SAVPUNG AND ANALYSSOF COMMERCIAL FATS AND OLS AOCS% OCS Recommended Pate C147 eves lodine Value of Fats and Oils Cyclohexane Method DEFINITION The dine values «mean of th unssturin of fas and oad is expres in ems ofthe suber affcemigrams of aire sorbed per gram of test ample odin bored. SCOPE Applicable to ll normal ft and oil with iodine vals in rhe rane of 15 1070 tha do not conn ‘nage dbl bends (ce Noes, 1). APPARATUS 1. Gh-oppered iodine Rks—500 ml. 2 Glassoppeed volun laks—100 ml, fr ppg andar slain 3 Volumenicdspenser—25 nL, {ml adjsabily, or Wis sluon. 44. Volume dpenser—25 mL, 1 ml ada, fo 158 prassum odie (0 slion 5, Voluneticspenser—2 mL, 1 nasi fr such solution, 6 Valumec penser 30 rl, {ml jbl, or disiled wae, 7. Repeater pper—wih lig Hack, 20 ml, for ydoherane 1 Angi Balner—aeeure 0.001 g, 9. Magnets 10. Fer paper Whatman #541, orequlent LU. Besken—50 el, 12. Hocaieoren, 13. Tine, REAGENTS 1. Wj solon—accaruey sanded ee Nos 2 and Casio) 2 Pras fd Islan, repre by ng 15g of eagen-gde Klin 100 of ied wae 5, Gpaberane—teage ge ee Now 3nd 4 and Cin. 4 Soule arched frei Ge Nos, 8. Me a pare wi | zofsarch re Nees 6 and srl anu of cold disiledwaee Add, we rng, 20 ml of bailing wae Te frseitviy—Pce5 lof arch olton 10 rf wateranadd 0.05 of fehl prpere0.A MIKI ak tion oe drop a's 50 pe hie olin made by dksing ml commer 5% oi pcre (NOC toon 01000. The drp bic elo rode st be dicharel by 0.05 ml of 1 M our onli 5, Fount dchonate—reagent rade. The pots dichromate Bey god od ded conan weight 2 about UOC belie singe Nts, Caton 6 Hpdcho acid ragent grade, eancensated 5 11.19 (xe Now, Caxton. 7 Sadium those (Ne,8,0, 5,0} M, acum sandal, pepe from rageacgude Na.§,0,5H,0 (ee Now, 7) ‘Sandudinon of edn thinliee—Weigh 0.60.22 of icy ground and deed porasum dichromate into a 500m isk rbd by dierence om a weighing horde isle in 28 ml of wae, ad of concen yd Chloe a, 20 maf poasum add whom, nd tat to mi Allo co tad fot 5 min athena 100 of Akt water. Tt with ia hl slo, shaking coniouly ane he low clr has al appeared ‘Add 1-2 of sec inet an continae the sation, adding the ile sltios sol wat the Bue er ut dzapeas. The sength ofthe sodium dose sluion expres ints of ts lt, Mola of Ns, $,0, solution Paget ot(C4 1b-87 « line Value of Fas and Oils Cyclohexane Method PROCEDURE 1, Male es sample iic isnt ale Lig ie temperate during meng sould no xen the meting point ofthe texsanplely mote than 10, ad fer rough vo pecs of ter paper to emove anys impuries nthe ast ces ‘oftoiure Theron may be perme! in an aioven at 80-851, bu sald be complete within S min #40, The ‘example mu beaboltel dy. oeAI lar mist be able lean and comply dr Afr flan allow he flee es oan achieve a temperate of 8-71 + °C before weighing the ex poton. ‘Once the est potion ha achieved tempentur of 68-71 + MC imeinly weigh he es poton ino 8500 ra. idine th using dhe weighs and eighing acura need in Table 1 Gee Noes 8) ‘Ad 20 eof gelobnane on top of hee orion and wi ro ensue thst the te ponton it comply divaed (xe Nos. 9. 5, Dispense 25 ml. of Wij solution io ask conning the est portion, stopper the Mak and lt ensue an incite mata Immediately tthe tines fo Dor 2.0 depending onthe iin ae of he et poem IV < 150, 1. 1V2 150,20 ree Noe. Store the ak ince dak aa ener of 25 45°C se Nos, 9), Pegure and conduct t leone bank deerminaan wih ech group of west portion simckaneouly and smi ll rapes tothe amore the sis Fom storage and add 20 ml of KI sluin flowed by 100m of dled water ee Notes 10. ‘Tira with 21 MNi5,0, sition, aig it gradu and with constant ad igs chaking ee Noses, 1)-Contnue thetitation und the yl color has amar disappeared, Add 1-2 ml. of sch indieator slain and contin he tation uni he Be lor as jt append CALCULATIONS 1. Thee va agen Whe ‘B= volume of ian, ml. of Hank Ss yolune of any fe poron ‘Mma of N40, cation PRECISION 1. SeeTibe 2 Notes Conon ‘Gohan is amma ana dageous fs sik Is dey vn by inka and shin contac. The TLV in ari 360 pp pslaten csr see urs athe pos can a nga ana icf ad ime Ws soluton witout exten erachloie le comin “Hyhcklare cds omg acd ad wil cae eve bP clothing bol be worn when woking wh his sci Ics toxic by ngesion and iblason andi song iat oe anda, The eof rope open fue hod i ‘eeancnded. When dling thea ary add he acid the wat never he ene Fossum dichromate se by ing nd nhl. Thee len ede in uma he cacogiiy of roma [16 in paul lng cans Ic song ovdlng agen nd dangerous fie ik whes i contac with npc chemical. NUMBERED NOTES 1. Wea the dn vale is determined on materials having cosugated gens, the sls nota mesure of tl stu sin, bu her san enpiial vale indica of the amour of unsuaton prsent, Reproduce sks are obwined hich aod comparison of ol unsaturation. 2. Besse the prepaion ofthe Wisslon i ie consuming and involve the eof bth around roc chem, ‘hs selon may be pursed froma chemi spi. Soltis are arable which eontuin no catbon traci 2nd such solutions should be wed. IW slaions sense wo temperate, mr and light, Soe ins col and dae place and ner low wocome wo 2 emperature bore 25-30°C, 3. rsh qelbesane shuld ke wed. Eric sls maybe obsted ifokd cychhetane ed 4 The AOCS study epoed inf, Ol Chem, Sc. (References I we ccloheane lone. When use jlo sone, she W min acon tine specie inthe omer AOCS yeson ofthe Wis mo sient fo te portions hving sn 13100, epecly sho, aeporedin Rfencs, 1. A sud completed by FOSFA Intemational in 192 (References, 2), homed thr the 80 3861 (138) and ACCS Recommend Practice Cd 1-67 (1993) meth or determining IV da geet“Ran AND AUIS FCOMNETEAT AR NOGA C4 1b-87 + line Value of Fats and Oils Cyclohexane Method ot ge it aes that are sacl difetet, and dha he ws of eles (aloe ia pie of crbonceraclovide ss the salen dos no lfc the observed IV fortron ofwigherid ol having iodine vas eewen 18 and 52 (he ‘ange of edine vals rcouneed ia he sed. Variable esl were bane! in the aplication ofthe method fh ls, however father sis cated out bythe Iteratoral Asociation of Fh Ne Manufactures ndcned that reproducible IV sets cold he bined fhe reaction time ofthe Wij reagent and Fish ol mas etende, 5 19 starch slrion maybe prc fam cher suppie 6 Pato Sarch or laden” is commended, because ths sarc podaces a deep blue cole inthe presence ofthe dor am on “Soluble Sts sn wcomnended because a oasten deep blue oor may ux be devdoped when some ‘oll tach interact withthe Sedona fon. The follwing se stable ches Soable Stach for ladon. Fhe 516-100; Slile Pot Sach, Sigma S 2630; Slule Pour Suh for lodomecr, T-Bar 4006.04, 17. Thesdium thiol lion may he porch fom 4 chemical spplie, Hose ial mest be acarelytandaed ind inthe borate 8, The weight of the st portion mst be suc dha chee wil be an xcs of Wis solution of 50-60% of he amen ade {Ley 100-1508 ofthe amount aboibed 9. the rection noc terminated within 3 min pst the econ ine the est poron mus be dicerded 10, These pon mabe tated within 30 min af reaction ermiatin, afer which the anal sina 1, Mechanical iting is rsommendd far agmon dang heaton of hone REFERENCES 1, AOCS calabortve sly rls sing loberane sone pyar in J Am Oi Chom So 5745 (188) 2. INFORM 3:1246 (1992 Tabled Testsample weights. Terme whe latin vie item ae a ’ ‘ 3 w 10001 3 10576 0s 5 ou 005 0 31730 ‘002 a sues ‘9002 a 35 ‘002 o Sus ‘0co2 i xe ‘avo 0 3073 ‘soon 0 2 ‘oun 10 2266 ont 160 1983 ‘ont 180 76 ‘oma ET 1596 10 ae 4Gd1b-87 + lodine Value of Fats and Oils Cyclohexane Method table2 Theinterabortoy study completed by FOSFA Intemational 1989, i which 3 laboratories patcpated each obtaining fourtest results foreach est portion analyzdby AOCS Recommended Practice Cd 1b-7 (cydeherane) and four tat ‘ests for each test portion analyzed by 150 method 3961 (arbon tetrachlrde, uve the stata rests (evaluated in cordance with 805725-1884sunmarledin the folowing table, Teper Nameaf rine Poa P08 Boa ra Neo ioneas| ¥ a a a avoir ees » ™ 3 3% Ne ef mle » z M4 2 Mean 5209 m7 a9 mis Repeal dion out ons om ‘8 Repeal neice fain) om as 0s am pnd sand deron on oe os a6 Repo eect of vin (8) at on 034 os pea) 0 as 037 an, epi (8) a0 oso Ls a6 Rang ofall sis vrais 07-333 rs8-1855 We 3 22 2) 205 “Teasley 300, RAD plo: PHO, cade pm Keelok age 4