in. x 2.00 in., bevel, open end on 25, 50 and 100 µL AF6-5106-52 0.45 PVDF
(p/n 943050, 3/pk); 0.029 in. x 0.012 in. x 2.00 in., bevel, open end on all others (p/n 943051, 3/pk). 25–28 mm Diameter (500/pk)
AF0-8103-52 5 0.45 RC
AF0-8108-52 7 0.45 PES3
AF0-1102-52 0.45 PTFE6
AF0-1107-52 0.45 NY
AF0-8B09-52 7 0.45 GF/CA2,3,4
AF0-8203-52 5 0.20 RC
AF0-8208-52 7 0.20 PES3
AF0-1202-52 0.20 PTFE6
AF0-1207-52 0.20 NY
AF0-8A09-52 7 0.20 GF/CA2,3,4,7
AF0-8515-52 7 1.20 GF2,3
30 mm Diameter (500/pk)
AF6-6106-52 0.45 PVDF
Housing is made of medical-grade polypropylene (PP), unless otherwise indicated.
Above syringe filters are non-sterile.
1. Additional membrane types available. 3. Housing material is methacrylate 5. 26 mm diameter.
2. Glass fiber filters are 28 mm diameter butadiene styrene (MBS) polymerisate. 6. Hydrophobic membrane. Can be made
and made of borosilicate. They will Also known as Cyrolite®. hydrophilic by pre-wetting with IPA.
remove 90 % of all particles >1.2 µm. 4. Cellulose acetate is surfactant-free. 7. 28 mm diameter.

For additional Phenex Syringe Filters and a useful Membrane

Syringes continued on next page. Selection Guide, see pp. 8-10

22 Phenomenex
HPLC Syringes
for Rheodyne®, Altex and Valco® (VISF-2) Injectors
Ordering Information
Hamilton Syringe
Volume Needle/Needle Needle Needle Similar to
(μL) Connection Gauge Length Style Model Mfr. No Mfr. No.* Unit Part No.
5 RN 22s 2 in./51 mm 3 65 87943 ea AS0-3353
10 N 22s 2 in./51 mm 3 701 80365 21250 ea AS0-0022
25 N 22s 2 in./51 mm 3 702 80465 21251 ea AS0-0023
50 N 22s 2 in./51 mm 3 705 80565 21252 ea AS0-0024
100 N 22s 2 in./51 mm 3 710 80665 21253 ea AS0-0025
250 N 22 2 in./51 mm 3 725 80765 21254 ea AS0-0026
500 N 22 2 in./51 mm 3 750 80865 ea AS0-0027

for Waters® U6K Injector, Removable Needle

Ordering Information
Hamilton Syringe

SYRINGES - SAMPLE HANDLING

Volume Needle/Needle Needle Needle Similar to
(μL) Connection Gauge Length Style Model Mfr. No Mfr. No.* Unit Part No.
10 RN 25s 1.97 in./50 mm 3 801 84815 21255 ea AS0-0028
25 RN 25s 1.97 in./50 mm 3 802 84816 21256 ea AS0-0029
50 RN 25s 1.97 in./50 mm 3 805 84817 21257 ea AS0-0030
100 RN 25s 1.97 in./50 mm 3 810 84818 21258 ea AS0-0031

Hamilton® 10 mL Gastight® Priming Syringe, for Waters HPLC Pumps

(Models 6000, 6000A, 501, 510, 610 and 610E)
Ordering Information
Hamilton Syringe

|
Volume Needle/Needle Needle Needle Similar to
(mL) Connection Gauge Length Style Model Mfr. No Mfr. No.* Unit Part No.

HPLC SYRINGES
10 W – – – 1010 81610 21265 ea AS0-1906

Replacement Needles
Point Style #2 (Beveled Tip)
Ordering Information
Hamilton Replacement Needles
Volume Needle/Needle Needle Needle Similar to
(μL) Connection Gauge Length Style Model Mfr. No Mfr. No.* Unit Part No.
2.5-100 RN 26 2 in./51 mm 2 RN NDL 7758-04 6/pk AS0-1904
25-100 RN 26 2 in./51 mm 2 RN NDL 7758-02 24939 6/pk AS0-4392
25-100 RN 22s 2 in./51 mm 2 RN NDL 7758-03 24940 6/pk AS0-4393
0.250-10 mL RN 22s 2 in./51 mm 2 RN NDL 7779-03 24944 6/pk AS0-4398

Point Style #3 (Blunt Tip)

Ordering Information
Hamilton Replacement Needles
Volume Needle/Needle Needle Needle Similar to
(μL) Connection Gauge Length Style Model Mfr. No Mfr. No.* Unit Part No.
2.5-100 RN 22s 2 in./51 mm 3 RN NDL 7770-01 24941 6/pk AS0-4394
0.250-10 mL RN 22 2 in./51 mm 3 RN NDL 7780-04 24945 6/pk AS0-4397
0.250-10 mL RN 22s 2 in./51 mm 3 RN NDL 7780-03 6/pk AS0-4400

Needle point #2 (22 ° bevel) is used for GC and

most applications that require the puncturing Needle point style #3 (90 °) is appropriate for
of a septum. HPLC applications.
Removable Needle (RN, R)

*Similar to but not always an exact equivalent to the original manufacturer's product.

Cemented Needle (N, F)

For Rheodyne HPLC Sample Injectors, see pp. 428-431 HPLC Syringes continued on next page.

Phenomenex 23
 HPLC Syringes
Replacement Needles (cont’d)
for Rheodyne®, Valco® HPLC Injectors, 2 in. Fixed Needles
Ordering Information
SGE® Syringe
Volume Needle/Needle Needle Needle Similar to
(μL) Connection Gauge Length Style Model Mfr. No Mfr. No.* Unit Part No.
10 F 22 2 in./51 mm LD 10F-LC 002301 24860 ea AS0-0142
25 F 22 2 in./51 mm LD 25F-LC 003300 24861 ea AS0-0143
50 F 22 2 in./51 mm LD 50F-LC 004300 24862 ea AS0-0144
100 F 22 2 in./51 mm LD 100F-LC 005300 24863 ea AS0-0145
250 F 22 2 in./51 mm LD 250F-LC 006300 24864 ea AS0-0146
500 F 22 2 in./51 mm LD 500F-LC 007300 24865 ea AS0-0147

for Rheodyne, Valco HPLC Injectors, 2 in. Removable Needles

SYRINGES - SAMPLE HANDLING

Ordering Information
SGE Syringe
Volume Needle/Needle Needle Needle Similar to
(μL) Connection Gauge Length Style Model Mfr. No Mfr. No.* Unit Part No.
10 R 22 2 in./51 mm LD 10R-GT-LC-SS 002313 24866 ea AS0-4370
25 R 22 2 in./51 mm LD 25R-GT-LC-SS 003312 24867 ea AS0-4371
100 R 22 2 in./51 mm LD 100R-GT-LC-SS 005312 24869 ea AS0-4373
500 R 22 2 in./51 mm LD 500R-GT-LC-SS 007312 24871 ea AS0-4375

for PerkinElmer®, Fixed Needles

|

Ordering Information
HPLC SYRINGES

SGE Syringe
Volume Needle/Needle Needle Needle
(μL) Connection Gauge Length Style Model Mfr. No Unit Part No.
5 F 23 2.76 in./70 mm Cone - 001957 ea AS0-7636

for CTC/LEAP, Fixed Needles

Ordering Information
SGE Syringe
Volume Needle/Needle Needle Needle
(μL) Connection Gauge Length Style Model Mfr. No Unit Part No.
10 F 23 1.97 in./50 mm Cone - 002981 ea AS0-7638

Removable Needle (RN, R)

Cemented Needle (N, F)

*Similar to but not always an

exact equivalent to the original
manufacturer's product.

For Rheodyne HPLC Sample Injectors,

see pp. 428-431

24 Phenomenex
General Use Syringes
General Use Syringes for:
Gastight syringes have a polymer tipped
• Pipetting plunger, often PTFE, which essentially
wipes the interior of the syringe barrels.
• Diluting This reduces the risk of deposition
on the barrel which may cause cross-
• Delivering contamination or plunger seizing
Plunger Tip

Hamilton® 1700 Series Gastight® Syringes, Needle Point Style 2 (Beveled)

Ordering Information

SYRINGES - SAMPLE HANDLING

Hamilton Syringe
Volume Needle/Needle Needle Needle Similar to
(μL) Connection Gauge Length Style Model Mfr. No Mfr. No.* Unit Part No.
10 RN 26s 2 in./51 mm 2 1701 80030 24558 ea AS0-1898
25 RN 22s 2 in./51 mm 2 1702 80230 24560 ea AS0-1899
50 RN 22s 2 in./51 mm 2 1705 80930 24562 ea AS0-1900
100 RN 22s 2 in./51 mm 2 1710 81030 24564 ea AS0-1901
250 RN 22s 2 in./51 mm 2 1725 81130 ea AS0-1902
500 RN 22s 2 in./51 mm 2 1750 81230 ea AS0-1903

|
GENERAL USE SYRINGES
Hamilton 1700 Series Gastight Syringes, Needle Point Style 3 (Blunt)
Ordering Information
Hamilton Syringe
Volume Needle/Needle Needle Needle Similar to
(μL) Connection Gauge Length Style Model Mfr. No Mfr. No.* Unit Part No.
10 RN 22s 2 in./51 mm 3 1701 80065 ea AS0-4380
25 RN 22s 2 in./51 mm 3 1702 80265 24560 ea AS0-4381
50 RN 22s 2 in./51 mm 3 1705 80965 24562 ea AS0-4382
100 RN 22s 2 in./51 mm 3 1710 81065 24564 ea AS0-4383
250 RN 22s 2 in./51 mm 3 1725 81165 24568 ea AS0-4384
500 RN 22s 2 in./51 mm 3 1750 81265 24572 ea AS0-4385

Hamilton 1000 Series Gastight Syringes

Ordering Information
Hamilton Syringe
Volume Needle/Needle Needle Needle Similar to
(mL) Connection Gauge Length Style Model Mfr. No Mfr. No.* Unit Part No.
1 TLL – – w/o slots 1001 81320 24578 ea AS0-1907
2.5 TLL – – w/o slots 1002 81420 24584 ea AS0-1908
5 TLL – – w/o slots 1005 81520 20178 ea AS0-1909
10 TLL – – w/o slots 1010 81620 20179 ea AS0-1910
– TLL 22 2 in./51 mm 2 KF722 90122 6/pk AS0-1915
– TLL 16 2 in./51 mm 2 KF716 90116 6/pk AS0-1916

Needle point #2 (22 ° bevel) is used for GC and

most applications that require the puncturing Needle point style #3 (90 °) is appropriate for
of a septum. HPLC applications.

*Similar to but not always an exact equivalent to the original manufacturer's product. General Use Syringes continued on next page.

Phenomenex 25
 General Use Syringes
SGE® Syringes, Gas Tight Luer Lock
Ordering Information
SGE Syringe
Volume Needle/Needle Needle Needle
(mL) Connection Gauge Length Style Model Mfr. No Unit Part No.
1 F - - - 1 mL GT 008025 ea AS0-0120
5 R - - - 5 mL GT 008760 ea AS0-0121
10 R - - - 10 mL GT 008960 ea AS0-0122

SGE Replacement Needles, Removable, for Gas Tight Luer Lock Syringes
Ordering Information
SGE Replacement Needles
Volume Needle/Needle Needle Needle Similar to
(μL) Connection Gauge Length Style Model Mfr. No Mfr. No.* Unit Part No.
- R 23 1.97 in./50 mm BV NLL-5/23 039802 24763 5/pk AS0-2016
- R 23 1.97 in./50 mm H NLL-5/23H 039803 2/pk AS0-2017
SYRINGES - SAMPLE HANDLING

*Similar to but not always an exact equivalent to the original

manufacturer's product.

Phenex™ Syringe Filters

• Increase column lifetime and save money!
• Ensure more accurate, consistent results
• Eliminate damaging microparticlates

Ordering Information1
|

Part No. Pore Size (µm) Phenex Membrane

GENERAL USE SYRINGES

4 mm Diameter (500/pk)

AF0-3103-52 0.45 RC
AF0-3102-52 0.45 PTFE6
All-Plastic Disposable Syringes AF3-3107-52
AF0-3203-52
0.45
0.20
NY
RC
• Use for all syringe filter applications AF0-3202-52 0.20 PTFE6
AF3-3207-52 0.20 NY
• Luer-lock outlet makes connection easy
15 mm Diameter (500/pk)
• Capacities ranging from 3 to 20 mL AF0-2103-52 0.45 RC
AF0-2102-52 0.45 PTFE6
• Made of ultra-clean, high-purity plastics AF0-2107-52 0.45 NY
AF0-2203-52 0.20 RC
Ordering Information AF0-2202-52 0.20 PTFE6
All-Plastic Disposable Syringes AF0-2207-52 0.20 NY
Part No. Description Capacity (mL)* Unit 17 mm Diameter (500/pk)
AS0-8408 Plastic Disposable Syringes, Luer-lock 3 100/pk AF6-5106-52 0.45 PVDF
AS0-8409 Plastic Disposable Syringes, Luer-lock 5 100/pk 25–28 mm Diameter (500/pk)
AS0-8410 Plastic Disposable Syringes, Luer-lock 10 100/pk AF0-8103-52 5 0.45 RC
AS0-8411 Plastic Disposable Syringes, Luer-lock 20 100/pk AF0-8108-52 7 0.45 PES3
*Choose larger volume syringe to reduce force on syringe filter membrane. 10 mL syringe is recommended. AF0-1102-52 0.45 PTFE6
AF0-1107-52 0.45 NY
AF0-8B09-52 7 0.45 GF/CA2,3,4
AF0-8203-52 5 0.20 RC
AF0-8208-52 7 0.20 PES3
Recommended Plastic Syringes AF0-1202-52 0.20 PTFE6
for use with Phenex Syringe Filters AF0-1207-52 0.20 NY
Part No. AS0-8410: AF0-8A09-52 7 0.20 GF/CA2,3,4,7
10 mL Plastic Disposable Syringes, Luer-Lock 100/pk AF0-8515-52 7 1.20 GF2,3
Part No. AS0-8411: 30 mm Diameter (500/pk)
20 mL Plastic Disposable Syringes, Luer-Lock 100/pk AF6-6106-52 0.45 PVDF

Housing is made of medical-grade polypropylene (PP), unless otherwise indicated.

Above syringe filters are non-sterile.
1. Additional membrane types available. 3. Housing material is methacrylate 5. 26 mm diameter.
Pressure Forces Generated from Plastic Syringes: 2. Glass fiber filters are 28 mm diameter butadiene styrene (MBS) polymerisate. 6. Hydrophobic membrane. Can be made
and made of borosilicate. They will Also known as Cyrolite®. hydrophilic by pre-wetting with IPA.
20 mL can generate 2.0 bar remove 90 % of all particles >1.2 µm. 4. Cellulose acetate is surfactant-free. 7. 28 mm diameter.
10 mL can generate 3.4 bar
5 mL can generate 5.2 bar
3 mL can generate 6.9 bar For additional Phenex Syringe Filters and a useful Membrane
Selection Guide, see pp. 8-10

26 Phenomenex
 Vials
Sample Handling

27 - 48

Sample Vials
Verex Filter Vials..........................................................28-29

“
Autosampler Vials, Caps, Septa, and Inserts
Verex Vials Quality and Certification...............................30-31
8 mm...................................................................................... 39 I am very pleased to do
9 mm...........................................................................32, 36-38 business with Phenomenex. . . . for me

”
10 mm.............................................................................. 32, 40 you are a kind of benchmark for other
11 mm...............................................................................32-35 companies.
13 mm.................................................................................... 41
Limited Volume Specialty, 12 x 32 mm................................. 32 Jos Mecklenfeld
QPS, Netherlands
Shell Vials............................................................................. 41
The opinions stated herein are solely those of the speaker
Headspace Vials................................................................ 42 and not necessarily those of any company or organization.

VOA / ASE Vial Kits.......................................................... 43

Storage Vial Kits................................................................ 43
Verex-EU Vials and Caps..........................................44-46
Verex-IN Vials and Caps................................................. 47
Verex Vial Resources....................................................... 48
 Verex Filter Vial Products Certified Filter Vials

Filter Vial Products

Verex Filter Vials are designed to offer quick and efficient sample Verex Filter Vials Offer:
preparation by incorporating a syringe, filtration membrane, vial, • Simplified workflow
and cap/septa all into one product. Each filter vial is comprised of
two parts: the external vial to be filled with sample and an internal • Less system downtime
plunger equipped with a filtration membrane and cap that includes • Reduced lab waste
a pre-slit PTFE/Silicone septa. Simply, dispense your sample into
• Broad chemical compatibility
the external vial and compress the internal plunger to filter. This
produces a particulate-free sample ready for analysis. Verex Filter
Vials are a standard 12 x 32 mm vial and can be easily loaded into
an autosampler. Filter Vial Selection Guide

1.
4 Products 1 Product Choose a pore size based on chromatographic
media and flow path
For HPLC/UHPLC columns packed with ≤ 3 μm chromatographic media, a Verex Filter Vial
with 0.20 µm filter membrane is recommended. Filter membranes with a 0.20 µm pore size
provides an effective barrier against unwanted particulates entering the system flow path.

vs.
This reduces column plugging, leading to longer column lifetimes and increased system up-
time. For HPLC columns with > 3 μm chromatographic media, a Verex Filter Vial with 0.45 µm
filter membrane is recommended.
FILTER VIAL PRODUCTS | VIALS - SAMPLE HANDLING

Particle Size Flow Path Effective Filter

(μm) (μm) Pore Size (μm)
5 0.72 0.45
3 0.43 0.20
2.6 0.38 0.20
Traditional Sample Filtration Filter Vial
1.7 0.25 0.20

2.6 µm

Flow Path

Reduce Sample Loss and

Contamination
Eliminate multiple transfers with 2.
this all-in-one filtration device
Choose a filter membrane according to the
characteristics of your sample and filtering objective
Membrane Type Recommended Uses
RC Hydrophilic Regenerated Cellulose filter membranes exhibit
(Regenerated Cellulose) fast-flow and ultra-low protein and non-specific binding
Internal Plunger
characteristics and are compatible with a broad range of
aqueous and mixed-organic solutions. This membrane is
broadly recommended as an excellent general purpose/
high-performance samples’ filter for most applications.
PTFE Polytetrafluoroethylene is an inherently hydrophobic
External Vial (Polytetrafluorethylene) membrane excellent for filtration of organic-based, highly
acidic or basic samples and solvents. Widely used in chro-
matography, it is especially well suited for the clarification
of non-aqueous and organic samples.

Particulate-Free Sample NY Nylon has inherent hydrophilic characteristics and works

(Nylon) well for filtration and clarification of many aqueous and
The filter membrane is attached
mixed-organic solvent solutions.
to the internal plunger to ensure
removal of particulates PES Polyethersulfone membranes exhibit very fast-flow and
(Polyethersulfone) ultra-low protein binding characteristics and ensures
removal of particulate matter. This membrane is ideally
suited for use in many biological studies and is broadly
recommended for filtering critical biological samples, tissue
culture media, additives and buffers.
Save Your Sample
With a low dead volume of 30 µL, PVDF Hydrophilic Polyvinylidene Fluoride filter membranes provide
(Polyvinylidene Fluoride) high flow rates and throughput, low extractables, and broad
more of your sample can be chemical compatibility. This membrane binds less protein
analyzed than nylon or PTFE membranes and is typically used in
clarification of biological samples or dissolution testing.

28 Phenomenex
 Verex ™
Filter Vial Products

Multivitamin Tablet Analysis by HPLC-UV

mAU
LC-UV Conditions
2 Unfiltered Multivitamin Tablet
Column: Synergi™ 4 μm Hydro-RP
400 No change in peaks with
complex tablet formulations! Filtered Multivitamin Tablet Dimension: 150 x 4.6 mm
350 Part No.: 00F-4375-E0
Pressure (bar): 142
300 Mobile Phase: A: 20 mM Potassium phosphate +
0.1 % Hexane sulfonate, pH 3.0
250 5 B: Acetonitrile
Gradient: Time (min) %B
200
4 0 3
3 3
150 18 50
18.1 3

App ID 26459
100 1 20 3
Flow Rate: 1.5 mL/min
50 3 Temperature: 30 ˚C
Detection: UV @ 210 nm
0
Injection Volume: 3 μL
0 2 4 6 8 10 12 14 min Instrument: Agilent® 1100 HPLC with Quaternary
Pump
Sample Preparation
Sample: 1. Thiamine
Make: 1 mg/mL concentrations of each sample with water from the tablet matrix. 2. Pyridoxine
For Riboflavin, add 1-2 drops of Ammonium Hydroxide to help dissolve 3. Pantothenic Acid
Mix: 50 µL of each sample into a mastermix

SAMPLE HANDLING - VIALS | FILTER VIAL PRODUCTS

4. P-Aminobenzoic Acid
Load: 250 µL of mastermix into Verex Filter Vial 0.45 µm, NY (AR0-F207-12) 5. Riboflavin
Inject: 3 µL final eluate onto HPLC-UV

Monoclonal Antibody (mAbs) Fragments

AU
2 F(ab')2 LC-UV Conditions
Unfiltered Sample
Column: Synergi™ 4 μm Hydro-RP
0.048 Minimize sample loss and
Filtered Sample Dimension: 150 x 4.6 mm
keep consistent method
Part No.: 00F-4375-E0
performance
Pressure (bar): 142
0.036 Mobile Phase: A: 0.1 % TFA in Water
B: 0.1 % TFA in Acetonitrile
Gradient: Time (min) %B
1 scFc 0 25
0.024 10 45
11 80
13 80
App ID 26412

13.1 25
0.012 17.1 25
Flow Rate: 0.8 mL/min
Detector: UV-Vis @ 280 nm
0.000
Sample: Nivolumab, IdeZ Digested

3.60 3.96 4.32 4.68 5.04 5.40 5.76 6.12 6.48 6.84 min

Sample Preparation
Reconstitute: FabRICATOR® Z in 50 µL ddH2O to a concentration of 40 units/µL
Add: 1 unit FabRICATOR Z / 1 µg IgG. Final Concentration should be 0.5-10 mg/mL
Digest: Incubate mixture for 16 hrs at 37 °C
Load: Sample into Verex Filter Vial 0.45 µm, PES (AR0-F208-12)
Inject: 2 µL final eluate onto HPLC-UV

• Diverse color options make identification and reorder Ordering Information

simple and easy! Description Membrane Pore Part No. Unit
Verex Filter Vial-RC 0.20 µm AR0-F103-12 100/pk
(Regenerated Cellulose) 0.45 µm AR0-F203-12 100/pk
Verex Filter Vial-PTFE 0.20 µm AR0-F102-12 100/pk
(Polytetrafluoroethylene) 0.45 µm AR0-F202-12 100/pk
Verex Filter Vial-NY 0.20 µm AR0-F107-12 100/pk
(Nylon) 0.45 µm AR0-F207-12 100/pk
Verex Filter Vial-PES 0.20 µm AR0-F108-12 100/pk
(Polyethersulfone) 0.45 µm AR0-F208-12 100/pk
Verex Filter Vial-PVDF 0.20 µm AR0-F106-12 100/pk
(Polyvinylidene Fluoride) 0.45 µm AR0-F206-12 100/pk

Phenomenex 29
 Verex Certified Vial Products Certified Vial Products

Vials for UHPLC / HPLC / GC / SFC and MS Analysis

Leading in Quality, Delivery, and Support Vials and inserts are made of inert, high-purity, chemically-stable
borosilicate glass to exacting specifications, individually heat-
The most critical point of your analysis is when you put your sample treated to burn off any impurities, then packaged in a cleanroom
in the vial. Verex certified sample vials, inserts, caps and seals are environment. Choose from crimp, snap, and screw types or mix and
guaranteed to ensure problem-free, reproducible performance with match vials and caps to your application. Most vials are offered with
consistent results you can trust. or without a write-on marking spot or “patch”. For easy selection
or purchase convenience, our assembled vial kits are ready to go!

All Vials, Caps, and Kits Verex caps / seals / closures are made to exacting specifications,
giving a tight seal each and every time. The aluminum crimp seals,
• Advanced manufacturing plastic screw and snap caps come with highly pure septa mate-
• Multi-step QA/QC rial and are available in pre-slit and non-slit formats. Economical
press-fit and specially formulated bonded-in septa styles are both
• Cleanroom packaged
available. And our mass spec certified (MSQ) screw caps provide
• Certified you with a state-of-the art solution for your most challenging, sensi-
With the demand for better product reproducibility and performance tive applications.
in glass, septa and caps, we developed Verex HPLC / GC vial A variety of complete vial kits are available to meet the most de-
products with high quality materials to exceed industry specifica- manding needs of chromatographers, from routine analytical to
tions and tolerances. Verex innovative precision products provide high-sensitivity work:
AUTOSAMPLER VIALS | VIALS - SAMPLE HANDLING

air-tight, leak-free seals to safely transfer and store your most

important samples. Through extensive testing, we’ve created
guaranteed-fit vial products that offer compatibility with virtually • 33-Expansion clear glass (USP Type 1 borosilicate,
any autosampler for trouble-free operation. Class A), 51A amber glass
With tightly controlled 100 % traceable manufacturing processes, • Limited volume (high sample recovery) vials
we re-defined the standards for lot traceability and certification
• Silanized (deactivated) glass
of every Verex product to ensure a uniform product with optimum
performance for any application. For those with the most sensitive • Polypropylene for biocompatibility
applications, choose Verex-Certified PLUS or Certified MSQ prod- • Certified and special MS-certified (caps and vials)
ucts for excellent ultra-grade cleanliness. Bottom line, Verex is your
sample transfer and storage solution.

1 2 3
Three Levels Certified Certified PLUS
(Cert+)
Certified MSQ
(Cert + MSQ)
(Mass Spec Quality)
Ultra Clean
Specially designed and treated for mass spectrometry (MS) applications. ✓
Low Bleed
Special cap / closure processing, treating, and testing for low bleed of residual
organics. For high sensitivity GC-MS and LC-MS applications.
✓ ✓
Dimensionally Verified
Machine vision cameras and gauges inspect critical dimensions and tolerances
to produce uniform, defect-free products.
✓ ✓ ✓
Lot Traceable
Vial and cap packs are 100 % traceable through the manufacturing process.
Certificate of Conformance available by lot number upon request.
✓ ✓ ✓
Clean Room Packaged
Ensures particulate- and contaminate-free products for clean chromatography. ✓ ✓ ✓
Part Number Extension Certificate of Conformance Included; Certificate of Conformance Included;
No Extension
Extension -C Extension -M

Part Number Example AR0-8952-13 AR0-8952-13-C AR0-8952-13-M

Need help matching your current

vials and caps to Verex? Visit:
www.phenomenex.com/VialFinder

30 Phenomenex
Verex ™
Certified Vial Products

Vials for UHPLC / HPLC / GC / SFC and

MS Analysis (cont’d)
All Verex products are fully lot-tested and certified. The de-
tails of product specifications and testing are available at
www.phenomenex.com/verex. The “Certified PLUS” rating signi-
fies the highest quality available from Phenomenex. A Certificate
of Conformance is included. Depending on the product, Certified
PLUS may indicate, for example, special processing or treating
and/or testing for low bleed or residual organics. Certified products
offer excellent performance, especially for high sensitivity, mass
spectrometry applications.

From the manufacturing of our products to their

RIGHT CHOICE
timely delivery and superior customer support,
we are dedicated to continually improving our
processes to consistently meet or exceed your
expectations.

SAMPLE HANDLING - VIALS | AUTOSAMPLER VIALS

Certified Plus
MS Quality

Vials, The Most Critical Part of Your Analysis!

Though they may look alike, not all vials offer equivalent perfor- irreproducible results. Begin every analysis with high quality Verex
mance. Variations in product quality can adversely affect your products to minimize troubleshooting delays, and costly, unneces-
chromatography, leading to mystery peaks, loss of analytes, and sary rework.

100 Soak Blank Verex Products:

Scan ES+
• Improved analytical results 5.98e9
• Fewer contaminants
%

• Smoother baselines

100
Verex Certified MSQ Kit Scan ES+
3 5.98e9
%

Certified MSQ
0

100 Company A Competing Products Risk:

• Mystery / ghost peaks Scan ES+
LC-MS Certified Kit • Increased interferences
5.98e9
%

• Inaccurate results

100 Company B Scan ES+

LC-MS Certified Kit 5.98e9
%

100 Company C
Scan ES+
LC-MS Certified Pre-Cleaned Kit 5.98e9
%

0 m/z
100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000

Comparative separations may not be representative of all applications.

Phenomenex 31
 Snap to Vials, Specialty

nserts for 9-425

Inserts Screw-Tops
for 9-425 VialsVials
Screw-Tops
Verex
Snap to Vials, Specialty ™
Certified Vial Products

Inserts for 9-425 Screw-Tops Vials

12 x 32 mm Limited Volume Specialty Vials and Kits

12 x 32mm Specialty Vials

Type and Description Finish Material Total Volume Residual Volume Available as Page Number Part Numbers

High-Recovery Convenience 37 AR0-9981-13

CD Vial Kits 37 AR0-9982-13
9 mm Screw Thread Glass 1.5 mL < 20 µL
Snap to Vials,
Snap Specialty
to Vials, Specialty
(certified 36 AR0-9985-13-C
Center-draining and regular) 36 AR0-9986-13-C
12 x 32mm Specialty Vials

Max‑Recovery Vials
34 AR0-3680-12
CD Vial 11 mm Snap or (regular)
Snap to Vials, Specialty Glass 1.5 mL < 2 µL 36 AR0-9987-13-C
9 mm Screw Thread Convenience Kits
36 AR0-9988-13-C
Center-draining (certified)

Insert Vial
Vials 34 AR0-3630-13
11 mm Snap Glass 500 µL < 2 µL
(regular) 34 AR0-3631-13
µVial i2V

12 x12
32mm Specialty VialsVials
AUTOSAMPLER VIALS | VIALS - SAMPLE HANDLING

x 32mm Specialty

34 AR0-9671-13
34 AR0-9672-13
Insert Vial Convenience Kits
11 mm Snap or 37 AR0-9973-13
Glass 300 µL < 4 µL (certified and
9 mm Screw Thread 37 AR0-9974-13
µVial i3 (Qsert) regular)
36 AR0-9974-13-C
12 x 32mm Specialty Vials

34 AR0-3625-13
Insert Vial 11 mm Crimp or 33 AR0-3725-13
Vials
11 mm Snap or Glass 475 µL < 4 µL 33 AR0-3726-13
(regular)
Inserts for 9-425 Screw-Tops Vials µVial i3 (Qsert) 9 mm Screw Thread 37 AR0-3920-13
37 AR0-3921-13

Insert Vial
Vials 40 AR0-3020-13
10 mm Screw Thread Glass 450 µL < 2 µL
(regular) 40 AR0-3021-13
µVial i3 (Qsert)

1.7 mL Vials AR0-39P0-13

Plastic Vial 9 mm Screw Thread Polypropylene 700 µL < 2 µL (certified and 37 AR0-39P1-13
Snap to Vials, Specialty 300 µL regular) AR0-39P2-13

40 AR0-3040-13
11 mm Crimp or 34 AR0-3640-13
Micro Vial with Tapered Base 11 mm Snap or 34 AR0-3641-12
Vials
9 mm Screw Thread Glass 1.5 mL < 4 µL 33 AR0-3740-13
(regular)
v-Vial or 10 mm Screw 33 AR0-3741-13
Thread 37 AR0-3940-13
37 AR0-3941-13

12 x 32mm Specialty Vials

9-425 neck finish represents a vial with a diameter of 9 mm

across the outside of the threads and a thread style of 425.

32 Phenomenex
Verex ™
Certified Vial Products

12 x 32 mm, 11 mm Crimp-Top Vial Products

Crimp-Top Vials, 2.0 mL
• Cleaner vials eliminate ghost peaks and contaminants
• Used with most autosamplers, including Agilent®, Thermo
Scientific®, Waters®
• Larger-opening “wide-mouth” style prevents broken
needles and system downtime
• Precision neck improves crimping

Ordering Information
Description 1000/pk
Standard Opening
Vial, Crimp, 2 mL Clear, No Patch AR0-3700-13
Vial, Crimp, 2 mL Clear, w/ Patch AR0-3710-13
Vial, Crimp, 2 mL Amber, w/ Patch AR0-3711-13
Wide Mouth Opening
Vial, Crimp, 2 mL Wide Mouth, Clear, No Patch AR0-37K0-13

SAMPLE HANDLING - VIALS | AUTOSAMPLER VIALS

Vial, Crimp, 2 mL Wide Mouth, Clear, w/ Patch AR0-37L0-13
Vial, Crimp, 2 mL Wide Mouth, Amber, No Patch AR0-37K1-13
Vial, Crimp, 2 mL Wide Mouth, Amber, w/ Patch AR0-37L1-13
Download FREE
Verex vials and caps poster guide at:
www.phenomenex.com/verex

Limited Volume Specialty Crimp-Top Vials

• Microsampling (center-draining and fused-insert vials)
Ordering Information
Description 100/pk 1000/pk
Vial, Crimp, v-Vial Clear, No Patch* AR0-3740-12 AR0-3740-13
Vial, Crimp, v-Vial Amber, No Patch* — AR0-3741-13
Vial, Crimp, µVial i3 (Qsert), Clear, w/ Patch AR0-3725-12 AR0-3725-13
Vial, Crimp, µVial i3 (Qsert), Amber, w/ Patch AR0-3726-12 AR0-3726-13
*51-Expansion glass.

Seals / Closures for Crimp-Top Vials

• Excellent for volatile samples
• Extra clean to eliminate contamination
• Colored aluminum
Ordering Information
Description 1000/pk
Seal, 11 mm Diameter, Crimp, PTFE/Silicone, silver AR0-5780-13
Seal, 11 mm Diameter, Crimp, PTFE/Silicone/PTFE, silver AR0-5760-13 Save Money. Bulk discounts available.
Seal, 11 mm Diameter, Crimp, PTFE/Rubber, silver AR0-5740-13 Timely delivery. Excellent support.
Seal, 11 mm Diameter, Crimp, PTFE/Rubber, blue AR0-5742-13
Seal, 11 mm Diameter, Crimp, PTFE/Rubber, red AR0-5741-13
Seal, 11 mm Diameter, Crimp, PTFE/Rubber, green AR0-5743-13 Request a Quote. Want to make sure
Seal, 11 mm Diameter, Crimp, PTFE/Rubber, gold AR0-574G-13 you’re getting the best pricing on vials? Visit:
Seal, 11 mm Diameter, Crimp, PTFE, silver AR0-5710-13 www.phenomenex.com/Verex

For Vial Inserts for Standard Opening Crimp-Top Vials, use 5 mm Diameter Inserts, see p. 39
For Vial Inserts for Wide Mouth Crimp-Top Vials, use 6 mm Diameter Inserts, see p. 38

Phenomenex 33
 Verex ™
Certified Vial Products

12 x 32 mm, 11 mm Snap-Top Vials and Kits

Convenience Kits – Snap-Top Vials and Seals / Closures
• Precision formed for autosampler fit
• Larger-opening “wide-mouth” style prevents broken needles and system downtime
• Rigorous quality testing for consistent performance
• Precise vial depth provides consistent sample recoveries
Ordering Information†
Description 1000/pk
Vial Kit, Snap Cap, 2 mL Clear, w/ Patch + PTFE/Silicone AR0-9721-13
Vial Kit, Snap Cap, 2 mL Clear + PTFE/Silicone, preSlit AR0-9727-13
No write-on patch unless otherwise indicated
†

Convenience Kits – Limited Volume Specialty Snap-Top Vials and Seals / Closures
• Microsampling (fused-insert vials)
• Cleaner vials eliminate ghost peaks and contaminants
AUTOSAMPLER VIALS | VIALS - SAMPLE HANDLING

Ordering Information
Description 100/pk 1000/pk
Vial Kit, Snap, µVial i3 (Qsert), Clear w/ Patch + PTFE/Silicone AR0-9671-12 AR0-9671-13
Vial Kit, Snap, µVial i3 (Qsert), Clear w/ Patch + PTFE/Silicone, preSlit AR0-9672-12 AR0-9672-13

Snap-Top Vials*, 2.0 mL

• Great for less volatile samples or high-throughput labs
• Save time, without crimping or twisting
Ordering Information
Description 1000/pk
Vial, Snap, 2 mL Clear, No Patch AR0-3600-13
Vial, Snap, 2 mL Clear, w/ Patch AR0-3610-13
Vial, Snap, 2 mL Amber, w/ Patch AR0-3611-13
Vial, Snap, 2 mL Clear, w/ Patch, Silanized AR0-3613-13
Vial, Snap, 2 mL Amber, w/ Patch, Silanized AR0-3614-13
* Vials can accept either snap or crimp-tops to effectively seal the vial.

Limited Volume Specialty Snap-Top Vials

• For small volume microsamples (center-draining and fused-insert vials)
• One piece vial with fused-insert
Ordering Information
Description 100/pk 1000/pk
Vial, Snap, v-Vial i2V Clear, No Patch AR0-3640-12 AR0-3640-13
Vial, Snap, v-Vial i2V Amber, No Patch AR0-3641-12 —
Vial, Snap, µVial i2V Clear, No Patch AR0-3630-12 AR0-3630-13
Vial, Snap, µVial i2V Amber, No Patch AR0-3631-12 AR0-3631-13
Vial, Snap, µVial i3 (Qsert), Clear, w/ Patch AR0-3625-12 AR0-3625-13
Vial, Snap, Maximum Recovery (CD), Clear, No Patch AR0-3680-12 AR0-3680-13

51-Expansion glass.

Simply choose the vial closure convenience kit for your application and you’ll also receive a free dispenser box!

For Vial Inserts for Snap-Top Vials, use 6 mm Diameter Inserts, see p. 38

34 Phenomenex
Verex ™
Certified Vial Products

12 x 32 mm, 11 mm Snap-Top Vials and Kits (cont’d)

Seals / Closures for Snap-Top Vials
• Specially designed for secure fit
• Easily snap cap onto vial and pull cap off
Ordering Information
Description 1000/pk
No-Slit
Seal, 11 mm Diameter, Snap, PTFE/Silicone, blue AR0-5652-13
Seal, 11 mm Diameter, Snap, PTFE/Silicone, Cert+, blue AR0-5652-13-C
Seal, 11 mm Diameter, Snap, PTFE/Silicone, natural AR0-5656-13
Seal, 11 mm Diameter, Snap, PTFE/Silicone, red AR0-5651-13
Seal, 11 mm Diameter, Snap, PTFE/Silicone, green AR0-5653-13
Seal, 11 mm Diameter, Snap, PTFE/Silicone, yellow AR0-5654-13
Seal, 11 mm Diameter, Snap, PTFE/Rubber, natural AR0-5646-13
Seal, 11 mm Diameter, Snap, PTFE, blue AR0-5612-13
Seal, 11 mm Diameter, Snap, PTFE/Silicone/PTFE, blue AR0-5661-13
preSlit
Seal, 11 mm Diameter, Snap, PTFE/Silicone preSlit, blue AR0-5672-13
Seal, 11 mm Diameter, Snap, PTFE/Silicone preSlit, natural AR0-5676-13

SAMPLE HANDLING - VIALS | AUTOSAMPLER VIALS

Seal, 11 mm Diameter, Snap, PTFE/Silicone preSlit, red AR0-5671-13
Seal, 11 mm Diameter, Snap, PTFE/Silicone preSlit, green AR0-5673-13
Seal, 11 mm Diameter, Snap, PTFE/Silicone preSlit, yellow AR0-5674-13
Seal, 11 mm Diameter, Snap, LDPE preSlit, natural* AR0-5690-13

*Cap made of 100 % LDPE with no PTFE or Silicone

Make the Switch from

your Current Vials
and Caps to Verex!
2 Easy Steps:
Enter your current vial or
cap part number 1
1
Order the recommended
Verex part numnber 2

2 Finding the Verex replacement to

your current vials and caps is EASY.
Use our online web tool to find the
guaranteed Verex product match.

Visit:
www.phenomenex.com/VialFinder

Phenomenex 35
 Verex ™
Certified Vial Products

12 x 32 mm, 9 mm (9-425) Screw-Top Vials and Kits

Convenience Kits – Certified PLUS (Cert+) Mass Spec Quality (MSQ)
9 mm Screw-Top Vials and Caps with Locked-Fit Septa
• For your most demanding analysis; high sensitive detectors
• HPLC and LC-MS tested and certified RIGHT CHOICE

• State-of-the-art design and manufacture

3
Ordering Information
Description 1000/pk
Cert+ MSQ Vial Kit, 9 mm, 2 mL Clear w/ Patch + MSQ PTFE/Silicone AR0-992A-13-M Certified Plus
MS Quality
Cert+ MSQ Vial Kit, 9 mm, 2 mL Amber w/ Patch + MSQ PTFE/Silicone AR0-992B-13-M

Convenience Kits – Certified PLUS (Cert+) 9 mm Screw-Top Vials

and Caps with Bonded-In Septa
• Certified for cleanliness and enhanced quality
• Polyethylene-Starburst cap is an excellent choice for inertness
2
Ordering Information
Description 1000/pk
AUTOSAMPLER VIALS | VIALS - SAMPLE HANDLING

Cert+ Vial Kit, 9 mm, 2 mL Clear w/ Patch + PTFE/Silicone cap AR0-9921-13-C
Cert+ Vial Kit, 9 mm, 2 mL Clear w/ Patch + PE-Starburst cap AR0-9927-13-C
Cert+ Vial Kit, 9 mm, 2 mL Amber w/ Patch + PTFE/Silicone, preSlit cap AR0-9926-13-C

Convenience Kits – Certified PLUS (Cert+) Limited Volume Specialty

9 mm Screw-Top Vials and Caps
• Kits for microsampling (center-draining and fused-insert vials)
• Lower trace contaminants for higher sensitivity work

2
Ordering Information*
Description 100/pk 1000/pk
Cert+ Vial Kit, 9 mm, Maximum Recovery (CD) Clear + PTFE/Silicone cap AR0-9987-12-C AR0-9987-13-C
Cert+ Vial Kit, 9 mm, Maximum Recovery (CD) Clear + PTFE/Silicone, preSlit cap AR0-9988-12-C AR0-9988-13-C
Cert+ Vial Kit, 9 mm, High Recovery (CD) Clear + PE-Starburst cap AR0-9985-12-C AR0-9985-13-C
Cert+ Vial Kit, 9 mm, High Recovery (CD) Amber + PE-Starburst cap AR0-9986-12-C AR0-9986-13-C
Cert+ Vial Kit, 9 mm, µVial i3(Qsert) Clear w/ Patch + PTFE/Silicone, preSlit cap AR0-9974-12-C AR0-9974-13-C
*No write-on patch unless otherwise indicated

-B = Bonded-In Septa.  Cap is one piece, constructed of ultra-pure, medical-grade polyethylene.

Convenience Kits – 9 mm Screw-Top Vials and Caps with Bonded-In Septa
• Sample volume range from 0.2 to 1.5 mL
Ordering Information
best seller
Description 1000/pk
Vial Kit, 9 mm, 2 mL Clear + PTFE/Silicone AR0-9901-13
Vial Kit, 9 mm, 2 mL Clear + PTFE/Silicone, preSlit AR0-9903-13
Vial Kit, 9 mm, 2 mL Clear w/ Patch + PTFE/Silicone AR0-9921-13
Vial Kit, 9 mm, 2 mL Clear w/ Patch + PTFE/Silicone, Silanized AR0-9921-13-D
Vial Kit, 9 mm, 2 mL Clear w/ Patch + PTFE/Silicone, preSlit AR0-9925-13
Vial Kit, 9 mm, 2 mL Clear w/ Patch + PTFE/Silicone, preSlit, Silanized AR0-9925-13-D
Vial Kit, 9 mm, 2 mL Clear + PTFE/Silicone/PTFE AR0-9905-13
Vial Kit, 9 mm, 2 mL Clear + PTFE/Rubber AR0-9907-13
Vial Kit, 9 mm, 2 mL Clear w/ Patch + PTFE/Rubber, preSlit AR0-9914-13
Vial Kit, 9 mm, 2 mL Amber w/ Patch + PTFE/Silicone AR0-9922-13
Vial Kit, 9 mm, 2 mL Amber w/ Patch + PTFE/Silicone, Silanized AR0-9922-13-D
Vial Kit, 9 mm, 2 mL Amber w/ Patch + PTFE/Silicone, preSlit AR0-9926-13 Learn More. For additional product selection and
detailed information visit:
Vial Kit, 9 mm, 2 mL Amber w/ Patch + PTFE/Silicone, preSlit, Silanized AR0-9926-13-D
Vial Kit, 9 mm, 2 mL Amber w/ Patch + PTFE/Silicone/PTFE AR0-9923-13 www.phenomenex.com/Verex
Vial Kit, 9 mm, 2 mL Amber w/ Patch + PTFE/Rubber AR0-9912-13

36 Phenomenex
Verex ™
Certified Vial Products

12 x 32 mm, 9 mm (9-425) Screw-Top Vials and Kits (cont’d)

Convenience Kits – Limited Volume Specialty 9 mm Screw-Top
Vials and Caps with Bonded-In Septa
• Kits for microsampling (center-draining and fused-insert vials)
• CD and Qsert vials offer superior performance over vials with loose inserts
Ordering Information*
Description 100/pk 1000/pk
Vial Kit, 9 mm, High Recovery (CD) Clear + PTFE/Silicone AR0-9981-12 AR0-9981-13
Vial Kit, 9 mm, High Recovery (CD) Clear + PTFE/Silicone, preSlit AR0-9982-12 AR0-9982-13
Vial Kit, 9 mm, µVial i3 (Qsert) Clear w/ Patch + PTFE/Silicone AR0-9973-12 AR0-9973-13
Vial Kit, 9 mm, µVial i3 (Qsert) Clear w/ Patch + PTFE/Silicone, preSlit AR0-9974-12 AR0-9974-13
Vial Kit, 9 mm, PP, 300 µL + PTFE/Silicone** AR0-9991-12 AR0-9991-13
Vial Kit, 9 mm, PP, 300 µL + PTFE/Silicone, preSlit** AR0-9992-12 AR0-9992-13
*No write-on patch unless otherwise indicated. **Vial made of Polypropylene.

Convenience Kits – Assembled 9 mm Screw-Top

Vials and Caps with Bonded-In Septa

SAMPLE HANDLING - VIALS | AUTOSAMPLER VIALS

• Cap and septa attached to vials
• Ready to use
• Save time and labor
Ordering Information*
Description 1000/pk
Vial Kit, 9 mm, 2 mL Clear w/ Patch + PTFE/Silicone, preSlit AR0-9925-13-A
Vial Kit, 9 mm, 2 mL Amber w/ Patch + PTFE/Silicone, preSlit AR0-9926-13-A
*-A = Assembled

9 mm Screw-Top Vials, 2.0 mL

Need help matching your current
• Used with most autosamplers, including Agilent®, Thermo Scientific®, vials and caps to Verex? Visit:
Waters® and many others www.phenomenex.com/VialFinder
• Performs as well as crimp or snap vials
• Offers improved cap convenience and accessibility (easy on, easy off)
Ordering Information
Description 1000/pk
Vial, 9 mm Screw, 2 mL Clear, No Patch AR0-3900-13
Vial, 9 mm Screw, 2 mL Amber, No Patch AR0-3901-13
Vial, 9 mm Screw, 2 mL Clear, w/ Patch AR0-3910-13
Vial, 9 mm Screw, 2 mL Amber, w/ Patch AR0-3911-13
Vial, 9 mm Screw, 2 mL Clear, w/ Patch, Silanized AR0-3960-13

Limited Volume Specialty 9 mm Screw-Top Vials

• Microsampling Qsert and v-Vial center-draining vials
• Qsert fused-insert vials ensure proper seating of the cap
• Extremely low residual volume
• Polypropylene (PP) vial kits for bio- or ion chromatography, or pH-sensitive samples
Ordering Information
Description 100/pk 1000/pk
Vial, 9 mm Screw, µVial i3 (Qsert) Clear, No Patch AR0-3920-12 AR0-3920-13
Vial, 9 mm Screw, µVial i3 (Qsert) Amber, No Patch AR0-3921-12 AR0-3921-13
Vial, 9 mm Screw, v-Vial Clear, No Patch* AR0-3940-12 AR0-3940-13
Vial, 9 mm Screw, v-Vial Amber, No Patch* AR0-3941-12 AR0-3941-13
Cert+ Vial, 9 mm, Screw, PP, 300 µL Clear, No Patch** AR0-39S0-12-C AR0-39S0-13-C
Vial, 9 mm, Screw, PP, 1.7 mL Clear, No Patch** — AR0-39P0-13
Vial, 9 mm, Screw, PP, 700 µL Clear, No Patch** — AR0-39P1-13
Vial, 9 mm, Screw, PP, 300 µL Clear, No Patch** — AR0-39P2-13
*51-Expansion glass. **Vial made of Polypropylene.

Phenomenex 37
 Verex ™
Certified Vial Products

12 x 32 mm, 9 mm (9-425) Screw-Top Vials and Kits (cont’d)

Certified PLUS (Cert+) Mass Spec Quality (MSQ) Caps RIGHT CHOICE
MS Quality. Constructed of virgin
for 9 mm Screw-Top Vials polymers, free of siloxanes and
trace contaminants, these caps
• HPLC and LC-MS tested and certified are the cleanest available from
Phenomenex.
• For your most demanding analysis; high sensitive detectors
Certified Plus
MS Quality
• Locked-fit provides a positive physical septa lock into the cap
3
Ordering Information
Description 1000/pk
Cert+ MSQ Cap (pre-assembled), 9 mm, w/ Locked-fit PTFE/Silicone septa, blue AR0-8952-13-M
Cert+ MSQ Cap (pre-assembled), 9 mm, w/ Locked-fit PTFE/Silicone preSlit septa, blue AR0-8972-13-M

Certified PLUS (Cert+) Caps for 9 mm Screw-Top Vials

• Certified ultra-clean
• High-grade, preconditioned raw materials
• Finished product inspected and LC-MS tested
Ordering Information 2
AUTOSAMPLER VIALS | VIALS - SAMPLE HANDLING

Description 1000/pk
Cert+ Cap (pre-assembled), 9 mm, w/ Bonded-in PTFE/Silicone septa, blue AR0-8952-13-C
Cert+ Cap (pre-assembled), 9 mm, w/ Bonded-in PTFE/Silicone preSlit septa, blue AR0-8972-13-C
Cert+ Cap (one-piece), 9 mm PE w/ Starburst preSlit, natural*∆∆ AR0-89P6-13-C
*Cap is one piece, constructed of ultra-pure, medical-grade polyethylene
∆∆
This cap is compatible with all 9 mm screw top vials except AR0-39P2-13

For a more economical alternative,

Bonded-In Caps for 9 mm Screw-Top Vials Press-Fit Caps are available.
Contact your Phenomenex
• Bonded septa caps eliminate costly liner/septa fallout technical consultant or distributor.
• Prevents rework and wasted productivity with perfect-fit septa
• Saves instrument downtime
1
Ordering Information
Description 1000/pk
Cap (pre-assembled), 9 mm, w/ Bonded-in PTFE/Silicone septa, black AR0-8957-13-B
Cap (pre-assembled), 9 mm, w/ Bonded-in PTFE/Silicone septa, blue AR0-8952-13-B
Cap (pre-assembled), 9 mm, w/ Bonded-in PTFE/Silicone septa, natural AR0-8956-13-B
Cap (pre-assembled), 9 mm, w/ Bonded-in PTFE/Silicone septa, red AR0-8951-13-B
Cap (pre-assembled), 9 mm, w/ Bonded-in PTFE/Silicone septa, green AR0-8953-13-B
Cap (pre-assembled), 9 mm, w/ Bonded-in PTFE/Silicone preSlit septa, black AR0-8977-13-B
Cap (pre-assembled), 9 mm, w/ Bonded-in PTFE/Silicone preSlit septa, blue AR0-8972-13-B
Cap (pre-assembled), 9 mm, w/ Bonded-in PTFE/Silicone preSlit septa, grey AR0-8976-13-B
Cap (one-piece), 9 mm PP, molded, natural* AR0-39P9-13
Cap 9 mm Solid Top, Unlined (no Septa), Polypropylene, black AR0-89C7-13
Description 100/pk
Poly/Steel Caps
Poly/Steel Cap (pre-assembled), 9 mm, w/ Bonded-in PTFE/Silicone septa, blue AR0-895P-12-B
Poly/Steel Cap (pre-assembled), 9 mm, w/ Bonded-in PTFE/Silicone preSlit septa, blue AR0-897P-12-B
*Cap is one piece, constructed of ultra-pure, medical-grade polyethylene

Inserts for 9 mm Screw-Top Vials

• For limited volume sampling in 2 mL sample vials
• Virtually no adsorption or pH effects

Ordering Information∆
Description 500/pk 1000/pk
Insert, 6 mm Diameter, Flat Bottom, 450 µL AR0-4610-52 —
Insert, 6 mm Diameter, Flat Bottom, 450 µL, Silanized AR0-4615-52 —
Insert, 6 mm Diameter, Conical Bottom, 350 µL — AR0-4620-13
Insert, 6 mm Diameter, Conical Bottom, w/ bottom spring, 300 µL — AR0-4621-13
Insert, 6 mm Diameter, Conical Bottom, w/ bottom spring, 300 µL, Silanized — AR0-4623-13
Approximate useable volume indicated in μL
∆

38 Phenomenex
Verex ™
Certified Vial Products

12 x 32 mm, 8 mm (8-425) Screw-Top Vials

8 mm Screw-Top Vials, 2.0 mL
• Standard-neck vials for Agilent® and Shimadzu® autosamplers
• 33-Expansion clear glass (USP Type 1 borosilicate, Class A), 51A amber glass
Ordering Information
Description 1000/pk
Vial, 8 mm Screw, 2 mL Clear, No Patch AR0-3800-13
Vial, 8 mm Screw, 2 mL Clear, w/ Patch AR0-3810-13
Vial, 8 mm Screw, 2 mL Amber, No Patch AR0-3801-13
Vial, 8 mm Screw, 2 mL Amber, w/ Patch AR0-3811-13

Caps / Closures
for 8 mm Screw-Top Vials
• Prevent rework and wasted productivity with perfect fit bonded-in septa
• Fewer trace contaminants for higher sensitivity work
Ordering Information

SAMPLE HANDLING - VIALS | AUTOSAMPLER VIALS

Description 1000/pk
Cap (pre-assembled), 8-425, w/ Bonded-in PTFE/Silicone septa, black* AR0-8857-13-B
Cap (pre-assembled), 8-425, w/ Bonded-in PTFE/Silicone preSlit septa, black* AR0-8877-13-B
Cap (pre-assembled), 8-425, w/ PTFE/Silicone septa, black** AR0-8857-13
Cap (pre-assembled), 8-425, w/ PTFE/Silicone preSlit septa, black** AR0-8877-13
Cap (pre-assembled), 8-425, w/ PTFE/Silicone septa, w/ flange, yellow AR0-8834-13
Cap (pre-assembled), 8-425, w/ PTFE/Silicone/PTFE septa, black** AR0-8867-13
Cap (pre-assembled), 8-425, w/ Open-top cap (w/o septa), black AR0-8897-13
*-B = Bonded-in Septa
**Press-Fit-Septa

Septa Need help matching your current

vials and caps to Verex? Visit:
for 8 mm Screw Caps www.phenomenex.com/VialFinder
• Preconditioned and tested
• For caps / closures without septa (AR0-8897-13)
Ordering Information
Description 1000/pk
Septa, 8 mm Diameter, PTFE/Silicone 0.060 in AR0-6853-13
Septa, 8 mm Diameter, PTFE/Silicone/PTFE 0.060 in AR0-6863-13
Septa, 8 mm Diameter, PTFE 0.010 in AR0-6817-13

Inserts
for 8 mm Screw-Top Vials
• For limited volume sampling
• Transforms full capacity vials to micro sample vials

Ordering Information∆
Description 500/pk 1000/pk
Insert, 5 mm Diameter, Flat Bottom, 200 µL AR0-4510-52 AR0-4510-13
Insert, 5 mm Diameter, Conical Bottom, 100 µL — AR0-4520-13
Insert, 5 mm Diameter, Conical Bottom, w/ bottom spring, 175 µL — AR0-4521-13
Approximate useable volume indicated in μL
∆

Phenomenex 39
 Verex ™
Certified Vial Products

12 x 32 mm, 10 mm (10-425) Screw-Top Vials and Kits

Convenience Kits
10 mm Screw-Top Vials and Caps with Bonded-In Septa
• Available in assembled ready to use kits
• Offered with silanization
Ordering Information
Description 100/pk 1000/pk
Vial Kit, 10 mm, 2 mL Clear, No Patch + PTFE/Silicone, preSlit — AR0-9003-13
Vial Kit, 10 mm, 2 mL Clear, No Patch + PTFE/Silicone, Silanized AR0-9005-12 —
Vial Kit, 10 mm, 2 mL Clear, No Patch + PTFE/Silicone, preSlit, Silanized AR0-9006-12 —
Vial Kit, 10 mm, 2 mL Clear, w/ Patch + PTFE/Silicone* — AR0-9021-13-A
Vial Kit, 10 mm, 2 mL Amber, w/ Patch + PTFE/Silicone* — AR0-9022-13-A
*-A = Assembled

10 mm Screw-Top Vials, 2.0 mL

• Easy-to-fill wide-neck vials
Ordering Information
Description 1000/pk
AUTOSAMPLER VIALS | VIALS - SAMPLE HANDLING

Vial, 10 mm Screw, 2 mL Clear, No Patch AR0-3000-13

Vial, 10 mm Screw, 2 mL Clear, w/ Patch AR0-3010-13
Vial, 10 mm Screw, 2 mL Clear, No Patch, Silanized AR0-3003-13
Vial, 10 mm Screw, 2 mL Amber, No Patch AR0-3001-13
Vial, 10 mm Screw, 2 mL Amber, w/ Patch AR0-3011-13

Limited Volume Specialty 10 mm Screw-Top Vials

• Microsampling Qsert and center-draining vial
• Extremely low residual volume
Ordering Information
Description 100/pk 1000/pk
Vial, 10 mm Screw, µVial i3 (Qsert) Clear, No Patch AR0-3020-12 AR0-3020-13
Vial, 10 mm Screw, µVial i3 (Qsert) Amber, No Patch AR0-3021-12 AR0-3021-13
Vial, 10 mm Screw, v-Vial, Clear, No Patch AR0-3040-12 AR0-3040-13

Caps / Closures
for 10 mm Screw-Top Vials
• High quality preconditioned septa
• Bonded-in or press-fit
• Prevent rework and wasted productivity with perfect fit bonded-in septa
Ordering Information
Description 1000/pk
Cap (pre-assembled), 10-425, w/ Bonded-in PTFE/Silicone septa, black* AR0-8057-13-B
Cap (pre-assembled), 10-425, w/ Bonded-in PTFE/Silicone preSlit septa, black* AR0-8077-13-B
Cap (pre-assembled), 10-425, w/ PTFE/Silicone septa, black** AR0-8057-13
Cap (pre-assembled), 10-425, w/ PTFE/Silicone preSlit septa, black** AR0-8077-13
*-B = Bonded-in Septa
**Press-fit Septa

Inserts
for 10 mm Screw-Top Vials
• For limited volume sampling
Ordering Information∆
Description 500/pk 1000/pk
Insert, 6 mm Diameter, Flat Bottom, 450 µL AR0-4610-52 —
Insert, 6 mm Diameter, Flat Bottom, 450 µL, Silanized AR0-4615-52 —
Insert, 6 mm Diameter, Conical Bottom, 350 µL — AR0-4620-13
Insert, 6 mm Diameter, Conical Bottom, w/ bottom spring, 300 µL — AR0-4621-13
Insert, 6 mm Diameter, Conical Bottom w/ bottom spring, 300 µL, Silanized — AR0-4623-13
Approximate useable volume indicated in μL
∆

40 Phenomenex
Verex ™
Certified Vial Products

15 x 45 mm, 13 mm (13-425) Screw-Top Vials and Kits

Convenience Kits – 13 mm Screw-Top Vials and Caps
• Original Waters® 4 mL WISP™ autosampler vial
• General purpose sample / standard storage vial
Ordering Information
Description 1000/pk
Vial Kit, 4 mL Clear w/ Patch + screw caps 13-425, PTFE/Silicone*∆ AR0-9321-13-A
Vial Kit, 4 mL Amber w/Patch + screw caps 13-425, PTFE/Silicone* AR0-9422-13-A
Vial Kit, 4 mL Amber w/ Patch + screw caps 13-425, PTFE 0.01 in.* AR0-9392-13-A
*-A = Assembled. ∆
= Bonded-In Septa

13 mm Screw-Top Vials, 4.0 mL

Ordering Information
Description 1000/pk
Vial, 4 mL Screw Clear, No Patch AR0-3300-13
Vial, 4 mL Screw Clear, w/ Patch AR0-3310-13
Vial, 4 mL Screw Amber, No Patch AR0-3301-13

SAMPLE HANDLING - VIALS | AUTOSAMPLER VIALS

Limited Volume Specialty 13 mm Screw-Top Vials
• Center-draining bottom for low-volume samples
Ordering Information
Description 100/pk 1000/pk
Vial, 4 mL Screw High Recovery (CD) Clear, No Patch AR0-3370-12 AR0-3370-13

Caps / Closures
for 13 mm Screw Top Vials
• High quality, pre-conditioned septa
• Available with bonded-in septa
Ordering Information
Description 1000/pk
Cap (pre-assembled), 13-425, w/ Bonded-in PTFE/Silicone septa, black* AR0-8357-13-B
Cap (pre-assembled), 13-425, w/ PTFE/Silicone septa, black∆ ∆ AR0-8357-13
*-B = Bonded-in Septa. ∆ ∆ = Press-fit

Shell Vials
• Original 1 mL and 4 mL shell vials for Waters® 96- and
48-position autosampler trays
• Kits include high-quality borosilicate glass vials with
convenient push-in polyethylene cap

Convenience Kits – Shell Vials, 1.0 and 4.0 mL

Ordering Information
Description 1000/pk
Shell Vial Kit, 8 x 40 mm, 1 mL Flat Bottom, Clear + PE Cap AR0-3110-13
Shell Vial Kit, 15 x 45 mm, 4 mL Flat Bottom, Clear + PE Cap AR0-3170-13

51-Expansion glass.

Convenience Kits – Certified PLUS (Cert+) Shell Vials, 1.0 mL

Ordering Information
Description 200/pk
Cert+ Shell Vial Kit, 8 x 40 mm, 1 mL Flat Bottom, Clear + PE Cap AR0-3110-22-C
Cert+ Shell Vial Kit, 8 x 40 mm, 1 mL Flat Bottom, Amber + PE Cap AR0-3111-22-C

Phenomenex 41
 Verex ™
Certified Vial Products

Headspace Vials
Screw- and Crimp-Top Headspace Vials
• 10 and 20 mL screw- or crimp-top, with round or flat bottom
• Uniform glass thickness ensures even heating
• Lot traceable
Ordering Information
Description 1000/pk
Crimp-Top
Headspace Vial, 22 x 38 mm, 6 mL Beveled Edge, Flat Bottom, Clear, No Patch AR0-32F0-13
Headspace Vial, 22 x 38 mm, 6 mL Square Rim, Flat Bottom, Clear, No Patch AR0-32D0-13
Headspace Vial, 22 x 38 mm, 6 mL Beveled Edge, Round Bottom, Clear, No Patch AR0-32G0-13
Headspace Vial, 23 x 46 mm, 10 mL Beveled Edge, Flat Bottom, Clear, No Patch AR0-3220-13
Headspace Vial, 23 x 46 mm, 10 mL Square Rim, Flat Bottom, Clear, No Patch AR0-32A0-13
Headspace Vial, 23 x 46 mm, 10 mL Beveled Edge, Round Bottom, Clear, No Patch AR0-3230-13
Headspace Vial, 23 x 75 mm, 20 mL Beveled Edge, Flat Bottom, Clear, No Patch AR0-3260-13
Headspace Vial, 23 x 75 mm, 20 mL Beveled Edge, Flat Bottom., Clear, No Patch, Silanized AR0-3263-13
Headspace Vial, 23 x 75 mm, 20 mL Square Rim, Flat Bottom, Clear, No Patch AR0-3290-13
Headspace Vial, 23 x 75 mm, 20 mL Beveled Edge, Round Bottom, Clear, No Patch AR0-3270-13
AUTOSAMPLER VIALS | VIALS - SAMPLE HANDLING

Screw-Top
Headspace Vial, 23 x 46 mm, 10 mL 18 mm Screw, Round Bottom, Clear, No Patch AR0-32H0-13
Headspace Vial, 23 x 46 mm, 10 mL 18 mm Screw, Round Bottom, Amber, No Patch AR0-32H1-13
Headspace Vial, 23 x 75 mm, 20 mL 18 mm Screw, Round Bottom, Clear, No Patch AR0-3280-13
Headspace Vial, 23 x 75 mm, 20 mL 18 mm Screw, Round Bottom, Amber, No Patch AR0-3281-13

Autosampler Compatibility
Flat Bottom: HP / Agilent, Carlo Erba, Shimadzu
Round Bottom: PerkinElmer, Tekmar, LEAP Technologies, Varian

Headspace Screw- and Crimp-Top Seals / Closures

• Variety of styles for any application
• Magnetic and pressure-release caps available

Ordering Information
Description 1000/pk
Crimp-Top
Seal, 20 mm Diameter, PTFE/Gray Butyl Rubber, magnetic cap AR0-52C5-13
Seal, 20 mm Diameter, PTFE/Butyl Rubber Pharmafix Molded Septum, silver AR0-52D0-13
Seal, 20 mm Diameter, PTFE/Butyl Rubber Pressure Release, Pharmafix Molded Septum, silver AR0-52B0-13
Seal, 20 mm Diameter, PTFE/Silicone, magnetic cap AR0-5255-13
Seal, 20 mm Diameter, PTFE/Silicone, silver AR0-5250-13
Seal, 20 mm Diameter, PTFE/Silicone Pressure Release, silver AR0-5220-13
Screw-Top
Screw Cap, 18 mm, Magnetic, Silver, PTFE/Butyl Rubber septa (red/grey) AR0-814M-13
Screw Cap, 18 mm, Magnetic, Silver, PTFE/Silicone septa (red/white) AR0-815M-13
Screw Cap, 18 mm, Magnetic, Silver, PTFE/Silicone septa (blue/white) AR0-81AM-13
Screw Cap, 18 mm, Magnetic, Silver, PTFE/Silicone septa (white/translucent blue) AR0-81BM-13

42 Phenomenex
Verex ™
Certified Vial Products

VOA / ASE Assembled Vial Kits and Storage Vial Kits

• Convenience and assembled kits include vials with matching seals / closures
• Clear or amber, with open top or closed caps
• PTFE (Teflon®) faced 0.125 in. silicone septa or PTFE with foam urethane backing
• Lot numbered for traceability

Ordering Information
Description 100/pk 1000/pk
Storage Vial Kits 0
Vial Kit, Storage, 20 mL Screw, Clear w/ Caps 24-400 white PTFE/Foam Urethane liner, closed top AR0-9559-12 AR0-9559-13
Vial Kit, Storage, 20 mL Screw, Amber w/ Caps 24-400 white PTFE/Foam Urethane liner, closed top AR0-9551-12 AR0-9551-13
Vial Kit, Storage, 40 mL Screw, Clear w/ Caps 24-400 white PTFE/Foam Urethane, closed top AR0-9542-12 AR0-9542-13
Vial Kit, Storage, 40 mL Screw, Amber w/Caps 24-400 white PTFE/Foam Urethane, closed top AR0-9543-12 AR0-9543-13
VOA/ASE Vial Kits 0
Vial Kit, VOA/ASE, 40 mL Screw, Clear w/Caps 24-400 white PTFE/Silicone, open top AR0-9540-12 AR0-9540-13
Vial Kit, VOA/ASE, 40 mL Screw, Clear w/Caps 24-400 white PTFE/Silicone, (assembled), open top AR0-9540-12-A AR0-9540-13-A
Vial Kit, VOA/ASE, 40 mL Screw, Amber w/ Caps 24-400 white PTFE/Silicone, open top AR0-9541-12 —
Vial Kit, VOA/ASE, 20 mL Screw, Clear w/ Caps 24-400 white PTFE/Silicone (assembled)*, open top AR0-9531-12-E AR0-9531-13-E
* EPA certified Class 100 vials, caps, and septa are assembled, without processing.

SAMPLE HANDLING - VIALS | AUTOSAMPLER VIALS

ASE = Dionex Accelerated Solvent Extractor for pestcide analysis.

VOA / ASE Vials and Caps (Separate)

Ordering Information
Description 200/pk
Vials
Vial, VOA/ASE, 40 mL Screw, Clear, 24-400 Threads (No Cap) AR0-35V0-22
Vial, VOA/ASE, 40 mL Screw, Amber, 24-400 Threads (No Cap) AR0-35V1-22
Description 1000/pk
Caps
Cap (pre-assembled), Screw (24-414), w/ Bonded-in PTFE/Silicone septa, white AR0-8557-13-B

Learn More. For additional product

selection and detailed information visit:
www.phenomenex.com/Verex

Protect your HPLC/UHPLC and GC columns

and equipment with Phenex Syringe Filters

Filtering your sample helps prevent column

and frit blockage, undue wear on detectors,
pumps, valves, injector seals, and abnormally
high operating pressures. Non-filtered samples
can also lead to non-reproducible results and
significant instrument downtime.

See page 8 or Visit:

www.phenomenex.com/SFfinder

Phenomenex 43
 Verex -EU Vial Products ™

Offered by Phenomenex in Europe only.

Leading in Quality, Delivery, and Support 100 % Defect-Free

From start to finish, Verex-EU vial and cap products are manu- No product is placed in inventory without passing cleanliness and
factured to provide high quality, state-of-the-art solutions for your performance testing for consistent results.
most challenging, sensitive applications.
Clear and Amber Glass
Certified Verex-EU vials use 51A-Expansion glass (USP Type 1 borosilicate,
The highest quality materials combine with tightly controlled manu- Class B) for both clear and amber glass vials.
facturing processes to produce uniform, trouble-free products you
Learn More. For additional product
can rely on.
selection and detailed information visit:
www.phenomenex.com/Verex

12 x 32 mm, 11 mm Crimp-Top Vials and Seals

Ordering Information
Description 100/pk
Verex-EU Vial, Crimp, 2 mL Clear, w/ Patch AR1-3710-12
Verex-EU Vial, Crimp, 2 mL Amber, w/ Patch AR1-3711-12
AUTOSAMPLER VIALS | VIALS - SAMPLE HANDLING

Seals / Closures for Crimp-Top Vials

Ordering Information
Description 100/pk
Seal, 11 mm Diameter, Crimp, PTFE/Silicone, silver AR0-5780-12
Seal, 11 mm Diameter, Crimp, PTFE/Silicone/PTFE, silver AR0-5760-12
Seal, 11 mm Diameter, Crimp, PTFE/Rubber, silver AR1-5740-12
Seal, 11 mm Diameter, Crimp, PTFE/Rubber, blue AR1-5742-12
Seal, 11 mm Diameter, Crimp, PTFE/Rubber, red AR1-5741-12
Seal, 11 mm Diameter, Crimp, PTFE/Rubber, green AR0-5743-12
Seal, 11 mm Diameter, Crimp, PTFE/Rubber, gold AR0-574G-12
Seal, 11 mm Diameter, Crimp, PTFE, silver AR0-5710-12

12 x 32 mm, 11 mm Snap-Top Vials and Caps

Ordering Information
Description 100/pk
Verex-EU Vial, Snap, 2 mL Clear, w/ Patch AR1-3610-12
Verex-EU Vial, Snap, 2 mL Amber, w/ Patch AR1-3611-12

Seals / Closures for Snap-Top Vials

Ordering Information
Description 100/pk Price
Seal, 11 mm Diameter, Snap, PTFE/Silicone, blue AR1-5652-12
Seal, 11 mm Diameter, Snap, PTFE/Silicone, natural AR1-5656-12
Seal, 11 mm Diameter, Snap, PTFE/Silicone preSlit, blue AR1-5672-12
Seal, 11 mm Diameter, Snap, PTFE/Silicone preSlit, natural AR0-5676-12
Seal, 11 mm Diameter, Snap, PTFE/Rubber, natural AR0-5646-12
Seal, 11 mm Diameter, Snap, PTFE, blue AR0-5612-12

12 x 32 mm, 13 mm (13-425) Screw-Top Vials and Caps

Ordering Information
Description 100/pk
Verex-EU Vial, 4 mL Screw Clear, w/ Patch AR1-3310-12

Caps / Closures
for 13 mm Screw Top Vials
Ordering Information
Description 100/pk
Cap (pre-assembled), 13-425, w/ Bonded-in PTFE/Silicone septa, black  AR0-8357-12-B
Cap (pre-assembled), 13-425, w/ PTFE/Silicone septa, black  AR0-8357-12

-B = Bonded-in Septa. 
Press-Fit Septa

44 Phenomenex
Verex -EU Vial Products ™

Offered by Phenomenex in Europe only. RIGHT CHOICE

MS Quality. Constructed of virgin
polymers, free of siloxanes and
trace contaminants, these caps
are the cleanest available from
Phenomenex.
Certified Plus
MS Quality

12 x 32 mm, 9 mm (9-425) Screw-Top Vials and Caps

Ordering Information
Description 100/pk
Verex-EU Vial, 9 mm Screw, 2 mL Clear, w/ Patch AR1-3910-12
Verex-EU Vial, 9 mm Screw, 2 mL Amber, w/ Patch AR1-3911-12

Caps
for 9 mm Screw-Top Vials
Ordering Information
Description 100/pk
Certified PLUS (Cert+) Mass Spec Quality (MSQ) Caps for 9-425 Screw-Top Vials
Cert+ MSQ Cap (pre-assembled), 9 mm, w/ Locked-fit PTFE/Silicone septa, blue AR0-8952-12-M
Cert+ MSQ Cap (pre-assembled), 9 mm, w/ Locked-fit PTFE/Silicone preSlit septa, blue AR0-8972-12-M 3
Certified PLUS (Cert+) Caps for 9-425 Screw-Top Vials

SAMPLE HANDLING - VIALS | AUTOSAMPLER VIALS

Cert+ Cap (pre-assembled), 9 mm, w/ Bonded-in PTFE/Silicone septa, blue AR0-8952-12-C
Cert+ Cap (pre-assembled), 9 mm, w/ Bonded-in PTFE/Silicone preSlit septa, blue
Cert+ Cap (one-piece), 9 mm PE w/ Starburst preSlit, natural*
AR0-8972-12-C
AR0-89P6-12-C
2
Bonded-In Caps for 9-425 Screw-Top Vials
Cap (pre-assembled), 9 mm, w/ Bonded-in PTFE/Silicone septa, black AR0-8957-12-B
Cap (pre-assembled), 9 mm, w/ Bonded-in PTFE/Silicone septa, blue AR1-8952-12-B
Cap (pre-assembled), 9 mm, w/ Bonded-in PTFE/Silicone septa, natural AR1-8956-12-B
Cap (pre-assembled), 9 mm, w/ Bonded-in PTFE/Silicone septa, red AR1-8951-12-B 1
Cap (pre-assembled), 9 mm, w/ Bonded-in PTFE/Silicone preSlit septa, black AR0-8977-12-B
Cap (pre-assembled), 9 mm, w/ Bonded-in PTFE/Silicone preSlit septa, blue AR0-8972-12-B

* Cap is one piece, constructed of ultra-pure, medical-grade polyethylene

12 x 32 mm, 8 mm (8-425) Screw-Top Vials and Caps

Ordering Information
Description 100/pk
Verex-EU Vial, 8 mm Screw, 2 mL Clear, w/ Patch AR1-3810-12
Verex-EU Vial, 8 mm Screw, 2 mL Amber, w/ Patch AR1-3811-12

Caps / Closures
for 8 mm Screw-Top Vials
Ordering Information
Description 100/pk
Cap (pre-assembled), 8-425, w/ Bonded-in PTFE/Silicone septa, black  AR0-8857-12-B
Cap (pre-assembled), 8-425, w/ Bonded-in PTFE/Silicone preSlit septa, black  AR0-8877-12-B
Cap (pre-assembled), 8-425, w/ PTFE/Silicone septa, black  AR0-8857-12
Cap (pre-assembled), 8-425, w/ PTFE/Silicone preSlit septa, black  AR0-8877-12
Cap (pre-assembled), 8-425, w/ PTFE/Silicone/PTFE septa, black  AR0-8867-12
Cap (pre-assembled), 8-425, w/ Open-top cap (w/o septa) AR0-8897-12

-B = Bonded-in Septa. 
Press-Fit Septa

Need help matching your current

Septa vials and caps to Verex? Visit:
www.phenomenex.com/VialFinder
for 8 mm Screw Caps
Ordering Information
Description 100/pk
Septa, 8 mm Diameter, PTFE/Silicone 0.060 in. AR0-6853-12
Septa, 8 mm Diameter, PTFE 0.010 in. AR0-6817-12

Phenomenex 45
 Verex -EU Vial Products ™

Offered by Phenomenex in Europe only.

12 x 32 mm, 10 mm (10-425) Screw-Top Vials and Caps
Ordering Information
Description 100/pk
Verex-EU Vial, 10 mm Screw, 2 mL Clear, w/ Patch AR1-3010-12
Verex-EU Vial, 10 mm Screw, 2 mL Amber, w/ Patch AR1-3011-12

Caps/ Closures
for 10 mm Screw-Top Vials
Ordering Information
Description 100/pk
Cap (pre-assembled), 10-425, w/ Bonded-in PTFE/Silicone septa, black  AR0-8057-12-B
Cap (pre-assembled), 10-425, w/ Bonded-in PTFE/Silicone preSlit septa, black  AR0-8077-12-B
Cap (pre-assembled), 10-425, w/ PTFE/Silicone septa, black  AR0-8057-12
Cap (pre-assembled), 10-425, w/ PTFE/Silicone preSlit septa, black  AR0-8077-12

-B = Bonded-in Septa. 
Press-Fit Septa

Headspace Vials and Caps

AUTOSAMPLER VIALS | VIALS - SAMPLE HANDLING

Ordering Information
Description 100/pk Price
Verex-EU Headspace Vial, 23 x 46 mm, 10 mL Beveled Edge, Flat Bottom, Clear, No Patch AR1-3220-12
Verex-EU Headspace Vial, 23 x 46 mm, 10 mL Beveled Edge, Round Bottom, Clear, No Patch AR1-3230-12
Verex-EU Headspace Vial, 23 x 75 mm, 20 mL Beveled Edge, Flat Bottom, Clear, No Patch AR1-3260-12
Verex-EU Headspace Vial, 23 x 75 mm, 20 mL Beveled Edge, Round Bottom, Clear, No Patch AR1-3270-12

Headspace Crimp-Top Seals / Closures

Ordering Information
Description 100/pk
Seal, 20 mm Diameter, PTFE/Gray Butyl Rubber, magnetic cap AR0-52C5-12
Seal, 20 mm Diameter, PTFE/Gray Butyl Rubber, Pressure Release, silver AR0-52A0-12
Seal, 20 mm Diameter, PTFE/Butyl Rubber Pharmafix Molded Septum, silver AR0-52D0-12
Seal, 20 mm Diameter, PTFE/Butyl Rubber Pressure Release, Pharmafix Molded Septum, silver AR0-52B0-12
Seal, 20 mm Diameter, PTFE/Silicone, magnetic cap AR0-5255-12
Seal, 20 mm Diameter, PTFE/Silicone, silver AR1-5250-12
Seal, 20 mm Diameter, PTFE/Silicone Pressure Release, silver AR0-5220-12

VOA Vials and Caps

Ordering Information
Description 100/pk Price
Verex-EU Vial 40 mL Clear 24-414 Screw 28 x 95 mm AR1-35V0-12
Verex-EU Vial 60 mL Clear 24-414 Screw 28 x 140 mm AR1-35A0-12
Verex-EU Vial 60 mL Amber 24-414 Screw 28 x 140 mm AR1-35A1-12

VOA Vial Caps

Ordering Information
Description 100/pk Price
Verex Cap (pre-assembled), 24-414, w/ Bonded-in PTFE/Silicone septa, white AR0-8557-12-B

For Vial Inserts for 8 mm Screw-Top Vials, see p. 39

For Vial Inserts for 9 mm Screw-Top Vials, see p. 38
For Vial Inserts for 10 mm Screw-Top Vials, see p. 40

46 Phenomenex
Verex -IN Vial Products ™

Offered by Phenomenex in India only.

Leading in Quality, Delivery, and Support 100 % Defect-Free

Verex-IN products are engineered to exact specification because Machine vision cameras and gauges inspect critical dimensions
uniform, high-purity vials are essential to accurate, reproducible and tolerances to produce uniform, defect-free products.
chromatographic results.

Certified
High quality materials combined with tightly controlled manufac- Learn More. For additional product
turing processes produce uniform, trouble-free products you can selection and detailed information visit:
rely on. www.phenomenex.com/Verex

12 x 32 mm, 9 mm (9-425) Screw-Top Vials and Caps

Ordering Information
Description 100/pk
Verex-IN Vial, 9 mm Screw, 2 mL Clear, w/ Patch AR4-3910-12

SAMPLE HANDLING - VIALS | AUTOSAMPLER VIALS

Caps
for 9 mm Screw-Top Vials
Ordering Information
Description 100/pk
Cap (pre-assembled), 9 mm, w/ Bonded-in PTFE/Silicone septa, black AR4-8957-12-B
Cap (pre-assembled), 9 mm, w/ Bonded-in PTFE/Silicone preSlit septa, black AR4-8977-12-B

Need Additional Cap Colors?

Caps
for 9 mm Screw-Top Vials
Ordering Information
Description 100/pk
Certified PLUS (Cert+) Mass Spec Quality (MSQ) Caps for 9-425 Screw-Top Vials
Cert+ MSQ Cap (pre-assembled), 9 mm, w/ Locked-fit PTFE/Silicone septa, blue AR0-8952-12-M
Cert+ MSQ Cap (pre-assembled), 9 mm, w/ Locked-fit PTFE/Silicone preSlit septa, blue AR0-8972-12-M 3
Certified PLUS (Cert+) Caps for 9-425 Screw-Top Vials
Cert+ Cap (pre-assembled), 9 mm, w/ Bonded-in PTFE/Silicone septa, blue AR0-8952-12-C
Cert+ Cap (pre-assembled), 9 mm, w/ Bonded-in PTFE/Silicone preSlit septa, blue
Cert+ Cap (one-piece), 9 mm PE w/ Starburst preSlit, natural*
AR0-8972-12-C
AR0-89P6-12-C
2
Bonded-In Caps for 9-425 Screw-Top Vials
Cap (pre-assembled), 9 mm, w/ Bonded-in PTFE/Silicone septa, blue AR1-8952-12-B
Cap (pre-assembled), 9 mm, w/ Bonded-in PTFE/Silicone septa, natural AR1-8956-12-B
Cap (pre-assembled), 9 mm, w/ Bonded-in PTFE/Silicone septa, red AR1-8951-12-B
Cap (pre-assembled), 9 mm, w/ Bonded-in PTFE/Silicone preSlit septa, blue AR0-8972-12-B 1
* Cap is one piece, constructed of ultra-pure, medical-grade polyethylene

For Vial Inserts for 9 mm Screw-Top Vials, see p. 38

Phenomenex 47
 Verex ™
Certified Vial Products

Make the Switch to Verex!

Easily Find Your Vial Online

2 Easy Steps:

1 1 Enter your current vial or

cap part number

2
2 Order the recommended
Verex part number

Visit:
www.phenomenex.com/VialFinder

Download FREE Verex Download FREE Verex

Autosampler Compatibility Chart Vials and Caps Poster Guide

Quick reference chart

for selecting Verex
vial products for your
specific autosampler.

Visit: Visit:
www.phenomenex.com/verex www.phenomenex.com/verex

48 Phenomenex
 Sample Preparation

49 - 86

Sample Preparation Solutions and Formats .............................. 50

Novum and Novum PRO Simplified
Liquid Extraction (SLE)........................................................51-53

“
Strata DE Diatomaceous Earth SLE ......................................54-55
β-Gone β-Glucuronidase Removal Products ............................. 56
Strata-X is an excellent all-around
Impact Protein Precipitation Plates ............................................ 57
sorbent and we obtain high breakthrough
Phree Phospholipid Removal Solutions ................................58-59

”
volumes necessary to improve method
Strata-X PRO Rapid Solid Phase Extraction Solution................ 60 quantitation limits.
Strata-X Polymeric Solid Phase Extraction (SPE) .................61-69
Strata SPE ...............................................................................70-77 Pedro A. Segura
Biozen Biological Sample Clean-up Université de Montreal
MagBeads ................................................................................ 78 The opinions stated herein are solely those of the speaker
and not necessarily those of any company or organization.
N-Glycan Clean-up .................................................................. 78
Sample Preparation Accessories
Presston 1000 Positive Pressure Manifold ............................. 79
Vacuum Manifolds and Collection Plates ..........................80-82
roQ QuEChERS Kits ...............................................................83-85
Sample Preparation Resources .................................................. 86
 Sample Preparation
Choose Your Sample Preparation Solution

A mechanical or physical operation which is used for the separa-

Filtration tion of solids from fluids by interposing a medium through which
Certified Filter Vials only the fluid can pass.

Protein  Proteinaceous samples require a protein precipitation step to

promote protein aggregation which allows their removal from t he
Precipitation Rapid Protein Precipitation solution/sample.

β-Glucuronidase A β-Glucuronidase enzyme removal method to clean-up

hydrolyzed urine from samples in less than 1 minute, ideal for
Removal β-Glucuronidase Removal Products rapid drug testing.

Phospholipid Removal Biological samples require the removal of endogenous

+ phospholipids and proteins as they are a primary source of ion
Protein Precipitation Phospholipid Removal Solutions suppression and resulting matrix effects.

A streamlined approach that makes it easier and less expensive

for analytical chemists to examine residues in food. The name is
QuEChERS a portmanteau word formed from “Quick, Easy, Cheap, Effective,
Rugged, and Safe”.

Supported Liquid Extraction (SLE) is a FASTER, EASIER,

Supported and MORE RELIABLE way to perform liquid-liquid extraction.
Unwanted interferences can be removed such as proteins, salts
Liquid Extraction Solid Phase Extraction simplified liquid extraction
and phospholipids.

A separation process that is used to remove compounds from

SAMPLE PREPARATION SOLUTIONS / FORMATS | SAMPLE PREPARATION

Solid Phase a mixture, based on their physical and chemical properties.

Analytical laboratories use solid phase extraction to concentrate
Extraction
A Rapid Solid Phase Extraction Solution
Solid Phase Extraction Polymeric SPE
and purify samples for analysis from a wide variety of matrices.

Paramagnetic beads used to capture streptavidin or other target

Immunocapture analytes in order to perform a clean-up of biologics.

Available Formats
Giga™ Tubes On-line Extraction
96-Well Plates Microelution Plates 1, 3, and 6 mL Tubes (12 mL - 150 mL) Cartridge Bulk Sorbent
Strata-X PRO SPE X X X
Strata-X Polymeric SPE X X X X X X
Strata Traditional SPE X X X X X
Novum SLE X X
Strata DE SLE X X
Phree Phospholipid Removal Solutions X X
Impact Protein Precipitation Plates X
β-Gone β-Glucuronidase Removal X X

96-Well Plates Microelution Plates 1, 3, and 6 mL Tubes Giga Tubes On-line Extraction Bulk Sorbent
(12 mL - 150 mL) Columns and Cartridges

Don’t see the format you want? Contact For Sepra™ Bulk Sorbent Material Characteristics
Phenomenex or your local Phenomenex and Ordering Information, see p. 401
distributor for custom packed SPE phases

50 Phenomenex
Novum  Simplified Liquid Extraction (SLE)
™
simplified liquid extraction

Patent Pending
Faster, Easier, and More
Reliable than Liquid-Liquid
Extraction
A Simplified Liquid Extraction
• Avoid inferior results due to emulsions Novum SLE will instantly increase your throughput by eliminating
• Eliminate interferences from your samples time consuming steps and reducing the risk of analyte loss. If fur-
ther time savings are necessary, Novum SLE can be easily auto-
• Increase throughput with automatable formats
mated for rapid, hands free sample cleanup.

Slow and Laborious Fast and Easy

1. Dilute sample 1:1 with 1. Dilute sample 1:1 with buffer or water
buffer or water and add and load onto Novum SLE sorbent using
extraction solvent 2–15 seconds of vacuum

novum
simplified liquid extraction
novum
simplified liquid extraction
2. Mix for 10 minutes Traditional Novum Simplified
Liquid-Liquid Extraction1 Liquid Extraction (SLE)
Estimated Time Required 2. Wait 5 minutes Estimated Time Required
= 25 minutes = <15 minutes
3. Apply elution solvent and allow to
3. Centrifuge for 10 minutes elute via gravity. Complete elution
with 10 seconds of vacuum.

SAMPLE PREPARATION
• Rapid, automatable
4. Pour off or freeze supernatant
method for high-
novum
simplified liquid extraction

throughput cleanup
novum
simplified liquid extraction

• Stop worrying
about analyte loss
due to emulsions
1. Russell Grant, Matthew Crawford, Brian Rappold, and Stacy Dee. Errors in Bioanalysis Due to

|
Phospholipids – Definitive Measurement, Mechanism, and Management. ASMS 2011.

NOVUM SLE
Consistent Cleanup from Lot-to-Lot
As a unique, synthetic SLE sorbent you can expect Novum to provide reliable, more consistent
cleanup from lot-to-lot as compared to traditional diatomaceous earth SLE.

Lot-to-Lot Phospholipid Breakthrough: Novum SLE vs. Biotage® ISOLUTE® SLE

0.010 % 100 %
90 % Biotage ISOLUTE SLE+* lot #13165345SA
Novum Lot 1
0.008 % Novum Lot 2 80 % Biotage ISOLUTE SLE+* lot #12161236TA

70 %
0.006 %
Consistently remove 60 %
> 99.9 % of phospholipids
50 %
0.004 % 40 %
30 %
0.002 % 20 %
10 %
0.000 % 0%
Lyso 1 Lyso 2 PC 1 PC 2 PC 4 Lyso 1 Lyso 2 PC 1 PC 2 PC 4

Lyso 1: 1-Palmitoyl-2-OH-sn-glycero-phosphocholine (m/z 496-184)

Lyso 2: 1-Oleoyl-2-OH-sn-glycero-phosphocholine (m/z 522-184)
PC 1: 1-Palmitoyl-2-Oleoyl-sn-glycero-phosphocholine (m/z 761-184)
PC 2: 1-Stearoyl-2-Lindoleoyl-sn-glycero-phosphocholine (m/z 787-184)
For buffer and elution solvent recommendations, PC 4: 1-Oleoyl-2-Lindoleoyl-sn-glycero-phosphocholine (m/z 784-184)
technical notes, demonstration videos, and more, visit:
www.phenomenex.com/Novum Plasma extractions were performed using 200 µL plates and ethyl acetate as an elution solvent.
The recommended protocol provided with each product was followed. Comparative separations
may not be representative of all applications.

*Phenomenex is in no way affiliated with Biotage.

Phenomenex 51
 Novum  Simplified Liquid Extraction (SLE) ™

Patent Pending

Get Down to the Lowest Extraction Levels

with Novum PRO SLE
Offers the same reliable synthetic sorbent as Novum with addi-
tional clean manufacturing steps to reach low levels of detection for
sensitive MS applications, with the same quality reproducibility for
high-throughput samples.

• Specific manufacture capabilities to improve matrix factor

response and reduce noisy baselines for low level testing of
biological samples
• API 6500+ fit for purpose testing to ensure clean baseline
with each batch
• Available in both MINI and MAX 96-well plate formats for
high-throughput applications

Low Level Detection

• Applications that require low levels of detection and
sensitivity can now be met by Novum PRO SLE
10 pg/mL (LLOQ) Estradiol (E2)
NOVUM PRO SLE | SAMPLE PREPARATION

5 pg/mL (LLOQ) Estriol (E3) 10 pg/mL (LLOQ) Estrone (E1) 9000 4.28

8800
3.93 6000 8600
1.4e4 5800 4.82 8400
8200
1.4e4 5600
Equivalent reliability 8000
1.3e4 5400 7800
to traditional synthetic 7600
1.3e4 Novum SLE 5200 7400
1.2e4 5000 7200
7000
1.2e4 4800
6800 4.08
1.1e4 4600 6600
4400 6400
1.1e4 5.69
6200
1.0e4 3.08 4200 6000
4000 5800 3.92
9500 5600
9000 3800 5400 3.47

3600 5200
8500
5000
Intensity, cps

3400
8000 3.40 4800
3200 4600
Intensity, cps

Intensity, cps

7500
4400
3000
7000 4200
3.20 2800 4000
6500
2600 3800
6000 3600
2400 3400
5500
2.93 2200 3200
5000 3000 4.65
2000 2800
4500
2.36
2.52 1800 5.35 5.42 2600
4000 2400
2.63 1600 4.73
2200
3500
1400 2000
3000 1800 5.15
1200
1600
2500 1000 1400
2000 800 1200
1500
1000
600
App ID 26234

App ID 26232

App ID 26233

800
1000 400 600
400
500 200 200
0 0 0
2.2 2.4 2.6 2.8 3 3.2 3.4 3.6 3.8 4 min 4.8 5.0 5.2 5.4 5.6 5.8 6.0 6.2 6.4 min 3.4 3.6 3.8 4.0 4.2 4.4 4.6 4.8 5.0 min

For more applications and information about Novum PRO SLE, visit
www.phenomenex.com/NovumPRO

52 Phenomenex
Novum  Simplified Liquid Extraction (SLE)
™

Patent Pending

A Variety of Formats to Fit Your Sample

and Throughput Requirements

novum
simplified liquid extraction
Tubes

novum
simplified liquid extraction

novum
simplified liquid extraction
novum
simplified liquid extraction
Process samples as small as 200 μL

novum
simplified liquid extraction
novum
simplified liquid extraction

or as large as 2 mL using Novum novum

simplified liquid extraction
novum
simplified liquid extraction

SLE tubes. Ideal for all types of

applications including Bioanalytical,
Food Safety, and Environmental.

Ordering Information
Novum Simplified Liquid Extraction (SLE) Tubes
Novum SLE Tubes 1 cc 3 cc 6 cc 12 cc
Maximum Sample Volume
200 µL 400 µL 1 mL 2 mL
(after dilution)

SAMPLE PREPARATION
Recommended Elution Volume 2x 600 µL 2x 900 µL 2x 2.5 mL 2x 5mL

Part No. 8B-S138-FAK 8B-S138-5BJ 8B-S138-JCH 8B-S138-KDG

Unit 100/pk 50/pk 30/pk 20/pk

|
NOVUM AND NOVUM PRO SLE
96-Well Plates
Process 96 samples at once in an
easily automatable 96-well plate.
Perfect for high-throughput applica-
tions.

Ordering Information
Novum Simplified Liquid Extraction (SLE) 96-Well Plates
Novum SLE 96-Well Plates MINI MAX PRO MINI PRO MAX
Maximum Sample Volume
300 µL 400 µL 300 µL 400 µL
(after dilution)
Recommended Elution Volume 1x 1 mL 2x 900 µL 1x 1 mL 2x 900 µL
Part No. 8E-S138-FGA 8E-S138-5GA 8E-S539-FGA 8E-S539-5GA
Unit 1/pk 1/pk 1/pk 1/pk

For more information about Phenomenex

For accessories that are compatible with Novum Simplified Liquid
sample preparation products, visit
Extraction (SLE) Products, see pp. 79-82
www.phenomenex.com/sampleprepinfo

Phenomenex 53
 Strata DE Diatomaceous Earth SLE
™
Supported Liquid Extraction

A Cost Effective Supported

Liquid Extraction (SLE) Solution
Quickly and easily improve your liquid-liquid extractions by Recovery Values and % CVs: Strata DE vs. Biotage ISOLUTE SLE+
following a short, automatable two step extraction process. Biotage
Packed with Diatomaceous Earth, Strata DE is a great alternative Strata DE ISOLUTE SLE+
Analyte
to traditional SLE products such as Biotage® ISOLUTE® SLE+, % %CV % %CV
Recovery (n=8) Recovery (n=8)
Thermo HyperSep™ SLE, and Agilent® Chem Elut® SLE.
6-MAM 98 9 88 16
Alprazolam 104 10 98 11
Benzoylecgonine 88 6 98 11
SLE Protocol Buprenorphine 93 7 102 15
Pre-treatment: Combine 100 µL of spiked urine, 15 µL Campbell Beta-Glucuronidase Codeine 99 12 93 9
(part number: DR2102), 35 µL 100 mM Ammonium Acetate (pH 4), and Diazepam 107 7 104 6
150 µL of 100 mM Ammonium Bicarbonate (pH 10).
Fentanyl 85 5 94 8
96-Well Plates: Strata DE 400 µL
Biotage ISOLUTE SLE+ 400 µL Hydrocodone 104 11 93 11
Hydromorphone 95 9 93 11
Part No.: 8E-S325-5GB (Strata DE)
Lorazepam 94 8 98 8
Load: 300 µL pre-treated urine sample onto plate (apply vacuum or positive
pressure to pull/push sample into sorbent if necessary) Methamphetamine 92 16 102 8
Morphine 98 12 94 12
Wait: 6 minutes
Norbuprenorphine 101 11 92 11
Elute: 3x 600 µL Dichloromethane/IPA (95:5)
Nordiazepam 100 9 92 8
Apply: Vacuum or apply positive pressure at 5-10” Hg for 10 seconds
Norfentanyl 113 7 110 11
Dry: Sample under slow stream of Nitrogen at 30 °C Oxycodone 97 5 93 11
STRATA DE DIATOMACEOUS EARTH SLE | SAMPLE PREPARATION

Reconstitute: 100 µL 0.1% Formic Acid/Methanol (4:1) with internal standard PCP 90 7 98 6

A Fast Extraction of 25-OH

Vitamin D2 / D3 from Serum
Strata DE provides a simple extraction method with time and cost
savings across all 3 QC levels.

SLE Protocol Accuracy and Precision

Pre-treatment: Dilute 200 µL of human serum* with 100 µL of 5 % Ammonium hydroxide QCL QCM QCH
(w/v), add 25 µL of 25-OH Vitamin-D3- 2H6 (1 µg/mL) and mix.
Target Conc. (ng/mL) 6 50 80
96-Well Plate: Strata DE 400 µL 25-OH-D2
Part No.: 8E-S325-5GB Mean Conc. Found 5.92 53.0 80.8
Load: Pre-treated sample and wait for 5 minutes STDV 4.09 2.21 5.55
Elute: Sample with 600 µL MTBE by gravity, wait for 5 minutes CV% 6.90 4.18 6.86
Repeat: Elution step twice by gravity, and after the final elution, apply 5-10 Hg Accuracy (%) 98.7 106 101
vacuum to finish elution n 6 6 6
Dry: 40 °C under N2 25-OH-D3

Reconstitute: 200 µL 0.1 % Formic acid in Water/0.1 % Formic acid in Methanol (30:70) Mean Conc. Found (ng/mL) 6.59 52.7 87.2
STDV 0.50 1.74 5.50
CV% 7.62 3.30 6.31
Accuracy (%) 110 105 109
n 6 6 6

* Double Charcoal-stripped human serum was used to prepare all standards and QCs

Comparative separation may not be representative of all applications.

54 Phenomenex
Strata DE Diatomaceous Earth SLE
™

Available for Large Volume Samples

and High-throughput Cleanups

Tubes
Ideal for large volume cleanups
such as Food and Environmental
applications.

Ordering Information
Strata DE (Diatomaceous Earth SLE Tubes)
Strata DE Tube 2 mL Capacity, 12 cc 20 mL Capacity, 60 cc
Maximum Sample Volume
2 mL 17 mL
(after dilution)
Recommended Elution Volume 2x 5 mL 3x 20 mL

Part No. 8B-S325-KDG 8B-S325-VFF

Unit 20/pk 16/pk

SAMPLE PREPARATION
96-Well Plates

|
Ideal for smaller volume,

STRATA DE DIATOMACEOUS EARTH SLE

high-throughput cleanups such as
Bioanalytical samples.

Ordering Information
Strata DE (Diatomaceous Earth SLE) 96-Well Plates
Strata DE 96-Well Plates 200 µL 400 µL
Maximum Sample Volume
200 µL 300 µL
(after dilution)
Recommended Elution Volume 2x 600 µL 3x 600 µL
Part No. 8E-S325-FGB 8E-S325-5GB
Unit 2/pk 2/pk

For accessories that are compatible with Strata DE Supported For more information on Strata DE, visit
Liquid Extraction (SLE) Products, see pp. 79-82 www.phenomenex.com/stratade

Recommended volumes are the expected loadability for most samples, however, it may be possible to
load more than the stated capacity without breakthrough of the sample.

Comparative separations may not be representative of all applications.

Phenomenex 55
 β-Gone ™
β-Glucuronidase Removal Products β-Glucuronidase Removal Products

Rapid Cleanup of Hydrolyzed Urine

β-Gone β-Glucuronidase Removal Products are designed to target and remove β-glucuronidase from
hydrolyzed urine samples without requiring additional time or method development. In a single step
and in less than 1 minute, your hydrolyzed samples are ready for analysis.

• Increase HPLC/UHPLC column lifetime

• Reduce mass spec maintenance
• Maintain the selectivity of your HPLC/UHPLC column
• Perform the pre-treatment step inside the 96-well plate with β-Gone Plus

β-Gone Plus Steps Now In-Well Hydrolysis

Capabilities!

2 under
Load Collect 1
minute
Load urine and hydrolysis Initiate vacuum and
solution in β-Gone Plus Skip the Transfer Step collect eluate
96-well plate, incubate
| SAMPLE PREPARATION

Increase Your Sensitivity:

β-Gone vs. Dilute-and-Shoot

3.6e4
3.4e4 >3x more sensitive
3.2e4
3.0e4
than dilute-and-shoot!
2.8e4
2.6e4 THC-COOH
2.4e4
2.2e4
2.0e4
1.8e4
β-GONE BETA-GLUCURONIDASE REMOVAL PRODUCTS

Intensity, cps

1.6e4
1.4e4
1.2e4 β-Gone
App ID 23627

10e4
8000
6000 Dilute-and-Shoot
4000
2000
0
0 1 2 3 4 5 6 min
β-Gone Procedure: To 200 µL spiked urine (spiked at 100 ng/mL), add 133 µL 0.1 % Formic acid in Methanol. Pass through β-Gone tube or 96-well
plate and collect eluent. Dilute-and-Shoot Procedure: Dilute spiked urine (spiked at 100 ng/mL) 10-fold with 0.1 % Formic acid in Water.

Column: Kinetex® 2.6 µm Biphenyl

Dimensions: 50 x 2.1 mm
Part No.: 00B-4622-AN
Mobile Phase: A: 0.1 % Formic acid in Water
B: 0.1 % Formic acid in Acetonitrile
Gradient: Time (min) % B
0 5
3 95
4 95
4.1 5
Flow Rate: 500 µL/min
Temperature: Ambient
Detection: MS/MS (SCIEX® API 4000™)

Ordering Information
β-Gone β-Glucuronidase Removal Products
Part No. Description Unit
8B-S139-TAK 1 mL Tubes, Recombinant Enzyme 100/Box Watch the Webinar
8B-S322-DAK 1 mL Tubes, Non-Recombinant Enzyme 100/Box Learn how to instantly improve your sensitivity without
introducing extra steps into your workflow!
8E-S323-TGA 96-Well Plate Plus 30 mg/well, Recombinant/Non-Recombinant Enzyme 1/Box
8E-S323-UGA 96-Well Plate Plus 60 mg/well, Recombinant/Non-Recombinant Enzyme 1/Box
www.phenomenex.com/BetaGone
8N-S323-TUK 2 mL Centrifuge Tubes, Recombinant and Non-Recombinant Enzyme 100/Box

56 Phenomenex
Impact ™
Protein Precipitation Plates

Rapid Protein Precipitation General Protocol

• Quickly cleanup sample by passing biological samples
through the Impact filter Dispense
Organic solvent into the wells of the Impact plate
• Increase sensitivity of your analysis by eliminating in a volume of 3 - 4x the volume of the intended
proteins which contribute to baseline noise plasma or tissue homogenate sample. Recom-
• Increase reproducibility with the leak-free membrane, mended solvents and maximum volume of sample
preventing premature sample breakthrough and and precipitation solvent are listed on this page.
incomplete protein precipitation

Solvent Add*
Shielding Plasma or tissue homogenate directly and forcefully
Technology™
prevents into the organic solvent, maintain a final ratio of
leaks 3:1 to 4:1 organic solvent:sample. Recommended
sample volumes are listed on this page.

Leak Leak-free
occurs
Standard
Impact Product Vortex†
Precipitation Product
2 minutes at maximum possible speed, taking care
not to allow solvent spillage. Sample can stand for
Can retain acetonitrile with no leaks for up to 25 minutes.
up to 25 minutes

SAMPLE PREPARATION
Compatible Solvents Solvent : Sample Ratio
Acetonitrile 3:1 to 4:1 Filter
Methanol 4:1 Centrifuge:
Maximum Total Combined Liquid Volume (Organic Solvent plus Biological Sample) Place the Impact plate on top of a collection plate
96-well plates 1.6 mL
and centrifuge at 500 g for 5 minutes or until filtrate
Recommended Biological Sample Volumes
is collected.
96-well plates 25-400 µL

| IMPACT PROTEIN PRECIPITATION PLATES

Leak Resistant Time Vacuum:
96-well plates Up to 25 minutes with no vacuum/pressure Place the Impact plate onto a suitable 96-well
sample manifold or robot. Ensure that a 96-well
Ordering Information collection plate is positioned inside the manifold or
Impact Precipitation Products under the Impact plate. Vacuum at 2 - 7 inch Hg for
Part No. Description Unit up to 5 minutes or until filtrate is collected.
Impact Precipitation Products Positive Pressure:
CE0-7565 Impact Protein Precipitation, Square Well, 2/pk
Filter Plate, 2 mL Place the Impact plate on top of a collection
CE0-7566 Impact Protein Precipitation, Square Well, 2/pk plate and apply 2 - 5 psi using a positive pressure
Long Drip, Filter Plate, 2 mL manifold.
CE0-9343 Impact Protein Precipitation, 2mL Square Well Filter 25/pk
Plate
CE0-9344 Impact Protein Precipitation, Long Drip 2mL Square Well 25/pk
Filter Plate
* A 3:1 v/v ratio of organic solvent to biological sample will dilute your
Impact Starter Kit for Protein Precipitation
sample less. In contrast, a 4:1 v/v ratio of organic solvent to biological
CE0-8201 Impact Protein Precipitation Plate (CE0-7565) (2 ea) ea sample will ensure a more complete precipitation. A 4:1 v/v ratio is
Collection Plate 2 mL (2 ea) recommended when using methanol.
Sealing Mat, Santoprene™ (AH0-8199) (2 ea)
†
When used with a liquid-handling instrument or automation, aspirate/
dispense cycles may be used to promote in-tip mixing and precipitation.
This will ensure complete precipitation and filtration. Vortexing is not
necessary when in-tip precipitation is performed.

For Accessories, see pp. 79-82

Phenomenex 57
 Phree ™
Phospholipid Removal Solutions Phospholipid Removal Solutions

2013 R&D 100

Eliminate Ion Suppression with Phree Award Recipient

• Consistently remove > 99 % of phospholipids to increase LC-MS sensitivity

• Simultaneously remove interfering proteins
• No additional time required, the Phree method can be performed in
the same amount of time as a protein precipitation procedure
• Skip the method development; one method for acids, bases, and neutrals

How it Works:
2
Eliminate Phospholipids
The Phree sorbent selectively
Remove Proteins removes phospholipids from
Solvent Shielding Technology™ prevents precipitated plasma samples.
dripping of organic solvent, allowing
for protein precipitation within the wells
of the Phree Phospholipid Removal
Product.

3
No Method Development
| SAMPLE PREPARATION

One method for acids,

bases, and neutrals.

Proteins
Total Phospholipid Profile Phospholipids
Protein Precipitation vs. Phree Phospholipid Removal Products Target Analyte
20011

4.5e6
PHREE PHOSPHOLIPID REMOVAL SOLUTIONS

4.0e6

3.5e6
Protein precipitation does not
remove phospholipids
3.0e6
Intensity, cps

2.5e6

2.0e6
App ID 20011

1.5e6

1.0e6

5.0e5

0
0 1 2 3 4 5 6 7 8 9 10 11 min

Plasma Cleanup: 100 µL plasma plus 300 µL Acetonitrile

with 1 % Formic acid
~ 50x Zoom Column: Kinetex® 2.6 µm C18 100 Å
Dimensions: 50 x 2.1 mm
20012
Part No.: 00B-4462-AN
Mobile Phase: A: 0.1 % Formic acid in Water
B: 0.1 % Formic acid in Methanol
9.0e4
Gradient: Time (min) % B
8.5e4
8.0e4
0 60
7.5e4
0.5 95
7.0e4 15.5 95
6.5e4 15.51 60
6.0e4 19.5 60
Intensity, cps

5.5e4
Flow Rate: 0.4 mL/min
5.0e4 Selectively remove phospholipids Detection: Mass Spectrometer (MS) @ 425 °C;
4.5e4
with Phree
App ID 20012

4.0e4 184 amu
3.5e4 Temperature: 22 °C
3.0e4
2.5e4
2.0e4
Phree extracted plasma
1.5e4
1.0e4 Protein precipitated plasma
5000.0
0.0
0 1 2 3 4 5 6 7 8 9 10 11 min

Phospholipid profile monitored using m/z 184-184

58 Phenomenex
Phree ™
Phospholipid Removal Solutions

Reduce Ion Suppression

The presence of phospholipids in plasma samples produces zones
of ion suppression that correlate exactly with the phospholipid
elution profile when analyzed via mass spectrometer (MS).

Protein Precipitated Plasma Phree Extracted Plasma

20013
20014

1.2e6 1.2e6
Ion suppression
1.0e6
is observed
1.0e6

Suppression zone
8.0e5 8.0e5 is eliminated

Intensity, cps
Intensity, cps

6.0e5 6.0e5

4.0e5 4.0e5
App ID 20013

App ID 20014
2.0e5 2.0e5

0
0.0 0 0.2 0.6 1.0 1.4 1.8 2.2 2.6 min
0.2 0.6 1.0 1.4 1.8 2.2 2.6 min

Suppression Zone Amoxapine was infused post-column to establish an ion suppression/enhancement profile with both protein precipitated
plasma (left) and Phree extracted plasma (right), showing that Phree can successfully reduce ion suppression.

SAMPLE PREPARATION
Phospholipids m/z 184-184
Amoxapine m/z 314-271

Maximize Sensitivity and Column Lifetime

Phospholipids reduce the sensitivity of the MS signal and shorten column lifetime when they build up over time.

Column Sensitivity after 250 Injections

| PHREE PHOSPHOLIPID REMOVAL SOLUTIONS

300,000

~5,000 µL plasma injected without

significant loss of sensitivity
250,000

200,000

Diclofenac in Phree
Peak Area Counts

Reduced sensitivity is
immediately observed
Extracted Plasma
150,000

Diclofenac in Protein
Precipitated Plasma
100,000

Phospholipid build up results in

significant loss of sensitivity
50,000

0
0 50 100 150 200 250
Injection Number (20 µL of Plasma)

To assess the effect of phospholipid build up, repetitive 20 µL injections of diclofenac in protein precipitated plasma versus diclofenac in
Phree extracted plasma were made.

Ordering Information
Phree Phospholipid Removal Products For accessories that are compatible
with Phree Phospholipid Removal
Part No. Description Unit
Products, see pp. 79-82
8B-S133-TAK Phree Phospholipid Removal Tabbed 1 mL Tubes 100/pk
8E-S133-TGB Phree Phospholipid Removal 96-Well Plates 2/pk

Phenomenex 59
 Strata -X PRO Polymeric SPE ™

Polymeric Sorbent with RAPID ER

EXPR

SUP

ES
Matrix Removal Technology

S
Strata-X PRO works to eliminate phospholipids and harmful par-
ticulates in the sample while targeting analytes. Strata-X PRO pro-
vides high recoveries, especially for polar analytes, and less matrix NO CONDITION OR EQUILIBRATION STEPS!
effects that could result in ion suppression or enhancement when
compared to traditional polymeric SPE.
1 LOAD SAMPLE 1 LOAD SAMPLE
• Reversed phase polymeric sorbent designed to be Analytes Analytes
Impurities Impurities
water wettable
• Reduce protocol time by at least 40% with 3-Step and
2-Step SPE
• High recoveries without conditioning or equilibration
2 WASH IMPURITIES
Wash Solvent

SPE Protocol
Impurities are
96-Well Plate: Strata-X PRO, 30 mg/well washed off
Part No.: 8E-S536-TGA the sorbent
Load: 400 µL Plasma/0.1 % Formic acid in
Water (1:1) 3 ELUTE ANALYTES 2 ELUTE ANALYTES
Wash: 1 mL 5 % Methanol in Water Elution Elution
Dry: 1 minute at 5" Hg Solvent Solvent
Elute: 1 mL 0.1 % Formic acid in Acetonitrile/
Methanol (90:10)
Dry Down: 1 minute at 5" Hg
STRATA-X PRO POLYMERIC SPE | SAMPLE PREPARATION

Reconstitute: 200 µL 5 % Methanol in Water Analytes Analytes

elute elute

Consistently high recoveries, with less variation

between samples and less matrix effects using Strata-X PRO SPE
Strata-X PRO.
Traditional Polymeric SPE
Recovery from Human Plasma Matrix Effects
120 40%
110
100 20%
90
0%
80
Recovery (%)

70 -20%
Matrix Effect (%)

60
50 -40%
40
-60%
30
20 -80%
10
0 -100%
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Ordering Information
Format Sorbent Mass Part Number Unit Format Sorbent Mass Part Number Unit
Tube 96-Well Plate
10 mg 8B-S536-AAK 1 mL (100/box) 10 mg/well 8E-S536-AGA ea
30 mg 8B-S536-TAK 1 mL (100/box) 30 mg/well 8E-S536-TGA ea
30 mg 8B-S536-TBJ 3 mL (50/box) 60 mg/well 8E-S536-UGA ea
60 mg 8B-S536-UBJ 3 mL (50/box) 96-Well Microelution Plate
200 mg 8B-S536-FBJ 3 mL (50/box) 2 mg/well 8M-S536-4GA ea
100 mg 8B-S536-ECH 6 mL (30/box)
200 mg 8B-S536-FCH 6 mL (30/box)
500 mg 8B-S536-HCH 6 mL (30/box)
Giga™ Tube
1 g/20 mL 8B-S536-JEG 20/pk

60 Phenomenex
Strata -X Polymeric SPE
™
Polymeric SPE

U.S. Patent No. 7,119,145

Strata-X
Step 1. Select a Sorbent
Compound-Directed Phase Selection

Strong Acids Strata-X-AW

(pKa < 2)
Acidic Compounds Strata-XL-AW

Strata-X-A
Weak Acids
(pKa 2–4)
Strata-XL-A

Strata-X
Neutral
Neutral Compounds Compounds
Strata-XL

Strata-X-C
Weak Bases
(pKa 8–10)
Strata-XL-C

Basic Compounds Strata-X-CW

Strong Bases
(pKa > 10) Strata-XL-CW

SAMPLE PREPARATION
Specialty Sorbents Application Phase Description

Proprietary strong cation-exchange sorbent that eliminates the need to

Strata-X-Drug B Basic Drugs of Abuse condition / equilibrate the sorbent.
*Now available with in-well hydrolysis capabilities

|
Proprietary reversed phase sorbent that eliminates the need to
Strata-X-Drug N Neutral Drugs of Abuse
condition / equilibrate the sorbent.

STRATA-X POLYMERIC SPE

Step 2. Select a Sorbent Mass
Loading Capacity Chart
Plasma Filtered Tissue Water Water Mass
Strata-X Phase /Serum Urine Homogenates (particulate-free) (particulate-laden) (mg in tube)

100 µL 250 µL 10 mg N.A. N.A. 10 mg

250 µL 1 mL 50 mg N.R. N.R. 30 mg
500 µL 2 mL 100 mg N.R. N.R. 60 mg
Strata-X, X-C, X-CW, X-A, X-AW
1 mL 4 mL 150 mg 50 mL 25 mL 100 mg
N.A. 8 mL 300 mg 100 mL 50 mL 200 mg
N.A. 20 mL 500 mg 500 mL 100 mL 500 mg
50 µL 125 µL 5 mg N.A. N.A. 10 mg

125 µL 500 µL 25 mg N.R. N.R. 30 mg

250 µL 1 mL 50 mg N.R. N.R. 60 mg
Strata-XL, XL-C, XL-CW, XL-A, XL-AW
500 µL 2 mL 75 mg 25 mL 13 mL 100 mg
N.A. 4 mL 150 mg 50 mL 25 mL 200 mg
N.A. 10 mL 250 mg 250 mL 50 mL 500 mg

N.A. = Not Applicable (not commonly used)

N.R. = Not Recommended (may not provide expected results) See the following pages for specific phase details
and general extraction protocols.

Phenomenex 61
 Strata -X Polymeric SPE
™

General Extraction Protocols

Strata-X-C / Strata-XL-C Strata-X-CW / Strata-XL-CW

Strong Cation-Exchange & Reversed Phase Weak Cation-Exchange & Reversed Phase

for Bases with pKa ≤ 10.5 for Bases with pKa > 8

Condition Condition
1 mL Methanol 1 mL Methanol
Equilibrate Equilibrate
Bases

1 mL Acidified Water 1 mL Water, pH 6-7

Load Load
Diluted Acidified Sample Diluted Sample, pH 6-7
Wash Wash
1 mL 0.1 N HCI in water (collect this fraction to analyze Polar Neutrals) 1 mL Water, pH 6-7
Wash Wash
1 mL 0.1 N HCI in Methanol (collect this fraction to analyze Neutrals/Acids) 1 mL Methanol (collect this fraction to analyze Neutrals/Acids)

Elute Bases Elute Any Base Elute Weak Bases

2x 500 µL 5 % NH4OH in Methanol 2x 500 µL 5 % Formic Acid in Methanol 2x 500 µL 5 % NH4OH in Methanol

Strata-X / Strata-XL
Reversed Phase
STRATA-X POLYMERIC SPE | SAMPLE PREPARATION

for Neutral Compounds

Condition
Neutrals

1 mL Methanol
Equilibrate
1 mL Water
Load
Diluted Sample
Wash
1 mL 5-60 % Methanol
Elute
2x 500 µL 2 % Formic Acid in Methanol/Acetonitrile

Strata-X-A / Strata-XL-A Strata-X-AW / Strata-XL-AW

Strong Anion-Exchange & Reversed Phase Weak Anion-Exchange & Reversed Phase
for Acids with pKa > 2 for Acids with pKa ≤ 5

Condition Condition
1 mL Methanol 1 mL Methanol
Equilibrate Equilibrate
Acids

1 mL Water, pH 6-7 1 mL Water, pH 6-7

Load Load
Diluted Sample, pH 6-7 Diluted Sample, pH 6-7
Wash Wash
1 mL 25 mM Ammonium Acetate Buffered, pH 6-7 1 mL 25 mM Ammonium Acetate Buffered, pH 6-7
Wash Wash
1 mL Methanol (collect this fraction to analyze Neutral/Bases) 1 mL Methanol

Elute Acids Elute Any Acid

Elute Weak Acids
2x 500 µL 5 % Formic Acid in Methanol 2x 500 µL 5 % NH4OH in Methanol 2x 500 µL 5 % Formic Acid in Methanol

*Based on 30 mg/1 mL sorbent mass.

The above is a convenient starting point for SPE method development.
Further optimization may be required to tailor the method to your specific needs.

62 Phenomenex
Strata -X Polymeric SPE
™

U.S. Patent No. 7,119,145

Microelution SPE A Faster SPE Solution
Successful bioanalytical sample Save 30 or more minutes per 96-well plate by eliminating
lengthy dry down and reconstitution steps.
preparation without the dry down
• Save 30 or more minutes per 96-well plate! 200 µL
Step
• At least 8x more sensitive than traditional 10 mg SPE Condition
• Elution volumes as low as 25 µL 1

Step 200 µL
Equilibrate
2
sample
volumes
Step as low as
10 µL Load Sample
3
For ordering information, go to pages 64-68

Step 200-400 µL

SAMPLE PREPARATION
Wash
4
Strata-X Microelution Method
Development and Peptide Screening Step Elute
Test different SPE chemistries using a single 96-well plate
5
At least 8x more sensitive than
traditional 10 mg SPE!
25 µL

|
STRATA-X POLYMERIC SPE
Ordering Information Dry Down
Strata-X Microelution Peptide Screening 96-Well Plates Step and Reconstitute
NOT
Part No. Description Unit Save 30 or more minutes per
KS0-9528 Strata-X-CW 2 mg/well (6 rows)
Strata-X-A 2 mg/well (6 rows)
ea 6 REQUIRED
96-well plate! Save on labor costs,
do more in less time.
Strata-X Microelution Method Development 96-Well Plates
Part No. Description Unit
KS0-9529 Strata-X-C 2 mg/well (3 rows) ea Total Processing Time
Strata-X-AW 2 mg/well (3 rows)
Strata-X-CW 2 mg/well (3 rows)
Strata-X-A 2 mg/well (3 rows)
30 Minutes
No Dry Down!

Throughput (in 8 hours)

DOUBLE
your throughput!*

REDUCE
Cost per Sample!*

* When compared to traditional SPE methods

Download starting methods at:

www.phenomenex.com/microelution

Phenomenex 63
 Strata -X Polymeric SPE ™

U.S. Patent No. 7,119,145

Strata-X and Strata-XL
A reversed phase functionalized polymeric sorbent that gives strong retention of neutral,
acidic, or basic compounds under aggressive, high organic wash conditions.

Strata-X, 33 µm, 85 Å Strata-XL, 100 µm, 300 Å

High Concentration Samples X
Small Target Analytes (< 10 kDa) X
Large Target Analytes (> 10 kDa) X
Large Volume Samples X
Viscous Samples X

3 Mechanisms of Retention
π-π Bonding Hydrogen Bonding Dipole-Dipole Interactions Hydrophobic Interaction

n O n O n O
)
)

)
.. .. ..
N N N
)
)

)
Strata-X Strata-XL
Ordering Information Ordering Information
Format Sorbent Mass Part Number Unit Format Sorbent Mass Part Number Unit
STRATA-X POLYMERIC SPE | SAMPLE PREPARATION

Tube Tube
30 mg 8B-S100-TAK** 1 mL (100/box) 30 mg 8B-S043-TAK 1 mL (100/box)
30 mg 8B-S100-TBJ 3 mL (50/box) 60 mg 8B-S043-UBJ 3 mL (50/box)
60 mg 8B-S100-UBJ** 3 mL (50/box) 100 mg 8B-S043-EBJ 3 mL (50/box)
100 mg 8B-S100-EBJ 3 mL (50/box) 200 mg 8B-S043-FBJ 3 mL (50/box)
100 mg 8B-S100-ECH 6 mL (30/box) 200 mg 8B-S043-FCH 6 mL (30/box)
200 mg 8B-S100-FBJ 3 mL (50/box) 500 mg 8B-S043-HCH 6 mL (30/box)
200 mg 8B-S100-FCH 6 mL (30/box) Giga Tube
500 mg 8B-S100-HBJ 3 mL (50/box) 2g 8B-S043-KDG 12 mL (20/box)
500 mg 8B-S100-HCH 6 mL (30/box) 2g 8B-S043-KEG 20 mL (20/box)
Giga™ Tube 5g 8B-S043-LEG 20 mL (20/box)
500 mg 8B-S100-HDG 12 mL (20/box) 5g 8B-S043-LFF 60 mL (16/box)
1g 8B-S100-JDG 12 mL (20/box) 10 g 8B-S043-MFF 60 mL (16/box)
1g 8B-S100-JEG 20 mL (20/box) 96-Well Plate
2g 8B-S100-KEG 20 mL (20/box) 30 mg 8E-S043-TGB 2 Plates/Box
5g 8B-S100-LFF 60 mL (16/box)
Teflon® Tube
200 mg 8B-S100-FBJ-T 3 mL (50/box)
200 mg 8B-S100-FDG-T 12 mL (20/box) * To control flow rate with Strata-XL, use a stopcock (AH0-6048)
when processing samples with a vacuum manifold.
96-Well Plate
10 mg 8E-S100-AGB 2 Plates/Box
30 mg 8E-S100-TGB 2 Plates/Box
60 mg 8E-S100-UGB 2 Plates/Box
96-Well Microelution Plate
2 mg 8M-S100-4GA ea

On-line Extraction Cartridge

Description Part Number Unit/Box
Strata-X on-line extraction 00M-S033-B0-CB ea
cartridge, 20 x 2.0 mm
Cartridge holder, 20 mm CH0-5845 ea

**Tab-less tubes available. Contact Phenomenex for details.

For Large Volume Cleanup use Giga Tubes Create a customized SPE method in under 1 minute.
For SPE Vacuum Manifolds and Accessories, see pp. 79-82 www.phenomenex.com/mdtool

64 Phenomenex
Strata -X Polymeric SPE ™

U.S. Patent No. 7,119,145

Strata-X-C and Strata-XL-C O
A strong cation-exchange functionalized polymeric sorbent that +

=
S – O –-H

)
allows for complete retention of basic compounds with a pKa less
than 10.5, making 100 % organic wash conditions possible.

=
O

)
Strata-X-C, 33 µm, 85 Å Strata-XL-C, 100 µm, 300 Å
High Concentration Samples X
Small Target Analytes (< 10 kDa) X
Large Target Analytes (> 10 kDa)
O O
+X

=
S – O –-H XX

)
+

=
S–O–H
Large Volume Samples

)
-
Viscous Samples

=
O O
)

)
3 Mechanisms of Retention
Strong Cation-Exchange π-π Bonding Hydrophobic Interaction

O O O
+ +

=
=
S–O H S – O –-H
+

)
=

S – O –-H
-
)

=
=
=

O O O

)
)
)

Strata-X-C Strata-XL-C
O
Ordering Information O Ordering Information
+
=

-H
)

+
S–O –
=

–H
Format Sorbent Mass Part
- Number Unit
)

Format Sorbent Mass Part Number Unit

S – O

SAMPLE PREPARATION
Tube
=

Tube
O
=

30 mg 8B-S029-TAK** 1 mL (100/box) 30 mg 8B-S044-TAK 1 mL (100/box)

30 mgO
)

8B-S029-TBJ 3 mL (50/box) 60 mg 8B-S044-UBJ 3 mL (50/box)

)

60 mg 8B-S029-UBJ** 3 mL (50/box) 100 mg 8B-S044-EBJ 3 mL (50/box)

100 mg 8B-S029-EBJ 3 mL (50/box) 100 mg 8B-S044-ECH 6 mL (30/box)
100 mg 8B-S029-ECH 6 mL (30/box) 200 mg 8B-S044-FBJ 3 mL (50/box)
O
200 mg 8B-S029-FBJ 3 mL (50/box) 200 mg 8B-S044-FCH** 6 mL (30/box)
+
=

–-H
)

200 mg 8B-S029-FCH 6 mL (30/box) 500 mg 8B-S044-HBJ 3 mL (50/box)

S
500 mg
– O8B-S029-HBJ 3 mL (50/box) 500 mg 8B-S044-HCH 6 mL (30/box)

|
=

500 mg 8B-S029-HCH 6 mL (30/box) Giga Tube

O

STRATA-X POLYMERIC SPE

)

Giga™ Tube 2g 8B-S044-KDG 12 mL (20/box)

500 mg 8B-S029-HDG 12 mL (20/box) 2g 8B-S044-KEG 20 mL (20/box)
1g 8B-S029-JDG 12 mL (20/box) 5g 8B-S044-LEG 20 mL (20/box)
1g 8B-S029-JEG 20 mL (20/box) 5g 8B-S044-LFF 60 mL (16/box)
2g 8B-S029-KEG 20 mL (20/box) 10 g 8B-S044-MFF 60 mL (16/box)
5g 8B-S029-LFF 60 mL (16/box) 96-Well Plate
96-Well Plate 30 mg 8E-S044-TGB 2 Plates/Box
10 mg 8E-S029-AGB 2 Plates/Box
30 mg 8E-S029-TGB 2 Plates/Box
60 mg 8E-S029-UGB 2 Plates/Box
96-Well Microelution Plate
2 mg 8M-S029-4GA ea

On-line Extraction Cartridge

Description Part Number Unit/Box
Strata-X-C on-line extraction 00M-S048-B0-CB ea
cartridge, 20 x 2.0 mm
Cartridge holder, 20 mm CH0-5845 ea

**Tab-less tubes available. Contact Phenomenex for details.

Create a customized SPE method in under 1 minute.

For Large Volume Cleanup use Giga Tubes
For SPE Vacuum Manifolds and Accessories, see pp. 79-82 www.phenomenex.com/mdtool

Phenomenex 65
 Strata -X Polymeric SPE ™

U.S. Patent No. 7,119,145

Strata-X-CW and Strata-XL-CW
A weak cation-exchange functionalized polymeric sorbent that allows for complete
retention of basic compounds with a pKa greater than 8, including quaternary amines,
making 100 % organic wash conditions possible.

)
O
Strata-X-CW, 33 µm, 85 Å Strata-XL-CW, 100 µm, 300 Å

)
High Concentration Samples X
OH
Small Target Analytes (< 10 kDa) X
Large Target Analytes (> 10 kDa) X
Large Volume Samples X

)
Viscous Samples X

)
O O
3 Mechanisms of Retention )

)
Weak Cation-Exchange π-π Bonding OH Hydrophobic Interaction OH
)

)
O O O
)

OH

)
OH OH
Strata-X-CW Strata-XL-CW
)

Ordering Information Ordering Information

)

Format Sorbent Mass O

Part Number Unit Format O Sorbent Mass Part Number Unit
STRATA-X POLYMERIC SPE | SAMPLE PREPARATION

Tube Tube
)

30 mg 8B-S035-TAK** 1 mL (100/box) 30 mg 8B-S052-TAK 1 mL (100/box)

)

30 mg
OH
8B-S035-TBJ 3 mL (50/box) OH 60 mg 8B-S052-UBJ 3 mL (50/box)
60 mg 8B-S035-UBJ** 3 mL (50/box) 100 mg 8B-S052-EBJ 3 mL (50/box)
100 mg 8B-S035-ECH 6 mL (30/box) 100 mg 8B-S052-ECH 6 mL (30/box)
200 mg 8B-S035-FBJ 3 mL (50/box) 200 mg 8B-S052-FBJ 3 mL (50/box)
)

200 mg O
8B-S035-FCH 6 mL (30/box) 200 mg 8B-S052-FCH 6 mL (30/box)
500 mg 8B-S035-HBJ 3 mL (50/box) 500 mg 8B-S052-HBJ 3 mL (50/box)
500 mg 8B-S035-HCH 6 mL (30/box) 500 mg 8B-S052-HCH 6 mL (30/box)
)

Giga™ Tube OH Giga Tube

1g 8B-S035-JDG 12 mL (20/box) 2g 8B-S052-KEG 20 mL (20/box)
1g 8B-S035-JEG 20 mL (20/box)
2g 8B-S035-KEG 20 mL (20/box) 96-Well Plate
5g 8B-S035-LFF 60 mL (16/box) 30 mg 8E-S052-TGB 2 Plates/Box
96-Well Plate
10 mg 8E-S035-AGB 2 Plates/Box
30 mg 8E-S035-TGB 2 Plates/Box
60 mg 8E-S035-UGB 2 Plates/Box
96-Well Microelution Plate
2 mg 8M-S035-4GA ea

On-line Extraction Cartridge

Description Part Number Unit/Box
Strata-X-CW on-line extraction 00M-S036-B0-CB ea
cartridge, 20 x 2.0 mm
Cartridge holder, 20 mm CH0-5845 ea

**Tab-less tubes available. Contact Phenomenex for details.

Create a customized SPE method in under 1 minute.

For Large Volume Cleanup use Giga Tubes
For SPE Vacuum Manifolds and Accessories, see pp. 79-82 www.phenomenex.com/mdtool

66 Phenomenex
Strata -X Polymeric SPE ™

U.S. Patent No. 7,119,145

Strata-X-A and Strata-XL-A
A strong anion-exchange functionalized polymeric sorbent that allows for complete
retention of weakly acidic compounds with pKa greater than 2, making 100 % organic

)
wash conditions possible. CH3
N+

)
Strata-X-A, 33 µm, 85 Å Strata-XL-A, 100 µm, 300 Å
High Concentration Samples X
CH3
Small Target Analytes (< 10 kDa) X
Large Target Analytes (> 10 kDa) X

)
Large Volume Samples X
CH3

)
Viscous Samples X
CH3
N+
N+
)

)
3 Mechanisms of Retention CH3
Strong Anion-Exchange π-π Bonding CH3
Hydrophobic Interaction
)

)
CH3 CH3 CH3
+
N N+ N+
)

)
CH3 CH3 CH3

Strata-X-A Strata-XL-A
)

Ordering Information Ordering Information

)

Format CH3 Part Number

Sorbent Mass Unit CH3
Format Sorbent Mass Part Number Unit

SAMPLE PREPARATION
Tube
N + N+
Tube
)
)

30 mg 8B-S123-TAK** 1 mL (100/box) 30 mg 8B-S053-TAK 1 mL (100/box)

CH
30 mg3 8B-S123-TBJ 3 mL (50/box) CH3 60 mg 8B-S053-UBJ 3 mL (50/box)
60 mg 8B-S123-UBJ 3 mL (50/box) 100 mg 8B-S053-EBJ 3 mL (50/box)
100 mg 8B-S123-EBJ 3 mL (50/box) 100 mg 8B-S053-ECH 6 mL (30/box)
100 mg 8B-S123-ECH 6 mL (30/box) 200 mg 8B-S053-FBJ 3 mL (50/box)
)

200 mg 8B-S123-FBJ 3 mL (50/box) 200 mg 8B-S053-FCH 6 mL (30/box)

CH3
200 mg 8B-S123-FCH 6 mL (30/box) 500 mg 8B-S053-HCH 6 mL (30/box)
500 mgN+ 8B-S123-HBJ 3 mL (50/box) Giga Tube

|
)

500 mg 8B-S123-HCH 6 mL (30/box) 2g 8B-S053-KEG 20 mL (20/box)

CH3

STRATA-X POLYMERIC SPE

Giga™ Tube 5g 8B-S053-LFF 60 mL (16/box)
500 mg 8B-S123-HDG 12 mL (20/box) 10 g 8B-S053-MFF 60 mL (16/box)
1g 8B-S123-JDG 12 mL (20/box) 96-Well Plate
1g 8B-S123-JEG 20 mL (20/box) 30 mg 8E-S053-TGB 2 Plates/Box
2g 8B-S123-KEG 20 mL (20/box)
5g 8B-S123-LFF 60 mL (16/box)
96-Well Plate
10 mg 8E-S123-AGB 2 Plates/Box
30 mg 8E-S123-TGB 2 Plates/Box
60 mg 8E-S123-UGB 2 Plates/Box
96-Well Microelution Plate
2 mg 8M-S123-4GA ea

**Tab-less tubes available. Contact Phenomenex for details.

Create a customized SPE method in under 1 minute.

For Large Volume Cleanup use Giga Tubes
For SPE Vacuum Manifolds and Accessories, see pp. 79-82 www.phenomenex.com/mdtool

Phenomenex 67
 Strata -X Polymeric SPE ™

U.S. Patent No. 7,119,145

Strata-X-AW and Strata-XL-AW
A weak anion-exchange functionalized polymeric sorbent that allows for complete
retention of acidic compounds with pKa less than 5, making 100 % organic wash

)
conditions possible.
NH2
Strata-X-AW, 33 µm, 85 Å Strata-XL-AW, 100 µm, 300 Å
NH

)
High Concentration Samples X
Small Target Analytes (< 10 kDa) X
Large Target Analytes (> 10 kDa) X
Large Volume Samples X

)
Viscous Samples X

3 Mechanisms of Retention
) NH
NH2 NH
NH2

)
)
Weak Anion-Exchange π-π Bonding Hydrophobic Interaction

)
)

NH2 NH2 NH2

NH NH NH

)
)

Strata-X-AW Strata-XL-AW
Ordering Information Ordering Information
)

Format Sorbent Mass Part Number Unit Format Sorbent Mass Part Number Unit
STRATA-X POLYMERIC SPE | SAMPLE PREPARATION

Tube NH2 Tube NH2

30 mg NH 8B-S038-TAK** 1 mL (100/box) NH 30 mg 8B-S051-TAK 1 mL (100/box)
)

30 mg 8B-S038-TBJ 3 mL (50/box) 60 mg 8B-S051-UBJ 3 mL (50/box)

60 mg 8B-S038-UBJ 3 mL (50/box) 100 mg 8B-S051-EBJ 3 mL (50/box)
100 mg 8B-S038-EBJ 3 mL (50/box) 100 mg 8B-S051-ECH 6 mL (30/box)
100 mg 8B-S038-ECH 6 mL (30/box) 200 mg 8B-S051-FBJ 3 mL (50/box)
)

200 mg 8B-S038-FBJ 3 mL (50/box) 200 mg 8B-S051-FCH 6 mL (30/box)

200 mg 8B-S038-FCH 6 mL (30/box) 500 mg 8B-S051-HCH 6 mL (30/box)
500 mg NH
NH2
8B-S038-HBJ 3 mL (50/box) Giga Tube
)

500 mg 8B-S038-HCH 6 mL (30/box) 2g 8B-S051-KEG 20 mL (20/box)

Giga™ Tube
500 mg 8B-S038-HDG 12 mL (20/box)
1g 8B-S038-JDG 12 mL (20/box)
1g 8B-S038-JEG 20 mL (20/box)
5g 8B-S038-LFF 60 mL (16/box)
96-Well Plate
10 mg 8E-S038-AGB 2 Plates/Box
30 mg 8E-S038-TGB 2 Plates/Box
60 mg 8E-S038-UGB 2 Plates/Box
96-Well Microelution Plate
2 mg 8M-S038-4GA ea

**Tab-less tubes available. Contact Phenomenex for details.

Create a customized SPE method in under 1 minute.

For Large Volume Cleanup use Giga Tubes
For SPE Vacuum Manifolds and Accessories, see pp. 79-82 www.phenomenex.com/mdtool

68 Phenomenex
Strata -X Polymeric SPE ™

U.S. Patent No. 7,119,145

Strata-X-Drug B and Strata-X-Drug N
Now In-Well Hydrolysis
Proprietary sorbents that are designed and quality controlled for basic and neutral drugs of
Capabilities!
abuse analysis. These sorbents do not require a conditioning/equilibrating step.

Strata-X-Drug B Plus Protocol

Pre-treatment 1 2 3
Urine INITIATE VACUUM WASH ELUTE ANALYTE
Load β-Glucuronidase to start SPE method.
urine and β-Glucuronidase Ensure pH is between 4 - 6. Elution
Wash Solvent Solvent
enzyme to start incubation.
Wait 30 min - 3 hours.

Impurities are
washed off Analyte
the sorbent elutes

Strata-X-Drug B Starting Methods Strata-X-Drug N Starting Methods

1 2 3 1 2
Opiates, 6-MAM, PCP,
Amphetamines,
Methadone, Healthcare Marijuana Metabolites Cocaine Metabolites Barbiturates Benzodiazepines
Opiates, and Propoxy-

SAMPLE PREPARATION
phene*
Condition Not Required Condition Not Required
Load Pre-treated urine sample Pre-treated urine sample Pre-treated urine sample Load Pre-treated urine sample Pre-treated urine sample
Wash 1 600 µL of 100 mM 600 µL of 100 mM 600 µL of 0.1 N Wash 1 600 µL of 0.1 N Hydrochloric 600 µL of Acetonitrile/Water (20:80)
Sodium acetate Sodium acetate Hydrochloric acid acid (HCI)
buffer (pH 5.0) buffer (pH 5.0)
Wash 2 2x 600 µL of Methanol/ ––
Wash 2 600 µL Methanol 600 µL of Acetoni- 600 µL Methanol 0.1 N HCI (30:70)
trile/100 mM Sodium

|
acetate buffer (pH 5.0) Dry 10 minutes under full vacuum 10 minutes under full vacuum
(30:70)

STRATA-X POLYMERIC SPE

Elute 2x 300 µL of Ethyl acetate/ 2x 300 µL of Ethyl acetate/
Dry 10 minutes under full 15 minutes under full 10 minutes under full Isopropanol (85:15) Isopropanol (85:15)
vacuum vacuum vacuum
Methods are written for 30 mg/well Strata-X-Drug N 96-well plate; however they can be scaled to
Elute 2x 300 µL of Ethyl 2x 300 µL of Ethyl 2x 300 µL of Ethyl accomodate smaller or larger sample sizes and sorbent masses.
acetate/ acetate/Isopropanol acetate/Isopropanol/
Isopropanol/ Ammonium (85:15) Ammonium
hydroxide (70:20:10) hydroxide (70:20:10)
* Opiates, 6-MAM, PCP, Amphetamines, Methadone, Healthcare Opiates, and Propoxyphene can be
extracted simultaneously or separately using the same SPE methodology.
Strata-X-Drug B Plus
Ordering Information
Methods are written for 30 mg/well Strata-X-Drug B 96-well plate; however they can be scaled to Format Sorbent Mass Part Number Unit
accommodate smaller or larger sample sizes and sorbent masses.
96-Well Plate
10 mg 8E-S128-AGB-P 2 Plates/box
Strata-X-Drug B 30 mg 8E-S128-TGB-P 2 Plates/box
Ordering Information
Format Sorbent Mass Part Number Unit
Tube
10 mg 8B-S128-AAK 1 mL (100/box) Strata-X-Drug N
30 mg 8B-S128-TAK 1 mL (100/box) Ordering Information
30 mg 8B-S128-TBJ 3 mL (50/box) Format Sorbent Mass Part Number Unit
60 mg 8B-S128-UBJ 3 mL (50/box) Tube
60 mg 8B-S128-UCH 6 mL (30/box) 30 mg 8B-S129-TAK 1 mL (100/box)
60 mg 8B-S128-UCL 6 mL (200/bag) 30 mg 8B-S129-TBJ 3 mL (50/box)
Giga™ Tube 60 mg 8B-S129-UBJ 3 mL (50/box)
100 mg 8B-S128-EDG 12 mL (20/box) 60 mg 8B-S129-UCH 6 mL (30/box)
60 mg 8B-S129-UCL 6 mL (200/bag)
96-Well Plate 100 mg 8B-S129-ECH 6 mL (30/box)
10 mg 8E-S128-AGB 2 Plates/box 96-Well Plate
30 mg 8E-S128-TGB 2 Plates/box 10 mg 8E-S129-AGB 2 Plates/box
60 mg 8E-S128-UGB 2 Plates/box 30 mg 8E-S129-TGB 2 Plates/box

Phenomenex 69
 Strata  Solid Phase Extraction (SPE)
™
Solid Phase Extraction

Strata Traditional Solid Phase

Extraction (SPE) Sorbents
Material Characteristics
Particle Size Pore Size Surface Area Carbon Load Ionic Capacity
Phase (µm) (Å) (m2/g) (%) Bonding End Capping (meq/g)
Reversed Phase
C18-E 55 70 500 18.0 trifunctional Yes —
C18-U 55 70 500 17.0 trifunctional No —
C18-T 55 140 300 15.0 trifunctional Yes —
C8 55 70 500 10.5 trifunctional Yes —
Phenyl 55 70 500 10.5 trifunctional Yes —
Normal Phase
CN 55 70 500 10.0 trifunctional No —
NH2 50 60 490 6.5 trifunctional No 1.3
Silica (Si-1) 60 70 490 0.0 — — —
Ion-Exchange
SCX 60 70 500 6.0 trifunctional No 0.2
WCX 55 70 500 8.0 trifunctional No 0.8
SAX 55 70 500 6.5 trifunctional No 0.9
Mixed-Mode
Screen-C GF 200 70 500 proprietary trifunctional — —
Screen-C 55 70 500 proprietary trifunctional — —
Basic Screen Large Reservoir 50 60 600 proprietary trifunctional — —
Cartridge (LRC)
Screen-A 55 70 500 proprietary trifunctional — —
ABW 55 70 500 7.0 — — —
Specialty
STRATA SOLID PHASE EXTRACTION (SPE) | SAMPLE PREPARATION

FL (Florisil®) 170 80 300 0.0 — — —

EPH (Extractable Petroleum 120 30 proprietary 0.0 — — —
Hydrocarbon)
AL-N (Alumina-Neutral) 120 60 165 — — — —
SDB-L 100 260 500 — — — —
Eco-Screen proprietary proprietary proprietary — — — —
Melamine proprietary proprietary proprietary proprietary — — —
PAH proprietary proprietary proprietary proprietary — — —
GCB (Graphitized Carbon Black) proprietary proprietary 70-100 proprietary proprietary proprietary proprietary
PFAS (WAX/GCB) proprietary proprietary proprietary proprietary proprietary proprietary proprietary
Activated Carbon proprietary proprietary proprietary proprietary proprietary proprietary proprietary

Determine the Correct Sorbent Mass

Silica-Based Sorbents
(Strata C18-E, C8, SCX, SAX, WCX, NH2, etc.)
Sample Matrix Sorbent Mass
Blood, serum, plasma 50 mg sorbent per 250 µL
Urine 50 mg sorbent per 500 µL
Filtered tissue homogenates 100 mg sorbent per 100 mg tissue
Environmental Samples Sorbent Mass
Water (particulate-free) drinking 500 mg/100 mL - 500 mL sample
Water (particulate-laden) rivers, runoff, etc. 1 g/100 mL - 500 mL sample
Soil Extracts 1 g/100 g of soil extract

Determine the Correct Sorbent Wash and Elution Volumes

10 mg 50 mg 100 mg 150 mg 200 mg 500 mg 1g 2g 5g 10 g

Silica-Based Sorbent Mass
Practical Minimum Wash and Elution
60 µL 300 µL 600 µL 900 µL 1.2 mL 3 mL 6 mL 12 mL 30 mL 60 mL
Volume 4 bed volumes
Recommended Wash and
120 µL 600 µL 1.2 mL 1.8 mL 2.4 mL 6 mL 12 mL 24 mL 60 mL 120 mL
Elution Volume 8 bed volumes

Create a customized SPE method in under 1 minute.

www.phenomenex.com/mdtool

70 Phenomenex
Strata  Solid Phase Extraction (SPE)
™

Reversed Phase Sorbents

C18-E C18-U
End-capped C18 sorbent that offers strong hydrophobic C18 sorbent with no end-capping, giving the phase moderate
retention with negligible secondary polar interactions from hydrophobic selectivity with slight polar selectivity due to the
active silanol groups. active silanol groups.

O O
O O
Si Si

O O
O O
Si Si Si OH
O O
O

Ordering Information Ordering Information

Sorbent Sorbent
Format Mass Part Number Unit Format Mass Part Number Unit
Tube Tube
50 mg 8B-S001-DAK 1 mL (100/box) 100 mg 8B-S002-EAK 1 mL (100/box)
100 mg 8B-S001-EAK** 1 mL (100/box) 200 mg 8B-S002-FBJ 3 mL (50/box)
100 mg 8B-S001-EBJ 3 mL (50/box) 500 mg 8B-S002-HBJ 3 mL (50/box)

SAMPLE PREPARATION
200 mg 8B-S001-FBJ** 3 mL (50/box) 500 mg 8B-S002-HCH 6 mL (30/box)
200 mg 8B-S001-FCH 6 mL (30/box) 1g 8B-S002-JCH 6 mL (30/box)
500 mg 8B-S001-HBJ 3 mL (50/box) 96-Well Plate
500 mg 8B-S001-HCH 6 mL (30/box) 50 mg 8E-S002-DGB 2 Plates/Box
1g 8B-S001-JEG 20 mL (20/box) 100 mg 8E-S002-EGB 2 Plates/Box
Giga™ Tube
500 mg 8B-S001-HDG 12 mL (20/box)
2g 8B-S001-KDG 12 mL (20/box)

|
5g 8B-S001-LEG 20 mL (20/box)
10 g 8B-S001-MFF 60 mL (16/box)

STRATA SOLID PHASE EXTRACTION (SPE)

20 g 8B-S001-VFF 60 mL (16/box) C18-T
50 g 8B-S001-YSN 150 mL (8/box)
A wide-pore C18 sorbent that offers strong hydrophobic selectivity
70 g 8B-S001-ZSN 150 mL (8/box)
96-Well Plate
and accommodates molecules up to 75 kD in size.
25 mg 8E-S001-CGB 2 Plates/Box
50 mg 8E-S001-DGB 2 Plates/Box
100 mg 8E-S001-EGB 2 Plates/Box
O
O
Si
On-line Extraction Cartridge
Description Part Number Unit/Box O
O
Strata C18-E on-line extraction 00M-S039-B0-CB ea Si Si
cartridge, 20 x 2.0 mm O O
Cartridge holder, 20 mm CH0-5845 ea

Ordering Information
**Tab-less tubes available. Contact Phenomenex for details.
Sorbent
Format Mass Part Number Unit
For Large Volume Cleanup use Giga Tubes Tube
For SPE Vacuum Manifolds and Accessories, see pp. 79-82 100 mg 8B-S004-EAK 1 mL (100/box)
200 mg 8B-S004-FBJ 3 mL (50/box)
500 mg 8B-S004-HBJ 3 mL (50/box)
500 mg 8B-S004-HCH 6 mL (30/box)
1g 8B-S004-JCH 6 mL (30/box)
96-Well Plate
50 mg 8E-S004-DGB 2 Plates/Box

Phenomenex 71
 Strata  Solid Phase Extraction (SPE)
™

Reversed Phase Sorbents

C8 Phenyl
An end-capped C8 sorbent that offers moderate hydrophobic A short alkyl chain with a phenyl group provides moderate
retention with negligible secondary polar interactions from active hydrophobic selectivity and aromatic selectivity through π-π
silanol groups. interactions.

O
O O
Si
O
O Si
O
Si Si O
O O
O Si Si
O O

Ordering Information Ordering Information

Sorbent Sorbent
Format Mass Part Number Unit Format Mass Part Number Unit
STRATA SOLID PHASE EXTRACTION (SPE) | SAMPLE PREPARATION

Tube Tube
100 mg 8B-S005-EAK 1 mL (100/box) 100 mg 8B-S006-EAK 1 mL (100/box)
200 mg 8B-S005-FBJ 3 mL (50/box) 200 mg 8B-S006-FBJ 3 mL (50/box)
500 mg 8B-S005-HBJ 3 mL (50/box) 500 mg 8B-S006-HBJ 3 mL (50/box)
500 mg 8B-S005-HCH 6 mL (30/box) 500 mg 8B-S006-HCH 6 mL (30/box)
1g 8B-S005-JCH 6 mL (30/box) 1g 8B-S006-JCH 6 mL (30/box)
Giga™ Tube 96-Well Plate
2g 8B-S005-KDG 12 mL (20/box) 25 mg 8E-S006-CGB 2 Plates/Box
5g 8B-S005-LEG 20 mL (20/box) 100 mg 8E-S006-EGB 2 Plates/Box
10 g 8B-S005-MFF 60 mL (16/box)
96-Well Plate
25 mg 8E-S005-CGB 2 Plates/Box

On-line Extraction Cartridge

Description Part Number Unit/Box
Strata C8 on-line extraction 00M-S101-B0-CB ea
cartridge, 20 x 2.0 mm
Cartridge holder, 20 mm CH0-5845 ea
RP
(proprietary reversed phase on-line
extraction column)
On-line Extraction Column
Description Part Number Unit/Box
50 x 1.0 mm 00B-S326-A0 ea
50 x 0.5 mm 00B-S326-AF ea

For Large Volume Cleanup use Giga Tubes On-line Extraction Cartridge
For SPE Vacuum Manifolds and Accessories, see pp. 79-82 Description Part Number Unit/Box
20 x 2.1 mm 00M-S326-AN ea
Cartridge holder, 20 mm CH0-5845 ea

Don't see the size or format you want? Contact Phenomenex

or your local distributor for other dimensions, Giga tubes, and
bulk sorbent pricing, and part numbers.

72 Phenomenex
Strata  Solid Phase Extraction (SPE)
™

Normal Phase Sorbents

Silica NH2/WAX
(Si-1)
Unbonded silica particle that offers strong polar selectivity. This amino phase offers strong polar selectivity and hydrogen
bonding under normal phase conditions or can be used as a weak
anion-exchange sorbent.

O O
O O
Si OH Si NH2

O O
O O
Si OH Si NH2
O O

Ordering Information Ordering Information

SAMPLE PREPARATION
Sorbent Sorbent
Format Mass Part Number Unit Format Mass Part Number Unit
Tube Tube
100 mg 8B-S012-EAK** 1 mL (100/box) 100 mg 8B-S009-EAK 1 mL (100/box)
200 mg 8B-S012-FBJ 3 mL (50/box) 200 mg 8B-S009-FBJ 3 mL (50/box)
500 mg 8B-S012-HBJ** 3 mL (50/box) 500 mg 8B-S009-HBJ 3 mL (50/box)
500 mg 8B-S012-HCH 6 mL (30/box) 500 mg 8B-S009-HCH 6 mL (30/box)
1g 8B-S012-JCH** 6 mL (30/box) 1g 8B-S009-JCH 6 mL (30/box)
Giga™ Tube Giga Tube

|
500 mg 8B-S012-HDG 12 mL (20/box) 500 mg 8B-S009-HDG 12 mL (20/box)

STRATA SOLID PHASE EXTRACTION (SPE)

1g 8B-S012-JDG 12 mL (20/box) 2g 8B-S009-KDG 12 mL (20/box)
2g 8B-S012-KDG 12 mL (20/box) 5g 8B-S009-LEG 20 mL (20/box)
5g 8B-S012-LEG 20 mL (20/box) 10 g 8B-S009-MFF 60 mL (16/box)
10 g 8B-S012-MFF 60 mL (16/box) 20 g 8B-S009-VFF 60 mL (16/box)
20 g 8B-S012-VFF 60 mL (16/box) 96-Well Plate
96-Well Plate 25 mg 8E-S009-CGB 2 Plates/Box
50 mg 8E-S012-DGB 2 Plates/Box 50 mg 8E-S009-DGB 2 Plates/Box
100 mg 8E-S012-EGB 2 Plates/Box 100 mg 8E-S009-EGB 2 Plates/Box

**Tab-less tubes available. Contact Phenomenex for details.

Cyano
(CN) Ordering Information
A polar phase with slight hydrophobic selectivity in reversed phase Sorbent
mode and moderate polar selectivity in normal phase mode. Format Mass Part Number Unit
Tube
100 mg 8B-S007-EAK 1 mL (100/box)
200 mg 8B-S007-FBJ 3 mL (50/box)
O 500 mg 8B-S007-HBJ 3 mL (50/box)
500 mg 8B-S007-HCH 6 mL (30/box)
O 1g 8B-S007-JCH 6 mL (30/box)
Si C
Giga Tube
N
O 2g 8B-S007-KDG 12 mL (20/box)

O
Si OH 96-Well Plate
50 mg 8E-S007-DGB 2 Plates/Box
O

Phenomenex 73
 Strata  Solid Phase Extraction (SPE)
™

Ion-Exchange Sorbents

SAX WCX
(strong anion-exchange) (weak cation-exchange)
The quaternary amine phase remains positively charged under A carboxylic acid group is bonded to the surface of the silica
all conditions, giving a strong anion-exchange mechanism of particle, giving a weak cation-exchange selectivity.
retention.
O
O O
O
Si N Cl- O
Si OH

O O
O O
Si OH Si OH
O O

Ordering Information Ordering Information

Sorbent Sorbent
Format Mass Part Number Unit Format Mass Part Number Unit
Tube Tube
100 mg 8B-S008-EAK 1 mL (100/box) 100 mg 8B-S027-EAK 1 mL (100/box)
100 mg 8B-S008-EBJ 3 mL (50/box) 200 mg 8B-S027-FBJ 3 mL (50/box)
STRATA SOLID PHASE EXTRACTION (SPE) | SAMPLE PREPARATION

200 mg 8B-S008-FBJ 3 mL (50/box) 500 mg 8B-S027-HBJ 3 mL (50/box)

500 mg 8B-S008-HBJ 3 mL (50/box) 500 mg 8B-S027-HCH 6 mL (30/box)
500 mg 8B-S008-HCH 6 mL (30/box) 1g 8B-S027-JCH 6 mL (30/box)
1g 8B-S008-JCH 6 mL (30/box) Giga Tube
Giga™ Tube 2g 8B-S027-KDG 12 mL (20/box)
500 mg 8B-S008-HDG 12 mL (20/box) 5g 8B-S027-LEG 20 mL (20/box)
2g 8B-S008-KDG 12 mL (20/box) 96-Well Plate
5g 8B-S008-LEG 20 mL (20/box) 25 mg 8E-S027-CGB 2 Plates/Box
20 g 8B-S008-VFF 60 mL (16/box) 50 mg 8E-S027-DGB 2 Plates/Box
96-Well Plate
25 mg 8E-S008-CGB 2 Plates/Box
50 mg 8E-S008-DGB 2 Plates/Box
100 mg 8E-S008-EGB 2 Plates/Box

SCX
Ordering Information
(strong cation-exchange) Sorbent
A benzene sulfonic acid group is bonded to the surface of the silica Format Mass Part Number Unit
Tube
particle, giving strong cation-exchange selectivity.
100 mg 8B-S010-EAK 1 mL (100/box)
100 mg 8B-S010-EBJ 3 mL (50/box)
200 mg 8B-S010-FBJ 3 mL (50/box)
500 mg 8B-S010-HBJ 3 mL (50/box)
O 500 mg 8B-S010-HCH 6 mL (30/box)
O 1g 8B-S010-JCH 6 mL (30/box)
O
Si Giga Tube
O H 2g 8B-S010-KDG 12 mL (20/box)
S
O 5g 8B-S010-LEG 20 mL (20/box)
10 g 8B-S010-MFF 60 mL (16/box)
O
Si O 20 g 8B-S010-VFF 60 mL (16/box)
OH
96-Well Plate
O 25 mg 8E-S010-CGB 2 Plates/Box
50 mg 8E-S010-DGB 2 Plates/Box
100 mg 8E-S010-EGB 2 Plates/Box

74 Phenomenex
Strata  Solid Phase Extraction (SPE)
™

Mixed-Mode Sorbents

Screen-C ABW
Incorporates the hydrophobic selectivity of a C8 phase and strong Offers a strong cation-exchange group and a weak anion-exchange
cation-exchange for the extraction of basic drugs from biological group for the extraction or fractionation of complex mixtures.
matrixes.
O O
O O H O O H
S S
O O
Si Si
O O
O O
O O
Si Si
O O NH2

Ordering Information Ordering Information

Sorbent Sorbent
Format Mass Part Number Unit Format Mass Part Number Unit
Tube Tube
100 mg 8B-S016-EAK** 1 mL (100/box) 200 mg 8B-S030-FBJ 3 mL (50/box)
100 mg 8B-S016-EBJ 3 mL (50/box) 1g 8B-S030-JCH 6 mL (30/box)

SAMPLE PREPARATION
150 mg 8B-S016-SBJ 3 mL (50/box) Giga Tube
150 mg 8B-S016-SCH 6 mL (30/box) 2g 8B-S030-KDG 12 mL (20/box)
200 mg 8B-S016-FBJ 3 mL (50/box) 5g 8B-S030-LEG 20 mL (20/box)
200 mg 8B-S016-FCH 6 mL (30/box)
300 mg 8B-S016-RBJ 3 mL (50/box)
300 mg 8B-S016-RCH 6 mL (30/box)
500 mg 8B-S016-HCH 6 mL (30/box)
96-Well Plate
50 mg 8E-S016-DGB 2 Plates/Box

|
STRATA SOLID PHASE EXTRACTION (SPE)
Screen-C GF Screen-A
Offers the selectivity of Screen-C in a gravity flow particle size for Incorporates the hydrophobic selectivity of a C8 phase and strong
viscous samples. anion-exchange for the extraction of acidic drugs from biological
matrixes.
Ordering Information
Sorbent
Format Mass Part Number Unit
Tube O
500 mg 8B-S026-HBJ 3 mL (50/box)
O N Cl
Si

O
Basic Screen Large Reservoir Cartridge O
(LRC) Si
Improved recovery of basic drugs from biological samples in a O
funnel shaped tube ideal for large sample volumes with minimal
extraction solvents.
Ordering Information Ordering Information
Sorbent Sorbent
Format Mass Part Number Unit Format Mass Part Number Unit
Tube Tube
Large Reservoir 200 mg 8B-S327-FTH 10 mL (30/box) 100 mg 8B-S019-EAK 1 mL (100/box)
Cartridge 200 mg 8B-S019-FBJ 3 mL (50/box)
200 mg 8B-S019-FCH 6 mL (30/box)
500 mg 8B-S019-HCH 6 mL (30/box)
**Tab-less tubes available. Contact Phenomenex for details.

Phenomenex 75
 Strata  Solid Phase Extraction (SPE)
™

Specialty Sorbents

Alumina-N Florisil®
(AL-N) (FL)
A polar phase that allows for the extraction of polar compounds A modified silica sorbent that contains a magnesium ion, allowing
from food and environmental samples. for the retention of polar and halogenated compounds, like pesti-
cides, from environmental samples.

Ordering Information Ordering Information

Sorbent Sorbent
Format Mass Part Number Unit Format Mass Part Number Unit
Tube Tube
500 mg 8B-S313-HBJ 3 mL (50/box) 500 mg 8B-S013-HBJ 3 mL (50/box)
1g 8B-S313-JCH 6 mL (30/box) 500 mg 8B-S013-HCH 6 mL (30/box)
Giga™ Tube 1g 8B-S013-JCH 6 mL (30/box)
2g 8B-S313-KDG 12 mL (20/box) 2.5 g 8B-S013-8CH 6 mL (30/box)
Giga Tube
2g 8B-S013-KDG 12 mL (20/box)
5g 8B-S013-LEG 20 mL (20/box)
10 g 8B-S013-MFF 60 mL (16/box)

Eco-Screen Melamine
This proprietary normal phase sorbent is topped with sodium sul- A proprietary phase that allows for the simultaneous extraction of
STRATA SOLID PHASE EXTRACTION (SPE) | SAMPLE PREPARATION

fate to remove any excess water and used for the extraction of melamine and cyanuric acid out of food and biological samples.
hydrocarbons from environmental samples, resulting in high recov-
eries of naphthalene.

Ordering Information Ordering Information

Sorbent Sorbent
Format Mass Part Number Unit Format Mass Part Number Unit
Tube Tube
1 g 8B-S046-JBJ 3 mL (50/box) 100 mg 8B-S049-EBJ 3 mL (50/box)
200 mg 8B-S049-FBJ 3 mL (50/box)

GCB PFAS
(graphitized carbon black) (WAX/GCB)
This high quality non-porous graphitized carbon offers bet- Consists of a stacked single cartridge solution filled with polymeric
ter retention of polar compounds compared to C8 or C18 silica WAX (200 mg) and GCB sorbents (50 mg) that functions to meet the
products making it ideal for pesticide and PFAS extraction or DOD guidelines for PFAS testing. It is ideal for complex biota matri-
pigment clean-up. ces and reduces the need for multiple extraction tubes.

Ordering Information Ordering Information

Sorbent Format Sorbent Mass Part Number Unit
Format Mass Part Number Unit Tube
Tube 200 mg / 50 mg CS0-9207 6 mL (30/box)
200 mg 8B-S528-FCH 6 mL (30/box) 500 mg/ 50 mg CS0-9208 6 mL (200/box)
500 mg 8B-S528-HCH 6 mL (30/box) 50 mg / 200 mg CS0-9214 6 mL (300/box)

Activated Carbon (NEW)

A high surface area fully porous sorbent that offers a better reten- Ordering Information
tion of polar compounds compared to C8 and C18 silica products. Format Sorbent Mass Part Number Unit
Designed and manufactured from high quality chromatography- Tube
grade porous carbon. Improved recovery when working with high 400 mg CS0-9210 Pass Through Cartridge (50/box)
polar analytes from aqueous matrices. 2 g CS0-9209 6 mL (30/box)

76 Phenomenex
Strata  Solid Phase Extraction (SPE)
™

Specialty Sorbents

SDB-L EPH
(styrene-divinylbenzene) (Extractable Petroleum Hydrocarbons)
A rugged polymer sorbent that is pH stable from 1-14 and offers hy- This specialty normal phase sorbent was developed for the
drophobic and aromatic selectivity for reversed phase applications. fractionation of aliphatic and aromatic hydrocarbons from
environmental samples.

O
n O
Si OH
)

O
O
Si
)

OH
O

Ordering Information Ordering Information

Sorbent Sorbent
Format Mass Part Number Unit Format Mass Part Number Unit
Tube Tube
100 mg 8B-S014-EAK 1 mL (100/box) 500 mg 8B-S031-HBJ 3 mL (50/box)

SAMPLE PREPARATION
200 mg 8B-S014-FBJ 3 mL (50/box)
Giga Tube
200 mg 8B-S014-FCH 6 mL (30/box)
5g 8B-S031-LEG 20 mL (20/box)
500 mg 8B-S014-HBJ 3 mL (50/box)
500 mg 8B-S014-HCH 6 mL (30/box)
1g 8B-S014-JCH 6 mL (30/box) Teflon® Giga Tube
Giga Tube
™ 5g 8B-S031-LEG-T 20 mL (20/box)
10 g 8B-S014-MFF 60 mL (16/box)

96-Well Plate

|
50 mg 8E-S014-DGB 2 Plates/Box

STRATA SOLID PHASE EXTRACTION (SPE)

PAH
(Polycyclic Aromatic Hydrocarbons) Sodium Sulfate
This proprietary sorbent was designed to provide high A specialized sorbent that is used for the removal of
recoveries of polycyclic aromatic hydrocarbons from water aqueous residues from organic solutions in an effort to
(as specified in EPA Method 550.1) while simultaneously reduce blow-down time.
removing humic acids from the extract.

Ordering Information Ordering Information

Sorbent Sorbent
Format Mass Part Number Unit Format Mass Part Number Unit
Tube Tube
500 mg 8B-S130-HCH 6 mL (30/box) 1g 8B-S124-JCH 6 mL (30/box)
750 mg 8B-S130-WCH 6 mL (30/box)
Giga Tube
1.5 g 8B-S130-7CH 6 mL (30/box)
5g 8B-S124-LEG 20 mL (20/box)

Don’t see the size or format you want? Contact Phenomenex or

your local distributor for other dimensions, Giga tubes, and bulk
sorbent pricing and part numbers.

Phenomenex 77
 Biozen ™
Biological Sample Clean-up

Biozen MagBeads
Streptavidin Coated Material Characteristics
Outside Biotin
Uniform and efficient magnetic particles result in faster and reli- Bead Bead Coating Binding Coating Available
Type Diameter Type Capacity Specification Concentration Formats
able purification, clean-up, and isolation of proteins and peptide
Iron coated 1 µm Streptavidin > 200 pmol Tosyl- 20 mg/mL 25 mg,
molecules. Biotin/mg activated, 50 mg,
blocked with 500 mg
hydrophilic
• Excellent for binding biotinylated capture antibodies copolymer
• 1.0 µm Streptavidin coated magnetic beads
• Available in 25 mg, 50 mg, and 500 mg formats

Area Ratio, Rituximab

150

Thermo Scientific® Dynabeads™ M-280 Blocked with

hydrophilic copolymer
Biozen MagBeads, Lot 1
Biozen MagBeads, Lot 2
100 Polymeric shell

Streptavidin coating coupled

Area Ratio

with biotinylated BSA

Iron oxide paramagnetic nanoparticles
ANALYSIS OF BIOLOGICS | BIOZEN | SAMPLE PREPARATION

and polymeric core

50

Immunocapture Bead Correlation Coefficient

M-280 0.9176
Biozen MagBeads, Lot 1 0.9914
Biozen MagBeads, Lot 2 0.9941
0
0 10 Concentration (µg/mL) 20 30

Ordering Information
Product Coating Formats Part No. Concentration Bead Size
Biozen MagBeads Streptavidin 25 mg (1.25 mL) KS0-9531 20 mg/mL 0.95-1.15 µm
50 mg (2.5 mL) KS0-9532
500 mg (25 mL) KS0-9533

Learn More:
www.phenomenex.com/BiozenMagBeads

N-Glycan Clean-Up
HILIC Solid Phase Extraction (SPE)
High recovery of labeled released N-glycans in a microelution format allowing
for streamlined processing and clean-up of small sample volumes.

Ordering Information
Biozen Solid Phase Extraction Format Sorbent Mass Part No. Unit
Biozen N-Glycan Clean-Up Microelution 96-Well Plate 5 mg/well 8M-S009-NGA 1/box

78 Phenomenex
Sample Preparation Accessories
Presston 1000 Positive Pressure Manifold
Your Newest Solution to Increased Productivity
Presston 1000 is a positive pressure manifold designed to make
sample preparation processing easy and consistent. It applies Compatible with:
pressure from above to push liquid through sample preparation
sorbents to provide uniform flow rates when processing samples. Phospholipid Removal Solutions

Presston 1000 is compatible with standard 96-well plates, including

microelution plates, and can even be used with 1 mL tubes with the
addition of a tabless tube holder (Part No.: AH0-9055). β-Glucuronidase Removal Products simplified liquid extraction

• Pneumatic Handling
• Consistent Flow Rates Solid Phase Extraction Polymeric SPE
A Rapid Solid Phase Extraction Solution

• Safe and Easy-to-Use

Do More with Presston 1000

Streamline your 96-well plate processing for easier sample
preparation with a pneumatic positive pressure manifold.

Sleek, Low Profile Design

Width: 11.8”, Depth: 15”, Height: 14.8”

Simple to Use

SAMPLE PREPARATION
Pneumatic manifold movement reduces
manual labor with more reliable extractions

Never Lose Pressure

Always maintain a tight seal between
the manifold and 96-well plate

Determine Your Operating Pressure

Conveniently monitor and maintain a

|
consistent pressure

PRESSTON
Easily Load Samples Know your Step
Moveable locator plate makes sample Simply move the “SPE Procedure Indicator”
loading and cleaning easy to the correct step to stay on track of your
extraction.

Safer Lab Environment

Use both hands to move manifold shield,
ensuring enhanced safety

Ensure Reliability Ordering Information

Phenomenex warrants the Presston 1000 Positive Pressure Part No. Description Unit
Manifold against defects in materials and workmanship
Presston 1000 Positive Pressure Manifold
under normal installation, use, and maintenance for a
period of 12 months following delivery. Please visit AH1-7033 SPE Positive Pressure Manifold, 96-Well Plate, ea
www.phenomenex.com/presstonwarranty complete assembly
for complete warranty information.

Phenomenex 79
 Sample Preparation Accessories
Vacuum Manifolds Stopcock

SPE Tube Vacuum Manifold

• Process up to 12 or 24 samples at one time Glass Chamber
• Process up to 10 large volume samples at one time
Collection Rack
• Female Luer inlets fit all male Luer tipped SPE tubes
and cartridges Vacuum Gauge

Ordering Information
Part No. Description Unit Valve Assembly
24 – Position Vacuum Manifold*
VM24 SPE 24-Position Vacuum Manifold Set, ea Space for collection tubes
complete assembly
24 – Position Vacuum Manifold Replacement Parts
A82404 SPE Gasket ea - Fits 13 mm and 16 mm test tubes up to 125 mm in height.
VM24-J SPE Collection Rack ea - A flow rate of 1 – 3 drops per second (1 – 3 mL /min) is recommended
A82411 SPE 24-Position Vacuum Waste Container, polypropylene ea during the loading and elution steps for typical small volume samples
A81213 SPE Luer Stopcocks 12/pk (< 5 mL). At these critical steps the analytes are chemically interacting
12 – Position Vacuum Manifold* with the sorbent.
VM12 SPE 12-Position Vacuum Manifold Set, ea - Large volume samples (> 100 mL) in large cartridges (>1 gram) may be
complete assembly
12 – Position Vacuum Manifold Replacement Parts
processed at flow rates between 5 – 10 mL/minute.
A80106 SPE Gasket ea - Conditioning and Wash steps are generally not flow critical.
A81216 SPE Collection Rack Assembly, including plates, legs and clips ea - Individual stopcocks are typically not needed when using the Strata-X
A81215 SPE 12-Position Vacuum Waste Container, polypropylene ea family of sorbents. They are very forgiving of improper flow rates and
A81213 SPE Luer Stopcocks 12/pk are truly resistant to deconditioning effects caused by excessive drying
ACCESSORIES | SAMPLE PREPARATION

A81217 Legs for 12-Position Cover 4/pk during the method.

- Reversed phase methods are more forgiving of fast flow rates than ion-
exchange or normal phase.

Compatible with:

Phospholipid Removal Solutions β-Glucuronidase Removal Products simplified liquid extraction

A Rapid Solid Phase Extraction Solution

Solid Phase Extraction Polymeric SPE

Reservoir/Empty
Processing Large Sample Volumes Teflon Or Vacuum
Resistant Tubing
Syringe Barrel

Adapter
Cap
Have Large Sample Volume
but Need a Small Bed Mass?
Use an adapter cap to attach
another SPE tube, which can
be used to increase the reser-
voir size for washing or eluting
solvents.
Adapter Cap
Vacuum Waste Vacuum
(A80215) Sample Manifold Trap Pump

Description Part No.

Teflon or Vacuum Resistant Tubing (1/8 inch O.D.) AT0-2956
Adapter Cap A80215

* Manifolds include: Vacuum-tight glass chamber, vacuum gauge assembly, polypropylene lid with gasket, male and female luers and yellow end
plugs, stopcock valves, collection rack assemblies, polypropylene needles, lid support legs. Waste container included with 12-position manifold.

80 Phenomenex
Sample Preparation Accessories
Vacuum Manifolds
96-Well Plate Vacuum Manifold Well Plate

• Includes vacuum valve attachment and two collection

plate spacer inserts
Acrylic Chamber
• Made of durable acrylic (manifold top)
• Designed to accommodate 96-well plates, collection
plates, protein precipitation plates, and filtration plates
Manifold Base Plate
(houses collection plate)

Ordering Information 96-Well Plate Sample

Preparation Product
96-Well Plate Manifold**
Part No. Description Unit
Fully assembled, ready
AH0-8950 96-Well Plate Manifold, ea Collection Plate to cleanup, concentrate,
Universal w/vacuum gauge (under acrylic and/or solvent-switch
Replacement Parts chamber) 96 samples at one time.
Part No. Description Unit
AH0-7285 96-Well Plate Manifold Replacement Gasket, Flat (to fit ea
between acrylic chamber and 96-well plate), black
AH0-7198 96-Well Plate Manifold Replacement Gasket, Profile, (to fit ea
between acrylic chamber and manifold base), white
AH0-8637 Reservoir, Single Well, High Profile, 96 Bottom Troughs 25/pk
**Manifold, compatible with 2 mL Impact plate, Novum SLE 96-well plate, Phree Phospholipid Removal Acrylic Chamber
plate, Strata, and Strata-X 96-well plate formats. Vacuum Gauge

Compatible with:

SAMPLE PREPARATION
Phospholipid Removal Solutions

simplified liquid extraction β-Glucuronidase Removal Products

Collection plate spacers accommodate various

collection plate heights. A Rapid Solid Phase Extraction Solution

Solid Phase Extraction Polymeric SPE

|
ACCESSORIES
Vacuum Manifold Accessoriesfor Tube and 96-Well Plates
Ordering Information
General Vacuum Manifold Accessories
Part No. Description Unit
A80215 Adapter Caps for 1, 3 and 6 mL SPE tubes, polyethylene, 12/pk
with Luer tip
A80100 SPE Manifold Needles, polypropylene 12/pk
Adapter Caps Female Luers Retaining Clips A80102 SPE Manifold Needles, stainless steel 12/pk
(for 1, 3, and 6 mL tubes) A80104 Female Luer Fittings 12/pk
A80105 Male Luer Fittings 12/pk
A01003 Vacuum Gauge and Valve Assembly ea
A80111 Retaining Clips 12/pk
A80117 Plugs/ Dust Caps 12/pk
A81213 Stopcocks 12/pk

Polypropylene Male Luers Plugs

Manifold Needles

Stainless Steel Stopcock

Manifold Needles

Phenomenex 81
 Sample Preparation Accessories
Collection Plates Filtration Plate
• Available in conical V- and round-bottom formats • Available in 0.7 µm membrane porosity
• Made of chemically inert polypropylene • Inert surface eliminates non-specific binding for
• Available in 350 µL, 1 and 2 mL volumes maximized results
• Cost effective solution to meet all filtration goals
Conical V- and round-bottom for maximized
sample delivery Ordering Information
Filtration Plates
Part No. Description Unit/Box
AF0-8300 0.7 μm Glass Fiber 96-Well Filtration Plate 2

(AH0-7194) (AH0-7193) Sealing Mats and Tape

2 mL Square Well 1 mL Square Well • Fits all Phenomenex 96-well plates, square-well collection
plates, round-well collection plates, protein precipitation
plates, and filtration plates
• Pierceable and Pre-Slit available

(AH0-7192) (AH1-7025)
350 μL Square Well 1 mL Round Well

Ordering Information (AH0-7195)

Ordering Information
Collection Plates*
Sealing Mats*
ACCESSORIES | SAMPLE PREPARATION

Part No. Description Unit

Part No. Description Unit
AH0-7192 350 µL/well 96-Square Well Conical V-bottom 50/pk
Collection Plate AH0-8597 Sealing Mats, Pierceable, 96-Square Well, Silicone 50/pk
AH0-7193 1 mL/well 96-Square Well Conical V-bottom 50/pk AH0-8598 Sealing Mats, Pre-Slit, 96-Square Well, Silicone 50/pk
Collection Plate AH0-8631*** Sealing Mats, Pierceable, 96-Round Well 7 mm, Silicone 50/pk
AH1-7025 1 mL/well 96-Round Well Round Bottom 7 mm 50/pk AH0-8632*** Sealing Mats, Pre-Slit, 96-Round Well 7 mm, Silicone 50/pk
Collection Plate AH0-8633** Sealing Mats, Pierceable, 96-Round Well 8 mm, Silicone 50/pk
AH0-7194 2 mL/well 96-Square Well Conical V-bottom 50/pk AH0-8634** Sealing Mats, Pre-Slit, 96-Round Well 8 mm, Silicone 50/pk
Collection Plate AH0-8199 Sealing Mats, Pierceable, 96 Square Well, Santoprene™ 100/pk
AH0-8636 2 mL/well 96-Round Well Round Bottom 8 mm 50/pk AH0-7195 Sealing Mats, Pierceable, 96-Square Well, Ethylene 50/pk
Collection Plate Vinyl Acetate (EVA)
AH0-9332 1.2 mL/well 96-Round Well Round Bottom 50/pk AH0-7362 Sealing Tape Pad 10/pk
Collection Plate
*Square well sealing mats compatible with 2 mL Impact plates, Novum SLE 96-well plate, Phree
AH0-9333 0.5 mL/well 96-Round Well V-Bottom, 7 mm 50/pk Phospholipid Removal plate, Strata and Strata-X 96-well plates, and 96 square well collection plates.
Collection Plate, Sterile
AH0-9341 0.5 mL/well 96-Round Well Conical Bottom 7 mm 50/pk **8 mm round-well sealing mats compatible with 2 mL round-well 8 mm collection plates (AH0-8636)
Collection Plate ***7 mm round-well sealing mats compatible with1 mL round-well 7 mm collection plates (AH0-7279)
AH1-7036 2 mL/well Low-Bind 96-Round Well Conical Bottom (deep 120/pk
well, polypropylene, glass lined) Collection Plate

96-Well Tab-less Tube Holders

• Easily process partial plates
• Arrange multiple SPE sorbents in one plate
• Easily replace a single SPE tube
• Compatible with Strata™ and Strata-X 1 mL
tab-less SPE tubes

(AH0-9054)
Ordering Information
96-Well Tab-less Tube Holders
Part No. Description Unit
AH0-9054 96-Well 1 mL Tab-less Tube Holder for use with the ea
96-Well plate vacuum manifold (AH0-8950)
AH0-9055 96-Well 1 mL Tab-less Tube Holder for use with ea
positive pressure manifolds

82 Phenomenex
roQ  An Easier QuEChERS Solution
™

Why Choose roQ QuEChERS?

Improved with you in mind, roQ picks up where other QuEChERS
kits fail. The unique design of the roQ QuEChERS kits eliminates
common problems seen with current QuEChERS kits on the market.

Ease of Use
Built-in Removable Rack* Stand Alone Extraction Tubes Easy Pour Salt Packets

*Applies to roQ Extraction Kits (excludes dSPE Kits)

SAMPLE PREPARATION
Quality
Leak-Free Tubes Low Leachate Tubes Quality Management System Certified
• Validates processes to be fully
established, functional, and meet
international standards
Abundance
950000
900000
850000
800000
750000
700000

• MSDS and Certificate of Analysis

650000

| ROQ | QUECHERS KITS

600000
550000
500000
450000
400000

(CoA) available for all kits

350000
300000
250000
200000
150000
100000
50000

• roQ QuEChERS kits are guaranteed

Time 4 6 8 10 12 14 16 18 20 22 24 26 28 30 min

15 mL roQ centrifuge tube extracted

for quality
with 1 % Acetic acid in Acetonitrile QUALITY
MANAGEMENT SYSTEM
and then with Toluene.
CERTIFIED BY DNV GL
9001:2015

*Applies to roQ Extraction Kits (excludes dSPE Kits)

Technical Support

Sample Preparation Support at Your Fingertips Free Method Development Services

• Dedicated sample preparation team • Let our specialists help you with new method
available to assist your method development development, method optimization, and
needs validation, including FDA compliant and GMP
• Expertise in sample preparation and solid compliant validation.
phase extraction
• Access to up-to-date sample preparation
applications For more details on roQ QuEChERS Kits:
www.phenomenex.com/roQ

Phenomenex 83
 roQ  An Easier QuEChERS Solution
™

Select Your roQ QuEChERS Kit ( Quick - Easy - Cheap - Effective - Rugged - Safe )
Step 1
Extraction*
QuEChERS can be performed by following 3 different methods:
The AOAC 2007.01 Method, the EN 15662 Method, or the Original
Non-Buffered Method.
Select Your roQ Extraction Kit
AOAC 2007.01 Method Original Non-Buffered Method EN 15662 Method
6.0 g MgSO4, 1.5 g NaOAc 4.0 g MgSO4, 1.0 g NaCl 4.0 g MgSO4, 1.0 g NaCl,
KS0-8911 KS0-8910 1.0 g SCTD, 0.5 g SCDS
6.0 g MgSO4, 1.5 g NaCl KS0-8909
KS0-8912
Step 2
Clean Up/dSPE** AOAC 2007.01 EN 15662

1 mL 8 mL 1 mL 6 mL

General
150 mg MgSO4 1200 mg MgSO4 150 mg MgSO4 900 mg MgSO4
50 mg PSA 400 mg PSA 25 mg PSA 150 mg PSA
KS0-9511 KS0-9515 KS0-9503 KS0-9507
QUECHERS KITS | ROQ | SAMPLE PREPARATION

Fats and Waxes 150 mg MgSO4 1200 mg MgSO4 150 mg MgSO4 900 mg MgSO4

50 mg PSA 400 mg PSA 25 mg PSA 150 mg PSA

50 mg C18E 400 mg C18E 25 mg C18E 150 mg C18E

KS0-9512 KS0-9516 KS0-9504 KS0-9508

Pigmented 150 mg MgSO4 1200 mg MgSO4 150 mg MgSO4 900 mg MgSO4

50 mg PSA 400 mg PSA 25 mg PSA 150 mg PSA

50 mg GCB 400 mg GCB 2.5 mg GCB 15 mg GCB

KS0-9513 KS0-9517 KS0-9505 KS0-9509

Highly Pigmented 150 mg MgSO4 900 mg MgSO4

25 mg PSA 150 mg PSA

— —
7.5 mg GCB 45 mg GCB

KS0-9506 KS0-9510

Pigments and Fats 150 mg MgSO4 1200 mg MgSO4

50 mg PSA 400 mg PSA

50 mg GCB 400 mg GCB — —

50 mg C18E 400 mg C18E

KS0-9514 KS0-9518

*All roQ Extraction kits contain fifty easy-pour salt packets and fifty 50 mL stand-alone centrifuge tubes.
**All roQ dSPE kits contain pre-weighed sorbents/salts inside 2 mL or 15 mL centrifuge tubes.

Salts and Sorbents used in roQ Kits

Extraction: Clean Up/dSPE:

• Magnesium Sulfate (MgSO4 ) • Magnesium Sulfate (MgSO4 )
• Sodium Acetate (NaOAc) • Primary/Secondary Amine (PSA)
• Sodium Chloride (NaCI) • Endcapped C18 Sorbent (C18E)
• Sodium Citrate Tribasic Dihydrate (SCTD) • Graphitized Carbon Black (GCB)
• Sodium Citrate Dibasic Sesquihydrate (SCDS)

84 Phenomenex
roQ  An Easier QuEChERS Solution
™

roQ™ Extraction Kits

Extraction kits contain fifty easy-pour salt packets and fifty 50 mL stand-alone centrifuge tubes
Ordering Information
Description Unit Part No.
AOAC 2007.01 Method Extraction Kits
6.0 g MgSO4, 1.5 g NaOAc 50/pk KS0-8911*
EN 15662 Method Extraction Kits
4.0 g MgSO4, 1.0 g NaCl, 1.0 g SCTD, 0.5 g SCDS 50/pk KS0-8909*
Original Non-Buffered Method Extraction Kits
4.0 g MgSO4, 1.0 g NaCl 50/pk KS0-8910
6.0 g MgSO4, 1.5 g NaCl 50/pk KS0-8912
*AOAC and EN Extraction Kits also available in traditional non-collared 50 mL centrifuge tubes, Part No.: KS0-8911-NC and KS0-8909-NC

roQ dSPE Kits

dSPE kits contain pre-weighed sorbents/salts inside 2 mL or 15 mL centrifuge tubes
Ordering Information
Description Unit Part No.
2 mL dSPE Kits
150 mg MgSO4, 25 mg PSA, 25 mg C18E 100/pk KS0-9504
150 mg MgSO4, 25 mg PSA, 2.5 mg GCB 100/pk KS0-9505
150 mg MgSO4, 25 mg PSA, 7.5 mg GCB 100/pk KS0-9506
150 mg MgSO4, 25 mg PSA 100/pk KS0-9503
150 mg MgSO4, 50 mg PSA, 50 mg C18E, 50 mg GCB 100/pk KS0-9514
150 mg MgSO4, 50 mg PSA, 50 mg C18E 100/pk KS0-9512
150 mg MgSO4, 50 mg PSA, 50 mg GCB 100/pk KS0-9513
150 mg MgSO4, 50 mg PSA 100/pk KS0-9511

SAMPLE PREPARATION
15 mL dSPE Kits
900 mg MgSO4, 150 mg PSA, 150 mg C18E 100/pk KS0-9508
900 mg MgSO4, 150 mg PSA, 15 mg GCB 100/pk KS0-9509
900 mg MgSO4, 150 mg PSA, 45 mg GCB 100/pk KS0-9510
900 mg MgSO4, 150 mg PSA 100/pk KS0-9507
1200 mg MgSO4, 400 mg PSA, 400 mg C18E, 400 mg GCB 100/pk KS0-9518
1200 mg MgSO4, 400 mg PSA, 400 mg C18E 100/pk KS0-9516
1200 mg MgSO4, 400 mg PSA, 400 mg GCB 100/pk KS0-9517
1200 mg MgSO4, 400 mg PSA 100/pk KS0-9515

| ROQ | QUECHERS KITS

roQ Extraction Salt Packets
Salt packets only. Centrifuge tubes not included.
www.phenomenex.com/roQ
Ordering Information
Description Unit Part No.
AOAC 2007.01 Method Extraction Packets
• Applications
6.0 g MgSO4, 1.5 g NaOAc 50/pk AH0-9043 • Technical Notes
EN 15662 Method Extraction Packets • Tutorials and Webinars
4.0 g MgSO4, 1.0 g NaCl, 1.0 g SCTD, 0.5 g SCDS 50/pk AH0-9041 • Tools
Original Non-Buffered Method Extraction Packets
• And More
4.0 g MgSO4, 1.0 g NaCl 50/pk AH0-9042
6.0 g MgSO4, 1.5 g NaCl 50/pk AH0-9044

Bulk roQ QuEChERS Sorbents

Ordering Information
Phase 10 g 100 g
C18-E — 04G-4348
GCB (Graphitized Carbon Black) 04D-4615 04G-4615
PSA — 04G-4610

We’re here to help!

Speak with your Sample Preparation Specialist

For Additional Food Resources Visit:

www.phenomenex.com/food

Phenomenex 85
 Sample Preparation Tools and Resources

Syringe Filter
Finder Tool
Search Hundreds of
Applications
SAMPLE PREPARATION RESOURCES | SAMPLE PREPARATION

Visit: www.phenomenex.com/SamplePrep

Sample Preparation
Support at Your
Fingertips

SPE Method
Development Tool

Sample Preparation
Basics Overview

86 Phenomenex
 GC

87 - 185

GC Column Selection Guidelines

The Master Resolution Equation.............................................. 88
Choosing Your Selectivity....................................................88-90
Choosing Your Dimensions.................................................91-92

“
Column Selection Charts.....................................................93-99

Zebron GC Columns  The chromatography quality and

performance are excellent [with Zebron].
Meet Your GC Column Family................................................ 101
Column bleed is minimal at 320 °C. Peak
Zebron Unlimited.............................................................102-131
quality remains good for 5 to 6 months
Zebron plus......................................................................132-143

”
averaging 40 injections in a 24 hour
Zebron Inferno™...............................................................144-151 period, 6 to 7 days per week.
Zebron Essentials............................................................152-162
Zebron Guard Columns...................................................163-164
Kevin Walkup
Specialized Assays, Inc.
GC Accessories The opinions stated herein are solely those of the speaker
and not necessarily those of any company or organization.
Inlet Supplies...................................................................165-180
Column Unions & Splitters..................................................... 181
Gas Management.............................................................182-183
Gas & Moisture Traps............................................................. 184
Tools & Maintenance Kits....................................................... 184
GC Column Check Mixes....................................................... 185
Phenomenex 87
 The Master Resolution Choosing Your
Equation Selectivity
The Basic Principle of Column Selectivity Has the Biggest
Selection Impact on Resolution
How do you choose a column? Do you reach into a cabinet of Resolution between two analytes is mainly determined by the se-
mystery columns, look to your favorite 5 % phenyl phase, or bor- lectivity of the stationary phase. By increasing the resolution be-
row one from a colleague? Understanding how column paramaters tween two compounds, the total analysis time can often be reduced
impact key elements of the master resolution equation will help you significantly!
quickly make the right column selection for successful separations.

Selectivity vs. Polarity

Polarity gives a general guideline for sample capacity and sepa-
ration, which can affect peak shape and resolution. However, two
columns may have similar polarity but show different separation
Efficiency Term Selectivity Term Retention Term
profiles due to dissimiliar phase chemistries. For example, ZB-35
and ZB-1701 are close in polarity, but the cyanopropyl group makes
Relates to: Column Length Column Phase Column ID
ZB-1701 very different from ZB-35 in terms of selectivity.
Column ID Film Thickness

Other Carrier Gas Temperature Temperature

Considerations: Linear Velocity

ZB-35
Polarity: 18

CH 3
Si O Si O

CH 3
GC COLUMN SELECTION GUIDELINES | GC COLUMNS & ACCESSORIES

65 % 35 %
Dimethylpolysiloxane Phenyl

Phase Film Thickness

ZB-1701
Polarity: 19
C N
CH 3 (CH 2 )3
Polyimide Coating
Si O Si O
Fused Silica Glass
CH 3

86 % 14 %
Dimethylpolysiloxane Cyanopropylphenyl

88 Phenomenex
Choosing Your Selectivity (cont’d)
The 3 Most Prevalent GC Interactions
The following selection guidelines can be a starting point for choos-
ing Zebron™ columns in common selectivities. Please contact your
Phenomenex representative for additional assistance.

Dispersive Forces (Van der Waals Interactions)

• Weakest of all intermolecular forces and occurs between non-polar
compounds
• Separation is based on boiling point (classic example – hydrocarbon
separation in SimDist analysis)

Dipole-Dipole Interactions

• Either permanently present or induced by analyte-stationary phase

interactions
• Higher dipole-dipole interaction can help separate compounds with similar
boiling points, but different chemical structures

Hydrogen Bonding (Acid-Base Interactions)

• Can cause poor peak shape or irreversible binding to the inlet liner or to the
column itself
• Zebron columns are specially deactivated to minimize these interactions

Choosing A “5” Phase

GC COLUMNS & ACCESSORIES | GC COLUMN SELECTION GUIDELINES

Active analytes or
Your Sample Dirty matrices Standard samples and demanding
Contains or active compounds wide range of analytes method specifications

Versatility for Improved peak

Your High Versatility
0.53 mm ID
multiple shapes, responses,
Inertness and
or thick film versatility for
Analysis temperature for multiple
(> 0.50 µm)
analysis types or sensitivity
biological, chemical,
stability analysis types or reduced for environmental
Requires dimensions
bleed matrices
or food matrices

ZB-5HT ZB-
Phase Inferno™ ZB-5PLUS™ ZB-5 ZB-5ms SemiVolatiles ZB-5MSPLUS
™

5% 5% 5% 5% 5% 5%
Phenyl Phenyl Phenyl Phenyl-Arylene Phenyl-Arylene Phenyl-Arylene
Stable to 430 °C Highly inert, Extremely Extremely versatile; Enviro-Inert™ phase Highly inert,
for extended low-bleed versatile; good good starting point recommended for versatile phase for
lifetime and high phase starting point for method SVOCs, PBDEs, chemical,
temp bakeouts recommended for method developers or other active biological, or
for drug screens developers with better selectivity compounds food matrices
and for aromatics
active analytes

CH3 CH 3 CH 3
ZB-5HT Inferno, ZB-5, and ZB-5PLUS are ZB-SemiVolatiles, ZB-5MSPLUS, and
Si O 5 % phenyl based phases and are versatile Si Si O ZB-5ms are 5 % phenyl-arylene based
for a wide analyte range. phases and provide better separation
of aromatics.
CH 3 CH 3 CH 3

Phenomenex 89
 Choosing Your Selectivity (cont’d)
Choosing A “1” Phase

Your Sample
Non-polar analytes to be separated by true boiling point elution
Contains

Your
High temperature Improved signal-to-noise
Analysis column diameters ratios for better GC-MS
General purpose
performance
Requires < 0.53 mm sensitivity

Phase ZB-1HT Inferno™ ZB-1PLUS™ ZB-1

100 % Dimethylpolysiloxane 100 % Dimethylpolysiloxane 100 % Dimethylpolysiloxane

Stable to 430 °C for extended Extremely versatile with Extremely versatile – good
lifetime and high temp reduced bleed for mass starting point for boiling point
bakeouts spectrometer analysis elutions
GC COLUMN SELECTION GUIDELINES | GC COLUMNS & ACCESSORIES

Choosing A “PEG” Phase

Your Sample FAMEs, Flavors, Essential Oils, Solvents, Free Fatty Acids or
Contains or BTEX Compounds Organic Acids

Your Aqueous stability for Improved signal-to- Efficiency and

Analysis polar compounds; noise ratio for better inertness for acidic
low temperature GC-MS sensitivity analytes
Requires volatiles

Phase ZB-WAX PLUS™ ZB-WAX ZB-FFAP

Polyethylene Glycol Polyethylene Glycol Nitroterephthalic Acid
Modified Polyethylene Glycol
100 % aqueous stability General-purpose Excellent peak shape for
with excellent separations PEG phase for polar underivatized acids,
of polar complex mixtures; food samples organic acids, free fatty acids,
widely used for and alcohols
profiling and "fingerprinting"

90 Phenomenex
Choosing Your Dimensions
Length
Longer columns can improve resolution, but they will also increase
run times. Under isothermal conditions, doubling column length
only increases resolution by 41 %, but doubles the run time! Choose
a column length that balances efficiency with acceptable run times.

Short Long
15 m or less Good Starting Length 60 m or more
Applications Applications
• High boilers • Complex samples with closely eluting
• GC-MS applications 30 m peaks
• Low boilers
• Less active samples
Advantages
• Complex temperature ramps
• Faster run times
• Higher temp. limits
Advantages
• Lower bleed
• Higher efficiency • Better resolution

Disadvantages Disadvantages

• Less inert • Slow run times

• Limited retention

GC COLUMNS & ACCESSORIES | GC COLUMN SELECTION GUIDELINES

Try The GC Column
Finder!
Easily select a column by part number,
manufacturer, industry, application, or official
method in under 1 minute.

www.phenomenex.com/FindGC

Phenomenex 91
 Choosing Your Dimensions (cont’d)
Internal Diameter
Column internal diameter (ID) has a major impact on both resolu-
tion and sample capacity. Unlike column length, using smaller ID
columns can actually lead to faster run times, because the column
length required with a small ID is often shorter due to increased
efficiency.

Narrow Wide
0.10, 0.18, 0.20 mm Good Starting ID 0.32, 0.53 mm
Applications Applications
• Complex samples • Dirty samples
0.25 mm • Highly concentrated samples
Advantages
• Faster run times Advantages
• Better resolution • Increased sample capacity
• Good for on-column injections
Disadvantages
• Lower sample capacity Disadvantages
• Easily overloaded • Decreased efficiency
• May need higher flow rates
• Not compatible with most GC-MS
GC COLUMN SELECTION GUIDELINES | GC COLUMNS & ACCESSORIES

Film Thickness
Film thickness determines solute retention and plays an important
role in column sample capacity. Thin film columns are faster and
provide higher resolution, but lower sample capacity. In most in-
stances, choose the thinnest film possible that still provides ade-
quate retention. When working with active samples, using a slightly
thicker film can significantly improve peak shape.

Thin Thick
0.10, 0.18 µm Good Starting Film 0.50 µm or more
Applications Applications
• High boilers • Low boilers
• GC-MS applications 0.25 µm • Gases, solvents, purgeables, volatiles
• Purity testing
Advantages
• Faster run times Advantages
• Higher temp. limits • Better inertness
• Lower bleed • Higher capacity
• Higher efficiency
Disadvantages
Disadvantages • Slow run times
• Less inert • Lower temp. limits
• Limited retention • Higher bleed

92 Phenomenex
Cross-Reference by Manufacturer
Upgrade to Zebron!
Our commitment to quality and innovation is what makes Zebron GC columns well-suited for any application. Performance is GUARANTEED.

Zebron Phase Zebron Composition Restek® Agilent® Supelco® SGE® OV

Rtx®-1, DB®-1, DB-2887, DB-1 EVDX, SPB®-1, SPB-1 TG, BP1, BP1-PONA,
ZB-1 100 % Dimethylpolysiloxane Rtx-1PONA, HP-1, HP-101, HP-PONA,Ultra 1, SE-30, MET-1, BPX1-SimD OV-1
Rtx-1 F&F CP-Sil 5 CB SPB-1 Sulfur, SPB-HAP

HP-PONA, DB-PETRO,
ZB-DHA-PONA 100% Dimethylpolysiloxane Rtx-DHA Petrocol®-DH
CP-Sil PONA CB

DB-1ms, DB-1ms Ultra Inert,

MDN-1,
ZB-1plus™ 100 % Dimethylpolysiloxane Rtx-1ms, Rxi®-1ms HP-1ms, HP-1ms Ultra Inert, SolGel-1ms™
Equity®-1
CP-Sil 5 CB MS, VF-1ms
ZB-1HT Inferno™ 100 % Dimethylpolysiloxane Rxi-1HT DB-1ht, CP-SimDist Petrocol 2887
MXT®-1HT SimDist, CP-SimDist UltiMetal, CP-Sil 8 CB
ZB-1XT SimDist 100 % Dimethylpolysiloxane MXT-1, MXT-1 SimDist, UltiMetal, BPX1-SimD, DB-PS1,
MXT-2887 DB-HT SimDis, DB-PS2887
DB-5, HP-5, Ultra 2, MDN-5, SPB-5, PTE-5,
5 % Phenyl
ZB-5 Rtx-5 HP-PAS-5, CP-Sil 8 CB, SE-54, PTA-5, Equity-5, BP5, BPX5 OV-5
95 % Dimethylpolysiloxane
HP-5ms Ultra Inert Sac-5
5 % Phenyl Rtx-5ms, Rxi-5ms,
ZB-5plus™ DB-5, HP-5ms, HP-5msi MDN-5S
95 % Dimethylpolysiloxane Rtx-5Amine
5 % Phenyl Rxi-5HT, Rtx-5HT
ZB-5HT Inferno DB-5ht, VF-5ht HT-5
95 % Dimethylpolysiloxane Stx®-5HT, XTI®-5HT
5 % Phenyl-Arylene Rtx-5Sil MS, DB-5ms, DB-5.625, VF-5ms,
ZB-5ms
95 % Dimethylpolysiloxane Rxi-5Sil MS DB-5ms EVDX, CP-Sil 8 CB MS
5 % Phenyl-Arylene DB-5ms Ultra Inert, HP-5ms Ultra
ZB-5MSplus™ Rxi-5Sil MS SLB®-5ms
95 % Dimethylpolysiloxane Inert, DB-5ms, VF-5ms
5 % Phenyl-Arylene Rxi-5Sil MS,
ZB-SemiVolatiles DB-5ms Ultra Inert SLB-5ms
95 % Dimethylpolysiloxane Rxi-5ms
35 % Phenyl Rtx-35, DB-35, DB-35ms, MDN-35, SPB-35,
ZB-35 BPX35, BPX608 OV-11
65 % Dimethylpolysiloxane Rtx-35ms HP-35, HP-35ms SPB-608
35 % Phenyl
ZB-35HT Inferno
65 % Dimethylpolysiloxane Phenomenex Exclusive
50 % Phenyl DB-17, DB-17HT, DB-17ms, HP-50+,
ZB-50 Rtx-50 SP-2250, SPB-17, SPB-50 BPX50 OV-17
50 % Dimethylpolysiloxane DB-17 EVDX, CP-Sil 24 CB

GC COLUMNS & ACCESSORIES | GC COLUMN SELECTION GUIDELINES

6  % Cyanopropyl­phenyl Rtx-1301, DB-1301, DB-624, DB-VRX, HP-VOC,
ZB-624 SPB-1301, SPB-624 BP624 OV-624
94 % Dimethylpolysiloxane Rtx-624 CP-1301, CP-Select 624 CB

CP-Select 624 CB,

ZB-624plus™ Proprietary Rxi-624Sil MS
DB-624UI Ultra Inert
14  % Cyanopropyl­phenyl
ZB-1701 Rtx-1701 DB-1701, CP-Sil 19 CB SPB-1701, Equity-1701 BP10 OV-1701
 86 % Dimethylpolysiloxane
14  % Cyanopropyl­phenyl
ZB-1701P DB-1701P
 86 % Dimethylpolysiloxane
ZB-FAME High Cyanopropyl CP-Sil 88, HP-88, DB-23 SP®-2560, SP-2380
Rtx-WAX,
DB-WAXetr, HP-INNOWax,
ZB-WAX Polyethylene Glycol Famewax, MET-Wax, Omegawax SolGel-WAX™
CP-Wax 57 CB
Stabilwax-DB
DB-WAX, CAM, HP-20M,
ZB-WAXplus™ Polyethylene Glycol Stabilwax® SUPELCOWAX® 10 BP20 Carbowax 20M
Carbowax 20M, CP-Wax 52 CB
Nitroterephthalic Acid Modified DB-FFAP, HP-FFAP, CP-FFAP CB
ZB-FFAP Stabilwax-DA Nukol, SPB-1000 BP21 OV-351
Polyethylene Glycol CP-Wax 58 FFAP CB
Rtx-CLPesticides,
ZB-MultiResidue™-1 Proprietary
Stx-CLPesticides
Rtx-CLPesticides2,
ZB-MultiResidue-2 Proprietary
Stx-CLPesticides2
Rtx-CLPesticides,
ZB-CLPesticides-1 Proprietary
Stx-CLPesticides
Rtx-CLPesticides2,
ZB-CLPesticides-2 Proprietary
Stx-CLPesticides2
ZB-XLB Proprietary Rtx-XLB, Rxi-XLB DB-XLB, VF-XMS MDN-12
ZB-XLB-HT Inferno Proprietary Phenomenex Exclusive
ZB-Drug-1 Proprietary Phenomenex Exclusive
ZB-BAC-1 Proprietary Rtx-BAC1 DB-ALC1
ZB-BAC-2 Proprietary Rtx-BAC2 DB-ALC2
ZB-Bioethanol Proprietary Phenomenex Exclusive
ZB-PAH-EU Proprietary Rxi-PAH DB-PAH-EU
ZB-PAH-CT Proprietary PAH-Select
ZB-Dioxin Proprietary Rtx-Dioxin-2 DB-Dioxin, DB-225, DB-5MSUI SP-2330
This section is, neither in terms of manufacturers nor in terms of their products, a complete list, and the accuracy of the data is not guaranteed.
Small differences in dimensions or performance might be possible and slight adjustments to your application may be necessary.

Phenomenex 93
 Environmental Selection Chart
Listed below are recommended Zebron columns for environmental and EPA methods. Other columns may also be used for these analyses
— please contact Phenomenex for your specific GC column needs.
Drinking Water Method # Description Primary Column Confirmation Column Page
501.3 Trihalomethanes by GC-MS with Selected Ion Monitoring (SIM) ZB-624, ZB-624plus™ 157, 140
502.2 Volatile Halogenated Organics by Purge & Trap GC/PID/ELCD ZB-624, ZB-624plus 157, 140
503.1 Volatile Aromatics and Unsaturated Organics by Purge & Trap GC ZB-624, ZB-624plus 157, 140
504.1 1,2-Dibromoethane (EDB), 1,2-Dibromo-3-chloropropane (DBCP), and ZB-CLPesticides-1 ZB-CLPesticides-2 116
1,2,3-Trichloropropane (123TCP) by GC ZB-MultiResidue™-1 ZB-MultiResidue-2 118
505 Organohalide Pesticides & Aroclors by GC-ECD ZB-CLPesticides-1 ZB-CLPesticides-2 116
ZB-MultiResidue-1 ZB-MultiResidue-2 118
507 Nitrogen & Phosphorus Containing Pesticides by GC-NPD ZB-MultiResidue-1 ZB-MultiResidue-2 118
ZB-CLPesticides-2 ZB-CLPesticides-2 116
508 Chlorinated Pesticides by GC-ECD ZB-CLPesticides-1 ZB-CLPesticides-2 116
ZB-MultiResidue-1 ZB-MultiResidue-2 118
509 Ethylene Thiourea (ETU) by GC-NPD ZB-WAXplus™ ZB-1701 138, 158
513 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin by GC-HRMS ZB-SemiVolatiles 114
515.3 Chlorinated Acids by Liquid-Liquid Extraction, Derivatization and GC-ECD ZB-XLB ZB-35 162, 155
521 Nitrosamines by Solid Phase Extraction (SPE) and GC-MS/MS with Large ZB-SemiVolatiles 114
Volume Injection
522 1,4-Dioxane by Solid Phase Extraction (SPE) and GC-MS with Selected Ion ZB-SemiVolatiles 114
Monitoring (SIM)
523 Triazine Pesticides and their Degradates by GC-MS ZB-50 156
524.3 Purgeable Organic Compounds by GC-MS ZB-624, ZB-624plus 157, 140
525.2 Semi-volatile Organic Chemicals by Solid Phase Extraction (SPE) and GC-MS ZB-SemiVolatiles 114
526 Selected Semi-volatile Organic Compounds by Solid Phase Extraction (SPE) ZB-SemiVolatiles 114
and GC-MS
527 Selected Pesticides and Flame Retardants by Solid Phase Extraction (SPE) ZB-5plus™ 134
and GC-MS
528 Phenols by Solid Phase Extraction (SPE) and GC-MS ZB-SemiVolatiles ZB-35 114, 155
529 Explosives and Related Compounds by Solid Phase Extraction (SPE) and GC-MS ZB-5plus™ 134
548 Endothall by Aqueous Derivatization, Liquid-Solid Extraction, and GC-ECD ZB-SemiVolatiles ZB-35 114, 155
551.1 Chlorinated Solvents & Disinfection Byproducts by Liquid-Liquid Extraction ZB-35 155
and GC-ECD
552.3 Haloacetic Acids and Dalapon by Liquid-Liquid Extraction, Derivatization, ZB-CLPesticides-1 ZB-CLPesticides-2 116, 116
and GC-ECD ZB-XLB ZB-35 162, 155
556 Carbonyl Compounds by Pentafluorobenzylhydroxylamine Derivatization and ZB-SemiVolatiles ZB-1701 114, 158
GC-ECD
GC COLUMN SELECTION GUIDELINES | GC COLUMNS & ACCESSORIES

Waste Water Method # Description Primary Column Confirmation Column Page

601 Purgeable Halocarbons by Purge & Trap GC ZB-624, ZB-624plus 157, 140
602 Purgeable Aromatics by Purge & Trap GC ZB-624, ZB-624plus 157, 140
603 Acrolein & Acrylonitrile Purge & Trap GC ZB-624, ZB-624plus 157, 140
604 Phenols by GC-ECD ZB-SemiVolatiles 114
606 Phthalate Esters by GC-ECD ZB-5plus™ 134
607 Nitrosamines by GC-NPD ZB-SemiVolatiles 114
608 Organochlorine Pesticides and PCBs by GC-ECD ZB-MultiResidue-1 ZB-MultiResidue-2 118, 118
609 Nitroaromatics & Isophorone by GC-FID and GC-ECD ZB-SemiVolatiles 114
610 Polynuclear Aromatic Hydrocarbons by GC-FID ZB-PAH-EU 106
ZB-PAH-CT 110
611 Haloethers by GC-ECD ZB-SemiVolatiles ZB-SemiVolatiles 114
612 Chlorinated Hydrocarbons by GC-ECD ZB-SemiVolatiles 114
613 2,3,7,8-Tetrachlorodibenzo-p-dioxin by GC-MS ZB-SemiVolatiles 114
615 Chlorinated Herbicides by GC-ECD ZB-CLPesticides-1 ZB-CLPesticides-2 116, 116
ZB-XLB ZB-35 162, 155
619 Triazine Herbicides by GC-MS ZB-50 156
622 Organophosphorus Pesticides by GC-MS ZB-MultiResidue-1 118
624 Purgeable Volatiles by Purge & Trap GC-MS ZB-624 157
625 Base/Neutral and Acids by GC-MS ZB-SemiVolatiles 114
1613 Tetra- through Octa-Chlorinated Dioxins & Furans by Isotope Dilution
ZB-Dioxin ZB-SemiVolatiles 102, 114
HRGC/HRMS
1614 Polybrominated Diphenyl Esters (PBDEs) by HRGC/HRMS ZB-5HT Inferno™ 146
ZB-SemiVolatiles 114
1618 Organohalide Pesticides, Organophosphorus Pesticides, and Phenoxy-Acid ZB-MultiResidue-1 ZB-MultiResidue-2 118, 118
Herbicides by GC
1624 Volatile Organic Compounds by Isotope Dilution GC-MS ZB-624, ZB-624plus 157, 140
1625 Semi-volatile Organic Compounds by Isotope Dilution GC-MS ZB-SemiVolatiles 114
1653 Chlorinated Phenols by In-Situ Acetylation and GC-MS ZB-SemiVolatiles 114
1657 Organophosphorous Pesticides by GC-FPD ZB-MultiResidue-1 ZB-MultiResidue-2 118, 118
1658 Phenoxy-Acid Herbicides by GC-ECD ZB-MultiResidue-1 ZB-MultiResidue-2 118, 118
1659 Dazomet by GC-NPD ZB-MultiResidue-1 ZB-MultiResidue-2 118, 118
1666 Pharmaceutical Volatile Organic Compounds by Purge & Trap GC or Isotope ZB-SemiVolatiles (Direct Injection) 114
Dilution GC-MS ZB-624 (Purge & Trap), 157
ZB-624plus 140
1668 Polychlorinated Biphenyl (PCB) Congeners by HRGC/HRMS ZB-MultiResidue-1 ZB-1 118, 152
1671 Pharmaceutical Manufacturing Volatile Organic Compounds by GC-FID ZB-1 152
7850 White Phosphorus (P4) by Solvent Extraction and GC-NPD ZB-1 152

94 Phenomenex
Environmental Selection Chart
Listed below are recommended Zebron columns for environmental and EPA methods. Other columns may also be used for these analyses
— please contact Phenomenex for your specific GC column needs.
Solid Waste Method # Description Primary Column Confirmation Column Page
8010B Halogenated Volatile Organics by GC-ELCD ZB-624, ZB-624plus™ 157, 140
8015C Nonhalogenated Organics by GC ZB-5HT Inferno™ 146
8020A Aromatic Volatile Organics by GC-PID ZB-WAX, 160
ZB-WAXplus™ 138
8021B Aromatic and Halogenated Volatiles by GC-PID or GC-ELCD ZB-624, ZB-624plus ZB-1 (thick phase) 157, 140, 152
8030A Acrolein and Acrylonitrile by GC-FID ZB-624, ZB-624plus 157, 140
8032A Acrylamide by GC-ECD ZB-5HT Inferno 146
8041 Phenols by GC-ECD or GC-FID ZB-SemiVolatiles 114
8061A Phthalate Esters by GC-ECD ZB-SemiVolatiles ZB-1701 114, 158
8081B Organochlorine Pesticides by GC-ECD ZB-MultiResidue™-1 ZB-MultiResidue-2 118
ZB-CLPesticides-1 ZB-CLPesticides-2 116
8082A Polychlorinated Biphenyls (PCBs) by GC-ECD ZB-MultiResidue-1 ZB-MultiResidue-2 118
ZB-CLPesticides-1 ZB-CLPesticides-2 116
8091 Nitroaromatics and Cyclic Ketones by GC-ECD or GC-NPD ZB-SemiVolatiles ZB-1701 114, 158
8095 Explosives by GC-ECD ZB-50 156
8100 Polynuclear Aromatic Hydrocarbons by GC-FID ZB-SemiVolatiles 114
ZB-35 155
8121 Chlorinated Hydrocarbons by GC-ECD ZB-MultiResidue-1 ZB-MultiResidue-2 118
8131 Aniline and Selected Derivatives by GC-NPD ZB-SemiVolatiles ZB-1 114, 152
8141B Organophosphorus Pesticides by GC-FPD or GC-NPD ZB-MultiResidue-1 ZB-MultiResidue-2 118
ZB-CLPesticides-1 ZB-CLPesticides-2 116
8151A Chlorinated Herbicides by GC-ECD ZB-CLPesticides-1 ZB-CLPesticides-2 116, 116
ZB-XLB ZB-35 162, 155
8260B Volatile Organic Compounds by GC-MS ZB-624, ZB-624plus 157, 140
8270D Semi-volatile Organic Compounds by GC-MS ZB-SemiVolatiles 114
8272 Polynuclear Aromatic Hydrocarbons (PAHs ) by SPME and GC-MS with ZB-SemiVolatiles, 114

GC COLUMNS & ACCESSORIES | GC COLUMN SELECTION GUIDELINES

Selected Ion Monitoring (SIM) ZB-35 155
8280B Polychlorinated Dibenzo-P-Dioxins (PCDDs) and Polychlorinated Dibenzofurans ZB-SemiVolatiles 114
(PCDFs) By HRGC-LRMS
8290A Polychlorinated Dibenzo-P-Dioxins (PCDDs) and ZB-SemiVolatiles 114
Polychlorinated Dibenzofurans (PCDFs) By HRGC/HRMS
8410 Semi-Volatile Organic Compounds by GC-FTIR ZB-SemiVolatiles 114
8430 Bis(2-chloroethyl) Ether and Hydrolysis Products by ZB-WAXplus 138
Direct Aqueous Injection GC-FTIR

Air Method # Description Primary Column Page

TO-1 Volatile Organic Compounds by Thermal Adsorption and GC-MS ZB-1plus™ 132
TO-2 Volatile Organic Compounds by Carbon Molecular Sieve Adsorption and GC-MS ZB-1plus 132
TO-3 Volatile Organic Compounds by Cryogenic Preconcentration Techniques and ZB-1plus 132
GC-FID /ECD
TO-4A Pesticides and Polychlorinated Biphenyls (PCBs) by ZB-MultiResidue-1 118
High Volume Polyurethane Foam (PUF) Sampling and GC
TO-7 N-Nitrosodimethylamine by GC-MS ZB-WAXplus 138
TO-9A Polychlorinated, Polybrominated, and Brominated/Chlorinated Dibenzo-p-Dioxins and ZB-SemiVolatiles 114
Dibenzofurans by HRGC/HRMS
TO-10A Pesticides and Polychlorinated Biphenyls (PCBs) by ZB-MultiResidue-1 118
Low Volume Polyurethane Foam (PUF) Sampling and GC
TO-13A Polycyclic Aromatic Hydrocarbons (PAHs) by GC-MS ZB-SemiVolatiles 114
TO-14A Volatile Organic Compounds by Specially Prepared Canisters and GC ZB-1plus 132
TO-15 Volatile Organic Compounds by Specially Prepared Canisters and GC-MS ZB-1plus 132

Phenomenex 95
 Food & Flavors Selection Chart
Listed below are recommended Zebron columns for food safety, food quality, and flavor/fragrance methods. Other columns may also be
used for these analyses — please contact Phenomenex for your specific GC column needs.

Food Safety Compound Class Analysis Recommended Columns Page

Pesticides & Antimicrobials Multi-Residue Pesticide Screening ZB-MultiResidue™-1 and -2 118
Organochlorine Pesticides in Water ZB-MultiResidue-1 and -2 118
Organochlorine Pesticides in Foods of Plant Origin ZB-MultiResidue-1 and -2 118
Organophosphorus Pesticides in Foods of Plant Origin ZB-MultiResidue-1 and -2 118
Triazine Pesticides in Water ZB-50 156
Triazine Pesticides in Foods of Plant Origin ZB-50 156
Chloramphenicol in Foods of Animal Origin ZB-1plus™ 132
Environmental Contaminants Polybrominated Diphenyl Ethers (PBDEs) in Food ZB-5MSplus™, ZB-SemiVolatiles, ZB-35 136, 114, 155
Polychlorinated Biphenyls (PCBs) in Water ZB-MultiResidue-1, ZB-XLB-HT Inferno™ 118, 150
Polychlorinated Dibenzo-dioxins (PCDDs) in Food ZB-5MSplus, ZB-SemiVolatiles 136, 114
Polychlorinated Dibenzo-furans (PCDFs) in Food ZB-5MSplus, ZB-SemiVolatiles 136, 114
Polycyclic Aromatic Hydrocarbons (PAHs) in Water ZB-PAH-EU, ZB-PAH-CT, ZB-5MSplus, 106,110,136,
ZB-SemiVolatiles, ZB-35 114, 155
Food Contact Materials Food Packaging Volatiles ZB-624, ZB-624plus™ 157, 140
Dioxins and Furans in Food ZB-Dioxin 102
Melamine in Food ZB-XLB-HT Inferno 150
Cyanuric Acid in Food ZB-XLB-HT Inferno 150
Phthalates in Food ZB-5MSplus 136
Residual Solvents in Food ZB-624, ZB-624plus, ZB-WAXplus 157, 140, 138
Bisphenol A & F (BPA/BPF) in Food ZB-5MSplus 136
Additives & Preservatives Parabens in Food ZB-5MSplus 136
Chloropropanols (3-MCPD) in Food ZB-5MSplus 136
Flavor Additives (Borneol) ZB-MultiResidue-1 118
Phenolic Antioxidants (BHA & BHT) in Food ZB-50 156
GC COLUMN SELECTION GUIDELINES | GC COLUMNS & ACCESSORIES

Tocopherols in Food ZB-5MSplus 136

Process Contaminants Acrylamide in Foods ZB-5HT Inferno 146
Acrylamide, Acrylonitrile, and Acrolein in Water ZB-624, ZB-624plus 157, 140
Benzene in Food ZB-WAXplus 138
Glycols in Food ZB-WAXplus 138
Hormones Steroid Hormones in Food ZB-5MSplus, ZB-1plus 136, 132

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96 Phenomenex
Food & Flavors Selection Chart
Listed below are recommended Zebron columns for food safety, food quality, and flavor/fragrance methods. Other columns may also be
used for these analyses — please contact Phenomenex for your specific GC column needs.
Food Quality Compound Class Analysis Recommended Columns Page
Fatty Acids & FAMEs Food Industry Fatty Acid Methyl Esters (FAMEs) ZB-FAME 112
Marine Oil Fatty Acid Methyl Esters (FAMEs) ZB-FAME 112
Saw Palmetto Fatty Acid Methyl Esters (FAMEs) ZB-FAME 112
Free Fatty Acids ZB-FFAP 161
Essential Fatty Acids (EFAs) Omega-3 and Omega-6 ZB-FAME 112
Triglycerides Butter, Canola Oil, Olive Oil, and Peanut Oil Triglycerides ZB-5HT Inferno™ 146
Alcoholic Beverages Cognac Compounds ZB-WAXplus™ 138
Distilled Liquor Screen ZB-FFAP 161
Ethanol in Beer ZB-Bioethanol 122
Sulfur in Beer ZB-1plus™ 132
Whiskey Compounds ZB-WAXplus 138
Wine Compounds ZB-WAX, ZB-WAXplus 160, 138
Other Acids Organic Acids ZB-FFAP 160
Amino Acids ZB-50 156
Sterols Sterols in Lard, Margarine, Peanut Butter, or Olive Oil ZB-5HT Inferno 146
Sugars Alditol Acetates ZB-5MSplus™ 136
Trimethylsilyl (TMS) Sugars ZB-MultiResidue™-1 118

Flavors & Fragrances Compound Class Analysis Recommended Columns Page

Essential Oils Cold-Pressed Orange Oil ZB-WAXplus 138
Ginkgo Biloba Oil, Lavender Oil, and Ylang Ylang Oil ZB-1plus 132
Peppermint Oil ZB-WAX 160

GC COLUMNS & ACCESSORIES | GC COLUMN SELECTION GUIDELINES

Rose Oil ZB-XLB 162
Spearmint Oil ZB-5MSplus 136
Flavors Flavors Screening ZB-FFAP 161
Flavor Allergens ZB-5MSplus 136
Flavor Volatiles ZB-1plus, ZB-WAXplus, ZB-624 132, 138, 157
Alcoholic Beverage Profile ZB-FFAP 161
Honey Profile ZB-WAXplus 138
Fragrances Fragrance Screening ZB-WAXplus, ZB-624 138, 157
Fragrance Allergens ZB-1plus 132

Phenomenex 97
 Pharmaceutical Selection Chart
Listed below are recommended Zebron columns for USP and pharmaceutical methods. Other columns may also be used for these analyses
— please contact Phenomenex for your specific GC column needs.

USP Phase Composition Recommended Columns Page

G1 Dimethylpolysiloxane Oil ZB-1, ZB-1plus™, ZB-1HT Inferno™ 152, 132, 144
G2 Dimethylpolysiloxane Gum ZB-1, ZB-1plus, ZB-1HT Inferno 152, 132, 144
G3 50 % Phenyl 50 % Methylpolysiloxane ZB-50 156
G5 Not less than 70 % of 3-Cyanopropylpolysiloxane ZB-FAME 112
G8 80 % Bis (3-Cyanopropyl-20 % 3-Cyanopropylphenylpolysiloxane) ZB-FAME 112
G9 Methylvinylpolysiloxane ZB-1plus, ZB-1HT Inferno, ZB-1 132, 144, 152
G14 Polyethylene Glycol (Average MW 950-1,050) ZB-WAX, ZB-WAXplus™ 160, 138
G15 Polyethylene Glycol (Average MW 3,000-3,700) ZB-WAX, ZB-WAXplus 160, 138
G16 Polyethylene Glycol (Average MW 15,000) ZB-WAX, ZB-WAXplus 160, 138
G17 75 % Phenyl 25 % Methylpolysiloxane ZB-50 156
G20 Polyethylene Glycol (Average MW of 380-420) ZB-WAX, ZB-WAXplus 160, 138
G25 Polyethylene Glycol TPA (Carbowax 20M Terephthalic Acid) ZB-FFAP 161
G27 5 % Phenyl 95 % Methylpolysiloxane ZB-5, ZB-5plus™, ZB-5HT Inferno 153, 134, 146
5 % Phenyl-Arylene 95 % Methylpolysiloxane ZB-5ms, ZB-5MSplus™, ZB-SemiVolatiles 154, 136, 114
G28 25 % Phenyl 75 % Methylpolysiloxane ZB-35, ZB-35HT Inferno 155, 148
G32 20 % Phenylmethyl 80 % Dimethylpolysiloxane ZB-35, ZB-35HT Inferno 155, 148
G35 Polyethylene Glycol & Diepoxide Esterified with Nitroterephthalic Acid ZB-FFAP 161
G36 1 % Vinyl 5 % Phenylmethylpolysiloxane ZB-5, ZB-5plus, ZB-5HT Inferno 153, 134, 146
G38 Phase G1 Plus A Tailing Inhibitor ZB-1, ZB-1plus, ZB-1HT Inferno 152, 132, 144
G39 Polyethylene Glycol (Average MW 1,500) ZB-WAX, ZB-WAXplus™ 160, 138
G41 Phenylmethyldimethylsilicone (10 % Phenyl Substituted) ZB-5, ZB-5plus, ZB-5HT Inferno 153, 134, 146
G42 35 % Phenyl 65 % Dimethylpolysiloxane ZB-35, ZB-35HT Inferno 155, 148
G43 6 % Cyanopropylphenyl 94 % Dimethylpolysiloxane ZB-624, ZB-624plus™ 157, 140
G46 14 % Cyanopropylphenyl 86 % Methylpolysiloxane ZB-1701, ZB-1701P 158, 159
GC COLUMN SELECTION GUIDELINES | GC COLUMNS & ACCESSORIES

G47 Polyethylene glycol (average MW 8,000) ZB-WAXplus , ZB-WAX 138, 160

G48 Highly polar, partially cross-linked cyanopolysiloxane ZB-FAME 112
G51 50% Phenyl - 50% Dimethylpolysiloxane phase which is modified with ZB-PAH-CT, ZB-PAH-EU 110,106
an aromatic selector for optimized separation of polycyclic aromatic
hydrocarbons (PAH)

Residual Solvents USP <467> Procedure USP Phase for Residual Solvents Recommended Columns Page
Procedure A G43 (6 % Cyanopropyl 94 % Dimethylpolysiloxane) ZB-624, ZB-624plus 157, 140
Procedure B G16 (Polyethylene Glycol) ZB-WAXplus 138
Procedure C G43 or G16 ZB-624plus or ZB-WAXplus 140, 138

Doing Headspace Testing?

Find the right headspace vial for your analysis and learn more about Verex™ Certified Vial Products with our interactive web tool.
www.phenomenex.com/verex

98 Phenomenex
ASTM Method Selection Chart
Listed below are recommended Zebron columns for ASTM methods. Other columns may also be used for these analyses — please contact
Phenomenex for your specific GC column needs.

ASTM Method Description Recommended Columns Page

D 1946 Reformed gas ZB-1 152
D 2268 Analysis of n-heptane and iso-octane (high purity) ZB-1 152
D 2306-96 Xylene isomers ZB-WAX, ZB-WAXplus™ 160, 138
D 2426 Butadiene and styrene in butadiene concentrates ZB-1 152
D 2504 Non-condensable gases in C1-C3 hydrocarbons ZB-1 (thick phase) 152
D 2580 Phenols in water ZB-WAXplus 138
D 2600 Aromatic traces in light saturated hydrocarbons ZB-WAX 160
D 2804 Purity of methyl ethyl ketone ZB-WAX 160
D 2887 SimDist analysis of petroleum fractions ZB-1, ZB-1XT SimDist 152, 124
D 2908 Volatile organics in water ZB-WAX, ZB-WAXplus 160, 138
D 2998 Polyhydric alcohols in alkyd resins ZB-1 152
D 2999 Monopentaerythritol in commercial pentaerythritol ZB-1 152
D 3009 Composition of turpentine ZB-WAXplus 138
D 3054 Purity and benzene content of cyclohexane ZB-1 152
D 3086 Organochlorine pesticides in water ZB-CLPesticides-1 or -2, ZB-MultiResidue™-1 or -2 116, 118
D 3168 Polymers in emulsion paints ZB-1 152
D 3271 Solvent analysis in paints ZB-WAXplus 138
D 3304 PCBs in environmental materials ZB-MultiResidue-1 or -2 118
D 3328 Comparison of waterborne petroleum oils ZB-1 152
D 3329 Purity of methyl isobutyl ketone ZB-WAXplus 138
D 3432 Toluene diisocyanates in urethane prepolymers ZB-1 152
D 3447 Purity of trichlorotrifluoroethane (CFC-113) ZB-1, ZB-624 152, 157
D 3452 Identification of rubber ZB-1HT Inferno™ 144
D 3465 Purity of monomeric plasticizers ZB-1 152
D 3524 Diesel fuel in lubricating oil (SAE 30) ZB-1HT Inferno 144

GC COLUMNS & ACCESSORIES | GC COLUMN SELECTION GUIDELINES

D 3534 PCBs in water ZB-5, ZB-5plus™ 153, 134
D 3606 Benzene and toluene in gasoline ZB-1 152
D 3687 Volatile organic compounds ZB-WAX, ZB-WAXplus 160, 138
D 3710 Gasoline fractions ZB-1XT SimDist 124
D 3725 Fatty acids in drying oils ZB-FFAP 161
D 3760 Analysis of cumene ZB-WAX, ZB-WAXplus 160, 138
D 3797 Analysis of o-xylene ZB-WAX, ZB-WAXplus 160, 138
D 3798 Analysis of p-xylene impurities ZB-WAX, ZB-WAXplus 160, 138
D 3876 Methoxyl and hydroxypropyl substitution in cellulose ether products ZB-1 152
D 3962 Impurities in styrene ZB-FFAP 161
D 4059 PCBs in insulating liquids ZB-5plus, ZB-5HT Inferno 134, 146
D 4275 Butylated hydroxy toluene in ethylene and ethylenevinylacetate polymers ZB-1 152
D 4367 Benzene in hydrocarbon solvent ZB-1 152
D 4420 Aromatics in gasoline ZB-1 152
D 4735 Thiophene impurities in benzene ZB-FFAP 161
D 4768 Phenol and cresol inhibitors in insulating oils ZB-FFAP 161
D 5060 Impurities in ethylbenzene ZB-FFAP, ZB-WAX, ZB-WAXplus 161, 160, 138
D 5134 Petroleum naphthas through n-nonane ZB-1, ZB-DHA-PONA 152, 126
D 5135-95 Analysis of styrene ZB-WAX, ZB-WAXplus 160, 138
D 5441 Analysis of Methyl Tert-Butyl Ether (MTBE) ZB-DHA-PONA 126
D 5501 Determination of denatured bioethanol ZB-1, ZB-Bioethanol, ZB-DHA-PONA 152, 122, 126
D 5580 Aromatics in finished gasoline ZB-1 152
D 6352 Extended SimDist ZB-1HT Inferno, ZB-1XT SimDist 144, 124
D 6584 Determination of glycerine in biodiesel ZB-5HT Inferno 146
D 6729-30, Components in spark ignition fuels ZB-DHA-PONA 126
D 6733
D 7169 Crude Oil; Vacuum distillates ZB-1XT SimDist 124
E 0202 Analysis of glycols ZB-WAXplus, ZB-1 138, 152
E 1100 Analysis of denaturated ethanol ZB-WAXplus, ZB-Bioethanol 138, 122

Phenomenex 99
 ™

GC Columns

Essentials PLUS

A collection of tried-and-true PLUS phases offer a suite of

selectivities, Essentials phases upgrades compared to their
are the smart starting point Essentials counterparts –
for the GC method developer. from exceptional inertness to
enhanced aqueous stability.
ZB-1 ZB-624
ZB-5 ZB-1701 ZB-1plus™
ZB-5ms ZB-1701P ZB-5plus™
ZB-35 ZB-WAX ZB-5MSplus™
ZB-50 ZB-FFAP ZB-WAXplus™
ZB-XLB ZB-624plus™

∞
ZEBRON UNLIMITED GC COLUMNS | GC COLUMNS

Inferno™ Unlimited

Resilient under even the Designed for the truly bold GC scientist,
most intense GC conditions, Unlimited phases unleash the power
Inferno phases dare to defy of selectivity for targeted performance
high boilers, contaminants, that breaks from the mold. 
and carry-overs.
ZB-PAH-EU
ZB-1HT
ZB-PAH-CT
ZB-5HT
ZB-Dioxin
ZB-35HT
ZB-FAME
ZB-XLB-HT
ZB-SemiVolatiles
ZB-MultiResidue™ -1 & -2
ZB-CLPesticides -1 & -2
ZB-Drug-1
ZB-BAC-1 & -2
ZB-1XT SimDist
ZB-Bioethanol
ZB-DHA-PONA
100 Phenomenex
 ™

Meet Your GC Column Family GC Columns

Selected Zebron Polarities

Meet Your GC Column Family
Zebron Unlimited
5 ZB-1 Food Testing
For Non-Polar Analytes
ZB-DHA-PONA ZB-FAME........................................................................ 112
• Alkanes
Environmental Testing
ZB-1plus ™
• Aromatics
ZB-Dioxin........................................................................ 102
ZB-1HT Inferno
™ • Oils
ZB-PAH-EU..................................................................... 106
ZB-1XT SimDist • Boiling Point Separations
ZB-PAH-CT..................................................................... 110
ZB-SemiVolatiles............................................................ 114
8 ZB-5 ZB-CLPesticides-1 & -2................................................. 116
ZB-5ms ZB-MultiResidue™-1 & -2............................................... 118

ZB-5plus™ Fuels
ZB-Dioxin........................................................................ 102
ZB-5MSplus™
ZB-PAH-EU..................................................................... 106
ZB-5HT Inferno
ZB-PAH-CT..................................................................... 110
ZB-SemiVolatiles ZB-Bioethanol................................................................ 122
ZB-1XT SimDist.............................................................. 124
9 ZB-XLB ZB-DHA-PONA............................................................... 126
ZB-XLB-HT Inferno Forensics & Toxicology
ZB-Drug-1....................................................................... 128
ZB-BAC-1 & -2................................................................ 130
11 ZB-MultiResidue™-1
Zebron plus

GC COLUMNS & ACCESSORIES | GC COLUMN SELECTION GUIDELINES

Polarity

ZB-1plus™....................................................................... 132
13 ZB-624 For Slightly Polar Analytes ZB-5plus™....................................................................... 134
ZB-624plus™ • Volatiles ZB-5MSplus™.................................................................. 136
• Drugs ZB-WAXplus™................................................................. 138
15 ZB-MultiResidue-2 • Pesticides ZB-624plus™................................................................... 140
Zebron Inferno™
ZB-1HT Inferno............................................................... 144
18 ZB-35
ZB-5HT Inferno............................................................... 146
ZB-35HT Inferno
ZB-35HT Inferno............................................................. 148
ZB-XLB-HT Inferno........................................................ 150
19 ZB-1701 Zebron Essentials
ZB-1701P ZB-1................................................................................ 152
ZB-5................................................................................ 153
ZB-5ms........................................................................... 154
24 ZB-50
ZB-35.............................................................................. 155

ZB-50.............................................................................. 156
ZB-624............................................................................ 157
52 ZB-WAXplus™ ZB-1701.......................................................................... 158
For Very Polar Analytes
ZB-1701P........................................................................ 159
• Polar Volatiles
57 ZB-WAX ZB-WAX.......................................................................... 160
• Alcohols
ZB-FFAP......................................................................... 161
• Phenols
ZB-XLB........................................................................... 162
58 ZB-FFAP • Acids
Zebron Guard Columns
Guardian™ Integrated Guard Columns.......................... 163
Z-Guard™ Columns......................................................... 164

Phenomenex 101
 Zebron ™
GC Columns

ZB-Dioxin
Improve Lab Productivity by 50% Upgrade to Zebron from traditional phases used for Dioxin analysis:
• Fast PCB analysis Agilent® Restek® Supelco®
• DB®-5MSUI • Rtx®-Dioxin2 • SP®-2330
• Enhanced resolution of TCDD and TCDF • DB-Dioxin
• DB-225
• Improved column lifetime with integrated guard
column option
• MS certified, low bleed GC column

Why Choose ZB-Dioxin?

A Proprietary Phenyl phase which provides

improved resolution of critical dioxin isomers

Intermediate polarity and thin film (60 meter x 0.20 µm x 0.25 mm) to

reduce analysis times and increase productivity

Extensive cross-linking through ESC™

(Engineered Self Cross-Linking™) for low bleed
and high temperature stability to minimize GC-
MS maintenance and system downtime.
ZEBRON UNLIMITED GC COLUMNS | GC COLUMNS

Our Customer Says YES!

ZB-Dioxin for Fast Dioxin and PCB
Analysis
Zebron ZB-Dioxin columns are specifically tailored for the analysis
“ The ZB-Dioxin achieves superior resolution
for both 2,3,7,8-TCDD and 2,3,7,8-TCDF
of aromatic compounds like dioxins, furans and PCBs in Food and
Environmental matrices. The current analysis of Tetra through Octa while not only maintaining chromatography
Dioxin and Furan is time consuming and requires two GC columns for the hexes but actually improving it.
and two GC-HRMS instruments. By switching to the unique selec- This is all performed while reducing the
tivity of ZB-Dioxin analysis, you will gain enhanced resolution of overall runtime over traditional 5ms dioxin
2,3,7,8-TCDD and 2,3,7,8-TCDF from its isomers in one run, and
only need a single ZB-Dioxin GC column. In addition, ZB-Dioxin
columns by as much as 25%. The ZB-Dioxin
serves as your single column solution for Dioxin and PCB analysis. increases throughput by not only eliminating
Upgrade your existing GC column for Dioxin analysis to a Zebron the need for a second column confirmation,
ZB-Dioxin GC column and get all the analytical benefits and pro- but also by allowing additional samples “
ductivity gains of a single column solution. to be analyzed in each 12-hour analytical
Learn more at: sequence.
www.phenomenex.com/GCDioxin
Andrew Patterson,Technical Director
Eurofins Specialty Services, USA
The opinions stated herein are solely those of the speaker and
not necessarily those of any company or organization.

102 Phenomenex
Zebron ™
GC Columns

ZB-Dioxin (cont’d)
Increase Throughput and Improve Resolution of Dioxins and PCBs
by using Zebron ZB-Dioxin!
Zebron ZB-Dioxin has a unique phase that allows for improved resolution of critical dioxin and PCB congeners
and its consistent film thickness allows for optimal dioxin analysis on a single column.

Traditional ZB-Dioxin Upgrade Traditional ZB-Dioxin Upgrade

VS. VS.

• PCB and Dioxin required 2 • Zebron ZB-Dioxin is a single • Higher analysis cost: • Lower analysis cost:
different GC columns column solution for Dioxin 2 GC-HRMS + 2 GC columns 1 GC-HRMS + 1 GC column
and PCB

Traditional ZB-Dioxin Upgrade Traditional ZB-Dioxin Upgrade

GC COLUMNS | ZEBRON UNLIMITED GC COLUMNS

VS. VS.

~90 mins ~40 mins

• Long run time for Dioxin analysis

• First column (5% phenyl phase) • Faster run time using one • Shorter column lifetime for • Longer column lifetime with
~60 minutes ZB-Dioxin ~40 minutes difficult matrix like soil ZB-Dioxin Guardian™ option
• Second column (225 phase) (Part No: 7KG-G045-10-GGA)
~30 minutes

Traditional ZB-Dioxin Upgrade Traditional ZB-Dioxin Upgrade

VS. VS.
~290 °C 320/340 °C

• Lower throughput from customer • HIGH throughput from customer • Some GC Dioxin columns do • Low Bleed GC column temp
perspective perspective not exceed 290 °C Temperature with 320/340 °C Temperature
Limits Limits, this will provide higher
sensitivity for later eluters and
the ability to bake out major
contaminants.

Phenomenex 103
 Zebron ™
GC Columns

ZB-Dioxin (cont’d)
TCDD on a Zebron ZB-Dioxin and a Popular Brand A
Zebron ZB-Dioxin GC Column Brand A Premium 5MS Phase
Part No. 7KG-G045-10 60 meter x 0.25 mm x 0.25 μm
60 meter x 0.25 mm x 0.20 μm

100 3
4 5 100 3
5
1 4
1

2
2
%

%
App ID 26010

App ID 26011
0 0
20 21 22 23 24 25 26 27 28 29 30 31 32 33 min. 20 21 22 23 24 25 26 27 28 29 30 31 32 33 min.

High Resolution of 2,3,7,8-TCDD by using ZB-Dioxin which Run Time (min) 2,3,7,8-TCDD is not completely
exceeds 25 % valley EPA-1613 method requirement and Sample: ZB-Dioxin Brand A resolved which affects the
provided extended lifetime 1. 1,3,6,8-TCDD 25.65 23.20 column lifetime
2. 1,2,3,7-TCDD 30.58 30.33
3. 1,2,3,8-TCDD 30.75 30.55
4. 2,3,7,8-TCDD 31.07 30.78
5. 1,2,8,9-TCDD 32.41 32.13

Conditions for all separations:

Column 1: Zebron ZB-Dioxin Carrier Gas: Helium @ 1.25 mL/min (constant flow)
Column 1 Dimension: 60 meter x 0.25 mm x 0.20 μm Oven Program: 160 °C for 2.4 min to 200 °C @ 25 °C/min to 220 °C @ 5 °C/min for
Column Part No.: 7KG-G045-10 19 min to 288 °C @ 4 °C/min to 300 °C @ 5 °C/min for 7.6 min
Column 2: Brand A Premium 5MS Detector: HRMS
ZEBRON UNLIMITED GC COLUMNS | GC COLUMNS

Column 2 Dimension: 60 meter x 0.25 mm x 0.25 μm Transfer Line Temperature: 300 C

Guard Column: 5 meter Z-Guard™ Kit
Guard Kit Part No.: 7AG-G000-00-GZK
Injection: Pulse Splitless (2.0 min, 60 psi) @ 280 °C, 1 μL
Liner: Zebron PLUS 4 mm ID Single Taper Liner
Liner Part No: AG2-0A10-05

TCDF on a Zebron ZB-Dioxin and a Popular Brand A

Zebron ZB-Dioxin GC Column Brand A Premium 5MS Phase
Part No. 7KG-G045-10 60 meter x 0.25 mm x 0.25 μm
60 meter x 0.25 mm x 0.20 μm
26012
1 100 1
100

5
3 5
2 2 3
4
4
%

%
App ID 26012

App ID 26013

0 0
20 21 22 23 24 25 26 27 28 29 30 31 32 33 min. 20 21 22 23 24 25 26 27 28 29 30 31 32 33 min.

Complete resolution of 2,3,7,8-TCDF on Run Time (min) 2,3,7,8-TCDF are not completely resolved and
a single column ZB-Dioxin– Sample: ZB-Dioxin Brand A need an additional GC column to confirm iso-
NO NEED FOR ADDITIONAL CONFIRMATION COLUMN 1. 1,3,6,8-TCDF 23.20 23.16 mers separation
2. 1,3,4,7-TCDF 29.57 29.23
3. 2,3,7,8-TCDF 29.98 29.53
4. 1,2,3,9-TCDF 31.14 30.76
5. 1,2,8,9-TCDF 32.79 32.33

Comparative separations may not be representative of all applications.

104 Phenomenex
Zebron ™
GC Columns

ZB-Dioxin (cont’d) Column Profile

Fast GC-MS/MS Analysis of PCBs on a Single 40 Meter

Zebron ZB-Dioxin GC Column
POLARITY MS CERTIFIED

PROPRIETARY
1, 2 3 5 7 14, 15 18 20
1 4 8
9
6
13 TEMP LIMITS ( °C )
11 40 to 320/340
0.8

0.6 17
16

12 19
0.4 10

App ID 26563
0.2 Engineered Self Cross-linking™ (ESC)

Phase Chemistry
0
9 10 11 12 13 14 15
Counts (%) vs. Acquisition Time
16 17 18 19 min
• Proprietary

Recommended Applications

GC-MS/MS Conditions: Analyte: 1. PCB-28 • Dioxin and PCB in Food,

Column: Zebron ZB-Dioxin 2. PCB-31 Environmental Samples
Dimension: 40 meter x 0.18 mm x 0.14 μm 3. PCB-52
4. PCB-101 • POPs in Food
Part No.: 7PD-G045-47
Injection: Splitless for 1.5 min @ 290 °C, 1 μL 5. PCB-81
Recommended Liner: Zebron PLUS Z-Liner™ 6. PCB-77
(Compatible with Agilent® & Thermo® GC instrument) 7. PCB-123
Part No.: AG2-0A13-05 8. PCB-118 Zebron GC Columns MS Certification,

GC COLUMNS | ZEBRON UNLIMITED GC COLUMNS

Carrier Gas: Helium @ 0.8 mL/min (constant flow) 9. PCB-114 see p. 437
Oven Program: 45 °C for 0 min to 175 °C @ 50 °C/min, to 220 °C @ 15 °C/min, to 10. PCB-153
250 °C @ 5 °C/min for 3 min, to 300 °C @ 50 °C/min for 10 min 11. PCB-105
Detector: GC-MS/MS 12. PCB-138
Contact Phenomenex or your local
Transfer Line Temperature: 300 °C 13. PCB-126
14. PCB-167
Phenomenex distributor for additional
Mode: Scan (100-450 m/z) GC products and applications.
Source Temperature: 300 °C 15. PCB-128
Quad Temperature: 150 °C 16. PCB-156
Solvent Delay: 8.0 min 17. PCB-157
18. PCB-180 Extend column lifetime.
19. PCB-169 Add a Z-Guard™ to your next
20. PCB-189 Zebron GC order.

Ordering Information
Zebron ZB-Dioxin GC Columns
ID (mm) df (µm) Temp. Limits ºC Part No.
40-Meter
0.18 0.14 40 to 320/340 7PD-G045-47
60-Meter
0.25 0.20 40 to 320/340 7KG-G045-10
60-Meter with 5-Meter Guardian™ Integrated Guard
0.25 0.20 40 to 320/340 7KG-G045-10-GGA

Phenomenex 105
 Zebron ™
GC Columns

ZB-PAH-EU
1.25
6 7 8
• 1.00
Up to 70 % faster PAH analysis Upgrade to Zebron from traditional phases used for PAHs:
• 0.75
Elevated temperature stability (340/360 °C) Agilent® Restek®
• 0.50
Great resolution of critical isomers, • DB®-EUPAH • Rxi®-PAH
0.25
e.g. Benzo[b,j,k]fluoranthene
0

25 25.1 25.2 25.3 25.4 25.5 25.6 25.7 25.8 25.9 26 26.1 26.2
Priority PAH Analysis by GC
Zebron ZB-PAH-EU
7
columns are designed to move conventional
6 8
PAH testing to the exceptional, Zebron GC columns come to life 4
2
through a coupling of innovative spirit and technical excellence.
3

The Zebron ZB-PAH-EU and ZB-PAH-CT offer unparalleled per-

formance through the power of targeted selectivity when analyzing
Polycyclic Aromatic Hydrocarbons (PAHs).

25.1 25.2 25.3 25.4 25.5 25.6 25.7 25.8 25.9 26 26.1 26.2

Analysis of EU 15+117.8
PAHs17.9 18 18.1 18.2 18.3 18.4 18.5 18.6 18.7 18.8 18.9 19 19.1

1.25 4 11
2 6 7 8 10
1.00 3
Excellent Resolution of EU
1.5 PAH Critical Pairs
0.75

1 0.50

0.25
0.5
0
0

17.9 18 18.1 18.2 18.3 29 18.4 18.5 18.6 25 18.7 25.1 18.8
29.5 30 25.2 18.9 25.3 19 25.4
30.5 19.125.5 25.6 31 25.7 25.8 31.5
25.9 26
31.75 26.1
32 26.2
ZEBRON UNLIMITED GC COLUMNS | GC COLUMNS

11
10
4
2
3
11 12
10

1.5
1 4
9 13
29.5 30 30.5 2 3 31 5 31.5 7
31.75 32 14
6 8
Relative Abundance

1.0 15 16
17.8 17.9 18 18.1 18.2 18.3 18.4 18.5 18.6 18.7 18.8 18.9 19 19.1

App ID 25708
11
0.5 10

1.5

0 1

0.5
15 20 25 30 35 40 45 50 min

29 29.5 30 30.5 31 31.5 31.75 32

GC-MS conditions:
Column: Zebron ZB-PAH-EU Sample:
Dimensions: 30 meter x 0.25 mm x 0.20  µm 1. Benzo[c]fluorene 9. Benzo[a]pyrene
Part No.:7HG-G043-10 2. Benz[a]anthracene 10. Indeno[1,2,3-cd]pyrene
Injection:Split 5:1 @ 330 °C, 1 μL 3. Cyclopenta[c,d]pyrene 11. Dibenz[a,h]anthracene
Recommended Liner: Zebron PLUS Single Taper Z-Liner™ 4. Chrysene 12. Benzo[g,h,i]perylene
Liner Part No.:AG2-4B13-05 (for Shimadzu® 2010 GC) 5. 5-Methylchrysene 13. Dibenzo[a,l]pyrene
Carrier Gas: Helium @ 24 psi (constant pressure) 6. Benzo[b]fluoranthene 14. Dibenzo[a,e]pyrene
Oven Program: 45 °C for 0.8 min to 200 °C @ 45 °C/min to 226 °C @ 3 °C/min 7. Benzo[k]fluoranthene 15. Dibenzo[a,i]pyrene
for 0 min to 320 °C @ 10 °C/min for 20 min 8. Benzo[j]fluoranthene 16. Dibenzo[a,h]pyrene
Detector: MSD, 50-500 m/z
Transfer Line Temperature: 300 °C
Source Temperature: 300 °C

106 Phenomenex
Zebron ™
GC Columns

ZB-PAH-EU (cont’d)
Complete Resolution of EU 15+1
and EPA 610 PAHs
Zebron ZB-EU-PAH GC column demonstrates excellent resolution
and accurate quantitation of European regulated EU 15+1 and EPA
610 PAHs.

Analysis of EU 15+1 and EPA 610 PAHs

4.0

3.5
1 3
3.0
Great Separation of All Key
2.5 PAH Isomers
2
Relative Abundance

4 18, 19, 20
2.0

1.5 10, 11, 12

17
14, 15, 16
5,6
9 21
1.0 13 22 23

App ID 25713
24

GC COLUMNS | ZEBRON UNLIMITED GC COLUMNS

7 8
0.5

5 10 15 20 25 30 35 40 min

GC-MS conditions:
Column:
Zebron ZB-PAH-EU Sample:
Dimensions:
30 meter x 0.25 mm x 0.20  µm 1. Naphthalene 9. Benzo[c]fluorene 17. Benzo[a]pyrene
Part No.:
7HG-G043-10 2. Acenaphthylene 10. Benz[a]anthracene 18. Indeno[1,2,3-cd]pyrene
Injection:
Split 5:1 @ 330 °C, 1 μL 3. Acenaphthene 11. Cyclopenta[c,d]pyrene 19. Dibenz[a,h]anthracene
Recommended Liner:
Zebron PLUS Single Taper Z-Liner™ 4. Fluorene 12. Chrysene 20. Benzo[g,h,i]perylene
Liner Part No.:
AG2-4B13-05 (for Shimadzu® 2010 GC) 5. Phenanthrene 13. 5-Methylchrysene 21. Dibenzo[a,l]pyrene
Carrier Gas:
Helium @ 24 psi (constant pressure) 6. Anthracene 14. Benzo[b]fluoranthene 22. Dibenzo[a,e]pyrene
Oven Program:
45 °C for 0.8 min to 200 °C @ 45 °C/min to 226 °C @ 3 °C/min 7. Fluoranthene 15. Benzo[k]fluoranthene 23. Dibenzo[a,i]pyrene
for 0 min to 320 °C @ 10 °C/min for 20 min 8. Pyrene 16. Benzo[j]fluoranthene 24. Dibenzo[a,h]pyrene
Detector: MSD, 50-500 m/z
Transfer Line Temperature: 300 °C
Source Temperature: 300 °C

Phenomenex 107
 Zebron ™
GC Columns

ZB-PAH-EU (cont’d)
Better Combination of Resolution and Speed
Zebron outperforms popular GC columns for the separation of EU 15+1 and EPA 610 PAHs.

Zebron ZB-PAH-EU
30 meter x 0.25 mm x 0.20 µm
TIC
3.5 14 15
1 16
3
Better Separation
3.0
and 20 % Faster!
2.5

2
4 19
2.0 18 20
25.2 25.4 25.6 25.8

1.5
10 12

App ID 25714
17
15
6,5 9 14
1.0 11 21
13 16 22 23
24
7 8
0.5

5 10 15 20 25 30 35 40 45 50 min

14 15

16
Popular Brand A
30 meter x 0.25 mm x 0.25 µm
TIC 55 min Run Time
1 29.4 29.6 29.8 30
2.0 18, 19

App ID 25785
1.5 15
17
2 4 14
16 20
1.0 12
10
ZEBRON UNLIMITED GC COLUMNS | GC COLUMNS

6,5 9 11 13
0.5 21 22 23 24
7 8

5 10 15 20 25 30 35 40 45 50 min

Popular Brand B
15
14 Co-eluting Key Isomers
30 meter x 0.25 mm x 0.10 µm 16
TIC
3.5
1 3

3.0
2
4 24 24.2 24.4 24.6 24.8
2.5
19
20
2.0 18

12
1.5 6,5 10
9 15 17
14
App ID 25786

11 16
1.0 13
21
7 22
8 23 24
0.5

5 10 15 20 25 30 35 40 45 50 min

GC-MS conditions for both applications:

Column: As Indicated Sample:
Dimension: As indicated 1. Naphthalene 9. Benzo[c]fluorene 17. Benzo[a]pyrene
Injection: Split 5:1 @ 330 °C, 1 μL 2. Acenaphthylene 10. Benz[a]anthracene 18. Indeno[1,2,3-cd]pyrene
Recommended Liner: Zebron PLUS Single Taper Z-Liner™ 3. Acenaphthene 11. Cyclopenta[c,d]pyrene 19. Dibenz[a,h]anthracene
Liner Part No.: AG2-4B13-05 (for Shimadzu 2010 GC)
®
4. Fluorene 12. Chrysene 20. Benzo[g,h,i]perylene
Carrier Gas: Helium @ 24 psi (constant pressure) 5. Phenanthrene 13. 5-Methylchrysene 21. Dibenzo[a,l]pyrene
Oven Program: 45 °C for 0.8 min to 200 °C @ 45 °C/min to 226 °C @ 3 °C/min 6. Anthracene 14. Benzo[b]fluoranthene 22. Dibenzo[a,e]pyrene
for 0 min to 320 °C @ 10 °C/min for 20 min 7. Fluoranthene 15. Benzo[k]fluoranthene 23. Dibenzo[a,i]pyrene
Detector: MSD, 50-500 m/z 8. Pyrene 16. Benzo[j]fluoranthene 24. Dibenzo[a,h]pyrene
Transfer Line Temperature: 300 °C
Source Temperature: 300 °C

Comparative separations may not be representative of all applications.

108 Phenomenex
Zebron ™
GC Columns

ZB-PAH-EU (cont’d) Column Profile

GC-MS Analysis of PAHs in Rubber and Plastic

Zebron ZB-PAH-EU GC column separates the 18 PAH isomers
within 12 minutes, resolving all critical pairs while demonstrating POLARITY MS CERTIFIED

consistent column inertness.

PROPRIETARY

4
7
8 USP PHASE TEMP LIMITS ( °C )
G51 40 to 340/360

3
5
10 Analysis of 18 PAHs in just
2 12 min!
1 6
11
17

15 Phase Chemistry
9 18
14 16 • Proprietary
12
13
Recommended Applications
App ID 25732

• Analysis of 15+1 EU-regulated and EPA

regulated PAHs in food testing, rubber, plastic,
coal
• Sources include cigarette smoke, vehicle
3 4 5 6 7 8 9 10 11 12 min
exhausts, asphalt roads, coal, coal tar,
wildfires, agricultural burning, residential
The high temperature limits of the ZB-PAH-EU wood burning, municipal, industrial waste
(340/360 °C) is extremely helpful for easy bakeout of dirty
incineration

GC COLUMNS | ZEBRON UNLIMITED GC COLUMNS

matrices and allows for eluting heavy PAHs!

Column: Zebron ZB-PAH-EU

Contact Phenomenex or your local
Dimensions: 10 meter x 0.10 mm x 0.08 µm Phenomenex distributor for additional
Part No.: 7CB-G043-59 GC products and applications.
Injection: Split 5:1 @ 320 °C, 1 μL
Recommended Liner: Zebron PLUS Single Taper Z-Liner™
Liner Part No.: AG2-4B13-05 (for Shimadzu® 2010 GC System)
Carrier Gas: Helium @ 0.68 mL/min (constant flow) Learn more about: ZB-PAH-EU
Oven Program: 100 °C for 3.0 min to 200 °C @ 60 °C/min to 270 °C @ 22 °C/min to
300 °C @ 4.5 °C/min to 330 °C @ 80 °C/min for 0.5 min
Detector: MSD, 50-500 m/z
Transfer Line Temperature: 300 °C
Source Temperature: 330 °C
Sample: 1. Naphthalene 10. Chrysene
2. Acenaphthylene 11. Benzo[b]fluoranthene
3. Acenaphthene 12. Benzo[k]fluoranthene
4. Fluorene 13. Benzo[j]fluoranthene
5. Phenanthrene 14. Benzo[a]pyrene
6. Anthracene 15. Benzo[e]pyrene
7. Fluoranthene 16. Indeno[1,2,3-cd]pyrene
8. Pyrene 17. Dibenz[a,h]anthracene
9. Benz[a]anthracene 18. Benzo[g,h,i]perylene

Ordering Information
Zebron ZB-PAH-EU GC Columns
ID (mm) df (µm) Temp. Limits ºC Part No.
10-Meter
0.10 0.08 40 to 340/360 7CB-G043-59
20-Meter
0.18 0.14 40 to 340/360 7FD-G043-47
30-Meter
0.25 0.20 40 to 340/360 7HG-G043-10
60-Meter
0.25 0.20 40 to 340/360 7KG-G043-10

Phenomenex 109
 Zebron ™
GC Columns

ZB-PAH-CT Use the column finder to replace or

upgrade an existing column:
• Enhanced resolution for chrysene and triphenylene www.phenomenex.com/FindGC
(PAH Interferences)
• Increased Benzo[b,k] fluoranthene separation
• Optimal performance and resolution of regulated
PAH isomers
• Great resolution of critical isomers,
e.g. Benzo[b,j,k]fluoranthene

Priority PAH Analysis by GC

Zebron ZB-PAH-CT columns are manufactured to provide the most optimal per-
formance for EU-regulated polycyclic aromatic hydrocarbons (PAHs) and EPA
regulated PAHs.

Excellent Resolution of Chrysene and Triphenylene

We designed the Zebron ZB-PAH-CT GC column to achieve complete resolu-
tion of Chrysene from Triphenylene along with other EU 15+1 PAH compounds.
Its unique selectivity helps eliminate false positives while resolving PAH isomers,
providing easy, fast, and accurate quantification of PAHs in environmental and
food samples.

EU 15+1 PAH Analysis Using Zebron ZB-PAH-CT

ZEBRON UNLIMITED GC COLUMNS | GC COLUMNS

Complete Separation of Separation of Benzo[b,j,k]

Triphenylene and Chrysene
9
7 8 12
5
3 4
11

20 21 22 29 40 41

4 5
12
3

13
1
11
2
8 9 15 16 17
6 7 14
10
App ID 25715

15 20 25 30 35 40 45 50 min

Column: Zebron ZB-PAH-CT Sample: 1. Benzo[c]fluorene 10. Benzo[a]pyrene

Dimensions: 40 meter x 0.18 mm x 0.14 µm 2. Cyclopenta[c,d]pyrene 11. Indeno[1,2,3-c,d]pyrene
Part No.: 7PD-G044-47 3. Benz[a]anthracene 12. Dibenz[a,h]anthracene
Injection: Split 30:1 @ 320 °C, 1 μL 4. Triphenylene 13. Benzo[g,h,i]perylene
Recommended Liner: Zebron PLUS Single Taper Z-Liner™ 5. Chrysene 14. Dibenzo[a,l]pyrene
Liner Part No.: AG2-4B13-05 (for Shimadzu® 2010 GC) 6. 5-Methylchrysene 15. Dibenzo[a,e]pyrene
Carrier Gas: Helium @ 78 psi (constant pressure) 7. Benzo[b]fluoranthene 16. Dibenzo[a,i]pyrene
Oven Program: 45 °C for 0.8 min to 200 °C @ 45 °C/min to 265 °C @ 3 °C/ min 8. Benzo[j]fluoranthene 17. Dibenzo[a,h]pyrene
for 5 min to 270 °C @ 1 °C/min to 320 °C @ 10 °C/min for 15 min 9. Benzo[k]fluoranthene
Detector: MSD (Shimadzu GC-MS-QP2010 Ultra)
Mode: SIM
SIM Ions: 216, 226, 228, 242, 252, 276, 278, 302 m/z
Transfer Line Temperature: 300 °C
Source Temperature: 300 °C

110 Phenomenex
 Zebron ™
GC Columns

ZB-PAH-CT (cont’d) Column Profile

Comparison of ZB-PAH-CT vs. Popular GC PAH column

Shorter Run Time and Better Sensitivity POLARITY MS CERTIFIED

Analysis of EU 15+1 and Triphenylene PAHs PROPRIETARY

Zebron ZB-PAH-CT
USP PHASE TEMP LIMITS ( °C )
30 meter x 0.25 mm x 0.20 µm
G51 40 to 320/340
3 5
4
Great Resolution of Chrysene
2 and Triphenylene

TIC
12
2.5
Better Sensitivity for
11 13
18 19

Late Eluters
Phase Chemistry
2.0

35 • Proprietary
4 27 % Faster Analysis
1.5
10 16
9 15 Recommended Applications
2 14
1 8 17
1.0 7
6 • Analysis of 15+1 EU-regulated and EPA
App ID 25753

0.5 regulated PAHs in food testing, rubber, plastic,

coal
0 • Sources include cigarette smoke, vehicle
exhausts, asphalt roads, coal, coal tar,
10 15 20 25 30 35 40 45 50 min
wildfires, agricultural burning, residential
wood burning, municipal, industrial waste
GC-MS conditions for both applications: incineration.

GC COLUMNS | ZEBRON UNLIMITED GC COLUMNS

Column: As Indicated Sample: 1. Benzo[c]fluorene
Dimensions: As Indicated 2. Cyclopenta[c,d]pyrene
Part No.: 7HG-G044-10 (Zebron ZB-PAH-CT) 3. Benz[a]anthracene
Injection: Split 15:1 @ 320 °C, 1 μL 4. Triphenylene Contact Phenomenex or your local
Recommended Liner: Zebron PLUS Single Taper Z-Liner™ 5. Chrysene Phenomenex distributor for additional
Liner Part No.: AG2-4B13-05 6. 5-Methylchrysene GC products and applications.
Carrier Gas: Helium @ 23.7 psi (constant pressure) 7. Benzo[b]fluoranthene
Oven Program: 45 °C for 0.8 min to 200 °C @ 45 °C/min to 8. Benzo[j]fluoranthene
266 °C @ 3 °C/ min for 0 min to 320 °C @ 9. Benzo[k]fluoranthene Extend column lifetime.
10 °C/min to 320 °C for 20 min 10. Benzo[a]pyrene Add a Z-Guard™ to your next
Detector: MSD (Shimadzu® GC-MS-QP2010 Ultra) 11. Indeno[1,2,3-c,d]pyrene Zebron GC order.
Mode: SIM 12. Dibenz[a,h]anthracene
SIM Ions: 216, 226, 228, 242, 252, 276, 278, 302 m/z 13. Benzo[g,h,i]perylene
Transfer Line Temperature: 300 °C 14. Dibenzo[a,l]pyrene
Source Temperature: 300 °C 15. Dibenzo[a,e]pyrene Zebron ZB-PAH-CT Phase details
16. Dibenzo[a,i]pyrene
17. Dibenzo[a,h]pyrene
Popular Brand A
30 meter x 0.25 mm x 0.15 μm
4 5
3

TIC 2
2.5

2.0

22 23 24 25 11,12
1.5 9 10 Ordering Information
2, 4, 5 78
App ID 25717

Zebron ZB-PAH-CT GC Columns

3
1.0 13 ID (mm) df (µm) Temp. Limits ºC Part No.
1
6 20-Meter
0.5 0.18 0.14 40 to 320/340 7FD-G044-47
14 15 1617
30-Meter
0.25 0.20 40 to 320/340 7HG-G044-10
0
40-Meter
10 15 20 25 30 35 40 45 50 min 0.18 0.14 40 to 320/340 7PD-G044-47

Comparative separations may not be representative of all applications.

Phenomenex 111
 Zebron ™
GC Columns

ZB-FAME
• Reduce traditional run times up to 75 % Upgrade to Zebron from any high cyanopropyl phase:
• Improve separation of cis/trans FAME isomers Agilent® Supelco®
• Suitable with AOAC, AOCS, and IOC methods • CP-Sil 88 • SP®-2380
• HP-88 • SP-2560
• DB®-23

Baseline Separation of Common Isomers

35 35 35

C18:3 and C20:1 C22:1, C20.3, and C20:4

RS ≥ 6.3 RS ≥ 1.5
23821
4 4 4 6 6 6 121212
3 8 8 R8SC18
≥ 2.5 for troublesome
cis/trans isomers
36 36 36
37 37 37

18 16
16 16 18 18 3333343334 34
5 5 5 23 23 23
7 7 7 9 10
9 10
911
101111
17 17 17 30 30 30
3132
3132
3132
131313 15 15 15
141414 20 19
19 19 20
21 2022 21
21 22 24
22 24252426
25 26
27 26
25 28
27 28
27 28
29 29 29

555 101010 151515 202020 25min

25min
25min
35

4 6 12
36
ZEBRON UNLIMITED GC COLUMNS | GC COLUMNS

3 8
2 37
App ID 23821

1 16 18 33 34
5 23 30
7 9 10 17
11 13 3132
15
14 19 20 21 22 24 25 26 2728
29

0 5 10 15 20 25 min

Column: Zebron ZB-FAME

Dimensions:
Part No.:
30 meter x 0.25 mm x 0.20 µm
7HG-G033-10 Easy Liner Selection
Injection: Split 50:1 @ 240 °C, 1 µL
Recommended Liner: Zebron PLUS Single Taper with Wool, 4 mm ID
Liner Part No.: AG2-0A11-05 (for Agilent® systems)
Carrier Gas: Helium @ 1.2 mL/min (constant flow)
Oven Program: 100 °C for 2 min to 140 °C @ 10 °C/min
to 190 °C @ 3 °C/min to 260 °C @
30 °C/min for 2 min
Detector: FID @ 260 °C
Sample: 37 FAME standard

Our GC liner finder tool makes liner selection

a breeze. You can even search by application,
injection type, GC system, or your current
liner part number.

www.phenomenex.com/FindLiner

112 Phenomenex
Zebron ™
GC Columns

The Fast FAME GC Column Column Profile

Traditionally, cis/trans FAME separations require the use of long (100

meters or more) columns and can run up to 60 minutes, resulting in
a bottleneck to higher productivity. Zebron ZB-FAME provides tar- POLARITY MS CERTIFIED
geted selectivity that allows for reduced column length – run times
as short as 11 minutes without compromising your results!
POLARITY MS CERTIFIED

USP PHASE TEMP LIMITS ( °C )

G48 -20 to 280
37 FAMEs In A Short 11 Minute Run USP PHASE TEMP LIMITS ( °C )
G48 -20 to 280
23839

Short 20 meter column length

saves time AND money! > 75 % reduction in run
times compared to the
traditional AOAC method!

4 Phase Chemistry
6

12
CN
CN
8
3

35
CC3 3HH
6 6

5 30
2 7
16
18
33
O
O Si
Si
9 10 36 37
1 23 31 34

CC3 HH
11 13 32
28
15 29
25 2627 6
14 17 19 20 21 22 App ID 23839 3 6
24

CN
CN
High Cyanopropyl

GC COLUMNS | ZEBRON UNLIMITED GC COLUMNS

1 2 3 4 5 6 7 8 9 10 min
High Cyanopropyl
Recommended Applications

• Fatty Acid Methyl Ester (FAMEs)

Column: Zebron ZB-FAME • cis/trans FAME isomers
Dimensions: 20 meter x 0.18 mm x 0.15 μm
• Omega 3, Omega 6 FAMEs
Part No.: 7FD-G033-05
Injection: Split 100:1 @ 250 °C, 1 µL
Recommended Liner: Zebron PLUS Single Taper Z-Liner™
Liner Part No.: AG2-0A13-05 (for Agilent® systems)
Carrier Gas: Helium @ 1.0 mL/min (constant flow)
Oven Program: 80 °C for 1.5 min to 160 °C @ 40 °C/min to
185 °C @ 5 °C/min to 260 °C @ 30 °C/min
Detector: FID @ 260 °C
Sample: 37 FAME standard

Ordering Information
Zebron ZB-FAME GC Columns
ID (mm) df (µm) Temp. Limits ºC Part No.
20-Meter
0.18 0.15 -20 to 280 7FD-G033-05
30-Meter
0.25 0.20 -20 to 280 7HG-G033-10
30-Meter with 5-Meter Guardian™ Integrated Guard
0.25 0.20 -20 to 280 7HG-G033-10-GGA
60-Meter
0.25 0.20 -20 to 280 7KG-G033-10
100-Meter
0.25 0.20 -20 to 280 7MG-G033-10
Note: If you need a 5 in. cage, contact technical support at www.phenomenex.com/chat or simply reach
out to your Technical consultant. Conditions may apply. Agilent 6850, some SRI and process GC systems
use only 5 in. cages.

Phenomenex 113
 Zebron ™
GC Columns

ZB-SemiVolatiles
Maximize Inertness Upgrade to Zebron from any 5 %-phenyl or
5 % phenyl-arylene / 95 % dimethylpolysiloxane phase:
• Specifically designed to overcome obstacles for sensitive
semi-volatiles methods Agilent® Restek® Supelco®
• DB®-5ms • HP-5ms • Rxi®-5Sil MS • SLB®-5ms
• Enviro-Inert™ Technology provides a rugged 5 % phenyl- • DB-5ms Ultra Inert • HP-5ms Ultra Inert • Rxi-5ms
arylene phase – reduce activity without compromising • DB-5.625 • VP-5ms
• DB-UI 8270D • CP-5il 8 CB MS
selectivity
• Rugged QC test includes EPA 8270 tuning standard to
ensure column is ready to pass suitability requirements
2.0 2.2 2.4 2.6 min 7.1 7.3 7.5 7.7 7.9 min

• Popular for EPA Methods 525, 610, 625, 8100, and 8270D

Improved Peak Shapes

Full Separation
Separated from
Solvent

135 Compounds in Under 16 Minutes

ZB-SemiVolatiles provides improved productivity with shorter run
times for EPA 8270D, while maintaining resolution of key critical pairs.
2.0 2.2 2.4 2.6 min 7.1 7.32.2 7.52.3 7.72.4 7.9 2.5
min min 6.1
14.8 6.3
15.0 6.5
15.2 6.7 6.9 min
15.4 min

1,4-Dioxane-D8 and N-Nitrosodimethylamine and

Semivolatile Organic Compounds 1,4-Dioxane Pyridine

16 Minute Run!
For the full compound list and
ZEBRON UNLIMITED GC COLUMNS | GC COLUMNS

identifications, visit
www.phenomenex.com/SV Valley Height = 20.9 %
8270D Requires ≤ 50 % Less Tailing
BOX THAT IS 2" TALL BY 1.6" WIDE
(USED AS TEMPLATE)

2.2 2.3 2.4 2.5 min 6.1 6.3 6.5 6.7 6.9 min 12.1 12.3 12.5 12.7 12.9 min
App ID 20582

2.0 2.2 2.4 2.6 min 7.1 7.3 7.5 7.7 7.9 min 14.8 15.0 2.0
15.2 2.2
15.4 min2.4 2.6 min 7.1 7.3 7.5 7.7 7.9 min 14.8 15.0 15.2 15

Indeno[1,2,3-cd]pyrene and Pentachlorophenol

Dibenz[a,h]anthracene,
2.0 2.0
2.2 2.2
2.4 2.4
2.6 min2.6 min
both
7.1
share
7.1
7.3 7.3
7.5
mass
7.5
7.7
276
7.9 min 7.9 min
7.7 14.8 14.8
15.0 15.0
15.2 15.2 15.4 min
15.4 min

2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 min

BOX THAT IS 2" TALL BY 1.6" WIDE

(USED AS TEMPLATE)

Column: Zebron ZB-SemiVolatiles Good Peak Shape Valley Height = 9.9 %

Dimensions: 30 meter x 0.25 mm x 0.25 µm 8270D Requires ≤ 50 %
Part No.:7HG-G027-11
Injection:Split 10:1 @ 280 °C, 1 µL
Liner:AG0-8499 (Single Taper with Wool)
Septum: AG0-4697
2.2 2.3 (PhenoRed
2.4 ™
2.5 min -400) 6.1 6.3 6.5 6.7 6.9 min 12.1 12.3 12.5 2.2 12.7 2.3 12.9 min
2.4 2.5 min 6.1 6.3 6.5 6.7 6.9 min 12.1 12.3 12.5 12.7 1

Inlet Seal:AG0-8620 (Easy Seals™ Inlet Base Seal)

Carrier Gas: Helium @ 1.4 mL/min (constant flow)
Oven Program: 40 °C for 0.5 min to 260 °C @ 40 °C/min to 295 °C @
2.2 2.2
2.3 2.3
2.4 2.4
2.5 min2.5 min 6.1 6.1
6.3 6.3
6.5 6.5
6.7 6.7
6.9 min6.9 min 12.1 12.1
12.3 12.3
12.5 12.5
12.7 12.7
12.9 min12.9 min
6 °C/min to 325 °C @ 25 °C/min for 2 min
Detector: MSD @ 340 °C; 45 – 450 amu 2,4-Dinitrophenol Benzo[b]fluoranthene and
Sample: Analytes are 25 ppm in Dichloromethane Benzo[k]fluoranthene
135 compounds in EPA Method 8270D

BOX THAT IS 2" TALL BY 1.6" WIDE BOX THAT IS 2" TALL BY 1.6" WIDE
(USED AS TEMPLATE) (USED AS TEMPLATE)

BOXISTHAT
BOX THAT IS 2"
2" TALL BYTALL
1.6" BY 1.6" WIDE
WIDE
(USED
(USED AS AS TEMPLATE)
TEMPLATE)

114 Phenomenex
Zebron ™
GC Columns

ZB-SemiVolatiles Column Profile

We QC Test For the Compounds You Analyze

We take the guesswork out of meeting method requirements by POLARITY MS CERTIFIED
aggressively testing ZB-SemiVolatiles with two different test mixes.
We incorporated troublesome analytes from your samples and
8
compounds in the EPA 8270D tuning standard into our QC test, so POLARITY MS CERTIFIED

you can be sure your column is ready to meet suitability require-

USP PHASE TEMP LIMITS ( °C )
ments for the method. 8G27 -60 to 325/350

USP PHASE TEMP LIMITS ( °C )

G27 -60 to 325/350

Meet Requirements Out-of-the-Box

Test Probe Criteria EPA Requirement Our Requirement
Pyridine
Very active amine that exposes even the
smallest amount of column activity. This
Peak Response Not Specified ≥ 0.6 Engineered Self Cross-linking™ (ESC)
ensures that our Enviro-Inert™ deactivated
column performs at the highest possible
Phase Chemistry
level for difficult basic compounds.
Pentachlorophenol 5 % 5Phenyl-Arylene
% Phenyl-Arylene
Disappears and tails on active columns; it Peak Skew ≤ 2.0 ≤ 2.0
is important to measure relative response
and peak skew criteria.
Peak Response Not Specified ≥ 0.3
CHCH
3
3
CH
CH 3
3
CH3
CH3
Benzidine Si Si O Si O
Active amine that tails when column
Peak Skew ≤ 2.0 ≤ 2.0 Si Si O Si O
activity is present, complicating peak CH 3 CH3 CH 3
quantification.
DDT CH 3 CH3 CH 3
95 % Dimethylpolysiloxane
Breaks down in an active system to DDE
Recommended Applications
95 % Dimethylpolysiloxane
and DDD. With our QC test, you are as- Breakdown < 20 % < 20 %
sured that your column will meet the EPA
requirements upon installation. • Semivolatiles (SVOCs)

GC COLUMNS | ZEBRON UNLIMITED GC COLUMNS

Injection • EPA Methods (525, 610, 625, 8100, 8270D)
To ensure trace-level sensitivity, QC is per-
formed with a 20 ppm mix using a 100:1 Sensitivity
50 ng or less 0.2 ng • PAHs
on column on column
split injection – effectively 250 times less • PBDEs
than the EPA maximum allowed.

Contact Phenomenex or your local

Stands Up to Tough Samples for Increased Lifetime Phenomenex distributor for additional
GC products and applications.

“ I have found the Phenomenex ZB-

SemiVolatiles columns to be superior in
Ordering Information
Zebron ZB-SemiVolatiles GC Columns
quality and durability than any other columns ID (mm) df (µm) Temp. Limits ºC Part No.
we have previously used. 15-Meter
0.25 0.25 -60 to 325/350 7EG-G027-11
The columns not only last longer, but the 0.25 0.50 -60 to 325/350 7EG-G027-17
reproducibility of column is extraordinary. 20-Meter
0.18 0.18 -60 to 325/350 7FD-G027-08
The column holds calibrations particularly 0.18 0.36 -60 to 325/350 7FD-G027-53
well, even after multiple injections of samples 30-Meter
with far less than desirable matrices. All 0.25 0.25 -60 to 325/350 7HG-G027-11
0.25 0.50 -60 to 325/350 7HG-G027-17
of this equates to less downtime and “ 0.32 0.25 -60 to 325/350 7HM-G027-11
maintenance and more productivity for 30-Meter with 5-Meter Guardian™ Integrated Guard
TestAmerica. 0.25 0.25 -60 to 325/350 7HG-G027-11-GGA
0.25 0.50 -60 to 325/350 7HG-G027-17-GGA
30-Meter with 10-Meter Guardian Integrated Guard
Ryan McKernan, GC-MS Semi-Volatile Analyst
0.25 0.25 -60 to 325/350 7HG-G027-11-GGC
TestAmerica Laboratories, Inc. Buffalo 0.25 0.50 -60 to 325/350 7HG-G027-17-GGC
60-Meter
0.25 0.25 -60 to 325/350 7KG-G027-11
Note: If you need a 5 in. cage, contact technical support at www.phenomenex.com/chat or simply
The opinions stated herein are solely those of the speaker reach out to your Technical consultant. Conditions may apply. Agilent 6850, some SRI and process
and not necessarily those of any company or organization. GC systems use only 5 in. cages.

Phenomenex 115
 Zebron ™
GC Columns

ZB-CLPesticides-1 and -2

7 EPA Methods, One Column Set Upgrade to Zebron from these similar* phases:
• Guaranteed alternative to Restek Rtx-CLPesticides Restek®
• Rtx®-CLPesticides • Stx®-CLPesticides
• Optimized, versatile selectivity for chlorinated pesticides • Rtx-CLPesticides2 • Stx-CLPesticides2
and herbicides
*not exact equivalent, selectivity may differ
• Well-suited for dual-column configurations using GC-ECD
• Run EPA Methods 8081 and 8081 extended, 8082, 8151, 504,
505, 508, and 552 without changing columns – save time

Direct Replacement for Restek

Rtx-CLPesticides Phases
You asked for optimized performance for pesticides by GC-ECD
detectors, without time-consuming method development. We’ve
delivered a direct replacement**! ZB-CLPesticides-1 and -2 provide
guaranteed drop-in performance compared to your current Rtx-
CLPesticides column set, without the hassle.

Zebron
ZB-CLPesticides-1 ZB-CLPesticides-2
22631 22632
30 m x 0.32 mm x 0.32 µm 30 m x 0.32 mm x 0.25 µm
2 2
3 5 5
Equivalent Elution 3
10 12
9 Profiles 11 21
6 7 10 13 21 6 7 13 15 17 22
8 12 141617 89
1 11 15 1 14 16 19
19 22
ZEBRON UNLIMITED GC COLUMNS | GC COLUMNS

18 18

4 4
App ID 22631

App ID 22632
20 20

2 3 4 5 6 7 min 2 3 4 5 6 7 min

Restek®
Rtx®-CLPesticides Rtx-CLPesticides2
22634
22633
30 m x 0.32 mm x 0.32 µm 30 m x 0.32 mm x 0.25 µm
2 3 5 2

12 3
6 7 10 14 5
16 10 12
13 15 17 21
1
9
8 11 22 6 7 11 13 1516
1 89 21
19 14 17 19
18 22
18
4 4
20
App ID 22633

App ID 22634

20

2 3 4 5 6 7 min 2 3 4 5 6 7 min

Conditions for all columns:

Columns: As listed Detector: ECD @ 330 °C
Dimensions: As listed Y-Connector: AG0-4717 (Fused Quartz)
Part No.: 7HM-G028-51 (ZB-CLPesticides-1) Guard Column: 7AM-G000-00-GZ0 (5 m Z-Guard™)
7HM-G029-11 (ZB-CLPesticides-2) Liner: AG0-8499 (Single Taper with Wool at Bottom)
Injection: Splitless (hold 0.3 min) @ 250 °C, 1 µL Septum: AG0-4696 (PhenoRed™-400)
Carrier Gas: Helium @ 3.9 mL/min (constant flow) Inlet Seal: AG0-8620 (Gold-Plated Easy Seals™)
Oven Program: 120 °C to 200 ° C @ 45 °C/min to 230 °C @ Sample: Analytes are 250 ng/mL in hexane.
15 °C/min to 330 °C @ 30 °C/min for 2 min
See page 117 for compound list.
**Direct replacement: this category indicates an alternative column which will likely give a similar selectivity.
Conditions for each method were the same for all columns tested.
Comparative separations may not be representative of all applications.

116 Phenomenex
Zebron ™
GC Columns

ZB-CLPesticides-1 and -2 Column Profile

Five-Point Calibration Curve at 5‚ 15‚ 25‚ 100‚ and 250 ng/mL CLP-1

Peak ZB-CLPesticides-1 ZB-CLPesticides-2 US EPA
No. Analyte % RSD* % RSD* Specifications POLARITY*
1 2‚4‚5‚6-TCMX (Surr) 3.8 3.0 < 20
2 a-BHC 8.3 3.8 < 20
3 g-BHC 5.9 5.6 < 20
4 b-BHC 6.9 6.9 < 20
TEMP LIMITS ( °C )
5 d-BHC 4.9 5.7 < 20 40 to 320/340
6 Heptachlor 8.0 6.5 < 20
7 Aldrin 4.2 2.3 < 20
8 Heptachlor epoxide 3.8 2.3 < 20
9 trans-Chlordane 4.1 3.8 < 20
10 cis-Chlordane 4.0 3.3 < 20
11 4‚4'-DDE 4.8 2.9 < 20 *Similar polarity to ZB-35.
12 Endosulfan I 6.0 2.5 < 20
13 Dieldrin 7.7 4.9 < 20 CLP-2
14 Endrin 9.4 6.6 < 20
POLARITY**
15 4‚4’-DDD 9.2 3.6 < 20
16 Endosulfan II 6.6 4.1 < 20
17 4‚4’-DDT 11.6 6.9 < 20
18 Endrin aldehyde 8.3 7.3 < 20
19 Endosulfan sulfate 8.0 7.1 < 20 TEMP LIMITS ( °C )
20 Methoxychlor 6.7 6.1 < 20 40 to 320/340

21 Endrin ketone 6.5 7.2 < 20

22 Decachlorobiphenyl (Surr) 6.7 6.6 < 20
Average 6.6 % 4.9 % < 20
*Calculated using response factors as per EPA guidelines

**Similar polarity to ZB-MultiResidue-2

GC COLUMNS | ZEBRON UNLIMITED GC COLUMNS

Phase Chemistry
ZB-CLPesticides GC Column Kits
• Proprietary
Ordering Information
0.25 mm ID Kit
(includes 1 of each below) Part No.: KG0-9285 Recommended Applications
Description Dimension Part No.
ZB-CLPesticides-1 30 meter x 0.25 mm x 0.25 µm 7HG-G028-11
• Dual-Column Chlorinated Pesticide Methods
ZB-CLPesticides-2 30 meter x 0.25 mm x 0.20 µm 7HG-G029-10 • EPA Methods (8081 and 8081 extended,
Z-Guard™ Column 5 meter x 0.25 mm 7AG-G000-00-GZ0 8082, 8151,504, 505, 508, 552)
Y-Connector Fused Quartz AG0-4717
Polyimide Resin 0.5 mL, rated to 350 °C AG0-5722
0.32 mm ID Kit
(includes 1 of each below) Part No.: KG0-9286
Description Dimension Part No.
ZB-CLPesticides-1 30 meter x 0.32 mm x 0.32 µm 7HM-G028-51 ZB-CLPesticides GC Columns
ZB-CLPesticides-2 30 meter x 0.32 mm x 0.25 µm 7HM-G029-11 Ordering Information
Z-Guard Column 5 meter x 0.32 mm 7AM-G000-00-GZ0
ZB-CLPesticides-1 GC Columns
Y-Connector Fused Quartz AG0-4717 ID (mm) df (µm) Temp. Limits ºC Part No.
Polyimide Resin 0.5 mL, rated to 350 °C AG0-5722 30-Meter
0.53 mm ID Kit 0.25 0.25 40 to 320/340 7HG-G028-11
(includes 1 of each below) Part No.: KG0-9290
0.32 0.32 40 to 320/340 7HM-G028-51
Description Dimension Part No.
0.32 0.50 40 to 320/340 7HM-G028-17
ZB-CLPesticides-1 30 meter x 0.53 mm x 0.50 µm 7HK-G028-17
0.53 0.50 40 to 320/340 7HK-G028-17
ZB-CLPesticides-2 30 meter x 0.53 mm x 0.42 µm 7HK-G029-16
Z-Guard Column 5 meter x 0.53 mm 7AK-G000-00-GZ0 ZB-CLPesticides-2 GC Columns
Y-Connector Fused Quartz AG0-4717 ID (mm) df (µm) Temp. Limits ºC Part No.
Polyimide Resin 0.5 mL, rated to 350 °C AG0-5722 30-Meter
0.25 0.20 40 to 320/340 7HG-G029-10
0.32 0.25 40 to 320/340 7HM-G029-11
0.32 0.50 40 to 320/340 7HM-G029-17
Contact Phenomenex or your local Phenomenex 0.53 0.42 40 to 320/340 7HK-G029-16
distributor for additional GC products and applications.

Phenomenex 117
 Zebron ™
GC Columns

ZB-MultiResidue™-1 and -2

Optimized Selectivity for Pesticides

• Specially designed for the separation of all types of
pesticides, herbicides, and insecticides
• Baseline resolution and confirmation of all 20 chlorinated Upgrade to Zebron from these similar* phases:
pesticides regulated under EPA Method 8081 in ≤ 10 min Agilent® Restek®
• DB®-CLP1 • Rtx®-CLPesticides
• Decreased breakdown of sensitive pesticides such as DDT • DB-CLP2 • Rtx-CLPesticides2
• Stx®-CLPesticides
• Robust performance for high temperature bakeouts • Stx-CLPesticides2
• Low bleed performance for pesticide confirmation by MS *not exact equivalent, selectivity may differ

Improved Multi-Residue Pesticide

Screening by GC-MS
21 24 25 36,37 41-43 61 64-66 80-81 84
39 44-45
71-74

63
69 79
33 75
85
20 23 46-47
38
35 50
26
77,78
52 82
40 76
22 60
27 49
31 62 67 86 87
59
ZEBRON UNLIMITED GC COLUMNS | GC COLUMNS

19
29 30 32
48
58 68 70
34 51
28
18
83

14 16 18 min 22 24 min

17
13-15
4 For a complete list of all 112 compounds,
57
visit
www.phenomenex.com/MR
94,95

12
96

105
89 108
98 104
6,7
8
55 107
90 97 99 103 110,111
56
3 5 100 102
App ID 16184

112
10 54 91 92, 109
1
53 88 93 101 106
2 9 11 16

8 10 12 14 16 18 20 22 24 26 28 30 32 34 min

Column: Zebron MultiResidue™-1

Dimensions: 30 meter x 0.25 mm x 0.25 µm
Part No.: 7HG-G016-11
Injection: Splitless @ 260 °C, 1 µL
Carrier Gas: Helium @ 0.9 mL/min (constant flow)
Oven Program: 80 °C for 0.5 min to 150 °C @ 10 °C/min to 240 °C @
4 °C/min to 320 °C @ 15 °C/min for 3 min
Detector: MSD @ 320 °C; 45-400 amu
Sample: Analytes were 1 ppm in Dichloromethane

118 Phenomenex
Zebron ™
GC Columns

ZB-MultiResidue™-1 and -2 (cont’d) Column Profile

Resolve Common Pesticide Isomers MR-1

POLARITY MS CERTIFIED
ZB-MultiResidue optimized selectivities improve resolution of complex pesticide, herbicide,
and insecticide isomers. Our extremely stable siloxane-based polymer contains absolutely 11
no nitrogen or halogenated functionality, which can be unfriendly to NPD and ECD de-
tectors. Engineered Self-Crosslinking™ (ESC) bonding incorporates ladders into the phase TEMP LIMITS ( °C )
-60 to 320/340
backbone for low bleed and unmatched spectral integrity – even for trace-level samples.

2
3
1
4 5

MR-2
POLARITY MS CERTIFIED
App ID 16161

15
10 10.5 11 11.5 12 12.5 min TEMP LIMITS ( °C )
-60 to 320/340

2400
Acquisition Spectra 165 235
2400 165 235
2200
2200
2000
2000
m/z

1800
Low Bleed
Abundanciam/z

1800
1600
1600
No 207 bleed ions at 320 °C
Abundancia

1400

GC COLUMNS | ZEBRON UNLIMITED GC COLUMNS

1400
1200 199
1200 199
1000
1000
800
800
600
600 75
88
400
400
200
50
50
75
88 105
105 113
251
Engineered Self Cross-linking™ (ESC)
113 282
200 251 282
0
0 20
20
40
40
60
60
80
80
100
100
120
120
140
140
160
160
180
180
200
200
220
220
240
240
260
260
280
280
300
300
320
320
340
340
360
360
380
380
Phase Chemistry

2400
2400 NIST Reference Spectra 165
165
235
235 • Proprietary
2200
2200
2000
2000
m/z

1800
Abundanciam/z

1800
1600
1600
Recommended Applications
Abundancia

1400
1400
1200
1200
1000
1000
800
199
199 • Haloacetic Acids (HAAs)
800 88
600 88
600
400
400 50
105
105
123
123 • Herbicides / Insecticides
200 28 50 69 281 319 354
149
200
0
0 20
28
40 60
69
80 100 120
149
140 160 180 200 220 240 260
281

280 300
319
320 340
354
360 380 400
• Multi-Pesticide Screening
20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400
• Nitrogen Containing Pesticides
Cl Cl
Cl
• Organochlorine Pesticides
Column: Zebron ZB-MultiResidue-1 Cl • Organophosphorous Pesticides
Dimensions: 30 meter x 0.25 mm x 0.25 µm Cl Cl
• PCBs / Aroclors
Part No.: 7HG-G016-11
Injection: Splitless (hold 0.5 min) @ 260 °C, 1 µL
Cl Cl
Carrier Gas: Helium @ 0.8 mL/min (constant flow)
Oven Program: 100 °C for 0.5 min to 200 °C @ 25 °C/min
Cl
to 320 °C @ 15 °C/min for 2 min Cl
Detector: MSD @ 320 °C, 45-400 amu Cl
Sample: 1. o,p-DDE Cl
Cl Cl
4. o,p-DDT
Cl
Cl
Cl Cl Cl

Cl
Cl
Cl Cl
2. p,p-DDE 5. p,p-DDD Cl Cl

Cl Cl
Cl Cl
Cl Cl
3. o,p-DDD Cl
6. p,p-DDT
Cl
Cl
Cl Cl
Cl Cl
Cl
Cl Cl Cl

Cl

Cl Cl

Phenomenex 119
Cl Cl
Cl Cl
 Zebron ™
GC Columns

ZB-MultiResidue™-1 and -2 (cont’d)

Great
16181 Results for Organophosphate Pesticides
45
37
44
MR-1
14,15 41
42 43
47
17
7 40
33
38 46
6 20 30 39
48
2 11 36
1 19 24 29
8 27 31 34
25 35
21 23
4 13
28

App ID 16181
22
16 26
10 12

5 18
9 32

4 6 8 10 12 14 16 18 20 min

Zebron
MR-1
16182 Injector
MR-2 ECD Detector
16,17

Y-Splitter
ZEBRON UNLIMITED GC COLUMNS | GC COLUMNS

45
14 37 47
44 5m ECD Detector
Z-Guard
7 43
41 42 Zebron
6 15 27,28
33 48 MR-2
20 31,32
2 40 46
11
19 29
8 12 24
34
1
2123
25 30
36 38 39
4
13 35
22
App ID 16182

10 26

5
9 18
3

4 6 8 10 12 14 16 18 20 min

Conditions for both column

Columns: Zebron MultiResidue-1 Sample: Analytes are 2 ppm in Dichloromethane.
Zebron MultiResidue-2 1. Dichlorvos 17. Fonofos 33. Chlorfenvinphos
Dimensions: 30 meter x 0.32 mm x 0.50 µm 2. Mevinphos 18. Phosphamidon Isomer 34. Crotoxyphos
30 meter x 0.32 mm x 0.25 µm 3. Trichlorfon 19. Disulfoton 35. Stirofos
Part No.: 7HM-G016-17 4. TEPP (Tetraethyl 20. Dichlofenthion 36. Tokuthion
7HM-G017-11 Pyrophosphate) 21. Phosphamidon 37. Merphos Oxide (Tribufos)
Injection: On-Column @ 103 °C, 1 µL 5. Demeton Isomer 22. Chlorpyrifos Methyl 38. Ethion
Carrier Gas: Helium @ 2.8 mL/min (constant flow) 6. Thionazin 23. Ronnel 39. Fensulfothion
Oven Program: 100 °C for 0.5 min to 180 °C @ 20 °C/min to 240 °C @ 6 °C/min to 7. Ethoprop 24. Aspon 40. Contaminant
320 °C @ 15 °C/min for 2 min 8. Sulfotep 25. Methyl Parathion 41. Carbophenothion
Detector: FID @ 340 °C 9. Naled 26. Malathion 42. Famfur
Note: Columns connected using a 5 m Z-Guard™ Column and a ‘Y’ splitter. 10. Dicrotophos 27. Fenitrothion 43. EPN
11. Phorate 28. Chlorpyrifos 44. Phosmet
12. Monocrotophos 29. Fenthion 45. Leptophos
13. Demeton 30. Trichloronate 46. Azinphos Methyl
14. Terbufos 31. Parathion 47. Azinphos Ethyl
15. Diazinon 32. Merphos 48. Coumaphos
16. Dimethoate

120 Phenomenex
Zebron ™
GC Columns

ZB-MultiResidue™-1 and -2 (cont’d)

Ordering Information
Zebron ZB-MultiResidue -1 GC Columns
ID(mm) df(µm) Temp. Limits °C Part No.
20-Meter
0.18 0.18 -60 to 320/340 7FD-G016-08
30-Meter
0.25 0.25 -60 to 320/340 7HG-G016-11
0.32 0.25 -60 to 320/340 7HM-G016-11
0.32 0.50 -60 to 320/340 7HM-G016-17
0.53 0.50 -60 to 320/340 7HK-G016-17

Ordering Information
Zebron ZB-MultiResidue -2 GC Columns
ID(mm) df(µm) Temp. Limits °C Part No.
30-Meter
0.25 0.20 -60 to 320/340 7HG-G017-10
0.32 0.25 -60 to 320/340 7HM-G017-11
0.53 0.50 -60 to 320/340 7HK-G017-17
Note: If you need a 5 in. cage, contact technical support at www.phenomenex.com/chat or simply reach
out to your Technical consultant. Conditions may apply. Agilent 6850, some SRI and process GC systems
use only 5 in. cages.

ZB-MultiResidue Column Kits

Ordering Information

GC COLUMNS | ZEBRON UNLIMITED GC COLUMNS

0.25 mm ID
(kit consists of products below) Part No.: KG0-8237
Description Dimension Part No.
ZB-MultiResidue-1 Column 30 meter x 0.25 mm x 0.25 µm df 7HG-G016-11
ZB-MultiResidue-2 Column 30 meter x 0.25 mm x 0.20 µm df 7HG-G017-10
Z-Guard™ 5 meter x 0.25 mm 7AG-G000-00-GZ0
Universal Capillary Column Y-connector, — AG0-4717
Fused Quartz
Polyimide Resin 0.5 mL, rated to 350 °C AG0-5722
0.32 mm ID
(kit consists of products below) Part No.: KG0-8238
Description Dimension Part No.
ZB-MultiResidue-1 Column 30 meter x 0.32 mm x 0.50 µm df 7HM-G016-17
ZB-MultiResidue-2 Column 30 meter x 0.32 mm x 0.25 µm df 7HM-G017-11
Z-Guard 5 meter x 0.32 mm 7AM-G000-00-GZ0
Universal Capillary Column Y-connector, — AG0-4717
Fused Quartz
Polyimide Resin 0.5 mL, rated to 350 °C AG0-5722
0.53 mm ID
(kit consists of products below) Part No.: KG0-8239
Description Dimension Part No.
ZB-MultiResidue-1 Column 30 meter x 0.53 mm x 0.50 µm df 7HK-G016-17
ZB-MultiResidue-2 Column 30 meter x 0.53 mm x 0.50 µm df 7HK-G017-17
Z-Guard 5 meter x 0.53 mm 7AK-G000-00-GZ0
Universal Capillary Column Y-connector,
— AG0-4717
Fused Quartz
Polyimide Resin 0.5 mL, rated to 350 °C AG0-5722

Contact Phenomenex or your local Phenomenex distributor

for additional GC products and applications.

Extend column lifetime. Add a Z-Guard™

to your next Zebron GC order.

Phenomenex 121
 Zebron ™
GC Columns

ZB-Bioethanol

Quicker Bioethanol Testing

• Specially designed for fast and accurate bioethanol testing

• Provides accurate and reproducible results for Certificate of
Analysis (COA)
• Resolve methanol and ethanol from all other denaturant
peaks
• Great resolution of fusel alcohols
• Allows for quick bake out in between runs to eliminate
contaminates

Upgrade to Zebron from traditional phases used for bioethanol:

Agilent® Restek® SGE® Supelco®
• DB®-1 • Rtx®-1 • BP1 • SPB®-1
• HP-1 • Rxi®-1ms • SE-30
• CP-Sil 5 CB

Fast, Accurate Analysis

Determination of Denatured Bioethanol: ASTM Method D5501

3
ZEBRON UNLIMITED GC COLUMNS | GC COLUMNS

16668

2 3
2

5 min Analysis!
Speed It Up!
App ID 16663

App ID 16668
1
1

5 10 15 min 0 202 min 4 6 8 min

Column: Zebron ZB-1 Column: Zebron ZB-Bioethanol

Dimensions: 100 meter x 0.25 mm x 0.50 µm Dimensions: 15 meter x 0.25 mm x 1.00 µm
Part No.: 7MG-G001-17 Part No.: 7EG-G020-22
Injection: Split 50:1 @ 300 °C, 1 µL Injection: Split 50:1 @ 300 °C, 1 µL
Carrier Gas: Helium @ 35 cm/sec (constant flow) Carrier Gas: Hydrogen @ 25 cm/sec (constant flow)
Oven Program: 45 °C for 7 min to 255 °C @ 30 °C/min for 6 min Oven Program: 55 °C for 1.7 min to 260 °C @ 40 °C/min (hold 2.67 min)
Detector: FID @ 300 °C Detector: FID @ 300 °C
Instrument: Shimadzu® GC-2010 with Flame Ionization Instrument: Shimadzu® GC-2010 with Flame Ionization Detection and
Sample: 1. Methanol AOC-20i Automatic Liquid
2. Ethanol Sample: 1. Methanol
3. Denaturant 2. Ethanol
3. Denaturant

122 Phenomenex
Zebron ™
GC Columns
2009 R&D 100
Award Recipient
ZB-Bioethanol Column Profile

Resolve Fusel Alcohols

16674
POLARITY* MS CERTIFIED

31
16654 600,000
Ethanol Purity 550,000
Confirmed by GC-MS TEMP LIMITS ( °C )
500,000
-60 to 340/360
450,000
400,000 45

Abundance
350,000
300,000
1 2 6 26 250,000
200,000
8
150,000 Engineered Self Cross-linking™ (ESC)
100,000
Phase Chemistry
27 50,000
App ID 16674

14 0
40 80 • Proprietary
4 10 m/z
28
9 15,16 Recommended Applications
24
29 • Alcohols
5 • Ethanol Testing
22
21 • Fusel Alcohols
App ID 16654

11
3 12 23
7 13 17 18 20 25
19

2 4 6 8 10 12 min Contact Phenomenex or your local

Phenomenex distributor for additional

GC COLUMNS | ZEBRON UNLIMITED GC COLUMNS

GC products and applications.
Column: Zebron ZB-Bioethanol
Dimensions: 30 meter x 0.25 mm x 1.00 μm
Part No.: 7HG-G020-22 For Bioethanol fermentation
Injection: Split 100:1 @ 240 °C, 0.1 μL monitoring, use
Carrier Gas: Helium @ 1.2 mL/min (constant flow) Rezex-ROA HPLC columns, see p. 325
Oven Program: 40 °C for 5 min to 300 °C @ 25 °C/min
Detector: MSD @ 230 °C; 30-450 amu
Sample: 1. Methanol 17. Methylcyclopentane
2. Ethanol 18. 2,4-Dimethylpentane
3. Acrolein 19. Benzene
4. Acetone 20. Cyclohexane
5. 2-Methylbutane 21. 2-Methylhexane
6. Isopropyl alcohol 22. 2,3-Dimethylpentane
7. Pentane 23. 3-Methylhexane
8. t-Butanol 24. 2,2,4-Trimethylpentane
9. Allyl alcohol 25. Heptane
10. n-Propanol 26. Acetal
11. 2,3-Dimethylbutane 27. Toluene
12. 2-Methylpentane 28. Xylene
13. 3-Methylpentane 29. Trimethylbenzene
14. 2-Butanol
15. Ethyl acetate
16. Hexane

Ordering Information
Zebron ZB-Bioethanol GC Columns
ID(mm) df(µm) Temp. Limits °C Part No.
15-Meter
0.25 1.00 -60 to 340/360 7EG-G020-22
30-Meter
0.25 1.00 -60 to 340/360 7HG-G020-22
Note: If you need a 5 in. cage, contact technical support at www.phenomenex.com/chat or simply reach out to your
Technical consultant. Conditions may apply. Agilent 6850, some SRI and process GC systems use only 5 in. cages.

Phenomenex 123
 Zebron ™
GC Columns

ZB-1XT SimDist

High Efficiency Metal Column Performance

• Glass Infusion™ technology for higher efficiency and Upgrade to Zebron from any 100 % dimethylpolysiloxane phase:
greater column-to-column reproducibility
Agilent® Restek® SGE®
• Individual QC testing for every column • DB®-1 • Rtx®-1 • BP1
• DB-HT SimDis • Rxi®-1HT • BPX1-SimD
• Up to 70 % higher efficiency than other columns • DB-PS1 • MXT®-1HT SimDist
• DB-PS2887
• Increased accuracy for high temperature • CP-SimDist
simulated distillation • CP-SimDist UltiMetal

Glass Infusion Technology for Improved Performance

ZEBRON UNLIMITED GC COLUMNS | GC COLUMNS

Multi-layer Glass Infusion

ensures a thick uniform surface that is consistent
throughout the length of the column.

Due to the Glass Infusion, we achieve complete surface

coverage with our advanced high temperature polymer.

Rugged Metal Tubing

allows you to run methods up to 450 °C without
becoming brittle or breaking.

124 Phenomenex
Zebron ™
GC Columns

ZB-1XT SimDist Column Profile

Improve
19264
Results for Simulated Distillation
POLARITY MS CERTIFIED

POLARITY MS CERTIFIED
Hydrocarbons C7–C100+: ASTM Method D7169
5
5
USP PHASE TEMP LIMITS ( °C )
C7

USP
G1, G2, PHASE
G9, G38 -60 toLIMITS
TEMP 450* ( °C )
G1, G2, G9, G38 -60 to 450*
High Efficiency!
C8

Resolution: 4.81
C9

C44
C50
C52

*Thicker
*Thickerfilm
film(2.65
(2.65 µm) is rated
µm) is ratedtoto400
400°C.
°C.

Engineered Self Cross-linking™ (ESC)

Phase Chemistry

CH
CH
C60

3
3

Si O
C70

App ID 19264

Si O
C80

C100

CH 3
C90

3
CH
100 % Dimethylpolysiloxane
0 10 20 30 40 min
100 % Dimethylpolysiloxane
Recommended Applications

Column: Zebron ZB-1XT SimDist • ASTM Methods (D2887, D3710, D6352, D7169)

GC COLUMNS | ZEBRON UNLIMITED GC COLUMNS

Dimensions: 5 meter x 0.53 mm x 0.15 µm
Part No.: 7AK-G026-05 • Crude Oil
Injection: On-Column @ 33 °C, 1 µL • Gasoline Fractions
Carrier Gas: Helium @ 7 mL/min (constant flow)
Oven Program: 30 °C to 450 °C @ 10 °C/min for 10 min • Petroleum Distillates
Detector: FID @ 450 °C
Sample: C7 to C44 hydrocarbons and POLYWAX® 655 in CS2 • Petroleum Fractions
Note: Chromatogram is baseline subtracted.
• Simulated Distillation
• Vacuum Distillates

ZB-1XT SimDist Test Mix

Part No.: AG0-8645
Ordering Information
Zebron ZB-1XT SimDist GC Columns
ID(mm) df(µm) Temp. Limits °C Part No. Contact Phenomenex or your local
5-Meter Phenomenex distributor for additional
GC products and applications.
0.53 0.09 -60 to 450 7AK-G026-55
0.53 0.15 -60 to 450 7AK-G026-05
0.53 0.88 -60 to 450 7AK-G046-49 Guard Column Connections
5-Meter with 2-Meter Guardian Integrated Guard
™
SilTite™ Mini-Unions for
0.53 0.09 -60 to 450 7AK-G026-55-GGT 0.8 mm ID columns (P/N: AG0-8825)
and
0.53 0.15 -60 to 450 7AK-G026-05-GGT
Replacement Ferrules (P/N: AG0-8824)
10-Meter
0.53 0.15 -60 to 450 7CK-G026-05
0.53 0.88 -60 to 450 7CK-G026-49
0.53 2.65 -60 to 400 7CK-G026-35
10-Meter with 5-Meter Guardian Integrated Guard
0.53 2.65 -60 to 450 7CK-G026-35-GGA
15-Meter
0.53 0.25 -60 to 450 7EK-G026-11
Note: If you need a 5 in. cage, contact technical support at www.phenomenex.com/chat or simply reach out to your
Technical consultant. Conditions may apply. Agilent 6850, some SRI and process GC systems use only 5 in. cages.

Phenomenex 125
 Zebron ™
GC Columns

ZB-DHA-PONA

• Excellent peak shape for polar and nonpolar compounds Upgrade to Zebron from any 100 % dimethylpolysiloxane phase:
• Temperature stability and flexibility
Agilent® Restek® Supelco®
• Highly efficient dimension and consistent film thickness • HP-PONA • Rtx®-DHA • Petrocol®-DH
delivers excellent separation of paraffins, iso-paraffins, • DB®-PETRO
• CP-Sil PONA CB
olefins, naphthenes, aromatics and polar compounds
• Extensive ESC™ provides intact stationary phase and MS
certified low bleed
• Well-suited for true boiling point separation
• Excellent resolving power for critical pairs in complex
petrochemical samples

The Choice
for PIONA, DHA and PONA
Zebron ZB-DHA-PONA is the choice for the analysis of Detailed
Hydrocarbon Analysis (DHA) within the fuel industry. The Engi-
neered Self Cross-linking™(ESC) stationary phase provides low
bleed and exceptional column life for separation of DHA critical
pairs with symmetric peaks. In addition, Zebron ZB-DHA-PONA GC
columns provide excellent response and peak symmetry for polar
oxygenates.
The Zebron ZB-DHA-PONA GC column provides excellent separa-
tion of DHA critical pairs with symmetric peaks suitable for ASTM
ZEBRON UNLIMITED GC COLUMNS | GC COLUMNS

Method D6730, see table below.

Easy ZB-DHA-PONA Selection for Your ASTM Method

Recommended Recommended
Method Description Column Dimensions Part Number
ZB-DHA-PONA 50 m x 0.20 mm x 0.5 µm 7JE-G042-17
Standard Test Method for Detailed Analysis of Petroleum
ASTM D5134
Naphtha’s through n-Nonane by Capillary Gas Chromatography ZB-DHA-PONA 100 m x 0.25 mm x 0.5 µm 7MG-G042-17
ZB-DHA-PONA 50 m x 0.20 mm x 0.5 µm 7JE-G042-17
Standard Test Method for Analysis of Methyl Tert-Butyl Ether
ASTM D5441 ZB-DHA-PONA 100 m x 0.25 mm x 0.5 µm 7MG-G042-17
(MTBE) by GC
ZB-DHA-PONA 150 m x 0.25 mm x 1 µm 7QG-G042-22
Standard Test Method for Determination of Ethanol and ZB-DHA-PONA 100 m x 0.25 mm x 0.5 µm 7MG-G042-17
ASTM D5501 Methanol Content in Fuels Containing Greater than 20 % Ethanol
by Gas Chromatography ZB-DHA-PONA 150 m x 0.25 mm x 1 µm 7QG-G042-22

Standard Test Method for Determination of Individual

ASTM D6729 Components in Spark Ignition Engine Fuels by 100 Meter ZB-DHA-PONA 100 m x 0.25 mm x 0.5 µm 7MG-G042-17
Capillary High Resolution Gas Chromatography
Standard Test Method for Determination of Individual ZB-DHA-PONA 50 m x 0.20 mm x 0.5 µm 7JE-G042-17
Components in Spark Ignition Engine Fuels by 100– ZB-DHA-PONA 100 m x 0.25 mm x 0.5 µm 7MG-G042-17
ASTM D6730
Meter Capillary (with Pre-column) High-Resolution Gas ZB-DHA-PONA 150 m x 0.25 mm x 1 µm 7QG-G042-22
Chromatography ZB-DHA-PONA-TUNE 5 m x 0.25 mm x 1 µm 7AG-G042-22
Standard Test Method for Determination of Individual
ASTM D6733 Components in Spark Ignition Engine Fuels by 50-Meter ZB-DHA-PONA 50 m x 0.20 mm x 0.5 µm 7JE-G042-17
Capillary High Resolution Gas Chromatography

126 Phenomenex
Zebron ™
GC Columns

ZB-DHA-PONA Column Profile

Analysis of ASTM D6730 Components by GC-FID on ZB-DHA-PONA & ZB-DHA-PONA-TUNE GC Column

pA POLARITY MS CERTIFIED
9
POLARITY MS CERTIFIED
350 1
5
300 5
250 5 USP PHASE TEMP LIMITS ( °C )
USP PHASE TEMP LIMITS ( °C )
G1, G2, G9, G38 -60 to 360/370
200 G1, G2, G9, G38 -60 to 360/370
3 16
2
150 14

100 6
12
8 11

App ID 25558
25 26 27 28
50
4
7 15 18 22 23 24 29
10 13 17 19 20 21
0 *Thicker
*Thicker films(≥(≥1.0
films 1.0µm)
µm) are
are rated
ratedtoto340/360
340/360°C.°C.

10 20 30 40 50 60 70 min Engineered Self Cross-linking™ (ESC)

Phase Chemistry
pA
pA 9
30
CH3
CH3
28
350 1 26 18
300 24 20
250 5 22
19 25 Si O
Si O
200
23 20 22 23 24 26
150 14 18 21 27 28 29
100
50 4
6 7 8 11
10
12
13 15
16
CH 3
0 40 45 50 55 60 65 70 min
CH
100 % Dimethylpolysiloxane
3
4 6 8 10 12 14 16 18 20 min
100 % Applications
Recommended Dimethylpolysiloxane

Column 1 (Tuning): Zebron ZB-DHA-PONA-TUNE Recommended Column Union: AG0-4716

Phase: 5 % Phenyl 95 % Injection: Split 150:1 @ 200 °C, 0.2 μL • DHA

GC COLUMNS | ZEBRON UNLIMITED GC COLUMNS

Dimethylpolysiloxane Recommended Liner: Zebron PLUS Straight Z-Liner™ • PONA
Dimensions: 5 meter x 0.25 mm x 1.00 μm Part No.: AG2-0A03-05
Part No.: 7AG-G042-22 Carrier Gas: Hydrogen @ 2 mL/min (constant • PIONA
Column 2: Zebron ZB-DHA-PONA flow)
Phase: 100 % Dimethylpolysiloxane Oven Program: 30 °C for 8.5 min, to 48 °C @ • PIANO
Dimensions: 100 meter x 0.25 mm x 0.50 μm 22 °C/min for 27 min, to 141 °C • ASTM D5134, D5441, D5501, D6729, D6730
Part No.: 7MG-G042-17 @ 3 °C/min for 1 min, to 275 °C
@ 1 °C/min for 2 min
and D6733
Detection: Flame Ionization (FID) @ 275 °C

Sample: 1. Ethanol 16. C8 (n-octane)

2. C5 (n-pentane) 17. Ethylbenzene
3. 2-Methylbutane 18. 2,3-Dimethylheptane Zebron GC Columns MS Certification,
4. Tert-Butanol 19. p-Xylene see p. 437
5. 2,3-Dimethylbutane 20. C9 (n-nonane)
6. Methyl tert-butyl ether (MTBE) 21. 5-Methylnonane
7. C6 (n-hexane) 22. 1-Methyl-2-ethylbenzene
8. 1-Methylcyclopentene 23. C10 (n-decane) Contact Phenomenex or your local
9. Benzene 24. C11 (undecane) Phenomenex distributor for additional
10. Cyclohexane 25. 1,2,3,5-Tetramethylbenzene GC products and applications.
11. 3-Ethylpentane 26. Naphthalene
12. trans-1,2-Dimethylcyclopentane 27. C12 (dodecane)
13. C7 (n-heptane) 28. 1-Methylnaphthalene Extend column lifetime.
14. 2,3,3-Trimethylpentane 29. C13 (Tridecane) Add a Z-Guard™ to your next
15. Toluene Zebron GC order.

Ordering Information
Zebron ZB-DHA-PONA GC Columns
ID(mm) df(µm) Temp. Limits °C Part No.
5-Meter
0.25 1.00 -60 to 340/360 7AG-G042-22
50-Meter
0.20 0.50 -60 to 360/370 7GE-G042-17
100-Meter
0.25 0.50 -60 to 360/370 7MG-G042-17
150-Meter
0.25 1.00 -60 to 340/360 7QG-G042-22
Note: If you need a 5 in. cage, contact technical support at www.phenomenex.com/chat or simply reach out to your
Technical consultant. Conditions may apply. Agilent 6850, some SRI and process GC systems use only 5 in. cages.

Phenomenex 127
 Zebron ™
GC Columns

ZB-Drug-1

Faster Drugs of Abuse Testing Upgrade to Zebron from traditional phases used for drugs of abuse:
• Optimized phase for the separation of drugs of abuse Agilent® Restek® Supelco®
• DB®-1ms • Rxi®-1ms • SPB®-1
• Provides fast analysis with great peak shape
• DB-5ms • Rtx®-5
• Improves resolution of target analytes from matrix • DB-35 • Rtx-5ms
• Rtx-35ms
interferences
• Specially deactivated to improve quantitation for drug
compounds

Optimized Selectivity for Multiple Drug Classes

16733
Traditional 5 % Phenyl Phase Zebron ZB-Drug-1
17806
3,4

Unique Selectivity Sample: Analytes @ 10 ppm BSTFA/Pyridine

For Drugs 1. Codeine-d6
3,4
2. Codeine
1,2
3. Morphine-d3
2
4. Morphine

1
App ID 16733

App ID 17806
ZEBRON UNLIMITED GC COLUMNS | GC COLUMNS

4.7 4.8 4.9 5 5.1 5.2 5.3 5.4 5.5 5.6 5.7 5.8 5.9 6 min 4.0 4.1 4.2 4.3 4.4 4.5 4.6 4.7 4.8 min

Column: As listed Column: As listed

Dimensions: 10 meter x 0.18 mm x 0.18 µm Dimensions: 10 meter x 0.18 mm x 0.18 µm
Injection: Split 10:1 @ 240 °C, 1 µL Part No.: 7CD-G023-08
Carrier Gas: Helium @ 1.2 mL/min (constant flow) Injection: Split 10:1 @ 280 °C, 1 µL
Oven Program: 140 °C to 320 °C @ 20 °C for 1 min Carrier Gas: Helium @ 55 cm/sec (constant flow)
Detector: MSD @ 230 °C Oven Program: 180 °C to 340 °C @ 20 °C/min
Detector: MSD @ 230 °C

Common Drug Screen by GC-MS

18175

1 2 17

22,23

41
15,16
42
Sample: Analytes are 25 ppm in Methanol
14 18,19 40
30 33 39 1. Acetophenone 15. Meprobamate 29. Trimipramine
38 2. Nicotine 16. Diphenhydramine 30. Chlorcyclizine
App ID 18175

29 35
4
12
26,27 43 3. Benzocaine 17. Lidocaine 31. Cocaine
9 13 20 34 36
3
28 4. Ibuprofen 18. Hexobarbital 32. Desipramine
8
6 7 10 24
5 5. Allobarbital 19. Doxylamine 33. Codeine
21 31 37
11 25 32 6. Aprobarbital 20. Glutethimide 34. Morphine
7. Butalbital 21. Caffeine 35. Diazepam
0 8. Amobarbital 22. Chlorpheniramine 36. Hydrocodone
4 6 8 10 12 14 16 18 20 22 min
9. Phenacetin 23. Methapyrilene 37. 6-MAM
10. Pentobarbital 24. Phenobarbital 38. Oxymorphone
Column: Zebron ZB-Drug-1 11. Acetaminophen 25. Procaine 39. Heroin
Dimensions: 10 meter x 0.18 mm x 0.18 μm 12. Benzphetamine 26. Methadone 40. Fentanyl
Part No.: 7CD-G023-08 13. Secobarbital 27. Brompheniramine 41. Ibogaine
Injection: Split 10:1 @ 260 °C,1 μL 14. Phencyclidine 28. Propoxyphene 42. Triazolam
Carrier Gas: Helium @ 1 mL/min (constant flow) 43. LSD
Oven Program: 50 ºC to 150 ºC @ 15 ºC/min to 240 ºC @ 7 ºC/min to 320 ºC @ 25 ºC/min
for 2 min
Detector: MSD @ 320 °C; 45-450 amu

128 Phenomenex
Zebron ™
GC Columns
2009 R&D 100
Award Recipient
ZB-Drug-1 Column Profile

Faster Run Times and Improved Resolution POLARITY* MS CERTIFIED

Zebron ZB-Drug-1
17808
Improved
Resolution
6,7 TEMP LIMITS ( °C )
Fast Run Time
40 to 320/340

2,3

11,12

4,5 9
*Similar polarity to ZB-MultiResidue™-2.

1 Engineered Self Cross-linking™ (ESC)

App ID 17808
8 Phase Chemistry
10

• Proprietary
2.6 2.8 3 3.2 3.4 3.6 3.8 4 4.2 4.4 4.6 4.8 5 5.2 5.4 min

Recommended Applications
Column: Zebron ZB-Drug-1
Dimensions: 10 meter x 0.18 mm x 0.18 µm
Part No.:7CD-G023-08 • Drug Screening
Injection:Split 10:1 @ 280 °C, 1 µL
Carrier Gas: Helium @ 0.7 mL/min (constant flow) • 6-MAM
Oven Program: 200 °C to 210 °C @ 20 °C/min at 320 °C @ • Amphetamines
30 °C/min for 1 min
Detector: MSD @ 320 °C • Barbiturates
• Benzodiazepines

GC COLUMNS | ZEBRON UNLIMITED GC COLUMNS

Traditional Mid-Polar Phase Sample: 1. Diazepam • PCP
2. Nordiazepam-d5
3. Nordiazepam • THC
17619 4. Oxazepam-d5
2,3 4,5
5. Oxazepam
6. Temazepam-d5
7. Temazepam
6,7 9 8. Clonazepam
9. Lorazepam
10. Alprazoim ZB-Drug-1 Test Mix
11. a-Hydrozyalprozolam-d5 Part No.: AG0-8431
12. a-Hydrozyalprozolam

1
App ID 17619

Contact Phenomenex or your local

8 11,12 Phenomenex distributor for additional
GC products and applications.
10

4 4.5 5 5.5 6 6.5 7 min Extend column lifetime.

Add a Z-Guard™ to your next
Dimensions: 10 meter x 0.18 mm x 0.18 µm Zebron GC order.
Injection: Split 10:1 @ 250 °C, 1 µL
Carrier Gas: Helium @ 0.6 mL/min (constant flow)
Oven Program: 180 °C to 340 °C @ 20 °C/min for 2 min
Detector: MSD @ 320 °C

Ordering Information
Zebron ZB-Drug-1 GC Columns
ID(mm) df(µm) Temp. Limits °C Part No.
10-Meter
0.18 0.18 40 to 320/340 7CD-G023-08
15-Meter
0.25 0.25 40 to 320/340 7EG-G023-11
15-Meter with 5-Meter Guardian™ Integrated Guard
0.25 0.25 40 to 320/340 7EG-G023-11-GGA
30-Meter
0.25 0.25 40 to 320/340 7HG-G023-11
Note: If you need a 5 in. cage, contact technical support at www.phenomenex.com/chat or simply reach out to your
Technical consultant. Conditions may apply. Agilent 6850, some SRI and process GC systems use only 5 in. cages.

Phenomenex 129
 Zebron ™
GC Columns

ZB-BAC-1 and -2
Optimized Pair for Blood Alcohol Testing Upgrade to Zebron from these similar* phases:
• Enhanced accuracy for post mortem samples Agilent® Restek®
• DB®-ALC1 • Rtx®-BAC1
• Fast run time with baseline resolution of key components • DB-ALC2 • Rtx-BAC2
in just 2 minutes
*not exact equivalent, selectivity may differ
• Enhanced resolution of ethanol and acetone peaks
• Achieve confirmation with two elution order changes
when running columns in parallel
• Allows for the use of t-butanol or n-propanol as an internal
standard

Faster, More Sensitive Blood Alcohol Analysis

Zebron ZB-BAC-1 Zebron ZB-BAC-2
17982 x 0.53 mm x 3.00 µm
30 meter 30 meter
17983 x 0.53 mm x 2.00 µm
2 Run Blood Alcohol Analysis in 2
Under 5 min! 6
6

App ID 17983
5
App ID 17982

7 7
5 8 8
3 4 4
1 1 3
ZEBRON UNLIMITED GC COLUMNS | GC COLUMNS

1 2 3 4 min 1 2 3 4 min

Conditions for both columns:

Column: As listed Sample: Analytes 0.025 % and internal standards 0.100 % in water
Dimensions: As listed 1. Methanol
Part No.: 7HK-G021-36 (ZB-BAC-1) 2. Acetaldehyde
7HK-G022-32 (ZB-BAC-2) 3. Ethanol
Injection: Split 0.8:1 @ 150 °C, 1 mL 4. Isopropanol
Carrier Gas: Helium @ 80 cm/sec (constant flow) 5. Acetone
Oven Program: 40 °C (Isothermal) 6. t-Butanol (IS)
Detector: FID @ 250 °C 7. n-Propanol (IS)
8. 2-Butanol (IS)

Restek Rtx-BAC1 Restek Rtx-BAC2

30 meter x 0.53 mm x 3.00 µm 30 meter x 0.53 mm x 2.00 µm

4 Co-elution of t-Butanol 2
6
7 Internal Standard 6
2 5
3 5
1 4
8 3
1
7
App ID 18875

App ID 18876

0 1 2 3 4 min 0 1 2 3 4 5 6 7 min

Conditions for both columns:

Column: As listed Sample: Analytes and internal standards 0.100 % in water
Dimensions: As listed 1. Methanol 5. Acetone
Injection: Split 5:1 @ 150 °C, 1 mL 2. Acetaldehyde 6. t-Butanol (IS)
Carrier Gas: Helium @ 80 cm/sec (constant flow) 3. Ethanol 7. n-Propanol (IS)
Oven Program: 40 °C (Isothermal) 4. Isopropanol 8. 2-Butanol (IS)
Detector: FID @ 220 °C
Comparative separations may not be representative of all applications.

130 Phenomenex
Zebron ™
GC Columns

ZB-BAC-1 and -2 Column Profile

Run On Helium Or Hydrogen

18025
BAC-1
POLARITY* MS CERTIFIED
2

BAC-1
Column: Zebron ZB-BAC-1
Dimensions: 30 meter x 0.53 mm x 3.00 µm
Part No.: 7HK-G021-36 TEMP LIMITS ( °C )
-20 to 260/280

5
App ID 18025
*Similar polarity to ZB-35.
4
1 3 6

BAC-2
1 2 min 3 min POLARITY** MS CERTIFIED
18026

2
Column: Zebron ZB-BAC-2
BAC-2 Dimensions: 30 meter x 0.53 mm x 2.00 µm
Part No.: 7HK-G022-32 TEMP LIMITS ( °C )
-20 to 260/280

GC COLUMNS | ZEBRON UNLIMITED GC COLUMNS

App ID 18026

**Similar polarity to ZB-624.

4 Engineered Self Cross-linking™ (ESC)

3 6
1
Phase Chemistry

1 2 min 3 min • Proprietary

Conditions for both columns:
Injection: Split 5:1 @ 150 °C, 1 mL Recommended Applications
Carrier Gas: Hydrogen @ 80 cm/sec (constant flow)
Oven Program: 40 °C (Isothermal)
• Abused Inhalant Anesthetics
Detector: FID @ 250 °C
Sample: Analytes are 0.100 % in water • Blood Alcohol Analysis
1. Methanol
2. Acetaldehyde
3. Ethanol
4. Isopropanol
5. Acetone Contact Phenomenex or your local
6. n-Propanol Phenomenex distributor for additional
GC products and applications.
Ordering Information
Zebron ZB-BAC-1 GC Columns Extend column lifetime.
ID(mm) df(µm) Temp. Limits °C Part No. Add a Z-Guard™ to your next
30-Meter Zebron GC order.
0.32 1.80 -20 to 260/280 7HM-G021-31
0.53 3.00 -20 to 260/280 7HK-G021-36

Zebron ZB-BAC-2 GC Columns

ID(mm) df(µm) Temp. Limits °C Part No.
30-Meter
0.32 1.20 -20 to 260/280 7HM-G022-25
0.53 2.00 -20 to 260/280 7HK-G022-32
Note: If you need a 5 in. cage, contact technical support at www.phenomenex.com/chat or simply reach
out to your Technical consultant. Conditions may apply. Agilent 6850, some SRI and process GC systems
use only 5 in. cages.

Phenomenex 131
 Zebron ™
GC Columns

ZB-1plus™
MS Certified “1” Phase Upgrade to Zebron from any 100 % dimethylpolysiloxane phase:
Agilent® Restek® SGE® Supelco®
• DB®-1 • Rtx®-1 • BP1 • SPB®-1
• Very low bleed (MS Certified) phase especially suited to high • DB-1ms • Rtx-1ms • SolGel-1ms™ • SE-30
sensitivity GC-MS • DB-1ms Ultra Inert • Rxi®-1ms • MET-1
• HP-1 • MDN-1
• Extremely inert for active compounds such as drugs, • HP-1ms • Equity®-1
pesticides, or acids and bases • HP-1ms Ultra Inert
• VF-1ms
• Improved signal-to-noise ratio for better sensitivity and • CP-Sil 5 CB
• Ultra 1
mass spectral integrity
• Identical selectivity to 100 % dimethylpolysiloxane phases

Lower Overall Column Activity Lower Column Bleed

Activity is a key measure of column quality. ZB-1plus columns are We tested the ZB-1plus column bleed profile against other “MS”
aggressively tested to ensure full deactivation. Our QC test below columns on the market – ZB-1plus shows the lowest bleed, even at
demonstrates low tailing on ZB-1plus for even the most active com- temperatures up to 360 °C.
pounds, like 2-ethylhexanoic acid. ZB-1plus
TPGC Limit
360 ºC
17

ZB-1plus has lower bleed and higher Agilent

temperature limits! TPGC Limits
ZEBRON PLUS GC COLUMNS | GC COLUMNS

1 4 350 ºC
2

3
5
10
6 7 Zebron ZB-1PLUS
Agilent VF-1ms
8 Agilent HP-1ms
App ID 24538

1 5 10 min 0 15 30 min

Test conditions were stopped at 350 °C for all competitor columns so as not to cause damage to the
stationary phase by exceeding their maximum temperature limit.

Conditions for all columns:

Column: Zebron ZB-1plus Columns: As listed
Dimensions: 30 meter x 0.25 mm x 0.25 µm Dimensions: 30 meter x 0.25 mm x 0.25 µm
Part No.: 7HG-G031-11 Injection: Null Injection @ 250 ºC
Injection: Split 100:1 @ 250 °C, 1.0 µL Carrier Gas: Hydrogen @ 100 mL/min (constant flow)
Carrier Gas: Hydrogen @ 1.18 mL/min (constant flow) Oven Program: 240 °C for 9 min to 280 °C for 6.3 min to 320 °C for 6.4 min to 340 °C for
Oven Program: 140 °C (Isothermal) 5.8 min to 350 °C for 5.5 min to 360 °C
Detector: FID @ 325 °C Detector: FID @ 320 ºC
Sample: 1.  Decane
2.  2-Ethylhexanoic Acid
3.  4-Chlorophenol
4.  Naphthalene
5.  Tridecane
6.  1-Undecanol
7.  Dicyclohexylamine
8.  Pentadecane

Comparative separations may not be representative of all applications.

132 Phenomenex
Zebron ™
GC Columns

ZB-1plus™ Column Profile

Well-Suited for Food & Flavors POLARITY MS CERTIFIED

POLARITY MS CERTIFIED

Cold Pressed Orange Oil by GC-MS

3,4
55
1 6 7,8 13 USP PHASE TEMP LIMITS ( °C )
2 G1,USP
G2, PHASE
G9, G38 TEMP LIMITS ( °C )
-60 to 360/370
G1, G2, G9, G38 -60 to 360/370

9 14,15
16
*Thicker films (≥ 1.0 µm) are rated to 340/360 °C.

App ID 24527
5 20
10 12
17 18 19 21
22
*Thicker films
Engineered Self(≥Cross-linking
1.0 µm) are rated
™ to 340/360 °C.
(ESC)
11
Phase Chemistry

2 3 4 5 6 7 8 9 10 min CH3
Column:
Dimensions:
Zebron ZB-1plus
10 meter x 0.10 mm x 0.10 µm CH
Si 3 O
Part No.: 7CB-G031-02
Injection: Split 120:1 @ 160 °C, 0.2 µL
Carrier Gas: Helium @ 0.3 mL/min (constant flow) CH 3 O
Si
Oven Program: 60 °C to 130 °C @ 10 °C/min to 280 °C @ 30 °C/min for 3 min
Detector: MSD
Sample: Sample was 10 % in dichloromethane CH 3
100 % Dimethylpolysiloxane
1. α-Pinene 9. cis-Limonene oxide 17. α-Cubebene
Recommended Applications
2. β-Phellandrine 10. trans-Limonene oxide 18. β-Cubebene 100 % Dimethylpolysiloxane
3. β-Myrcene 11. Citronellal 19. Dodecanal
4. Octanal 12. α-Terpineol 20. Valencene • Acids
5. 3-Carene 13. Decanal 21. Cadinene
6. Limonene 14. Carvone 22. Nootkatone • Amines

GC COLUMNS | ZEBRON PLUS GC COLUMNS

7. Nonanal 15. Neral • Diesel Fuel
8. Linalool 16. Geranial
• Drugs
Ordering Information • EPA Methods (1668)
Zebron ZB-1plus GC Columns • Essential Oils
ID(mm) df(µm) Temp. Limits °C Part No.
5-Meter
• Flavors & Fragrances
0.18 0.18 -60 to 360/370 7AD-G031-08 • Oxygenates and GROs
10-Meter • PCBs
0.10 0.10 -60 to 360/370 7CB-G031-02
• Pesticides
12-Meter
0.20 0.33 -60 to 360/370 7DE-G031-14 • Solvent Impurities
15-Meter • Sulfur Compounds (Light)
0.25 0.25 -60 to 360/370 7EG-G031-11
0.32 0.25 -60 to 360/370 7EM-G031-11
15-Meter with 10-Meter Guardian™ Integrated Guard
0.25 0.25 -60 to 360/370 7EG-G031-11-GGC
20-Meter ZB-1plus Test Mix
0.18 0.18 -60 to 360/370 7FD-G031-08 Part No.: AG0-7805
25-Meter
0.20 0.33 -60 to 360/370 7GE-G031-14
30-Meter Contact Phenomenex or your local
0.25 0.10 -60 to 360/370 7HG-G031-02 Phenomenex distributor for additional
0.25 0.25 -60 to 360/370 7HG-G031-11 GC products and applications.
0.32 0.25 -60 to 360/370 7HM-G031-11
30-Meter with 5-Meter Guardian Integrated Guard
0.25 0.25 -60 to 360/370 7HG-G031-11-GGA Extend column lifetime.
Add a Z-Guard™ to your next
30-Meter with 10-Meter Guardian Integrated Guard
Zebron GC order.
0.25 0.25 -60 to 360/370 7HG-G031-11-GGC
60-Meter
0.25 0.25 -60 to 360/370 7KG-G031-11
0.25 1.00 -60 to 360/370 7KG-G031-22
0.32 0.25 -60 to 360/370 7KM-G031-11
Note: If you need a 5 in. cage, contact technical support at www.phenomenex.com/chat or simply reach
out to your Technical consultant. Conditions may apply. Agilent 6850, some SRI and process GC systems
use only 5 in. cages.

Phenomenex 133
 Zebron ™
GC Columns

ZB-5plus™
Inert 5 % Phenyl Selectivity Upgrade to Zebron from any
5 % phenyl / 95 % dimethylpolysiloxane phase:
Agilent® Restek® SGE® Supelco® OV®
• Highly inert—improved peak shape for acidic/basic • DB®-5 • Rtx®-5 • BP5 • MDN-5S • OV-5
compounds, drugs of abuse, and pesticides • HP-5 • Rtx-5MS • BPX5 • SPB®-5
• HP-5ms • Rtx-5Amine • Equity®-5
• Very low bleed (MS certified) levels provide maximum • HP-5msi • Rxi®-5ms
sensitivity
• Intense QC specifications ensure column-to-column
performance
19463
• ESC™ bonding results in phase stability and high Underivatized Antihistamines by GC-FID
temperature limits
5
• Traditional bonding chemistry provides the same selectivity
as the ZB-5 columns
4
8
Phenols 67
9

13,14,15
10
9,10,11
23

1 11
4,5 8 24 26

App ID 24540
12 16 27
3 17 20
18 19 21
6
12 25
App ID 24528

7 23
22

6 8 10 12 14 16 18 20 22 min 7.5 10 12.5 15 17.5 20 min

ZEBRON PLUS GC COLUMNS | GC COLUMNS

Column: Zebron ZB-5plus Column: Zebron ZB-5plus

Dimensions: 30 meter x 0.25 mm x 0.25 µm Dimensions: 30 meter x 0.25 mm x 1.0 µm
Part No.: 7HG-G032-11 Part No.: 7HG-G032-22
Injection: Split 5:1 @ 240 °C, 1 µL Injection: Split 50:1 @ 305 °C, 1 µL
Carrier Gas: Helium @ 1.2 mL/ min (constant flow) Carrier Gas: Helium @ 1.3 mL/min (constant flow)
Oven Program: 60 °C to 140 °C @ 5 °C/min to 280 °C @ 10 °C/min Oven Program: 40 °C for 1 min to 240 °C @ 25 °C/min for 2 min to 305 °C @
Detector: MSD @ 230 °C, 45-450 amu 25 °C/min for 8 min
Sample: 1. Phenol 16. 2,3-Dimethylphenol Detector: FID @ 320 °C
2. 2-Chlorophenol 17. 3,4-Dimethylphenol Sample: 1. Phenylpropanolamine 7. Phenyltoloxamine
3. 2-Methylphenol 18. 2,6-Dichlorophenol 2. Ephedrine 8. Methapyrilene
4. 4-Methylphenol 19. 4-Chloro-3-methylphenol 3. Pseudoephedrine 9. Chlorpheniramine
5. 3-Methylphenol 20. 2,4,6-Trichlorophenol 4. Pheniramine 10. Brompheniramine
6. 2,6-Dimethylphenol 21. 2,4,5-Trichlorophenol 5. Diphenhydramine 11. Triprolidine
7. 2-Nitrophenol 22. 2,4-Dinitrophenol 6. Doxylamine
8. 2-Ethylphenol 23. 4-Nitrophenol
9. 2,4-Dimethylphenol 24. 2,3,4,6-Tetrachlorophenol
10. 3,5-Dimethylphenol 25. 4,6-Dinitro-2-methylphenol
11. 2,5-Dimethylphenol 26. Pentachlorophenol
12. 4-Ethylphenol 27. Dinoseb
13. 3-Ethylphenol
14. 2,4-Dichlorophenol
15. Benzoic Acid

Endocrine Disruptors by GC-MS

16558 Column: Zebron ZB-5plus
15 21 Dimensions: 30 meter x 0.25 mm x 0.25 µm
8 Part No.: 7HG-G032-11
16 20 Injection: Split 40:1 @ 250 °C, 1 µL
Carrier Gas: Helium @ 1.2 mL/min (constant flow)
1 14 17 18 Oven Program: 100 °C to 180 °C @ 5 °C/min to 320 °C @ 15 °C/min
13 19 Detector: MSD @ 180 °C, 45-450 amu
12 Sample: Analytes are 50 ppm in acetone
2 1. Dimethyl phthalate 9. Terbuthylazine 17. 4,4’-DDD
7 9 22
11 2. Diethyl phthalate 10. Secbumeton 18. Di-n-hexyl phthalate
6 3. Atraton
5 10 11. Simetryn 19. 4,4’-DDT
4. Simazine 12. Ametryn 20. Dicyclohexyl phthalate
4
5. Prometon
App ID 24539

13. Prometryn 21. bis(2-Ethylhexyl)phthalate

3 23 6. Atrazine 14. Terbutryn 22. Di-n-octyl phthalate
7. Propazine 15. Dibutyl phthalate 23. Ethinyl estradiol
8. Dipropyl phthalate
16. 4,4’-DDE
11 12 13 14 15 16 17 18 19 20 21 22 23 24 min
134 Phenomenex
Zebron ™
GC Columns

ZB-5plus™ Column Profile

Good Results for Drugs

Drug Screening by GC-MS POLARITY MS CERTIFIED
16421 POLARITY MS CERTIFIED

8
1
22
29
8
25
21 26 28 USP PHASE TEMP LIMITS ( °C )
15 G27, G36, G41 -60 to 360/370
14 20 USP PHASE TEMP LIMITS ( °C )
16 19 G27, G36, G41 -60 to 360/370
8 13
17
9 12
3,4 10 23
56 11

App ID 24541
7 27
18
3031
24
32 Engineered Self Cross-linking™ (ESC)
2
Phase Chemistry
4 5 6 7 8 9 10 11 12 13 14 15 16 17 min

Column: Zebron ZB-5plus CH3

Dimensions: 30 meter x 0.25 mm x 0.25 µm
Part No.: 7HG-G032-11
Injection: Split 15:1 @ 240 °C, 1 µL Si O SiCHO
3
Carrier Gas: Helium @ 1.1 mL/min (constant flow)
Oven Program: 140 °C to 240 °C @ 10 °C/min for 5 min to 320 °C @
25 °C/min for 2.25 min Si O CH
Si 3 O
Detector: MSD @ 230 °C, 45-450 amu
Sample: Analytes (underivatized) are 25 ppm in dichloromethane 5 % Phenyl 95 % Dimethylpolysiloxane
1. Nicotine
2. Methylecgonine
14. Caffeine
15. Benzphetamine
27. Morphine
28. Diazepam Recommended Applications
CH 3
3. Ibuprofen 16. Hexobarbital 29. Hydrocodone 5 % Phenyl 95 % Dimethylpolysiloxane
4. Allobarbital 17. Dimenhydrinate 30. 6-Monoacetylmorphine
5. Aprobarbital 18. Doxylamine 31. Oxymorphone
• Barbiturates
6. Butabarbital 19. Phenobarbital 32. Diacetylmorphine (Heroin) • Benzodiazepines

GC COLUMNS | ZEBRON PLUS GC COLUMNS

7. Acetaminophenl 20. 8-Chlorotheophylline
8. Phenacetin 21. Methapyrilene • Drugs of Abuse
9. Amobarbital 22. Chlorpheniramine • EPA Methods
10. Pentobarbital 23. Brompheniramine
11. Secobarbital 24. Cocaine • FAMEs
12. Meprobamate 25. Chlorcyclizine
13. Methyl benzilate 26. Codeine • Nitrosamines
• Pesticides
• Phenols
Ordering Information
Zebron ZB-5plus GC Columns • THC Metabolites
ID(mm) df(µm) Temp. Limits °C Part No.
10-Meter
0.18 0.18 -60 to 360/370 7CD-G032-08
15-Meter
0.25 0.25 -60 to 360/370 7EG-G032-11 ZB-5plus Test Mix
20-Meter Part No.: AG0-8362
0.18 0.18 -60 to 360/370 7FD-G032-08
0.18 0.36 -60 to 360/370 7FD-G032-53
20-Meter with 5-Meter Guardian™ Integrated Guard
For high temperature analysis,
0.18 0.18 -60 to 360/370 7FD-G032-08-GGA
consider using a ZB-5HT, see p. 146
30-Meter
0.25 0.25 -60 to 360/370 7HG-G032-11
0.25 0.50 -60 to 360/370 7HG-G032-17
Contact Phenomenex or your local
0.25 1.00 -60 to 360/370 7HG-G032-22 Phenomenex distributor for additional
0.32 0.25 -60 to 360/370 7HM-G032-11 GC products and applications.
0.32 0.50 -60 to 360/370 7HM-G032-17
0.32 1.00 -60 to 360/370 7HM-G032-22
30-Meter with 5-Meter Guardian Integrated Guard Extend column lifetime.
Add a Z-Guard™ to your next
0.25 0.10 -60 to 360/370 7HG-G032-02-GGA
Zebron GC order.
0.25 0.25 -60 to 360/370 7HG-G032-11-GGA
60-Meter
0.25 0.25 -60 to 360/370 7KG-G032-11
60-Meter with 5-Meter Guardian Integrated Guard
0.25 0.25 -60 to 360/370 7KG-G032-11-GGA
Note: If you need a 5 in. cage, contact technical support at www.phenomenex.com/chat or simply
reach out to your Technical consultant. Conditions may apply. Agilent 6850, some SRI and process
GC systems use only 5 in. cages.

Phenomenex 135
 Zebron ™
GC Columns

ZB-5MSplus™

The Next Generation of Inertness Upgrade to Zebron from any

5 % phenyl or 5 % phenyl-arylene / 95 % dimethylpolysiloxane phase:
Agilent® Restek® Supelco®
• The next generation of inertness for specialty chemical, • DB®-5ms • Rxi®-5Sil MS • SLB®-5ms
forensic, toxicology, and food testing applications • DB-5ms Ultra Inert
• HP-5ms
• Specialized deactivation for versatile 5 % phenyl-arylene • HP-5ms Ultra Inert
selectivity with improved sensitivity • VF-5ms

• Low bleed (MS Certified) and well-suited to high sensitivity

GC-MS and GC-MS/MS work

Engineered for High Performance

Active sites on a GC column’s surface can result in analyte ad-
sorption and degradation, negatively affecting peak shape and
response. To reduce potential surface activity, Zebron ZB-5MSplus
is designed with a rigorous fused silica deactivation process that
improves inertness for troublesome compounds. Instantly achieve
higher responses for active compounds compared to your current
5ms phase column, without changing your selectivity.

Melamine in Dog Food by GC-MS

23244
ZEBRON PLUS GC COLUMNS | GC COLUMNS

3 5
Low bleed 5 %
phenyl-arylene
2 phase chemistry
for versatile
applications
App ID 23244

Clean, highly inert

fused silica surface
due to very few
active sites
9 10 11 12 13 14 min

Extraction Protocol: 1. Combine 0.5 g of homogenized dog food with 10 mL of DEA/Water/
Acetonitrile (1:4:5) in a 15 mL centrifuge tube
2. Sonicate for 30 min
3. Centrifuge at 5000 rpm for 10 min
4. Transfer 100 µL of supernatant to an autosampler vial and evaporate
to dryness using nitrogen gas
5. Reconstitute with 100 µL of Acetonitrile/Pyridine (1:1) and then
derivatize using 100 µL BSTFA with 1 % TCMS at 70 °C for 45 min

Column: Zebron ZB-5MSplus

Dimensions: 30 meter x 0.25 mm x 0.25 µm
Part No.: 7HG-G030-11
Injection: Splitless @ 280 °C, 1 µL
Carrier Gas: Helium @ 1 mL/min (constant flow)
Oven Program: 75 °C for 1 min to 320 °C @ 15 °C/min hold for 4 min
Detector: MSD @ 320 °C
Sample: 1. Cyanuric acid
2. Ammelide
3. Ammeline
4. Melamine
5. Benzoguanamine

136 Phenomenex
 Zebron ™
GC Columns

ZB-5MSplus™ Column Profile

Versatile Performance For Drugs and Chemicals

23229 POLARITY MS CERTIFIED
Cannabinoids In Brownies 3
by GC-MS POLARITY MS CERTIFIED
8
1
USP PHASE
8
TEMP LIMITS ( °C )
Good peak shape G27 -60 to 325/350
from extracted matrix 2
USP PHASE TEMP LIMITS ( °C )
G27 -60 to 325/350

App ID 23229 Engineered Self Cross-linking™ (ESC)

5.25 5.50 5.75 6.00 6.25 6.50 6.75 min
Phase Chemistry
Extraction Protocol: Column: Zebron ZB-5MSplus
1. Combine 1 g of chocolate brownie with 10 mL of water in Dimensions: 30 meter x 0.25 mm x 0.25 µm
a 50 mL centrifuge tube Part No.: 7HG-G030-11 5 % Phenyl-Arylene
Injection: Splitless @ 250 °C, 1 µL 5 % Phenyl-Arylene
2. Shake using a mechanical shaker until dissolved
3. Add roQ™ QuEChERS EN15662 extraction salt packet Carrier Gas: Helium @ 1.5 mL/min (constant flow) CH3 CH3 CH3
(KS0-8909) and 10 mL of acetonitrile Oven Program: 100 °C for 1 min to 320 °C @ 50 °C/min, CH3 CH3 CH3
4. Shake tube for 3 min using mechanical shaker hold for 2 min Si Si O Si O
5. Centrifuge at 2700 rpm for 5 min Detector: MSD @ 320 °C
6. Transfer 1 mL of supernatant to an autosampler vial for Sample: 1. Cannabidiol Si CH SiCHO Si
CH O
23246 GC-MS analysis 2. ∆-9-Tetrahydrocannabinol 3 3 3
3. Cannabinol 95 % Dimethylpolysiloxane
Ethanolamines
3
CH 3 CH 3 CH 3
Recommended Applications
by GC-MS 95 % Dimethylpolysiloxane
• Acids
Awesome • Alkaloids

GC COLUMNS | ZEBRON PLUS GC COLUMNS

peak shape!
• Amines
App ID 23246

1 2 4
• Drugs
• Essential Oils
• Flavors
2 3 4 5 6 min
• Halo-hydrocarbons
Column: Zebron ZB-5MSplus Oven Program: 30 °C to 300 °C @ 40 °C/min
Dimensions: 30 meter x 0.25 mm x 1.00 µm Detector: MSD @ 320 °C • Pesticides
Part No.: 7HG-G030-22 Sample: 1. Monoethanolamine • Phenols
Injection: Split 200:1 @ 250 °C, 1 µL 2. Diethanolamine
Carrier Gas: Helium @ 1.4 mL/min (constant flow) 3. Triethylene glycol monomethyl ether (IS) • Residual Solvents
4. Triethanolamine
• Solvent Impurities
Ordering Information
Zebron ZB-5MSplus GC Columns
ID(mm) df(µm) Temp. Limits °C Part No. Contact Phenomenex or your local
1.5-Meter Phenomenex distributor for additional
0.25 0.25 -60 to 325/350 7XG-G030-11 GC products and applications.
15-Meter
0.25 0.25 -60 to 325/350 7EG-G030-11 Extend column lifetime.
Add a Z-Guard™ to your next
0.25 0.50 -60 to 325/350 7EG-G030-17
Zebron GC order.
0.25 1.00 -60 to 325/350 7EG-G030-22
20-Meter Ordering Information
0.18 0.18 -60 to 325/350 7FD-G030-08 Zebron ZB-5MSplus GC Columns (cont’d)
0.18 0.36 -60 to 325/350 7FD-G030-53 ID(mm) df(µm) Temp. Limits °C Part No.
30-Meter 30-Meter with 5-Meter Guardian™ Integrated Guard
0.25 0.25 -60 to 325/350 7HG-G030-11 0.25 0.25 -60 to 325/350 7HG-G030-11-GGA
0.25 0.50 -60 to 325/350 7HG-G030-17 0.25 0.50 -60 to 325/350 7HG-G030-17-GGA
0.25 1.00 -60 to 325/350 7HG-G030-22 30-Meter with 10-Meter Guardian Integrated Guard
0.32 0.25 -60 to 325/350 7HM-G030-11 0.25 0.25 -60 to 325/350 7HG-G030-11-GGC
0.32 0.50 -60 to 325/350 7HM-G030-17 0.25 0.50 -60 to 325/350 7HG-G030-17-GGC
0.32 1.00 -60 to 325/350 7HM-G030-22 60-Meter
0.32 1.50 -60 to 325/350 7HM-G030-28 0.25 0.25 -60 to 325/350 7KG-G030-11
0.53 1.00 -60 to 325/350 7HK-G030-22 0.25 1.00 -60 to 325/350 7KG-G030-22
0.53 3.00 -60 to 325/350 7HG-G030-36 0.32 1.00 -60 to 325/350 7KM-G030-22
Note: If you need a 5 in. cage, contact technical support at www.phenomenex.com/chat or simply reach
out to your Technical consultant. Conditions may apply. Agilent 6850, some SRI and process GC systems
use only 5 in. cages.

Phenomenex 137
 Zebron ™
GC Columns

ZB-WAXplus™

Enhanced Aqueous Stability Upgrade to Zebron from any polyethylene glycol phase:
• 100 % aqueous stable, excellent for aqueous samples Agilent® Restek® SGE® Supelco®
• DB®-WAX • Stabilwax® • BP20 • SUPELCOWAX® 10
• Extremely inert for acidic compounds • CAM
• HP-20M
• Enhanced selectivity for low boiling solvents • Carbowax 20M
• High retention of alcohols and chlorinated solvents • CP-Wax 52 CB

• Increased efficiency at 20 °C

Water Reproducibility of ZB-WAXplus

Historically, polyethylene glycol (PEG) phases have been unstable
with aqueous samples such as beverages or glycols, resulting in
poor reproducibility and decreased lifetime. ZB-WAXplus bonding
procedure results in exceptional stability to repeated injections of
aqueous matrices.

Scottish Single Malt Whiskey Column: Zebron ZB-WAXplus

60 % Aqueous! Dimensions: 30 meter x 0.25 mm x 0.25 µm
4 7,8 Part No.: 7HG-G013-11
Injection: Split 30:1 @ 140 °C, 0.2 µL
pA Carrier Gas: Helium @ 1.4 mL/min (constant flow)
90
Oven Program: 35 ˚C for 5 min to 85 ˚C @ 10 ˚C/min to 200 ˚C @
80
App ID 15817

70
25 ˚C/min for 1 min
60 Detector: FID @ 200 °C
6
Sample: 1. Acetaldehyde
ZEBRON PLUS GC COLUMNS | GC COLUMNS

50
5
40 23
2. Ethyl Acetate
1
30 3. Methanol
20 4. Ethanol
10 5. Propanol
0
0 2 4 6 8 10 12 14 m in 6. Isobutanol
7. 2-Methylbutanol
8. 3-Methylbutanol

Improve Resolution

Better
Resolution
Zebron ZB-WAXplus Restek Stabilwax
15 17 8,9 15
8 17
13 14 11 13 14
11
Coelution

2
16 4 16
4 6
6 2 10
10 12
12 3
3 7
App ID 15816
App ID 15815

7 9
1
1

5 5

2 3 4 5 6 7 min 2 3 4 5 6 7 min

Conditions same for both columns:

Dimensions: 30 meter x 0.25 mm x 0.25 µm Sample: 1. Methyl Formate 10. 2-Butanol
Injection: Split 100:1 @ 250 °C, 1 μL 2. Acetone 11. Toluene
Carrier Gas: Hydrogen @ 1.0 mL/min (constant flow) 3. Ethyl Acetate 12. n-Propanol
Oven Program: 35 °C for 2.5 min to 85 °C @ 10 °C/min and hold until last peak elutes 4. Methyl Ethyl Ketone 13. Ethyl Benzene
Detector: FID @ 225 °C 5. Methanol 14. p-Xylene
6. 2-Methyl-2-propanol 15. m-Xylene
7. Methylene Chloride 16. 1-Butanol
8. Benzene 17. o-Xylene
Comparative separations may not be representative of all applications. 9. Ethanol

138 Phenomenex
Zebron ™
GC Columns

ZB-WAXplus™ Column Profile

A Food Testing Must-Have POLARITY

POLARITY

Food Industry FAMEs

16321 52
52
USP PHASE
USP PHASE TEMP LIMITS ( °C()°C )
TEMP LIMITS
2 3 4 G14, G15, G16, 20 to 250/260*
6 G14, G15, G16, 20 to 250/260*
G20, G39, G47
G20, G39, G47
1

5 8 *Thicker films (≥ 1.0 µm) are rated to 230/240 °C.

12
*Thicker films (≥ 1.0 µm) are rated to 230/240 °C.

Phase Chemistry
7

9 16
17,18 H H
23
10
11
13 27
31 35
O CH CH O
20 26 28
App ID 16321

14 15 36
19 21 22 24
25
29 33
30 32 34 37 O HC HC O
H H
100 % Polyethylene Glycol

10 20 30 40 min Recommended Applications

100 % Polyethylene Glycol
Column: Zebron ZB-WAXplus
Dimensions: 30 meter x 0.25 mm x 0.25 µm • Alcohols

GC COLUMNS | ZEBRON PLUS GC COLUMNS

Part No.: 7HG-G013-11 • Aldehydes
Injection: Split 5:1 @ 220 °C, 1 µL
Carrier Gas: Helium @ 3 mL/min (constant flow) • Aromatics
Oven Program: 60 °C for 2 min to 150 °C @ 13 °C/min to 240 °C @ 2 °C/min
Detector: FID @ 250 °C
• Essential Oils
Sample: See the full compound list at www.phenomenex.com/GC • Flavors & Fragrances
• Free Fatty Acids
• Glycols
Ordering Information • OVIs
Zebron ZB-WAXPLUS GC Columns
• Pharmaceuticals
ID(mm) df(µm) Temp. Limits °C Part No.
10-Meter • Solvents / Residual Solvents
0.10 0.10 20 to 250/260 7CB-G013-02 • Styrene
15-Meter • Xylene Isomers
0.25 0.25 20 to 250/260 7EG-G013-11
0.53 1.00 20 to 230/240 7EK-G013-22
20-Meter
0.18 0.18 20 to 250/260 7FD-G013-08
ZB-WAXplus Test Mix
30-Meter
0.25 0.25 20 to 250/260 7HG-G013-11 Part No.: AG0-7869
0.25 0.50 20 to 250/260 7HG-G013-17
0.32 0.25 20 to 250/260 7HM-G013-11
0.32 0.50 20 to 250/260 7HM-G013-17 Contact Phenomenex or your local
0.32 1.00 20 to 230/240 7HM-G013-22 Phenomenex distributor for additional
0.53 0.25 20 to 250/260 7HK-G013-11 GC products and applications.
0.53 1.00 20 to 230/240 7HK-G013-22
60-Meter Extend column lifetime.
0.25 0.15 20 to 250/260 7KG-G013-05 Add a Z-Guard™ to your next
0.25 0.25 20 to 250/260 7KG-G013-11 Zebron GC order.
0.25 0.50 20 to 250/260 7KG-G013-17
0.32 0.25 20 to 250/260 7KM-G013-11
0.32 0.50 20 to 250/260 7KM-G013-17
0.53 1.00 20 to 230/240 7KK-G013-22
Note: If you need a 5 in. cage, contact technical support at www.phenomenex.com/chat or simply reach
out to your Technical consultant. Conditions may apply. Agilent 6850, some SRI and process GC systems
use only 5 in. cages.

Phenomenex 139
 Zebron ™
GC Columns

ZB-624plus™
• Enhanced peak shape with superior deactivation
• Increased sensitivity for high boiling solvents
Upgrade to Zebron from these similar* phases:
• Extremely low bleed for GC-MS
Agilent® Restek®
• High temperature stability (300/320 ºC) • CP-Select 624 CB • Rxi®-624Sil MS
• DB-624UI Ultra Inert
*not exact equivalent, selectivity may differ

What Makes the PLUS in ZB-624plus? Shorter Residual Solvent Analysis

by GC-FID
• Enhanced Inertness
Proprietary superior deactivation gives great peak shape for Why wait for an hour long method. Upgrade to ZB-624plus and get
troublesome compounds.
short runtime, low bleed, high temperature resistance, and the 624
• High Selectivity selectivity, all in one column.
A G43 phase that’s highly selective for polar, non-polar, low and
high boiling solvents.
Separation24776
of Residual Solvent Critical Pairs in Less than 15 min
• Column-to-Column Reproducibility
Excellent column-to-column reproducibility well suited for 800
7
validated methods. 4
700 3
• Temperature Limits 600 2
Push the temperature limits of traditional 624 and elute/bake
high boiling analytes at 300/320 °C. 500 9
• MS Certified 400
pA

Low bleed characteristics makes it the right choice for GC-MS.

App ID 24776
300
1
200 8

100 5 6

4 5 6 7 8 9 10 min
ZEBRON PLUS GC COLUMNS | GC COLUMNS

Column: Zebron ZB-624plus

Dimensions: 30 meter x 0.32 mm x 1.80 μm
Part No.: 7HM-G040-31
Injection: Split 20:1 @ 200 °C, 1 μL
Recommended Liner: Zebron PLUS Straight Z-Liner™
Liner Part No.: AG2-0A03-05 (for Agilent® & Thermo Scientific® systems)
Carrier Gas: Helium @ 1 mL/min (constant flow)
Oven Program: 40 °C for 5 min to 260 °C @ 25 °C/min for 3 min
Detector: FID @ 250 °C
Sample: 1. Methanol 6. DCM
2. Ethanol 7. Ethyl Acetate
3. Acetone 8. THF
4. IPA 9. Toluene
5. Acetonitrile

Superior Deactivated Fused Silica

Dramatically reduces analyte adsorption, maximizing
your peak symmetry.

Highly Selective Stationary Phase

Provides excellent separation of polar, nonpolar, low and
high boiling solvents, while Engineered Self Cross-linking
(ESC™) results in high-thermal stability and low bleed.

Polyimide Coating
Flexibility and temperature resistance
(300/320°C).
0.53 mm ID ZB-624plus™ columns are not MS Certified.

140 Phenomenex
Zebron ™
GC Columns

ZB-624plus™ 2019 PittCon

Excellence Award Column Profile

We QC Test for the Compounds You Analyze

POLARITY MS CERTIFIED
We added challenging and troublesome analytes to our QC test
to make sure each ZB-624plus column has superior deactivation.
13
USP PHASE TEMP LIMITS ( °C )
G43 -20 to 300/320

24805-overlay

Consistent Retention, Sensitivity, and

1 Peak Shape from Column-to-Column

Engineered Self Cross-linking™ (ESC)

4 Phase Chemistry
2
3
5
• Proprietary
6
Recommended Applications
7
8
• Cannabis
• Terpenes
• Residual Solvents
Column 1 • Volatile Amines
• EPA Method 8260
• EPA Method 524

GC COLUMNS | ZEBRON PLUS GC COLUMNS

Column 2 • EPA Method 624
• Food
App ID 24805

• Flavors and Fragrances

Column 3
• Solvent Purity
2 3 4 5 6 7 8 9 10 min • Alcohols

Test Probe The Plus Advantage Property

2,4-Dimethylphenol We screen challenging analytes, like acids and
Inertness ZB-624plus Test Mix
2,4-Dimethylaniline bases, to mimic your most challenging compounds.
Part No.: AG0-9203

Conditions for all separations:

Column: Zebron ZB-624plus
Dimensions: 30 meter x 0.32 mm x 1.80 μm Contact Phenomenex or your local
Part No.: 7HM-G040-31 Phenomenex distributor for additional
Injection: Split 50:1 @ 250 °C, 1 μL GC products and applications.
Recommended Liner: Zebron PLUS Straight Z-Liner™
Liner Part No.: AG2-0A03-05 (for Agilent® & Thermo Scientific® systems)
Carrier Gas: Hydrogen @ 6 psi (constant pressure)
Oven Program: 85 °C for 10.5 min
Detector: FID @ 305 °C
Sample: 1. Methane
2. Dodecane
3. 2,4-Dimethylphenol
4. 2,4-Dimethylaniline
5. Tridecane
6. 1-Methylnaphthalene
7. 1-Undecanol
8. Pentadecane

Phenomenex 141
 Zebron ™
GC Columns

ZB-624plus™ (cont’d)
Improved Peak Shape of Volatile Amines
Volatile amines are challenging analytes for GC analysis. They can
adsorb to even the smallest imperfections in fused silica. ZB‑624plus
undergoes a superior deactivation process which minimizes active
compound adsorption leading to gains in peak response and shape.

Comparison of Volatile Amines on Various 624 Columns

24778-82-83

260 2 Better deactivation = Higher

sensitivity for active analytes
240
3
1
220
Volatile Amines on a Zebron ZB-624plus - 500 ppm
200
Volatile Amines on a Restek® Rxi®-624Sil MS - 500 ppm
180
Volatile Amines on a Agilent® DB®-624UI Ultra Inert - 500 ppm
160

140 Conditions for all separations:

Volatile amine Column: Zebron ZB-624plus
pA

120 adsorption resulting Restek Rxi-624Sil MS

in poor peak symmetry Agilent DB-624UI Ultra Inert
100 Dimensions: 30 meter x 0.32 mm x 1.80 μm
80 Injection: Split 20:1 @ 200 °C, 1 μL
Recommended Liner: Zebron PLUS Straight Z-Liner™
60 Liner Part No.: AG2-0A03-05 (for Agilent® & Thermo Scientific® systems)
App ID 24778
App ID 24783
App ID 24782

40
Carrier Gas: Helium @ 1.8 mL/min (constant flow)
Oven Program: 50 °C for 1 min, to 200 °C @ 20 °C/min for 5 min
20 Detector: FID @ 250 °C
ZEBRON PLUS GC COLUMNS | GC COLUMNS

Sample: 1. Isopropylamine
2.0 2.5 3.0 3.5 4.0 4.5 5.0 min 2. Diethylamine
3. Triethylamine

Volatile Organic Compounds in EPA Methods 8260, 524, and 624

Our high efficiency dimension and superior deactivation can stand
real world samples. In addition, MS certification provides extreme
low bleed to your GC-MS analysis.

GC-MS Analysis of 74 volatile organic

24790
compounds in less than 15 min
Column: Zebron ZB-624plus
1.0E5 Dimensions: 30 meter x 0.25 mm x 1.40 µm
Part No.: 7HG-G040-27
9.0E4 Injection: Split 50:1 @ 230°C, 1 µL
Recommended Liner: Zebron PLUS Straight Z-Liner™
8.0E4
Liner Part No.: AG2-0A03-05 (for Agilent® & Thermo Scientific® systems)
7.0E4 Carrier Gas: Helium @ 0.7 mL/min (constant flow)
Oven Program: 40 °C for 2 min, to 210 °C @ 17 °C/min for 3 min
Abundance

6.0E4
Detection: Mass Spec transfer line @ 250 °C
5.0E4

4.0E4
App ID 24790

3.0E4
Low bleed
2.0E4

1.0E4

0.0E0
2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 14.0 min

Comparative separations may not be representative of all applications.

142 Phenomenex
Zebron ™
GC Columns

ZB-624plus™ (cont’d)
Exceeding USP <467> System Suitability
USP <467> method requires resolution of 1.5 for critical pairs.
Zebron ZB-624plus took the challenge and succeeded with
even greater resolution!

Class 124772
Residual Solvents Class 2B Residual Solvents
Peak Resolution = 4.0 24774
900 1 Good Peak Shape
160 for Pyridine 8
800
700 Peak Resolution = 2.0 140
600
120
500
pA

2 4

pA
400 100 6
300
5 Baseline Resolution Between
80 4
App ID 24772

App ID 24774
Critical Pairs 3 7
200 1 2
3 60 5
100
40
5 10 15 20 25 min 5 10 15 20 25 30 35 min
Sample: 1. 1,1-Dichloroethene 4. Benzene Sample: 1. Hexane 5. Trichlorethylene
2. 1,1,1-Tetrachloride 5. 1,2-Dichloroethane 2. Nitromethane 6. Pyridine
3. Carbon tetrachloride 3. Chloroform 7. 2-Hexanone
4. 1,2-Dimethoxyethane 8. Tetralin

Class 2A24773
Residual Solvents Class 1 24775
and 2A Residual Solvents
1 Peak Resolution = 2.4
4000 1600 1 Peak Resolution = 3.1

GC COLUMNS | ZEBRON PLUS GC COLUMNS

3500 1400
Peak Resolution = 3.0
3000 13 1200 11
2500 1000
pA

16
pA

2000 800 Peak Resolution = 2.2

1500 7
600
App ID 24773

8 10

App ID 24775
10 12 14 15
1000 2 11 400 14 17
500 4 56 8 23 13
3 9 200 45 67 12
9
0 0
5 10 15 20 25 min 5 10 15 20 25 min
Sample: 1. Methanol 8. Methyl cyclohexane Sample: 1. Methanol 10. Benzene
2. Acetonitrile 9. 1,4-Dioxane 2. 1,1-Dichloroethene 11. 1,2-Dichloroethane
3. Dichloromethane 10. Toluene 3. Acetonitrile 12. 1,4-Dioxane
4. trans-1,2-Dichloroethane 11. Chlorobenzene 4. Dichloromethane 13. Toluene
5. cis-1,2-Dichloroethane 12. Ethyl benzene 5. trans-1,2-Dichloroethene 14. Chlorobenzene
6. Tetrahydrofuran 13. m-Xylene / p-Xylene 6. cis-1,2-Dichloroethene 15. Ethyl benzene
7. Cyclohexane 14. o-Xylene 7. Tetrahydrofuran 16. m-Xylene/ p-Xylene
8. 1,1,1-Tetrachloride 17. o-Xylene
9. Carbon tetrachloride
Ordering Information
Same conditions for all separations: Zebron ZB-624plus GC Columns
Column: Zebron ZB-624plus ID(mm) df(µm) Temp. Limits °C Part No.
Dimensions: 30 meter x 0.32 mm x 1.80 µm 20-Meter
Part No.: 7HM-G040-31 0.18 1.00 -20 to 300/320 7FD-G040-22
Injection: Split 5:1 @ 140 °C, 1 µL 0.25 1.40 -20 to 300/320 7FG-G040-27
Recommended Liner: Zebron PLUS Straight Z-Liner™ 30-Meter
Liner Part No.: AG2-0A03-05 (for Agilent® & Thermo Scientific® systems)
0.25 1.40 -20 to 300/320 7HG-G040-27
Carrier Gas: Helium @ 2.2 mL/min (constant flow)
0.32 1.80 -20 to 300/320 7HM-G040-31
Oven Program: 40 °C for 20 min to 240 °C @ 10 °C/min
Detector: FID @ 250 °C 0.53 3.00 -20 to 300/320 7HK-G040-36
60-Meter
0.25 1.40 -20 to 300/320 7KG-G040-27
0.32 1.80 -20 to 300/320 7KM-G040-31
0.53 3.00 -20 to 300/320 7KK-G040-36
75-Meter
0.53 3.00 -20 to 300/320 7LK-G040-36
Note: If you need a 5 in. cage, contact technical support at www.phenomenex.com/chat or simply reach
out to your Technical consultant. Conditions may apply. Agilent 6850, some SRI and process GC systems
use only 5 in. cages. 0.18 mm, 0.25 mm, and 0.32 mm IDs are MS certified.

Phenomenex 143
 Zebron ™
GC Columns

ZB-1HT Inferno™ Upgrade to Zebron from any

100 % dimethylpolysiloxane phase:
Agilent® Restek® SGE® Supelco®
Robust Results Up to 430 ºC • DB®-1 • Rtx®-1 • BP1 • SPB®-1
• DB-1ht • Rxi®-1HT • Petrocol® 2887
• HP-1
• CP-Sil 5 CB
• First non-metal columns stable to 430 °C • CP-SimDist
• Provides true boiling point separation for hydrocarbon
distillation methods
• Longer lifetime with rugged high temperature, polyimide
coated, fused silica tubing
• Low activity, provides good peak shape for acidic and
basic samples
• Provides robust column performance for high
temperature bake outs

Rugged, High-Temperature Performance

Great
16148 Separation of High Boiling Hydrocarbons (ASTM D6352)

Zebron’s ZB-1HT Inferno can Column: Zebron ZB-1HT Inferno

33 operate at 430 °C, allowing C90 Dimensions: 5 meter x 0.53 mm x 0.10 μm
6 7 9 31 Part No.: 7AK-G014-02
5 8 10 12 to elute on temperature ramp to
11 1314
Injection: On-Column @ 43 °C, 0.1 μL
4 27 29 meet ASTM requirements
1516 18 Carrier Gas: Helium @ 4.4 mL/min (constant flow)
17 19 21 23
25 Oven Program: 40 °C for 0.5 min to 430 °C @ 20 °C/min for 10 min
22
20 24
3 Detector: FID @ 430 °C
26 3738
ZEBRON INFERNO GC COLUMNS | GC COLUMNS

2830 36 39 41 Sample: 1. C10 16. C23 31. C38 46. C66

32
2 35 40 42 2. C11 17. C24 32. C39 47. C68
43 3. C12 18. C25
44 33. C40 48. C70
34 45 4. C13 19. C26 34. C42 49. C72
1 46 5. C14 20. C27 35. C44 50. C74
47 6. C15 21. C28 36. C46 51. C76
48
49 56 58 7. C16 22. C29 37. C48 52. C78
50 5254 57 8. C17 23. C30 38. C50 53. C80
51 55
App ID 16148

53 9. Pristane 24. C31 39. C52 54. C82

10. C18 25. C32 40. C54 55. C84
11. Phytane 26. C33 41. C56 56. C86
12. C19 27. C34 42. C58 57. C88
13. C20 28. C35 43. C60 58. C90
0 5 10 15 20 min 14. C21 29. C36 44. C62
15. C22 30. C37 45. C64
Note: Sample was a combination of PolyWax® 655
and retention time markers C8-C40 in CS2/Chloroform

16071
Bake Off Contaminants from Dirty Matrices

Column: Zebron ZB-1HT Inferno

Dimensions: 30 meter x 0.25 mm x 0.10 µm
Part No.: 7HG-G014-02
Injection: On-Column @ 153 °C, 1 µL
Carrier Gas: Helium @ 1 mL/min (constant flow)
Oven Program: 150 °C to 400 °C @ 14 °C/min for 6 min
Synthetic Motor Oil Detector: FID @ 400 °C
Sample: Sample was 1 % in dichloromethane
Mobil® 1 10W-30 Fully Synthetic Motor Oil
App ID 16071

0 5 10 15 20 min

144 Phenomenex
Zebron ™
GC Columns
2007 R&D 100
Award Recipient
ZB-1HT Inferno ™ Column Profile

Run Versatile Samples

POLARITY MS CERTIFIED

POLARITY MS CERTIFIED
Hydrocarbons
18153
from Water by GC-FID 5
DIN EN ISO 9377-2 (DEV H53)
5
USP PHASE TEMP LIMITS ( °C )
G1, G2, G9, G38 -60 to 400/430 *
USP PHASE TEMP LIMITS ( °C )
G1, G2, G9, G38 -60 to 400/430 *
1

*0.53 mm ID columns are rated to 400 °C.

*0.53 mm ID columns are rated to 400 °C.
Engineered Self Cross-linking™ (ESC)

Phase Chemistry

CH
CH3 3
2
Si O
Si O
App ID 18153

CH 3
3
CH
100 % Dimethylpolysiloxane
0 2 4 6 8 10 12 14 min
100 % Dimethylpolysiloxane
Recommended Applications

GC COLUMNS | ZEBRON INFERNO GC COLUMNS

Column: Zebron ZB-1HT Inferno
Dimensions: 15 meter x 0.32 mm x 0.25 μm • Diesel Fuel
Part No.: 7EM-G014-11
Injection: Splitless @ 300 °C, 20 μL
• High Boiling Petroleum Products
Carrier Gas: Helium @ 2.0 mL/min (constant flow) • High Molecular Weight Waxes
Oven Program: 50 °C for 2 min to 320 °C @ 30 °C/min for 5 min
Detector: FID @ 330 °C • Hydrocarbons
Sample: 1. Decane (C10) • Motor Oils
2. Tetracontane (C40)
• Polymers/Plastics
• Simulated Distillation
Ordering Information
Zebron ZB-1HT Inferno GC Columns
ID(mm) df(µm) Temp. Limits °C Part No.
5-Meter
0.53 0.10 -60 to 400 7AK-G014-02 ZB-1HT Test Mix
10-Meter Part No.: AG0-5155
0.32 0.25 -60 to 400/430 7CM-G014-11
15-Meter
0.25 0.10 -60 to 400/430 7EG-G014-02
0.25 0.25 -60 to 400/430 7EG-G014-11 Contact Phenomenex or your local
0.32 0.10 -60 to 400/430 7EM-G014-02 Phenomenex distributor for additional
0.32 0.25 -60 to 400/430 7EM-G014-11 GC products and applications.
0.53 0.15 -60 to 400 7EK-G014-05
20-Meter
0.18 0.18 -60 to 400/430 7FD-G014-08 Extend column lifetime.
30-Meter Add a Z-Guard™ to your next
0.25 0.10 -60 to 400/430 7HG-G014-02 Zebron GC order.
0.25 0.25 -60 to 400/430 7HG-G014-11
0.32 0.10 -60 to 400/430 7HM-G014-02
0.32 0.25 -60 to 400/430 7HM-G014-11
0.53 0.15 -60 to 400 7HK-G014-05
Note: If you need a 5 in. cage, contact technical support at www.phenomenex.com/chat or simply
reach out to your Technical consultant. Conditions may apply. Agilent 6850, some SRI and process
GC systems use only 5 in. cages.

Phenomenex 145
 Zebron ™
GC Columns

ZB-5HT Inferno™
Robust Results Up to 430 °C Upgrade to Zebron from any
5 % phenyl / 95 % dimethylpolysiloxane phase:
Agilent® Restek® SGE®
• First non-metal columns stable to 430 °C • DB®-5ht • Rxi®-5HT • HT-5
• Robust column for high temperature bake outs and • VF-5ht • Stx®-5HT
• XTI®-5HT
analysis, such as biodiesel, long-chain hydrocarbons, • Rtx®-5HT
polymers, and high molecular weight compounds
• Provides true boiling point separation for hydrocarbon
distillation methods
• Longer lifetime with rugged high temperature, polyimide
coated, fused silica tubing
• Low activity, provides good peak shape for acidic and basic
samples

Zebron Inferno Columns Win In Paraffin Wax

The Lifetime Test 4th injection
136th injection

How does the lifetime test work?

Only 1.5 % retention difference
All columns were held at 400 °C for 2 hours and then the oven was after
lowered to 120 °C for pentadecane analysis. The VF-5ht column 23 hours in extreme conditions
broke just after 40 hours at 400 °C. The ZB-5HT had the same re-

App ID 16089
tention for pentadecane at 100 hours as the DB-5ht column at 40
hours — over 2X the lifetime!
ZEBRON INFERNO GC COLUMNS | GC COLUMNS

Zebron ZB-5HT Inferno

8 10 12 14 16 18 20 22 min
Agilent DB-5ht
8.5 Agilent VF-5ht
Column: Zebron ZB-5HT Inferno
Dimensions: 15 meter x 0.32 mm x 0.10 μm
Part No.: 7EM-G015-02
8 Injection: On Column @ 43 °C, 0.1 µL
Retention of Pentadecane at 120 °C (min)

Carrier Gas: Helium @ 1.9 mL/min (constant flow)

Oven Program: 40 °C for 2 min to 430 °C @ 20 °C for 10 min
7.5 Detector: FID @ 430 °C
Sample: Paraffin Wax

6.5

6
0 20 40 60 80 100 120
Hours at 400 ˚C

Note that the VF-5ht column died around 40 hours at 400 °C whereas the Zebron ZB-5HT Inferno
column maintained great retention of Pentadecane over 100 hours.

Conditions for all columns:

Dimensions: 30 meter x 0.25 mm x 0.10 μm
Injection: 1.0 μL of test mix AG0-7578
Carrier Gas: Helium @ 1.9 mL/min (constant flow)
Oven Program: 120 °C (Isothermal)
Detector: FID @ 400 °C
Sample: Pentadecane

Comparative separations may not be representative of all applications.

146 Phenomenex
Zebron ™
GC Columns
2007 R&D 100
Award Recipient
ZB-5HT
ZB-5HT Inferno ™
ZB-5HT
Column Profile

Well-Suited for Fuels Analysis

POLARITY MS CERTIFIED

POLARITY MS CERTIFIED

Free Total Glycerin in B100 Biodiesel by GC-FID 8

3 8
USP PHASE TEMP LIMITS ( °C )
6
G27,USP
G36, G41
PHASE -60 toLIMITS
TEMP 400/430*
( °C )
G27, G36, G41 -60 to 400/430*

Improved Glycerol High Sensitivity for Triolein

Resolution Ultra Low Bleed at 380 ˚C

2 4

App ID 16211
7 *0.53
*0.53mm
mmID
ID columns arerated
columns are ratedtoto 400