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Order of Draw

The document outlines the basic order of draw followed by most phlebotomy laboratories, listing the different colored tubes used for blood collection and their purposes. It notes that the light blue tube requires a full draw for coagulation assays, while the red tube contains no additives. The document provides instructions for thoroughly mixing the tubes after collection by inverting them a specified number of times, with exceptions for some tubes.

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Glenn Perez
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414 views1 page

Order of Draw

The document outlines the basic order of draw followed by most phlebotomy laboratories, listing the different colored tubes used for blood collection and their purposes. It notes that the light blue tube requires a full draw for coagulation assays, while the red tube contains no additives. The document provides instructions for thoroughly mixing the tubes after collection by inverting them a specified number of times, with exceptions for some tubes.

Uploaded by

Glenn Perez
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

The basic order of draw followed by most phlebotomy laboratories is as follows:

1. Yellow Top (or Yellow-Black). This tube is used for blood cultures.
2. Light Blue. This tube contains sodium citrate, and is used for coagulation assays. A full
draw is required with this tube.
3. Red. This tube does not contain any additives.
4. Gray (with Red) or Gold. This is the serum separator tube (SST) containing a clot
activator and a gel separator.
5. Dark Green. This tube contains sodium heparin anticoagulant.
6. Light Green. This is the plasma separating tube (PST) containing lithium heparin
anticoagulant and a gel separator.
7. Lavender. This tube contains EDTA (Ethylenediaminetetraacetic acid).
8. Pale Yellow. This tube contains acid citrate dextrose.
9. Light Gray. This tube contains oxalate or fluoride.

Each tube should be thoroughly mixed by inverting the tube 8-10 times. Exceptions to this rule
are the Light Blue tube, which is to be mixed by inverting 3-4 times, and the Red tubes (non-
additive and SST), which are to be mixed by inverting 5 times.

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